U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal Biologia plantarum Remove constraint Journal: Biologia plantarum Publication year rev 7990-2010 Remove constraint Publication year rev: 7990-2010
Start Over

Search

141  Search Results

« Previous | 1 - 100 of 141 |
Number of results to display per page

Search Results

1. 2D random walk representation of Begonia × tuberhybrida multiallelic loci used for germplasm identification

Author:
Wiesner, I.; Wiesnerová, D.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 353-356
ISSN:
0006-3134
Subject:
Begonia; DNA; alleles; genetic markers; genetic variation; germplasm; loci; nucleotide sequences; principal component analysis; sequence analysis
Abstract:
... In this study, we wanted to inspect whether the evolutionary driven differences in primary sequences could correlate, and thus predict the genetic diversity of related marker loci, which is an important criterion to assess the quality of any DNA marker. We adopted new approach of quantitative symbolic DNA sequence analysis called DNA random walk representation to study multiallelic marker loci fro ...
DOI:
10.1007/s10535-010-0062-7

2. Acetylsalicylic acid ameliorates negative effects of NaCl or osmotic stress in Solanum stoloniferum in vitro

Author:
Daneshmand, F.; Arvin, M. J.; Kalantari, K. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 781-784
ISSN:
0006-3134
Subject:
electrolytes; ascorbate peroxidase; lipid peroxidation; drought; polyethylene glycol; potatoes; aspirin; hydrogen peroxide; explants; sodium; peroxidase; osmotic stress; linoleate 13S-lipoxygenase; superoxide dismutase; physiological response; potassium; sodium chloride; adverse effects; glutathione-disulfide reductase; catalase; salinity; Solanum stoloniferum
Abstract:
... The role of acetylsalicylic acid (0, 1 and 10 μM) pre-treatment in amelioration of salt and osmotic stress in a wild species of potato (Solanum stoloniferum) was investigated. We compared the effects of iso-osmotic concentrations of polyethylene glycol 6000 (15 %) and NaCl (80 mM) on the physiological responses of this species in explants grown in the liquid Murashige and Skoog medium. Both salt a ...
DOI:
10.1007/s10535-010-0142-8

3. Activities of antioxidant enzymes during strawberry fruit development and ripening

Author:
López, A. P.; Gochicoa, M. T. N.; Franco, A. R.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 349-352
ISSN:
0006-3134
Subject:
catalase; enzyme activity; fruiting; fruits; peroxidase; ripening; strawberries; superoxide dismutase
Abstract:
... The analyses of some antioxidant enzyme activities were carried out in the course of strawberry fruits development and ripening. The catalase activity was maximum in small-sized green fruits, it decreased in middle-sized green fruits and increased again during the ripening stages. The highest superoxide dismutase and peroxidase activities were observed in white fruits. ...
DOI:
10.1007/s10535-010-0061-8

4. Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus

Author:
Vatankhah, E.; Niknam, V.; Ebrahimzadeh, H.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 509-514
ISSN:
0006-3134
Subject:
Crocus sativus; callus; catalase; catechol oxidase; enzyme activity; kinetin; naphthaleneacetic acid; organogenesis; peroxidase; protein content; roots; shoots; sprouting; superoxide dismutase; thidiazuron
Abstract:
... The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm⁻³ 1-naphthaleneacetic acid (NAA) + 1.0 mg dm⁻³ thidiazuron and 1.0 mg dm⁻³ NAA + 2.0 mg dm⁻³ kinetin. For sprouting, regenerated shoot were subcultured on Mu ...
DOI:
10.1007/s10535-010-0089-9

5. Allelochemical effects on net nitrate uptake and plasma membrane H⁺-ATPase activity in maize seedlings

Author:
Abenavoli, M. R.; Lupini, A.; Oliva, S.; Sorgonà, A.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 149-153
ISSN:
0006-3134
Subject:
H-transporting ATP synthase; caffeic acid; corn; hydroponics; p-coumaric acid; plasma membrane; potassium nitrate; seedlings; umbelliferones
Abstract:
... Seven-day-old maize seedlings grown in a nitrogen-free hydroponic culture were exposed for 48 h to 0, 100 and 300 μM trans-cinnamic, p-coumaric, ferulic, caffeic acids, umbelliferone and 200 μM KNO₃. Net nitrate uptake was affected by trans-cinnamic, ferulic and p-coumaric acids in a concentration-dependent manner, and trans-cinnamic acid appeared to be the strongest inhibitor. Conversely, at low ...
DOI:
10.1007/s10535-010-0024-0

6. Analysis of Lupinus albus heat-shock granule proteins in response to high temperature stress

Author:
Cherian, S.; Ferreira, R. B.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 587-591
ISSN:
0006-3134
Subject:
Lupinus albus; gene expression regulation; granules; heat; heat shock proteins; heat shock response; heat stress; molecular weight; two-dimensional gel electrophoresis
Abstract:
... An important aspect of heat-shock response of lupin (Lupinus albus cv. Rio Maior) is the formation of cytoplasmic granular aggregates, called heat-shock granules (HSGs). In this study, two-dimensional electrophoresis (2-DE) was used to detect the component proteins of HSG complexes formed in vivo. Evaluation of 2-DE revealed differential expression of several proteins under heat shock conditions w ...
DOI:
10.1007/s10535-010-0106-z

7. Analysis of the role of mitochondrial and endoplasmic reticulum localized small heat shock proteins in tomato

Author:
Nautiyal, P. C.; Shono, M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 715-719
ISSN:
0006-3134
Subject:
Solanum lycopersicum var. lycopersicum; biomass; cell membranes; endoplasmic reticulum; genetically modified organisms; heat; heat shock proteins; heat stress; mitochondria; photosynthesis; protein synthesis; temperature; tomatoes
Abstract:
... This communication examines the role of small heat shock proteins (sHsps) targeted to mitochondria (Mt) and endoplasmic reticulum (ER) in tomato plants (Lycopersicon esculentum Mill.) under heat stress. Genetic response of transgenic and wild type plants varied under optimum, moderately elevated and elevated temperature. In optimum temperature higher biomass was recorded in wild type than the tran ...
DOI:
10.1007/s10535-010-0127-7

8. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources

Author:
Vuletić, M.; Hadži-Tašković Šukalović, V.; Marković, K.; Dragišić Maksimović, J.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 530-534
ISSN:
0006-3134
Subject:
Zea mays; ascorbate oxidase; ascorbate peroxidase; corn; enzyme activity; genotype; isoelectric focusing; nitrates; nitrogen; osmotic stress; peroxidase; phenolic compounds; polyethylene glycol; proline; roots; superoxide dismutase
Abstract:
... The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO₃ ⁻) or both nitrogen forms, nitrate and ammonium (NH₄ ⁺/NO₃ ⁻). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline ...
DOI:
10.1007/s10535-010-0093-0

9. Assessment of genetic diversity and relationships among Coix lacryma-jobi accessions using microsatellite markers

Author:
Ma, K. -H.; Kim, K. -H.; Dixit, A.; Chung, I. -M.; Gwag, J. -G.; Kim, T. -S.; Park, Y. -J.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 272-278
ISSN:
0006-3134
Subject:
Coix lacryma-jobi; algorithms; alleles; genetic background; genetic markers; genetic relationships; genetic variation; heterozygosity; loci; China; Korean Peninsula
Abstract:
... The present study describes the assessment of genetic diversity and relationships among 79 Job's tears (Coix lacrymajobi L.) accessions collected from China and Korea using 17 microsatellite markers. A total of 57 alleles were detected with an average of 3.4 alleles per locus. A high frequency of rare alleles (36.3 %) was observed within the collection. Values for observed (HO), expected heterozyg ...
DOI:
10.1007/s10535-010-0047-6

10. Carbon nutrition of mature green orchid Serapias strictiflora and its mycorrhizal fungus Epulorhiza sp

Author:
Látalová, K.; Baláž, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 97-104
ISSN:
0006-3134
Subject:
Orchidaceae; autotrophs; carbon; corn; mycelium; mycorrhizal fungi; nutrition; organic compounds; roots; saprotrophs; tubers
Abstract:
... We studied the nutritional modes of the orchid Serapias strictiflora and its mycorrhizal fungus Epulorhiza sp. using the differences in carbon isotopic composition (δ¹³C) of C₃ orchid and C₄ maize tissues. We found that if cultivated in substrate lacking any organic compounds, the mycorrhizal extraradical mycelia (δ¹³C = −26.3 ± 0.2 ‰) developed well, despite being fully dependent on nutrition fro ...
DOI:
10.1007/s10535-010-0014-2

11. Changes in AFLP and SSR DNA polymorphisms induced by short-term space flight of rice seeds

Author:
Lu, J. Y.; Zhang, W. L.; Xue, H.; Pan, Y.; Zhang, C. H.; He, X. H.; Liu, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 112-116
ISSN:
0006-3134
Subject:
DNA; DNA primers; Oryza sativa; amplified fragment length polymorphism; flight; genetic polymorphism; leaves; microsatellite repeats; rice; seeds; space flight
Abstract:
... Differences of both amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) polymorphisms were compared between the 60-d-old rice (Oryza sativa L. cv. DH7) and F3 rice plants (SP3) derived from seed, which endured a 7-d-space flight in March 2002. Total leaf AFLP DNA bands amplified from 22 primer pairs were 537 in DH7, whereas 562 in SP3. From the total 267 SSR DNA bands ge ...
DOI:
10.1007/s10535-010-0016-0

12. Characteristics of chlorophyll fluorescence and antioxidative system in super-hybrid rice and its parental cultivars under chilling stress

Author:
Zhang, Y. H.; Chen, L. J.; He, J. L.; Qian, L. S.; Wu, L. Q.; Wang, R. F.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 164-168
ISSN:
0006-3134
Subject:
Oryza sativa; ascorbate peroxidase; chlorophyll; cold tolerance; cooling; cultivars; fluorescence; genotype; glutathione-disulfide reductase; photosystem II; rice; superoxide dismutase
Abstract:
... Characteristics of chlorophyll fluorescence and antioxidative system were investigated in rice (Oryza sativa L.) super-hybrid Liangyoupeijiu (LYPJ), maternal cultivar Peiai64s, and paternal cultivar indica rice 9311 under chilling stress. During 6-d chilling treatment, chlorophyll content of all three genotypes was gradually declined. However, the decrease in photosystem 2 (PS 2) maximum photochem ...
DOI:
10.1007/s10535-010-0027-x

