You searched for:
Journal
Biologia plantarum
Remove constraint Journal: Biologia plantarum
Publication Year
2010
Remove constraint Publication Year: 2010
PubAg
Main content area
Limit your search
- 2010[remove]141
Search
141 Search Results
« Previous |
1 - 100 of 141
|
Next »
Search Results
- Author:
- Wiesner, I.; Wiesnerová, D.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 353-356
- ISSN:
- 0006-3134
- Subject:
- Begonia; DNA; alleles; genetic markers; genetic variation; germplasm; loci; nucleotide sequences; principal component analysis; sequence analysis
- Abstract:
- ... In this study, we wanted to inspect whether the evolutionary driven differences in primary sequences could correlate, and thus predict the genetic diversity of related marker loci, which is an important criterion to assess the quality of any DNA marker. We adopted new approach of quantitative symbolic DNA sequence analysis called DNA random walk representation to study multiallelic marker loci fro ...
- DOI:
- 10.1007/s10535-010-0062-7
- https://doi.org/10.1007/s10535-010-0062-7
- Author:
- Stroiński, A.; Chadzinikolau, T.; Giżewska, K.; Zielezińska, M.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 117-120
- ISSN:
- 0006-3134
- Subject:
- Solanum tuberosum; abscisic acid; cadmium; cysteine; glutathione; potatoes; tissues; tubers
- Abstract:
- ... Short-term treatment of potato (Solanum tuberosum L.) tuber discs with CdCl₂ solution elevated both the StPCS1 transcript level, phytochelatin synthase (PCS) activity and contents of phytochelatin (PC) and abscisic acid (ABA). Similar effects but less marked were noticed after treatment of tuber tissues with ABA solution. Cd-treatment increased also cysteine (CYS) content but did not change glutat ...
- DOI:
- 10.1007/s10535-010-0017-z
- https://doi.org/10.1007/s10535-010-0017-z
- Author:
- Daneshmand, F.; Arvin, M. J.; Kalantari, K. M.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 781-784
- ISSN:
- 0006-3134
- Subject:
- electrolytes; ascorbate peroxidase; lipid peroxidation; drought; polyethylene glycol; potatoes; aspirin; hydrogen peroxide; explants; sodium; peroxidase; osmotic stress; linoleate 13S-lipoxygenase; superoxide dismutase; physiological response; potassium; sodium chloride; adverse effects; glutathione-disulfide reductase; catalase; salinity; Solanum stoloniferum
- Abstract:
- ... The role of acetylsalicylic acid (0, 1 and 10 μM) pre-treatment in amelioration of salt and osmotic stress in a wild species of potato (Solanum stoloniferum) was investigated. We compared the effects of iso-osmotic concentrations of polyethylene glycol 6000 (15 %) and NaCl (80 mM) on the physiological responses of this species in explants grown in the liquid Murashige and Skoog medium. Both salt a ...
- DOI:
- 10.1007/s10535-010-0142-8
- https://doi.org/10.1007/s10535-010-0142-8
- Author:
- López, A. P.; Gochicoa, M. T. N.; Franco, A. R.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 349-352
- ISSN:
- 0006-3134
- Subject:
- catalase; enzyme activity; fruiting; fruits; peroxidase; ripening; strawberries; superoxide dismutase
- Abstract:
- ... The analyses of some antioxidant enzyme activities were carried out in the course of strawberry fruits development and ripening. The catalase activity was maximum in small-sized green fruits, it decreased in middle-sized green fruits and increased again during the ripening stages. The highest superoxide dismutase and peroxidase activities were observed in white fruits. ...
- DOI:
- 10.1007/s10535-010-0061-8
- https://doi.org/10.1007/s10535-010-0061-8
- Author:
- Vatankhah, E.; Niknam, V.; Ebrahimzadeh, H.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 509-514
- ISSN:
- 0006-3134
- Subject:
- Crocus sativus; callus; catalase; catechol oxidase; enzyme activity; kinetin; naphthaleneacetic acid; organogenesis; peroxidase; protein content; roots; shoots; sprouting; superoxide dismutase; thidiazuron
- Abstract:
- ... The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm⁻³ 1-naphthaleneacetic acid (NAA) + 1.0 mg dm⁻³ thidiazuron and 1.0 mg dm⁻³ NAA + 2.0 mg dm⁻³ kinetin. For sprouting, regenerated shoot were subcultured on Mu ...
- DOI:
- 10.1007/s10535-010-0089-9
- https://doi.org/10.1007/s10535-010-0089-9
- Author:
- Abenavoli, M. R.; Lupini, A.; Oliva, S.; Sorgonà, A.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 149-153
- ISSN:
- 0006-3134
- Subject:
- H-transporting ATP synthase; caffeic acid; corn; hydroponics; p-coumaric acid; plasma membrane; potassium nitrate; seedlings; umbelliferones
- Abstract:
- ... Seven-day-old maize seedlings grown in a nitrogen-free hydroponic culture were exposed for 48 h to 0, 100 and 300 μM trans-cinnamic, p-coumaric, ferulic, caffeic acids, umbelliferone and 200 μM KNO₃. Net nitrate uptake was affected by trans-cinnamic, ferulic and p-coumaric acids in a concentration-dependent manner, and trans-cinnamic acid appeared to be the strongest inhibitor. Conversely, at low ...
- DOI:
- 10.1007/s10535-010-0024-0
- https://doi.org/10.1007/s10535-010-0024-0
- Author:
- Ismail, G. S. M.; Mohamed, H. E.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 671-676
- ISSN:
- 0006-3134
- Subject:
- Azolla caroliniana; anthocyanins; biomass production; chlorophyll; fatty acid composition; ferns and fern allies; growth retardation; phosphates; phospholipids; phosphorus; photosystem II; thylakoids; ultrastructure
- Abstract:
- ... The changes in the fresh biomass accumulation, photosynthetic and anthocyanin pigments, photosystem 2 (PS 2) activity, ultrastructure of chloroplast, total lipids and fatty acid composition of thylakoid membrane were followed in the aquatic fern Azolla caroliniana grown on medium either deficient or supplied with various phosphorus concentrations. The content of photosynthetic pigments and the ant ...
- DOI:
- 10.1007/s10535-010-0119-7
- https://doi.org/10.1007/s10535-010-0119-7
- Author:
- Cherian, S.; Ferreira, R. B.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 587-591
- ISSN:
- 0006-3134
- Subject:
- Lupinus albus; gene expression regulation; granules; heat; heat shock proteins; heat shock response; heat stress; molecular weight; two-dimensional gel electrophoresis
- Abstract:
- ... An important aspect of heat-shock response of lupin (Lupinus albus cv. Rio Maior) is the formation of cytoplasmic granular aggregates, called heat-shock granules (HSGs). In this study, two-dimensional electrophoresis (2-DE) was used to detect the component proteins of HSG complexes formed in vivo. Evaluation of 2-DE revealed differential expression of several proteins under heat shock conditions w ...
- DOI:
- 10.1007/s10535-010-0106-z
- https://doi.org/10.1007/s10535-010-0106-z
- Author:
- Nautiyal, P. C.; Shono, M.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 715-719
- ISSN:
- 0006-3134
- Subject:
- Solanum lycopersicum var. lycopersicum; biomass; cell membranes; endoplasmic reticulum; genetically modified organisms; heat; heat shock proteins; heat stress; mitochondria; photosynthesis; protein synthesis; temperature; tomatoes
- Abstract:
- ... This communication examines the role of small heat shock proteins (sHsps) targeted to mitochondria (Mt) and endoplasmic reticulum (ER) in tomato plants (Lycopersicon esculentum Mill.) under heat stress. Genetic response of transgenic and wild type plants varied under optimum, moderately elevated and elevated temperature. In optimum temperature higher biomass was recorded in wild type than the tran ...
- DOI:
- 10.1007/s10535-010-0127-7
- https://doi.org/10.1007/s10535-010-0127-7
- Author:
- Maia, J. M.; Costa de Macedo, C. E.; Voigt, E. L.; Freitas, J. B. S.; Silveira, J. A. G.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 159-163
- ISSN:
- 0006-3134
- Subject:
- Vigna unguiculata; antioxidants; ascorbate peroxidase; cowpeas; cultivars; electrolytes; leaves; peroxidase; phenol; salinity; salt stress; salt tolerance; sodium; sodium chloride; superoxide dismutase; water content
- Abstract:
- ... The aim of this work was to investigate the role of the antioxidant enzymes in salt tolerance comparing the salt-sensitive (Pérola) and a salt-tolerant (Pitiúba) cultivar of cowpea [Vigna unguiculata (L.) Walp.]. Salt stress (100 mM NaCl for 8 d) reduced the leaf growth rate more in the sensitive cultivar. The salt-induced decrease in the relative water content, Na⁺ accumulation and increase in le ...
- DOI:
- 10.1007/s10535-010-0026-y
- https://doi.org/10.1007/s10535-010-0026-y
11. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
- Author:
- Vuletić, M.; Hadži-Tašković Šukalović, V.; Marković, K.; Dragišić Maksimović, J.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 530-534
- ISSN:
- 0006-3134
- Subject:
- Zea mays; ascorbate oxidase; ascorbate peroxidase; corn; enzyme activity; genotype; isoelectric focusing; nitrates; nitrogen; osmotic stress; peroxidase; phenolic compounds; polyethylene glycol; proline; roots; superoxide dismutase
- Abstract:
- ... The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO₃ ⁻) or both nitrogen forms, nitrate and ammonium (NH₄ ⁺/NO₃ ⁻). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline ...
- DOI:
- 10.1007/s10535-010-0093-0
- https://doi.org/10.1007/s10535-010-0093-0
- Author:
- Ma, K. -H.; Kim, K. -H.; Dixit, A.; Chung, I. -M.; Gwag, J. -G.; Kim, T. -S.; Park, Y. -J.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 272-278
- ISSN:
- 0006-3134
- Subject:
- Coix lacryma-jobi; algorithms; alleles; genetic background; genetic markers; genetic relationships; genetic variation; heterozygosity; loci; China; Korean Peninsula
- Abstract:
- ... The present study describes the assessment of genetic diversity and relationships among 79 Job's tears (Coix lacrymajobi L.) accessions collected from China and Korea using 17 microsatellite markers. A total of 57 alleles were detected with an average of 3.4 alleles per locus. A high frequency of rare alleles (36.3 %) was observed within the collection. Values for observed (HO), expected heterozyg ...
- DOI:
- 10.1007/s10535-010-0047-6
- https://doi.org/10.1007/s10535-010-0047-6
- Author:
- Kiselev, K. V.; Grishchenko, O. V.; Zhuravlev, Y. N.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 621-630
- ISSN:
- 0006-3134
- Subject:
- Panax ginseng; cell culture; gene expression; genes; protein kinases; proteins; salt stress; salt tolerance; sodium chloride
- Abstract:
- ... CDPKs (calcium-depended protein kinases) are of great importance for the activation of defense reactions in plants. In this study, we aimed to find a connection between CDPK expression and increased salt tolerance in Panax ginseng. Treatment of P. ginseng cell cultures with W7 (CDPK protein inhibitor) showed that CDPK proteins were necessary for salt tolerance. Expression of PgCDPK1c, PgCDPK2c and ...
