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You searched for: Journal Biologia plantarum Remove constraint Journal: Biologia plantarum Publication Year 2010 Remove constraint Publication Year: 2010
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1. 2D random walk representation of Begonia × tuberhybrida multiallelic loci used for germplasm identification

Author:
Wiesner, I.; Wiesnerová, D.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 353-356
ISSN:
0006-3134
Subject:
Begonia; DNA; alleles; genetic markers; genetic variation; germplasm; loci; nucleotide sequences; principal component analysis; sequence analysis
Abstract:
... In this study, we wanted to inspect whether the evolutionary driven differences in primary sequences could correlate, and thus predict the genetic diversity of related marker loci, which is an important criterion to assess the quality of any DNA marker. We adopted new approach of quantitative symbolic DNA sequence analysis called DNA random walk representation to study multiallelic marker loci fro ...
DOI:
10.1007/s10535-010-0062-7

2. ABA or cadmium induced phytochelatin synthesis in potato tubers

Author:
Stroiński, A.; Chadzinikolau, T.; Giżewska, K.; Zielezińska, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 117-120
ISSN:
0006-3134
Subject:
Solanum tuberosum; abscisic acid; cadmium; cysteine; glutathione; potatoes; tissues; tubers
Abstract:
... Short-term treatment of potato (Solanum tuberosum L.) tuber discs with CdCl₂ solution elevated both the StPCS1 transcript level, phytochelatin synthase (PCS) activity and contents of phytochelatin (PC) and abscisic acid (ABA). Similar effects but less marked were noticed after treatment of tuber tissues with ABA solution. Cd-treatment increased also cysteine (CYS) content but did not change glutat ...
DOI:
10.1007/s10535-010-0017-z

3. Acetylsalicylic acid ameliorates negative effects of NaCl or osmotic stress in Solanum stoloniferum in vitro

Author:
Daneshmand, F.; Arvin, M. J.; Kalantari, K. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 781-784
ISSN:
0006-3134
Subject:
electrolytes; ascorbate peroxidase; lipid peroxidation; drought; polyethylene glycol; potatoes; aspirin; hydrogen peroxide; explants; sodium; peroxidase; osmotic stress; linoleate 13S-lipoxygenase; superoxide dismutase; physiological response; potassium; sodium chloride; adverse effects; glutathione-disulfide reductase; catalase; salinity; Solanum stoloniferum
Abstract:
... The role of acetylsalicylic acid (0, 1 and 10 μM) pre-treatment in amelioration of salt and osmotic stress in a wild species of potato (Solanum stoloniferum) was investigated. We compared the effects of iso-osmotic concentrations of polyethylene glycol 6000 (15 %) and NaCl (80 mM) on the physiological responses of this species in explants grown in the liquid Murashige and Skoog medium. Both salt a ...
DOI:
10.1007/s10535-010-0142-8

4. Activities of antioxidant enzymes during strawberry fruit development and ripening

Author:
López, A. P.; Gochicoa, M. T. N.; Franco, A. R.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 349-352
ISSN:
0006-3134
Subject:
catalase; enzyme activity; fruiting; fruits; peroxidase; ripening; strawberries; superoxide dismutase
Abstract:
... The analyses of some antioxidant enzyme activities were carried out in the course of strawberry fruits development and ripening. The catalase activity was maximum in small-sized green fruits, it decreased in middle-sized green fruits and increased again during the ripening stages. The highest superoxide dismutase and peroxidase activities were observed in white fruits. ...
DOI:
10.1007/s10535-010-0061-8

5. Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus

Author:
Vatankhah, E.; Niknam, V.; Ebrahimzadeh, H.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 509-514
ISSN:
0006-3134
Subject:
Crocus sativus; callus; catalase; catechol oxidase; enzyme activity; kinetin; naphthaleneacetic acid; organogenesis; peroxidase; protein content; roots; shoots; sprouting; superoxide dismutase; thidiazuron
Abstract:
... The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm⁻³ 1-naphthaleneacetic acid (NAA) + 1.0 mg dm⁻³ thidiazuron and 1.0 mg dm⁻³ NAA + 2.0 mg dm⁻³ kinetin. For sprouting, regenerated shoot were subcultured on Mu ...
DOI:
10.1007/s10535-010-0089-9