13. Characterization of a xylanase inhibitor TAXI-I from wheat

Author:
Weng, X. -Y.; Huang, Y. -Y.; Gao, H.; Sun, J. -Y.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 154-158
ISSN:
0006-3134
Subject:
Aspergillus niger; Bacillus subtilis; Escherichia coli; Thermobifida fusca; Triticum aestivum; bacteria; fungi; gene expression; genes; gibberellic acid; glycosides; hybrids; methyl jasmonate; parents; wheat; xylanases
Abstract:
... Xylanase inhibitor TAXI-I gene was cloned from wheat (Triticum aestivum L.) and then TAXI-I encoding sequence was expressed in Escherichia coli. The recombinant TAXI-I protein inhibited glycoside hydrolase (GH) family 11 xylanases in Aspergillus niger (Anx; a fungal xylanase), and Thermomonospora fusca (Tfx; a bacterial xylanase), and also inhibited hybrid xylanases Atx (a hybrid xylanase whose pa ...
DOI:
10.1007/s10535-010-0025-z

14. Characterization of the antioxidant system during the vegetative development of pea plants

Author:
Díaz-Vivancos, P.; Barba-Espín, G.; Clemente-Moreno, M. J.; Hernández, J. A.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 76-82
ISSN:
0006-3134
Subject:
antioxidants; ascorbate oxidase; ascorbate peroxidase; catalase; enzyme activity; glutathione; glutathione dehydrogenase (ascorbate); monodehydroascorbate reductase (NADH); peas; peroxidase; roots; seedlings; shoots; superoxide dismutase; tissues
Abstract:
... The antioxidative system was studied during the development of pea plants. The reduced glutathione (GSH) content was higher in shoots than in roots, but a greater redox state of glutathione existed in roots compared with shoots, at least after 7 d of growth. The 3-d-old seedlings showed the highest content of oxidised ascorbate (DHA), which correlated with the ascorbate oxidase (AAO) activity. Als ...
DOI:
10.1007/s10535-010-0011-5

15. Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances

Author:
Osório, M. L.; Osório, J.; Romano, A.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 415-422
ISSN:
0006-3134
Subject:
Rhododendron ponticum; acclimation; chlorophyll; electron transfer; energy; fluorescence; light intensity; micropropagation; photoinhibition
Abstract:
... The aim of this study was to investigate acclimation of micropropagated plants of Rhododendron ponticum subsp. baeticum to different irradiances and recovery after exposure to high irradiance. Plants grown under high (HL) or intermediate (IL) irradiances displayed higher values of maximum electron transport rate (ETRmax) and light saturation coefficient (Ek) than plants grown under low irradiance ...
DOI:
10.1007/s10535-010-0076-1

16. Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis

Author:
Lu, X.; Zhou, W.; Gao, F.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 798-800
ISSN:
0006-3134
Subject:
Citrus sinensis; anthocyanins; biosynthesis; chromosomes; fluorescence in situ hybridization; genes; karyotyping; metaphase; oranges; ribosomal DNA
Abstract:
... Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S r ...
DOI:
10.1007/s10535-010-0146-4

17. Cold tolerance, water relations and accumulation of osmolytes in Bituminaria bituminosa

Author:
Walker, D. J.; Romero, P.; Correal, E.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 293-298
ISSN:
0006-3134
Subject:
Bituminaria bituminosa; cold; cold tolerance; electrolytes; freezing; leaves; osmotic pressure; osmotolerance; proline; shrubs; spring; sugars; temperature; winter
Abstract:
... We determined the cold (freezing) tolerance of five Spanish populations of the perennial shrub Bituminaria bituminosa (L.) C.H. Stirton (Fabaceae), as the temperature at which 50 % of leaf electrolytes are released (LT₅₀) using leaves of field-grown plants, obtained in two winters and one spring. The freezing tolerance was greater in winter and reflected the minimum temperatures at the original si ...
DOI:
10.1007/s10535-010-0051-x

18. Comparison of three genetic similarity coefficients based on dominant markers from predominantly self-pollinating species

Author:
Beharav, A.; Maras, M.; Kitner, M.; Šuštar-Vozlič, J.; Sun, G. L.; Doležalová, I.; Lebeda, A.; Meglič, V.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 54-60
ISSN:
0006-3134
Subject:
DNA; Lactuca saligna; Phaseolus vulgaris; Triticum turgidum subsp. dicoccoides; amplified fragment length polymorphism; beans; genetic variation; lettuce; population structure; random amplified polymorphic DNA technique; self-pollination
Abstract:
... Three genetic similarity coefficients were estimated and compared for their usefulness: simple matching (S SM), Jaccard's (S J) and Dice's (S D), all based on dominant markers data from individuals representing predominantly self-pollinating species. AFLP markers were used to analyze 139 Phaseolus vulgaris L. (common bean) and 67 Lactuca saligna L. (least lettuce) accessions, and RAPD markers were ...
DOI:
10.1007/s10535-010-0008-0

19. Concurrent occurrence of α-amylase inhibitor and stimulator in red kidney bean seed: physiological implications

Author:
Alizadeh, H.; Leung, D. W. M.; Cole, A. L. J.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 195-197
ISSN:
0006-3134
Subject:
phytohemagglutinin; kidney beans; cotyledons; Phaseolus vulgaris; alpha-amylase
Abstract:
... It is hypothesized that since protein α-amylase inhibitor (α-AI) and stimulator might be present together in red kidney bean (Phaseolus vulgaris L.) seeds, their in vitro interactions might influence their detection and quantification. Assay of α-AI using extracts from the embryonic axes revealed an unexpected finding in that the extracts stimulated rather than inhibited α-amylase activity. The co ...
DOI:
10.1007/s10535-010-0034-y

20. Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization

Author:
Mirzaghaderi, G.; Karimzadeh, G.; Hassani, H. S.; Jalali-Javaran, M.; Baghizadeh, A.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 252-258
ISSN:
0006-3134
Subject:
chromosome banding; chromosome number; chromosomes; crossing; cultivars; cytogenetic analysis; genotype; hybrids; in situ hybridization; meiosis; salt tolerance; seeds; wheat
Abstract:
... The new salt tolerant cereal, Tritipyrum (2n=6x=42, AABBEbEb) offers potential to introduce desirable characters for wheat improvements. This study was aimed to generate a segregating population from Iranian local wheat cultivars (2n=6x=42, AABBDD) and Tritipyrum crosses, study of the meiotic behaviour in F₂ hybrids and identification of Eb chromosomes in F₃ individuals. Results showed meiotic abn ...
DOI:
10.1007/s10535-010-0044-9

21. Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber

Author:
Diao, W. -P.; Bao, S. -Y.; Jiang, B.; Cui, L.; Qian, C. -T.; Chen, J. -F.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 373-376
ISSN:
0006-3134
Subject:
Cucumis sativus; anaphase; chromosome segregation; chromosomes; cucumbers; cytokinesis; gametophytes; germination; meiosis; metaphase; pollen; telophase
Abstract:
... With improved staining and chromosome preparation techniques, meiosis of pollen mother cells (PMCs) and male gametophyte development in autotetraploid cucumber (Cucumis sativus L.) was studied to understand the correlation between chromosomes behaviour and fertility. Various chromosome configurations, e.g. multivalent, quadrivalents, trivalents, bivalents and univalents were observed in most PMCs ...
DOI:
10.1007/s10535-010-0067-2

22. Development of AFLP and STS markers linked to a waterlogging tolerance in Korean soybean landraces

Author:
Kang, S. -Y.; Lee, K. J.; Lee, G. -J.; Kim, J. -B.; Chung, S. -J.; Song, J. Y.; Lee, B. -M.; Kim, D. S.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 61-68
ISSN:
0006-3134
Subject:
DNA primers; Glycine max; amplified fragment length polymorphism; chlorophyll; cluster analysis; flooded conditions; gamma radiation; genotype; glucose; landraces; malondialdehyde; marker-assisted selection; mutants; mutation; soybeans
Abstract:
... Among the 400 soybean (Glycine max) landraces, we selected 3 tolerant (KAS150-9, KAS160-15, and KAS170-9) and 3 susceptible lines (KAS160-14, KAS160-20, and KAS201-6-1) by the survival percentage and injury scores. Susceptible lines showed decrease in chlorophyll content and increase in glucose and malondialdehyde (MDA) contents under waterlogging stress, while tolerant lines did not change signif ...
DOI:
10.1007/s10535-010-0009-z

23. Direct plant regeneration from encapsulated nodal segments of Vitex negundo

Author:
Ahmad, N.; Anis, M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 748-752
ISSN:
0006-3134
Subject:
Vitex negundo; acetic acid; artificial seeds; calcium chloride; concentration of production; culture media; encapsulation; germination; kinetin; naphthaleneacetic acid; plantlets; sodium alginate
Abstract:
... An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na₂-alginate with 100 mM CaCl₂ has been found to be optimum concentration for the production of uniform synthetic ...
DOI:
10.1007/s10535-010-0134-8

24. Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

Author:
Khurana-Kaul, V.; Kachhwaha, S.; Kothari, S. L.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 369-372
ISSN:
0006-3134
Subject:
Jatropha curcas; benzyladenine; buds; callus; callus formation; copper sulfate; explants; gibberellic acid; indole butyric acid; leaves; plantlets; shoots; thidiazuron
Abstract:
... Leaf explants of Jatropha curcas cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ; 0.90 μM) in combination with indole-3-butyric acid (IBA; 0.98μM) produced adventitious shoot buds directly on the surface of the explants without formation of intervening callus while shoot bud formation was accompanied with callus formation on medium supplemented with 6-benzylaminopu ...
DOI:
10.1007/s10535-010-0066-3

25. Direct somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum

Author:
Ma, G. H.; He, C. X.; Ren, H.; Zhang, Q. M.; Li, S. J.; Zhang, X. H.; Eric, B.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 361-365
ISSN:
0006-3134
Subject:
acclimation; activated carbon; adventitious roots; adventitious shoots; benzyladenine; cytokinins; endangered species; explants; indole butyric acid; kinetin; leaves; organogenesis; pH; plantlets; rooting; somatic embryogenesis; somatic embryos; thidiazuron
Abstract:
... An efficient propagation system via somatic embryogenesis and shoot organogenesis and plant regeneration system for endangered species Primulina tabacum Hance was established. Thidiazuron (TDZ) was the key plant growth regulator for inducing somatic embryogenesis and kinetin (KIN) and 6-benzylaminopurine (BAP) were the key cytokinins for inducing shoot organogenesis from leaf explants. TDZ combine ...
DOI:
10.1007/s10535-010-0064-5

26. An EST-SSR marker linked with yellow rust resistance in wheat

Author:
Ercan, S.; Ertugrul, F.; Aydin, Y.; Akfirat, F. S.; Hasancebi, S.; Akan, K.; Mert, Z.; Bolat, N.; Yorgancilar, O.; Altinkut-Uncuoglu, A.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 691-696
ISSN:
0006-3134
Subject:
DNA; Triticum aestivum; expressed sequence tags; genetic markers; genotype; hybrids; microsatellite repeats; seedlings; stripe rust; wheat
Abstract:
... Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F₂ seedlings were constructed. 78 EST-SSR prime ...
DOI:
10.1007/s10535-010-0122-z

27. Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants

Author:
Mýtinová, Z.; Motyka, V.; Haisel, D.; Gaudinová, A.; Lubovská, Z.; Wilhelmová, N.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 461-470
ISSN:
0006-3134
Subject:
Arabidopsis thaliana; Nicotiana tabacum; abscisic acid; antioxidants; ascorbate peroxidase; catalase; cytokinins; enzyme activity; genes; glutathione-disulfide reductase; growth retardation; indole acetic acid; leaves; phenotype; promoter regions; roots; salinity; superoxide dismutase; tobacco; transgenic plants; water stress; zinc
Abstract:
... The responses of antioxidant enzymes (AOE) ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in soluble protein extracts from leaves and roots of tobacco (Nicotiana tabacum L. cv. Samsun NN) plants to the drought stress, salinity and enhanced zinc concentration were investigated. The studied tobacco included wild-type (WT) and transgenic plants ...
DOI:
10.1007/s10535-010-0082-3

28. Effect of photoperiod during growth of Chenopodium rubrum mother plants on properties of offspring

Author:
Mitrović, A.; Bogdanović, J.; Giba, Z.; Ćulafić, L.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 735-739
ISSN:
0006-3134
Subject:
Chenopodium rubrum; flowering; in vitro culture; mothers; photoperiod; progeny; seed germination; seed maturation
Abstract:
... Using in vitro culture, we determined the effect of photoperiod during growth of Chenopodium rubrum mother plants on vegetative and reproductive development of offspring. Photoperiod during flowering induction of mother plants (the first 6 d after the germination) has the key influence on seed germination and offspring growth, while offspring flowering and seed maturation is determined by photoper ...
DOI:
10.1007/s10535-010-0131-y

29. Effect of salicylic acid pretreatment on cadmium toxicity in wheat

Author:
Moussa, H. R.; El-Gamal, S. M.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 315-320
ISSN:
0006-3134
Subject:
Triticum aestivum; abscisic acid; biomass; cadmium; chlorophyll; chloroplasts; hydrogen peroxide; malondialdehyde; phosphoenolpyruvate carboxylase; proline; root growth; roots; salicylic acid; seeds; toxicity; ultrastructure; water content; wheat
Abstract:
... Cadmium (100, 400 and 1000 μM CdCl₂) treatments resulted in the inhibition of root dry biomass, root elongation and increased Cd accumulation in wheat (Triticum aestivum L.) roots. Further, these treatments decreased relative water content, chlorophyll content, ¹⁴CO₂-fixation, activities of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase and abscisic acid content while in ...
DOI:
10.1007/s10535-010-0054-7

30. Effect of zinc and cadmium on physiological and production characteristics in Matricaria recutita

Author:
Kummerová, M.; Zezulka, Š.; Kráľová, K.; Masarovičová, E.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 308-314
ISSN:
0006-3134
Subject:
Matricaria chamomilla; cadmium; carotenoids; chamomile; chlorophyll; fluorescence; hydroponics; pigments; respiratory rate; roots; shoots; zinc
Abstract:
... Effects of zinc (12-180 μM) alone and in mixtures with 12 μM Cd on metal accumulation, dry masses of roots and shoots, root respiration rate, variable to maximum fluorescence ratio (FV/FM), and content of photosynthetic pigments were studied in hydroponically cultivated chamomile (Matricaria recutita) plants. The content of Zn in roots and shoots increased with the increasing external Zn concentra ...
DOI:
10.1007/s10535-010-0053-8

31. Effects of exogenous spermine on sweet sorghum during germination under salinity

Author:
Chai, Y. Y.; Jiang, C. D.; Shi, L.; Shi, T. S.; Gu, W. B.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 145-148
ISSN:
0006-3134
Subject:
Sorghum bicolor; catalase; germination; glutathione-disulfide reductase; growth retardation; malondialdehyde; peroxidase; proline; protein content; roots; salinity; seedlings; shoots; sodium chloride; spermine; sweet sorghum
Abstract:
... Seedlings of Sorghum bicolor (L.) Moench were subjected to 180 mM NaCl with or without 0.25 mM spermine (SPM) for 7 d. NaCl treatment resulted in the inhibition of growth and increased the content of free proline, soluble protein and malondialdehyde (MDA). Additionally, it also enhanced the activity of catalase (CAT), peroxidase (POX) in both shoots and roots, while decreased that of glutathione r ...
DOI:
10.1007/s10535-010-0023-1

32. Efficient in vitro plant regeneration from shoot apices and gene transfer by particle bombardment in Jatropha curcas

Author:
Purkayastha, J.; Sugla, T.; Paul, A.; Solleti, S. K.; Mazumdar, P.; Basu, A.; Mohommad, A.; Ahmed, Z.; Sahoo, L.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 13-20
ISSN:
0006-3134
Subject:
Cauliflower mosaic virus; Jatropha curcas; Southern blotting; beta-glucuronidase; culture media; explants; gene transfer; genetic transformation; gibberellic acid; gold; kanamycin; osmotic treatment; particle size; plant establishment; plantlets; plasmids; polymerase chain reaction; shoots; transgenes; transgenic plants
Abstract:
... An efficient and reproducible in vitro plant regeneration system from shoot apices was developed in Jatropha curcas. Benzylaminopurine (BAP; 2.5 μM) was most effective in inducing an average of 6.2 shoots per shoot apex. Incorporation of gibberellic acid (GA3; 0.5 μM) to basal medium was found essential for elongation of shoots. The BAP-habituated mother explants continuously produced shoots durin ...
DOI:
10.1007/s10535-010-0003-5

33. Efficient regeneration of Eucalyptus urophylla from seedling-derived hypocotyls

Author:
Huang, Z. -C.; Zeng, F. -H.; Lu, X. -Y.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 131-134
ISSN:
0006-3134
Subject:
Eucalyptus urophylla; benzyladenine; buds; gibberellic acid; greenhouses; hypocotyls; indole acetic acid; indole butyric acid; naphthaleneacetic acid; plantlets; rooting; seedlings; shoots; urea
Abstract:
... Seedling hypocotyls were used as explants to establish a regeneration protocol for Eucalyptus urophylla and N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl] urea (PBU), one kind of di-substituted urea, was found useful growth regulator. The hypocotyls incubated on a modified Murashige and Skoog medium (SPCa), supplemented with 6.6 μM PBU and 0.57 μM indole-3-acetic acid (IAA) dedifferentiated and form ca ...
DOI:
10.1007/s10535-010-0020-4

34. Endogenous brassinosteroids in wheat treated with 24-epibrassinolide

Author:
Janeczko, A.; Swaczynová, J.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 477-482
ISSN:
0006-3134
Subject:
brassinolide; high performance liquid chromatography; leaves; mass spectrometry; plant age; seed treatment; seedlings; seeds; soaking; spraying; wheat
Abstract:
... The aim of the study was to examine the effect of exogenous 24-epibrassinolide on its uptake and content of endogenous brassinosteroids in wheat seedlings. 24-Epibrassinolide was applied at two concentrations (0.1 and 2.0 μM) and in three different methods: by soaking seeds, by drenching and by spraying plants. Brassinosteroids were determined by high-performance liquid chromatography combined wit ...
DOI:
10.1007/s10535-010-0084-1

35. Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor

Author:
Aragón, C.; Carvalho, L.; González, J.; Escalona, M.; Amâncio, S.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 237-244
ISSN:
0006-3134
Subject:
Musa; acclimation; ascorbate peroxidase; bioreactors; catalase; genes; glutathione dehydrogenase (ascorbate); glutathione transferase; glutathione-disulfide reductase; leaves; micropropagation; monodehydroascorbate reductase (NADH); nitroblue tetrazolium; plantlets; polymerization; superoxide dismutase; transgenic plants
Abstract:
... Plantain (Musa ABB CEMSA 3/4) plantlets were micropropagated in temporary immersion bioreactors (TIB) or in gelled medium (GM). After ex vitro transfer ROS accumulation was determined by infiltrating leaves with nitroblue tetrazolium (NBT) and 3,3′-diaminobenzidine (DAB). Stomatal cells were more stained with NBT and DAB in GM plants than in TIB plants, but the difference disappeared at the end of ...
DOI:
10.1007/s10535-010-0042-y

36. Exogenously-supplied trehalose protects thylakoid membranes of winter wheat from heat-induced damage

Author:
Luo, Y.; Li, F.; Wang, G. P.; Yang, X. H.; Wang, W.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 495-501
ISSN:
0006-3134
Subject:
winter wheat; proteins; electrolytes; malondialdehyde; thylakoids; hydrogen peroxide; seedlings; linoleate 13S-lipoxygenase; superoxide anion; heat stress; trehalose; photosynthesis
Abstract:
... The effects of trehalose pretreatment on thylakoid membranes of winter wheat were investigated under heat stress. Under normal growth conditions, the winter wheat synthesized 502 μg g⁻¹(f.m.) trehalose, which increased to 1250 μg g⁻¹(f.m.) under heat stress and to 1658 μg g⁻¹(f.m.) in trehalose-pretreated seedlings. Under heat stress, proteins in the thylakoid membranes and the photosynthetic capa ...
DOI:
10.1007/s10535-010-0087-y

37. Expressions and dimerization affinities of three highly identical APETALA3 genes in Brassica napus

Author:
Zhou, L.; Zhou, Y. -T.; Wang, M. -L.; Wang, H. -Y.; Zhao, Y.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 33-40
ISSN:
0006-3134
Subject:
Brassica napus; amino acid sequences; clones; complementary DNA; corolla; dimerization; gene expression; genes; open reading frames; proteins; reverse transcriptase polymerase chain reaction; sequence analysis; stamens; transcription (genetics); two hybrid system techniques
Abstract:
... Three highly identical cDNA clones of APETALA3 (AP3) gene, BnAP3-2, BnAP3-3 and BnAP3-4 were isolated from Brassica napus L. by RT-PCR. The sequence analysis showed that all the three AP3 cDNAs contained a complete open reading frame. Their nucleotide sequences had 91-97 % similarity and their predicted amino acid sequences shared 93-98 % identity. Real-time quantitative RT-PCR result showed that ...
DOI:
10.1007/s10535-010-0005-3

38. Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Author:
Kumar, A.; Aggarwal, D.; Gupta, P.; Reddy, M. S.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 601-606
ISSN:
0006-3134
Subject:
Chlorophytum borivilianum; DNA; agar; benzyladenine; culture media; cytokinins; gelation; gelling agents; heat; humidity; indole butyric acid; kinetin; light intensity; microsatellite repeats; random amplified polymorphic DNA technique; rooting; shoots; soil; sucrose; temperature; vermicomposts
Abstract:
... The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116-290 mM in the basal medium (BM) promoted shoot multipl ...
DOI:
10.1007/s10535-010-0109-9