- DOI:
- 10.1007/s10535-010-0112-1
- https://doi.org/10.1007/s10535-010-0112-1
- Author:
- Látalová, K.; Baláž, M.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 97-104
- ISSN:
- 0006-3134
- Subject:
- Orchidaceae; autotrophs; carbon; corn; mycelium; mycorrhizal fungi; nutrition; organic compounds; roots; saprotrophs; tubers
- Abstract:
- ... We studied the nutritional modes of the orchid Serapias strictiflora and its mycorrhizal fungus Epulorhiza sp. using the differences in carbon isotopic composition (δ¹³C) of C₃ orchid and C₄ maize tissues. We found that if cultivated in substrate lacking any organic compounds, the mycorrhizal extraradical mycelia (δ¹³C = −26.3 ± 0.2 ‰) developed well, despite being fully dependent on nutrition fro ...
- DOI:
- 10.1007/s10535-010-0014-2
- https://doi.org/10.1007/s10535-010-0014-2
- Author:
- Lu, J. Y.; Zhang, W. L.; Xue, H.; Pan, Y.; Zhang, C. H.; He, X. H.; Liu, M.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 112-116
- ISSN:
- 0006-3134
- Subject:
- DNA; DNA primers; Oryza sativa; amplified fragment length polymorphism; flight; genetic polymorphism; leaves; microsatellite repeats; rice; seeds; space flight
- Abstract:
- ... Differences of both amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) polymorphisms were compared between the 60-d-old rice (Oryza sativa L. cv. DH7) and F3 rice plants (SP3) derived from seed, which endured a 7-d-space flight in March 2002. Total leaf AFLP DNA bands amplified from 22 primer pairs were 537 in DH7, whereas 562 in SP3. From the total 267 SSR DNA bands ge ...
- DOI:
- 10.1007/s10535-010-0016-0
- https://doi.org/10.1007/s10535-010-0016-0
- Author:
- Henriques, F. S.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 583-586
- ISSN:
- 0006-3134
- Subject:
- biomass; chlorophyll; chromium; culture media; fluorescence; leaves; photosynthesis; plant growth; plant tissues; roots; stomatal conductance; tomatoes; toxicity
- Abstract:
- ... Tomato plants were treated for two weeks with different concentrations of Cr(III) or Cr(VI) compounds to compare their toxic effects. The concentration of total Cr in plant tissues increased linearly with its concentration in the growth medium and Cr accumulated largely in the roots, regardless of the form in which it was supplied to the plant. All measured plant growth parameters were negatively ...
- DOI:
- 10.1007/s10535-010-0105-0
- https://doi.org/10.1007/s10535-010-0105-0
- Author:
- Zhang, Y. H.; Chen, L. J.; He, J. L.; Qian, L. S.; Wu, L. Q.; Wang, R. F.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 164-168
- ISSN:
- 0006-3134
- Subject:
- Oryza sativa; ascorbate peroxidase; chlorophyll; cold tolerance; cooling; cultivars; fluorescence; genotype; glutathione-disulfide reductase; photosystem II; rice; superoxide dismutase
- Abstract:
- ... Characteristics of chlorophyll fluorescence and antioxidative system were investigated in rice (Oryza sativa L.) super-hybrid Liangyoupeijiu (LYPJ), maternal cultivar Peiai64s, and paternal cultivar indica rice 9311 under chilling stress. During 6-d chilling treatment, chlorophyll content of all three genotypes was gradually declined. However, the decrease in photosystem 2 (PS 2) maximum photochem ...
- DOI:
- 10.1007/s10535-010-0027-x
- https://doi.org/10.1007/s10535-010-0027-x
- Author:
- Weng, X. -Y.; Huang, Y. -Y.; Gao, H.; Sun, J. -Y.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 154-158
- ISSN:
- 0006-3134
- Subject:
- Aspergillus niger; Bacillus subtilis; Escherichia coli; Thermobifida fusca; Triticum aestivum; bacteria; fungi; gene expression; genes; gibberellic acid; glycosides; hybrids; methyl jasmonate; parents; wheat; xylanases
- Abstract:
- ... Xylanase inhibitor TAXI-I gene was cloned from wheat (Triticum aestivum L.) and then TAXI-I encoding sequence was expressed in Escherichia coli. The recombinant TAXI-I protein inhibited glycoside hydrolase (GH) family 11 xylanases in Aspergillus niger (Anx; a fungal xylanase), and Thermomonospora fusca (Tfx; a bacterial xylanase), and also inhibited hybrid xylanases Atx (a hybrid xylanase whose pa ...
- DOI:
- 10.1007/s10535-010-0025-z
- https://doi.org/10.1007/s10535-010-0025-z
- Author:
- Díaz-Vivancos, P.; Barba-Espín, G.; Clemente-Moreno, M. J.; Hernández, J. A.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 76-82
- ISSN:
- 0006-3134
- Subject:
- antioxidants; ascorbate oxidase; ascorbate peroxidase; catalase; enzyme activity; glutathione; glutathione dehydrogenase (ascorbate); monodehydroascorbate reductase (NADH); peas; peroxidase; roots; seedlings; shoots; superoxide dismutase; tissues
- Abstract:
- ... The antioxidative system was studied during the development of pea plants. The reduced glutathione (GSH) content was higher in shoots than in roots, but a greater redox state of glutathione existed in roots compared with shoots, at least after 7 d of growth. The 3-d-old seedlings showed the highest content of oxidised ascorbate (DHA), which correlated with the ascorbate oxidase (AAO) activity. Als ...
- DOI:
- 10.1007/s10535-010-0011-5
- https://doi.org/10.1007/s10535-010-0011-5
- Author:
- Osório, M. L.; Osório, J.; Romano, A.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 415-422
- ISSN:
- 0006-3134
- Subject:
- Rhododendron ponticum; acclimation; chlorophyll; electron transfer; energy; fluorescence; light intensity; micropropagation; photoinhibition
- Abstract:
- ... The aim of this study was to investigate acclimation of micropropagated plants of Rhododendron ponticum subsp. baeticum to different irradiances and recovery after exposure to high irradiance. Plants grown under high (HL) or intermediate (IL) irradiances displayed higher values of maximum electron transport rate (ETRmax) and light saturation coefficient (Ek) than plants grown under low irradiance ...
- DOI:
- 10.1007/s10535-010-0076-1
- https://doi.org/10.1007/s10535-010-0076-1
- Author:
- Lu, X.; Zhou, W.; Gao, F.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 798-800
- ISSN:
- 0006-3134
- Subject:
- Citrus sinensis; anthocyanins; biosynthesis; chromosomes; fluorescence in situ hybridization; genes; karyotyping; metaphase; oranges; ribosomal DNA
- Abstract:
- ... Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S r ...
- DOI:
- 10.1007/s10535-010-0146-4
- https://doi.org/10.1007/s10535-010-0146-4
- Author:
- You, J. F.; Hou, N. N.; Xu, M. Y.; Zhang, H. M.; Yang, Z. M.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 766-768
- ISSN:
- 0006-3134
- Subject:
- Glycine max; aluminum; citrates; cultivars; cycloheximide; exudation; mitochondrial membrane; plasma membrane; protein synthesis; protein synthesis inhibitors; pyridoxal; roots; secretion; soybeans; transporters
- Abstract:
- ... To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor, anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated after 4 h of Al ...
- DOI:
- 10.1007/s10535-010-0138-4
- https://doi.org/10.1007/s10535-010-0138-4
- Author:
- Gangopadhyay, M.; Chakraborty, D.; Dewanjee, S.; Bhattacharya, S.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 793-797
- ISSN:
- 0006-3134
- Subject:
- DNA; Zephyranthes; bulbs; carbendazim; explants; genomics; gibberellic acid; indole butyric acid; mercuric chloride; microbial contamination; micropropagation; microsatellite repeats; ornamental plants; polymerase chain reaction; roots; shoots; sucrose
- Abstract:
- ... Zephyr lily (Zephyranthes grandiflora), an important ornamental plant has been micropropagated in vitro after controlling microbial contamination by a pretreatment with 0.2 % Bavistin and 0.1 % Pantomycin for 4 h before final sterilization with 0.1 % mercuric chloride. In 67 % of the sterile cultures, 11 shoots on average were regenerated directly from basal half of bulb scales in Murashige and Sk ...
- DOI:
- 10.1007/s10535-010-0145-5
- https://doi.org/10.1007/s10535-010-0145-5
- Author:
- Mani, T.; Manjula, S.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 377-380
- ISSN:
- 0006-3134
- Subject:
- Piper; antifungal properties; black pepper; breeding; cDNA libraries; complementary DNA; genes; point mutation
- Abstract:
- ... In the present study, we report the cloning and sequence characterization of two isoforms of osmotin, an antifungal PR-5 gene homologue, from a salicylic acid-induced subtracted cDNA library earlier generated in Piper colubrinum. The larger form of the gene is 693 bp long, encoding a 21.5 kDa protein. The smaller form comprises a 543 bp long coding sequence which code for a protein of 16.4 kDa. A ...
- DOI:
- 10.1007/s10535-010-0068-1
- https://doi.org/10.1007/s10535-010-0068-1
- Author:
- Walker, D. J.; Romero, P.; Correal, E.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 293-298
- ISSN:
- 0006-3134
- Subject:
- Bituminaria bituminosa; cold; cold tolerance; electrolytes; freezing; leaves; osmotic pressure; osmotolerance; proline; shrubs; spring; sugars; temperature; winter
- Abstract:
- ... We determined the cold (freezing) tolerance of five Spanish populations of the perennial shrub Bituminaria bituminosa (L.) C.H. Stirton (Fabaceae), as the temperature at which 50 % of leaf electrolytes are released (LT₅₀) using leaves of field-grown plants, obtained in two winters and one spring. The freezing tolerance was greater in winter and reflected the minimum temperatures at the original si ...
- DOI:
- 10.1007/s10535-010-0051-x
- https://doi.org/10.1007/s10535-010-0051-x
- Author:
- Beharav, A.; Maras, M.; Kitner, M.; Šuštar-Vozlič, J.; Sun, G. L.; Doležalová, I.; Lebeda, A.; Meglič, V.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 54-60
- ISSN:
- 0006-3134
- Subject:
- DNA; Lactuca saligna; Phaseolus vulgaris; Triticum turgidum subsp. dicoccoides; amplified fragment length polymorphism; beans; genetic variation; lettuce; population structure; random amplified polymorphic DNA technique; self-pollination
- Abstract:
- ... Three genetic similarity coefficients were estimated and compared for their usefulness: simple matching (S SM), Jaccard's (S J) and Dice's (S D), all based on dominant markers data from individuals representing predominantly self-pollinating species. AFLP markers were used to analyze 139 Phaseolus vulgaris L. (common bean) and 67 Lactuca saligna L. (least lettuce) accessions, and RAPD markers were ...