6. Allelochemical effects on net nitrate uptake and plasma membrane H⁺-ATPase activity in maize seedlings

Author:
Abenavoli, M. R.; Lupini, A.; Oliva, S.; Sorgonà, A.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 149-153
ISSN:
0006-3134
Subject:
H-transporting ATP synthase; caffeic acid; corn; hydroponics; p-coumaric acid; plasma membrane; potassium nitrate; seedlings; umbelliferones
Abstract:
... Seven-day-old maize seedlings grown in a nitrogen-free hydroponic culture were exposed for 48 h to 0, 100 and 300 μM trans-cinnamic, p-coumaric, ferulic, caffeic acids, umbelliferone and 200 μM KNO₃. Net nitrate uptake was affected by trans-cinnamic, ferulic and p-coumaric acids in a concentration-dependent manner, and trans-cinnamic acid appeared to be the strongest inhibitor. Conversely, at low ...
DOI:
10.1007/s10535-010-0024-0

7. Alteration in growth and thylakoid membrane lipid composition of Azolla caroliniana under phosphate deficiency

Author:
Ismail, G. S. M.; Mohamed, H. E.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 671-676
ISSN:
0006-3134
Subject:
Azolla caroliniana; anthocyanins; biomass production; chlorophyll; fatty acid composition; ferns and fern allies; growth retardation; phosphates; phospholipids; phosphorus; photosystem II; thylakoids; ultrastructure
Abstract:
... The changes in the fresh biomass accumulation, photosynthetic and anthocyanin pigments, photosystem 2 (PS 2) activity, ultrastructure of chloroplast, total lipids and fatty acid composition of thylakoid membrane were followed in the aquatic fern Azolla caroliniana grown on medium either deficient or supplied with various phosphorus concentrations. The content of photosynthetic pigments and the ant ...
DOI:
10.1007/s10535-010-0119-7

8. Analysis of Lupinus albus heat-shock granule proteins in response to high temperature stress

Author:
Cherian, S.; Ferreira, R. B.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 587-591
ISSN:
0006-3134
Subject:
Lupinus albus; gene expression regulation; granules; heat; heat shock proteins; heat shock response; heat stress; molecular weight; two-dimensional gel electrophoresis
Abstract:
... An important aspect of heat-shock response of lupin (Lupinus albus cv. Rio Maior) is the formation of cytoplasmic granular aggregates, called heat-shock granules (HSGs). In this study, two-dimensional electrophoresis (2-DE) was used to detect the component proteins of HSG complexes formed in vivo. Evaluation of 2-DE revealed differential expression of several proteins under heat shock conditions w ...
DOI:
10.1007/s10535-010-0106-z

9. Analysis of the role of mitochondrial and endoplasmic reticulum localized small heat shock proteins in tomato

Author:
Nautiyal, P. C.; Shono, M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 715-719
ISSN:
0006-3134
Subject:
Solanum lycopersicum var. lycopersicum; biomass; cell membranes; endoplasmic reticulum; genetically modified organisms; heat; heat shock proteins; heat stress; mitochondria; photosynthesis; protein synthesis; temperature; tomatoes
Abstract:
... This communication examines the role of small heat shock proteins (sHsps) targeted to mitochondria (Mt) and endoplasmic reticulum (ER) in tomato plants (Lycopersicon esculentum Mill.) under heat stress. Genetic response of transgenic and wild type plants varied under optimum, moderately elevated and elevated temperature. In optimum temperature higher biomass was recorded in wild type than the tran ...
DOI:
10.1007/s10535-010-0127-7