39. Fluoride affects distribution of absorbed excitation energy more in favour of photosystem 1

Author:
Singh-Rawal, P.; Jajoo, A.; Bharti, S.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 556-560
ISSN:
0006-3134
Subject:
absorption; ambient temperature; electron transfer; electron transport chain; energy; fluorescence; photosystem I; spinach; thylakoids
Abstract:
... The effects of fluoride on the photosynthetic electron transport chain have been studied in spinach thylakoid membranes. Inhibition in photosystem (PS) 2 electron transport rates and a subsequent increase in PS 1 electron transport rate indicated a possibility of state transitions being a mechanism of fluoride action. This hypothesis was further confirmed by the increase in fluorescence emission F ...
DOI:
10.1007/s10535-010-0099-7

40. Functional characterization of the apple MdSAMDC2 gene by ectopic promoter analysis and over-expression in tobacco

Author:
Zhao, L. -L.; Song, L. -Q.; You, C. -X.; Moriguchi, T.; Hao, Y. -J.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 631-638
ISSN:
0006-3134
Subject:
adenosylmethionine decarboxylase; apples; catalase; cold stress; drought; gene expression regulation; gene overexpression; lipid peroxidation; malondialdehyde; polyamines; polyethylene glycol; promoter regions; reporter genes; sodium chloride; superoxide dismutase; temperature; tobacco; transcription (genetics); transgenic plants
Abstract:
... The expression of MdSAMDC2 gene, which encodes S-adenosylmethionine decarboxylase (SAMDC) in apple, was up-regulated by low temperature, salt and drought stresses. To identify its in vivo biological functions in the responses to stresses, the promoter region of MdSAMDC2 was isolated and characterized by analyzing the cis-acting regulatory elements and GUS reporter gene by an Agrobacterium-mediated ...
DOI:
10.1007/s10535-010-0113-0

41. Genetic diversity in Passiflora species determined by morphological and molecular characteristics

Author:
Viana, A. J. C.; Souza, M. M.; Araújo, I. S.; Corrêa, R. X.; Ahnert, D.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 535-538
ISSN:
0006-3134
Subject:
Passiflora; cluster analysis; flowers; fruits; genetic distance; genetic variation; intraspecific variation; leaf area; random amplified polymorphic DNA technique; seeds
Abstract:
... Morphological and molecular characteristics were studied in six wild species of Passiflora. There were statistically significant differences among these six species for all characteristics studied. Intra-specific variability was observed for number of flowers, number of fruits, number of seeds, fruit length, fruit width and leaf area. Cluster analysis using morphological data showed three groups: ...
DOI:
10.1007/s10535-010-0094-z

42. Genetic diversity of elite sweet sorghum genotypes assessed by SSR markers

Author:
Pei, Z.; Gao, J.; Chen, Q.; Wei, J.; Li, Z.; Luo, F.; Shi, L.; Ding, B.; Sun, S.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 653-658
ISSN:
0006-3134
Subject:
Sorghum bicolor subsp. bicolor; alleles; chromosomes; cluster analysis; genetic variation; genotype; microsatellite repeats; sweet sorghum
Abstract:
... To determine genetic diversity among 47 elite sweet sorghum (Sorghum bicolor ssp. bicolor L.) genotypes, 46 simple sequence repeat (SSR) markers evenly distributed on all 10 chromosomes were selected. All SSR markers used were polymorphic among the genotypes studied. A total of 228 alleles were identified with an average of 4.96 alleles per marker. Furthermore, the genotypes studied showed medium ...
DOI:
10.1007/s10535-010-0116-x

43. Genetic relatedness in different Medicago sativa lines based on polymorphism of mitochondrial DNA specific sequences

Author:
Skuza, L.; Dyba, S. M.; Rogalska, S. M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 169-172
ISSN:
0006-3134
Subject:
Medicago sativa; alfalfa; genetic relationships; inbred lines; landraces; mitochondrial DNA; nucleotide sequences; seed set; soybeans; synthetic populations
Abstract:
... Two synthetic populations (Syn-5 and Syn-7), four inbred lines and four landraces of alfalfa (Medicago sativa L.) were studied for genetic relatedness. These forms were characterized by different amount of seeds set and green mass yield. Two primer pairs of specific nucleotide sequences of mitochondrial DNA isolated from soybean were used. The mtDNA revealed the existence of significant polymorphi ...
DOI:
10.1007/s10535-010-0028-9

44. Gibberellin retards chlorophyll degradation during senescence of Paris polyphylla

Author:
Li, J. R.; Yu, K.; Wei, J. R.; Ma, Q.; Wang, B. Q.; Yu, D.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 395-399
ISSN:
0006-3134
Subject:
proteins; perennials; gibberellic acid; linoleate 13S-lipoxygenase; chlorophyll; peroxidase; chlorophyllase; shoots
Abstract:
... Chlorophyll (Chl) degradation was found to be related to the endogenous gibberellin (GA) content in shoots during senescence in the perennial plant Paris polyphylla var. yunnanensis (Franch.). Treatment with gibberellic acid (GA₃) significantly increased the content of endogenous GAs (GA₄ + GA₇), retarded the senescence of shoots, and the degradation of proteins and Chl. Chlorophyllase, Mg-dechela ...
DOI:
10.1007/s10535-010-0072-5

45. Hydrogen sulfide alleviated chromium toxicity in wheat

Author:
Zhang, H.; Hu, L. -Y.; Li, P.; Hu, K. -D.; Jiang, C. -X.; Luo, J. -P.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 743-747
ISSN:
0006-3134
Subject:
seed germination; peroxidase; dose response; seeds; catalase; ascorbate peroxidase; hydrogen peroxide; malondialdehyde; linoleate 13S-lipoxygenase; superoxide dismutase; wheat; Triticum aestivum; toxicity; chromium; hydrogen sulfide; esterases
Abstract:
... Effects of H₂S on seed germination under chromium (Cr) stress were investigated in wheat (Triticum aestivum L.). Under Cr stress, the percentage of germination of wheat seeds decreased, but this decrease could be alleviated by pretreatment with NaHS, an H₂S donor, in a dose-dependent manner. Furthermore, NaHS significantly enhanced the activities of amylase, esterase, superoxide dismutase, catalas ...
DOI:
10.1007/s10535-010-0133-9

46. Identification and transcriptional analysis of genes involved in Bacillus cereus-induced systemic resistance in Lilium

Author:
Liu, Y. -H.; Huang, C. -J.; Chen, C. -Y.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 697-702
ISSN:
0006-3134
Subject:
Bacillus cereus; Lilium formosanum; Northern blotting; disease severity; gene expression regulation; genes; leaf blight; leaves; photosystem II; sequence analysis; suppression subtractive hybridization; transcription (genetics)
Abstract:
... Bacillus cereus C1L was demonstrated to induce systemic resistance in Lilium formosanum against leaf blight caused by Botrytis elliptica. Suppression subtractive hybridization library of L. formosanum triggered by B. cereus C1L were screened and 3 differentially expressed genes were identified. Based on sequence analysis, these genes encoding putative glycine-rich protein, metallothionein-like pro ...
DOI:
10.1007/s10535-010-0123-y

47. In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants

Author:
Tyagi, P.; Khanduja, S.; Kothari, S.L.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 126-130
ISSN:
0006-3134
Subject:
Capparis decidua; benzyladenine; explants; indole acetic acid; indole butyric acid; leaves; micropropagation; naphthaleneacetic acid; random amplified polymorphic DNA technique; shoots
Abstract:
... A protocol for in vitro multiplication of Capparis decidua (Forsk.) Edgew. has been developed from cultured leaves procured from multiplying axillary shoots on the cultured nodal explants. The highest efficiency of shoot formation was observed on Murashige and Skoog (MS) medium containing 2 mg dm⁻³ benzyladenine (BA) and 0.5 mg dm⁻³ 1-naphthaleneacetic acid. The regenerated shoots were transferred ...
DOI:
10.1007/s10535-010-0019-x

48. In vitro propagation of Drosera intermedia in a single step

Author:
Grevenstuk, T.; Coelho, N.; Gonçalves, S.; Romano, A.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 391-394
ISSN:
0006-3134
Subject:
Drosera; growth regulators; kinetin; micropropagation; plantlets; rooting; seedlings
Abstract:
... A simple and efficient protocol for the micropropagation of Drosera intermedia, using cultures initiated from in vitro produced seedlings, is described. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher multiplication rates for ¼ MS (the lowest concentration), but was not affected by the addition of 0.1 mg dm⁻³ kinetin. In all ...
DOI:
10.1007/s10535-010-0071-6

49. Induction of capsaicinoid synthesis in Capsicum chinense cell cultures by salicylic acid or methyl jasmonate

Author:
Gutiérrez-Carbajal, M. G.; Monforte-González, M.; Miranda-Ham, M.de L.; Godoy-Hernández, G.; Vázquez-Flota, F.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 430-434
ISSN:
0006-3134
Subject:
Capsicum chinense; cell culture; elicitors; metabolism; methyl jasmonate; pepper; phenylalanine ammonia-lyase; salicylic acid; vanillin
Abstract:
... Suspension cultures of Habanero pepper (Capsicum chinense Jacq.) were exposed to salicylic acid or methyl jasmonate to change secondary metabolism. Both treatments led to the accumulation of capsaicinoids and their late biosynthetic intermediate, vanillin. Both elicitors had a positive effect on the activities of phenylalanine ammonia lyase and coumarate O-methyltransferase, but none of them repre ...
DOI:
10.1007/s10535-010-0078-z

50. Induction of tetraploidy in Juncus effusus by colchicine

Author:
Xu, L.; Najeeb, U.; Naeem, M. S.; Daud, M. K.; Cao, J. S.; Gong, H. J.; Shen, W. Q.; Zhou, W. J.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 659-663
ISSN:
0006-3134
Subject:
Juncus effusus; cultivars; intercellular spaces; diploidy; flow cytometry; tetraploidy; colchicine; stomata; mesophyll; chlorophyll; chloroplasts; vascular bundles
Abstract:
... Tetraploidy was induced in vitro in mat rush (Juncus effusus L.) cultivar Nonglin-4 by exposure to colchicine (0, 50, 100 and 500 mg dm⁻³) for 6, 12 and 24 h. Flow cytometric analysis was used to confirm the ploidy level. Anatomical and ultrastructural analyses at cellular and subcellular levels in tetraploid and diploid control plants revealed differences between diploid and tetraploid plants. Th ...
DOI:
10.1007/s10535-010-0117-9