- DOI:
- 10.1007/s10535-010-0008-0
- https://doi.org/10.1007/s10535-010-0008-0
- Author:
- Alizadeh, H.; Leung, D. W. M.; Cole, A. L. J.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 195-197
- ISSN:
- 0006-3134
- Subject:
- phytohemagglutinin; kidney beans; cotyledons; Phaseolus vulgaris; alpha-amylase
- Abstract:
- ... It is hypothesized that since protein α-amylase inhibitor (α-AI) and stimulator might be present together in red kidney bean (Phaseolus vulgaris L.) seeds, their in vitro interactions might influence their detection and quantification. Assay of α-AI using extracts from the embryonic axes revealed an unexpected finding in that the extracts stimulated rather than inhibited α-amylase activity. The co ...
- DOI:
- 10.1007/s10535-010-0034-y
- https://doi.org/10.1007/s10535-010-0034-y
- Author:
- Filek, M.; Biesaga-Kościelniak, J.; Marcińska, I.; Cvikrová, M.; Macháčková, I.; Krekule, J.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 483-487
- ISSN:
- 0006-3134
- Subject:
- cold treatment; germination; leaves; putrescine; roots; seedlings; spermidine; vernalization; winter wheat; zearalenone
- Abstract:
- ... The contents of endogenous free and conjugated polyamines, putrescine (Put) and spermidine (Spd), were determined during 9 week of vernalization (at 5 °C) in winter wheat seedlings cultivated on Murashige and Skoog media without (MS0) and with 2 mg dm⁻³ zearalenone (MSZEN). At the 4th week of chilling treatment, which is sufficient to induce generative development in 30 % of plants, the marked inc ...
- DOI:
- 10.1007/s10535-010-0085-0
- https://doi.org/10.1007/s10535-010-0085-0
- Author:
- Mirzaghaderi, G.; Karimzadeh, G.; Hassani, H. S.; Jalali-Javaran, M.; Baghizadeh, A.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 252-258
- ISSN:
- 0006-3134
- Subject:
- chromosome banding; chromosome number; chromosomes; crossing; cultivars; cytogenetic analysis; genotype; hybrids; in situ hybridization; meiosis; salt tolerance; seeds; wheat
- Abstract:
- ... The new salt tolerant cereal, Tritipyrum (2n=6x=42, AABBEbEb) offers potential to introduce desirable characters for wheat improvements. This study was aimed to generate a segregating population from Iranian local wheat cultivars (2n=6x=42, AABBDD) and Tritipyrum crosses, study of the meiotic behaviour in F₂ hybrids and identification of Eb chromosomes in F₃ individuals. Results showed meiotic abn ...
- DOI:
- 10.1007/s10535-010-0044-9
- https://doi.org/10.1007/s10535-010-0044-9
- Author:
- Diao, W. -P.; Bao, S. -Y.; Jiang, B.; Cui, L.; Qian, C. -T.; Chen, J. -F.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 373-376
- ISSN:
- 0006-3134
- Subject:
- Cucumis sativus; anaphase; chromosome segregation; chromosomes; cucumbers; cytokinesis; gametophytes; germination; meiosis; metaphase; pollen; telophase
- Abstract:
- ... With improved staining and chromosome preparation techniques, meiosis of pollen mother cells (PMCs) and male gametophyte development in autotetraploid cucumber (Cucumis sativus L.) was studied to understand the correlation between chromosomes behaviour and fertility. Various chromosome configurations, e.g. multivalent, quadrivalents, trivalents, bivalents and univalents were observed in most PMCs ...
- DOI:
- 10.1007/s10535-010-0067-2
- https://doi.org/10.1007/s10535-010-0067-2
31. Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis
- Author:
- Kumar, P.; Singhal, V. K.; Kaur, D.; Kaur, S.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 181-184
- ISSN:
- 0006-3134
- Subject:
- Clematis; chromatin; chromosome number; meiosis; pollen; prophase; stickiness; tetraploidy; India
- Abstract:
- ... Present cytological investigations from the cold desert regions of Lahaul-Spiti and Kinnaur (India) record the first ever tetraploid (2n=32) chromosome count and cytomixis in Clematis orientalis L. var. acutifolia Hook. f. et Thoms. The phenomenon of cytomixis (9.33-29.80 %) involving chromatin transfer among 2-3 proximate pollen mother cells (PMCs) during male meiosis occurs through narrow and br ...
- DOI:
- 10.1007/s10535-010-0031-1
- https://doi.org/10.1007/s10535-010-0031-1
- Author:
- Shah, J. M.; Veluthambi, K.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 443-450
- ISSN:
- 0006-3134
- Subject:
- Agrobacterium radiobacter; Dianthus caryophyllus; Mungbean yellow mosaic virus; Oryza sativa; blight; callus; gene expression; genes; leaves; promoter regions; rice; tobacco; toxicity; transgenic plants
- Abstract:
- ... The DIANTHIN gene encoding a ribosome-inactivating protein (RIP) from Dianthus caryophyllus L. was tested for negative selection in tobacco and rice. Tobacco leaf discs and scutellum-derived callus of rice were transformed with Agrobacterium tumefaciens strain LBA4404 (pSB1, pJAS1). pJAS1 harbors the DIANTHIN gene under the control of the CaMV 35S promoter. Tobacco transformation efficiency, in co ...
- DOI:
- 10.1007/s10535-010-0080-5
- https://doi.org/10.1007/s10535-010-0080-5
33. Development of AFLP and STS markers linked to a waterlogging tolerance in Korean soybean landraces
- Author:
- Kang, S. -Y.; Lee, K. J.; Lee, G. -J.; Kim, J. -B.; Chung, S. -J.; Song, J. Y.; Lee, B. -M.; Kim, D. S.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 61-68
- ISSN:
- 0006-3134
- Subject:
- DNA primers; Glycine max; amplified fragment length polymorphism; chlorophyll; cluster analysis; flooded conditions; gamma radiation; genotype; glucose; landraces; malondialdehyde; marker-assisted selection; mutants; mutation; soybeans
- Abstract:
- ... Among the 400 soybean (Glycine max) landraces, we selected 3 tolerant (KAS150-9, KAS160-15, and KAS170-9) and 3 susceptible lines (KAS160-14, KAS160-20, and KAS201-6-1) by the survival percentage and injury scores. Susceptible lines showed decrease in chlorophyll content and increase in glucose and malondialdehyde (MDA) contents under waterlogging stress, while tolerant lines did not change signif ...
- DOI:
- 10.1007/s10535-010-0009-z
- https://doi.org/10.1007/s10535-010-0009-z
- Author:
- Ahmad, N.; Anis, M.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 748-752
- ISSN:
- 0006-3134
- Subject:
- Vitex negundo; acetic acid; artificial seeds; calcium chloride; concentration of production; culture media; encapsulation; germination; kinetin; naphthaleneacetic acid; plantlets; sodium alginate
- Abstract:
- ... An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na₂-alginate with 100 mM CaCl₂ has been found to be optimum concentration for the production of uniform synthetic ...
- DOI:
- 10.1007/s10535-010-0134-8
- https://doi.org/10.1007/s10535-010-0134-8
- Author:
- Khurana-Kaul, V.; Kachhwaha, S.; Kothari, S. L.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 369-372
- ISSN:
- 0006-3134
- Subject:
- Jatropha curcas; benzyladenine; buds; callus; callus formation; copper sulfate; explants; gibberellic acid; indole butyric acid; leaves; plantlets; shoots; thidiazuron
- Abstract:
- ... Leaf explants of Jatropha curcas cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ; 0.90 μM) in combination with indole-3-butyric acid (IBA; 0.98μM) produced adventitious shoot buds directly on the surface of the explants without formation of intervening callus while shoot bud formation was accompanied with callus formation on medium supplemented with 6-benzylaminopu ...
- DOI:
- 10.1007/s10535-010-0066-3
- https://doi.org/10.1007/s10535-010-0066-3
- Author:
- Kiran Ghanti, S.; Sujata, K. G.; Srinath Rao, M.; Kavi Kishor, P. B.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 121-125
- ISSN:
- 0006-3134
- Subject:
- 2,4,5-T; 2,4-D; Cicer arietinum; abscisic acid; chickpeas; cotyledons; cultivars; developmental stages; explants; kinetin; naphthaleneacetic acid; picloram; plantlets; somatic embryogenesis; somatic embryos
- Abstract:
- ... A protocol for plant regeneration via somatic embryogenesis was developed in two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri. Somatic embryos were induced from immature cotyledons on Murashige and Skoog's (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-naphthaleneacetic acid (NAA) and ...
- DOI:
- 10.1007/s10535-010-0018-y
- https://doi.org/10.1007/s10535-010-0018-y
- Author:
- Ma, G. H.; He, C. X.; Ren, H.; Zhang, Q. M.; Li, S. J.; Zhang, X. H.; Eric, B.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 361-365
- ISSN:
- 0006-3134
- Subject:
- acclimation; activated carbon; adventitious roots; adventitious shoots; benzyladenine; cytokinins; endangered species; explants; indole butyric acid; kinetin; leaves; organogenesis; pH; plantlets; rooting; somatic embryogenesis; somatic embryos; thidiazuron
- Abstract:
- ... An efficient propagation system via somatic embryogenesis and shoot organogenesis and plant regeneration system for endangered species Primulina tabacum Hance was established. Thidiazuron (TDZ) was the key plant growth regulator for inducing somatic embryogenesis and kinetin (KIN) and 6-benzylaminopurine (BAP) were the key cytokinins for inducing shoot organogenesis from leaf explants. TDZ combine ...
- DOI:
- 10.1007/s10535-010-0064-5
- https://doi.org/10.1007/s10535-010-0064-5
- Author:
- Ercan, S.; Ertugrul, F.; Aydin, Y.; Akfirat, F. S.; Hasancebi, S.; Akan, K.; Mert, Z.; Bolat, N.; Yorgancilar, O.; Altinkut-Uncuoglu, A.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 691-696
- ISSN:
- 0006-3134
- Subject:
- DNA; Triticum aestivum; expressed sequence tags; genetic markers; genotype; hybrids; microsatellite repeats; seedlings; stripe rust; wheat
- Abstract:
- ... Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F₂ seedlings were constructed. 78 EST-SSR prime ...
- DOI:
- 10.1007/s10535-010-0122-z
- https://doi.org/10.1007/s10535-010-0122-z
- Author:
- Li, W. M.; Tao, Y.; Yao, Y. X.; Hao, Y. J.; You, C. X.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 639-646
- ISSN:
- 0006-3134
- Subject:
- Arabidopsis; Malus domestica; amino acid sequences; amino acids; apples; complementary DNA; flowering; gene overexpression; genes; leaves; loci; open reading frames; reverse transcriptase polymerase chain reaction
- Abstract:
- ... To get insight into mechanism by which apple tree (Malus domestica Borkh.) regulates flowering, two apple flowering locus T (FT) homologues, MdFT1 and MdFT2, were isolated from the leaf cDNAs of cultivar Gala. The open reading frames (ORFs) of two MdFTs encoded 174 amino acids. The deduced amino acid sequence of MdFT1 and MdFT2 showed 94.3 % similarity to each other, while 72.6 and 76.0 % to AtFT ...
- DOI:
- 10.1007/s10535-010-0114-z
- https://doi.org/10.1007/s10535-010-0114-z
- Author:
- Mýtinová, Z.; Motyka, V.; Haisel, D.; Gaudinová, A.; Lubovská, Z.; Wilhelmová, N.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 461-470
- ISSN:
- 0006-3134
- Subject:
- Arabidopsis thaliana; Nicotiana tabacum; abscisic acid; antioxidants; ascorbate peroxidase; catalase; cytokinins; enzyme activity; genes; glutathione-disulfide reductase; growth retardation; indole acetic acid; leaves; phenotype; promoter regions; roots; salinity; superoxide dismutase; tobacco; transgenic plants; water stress; zinc
- Abstract:
- ... The responses of antioxidant enzymes (AOE) ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in soluble protein extracts from leaves and roots of tobacco (Nicotiana tabacum L. cv. Samsun NN) plants to the drought stress, salinity and enhanced zinc concentration were investigated. The studied tobacco included wild-type (WT) and transgenic plants ...
- DOI:
- 10.1007/s10535-010-0082-3
- https://doi.org/10.1007/s10535-010-0082-3
- Author:
- Ding, W.; Song, L.; Wang, X.; Bi, Y.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 607-613
- ISSN:
- 0006-3134
- Subject:
- Phragmites australis; abscisic acid; ascorbate peroxidase; callus; catalase; cell viability; ecotypes; fluridone; growth retardation; heat stress; heat tolerance; hydrogen peroxide; malondialdehyde; peroxidase; stress tolerance; superoxide dismutase; swamps
- Abstract:
- ... Dune reed (DR) and swamp reed (SR) are two ecotypes of reed (Phragmites communis Trin.) that displayed differences in stress tolerance. To uncover the molecular basis for such difference, the effects of heat stress were studied using the calli derived from the two ecotypes. Heat stress caused increased ion leakage, inhibited growth, decreased cell viability, and elevated hydrogen peroxide (H₂O₂) a ...
- DOI:
- 10.1007/s10535-010-0110-3
- https://doi.org/10.1007/s10535-010-0110-3
- Author:
- Saharan, V.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 740-742
- ISSN:
- 0006-3134
- Subject:
- Asparagus officinalis; culture media; gibberellic acid; saponins; shoots; tissue culture
- Abstract:
- ... Effect of gibberellic acid (GA₃) combined with saponin on shoot elongation of Asparagus officinalis was evaluated in tissue culture. Addition of saponin to GA₃ supplemented Murashige and Skoog (MS) basal medium showed a dose depended effect on shoot length of Asparagus officinalis. However, increasing concentration of saponin above 3.0 mg dm⁻³ decreased shoot length and showed yellowing and thinni ...
- DOI:
- 10.1007/s10535-010-0132-x
- https://doi.org/10.1007/s10535-010-0132-x
43. Effect of photoperiod during growth of Chenopodium rubrum mother plants on properties of offspring
- Author:
- Mitrović, A.; Bogdanović, J.; Giba, Z.; Ćulafić, L.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 735-739
- ISSN:
- 0006-3134
- Subject:
- Chenopodium rubrum; flowering; in vitro culture; mothers; photoperiod; progeny; seed germination; seed maturation
- Abstract:
- ... Using in vitro culture, we determined the effect of photoperiod during growth of Chenopodium rubrum mother plants on vegetative and reproductive development of offspring. Photoperiod during flowering induction of mother plants (the first 6 d after the germination) has the key influence on seed germination and offspring growth, while offspring flowering and seed maturation is determined by photoper ...
- DOI:
- 10.1007/s10535-010-0131-y
- https://doi.org/10.1007/s10535-010-0131-y
- Author:
- Moussa, H. R.; El-Gamal, S. M.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 315-320
- ISSN:
- 0006-3134
- Subject:
- Triticum aestivum; abscisic acid; biomass; cadmium; chlorophyll; chloroplasts; hydrogen peroxide; malondialdehyde; phosphoenolpyruvate carboxylase; proline; root growth; roots; salicylic acid; seeds; toxicity; ultrastructure; water content; wheat
- Abstract:
- ... Cadmium (100, 400 and 1000 μM CdCl₂) treatments resulted in the inhibition of root dry biomass, root elongation and increased Cd accumulation in wheat (Triticum aestivum L.) roots. Further, these treatments decreased relative water content, chlorophyll content, ¹⁴CO₂-fixation, activities of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase and abscisic acid content while in ...
- DOI:
- 10.1007/s10535-010-0054-7
- https://doi.org/10.1007/s10535-010-0054-7
- Author:
- Ionenko, I. F.; Anisimov, A. V.; Dautova, N. R.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 488-494
- ISSN:
- 0006-3134
- Subject:
- activation energy; aquaporins; corn; lipid bilayers; magnetic fields; mercuric chloride; nuclear magnetic resonance spectroscopy; surface area; water temperature
- Abstract:
- ... The mean effective water self-diffusion coefficient in maize root segments under the effect of aquaporin blocker (mercuric chloride, 0.1 mM) was measured using the spin-echo NMR method with pulsed magnetic field gradient within the temperature range from 10 to 35 °C. HgCl₂ caused the reduction in water diffusion by 30 % as compared to the control samples. Temperature dependences of water self-diff ...
- DOI:
- 10.1007/s10535-010-0086-z
- https://doi.org/10.1007/s10535-010-0086-z
46. Effect of zinc and cadmium on physiological and production characteristics in Matricaria recutita
- Author:
- Kummerová, M.; Zezulka, Š.; Kráľová, K.; Masarovičová, E.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 308-314
- ISSN:
- 0006-3134
- Subject:
- Matricaria chamomilla; cadmium; carotenoids; chamomile; chlorophyll; fluorescence; hydroponics; pigments; respiratory rate; roots; shoots; zinc
- Abstract:
- ... Effects of zinc (12-180 μM) alone and in mixtures with 12 μM Cd on metal accumulation, dry masses of roots and shoots, root respiration rate, variable to maximum fluorescence ratio (FV/FM), and content of photosynthetic pigments were studied in hydroponically cultivated chamomile (Matricaria recutita) plants. The content of Zn in roots and shoots increased with the increasing external Zn concentra ...
- DOI:
- 10.1007/s10535-010-0053-8
- https://doi.org/10.1007/s10535-010-0053-8
- Author:
- Chai, Y. Y.; Jiang, C. D.; Shi, L.; Shi, T. S.; Gu, W. B.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 145-148
- ISSN:
- 0006-3134
- Subject:
- Sorghum bicolor; catalase; germination; glutathione-disulfide reductase; growth retardation; malondialdehyde; peroxidase; proline; protein content; roots; salinity; seedlings; shoots; sodium chloride; spermine; sweet sorghum
- Abstract:
- ... Seedlings of Sorghum bicolor (L.) Moench were subjected to 180 mM NaCl with or without 0.25 mM spermine (SPM) for 7 d. NaCl treatment resulted in the inhibition of growth and increased the content of free proline, soluble protein and malondialdehyde (MDA). Additionally, it also enhanced the activity of catalase (CAT), peroxidase (POX) in both shoots and roots, while decreased that of glutathione r ...
- DOI:
- 10.1007/s10535-010-0023-1
- https://doi.org/10.1007/s10535-010-0023-1
- Author:
- Šmídková, M.; Holá, M.; Angelis, K. J.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 777-780
- ISSN:
- 0006-3134
- Subject:
- Physcomitrella patens; biolistics; gold; homologous recombination; mosses and liverworts; plasmids; transgenes
- Abstract:
- ... High rates of homologous recombination (HR) in comparison to other plants make the moss Physcomitrella patens an attractive model organism for genetic studies as well as biotechnological applications. We describe a simple protocol for the efficient biolistic transformation of protonemal tissue with minimum tissue handling steps. The transformation efficiency depends on the biolistic conditions. Th ...
- DOI:
- 10.1007/s10535-010-0141-9
- https://doi.org/10.1007/s10535-010-0141-9
- Author:
- Purkayastha, J.; Sugla, T.; Paul, A.; Solleti, S. K.; Mazumdar, P.; Basu, A.; Mohommad, A.; Ahmed, Z.; Sahoo, L.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 13-20
- ISSN:
- 0006-3134
- Subject:
- Cauliflower mosaic virus; Jatropha curcas; Southern blotting; beta-glucuronidase; culture media; explants; gene transfer; genetic transformation; gibberellic acid; gold; kanamycin; osmotic treatment; particle size; plant establishment; plantlets; plasmids; polymerase chain reaction; shoots; transgenes; transgenic plants
- Abstract:
- ... An efficient and reproducible in vitro plant regeneration system from shoot apices was developed in Jatropha curcas. Benzylaminopurine (BAP; 2.5 μM) was most effective in inducing an average of 6.2 shoots per shoot apex. Incorporation of gibberellic acid (GA3; 0.5 μM) to basal medium was found essential for elongation of shoots. The BAP-habituated mother explants continuously produced shoots durin ...
- DOI:
- 10.1007/s10535-010-0003-5
- https://doi.org/10.1007/s10535-010-0003-5
- Author:
- Kaur, P.; Bansal, K. C.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 344-348
- ISSN:
- 0006-3134
- Subject:
- Agrobacterium radiobacter; Solanum lycopersicum var. lycopersicum; Southern blotting; flowers; fruits; gene expression; indole acetic acid; inorganic salts; kanamycin; leaves; neomycin; reverse transcriptase polymerase chain reaction; shoots; tomatoes; transgenes; transgenic plants; vitamins; zeatin
- Abstract:
- ... Cotyledonary leaves of 9-d-old tomato (Lycopersicon esculentum Mill.) were co-cultivated with Agrobacterium tumefaciens GV 3101 harboring binary vector pBI101 containing kanamycin resistance gene (npt II) as selection marker. Murashige and Skoog (MS) inorganic salts with Gamborg's B5 vitamins supplemented with optimized concentrations of zeatin riboside and indole-acetic acid resulted in enhanced ...
- DOI:
- 10.1007/s10535-010-0060-9
- https://doi.org/10.1007/s10535-010-0060-9
- Author:
- Huang, Z. -C.; Zeng, F. -H.; Lu, X. -Y.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 131-134
- ISSN:
- 0006-3134
- Subject:
- Eucalyptus urophylla; benzyladenine; buds; gibberellic acid; greenhouses; hypocotyls; indole acetic acid; indole butyric acid; naphthaleneacetic acid; plantlets; rooting; seedlings; shoots; urea
- Abstract:
- ... Seedling hypocotyls were used as explants to establish a regeneration protocol for Eucalyptus urophylla and N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl] urea (PBU), one kind of di-substituted urea, was found useful growth regulator. The hypocotyls incubated on a modified Murashige and Skoog medium (SPCa), supplemented with 6.6 μM PBU and 0.57 μM indole-3-acetic acid (IAA) dedifferentiated and form ca ...