51. Involvement of arabinogalactan proteins in the control of cell proliferation of Cucurbita pepo suspension cultures

Author:
Ben Amar, A.; Cobanov, P.; Ghorbel, A.; Mliki, A.; Reustle, G. M.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 321-324
ISSN:
0006-3134
Subject:
Cucurbita pepo; arabinogalactan proteins; arabinogalactans; callus; cell division; cell proliferation; cell suspension culture; chitinase; cultivars; culture media; growth promotion; immunoblotting; monoclonal antibodies; proteinases; zucchini
Abstract:
... Arabinogalactan proteins (AGPs) secreted by zucchini squash (Cucurbita pepo L.) cell cultures into the medium are implicated in cell proliferation. Conditioned medium derived from cell suspensions of squash cultivar Dundoo could enhance multiplication rate of slow-growing cell line Cx3005. To examine the role of AGPs, a precipitation assay was performed using Yariv reagent which binds selectively ...
DOI:
10.1007/s10535-010-0055-6

52. Is heterosis noticeable in the callus response of winter durum wheat F₁ hybrids

Author:
Ozbay, A.; Özgen, M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 769-772
ISSN:
0006-3134
Subject:
Triticum turgidum subsp. durum; callus; durum wheat; embryo culture; genotype; heterosis; hybrids; parents; soil; statistical analysis; vigor
Abstract:
... The effect of heterosis on callus induction, callus mass and number of regenerated plants from mature embryo cultures of winter durum wheat (Triticum durum Desf.) hybrids was studied. A total of 14 F₁ hybrids and their parents were used for mature embryo culture. The statistical analysis of the results revealed that positive heterosis in callus mass was determined in only one F₁ hybrid and in numb ...
DOI:
10.1007/s10535-010-0139-3

53. Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development

Author:
Wang, Y.; Zhang, Q. L.; Luo, Z. R.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 707-710
ISSN:
0006-3134
Subject:
Diospyros kaki; complementary DNA; fruiting; fruits; gene expression; genes; genotype; open reading frames; persimmons; rapid amplification of cDNA ends
Abstract:
... Leucoanthocyanidin reductase (LAR) converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon (Diospyros kaki Thunb.) fruits. A putative LAR gene (DkLAR) was isolated by rapid amplification of cDNA ends from young fruits. The full-length cDNA of DkLAR gene was 1 356 bp long and encoded an open reading frame of 349 residues. The deduced DkLAR protei ...
DOI:
10.1007/s10535-010-0125-9

54. Isolation and functional analysis of cDNAs similar to Hyp-1 involved in hypericin biosynthesis from Hypericum erectum

Author:
Jin, M. L.; Ahn, J. C.; Hwang, B.; Park, H. -S.; Lee, H. S.; Choi, D. -W.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 725-729
ISSN:
0006-3134
Subject:
polypeptides; callus; emodin; biosynthesis; complementary DNA; active ingredients; recombinant proteins; expressed sequence tags; flowers; amino acid sequences; metabolites; leaves; Hypericum perforatum; hypericin; sequence homology; Hypericum erectum
Abstract:
... Hypericin, a naphthodianthrone, has been identified as the principal active compound found in St. John's wort (Hypericum perforatum L.). To generate a gene resource for hypericin and other valuable metabolites, we generated expressed sequence tags (ESTs) from H. erectum. Analyses of the ESTs enabled us to select three cDNAs, HeHyp1, HeHyp2, and HeHyp3, evidencing significant sequence homology to H ...
DOI:
10.1007/s10535-010-0129-5

55. Isolation of resistance gene analogues to powdery mildew resistance sequences in hexaploid wheat

Author:
Feng, D. S.; Ma, X.; Lin, A. L.; Wang, H. G.; Tian, J. C.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 551-555
ISSN:
0006-3134
Subject:
stop codon; wheat; powdery mildew; amino acid sequences; Triticum aestivum; binding sites; hexaploidy; point mutation
Abstract:
... This paper reports the characterization of the powdery mildew resistance homologous genes family of Triticum aestivum. Using degenerate primer pair for wheat resistance genes, we have cloned seven 3′ truncated powdery mildew resistance gene homologous fragments Tpc5a, Tp25a, Tp25b, Tp3a5a, Tp3a5b, Tp4b5a and Tp4b5b. These fragments were sequenced. The deduced amino acid sequences showed that six o ...
DOI:
10.1007/s10535-010-0098-8

56. Leaf structural modifications in Populus × euramericana subjected to Zn excess

Author:
Di Baccio, D.; Minnocci, A.; Sebastiani, L.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 502-508
ISSN:
0006-3134
Subject:
intercellular spaces; zinc; photosynthesis; Populus; mesophyll; stomata
Abstract:
... In previous experiments elevated but sub-symptomatic applications of Zn (0.1 mM and 1 mM) caused impairments in growth parameters and photosynthetic performance of Populus × euramericana (Dode) Guinier clone I-214. The aim of this work was to evaluate leaf morphological and anatomical traits in this clone in response to the same Zn concentrations. The results showed that Zn treatments induced vari ...
DOI:
10.1007/s10535-010-0088-x

57. Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia

Author:
Jangid, R. K.; Sharma, R.; Sudarsan, Y.; Eapen, S.; Singh, G.; Purohit, A. K.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 703-706
ISSN:
0006-3134
Subject:
DNA; Vigna aconitifolia; callus; explants; gene expression; leaves; microwave treatment; mutagenesis; nucleotide sequences; polypeptides; random amplified polymorphic DNA technique; seedlings
Abstract:
... Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm⁻²) for 1, 3, 5 and 7 s before culturing. Callusing and shoot emergence from these explants were enhanced up to microwave exposure lasted 5 s while longer treatment of water-immersed explants delayed callusing. One polype ...
DOI:
10.1007/s10535-010-0124-x

58. Molecular cloning and characterization of a novel microsomal oleate desaturase gene DiFAD2 from Davidia involucrata Baill

Author:
Lei, N.; Peng, S.; Niu, B.; Chen, J.; Zhou, J.; Tang, L.; Xu, Y.; Wang, S.; Chen, F.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 41-46
ISSN:
0006-3134
Subject:
Davidia involucrata; amino acid sequences; amino acids; complementary DNA; fatty acid composition; genes; leaves; linoleic acid; molecular cloning; oleic acid; open reading frames; polyunsaturated fatty acids; reverse transcriptase polymerase chain reaction; sequence analysis; stems; temperature; tissues
Abstract:
... In the conversion of oleic acid to linoleic acid, δ¹²-fatty acid desaturase (δ¹²-FAD) is involved. Based on the conserved oligo amino acid residues of the FAD2 genes from other plants, a new full-length cDNA (DiFAD2) encoding a δ¹²-FAD was cloned from Davidia involucrata Baill. Sequence analysis indicated that the DiFAD2 gene had an open reading frame (ORF) of 1 149 bp, coding for 382 amino acids ...
DOI:
10.1007/s10535-010-0006-2

59. Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris

Author:
Subotić, A.; Trifunović, M.; Jevremović, S.; Petrić, M.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 592-596
ISSN:
0006-3134
Subject:
2,4-D; cell division; developmental stages; endangered species; leaves; plant growth substances; scanning electron microscopy; shoots; somatic embryogenesis; somatic embryos
Abstract:
... Direct somatic embryogenesis of Frittilaria meleagris L. was induced using leaf base explants excised from in vitro grown shoots. Somatic embryos occurred at the basal part of leaf explants 4 weeks after culture on a Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or kinetin (KIN). The highest number of somatic embryos (SEs) were f ...
DOI:
10.1007/s10535-010-0107-y

60. Morphological and anatomical observations of abnormal somatic embryos from anther cultures of Citrus reticulata

Author:
Benelli, C.; Germanà, M. A.; Ganino, T.; Beghe', D.; Fabbri, A.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 224-230
ISSN:
0006-3134
Subject:
Citrus reticulata; abscission; anther culture; developmental stages; leaves; mandarins; morphogenesis; plantlets; somatic embryogenesis; somatic embryos; stems
Abstract:
... A morphological and anatomical study of regenerants obtained from mandarin anther culture was carried out. Beside morphologically normal somatic embryos, abnormal structures were originated in the course of somatic embryogenesis. Anatomical anomalies can be found at several growth stages, causing the formation of slender stems, stubby structures, non-functional leaves. When too long, some structur ...
DOI:
10.1007/s10535-010-0040-0

61. Optimization of Algerian fir somatic embryos maturation

Author:
Vooková, B.; Machava, J.; Šalgovičová, A.; Kormuťák, A.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 177-180
ISSN:
0006-3134
Subject:
Abies numidica; chlorophyll; cotyledons; growth regulators; seedlings; somatic embryos
Abstract:
... The effect of maturation pretreatment on development and growth of Abies numidica De Lann. somatic embryos was studied. The most beneficial was pre-culturing on Schenk and Hildebrandt medium without growth regulator for 2 weeks. Dry mass accumulation of emblings was lower than that of seedlings after 50 d of culturing. Contents of microelements in seedlings were higher than in emblings, but macroe ...
DOI:
10.1007/s10535-010-0030-2

62. Organogenesis and Agrobacterium tumefaciens-mediated transformation of Eucalyptus saligna with P5CS gene

Author:
Dibax, R.; Deschamps, C.; Bespalhok Filho, J. C.; Vieira, L. G. E.; Molinari, H. B. C.; De Campos, M. K. F.; Quoirin, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 6-12
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Eucalyptus saligna; Southern blotting; biosynthesis; callus; callus formation; cefotaxime; coculture; explants; genetic transformation; genotype; greenhouses; in vitro regeneration; kanamycin; leaves; organogenesis; polymerase chain reaction; proline; rooting; roots; shoots; transgenes
Abstract:
... The purpose of this research was Eucalyptus saligna in vitro regeneration and transformation with P5CSF129A gene, which encodes Δ¹-pyrroline-5-carboxylate synthetase (P5CS), the key enzyme in proline biosynthesis. After selection of the most responsive genotype, shoot organogenesis was induced on leaf explants cultured on a callus induction medium (CI) followed by subculture on a shoot induction m ...
DOI:
10.1007/s10535-010-0002-6

63. Over-expression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance

Author:
Xue, Y.; Peng, R.; Xiong, A.; Li, X.; Zha, D.; Yao, Q.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 105-111
ISSN:
0006-3134
Subject:
Arabidopsis thaliana; Saccharomyces cerevisiae; biochemical pathways; chlorophyll; electrical conductivity; fluorescence; gene overexpression; genes; heat shock proteins; heat stress; heat tolerance; malondialdehyde; proline; sugars; temperature; transgenic plants; yeasts
Abstract:
... In the yeast Saccharomyces cerevisiae, the molecular chaperone HSP26 has the remarkable ability to sense increases in temperature directly and can switch from an inactive to a chaperone-active state. In this report, we analyzed the effect of expression of HSP26 in Arabidopsis thaliana plants and their response to high temperature stress. The hsp26 transgenic plants exhibited stronger growth than w ...
DOI:
10.1007/s10535-010-0015-1