- DOI:
- 10.1007/s10535-010-0020-4
- https://doi.org/10.1007/s10535-010-0020-4
- Author:
- Janeczko, A.; Swaczynová, J.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 477-482
- ISSN:
- 0006-3134
- Subject:
- brassinolide; high performance liquid chromatography; leaves; mass spectrometry; plant age; seed treatment; seedlings; seeds; soaking; spraying; wheat
- Abstract:
- ... The aim of the study was to examine the effect of exogenous 24-epibrassinolide on its uptake and content of endogenous brassinosteroids in wheat seedlings. 24-Epibrassinolide was applied at two concentrations (0.1 and 2.0 μM) and in three different methods: by soaking seeds, by drenching and by spraying plants. Brassinosteroids were determined by high-performance liquid chromatography combined wit ...
- DOI:
- 10.1007/s10535-010-0084-1
- https://doi.org/10.1007/s10535-010-0084-1
53. Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor
- Author:
- Aragón, C.; Carvalho, L.; González, J.; Escalona, M.; Amâncio, S.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 237-244
- ISSN:
- 0006-3134
- Subject:
- Musa; acclimation; ascorbate peroxidase; bioreactors; catalase; genes; glutathione dehydrogenase (ascorbate); glutathione transferase; glutathione-disulfide reductase; leaves; micropropagation; monodehydroascorbate reductase (NADH); nitroblue tetrazolium; plantlets; polymerization; superoxide dismutase; transgenic plants
- Abstract:
- ... Plantain (Musa ABB CEMSA 3/4) plantlets were micropropagated in temporary immersion bioreactors (TIB) or in gelled medium (GM). After ex vitro transfer ROS accumulation was determined by infiltrating leaves with nitroblue tetrazolium (NBT) and 3,3′-diaminobenzidine (DAB). Stomatal cells were more stained with NBT and DAB in GM plants than in TIB plants, but the difference disappeared at the end of ...
- DOI:
- 10.1007/s10535-010-0042-y
- https://doi.org/10.1007/s10535-010-0042-y
54. Exogenously-supplied trehalose protects thylakoid membranes of winter wheat from heat-induced damage
- Author:
- Luo, Y.; Li, F.; Wang, G. P.; Yang, X. H.; Wang, W.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 495-501
- ISSN:
- 0006-3134
- Subject:
- winter wheat; proteins; electrolytes; malondialdehyde; thylakoids; hydrogen peroxide; seedlings; linoleate 13S-lipoxygenase; superoxide anion; heat stress; trehalose; photosynthesis
- Abstract:
- ... The effects of trehalose pretreatment on thylakoid membranes of winter wheat were investigated under heat stress. Under normal growth conditions, the winter wheat synthesized 502 μg g⁻¹(f.m.) trehalose, which increased to 1250 μg g⁻¹(f.m.) under heat stress and to 1658 μg g⁻¹(f.m.) in trehalose-pretreated seedlings. Under heat stress, proteins in the thylakoid membranes and the photosynthetic capa ...
- DOI:
- 10.1007/s10535-010-0087-y
- https://doi.org/10.1007/s10535-010-0087-y
- Author:
- Shekhawat, U. K. S.; Ganapathi, T. R.; Srinivas, L.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 720-724
- ISSN:
- 0006-3134
- Subject:
- Agrobacterium radiobacter; Santalum album; Western blotting; additives; culture media; enzyme-linked immunosorbent assay; genetically modified organisms; growing media; hepatitis B; hepatitis B antigens; kanamycin; reverse transcriptase polymerase chain reaction; surface antigens; trehalose
- Abstract:
- ... Embryogenic cell suspension cultures of Santalum album were transformed with Agrobacterium tumefaciens harboring pD35SHER plant expression vector having hepatitis B small surface antigen (HBsAg) with a C-terminal ER retention signal. The transformed colonies were selected on culture medium supplemented with kanamycin and subsequently the transgenic nature of these colonies was confirmed by PCR ana ...
- DOI:
- 10.1007/s10535-010-0128-6
- https://doi.org/10.1007/s10535-010-0128-6
- Author:
- Bruňáková, K.; Košuth, J.; Katkovčinová, Z.; Lázárová, M.; Čellárová, E.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 515-519
- ISSN:
- 0006-3134
- Subject:
- Taxus baccata; biosynthesis; genes; germination; high performance liquid chromatography; messenger RNA; metabolites; paclitaxel; reverse transcriptase polymerase chain reaction; seedlings; shoots; stems; taxanes
- Abstract:
- ... The spatial and temporal expression of dbat and dbtnbt genes involved in the later steps of paclitaxel biosynthesis in relation to baccatin III and paclitaxel accumulation in Taxus baccata L. germinating embryos and seedlings was investigated. The steady-state of mRNA transcripts was measured by quantitative real time polymerase chain reaction (qRT-PCR), the content of taxanes was determined by HP ...
- DOI:
- 10.1007/s10535-010-0090-3
- https://doi.org/10.1007/s10535-010-0090-3
57. Expressions and dimerization affinities of three highly identical APETALA3 genes in Brassica napus
- Author:
- Zhou, L.; Zhou, Y. -T.; Wang, M. -L.; Wang, H. -Y.; Zhao, Y.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 33-40
- ISSN:
- 0006-3134
- Subject:
- Brassica napus; amino acid sequences; clones; complementary DNA; corolla; dimerization; gene expression; genes; open reading frames; proteins; reverse transcriptase polymerase chain reaction; sequence analysis; stamens; transcription (genetics); two hybrid system techniques
- Abstract:
- ... Three highly identical cDNA clones of APETALA3 (AP3) gene, BnAP3-2, BnAP3-3 and BnAP3-4 were isolated from Brassica napus L. by RT-PCR. The sequence analysis showed that all the three AP3 cDNAs contained a complete open reading frame. Their nucleotide sequences had 91-97 % similarity and their predicted amino acid sequences shared 93-98 % identity. Real-time quantitative RT-PCR result showed that ...
- DOI:
- 10.1007/s10535-010-0005-3
- https://doi.org/10.1007/s10535-010-0005-3
- Author:
- Kumar, A.; Aggarwal, D.; Gupta, P.; Reddy, M. S.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 601-606
- ISSN:
- 0006-3134
- Subject:
- Chlorophytum borivilianum; DNA; agar; benzyladenine; culture media; cytokinins; gelation; gelling agents; heat; humidity; indole butyric acid; kinetin; light intensity; microsatellite repeats; random amplified polymorphic DNA technique; rooting; shoots; soil; sucrose; temperature; vermicomposts
- Abstract:
- ... The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116-290 mM in the basal medium (BM) promoted shoot multipl ...
- DOI:
- 10.1007/s10535-010-0109-9
- https://doi.org/10.1007/s10535-010-0109-9
- Author:
- Singh-Rawal, P.; Jajoo, A.; Bharti, S.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 556-560
- ISSN:
- 0006-3134
- Subject:
- absorption; ambient temperature; electron transfer; electron transport chain; energy; fluorescence; photosystem I; spinach; thylakoids
- Abstract:
- ... The effects of fluoride on the photosynthetic electron transport chain have been studied in spinach thylakoid membranes. Inhibition in photosystem (PS) 2 electron transport rates and a subsequent increase in PS 1 electron transport rate indicated a possibility of state transitions being a mechanism of fluoride action. This hypothesis was further confirmed by the increase in fluorescence emission F ...
- DOI:
- 10.1007/s10535-010-0099-7
- https://doi.org/10.1007/s10535-010-0099-7
- Author:
- Zhao, L. -L.; Song, L. -Q.; You, C. -X.; Moriguchi, T.; Hao, Y. -J.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 631-638
- ISSN:
- 0006-3134
- Subject:
- adenosylmethionine decarboxylase; apples; catalase; cold stress; drought; gene expression regulation; gene overexpression; lipid peroxidation; malondialdehyde; polyamines; polyethylene glycol; promoter regions; reporter genes; sodium chloride; superoxide dismutase; temperature; tobacco; transcription (genetics); transgenic plants
- Abstract:
- ... The expression of MdSAMDC2 gene, which encodes S-adenosylmethionine decarboxylase (SAMDC) in apple, was up-regulated by low temperature, salt and drought stresses. To identify its in vivo biological functions in the responses to stresses, the promoter region of MdSAMDC2 was isolated and characterized by analyzing the cis-acting regulatory elements and GUS reporter gene by an Agrobacterium-mediated ...
- DOI:
- 10.1007/s10535-010-0113-0
- https://doi.org/10.1007/s10535-010-0113-0
- Author:
- Molnár, I.; Molnár-Láng, M.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 259-264
- ISSN:
- 0006-3134
- Subject:
- Aegilops biuncialis; Triticum aestivum; chromatin; chromosome pairing; genotype; hybrids; in situ hybridization; introgression; meiosis; metaphase; mutants; wheat
- Abstract:
- ... The Triticum aestivum — Aegilops biuncialis (2n=4x=28; UbUbMbMb) disomic addition lines 2Mb, 3Mb, 7Mb and 3Ub were crossed with the wheat cv. Chinese Spring ph1b mutant genotype in order to induce homoeologous pairing, with the final goal of introgressing Ae. biuncialis chromatin into cultivated wheat. Wheat-Aegilops homoeologous chromosome pairing was studied in metaphase I of meiosis in the F₁ h ...
- DOI:
- 10.1007/s10535-010-0045-8
- https://doi.org/10.1007/s10535-010-0045-8
- Author:
- Carmona, S.; Alvarez, J. B.; Caballero, L.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 69-75
- ISSN:
- 0006-3134
- Subject:
- Triticum turgidum; alleles; durum wheat; genetic background; genetic variation; glutenins; loci; molecular weight; seed storage proteins
- Abstract:
- ... The objective of the current work was to analyse the variability of high and low molecular mass (HMM and LMM) glutenin subunits, along with some morphological characteristics in sixty Spanish accessions of rivet wheat (Triticum turgidum L. ssp. turgidum). The lines were grouped in sixteen botanical varieties and five additional types, according the morphological criteria. Up to 13 allelic variants ...
- DOI:
- 10.1007/s10535-010-0010-6
- https://doi.org/10.1007/s10535-010-0010-6
63. Genetic diversity in Passiflora species determined by morphological and molecular characteristics
- Author:
- Viana, A. J. C.; Souza, M. M.; Araújo, I. S.; Corrêa, R. X.; Ahnert, D.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 535-538
- ISSN:
- 0006-3134
- Subject:
- Passiflora; cluster analysis; flowers; fruits; genetic distance; genetic variation; intraspecific variation; leaf area; random amplified polymorphic DNA technique; seeds
- Abstract:
- ... Morphological and molecular characteristics were studied in six wild species of Passiflora. There were statistically significant differences among these six species for all characteristics studied. Intra-specific variability was observed for number of flowers, number of fruits, number of seeds, fruit length, fruit width and leaf area. Cluster analysis using morphological data showed three groups: ...
- DOI:
- 10.1007/s10535-010-0094-z
- https://doi.org/10.1007/s10535-010-0094-z
- Author:
- Sikdar, B.; Bhattacharya, M.; Mukherjee, A.; Banerjee, A.; Ghosh, E.; Ghosh, B.; Roy, S. C.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 135-140
- ISSN:
- 0006-3134
- Subject:
- Cucurbitaceae; DNA; cluster analysis; genetic markers; genetic variation; isozymes; microsatellite repeats; random amplified polymorphic DNA technique
- Abstract:
- ... Biochemical and molecular markers have been used on eleven species of Cucurbitaceae collected from lower Gangetic plains. Six enzyme systems were selected. Among 40 primers examined, 14 random amplified polymorphic DNA (RAPD) and 10 inter-simple sequence repeat (ISSR) primers were selected for the analysis. Generated RAPD (100) and ISSR (100) fragments showed high variations among the species. Jac ...
- DOI:
- 10.1007/s10535-010-0021-3
- https://doi.org/10.1007/s10535-010-0021-3
- Author:
- Sciacca, F.; Fichera, C.; Di Silvestro, S.; Conte, E.; Palumbo, M.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 198-200
- ISSN:
- 0006-3134
- Subject:
- DNA; DNA fingerprinting; Triticum turgidum; amplified fragment length polymorphism; cultivars; durum wheat; fluorescence; genetic relationships; genetic variation; genotype
- Abstract:
- ... The DNA of fifteen Italian cultivars of durum wheat (Triticum turgidum L. ssp. durum) were analyzed by in fluorescence amplified fragment length polymorphism (fAFLP) in order to obtain the characteristic fingerprintings of genotypes and assess their genetic relatedness. Among 64 combinations of fluorescence labelled primers, three different combinations were chosen as producing a total of 6630 AFL ...
- DOI:
- 10.1007/s10535-010-0035-x
- https://doi.org/10.1007/s10535-010-0035-x
- Author:
- Pei, Z.; Gao, J.; Chen, Q.; Wei, J.; Li, Z.; Luo, F.; Shi, L.; Ding, B.; Sun, S.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 653-658
- ISSN:
- 0006-3134
- Subject:
- Sorghum bicolor subsp. bicolor; alleles; chromosomes; cluster analysis; genetic variation; genotype; microsatellite repeats; sweet sorghum
- Abstract:
- ... To determine genetic diversity among 47 elite sweet sorghum (Sorghum bicolor ssp. bicolor L.) genotypes, 46 simple sequence repeat (SSR) markers evenly distributed on all 10 chromosomes were selected. All SSR markers used were polymorphic among the genotypes studied. A total of 228 alleles were identified with an average of 4.96 alleles per marker. Furthermore, the genotypes studied showed medium ...
- DOI:
- 10.1007/s10535-010-0116-x
- https://doi.org/10.1007/s10535-010-0116-x
- Author:
- Skuza, L.; Dyba, S. M.; Rogalska, S. M.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 169-172
- ISSN:
- 0006-3134
- Subject:
- Medicago sativa; alfalfa; genetic relationships; inbred lines; landraces; mitochondrial DNA; nucleotide sequences; seed set; soybeans; synthetic populations
- Abstract:
- ... Two synthetic populations (Syn-5 and Syn-7), four inbred lines and four landraces of alfalfa (Medicago sativa L.) were studied for genetic relatedness. These forms were characterized by different amount of seeds set and green mass yield. Two primer pairs of specific nucleotide sequences of mitochondrial DNA isolated from soybean were used. The mtDNA revealed the existence of significant polymorphi ...
- DOI:
- 10.1007/s10535-010-0028-9
- https://doi.org/10.1007/s10535-010-0028-9
- Author:
- Li, J. R.; Yu, K.; Wei, J. R.; Ma, Q.; Wang, B. Q.; Yu, D.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 395-399
- ISSN:
- 0006-3134
- Subject:
- proteins; perennials; gibberellic acid; linoleate 13S-lipoxygenase; chlorophyll; peroxidase; chlorophyllase; shoots
- Abstract:
- ... Chlorophyll (Chl) degradation was found to be related to the endogenous gibberellin (GA) content in shoots during senescence in the perennial plant Paris polyphylla var. yunnanensis (Franch.). Treatment with gibberellic acid (GA₃) significantly increased the content of endogenous GAs (GA₄ + GA₇), retarded the senescence of shoots, and the degradation of proteins and Chl. Chlorophyllase, Mg-dechela ...
- DOI:
- 10.1007/s10535-010-0072-5
- https://doi.org/10.1007/s10535-010-0072-5
- Author:
- Dmitrović, S.; Mitić, N.; Zdravković-Korać, S.; Vinterhalter, B.; Ninković, S.; Ćulafić, L. J.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 566-570
- ISSN:
- 0006-3134
- Subject:
- Chenopodium rubrum; Southern blotting; callus; explants; genes; genetically modified organisms; petioles; plant growth; polymerase chain reaction; roots; thidiazuron
- Abstract:
- ... Susceptibility of C. rubrum to Agrobacterium-mediated transformation was demonstrated by inoculating the petioles of in vitro grown plants with A. rhizogenes strain A4M70GUS. Hairy roots were produced in 8 % of explants. They were isolated and maintained on plant growth regulator-free solid or liquid half-strength Murashige and Skoog medium for two years. Hairy root fresh mass increased 30 — 90 fo ...
- DOI:
- 10.1007/s10535-010-0101-4
- https://doi.org/10.1007/s10535-010-0101-4
- Author:
- Xing, Y.; Yu, Y.; Luo, X.; Zhang, J. -N.; Zhao, B.; Guo, Y. -D.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 231-236
- ISSN:
- 0006-3134
- Subject:
- Solanum melongena; buds; cotyledons; eggplants; explants; flow cytometry; genetic markers; genetic stability; gibberellic acid; indole acetic acid; microsatellite repeats; organogenesis; random amplified polymorphic DNA technique; shoots; silver nitrate; somaclonal variation; sucrose; zeatin
- Abstract:
- ... A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm⁻³ zeatin, 0.1 mg dm⁻³ indoleacetic acid and 10 g dm⁻³ sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elong ...
- DOI:
- 10.1007/s10535-010-0041-z
- https://doi.org/10.1007/s10535-010-0041-z
- Author:
- Zhang, H.; Hu, L. -Y.; Li, P.; Hu, K. -D.; Jiang, C. -X.; Luo, J. -P.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 743-747
- ISSN:
- 0006-3134
- Subject:
- seed germination; peroxidase; dose response; seeds; catalase; ascorbate peroxidase; hydrogen peroxide; malondialdehyde; linoleate 13S-lipoxygenase; superoxide dismutase; wheat; Triticum aestivum; toxicity; chromium; hydrogen sulfide; esterases
- Abstract:
- ... Effects of H₂S on seed germination under chromium (Cr) stress were investigated in wheat (Triticum aestivum L.). Under Cr stress, the percentage of germination of wheat seeds decreased, but this decrease could be alleviated by pretreatment with NaHS, an H₂S donor, in a dose-dependent manner. Furthermore, NaHS significantly enhanced the activities of amylase, esterase, superoxide dismutase, catalas ...
- DOI:
- 10.1007/s10535-010-0133-9
- https://doi.org/10.1007/s10535-010-0133-9
- Author:
- Chandrika, M.; Ravishankar Rai, V.; Thoyajaksha
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 561-565
- ISSN:
- 0006-3134
- Subject:
- genetic stability; habitats; micropropagation; microsatellite repeats; plantlets
- Abstract:
- ... A DNA-based, inter simple sequence repeat (ISSR) markers were used to monitor genetic stability in micropropagated plantlets of Nothapodytes foetida. A total of 146 clear and distinct bands were produced using 26 primers resulting in 3 212 fragments. Out of 146, 135 bands (92.4 %) were monomorphic and 11 bands (7.53 %) were polymorphic which ranged from 200 to 21 226 bp in size. The number of band ...
- DOI:
- 10.1007/s10535-010-0100-5
- https://doi.org/10.1007/s10535-010-0100-5
- Author:
- Liu, Y. -H.; Huang, C. -J.; Chen, C. -Y.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 697-702
- ISSN:
- 0006-3134
- Subject:
- Bacillus cereus; Lilium formosanum; Northern blotting; disease severity; gene expression regulation; genes; leaf blight; leaves; photosystem II; sequence analysis; suppression subtractive hybridization; transcription (genetics)
- Abstract:
- ... Bacillus cereus C1L was demonstrated to induce systemic resistance in Lilium formosanum against leaf blight caused by Botrytis elliptica. Suppression subtractive hybridization library of L. formosanum triggered by B. cereus C1L were screened and 3 differentially expressed genes were identified. Based on sequence analysis, these genes encoding putative glycine-rich protein, metallothionein-like pro ...
- DOI:
- 10.1007/s10535-010-0123-y
- https://doi.org/10.1007/s10535-010-0123-y
74. In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants
- Author:
- Tyagi, P.; Khanduja, S.; Kothari, S.L.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 126-130
- ISSN:
- 0006-3134
- Subject:
- Capparis decidua; benzyladenine; explants; indole acetic acid; indole butyric acid; leaves; micropropagation; naphthaleneacetic acid; random amplified polymorphic DNA technique; shoots
- Abstract:
- ... A protocol for in vitro multiplication of Capparis decidua (Forsk.) Edgew. has been developed from cultured leaves procured from multiplying axillary shoots on the cultured nodal explants. The highest efficiency of shoot formation was observed on Murashige and Skoog (MS) medium containing 2 mg dm⁻³ benzyladenine (BA) and 0.5 mg dm⁻³ 1-naphthaleneacetic acid. The regenerated shoots were transferred ...
- DOI:
- 10.1007/s10535-010-0019-x
- https://doi.org/10.1007/s10535-010-0019-x
- Author:
- Grevenstuk, T.; Coelho, N.; Gonçalves, S.; Romano, A.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 391-394
- ISSN:
- 0006-3134
- Subject:
- Drosera; growth regulators; kinetin; micropropagation; plantlets; rooting; seedlings
- Abstract:
- ... A simple and efficient protocol for the micropropagation of Drosera intermedia, using cultures initiated from in vitro produced seedlings, is described. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher multiplication rates for ¼ MS (the lowest concentration), but was not affected by the addition of 0.1 mg dm⁻³ kinetin. In all ...
- DOI:
- 10.1007/s10535-010-0071-6
- https://doi.org/10.1007/s10535-010-0071-6
- Author:
- Bhat, M. A.; Mujib, A.; Junaid, A.; Mahmooduzzafar
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 757-760
- ISSN:
- 0006-3134
- Subject:
- Solanum nigrum; callus; flowering; in vitro regeneration; indole butyric acid; kinetin; leaves; roots; shoots
- Abstract:
- ... High frequency of shoot formation was achieved from Solanum nigrum L. leaves on Murashige and Skoog (MS) medium without any callusing stage. Shoot forming ability was more pronounced on leaves positioned dorsally. For shoot induction, 2.0 mg dm⁻³ benzylaminopurine and 1.5 mg dm⁻³ kinetin were observed to be the most effective plant growth regulators (PGRs). The present paper also describes first s ...