64. Photosynthesis, respiration and antioxidant enzymes in pepper leaves under drought and heat stresses

Author:
Hu, W. H.; Xiao, Y. A.; Zeng, J. J.; Hu, X. H.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 761-765
ISSN:
0006-3134
Subject:
ascorbate peroxidase; gas exchange; drought tolerance; cytosol; drought; Capsicum annuum; fluorescence; hydrogen peroxide; heat stress; mitochondria; superoxide anion; leaves; cultivars; photorespiration; superoxide dismutase; heat; enzyme activity; chlorophyll; pepper; chloroplasts
Abstract:
... Leaf gas exchange, chlorophyll fluorescence and activities of antioxidant enzymes were studied in two pepper (Capsicum annuum L.) cultivars grown under drought (D) and heat (H), as well as under drought and heat in combination (HD). The drought-tolerant cv. Zhengjiao 13 exhibited greater net photosynthetic rate (PN) and cytochrome respiratory pathway activity (RSHAM), and lower contents of superox ...
DOI:
10.1007/s10535-010-0137-5

65. Photosynthetic performance and acclimation of Incarvillea delavayi to water stress

Author:
Cai, Y. -F.; Zhang, S. -B.; Hu, H.; Li, S. -Y.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 89-96
ISSN:
0006-3134
Subject:
carbon dioxide; nitrogen; fluorescence; electron transfer; acclimation; water stress; soil water; Incarvillea delavayi; field capacity; photosynthesis; mesophyll; chlorophyll; stomata
Abstract:
... The photosynthetic performance and related leaf traits of Incarvillea delavayi Bur. et Franch were studied at different water regimes to assess its capacity for photosynthetic acclimation to water stress. The initial response of I. delavayi to water stress was the closure of stomata, which resulted in down-regulation of photosynthesis. The stomatal limitation (SL) represented the main component to ...
DOI:
10.1007/s10535-010-0013-3

66. Photosystems activities and polypeptide composition of Cyamopsis tetragonoloba and Vigna mungo thylakoids as affected by exclusion of solar UV radiation

Author:
Amudha, P.; Jayakumar, M.; Kulandaivelu, G.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 339-343
ISSN:
0006-3134
Subject:
Cyamopsis tetragonoloba; Vigna mungo; black gram; photosystem I; photosystem II; polypeptides; protein composition; proteins; thylakoids; ultraviolet radiation
Abstract:
... The impacts of solar UV (280-400 nm) radiation on photosynthetic activities and polypeptide composition of thylakoids of cluster bean (Cyamopsis tetragonoloba L, UV-B sensitive) and black gram (Vigna mungo L., UV-B resistant) plants were compared. The activity of photosystem 1 and especially photosystem 2 increased in cluster bean while decreased in black gram, when either UV-B or UV-B + UV-A radi ...
DOI:
10.1007/s10535-010-0059-2

67. Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants

Author:
Das, A.; Kesari, V.; Rangan, L.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 423-429
ISSN:
0006-3134
Subject:
Curcuma; acetic acid; adventitious shoots; agar; benzyladenine; genetic stability; greenhouses; indole butyric acid; maltose; micropropagation; naphthaleneacetic acid; plantlets; random amplified polymorphic DNA technique; rhizomes; roots; spring
Abstract:
... An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 μM benzyladenine and 2.68 μM α-naphthalene ac ...
DOI:
10.1007/s10535-010-0077-0

68. Production and selection of marker-free transgenic plants of Petunia hybrida using site-specific recombination

Author:
Khan, R. S.; Nakamura, I.; Mii, M.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 265-271
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Petunia hybrida; Southern blotting; adventitious shoots; antibiotics; beta-glucuronidase; excision; explants; genes; genetic markers; phenotype; plant growth substances; polymerase chain reaction; site-specific recombination; transgenic plants
Abstract:
... MAT (multi-auto-transformation) vector system has been one of the strategies to excise the selection marker gene from transgenic plants. Agrobacterium tumefaciens strain EHA105 harboring an ipt-type MAT vector, pNPI132, was used to produce morphologically normal transgenic Petunia hybrida ‘Dainty Lady' employing isopentenyl transferase (ipt) gene as the selection marker gene. β-glucuronidase (GUS) ...
DOI:
10.1007/s10535-010-0046-7

69. Proline accumulation and the expression of Δ¹-pyrroline-5-carboxylate synthetase in two safflower cultivars

Author:
Thippeswamy, M.; Chandraobulreddy, P.; Sinilal, B.; Shiva Kumar, M.; Sudhakar, Chinta
Source:
Biologia plantarum 2010 v.54 no.2 pp. 386-390
ISSN:
0006-3134
Subject:
Carthamus tinctorius; biochemical pathways; chlorophyll; cultivars; drought tolerance; enzymes; gene expression regulation; genes; leaves; proline; reverse transcriptase polymerase chain reaction; water stress
Abstract:
... Proline (Pro) accumulation under water stress was measured in safflower (Carthamus tinctorius L.) drought tolerant cv. A1 and sensitive cv. Nira. Activities of pyrroline-5-carboxylate reductase (P5C reductase) and pyrroline-5-carboxylate synthetase (P5C synthetase), two enzymes involved in the Pro biosynthetic pathway were also estimated. Water stress resulted in a reduction in the leaf dry mass a ...
DOI:
10.1007/s10535-010-0070-7

70. Response of grape rootstocks to salinity: changes in root growth, polyamines and abscisic acid

Author:
Upreti, K. K.; Murti, G. S. R.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 730-734
ISSN:
0006-3134
Subject:
abscisic acid; electrolytes; grapes; osmotic pressure; potassium; putrescine; root growth; rootstocks; salinity; sodium; sodium chloride; spermidine; spermine; streams
Abstract:
... Effects of salinity (0, 50, 100 and 250 mM NaCl) on growth, root:shoot dry mass ratio, osmotic potential (ψx), electrolyte leakage and contents of Na⁺ and K⁺, polyamines and abscisic acid (ABA) were studied in the grape rootstocks Dogridge, 1613, St. George and Salt Creek. In control rootstocks, the root length was highest in Dogridge and contents of K⁺ and ABA in Salt Creek. Salinity treatments i ...
DOI:
10.1007/s10535-010-0130-z

71. Role of calcium in nitric oxide-induced programmed cell death in tobacco protoplasts

Author:
Wang, Y.; Lin, J. S.; Wang, G. X.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 471-476
ISSN:
0006-3134
Subject:
apoptosis; calcium; calcium channels; chelating agents; ethylene glycol tetraacetic acid; membrane potential; mitochondria; mitochondrial membrane; nitric oxide; nitroprusside; permeability; protoplasts; tobacco
Abstract:
... We tried to determine the mechanisms by which Ca²⁺ mediated NO-induced programmed cell death (PCD) in tobacco protoplasts. Treatment of tobacco protoplasts with the NO donor sodium nitroprusside (SNP) resulted in a rapid [Ca²⁺]cyt accumulation and decrease in mitochondrial membrane potential (ΔΨm) before the appearance of PCD. NO-induced PCD could be largely prevented not only by NO scavenger c-PT ...
DOI:
10.1007/s10535-010-0083-2

72. Salicylic acid increased aldose reductase activity and sorbitol accumulation in tomato plants under salt stress

Author:
Tari, I.; Kiss, G.; Deér, A. K.; Csiszár, J.; Erdei, L.; Gallé, Á.; Gémes, K.; Horváth, F.; Poór, P.; Szepesi, Á.; Simon, L. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 677-683
ISSN:
0006-3134
Subject:
aldehyde reductase; ammonium sulfate; cellulose; culture media; enzyme activity; glucose; glucose 6-phosphate; isozymes; leaf extracts; leaves; roots; salicylic acid; salt stress; sodium chloride; sorbitol; stress tolerance; tissues; tomatoes
Abstract:
... Increased aldose reductase (ALR) activities were detected in the leaf tissues of tomato plants grown for 3 weeks in culture medium containing 10⁻⁷ or 10⁻⁴ M salicylic acid (SA), and in the roots after the 10⁻⁴ M SA pretreatment. The ALR activity changed in parallel with the sorbitol content in the leaves of the SA-treated plants. Salt stress elicited by 100 mM NaCl enhanced the accumulation of sor ...
DOI:
10.1007/s10535-010-0120-1

73. Sensitivity of stem and petiole hydraulic conductance of deciduous trees to xylem sap ion concentration

Author:
Aasamaa, K.; Sõber, A.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 299-307
ISSN:
0006-3134
Subject:
abscisic acid; correlation; developmental stages; leaf conductance; leaf water potential; mercuric chloride; petioles; sap; stomatal conductance; stomatal movement; summer; trees; xylem
Abstract:
... Hydraulic conductance of stem and petioles increased in response to an increase in xylem sap ion concentration, and decreased in response to a decrease in the ion concentration in six temperate deciduous tree species. The ion sensitivity of hydraulic conductance of stem and petioles was higher than the ion sensitivity of the stem alone. The ion sensitivity was lowest in the earliest developmental ...
DOI:
10.1007/s10535-010-0052-9

74. Somatic embryogenesis and regeneration of Vigna radiata

Author:
Sivakumar, P.; Gnanam, R.; Ramakrishnan, K.; Manickam, A.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 245-251
ISSN:
0006-3134
Subject:
2,4-D; Vigna radiata; abscisic acid; benzyladenine; callus; cell suspension culture; explants; fruiting; glutamine; indole butyric acid; leaves; maltose; mannitol; mung beans; plantlets; polyethylene glycol; potassium nitrate; proline; roots; somatic embryogenesis; somatic embryos; sucrose; vitamins
Abstract:
... An efficient regeneration protocol via somatic embryogenesis was optimized for mung bean [Vigna radiata (L.) Wilczek; cv. Vamban 1]. Primary leaf explants were used for embryogenic callus induction in MMS medium (Murashige and Skoog salts with B5 vitamins) containing 2.0 mg dm⁻³ 2,4-dichlorophenoxyacetic acid (2,4-D), 150 mg dm⁻³ glutamine and 3 % sucrose. Fast growing, highly embryogenic cell sus ...
DOI:
10.1007/s10535-010-0043-x

75. Somatic embryogenesis from rhizome explants of Cymbopogon winterianus

Author:
Dey, T.; Bhattacharya, S.; Ghosh, P. D.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 325-328
ISSN:
0006-3134
Subject:
2,4-D; Cymbopogon winterianus; biotin; calcium; callus; coconut water; culture media; explants; kinetin; plantlets; rhizomes; somatic embryogenesis
Abstract:
... Plantlet regeneration through indirect somatic embryogenesis was attempted from rhizome derived callus of Cymbopogon winterianus Jowitt (cv. Jorlab2). Optimum callus was induced on Murashige and Skoog (MS) basal medium supplemented with 4 mg dm⁻³ 2,4-dichlorophenoxyacetic acid (2,4-D). Initially the callus was friable, shiny white and watery in nature. After subculturing on MS medium containing 2, ...
DOI:
10.1007/s10535-010-0056-5

76. Spatial and temporal dynamics of peroxidase and amine oxidase activity is linked to polyamines and lignin in wheat grains

Author:
Asthir, B.; Kaur, S.; Spoor, W.; Roitsch, T.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 525-529
ISSN:
0006-3134
Subject:
Triticum aestivum; cell walls; cultivars; diamine oxidase; flowering; heat; heat tolerance; lignification; lignin; peroxidase; polyamines; temporal variation; testa; wheat
Abstract:
... The regulation of contents and activities of peroxidase (POX), diamine oxidase (DAO) and polyamine oxidase (PAO) were determined in relation to polyamines and lignin content in wheat (Triticum aestivum L.) grains. Two cultivars WH 542 (heat susceptible) and PBW 343 (heat tolerant) were used. Activities of POX, DAO and PAO were substantially higher in PBW 343 as compared with WH 542 and appeared to ...
DOI:
10.1007/s10535-010-0092-1

77. Stem respiration of Norway spruce trees under elevated CO₂ concentration

Author:
Acosta, M.; Pokorný, R.; Janouš, D.; Marek, M. V.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 773-776
ISSN:
0006-3134
Subject:
Picea abies; carbon dioxide; growing season; respiratory rate; seasonal variation; temperature; tree growth; trees
Abstract:
... Measurements of stem respiration were conducted for a period of four years (1999-2002) in 14-year old Norway spruce (Picea abies [L.] Karst) trees exposed to ambient (CA) and elevated CO₂ concentration (CE; ambient plus 350 μmol mol⁻¹). Stem respiration measurements of six trees per treatment were carried out 2-3 times per month during the growing season. Stem respiration in CE treatment was highe ...
DOI:
10.1007/s10535-010-0140-x

78. Stimulation of antioxidative enzymes and polyamines during stripe rust disease of wheat

Author:
Asthir, B.; Koundal, A.; Bains, N. S.; Mann, S. K.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 329-333
ISSN:
0006-3134
Subject:
Puccinia striiformis; Triticum aestivum; catalase; cell walls; chlorophyll; cultivars; diamine oxidase; enzyme activity; lignin; peroxidase; putrescine; spermidine; spermine; stripe rust; superoxide dismutase; wheat
Abstract:
... Content of polyamines and activities of antioxidative enzymes in response to stripe rust disease caused by Puccinia striiformis have been studied in two wheat (Triticum aestivum L.) cultivars PBW 343 (resistant) and HD 2329 (susceptible). Various infection stages ranging from traces to 100 % were collected. Infection leads to stimulation of peroxidase (POD), superoxide dismutase (SOD), catalase, d ...
DOI:
10.1007/s10535-010-0057-4

79. Transformation of chickpea: effect of genotype, explant, Agrobacterium-strain and composition of culture medium

Author:
Bhattacharjee, B.; Mohan, M.; Nair, S.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 21-32
ISSN:
0006-3134
Subject:
Agrobacterium; Cicer arietinum; beta-glucuronidase; callus; chickpeas; culture media; genes; genotype; indole butyric acid; rooting; transgenic plants
Abstract:
... Reproducible and high-frequency transgenic plant regeneration from callus and embryo axes of four different genotypes of chickpea (Cicer arietinum) was achieved after Agrobacterium-mediated transformation. Three different strains of Agrobacterium (EHA105, AGL1 and LBA4404) harboring the binary vector pCAMBIA1301 containing β-glucuronidase (GUS) and hygromycin phosphotransferase (hpt) genes under t ...
DOI:
10.1007/s10535-010-0004-4

80. Transformation of potato via Agrobacterium coated microparticle bombardment

Author:
Nguyen, T. T.; Dix, P. J.; Nugent, G. D.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 141-144
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Solanum tuberosum; biolistics; genes; genetically modified organisms; gold; leaves; plasmids; potatoes; shoots; transfer DNA
Abstract:
... The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three ...
DOI:
10.1007/s10535-010-0022-2

81. Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector

Author:
Šmídková, M.; Müller, M.; Thönes, N.; Piuko, K.; Angelisová, P.; Velemínský, J.; Angelis, K. J.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 451-460
ISSN:
0006-3134
Subject:
adjuvants; Nicotiana benthamiana; Tobacco rattle virus; insects; tomatoes; alkaloids; antibodies; Agrobacterium radiobacter; cell-mediated immunity; cytosine; enzyme-linked immunosorbent assay; guanine; viruses; Nicotiana tabacum; leaves; coat proteins; Human papillomavirus type 16; host range; humans; monitoring; electron microscopy; mice; immunization; toxicity; tobacco; Solanum lycopersicum var. lycopersicum; neutralization; freeze drying; virus-like particles
Abstract:
... The major capsid protein L1 of human papillomavirus type 16 (HPV16) was transiently expressed in tobacco (Nicotiana benthamiana and Nicotiana tabacum) and tomato (Lycopersicon esculentum) leaves using Agrobacterium tumefaciens. The expression vector pTV00 was derived from tobacco rattle virus (TRV). The highest L1 expression 15 μg g⁻¹(f.m.) was achieved when the coding sequence of L1 was optimized ...
DOI:
10.1007/s10535-010-0081-4

82. Two enzymatic sources of nitric oxide in different organs of apple plant

Author:
Gao, H. J.; Yang, H. Q.; Wang, J. Y.; Wang, J. X.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 789-792
ISSN:
0006-3134
Subject:
Malus domestica; NAD (coenzyme); apples; developmental stages; leaves; mitochondria; nitric oxide; nitric oxide synthase; nitrites; roots; stems; trees
Abstract:
... Nitric oxide production, nitric oxide synthase (NOS) and mitochondrial nitrite-reducing activities in roots, leaves and stems of different developmental stages were investigated, using potted 3-year-old apple (Malus domestica Borkh.) trees. The arginine-dependent NOS activity is sensitive to NOS inhibitor L-NAME and aminoguanidine (AG), with L-NAME being more effective than AG. Endogenous NO produ ...
DOI:
10.1007/s10535-010-0144-6

83. Using morphological diagnosis and molecular markers to assess the clonal fidelity of micropropagated Echinacea purpurea regenerants

Author:
Chuang, S. J.; Chen, C. L.; Chen, J. J.; Sung, J. M.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 539-542
ISSN:
0006-3134
Subject:
DNA; Echinacea purpurea; amplified fragment length polymorphism; explants; genetic markers; leaves; micropropagation
Abstract:
... Both morphological characteristics and amplified fragment length polymorphism (AFLP) markers were used to validate the genetic fidelity of 1 080 field-grown Echinacea purpurea plants regenerated from leaf explants of donor T5-9. Morphological diagnosis revealed that 1 067 out of 1 080 regenerants were normal, while 13 regenerants were aberrant. AFLP analysis was further performed to assess DNA var ...
DOI:
10.1007/s10535-010-0095-y

84. Zinc and cadmium effects on growth and ion distribution in Populus tremula × Populus alba

Author:
Durand, T. C.; Hausman, J. F.; Carpin, S.; Alberic, P.; Baillif, P.; Label, P.; Morabito, D.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 191-194
ISSN:
0006-3134
Subject:
Populus alba; Populus tremula; cadmium; calcium; genotype; growth retardation; iron; leaves; magnesium; photosynthesis; potassium; soil; zinc
Abstract:
... The effects of cadmium and zinc on growth and mineral distribution in Populus tremula × P. alba genotype 717-1B4 were investigated. Exposure to 360 mg(Cd) kg⁻¹(soil) resulted in accumulation of Cd in all organs and inhibition of primary and secondary growth as well as of the net photosynthetic rate. No growth inhibition occurred under zinc exposure. Cd was mainly stored in the woody parts of stem, ...
DOI:
10.1007/s10535-010-0033-z

85. An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers

Author:
Samantaray, S.; Maiti, S.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 334-338
ISSN:
0006-3134
Subject:
Chlorophytum borivilianum; DNA; adenine; benzyladenine; explants; genetic stability; greenhouses; indole butyric acid; micropropagation; naphthaleneacetic acid; plantlets; random amplified polymorphic DNA technique; rooting; shoots; soil; sulfates
Abstract:
... Rapid micropropagation was achieved in Chlorophytum borivilianum Santapau and Fernandes using shoot base as explants. Multiple shoots were induced on Murashige and Skoog's (MS) medium supplemented with 3.0 mg dm⁻³ 6-benzylaminopurine, 0.1 mg dm⁻³ 1-naphthaleneacetic acid, 150 mg dm⁻³ adenine sulphates and 3 % saccharose. Rooting was readily achieved upon transferring the shoots onto half strength ...
DOI:
10.1007/s10535-010-0058-3

86. The effect of 5-azacytidine on wheat seedlings responses to NaCl stress

Author:
Zhong, L.; Xu, Y. H.; Wang, J. B.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 753-756
ISSN:
0006-3134
Subject:
Triticum aestivum; biomass production; catalase; cultivars; malondialdehyde; methylation; peroxidase; roots; salinity; salt stress; salt tolerance; seedlings; sodium chloride; superoxide dismutase; tissues; wheat
Abstract:
... The effect of 5-azacytidine (5-azaC) on the alleviation of damaging effects of NaCl treatment was studied in two wheat (Triticum aestivum L.) cultivars differing in salt tolerance (salt-tolerant Dekang-961 and sensitive Lumai-15). The plants were pre-treated or not with 50 μM 5-azaC and then subjected to salt stress induced by 100 or 150 mM NaCl. Salinity caused reduction in biomass accumulation a ...
DOI:
10.1007/s10535-010-0135-7

87. The effects of brassinosteroids on photosynthetic parameters in leaves of two field-grown maize inbred lines and their F1 hybrid