- DOI:
- 10.1007/s10535-010-0136-6
- https://doi.org/10.1007/s10535-010-0136-6
- Author:
- Alizadeh, M.; Singh, S. K.; Patel, V. B.; Bhattacharya, R. C.; Yadav, B. P.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 381-385
- ISSN:
- 0006-3134
- Subject:
- activated carbon; chlorophyll; culture media; genotype; grapes; indole butyric acid; potassium; proline; proteins; rooting; rootstocks; salt tolerance; sodium; sodium chloride; sugars
- Abstract:
- ... An investigation was undertaken to adjudge the in vitro salt tolerance and biochemical changes due to NaCl on four grape rootstocks (Dogridge, SO4, H-144 and 3309C). The in vitro two-node micro-cuttings from the established cultures were sub-cultured on rooting medium comprising Murashige and Skoog (MS) basal medium supplemented with 0.5 μM indolebutyric acid (IBA), 200 mg dm⁻³ activated charcoal ...
- DOI:
- 10.1007/s10535-010-0069-0
- https://doi.org/10.1007/s10535-010-0069-0
- Author:
- Dewir, Y. H.; Chakrabarty, D.; Lee, S. -H.; Hahn, E. -J.; Paek, K. -Y.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 357-360
- ISSN:
- 0006-3134
- Subject:
- Withania somnifera; benzyladenine; callus; explants; flavonoids; greenhouses; indole acetic acid; leaves; organogenesis; phenolic compounds; plant organs; plantlets; polysaccharides; roots; stems
- Abstract:
- ... The present study reports an efficient protocol for indirect shoot organogenesis and plantlets regeneration of Withania somnifera (L.) Dunal. Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). The highest callus induction rate (89.5 %) and shoot regeneration rate (92 ...
- DOI:
- 10.1007/s10535-010-0063-6
- https://doi.org/10.1007/s10535-010-0063-6
- Author:
- Gutiérrez-Carbajal, M. G.; Monforte-González, M.; Miranda-Ham, M.de L.; Godoy-Hernández, G.; Vázquez-Flota, F.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 430-434
- ISSN:
- 0006-3134
- Subject:
- Capsicum chinense; cell culture; elicitors; metabolism; methyl jasmonate; pepper; phenylalanine ammonia-lyase; salicylic acid; vanillin
- Abstract:
- ... Suspension cultures of Habanero pepper (Capsicum chinense Jacq.) were exposed to salicylic acid or methyl jasmonate to change secondary metabolism. Both treatments led to the accumulation of capsaicinoids and their late biosynthetic intermediate, vanillin. Both elicitors had a positive effect on the activities of phenylalanine ammonia lyase and coumarate O-methyltransferase, but none of them repre ...
- DOI:
- 10.1007/s10535-010-0078-z
- https://doi.org/10.1007/s10535-010-0078-z
- Author:
- Xu, L.; Najeeb, U.; Naeem, M. S.; Daud, M. K.; Cao, J. S.; Gong, H. J.; Shen, W. Q.; Zhou, W. J.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 659-663
- ISSN:
- 0006-3134
- Subject:
- Juncus effusus; cultivars; intercellular spaces; diploidy; flow cytometry; tetraploidy; colchicine; stomata; mesophyll; chlorophyll; chloroplasts; vascular bundles
- Abstract:
- ... Tetraploidy was induced in vitro in mat rush (Juncus effusus L.) cultivar Nonglin-4 by exposure to colchicine (0, 50, 100 and 500 mg dm⁻³) for 6, 12 and 24 h. Flow cytometric analysis was used to confirm the ploidy level. Anatomical and ultrastructural analyses at cellular and subcellular levels in tetraploid and diploid control plants revealed differences between diploid and tetraploid plants. Th ...
- DOI:
- 10.1007/s10535-010-0117-9
- https://doi.org/10.1007/s10535-010-0117-9
- Author:
- Robredo, A.; Pérez-López, U.; Lacuesta, M.; Mena-Petite, A.; Muñoz-Rueda, A.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 285-292
- ISSN:
- 0006-3134
- Subject:
- barley; carbon dioxide; carboxylation; drought; electron transfer; electrons; fluorescence; oxygen; phosphates; photosynthesis; soil; stomatal conductance; water content; water stress
- Abstract:
- ... We evaluated the combined effects of elevated CO₂ and water availability on photosynthesis in barley. Soil and plant water content decreased with water stress, but less under elevated CO₂ concentration (EC) compared with ambient CO₂ concentration (AC). During water stress, stomatal conductance, carboxylation rate, RuBP regeneration, and the rate of triose phosphate utilisation (TPU) were decreased ...
- DOI:
- 10.1007/s10535-010-0050-y
- https://doi.org/10.1007/s10535-010-0050-y
- Author:
- Ben Amar, A.; Cobanov, P.; Ghorbel, A.; Mliki, A.; Reustle, G. M.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 321-324
- ISSN:
- 0006-3134
- Subject:
- Cucurbita pepo; arabinogalactan proteins; arabinogalactans; callus; cell division; cell proliferation; cell suspension culture; chitinase; cultivars; culture media; growth promotion; immunoblotting; monoclonal antibodies; proteinases; zucchini
- Abstract:
- ... Arabinogalactan proteins (AGPs) secreted by zucchini squash (Cucurbita pepo L.) cell cultures into the medium are implicated in cell proliferation. Conditioned medium derived from cell suspensions of squash cultivar Dundoo could enhance multiplication rate of slow-growing cell line Cx3005. To examine the role of AGPs, a precipitation assay was performed using Yariv reagent which binds selectively ...
- DOI:
- 10.1007/s10535-010-0055-6
- https://doi.org/10.1007/s10535-010-0055-6
- Author:
- Ozbay, A.; Özgen, M.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 769-772
- ISSN:
- 0006-3134
- Subject:
- Triticum turgidum subsp. durum; callus; durum wheat; embryo culture; genotype; heterosis; hybrids; parents; soil; statistical analysis; vigor
- Abstract:
- ... The effect of heterosis on callus induction, callus mass and number of regenerated plants from mature embryo cultures of winter durum wheat (Triticum durum Desf.) hybrids was studied. A total of 14 F₁ hybrids and their parents were used for mature embryo culture. The statistical analysis of the results revealed that positive heterosis in callus mass was determined in only one F₁ hybrid and in numb ...
- DOI:
- 10.1007/s10535-010-0139-3
- https://doi.org/10.1007/s10535-010-0139-3
- Author:
- Wang, Y.; Zhang, Q. L.; Luo, Z. R.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 707-710
- ISSN:
- 0006-3134
- Subject:
- Diospyros kaki; complementary DNA; fruiting; fruits; gene expression; genes; genotype; open reading frames; persimmons; rapid amplification of cDNA ends
- Abstract:
- ... Leucoanthocyanidin reductase (LAR) converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon (Diospyros kaki Thunb.) fruits. A putative LAR gene (DkLAR) was isolated by rapid amplification of cDNA ends from young fruits. The full-length cDNA of DkLAR gene was 1 356 bp long and encoded an open reading frame of 349 residues. The deduced DkLAR protei ...
- DOI:
- 10.1007/s10535-010-0125-9
- https://doi.org/10.1007/s10535-010-0125-9
- Author:
- Jin, M. L.; Ahn, J. C.; Hwang, B.; Park, H. -S.; Lee, H. S.; Choi, D. -W.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 725-729
- ISSN:
- 0006-3134
- Subject:
- polypeptides; callus; emodin; biosynthesis; complementary DNA; active ingredients; recombinant proteins; expressed sequence tags; flowers; amino acid sequences; metabolites; leaves; Hypericum perforatum; hypericin; sequence homology; Hypericum erectum
- Abstract:
- ... Hypericin, a naphthodianthrone, has been identified as the principal active compound found in St. John's wort (Hypericum perforatum L.). To generate a gene resource for hypericin and other valuable metabolites, we generated expressed sequence tags (ESTs) from H. erectum. Analyses of the ESTs enabled us to select three cDNAs, HeHyp1, HeHyp2, and HeHyp3, evidencing significant sequence homology to H ...
- DOI:
- 10.1007/s10535-010-0129-5
- https://doi.org/10.1007/s10535-010-0129-5
86. Isolation of resistance gene analogues to powdery mildew resistance sequences in hexaploid wheat
- Author:
- Feng, D. S.; Ma, X.; Lin, A. L.; Wang, H. G.; Tian, J. C.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 551-555
- ISSN:
- 0006-3134
- Subject:
- stop codon; wheat; powdery mildew; amino acid sequences; Triticum aestivum; binding sites; hexaploidy; point mutation
- Abstract:
- ... This paper reports the characterization of the powdery mildew resistance homologous genes family of Triticum aestivum. Using degenerate primer pair for wheat resistance genes, we have cloned seven 3′ truncated powdery mildew resistance gene homologous fragments Tpc5a, Tp25a, Tp25b, Tp3a5a, Tp3a5b, Tp4b5a and Tp4b5b. These fragments were sequenced. The deduced amino acid sequences showed that six o ...
- DOI:
- 10.1007/s10535-010-0098-8
- https://doi.org/10.1007/s10535-010-0098-8
- Author:
- Di Baccio, D.; Minnocci, A.; Sebastiani, L.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 502-508
- ISSN:
- 0006-3134
- Subject:
- intercellular spaces; zinc; photosynthesis; Populus; mesophyll; stomata
- Abstract:
- ... In previous experiments elevated but sub-symptomatic applications of Zn (0.1 mM and 1 mM) caused impairments in growth parameters and photosynthetic performance of Populus × euramericana (Dode) Guinier clone I-214. The aim of this work was to evaluate leaf morphological and anatomical traits in this clone in response to the same Zn concentrations. The results showed that Zn treatments induced vari ...
- DOI:
- 10.1007/s10535-010-0088-x
- https://doi.org/10.1007/s10535-010-0088-x
88. Metabolic engineering using mtlD gene enhances tolerance to water deficit and salinity in sorghum
- Author:
- Maheswari, M.; Varalaxmi, Y.; Vijayalakshmi, A.; Yadav, S. K.; Sharmila, P.; Venkateswarlu, B.; Vanaja, M.; Pardha Saradhi, P.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 647-652
- ISSN:
- 0006-3134
- Subject:
- Escherichia coli; Sorghum bicolor; biochemical pathways; genetically modified organisms; germination; leaves; mannitol; metabolic engineering; polyethylene glycol; reverse transcriptase polymerase chain reaction; root growth; roots; salinity; shoots; sodium chloride; transgenes; water content
- Abstract:
- ... Sorghum bicolor L. Moench cv. SPV462 was transformed with the mtlD gene encoding for mannitol-1-phosphate dehydrogenase from E. coli with an aim to enhance tolerance to water deficit and NaCl stress. Transgene (pCAM mtlD) integration and expression were successfully confirmed by PCR, Southern, RT-PCR and Western analysis. Segregation analysis based on germination of T0 seed on hygromycin-supplemen ...