Author:
Kočová, M.; Rothová, O.; Holá, D.; Kvasnica, M.; Kohout, L.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 785-788
ISSN:
0006-3134
Subject:
aqueous solutions; brassinosteroids; carotenoids; chlorophyll; corn; electron transfer; foliar spraying; hybrids; inbred lines; leaves; photosynthesis; pigments
Abstract:
... The effect of foliar spray with 10⁻¹² M aqueous solutions of 24-epibrassinolide or a synthetic androstane analogue of castasterone on the activity of photosystem (PS) 1, the Hill reaction activity, the content of photosynthetic pigments and the specific leaf mass was examined for three different leaves developed after brassinosteroid (BR) treatment in two inbred lines of field-grown maize and thei ...
DOI:
10.1007/s10535-010-0143-7

88. The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa

Author:
Novero, A. U.; Ford, R.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 579-582
ISSN:
0006-3134
Subject:
Vicia sativa; biochemical pathways; cysteine; genes; metabolism; sulfates; sulfur; thiols
Abstract:
... The o-acetylserine (thiol) lyase (OAS-TL) gene is part of the plant sulfate assimilation pathway. The synthesis of cysteine, the first product of the pathway containing organic sulfur, has been previously observed to be dependent on the availability of sulfur and activity of OAS-TL. In this study, the ability of Vicia sativa L. to alter its metabolism to compensate for deficiencies brought about b ...
DOI:
10.1007/s10535-010-0104-1

89. A low-noise multi-channel device for the monitoring of systemic electrical signal propagation in plants

Author:
Ilík, P.; Hlaváčková, V.; Krchňák, P.; Nauš, J.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 185-190
ISSN:
0006-3134
Subject:
action potentials; electrocardiography; electrodes; gels; monitoring; physiological response; tomatoes
Abstract:
... Long-distance electrical signals generated in locally stimulated plants are linked with systemic physiological responses. The propagation of electrical signal through a plant can be measured by multiple electrodes attached to different sites of a plant body. As this signal has to be measured with the sensitivity of tens of microvolts, it can be easily disturbed by power-line hums or external elect ...
DOI:
10.1007/s10535-010-0032-0

90. ABA or cadmium induced phytochelatin synthesis in potato tubers

Author:
Stroiński, A.; Chadzinikolau, T.; Giżewska, K.; Zielezińska, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 117-120
ISSN:
0006-3134
Subject:
Solanum tuberosum; abscisic acid; cadmium; cysteine; glutathione; potatoes; tissues; tubers
Abstract:
... Short-term treatment of potato (Solanum tuberosum L.) tuber discs with CdCl₂ solution elevated both the StPCS1 transcript level, phytochelatin synthase (PCS) activity and contents of phytochelatin (PC) and abscisic acid (ABA). Similar effects but less marked were noticed after treatment of tuber tissues with ABA solution. Cd-treatment increased also cysteine (CYS) content but did not change glutat ...
DOI:
10.1007/s10535-010-0017-z

91. Alteration in growth and thylakoid membrane lipid composition of Azolla caroliniana under phosphate deficiency

Author:
Ismail, G. S. M.; Mohamed, H. E.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 671-676
ISSN:
0006-3134
Subject:
Azolla caroliniana; anthocyanins; biomass production; chlorophyll; fatty acid composition; ferns and fern allies; growth retardation; phosphates; phospholipids; phosphorus; photosystem II; thylakoids; ultrastructure
Abstract:
... The changes in the fresh biomass accumulation, photosynthetic and anthocyanin pigments, photosystem 2 (PS 2) activity, ultrastructure of chloroplast, total lipids and fatty acid composition of thylakoid membrane were followed in the aquatic fern Azolla caroliniana grown on medium either deficient or supplied with various phosphorus concentrations. The content of photosynthetic pigments and the ant ...
DOI:
10.1007/s10535-010-0119-7

92. Antioxidative enzymatic protection in leaves of two contrasting cowpea cultivars under salinity

Author:
Maia, J. M.; Costa de Macedo, C. E.; Voigt, E. L.; Freitas, J. B. S.; Silveira, J. A. G.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 159-163
ISSN:
0006-3134
Subject:
Vigna unguiculata; antioxidants; ascorbate peroxidase; cowpeas; cultivars; electrolytes; leaves; peroxidase; phenol; salinity; salt stress; salt tolerance; sodium; sodium chloride; superoxide dismutase; water content
Abstract:
... The aim of this work was to investigate the role of the antioxidant enzymes in salt tolerance comparing the salt-sensitive (Pérola) and a salt-tolerant (Pitiúba) cultivar of cowpea [Vigna unguiculata (L.) Walp.]. Salt stress (100 mM NaCl for 8 d) reduced the leaf growth rate more in the sensitive cultivar. The salt-induced decrease in the relative water content, Na⁺ accumulation and increase in le ...
DOI:
10.1007/s10535-010-0026-y

93. CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng

Author:
Kiselev, K. V.; Grishchenko, O. V.; Zhuravlev, Y. N.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 621-630
ISSN:
0006-3134
Subject:
Panax ginseng; cell culture; gene expression; genes; protein kinases; proteins; salt stress; salt tolerance; sodium chloride
Abstract:
... CDPKs (calcium-depended protein kinases) are of great importance for the activation of defense reactions in plants. In this study, we aimed to find a connection between CDPK expression and increased salt tolerance in Panax ginseng. Treatment of P. ginseng cell cultures with W7 (CDPK protein inhibitor) showed that CDPK proteins were necessary for salt tolerance. Expression of PgCDPK1c, PgCDPK2c and ...
DOI:
10.1007/s10535-010-0112-1

94. Changes in biomass and photosynthetic parameters of tomato plants exposed to trivalent and hexavalent chromium

Author:
Henriques, F. S.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 583-586
ISSN:
0006-3134
Subject:
biomass; chlorophyll; chromium; culture media; fluorescence; leaves; photosynthesis; plant growth; plant tissues; roots; stomatal conductance; tomatoes; toxicity
Abstract:
... Tomato plants were treated for two weeks with different concentrations of Cr(III) or Cr(VI) compounds to compare their toxic effects. The concentration of total Cr in plant tissues increased linearly with its concentration in the growth medium and Cr accumulated largely in the roots, regardless of the form in which it was supplied to the plant. All measured plant growth parameters were negatively ...
DOI:
10.1007/s10535-010-0105-0

95. Citrate transporters play an important role in regulating aluminum-induced citrate secretion in Glycine max

Author:
You, J. F.; Hou, N. N.; Xu, M. Y.; Zhang, H. M.; Yang, Z. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 766-768
ISSN:
0006-3134
Subject:
Glycine max; aluminum; citrates; cultivars; cycloheximide; exudation; mitochondrial membrane; plasma membrane; protein synthesis; protein synthesis inhibitors; pyridoxal; roots; secretion; soybeans; transporters
Abstract:
... To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor, anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated after 4 h of Al ...
DOI:
10.1007/s10535-010-0138-4

96. Clonal propagation of Zephyranthes grandiflora using bulbs as explants

Author:
Gangopadhyay, M.; Chakraborty, D.; Dewanjee, S.; Bhattacharya, S.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 793-797
ISSN:
0006-3134
Subject:
DNA; Zephyranthes; bulbs; carbendazim; explants; genomics; gibberellic acid; indole butyric acid; mercuric chloride; microbial contamination; micropropagation; microsatellite repeats; ornamental plants; polymerase chain reaction; roots; shoots; sucrose
Abstract:
... Zephyr lily (Zephyranthes grandiflora), an important ornamental plant has been micropropagated in vitro after controlling microbial contamination by a pretreatment with 0.2 % Bavistin and 0.1 % Pantomycin for 4 h before final sterilization with 0.1 % mercuric chloride. In 67 % of the sterile cultures, 11 shoots on average were regenerated directly from basal half of bulb scales in Murashige and Sk ...
DOI:
10.1007/s10535-010-0145-5

97. Cloning and characterization of two osmotin isoforms from Piper colubrinum

Author:
Mani, T.; Manjula, S.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 377-380
ISSN:
0006-3134
Subject:
Piper; antifungal properties; black pepper; breeding; cDNA libraries; complementary DNA; genes; point mutation
Abstract:
... In the present study, we report the cloning and sequence characterization of two isoforms of osmotin, an antifungal PR-5 gene homologue, from a salicylic acid-induced subtracted cDNA library earlier generated in Piper colubrinum. The larger form of the gene is 693 bp long, encoding a 21.5 kDa protein. The smaller form comprises a 543 bp long coding sequence which code for a protein of 16.4 kDa. A ...
DOI:
10.1007/s10535-010-0068-1

98. Contents of polyamines during vernalization in wheat and the effect of zearalenone

Author:
Filek, M.; Biesaga-Kościelniak, J.; Marcińska, I.; Cvikrová, M.; Macháčková, I.; Krekule, J.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 483-487
ISSN:
0006-3134
Subject:
cold treatment; germination; leaves; putrescine; roots; seedlings; spermidine; vernalization; winter wheat; zearalenone
Abstract:
... The contents of endogenous free and conjugated polyamines, putrescine (Put) and spermidine (Spd), were determined during 9 week of vernalization (at 5 °C) in winter wheat seedlings cultivated on Murashige and Skoog media without (MS0) and with 2 mg dm⁻³ zearalenone (MSZEN). At the 4th week of chilling treatment, which is sufficient to induce generative development in 30 % of plants, the marked inc ...
DOI:
10.1007/s10535-010-0085-0

99. Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Author:
Kumar, P.; Singhal, V. K.; Kaur, D.; Kaur, S.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 181-184
ISSN:
0006-3134
Subject:
Clematis; chromatin; chromosome number; meiosis; pollen; prophase; stickiness; tetraploidy; India
Abstract:
... Present cytological investigations from the cold desert regions of Lahaul-Spiti and Kinnaur (India) record the first ever tetraploid (2n=32) chromosome count and cytomixis in Clematis orientalis L. var. acutifolia Hook. f. et Thoms. The phenomenon of cytomixis (9.33-29.80 %) involving chromatin transfer among 2-3 proximate pollen mother cells (PMCs) during male meiosis occurs through narrow and br ...
DOI:
10.1007/s10535-010-0031-1

100. DIANTHIN, a negative selection marker in tobacco, is non-toxic in transgenic rice and confers sheath blight resistance

Author:
Shah, J. M.; Veluthambi, K.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 443-450
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Dianthus caryophyllus; Mungbean yellow mosaic virus; Oryza sativa; blight; callus; gene expression; genes; leaves; promoter regions; rice; tobacco; toxicity; transgenic plants
Abstract:
... The DIANTHIN gene encoding a ribosome-inactivating protein (RIP) from Dianthus caryophyllus L. was tested for negative selection in tobacco and rice. Tobacco leaf discs and scutellum-derived callus of rice were transformed with Agrobacterium tumefaciens strain LBA4404 (pSB1, pJAS1). pJAS1 harbors the DIANTHIN gene under the control of the CaMV 35S promoter. Tobacco transformation efficiency, in co ...
DOI:
10.1007/s10535-010-0080-5