- DOI:
- 10.1007/s10535-010-0115-y
- https://doi.org/10.1007/s10535-010-0115-y
- Author:
- Jangid, R. K.; Sharma, R.; Sudarsan, Y.; Eapen, S.; Singh, G.; Purohit, A. K.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 703-706
- ISSN:
- 0006-3134
- Subject:
- DNA; Vigna aconitifolia; callus; explants; gene expression; leaves; microwave treatment; mutagenesis; nucleotide sequences; polypeptides; random amplified polymorphic DNA technique; seedlings
- Abstract:
- ... Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm⁻²) for 1, 3, 5 and 7 s before culturing. Callusing and shoot emergence from these explants were enhanced up to microwave exposure lasted 5 s while longer treatment of water-immersed explants delayed callusing. One polype ...
- DOI:
- 10.1007/s10535-010-0124-x
- https://doi.org/10.1007/s10535-010-0124-x
- Author:
- Kim, O. T.; Bang, K. H.; Jung, S. J.; Kim, Y. C.; Hyun, D. Y.; Kim, S. H.; Cha, S. W.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 47-53
- ISSN:
- 0006-3134
- Subject:
- Arabidopsis; Centella; Escherichia coli; Hevea; Panax ginseng; Southern blotting; amino acid sequences; complementary DNA; gene expression regulation; genes; ginsenosides; messenger RNA; methyl jasmonate; triterpenoid saponins
- Abstract:
- ... We isolated a gene encoding for farnesyl diphosphate synthase (FPS) from Panax ginseng, a species that produces a large quantity of triterpene saponins such as ginsenosides. The deduced amino acid sequence of PgFPS was 77, 84 and 95 % identical to those of Arabidopsis, Hevea, and Centella. Southern blot analysis indicated that P. ginseng contained more than two genes encoding for FPS. When the cDN ...
- DOI:
- 10.1007/s10535-010-0007-1
- https://doi.org/10.1007/s10535-010-0007-1
- Author:
- Lei, N.; Peng, S.; Niu, B.; Chen, J.; Zhou, J.; Tang, L.; Xu, Y.; Wang, S.; Chen, F.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 41-46
- ISSN:
- 0006-3134
- Subject:
- Davidia involucrata; amino acid sequences; amino acids; complementary DNA; fatty acid composition; genes; leaves; linoleic acid; molecular cloning; oleic acid; open reading frames; polyunsaturated fatty acids; reverse transcriptase polymerase chain reaction; sequence analysis; stems; temperature; tissues
- Abstract:
- ... In the conversion of oleic acid to linoleic acid, δ¹²-fatty acid desaturase (δ¹²-FAD) is involved. Based on the conserved oligo amino acid residues of the FAD2 genes from other plants, a new full-length cDNA (DiFAD2) encoding a δ¹²-FAD was cloned from Davidia involucrata Baill. Sequence analysis indicated that the DiFAD2 gene had an open reading frame (ORF) of 1 149 bp, coding for 382 amino acids ...
- DOI:
- 10.1007/s10535-010-0006-2
- https://doi.org/10.1007/s10535-010-0006-2
- Author:
- Subotić, A.; Trifunović, M.; Jevremović, S.; Petrić, M.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 592-596
- ISSN:
- 0006-3134
- Subject:
- 2,4-D; cell division; developmental stages; endangered species; leaves; plant growth substances; scanning electron microscopy; shoots; somatic embryogenesis; somatic embryos
- Abstract:
- ... Direct somatic embryogenesis of Frittilaria meleagris L. was induced using leaf base explants excised from in vitro grown shoots. Somatic embryos occurred at the basal part of leaf explants 4 weeks after culture on a Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or kinetin (KIN). The highest number of somatic embryos (SEs) were f ...
- DOI:
- 10.1007/s10535-010-0107-y
- https://doi.org/10.1007/s10535-010-0107-y
- Author:
- Wang, H. C.; Chen, J. T.; Chang, W. C.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 1-5
- ISSN:
- 0006-3134
- Subject:
- Areca catechu; apical meristems; callus; callus culture; dicamba; leaves; plantlets; scanning electron microscopy; somatic embryogenesis
- Abstract:
- ... Early morphogenetic events and repetitive embryogenesis from callus culture of betel nut palm (Areca catechu L.) were studied using scanning electron microscopy. On Murashige and Skoog (MS) medium supplemented with 2 mg dm⁻³ dicamba, callus culture has capacity to form plantlets via somatic embryogenesis and to form secondary embryos for about 4 years. However, various abnormal embryos without dif ...
- DOI:
- 10.1007/s10535-010-0001-7
- https://doi.org/10.1007/s10535-010-0001-7
- Author:
- Benelli, C.; Germanà, M. A.; Ganino, T.; Beghe', D.; Fabbri, A.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 224-230
- ISSN:
- 0006-3134
- Subject:
- Citrus reticulata; abscission; anther culture; developmental stages; leaves; mandarins; morphogenesis; plantlets; somatic embryogenesis; somatic embryos; stems
- Abstract:
- ... A morphological and anatomical study of regenerants obtained from mandarin anther culture was carried out. Beside morphologically normal somatic embryos, abnormal structures were originated in the course of somatic embryogenesis. Anatomical anomalies can be found at several growth stages, causing the formation of slender stems, stubby structures, non-functional leaves. When too long, some structur ...
- DOI:
- 10.1007/s10535-010-0040-0
- https://doi.org/10.1007/s10535-010-0040-0
- Author:
- Hu, X. J.; Zhang, Z. B.; Xu, P.; Fu, Z. Y.; Hu, S. B.; Song, W. Y.
- Source:
- Biologia plantarum 2010 v.54 no.2 pp. 213-223
- ISSN:
- 0006-3134
- Subject:
- drought; gene activation; genes; growth and development; heat stress; heat tolerance; induced resistance; mechanism of action; messenger RNA; nutrients; plant growth; proteins; rice; salinity; stress response; stress tolerance; transcription (genetics)
- Abstract:
- ... Unfavourable environment brings many kinds of stresses to plants. To survive such stresses, efficient resistance is required for the plants. Multifunctional genes enable the cross-talk among the various abiotic stress resistance systems. This paper reviews the action mechanisms of multifunctional genes. These genes can be classified into three groups: genes encoding diverse proteins through mRNA s ...
- DOI:
- 10.1007/s10535-010-0039-6
- https://doi.org/10.1007/s10535-010-0039-6
- Author:
- Vooková, B.; Machava, J.; Šalgovičová, A.; Kormuťák, A.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 177-180
- ISSN:
- 0006-3134
- Subject:
- Abies numidica; chlorophyll; cotyledons; growth regulators; seedlings; somatic embryos
- Abstract:
- ... The effect of maturation pretreatment on development and growth of Abies numidica De Lann. somatic embryos was studied. The most beneficial was pre-culturing on Schenk and Hildebrandt medium without growth regulator for 2 weeks. Dry mass accumulation of emblings was lower than that of seedlings after 50 d of culturing. Contents of microelements in seedlings were higher than in emblings, but macroe ...
- DOI:
- 10.1007/s10535-010-0030-2
- https://doi.org/10.1007/s10535-010-0030-2
- Author:
- Dibax, R.; Deschamps, C.; Bespalhok Filho, J. C.; Vieira, L. G. E.; Molinari, H. B. C.; De Campos, M. K. F.; Quoirin, M.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 6-12
- ISSN:
- 0006-3134
- Subject:
- Agrobacterium radiobacter; Eucalyptus saligna; Southern blotting; biosynthesis; callus; callus formation; cefotaxime; coculture; explants; genetic transformation; genotype; greenhouses; in vitro regeneration; kanamycin; leaves; organogenesis; polymerase chain reaction; proline; rooting; roots; shoots; transgenes
- Abstract:
- ... The purpose of this research was Eucalyptus saligna in vitro regeneration and transformation with P5CSF129A gene, which encodes Δ¹-pyrroline-5-carboxylate synthetase (P5CS), the key enzyme in proline biosynthesis. After selection of the most responsive genotype, shoot organogenesis was induced on leaf explants cultured on a callus induction medium (CI) followed by subculture on a shoot induction m ...
- DOI:
- 10.1007/s10535-010-0002-6
- https://doi.org/10.1007/s10535-010-0002-6
98. Over-expression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance
- Author:
- Xue, Y.; Peng, R.; Xiong, A.; Li, X.; Zha, D.; Yao, Q.
- Source:
- Biologia plantarum 2010 v.54 no.1 pp. 105-111
- ISSN:
- 0006-3134
- Subject:
- Arabidopsis thaliana; Saccharomyces cerevisiae; biochemical pathways; chlorophyll; electrical conductivity; fluorescence; gene overexpression; genes; heat shock proteins; heat stress; heat tolerance; malondialdehyde; proline; sugars; temperature; transgenic plants; yeasts
- Abstract:
- ... In the yeast Saccharomyces cerevisiae, the molecular chaperone HSP26 has the remarkable ability to sense increases in temperature directly and can switch from an inactive to a chaperone-active state. In this report, we analyzed the effect of expression of HSP26 in Arabidopsis thaliana plants and their response to high temperature stress. The hsp26 transgenic plants exhibited stronger growth than w ...
- DOI:
- 10.1007/s10535-010-0015-1
- https://doi.org/10.1007/s10535-010-0015-1
99. Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress
- Author:
- Sun, W. -H.; Duan, M.; Li, F.; Shu, D. -F.; Yang, S.; Meng, Q. -W.
- Source:
- Biologia plantarum 2010 v.54 no.4 pp. 614-620
- ISSN:
- 0006-3134
- Subject:
- RNA; ascorbate peroxidase; chloroplasts; cold stress; cold treatment; gels; gene overexpression; genes; heat stress; hydrogen peroxide; kanamycin; leaves; malondialdehyde; photosystem II; seed germination; temperature; tobacco; tomatoes; transgenic plants
- Abstract:
- ... In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtA ...
- DOI:
- 10.1007/s10535-010-0111-2
- https://doi.org/10.1007/s10535-010-0111-2
- Author:
- Cuesta, C.; Ordás, R. J.; Rodríguez, A.; Fernández, B.
- Source:
- Biologia plantarum 2010 v.54 no.3 pp. 435-442
- ISSN:
- 0006-3134
- Subject:
- DNA; Pinus pinea; amplified fragment length polymorphism; clones; genetic markers; genotype; micropropagation; microsatellite repeats; random amplified polymorphic DNA technique; somaclonal variation
- Abstract:
- ... Four different markers [random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and selective amplified microsatellite polymorphism length (SAMPL)] were applied for evaluating somaclonal variation of micropropagated genotypes of stone pine (Pinus pinea L.). The total number of primers tested was 130, with 223 combinations assayed ...
- DOI:
- 10.1007/s10535-010-0079-y
- https://doi.org/10.1007/s10535-010-0079-y