10. Antioxidative enzymatic protection in leaves of two contrasting cowpea cultivars under salinity

Author:
Maia, J. M.; Costa de Macedo, C. E.; Voigt, E. L.; Freitas, J. B. S.; Silveira, J. A. G.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 159-163
ISSN:
0006-3134
Subject:
Vigna unguiculata; antioxidants; ascorbate peroxidase; cowpeas; cultivars; electrolytes; leaves; peroxidase; phenol; salinity; salt stress; salt tolerance; sodium; sodium chloride; superoxide dismutase; water content
Abstract:
... The aim of this work was to investigate the role of the antioxidant enzymes in salt tolerance comparing the salt-sensitive (Pérola) and a salt-tolerant (Pitiúba) cultivar of cowpea [Vigna unguiculata (L.) Walp.]. Salt stress (100 mM NaCl for 8 d) reduced the leaf growth rate more in the sensitive cultivar. The salt-induced decrease in the relative water content, Na⁺ accumulation and increase in le ...
DOI:
10.1007/s10535-010-0026-y

11. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources

Author:
Vuletić, M.; Hadži-Tašković Šukalović, V.; Marković, K.; Dragišić Maksimović, J.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 530-534
ISSN:
0006-3134
Subject:
Zea mays; ascorbate oxidase; ascorbate peroxidase; corn; enzyme activity; genotype; isoelectric focusing; nitrates; nitrogen; osmotic stress; peroxidase; phenolic compounds; polyethylene glycol; proline; roots; superoxide dismutase
Abstract:
... The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO₃ ⁻) or both nitrogen forms, nitrate and ammonium (NH₄ ⁺/NO₃ ⁻). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline ...
DOI:
10.1007/s10535-010-0093-0

12. Assessment of genetic diversity and relationships among Coix lacryma-jobi accessions using microsatellite markers

Author:
Ma, K. -H.; Kim, K. -H.; Dixit, A.; Chung, I. -M.; Gwag, J. -G.; Kim, T. -S.; Park, Y. -J.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 272-278
ISSN:
0006-3134
Subject:
Coix lacryma-jobi; algorithms; alleles; genetic background; genetic markers; genetic relationships; genetic variation; heterozygosity; loci; China; Korean Peninsula
Abstract:
... The present study describes the assessment of genetic diversity and relationships among 79 Job's tears (Coix lacrymajobi L.) accessions collected from China and Korea using 17 microsatellite markers. A total of 57 alleles were detected with an average of 3.4 alleles per locus. A high frequency of rare alleles (36.3 %) was observed within the collection. Values for observed (HO), expected heterozyg ...
DOI:
10.1007/s10535-010-0047-6

13. CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng

Author:
Kiselev, K. V.; Grishchenko, O. V.; Zhuravlev, Y. N.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 621-630
ISSN:
0006-3134
Subject:
Panax ginseng; cell culture; gene expression; genes; protein kinases; proteins; salt stress; salt tolerance; sodium chloride
Abstract:
... CDPKs (calcium-depended protein kinases) are of great importance for the activation of defense reactions in plants. In this study, we aimed to find a connection between CDPK expression and increased salt tolerance in Panax ginseng. Treatment of P. ginseng cell cultures with W7 (CDPK protein inhibitor) showed that CDPK proteins were necessary for salt tolerance. Expression of PgCDPK1c, PgCDPK2c and ...
DOI:
10.1007/s10535-010-0112-1

14. Carbon nutrition of mature green orchid Serapias strictiflora and its mycorrhizal fungus Epulorhiza sp

Author:
Látalová, K.; Baláž, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 97-104
ISSN:
0006-3134
Subject:
Orchidaceae; autotrophs; carbon; corn; mycelium; mycorrhizal fungi; nutrition; organic compounds; roots; saprotrophs; tubers
Abstract:
... We studied the nutritional modes of the orchid Serapias strictiflora and its mycorrhizal fungus Epulorhiza sp. using the differences in carbon isotopic composition (δ¹³C) of C₃ orchid and C₄ maize tissues. We found that if cultivated in substrate lacking any organic compounds, the mycorrhizal extraradical mycelia (δ¹³C = −26.3 ± 0.2 ‰) developed well, despite being fully dependent on nutrition fro ...
DOI:
10.1007/s10535-010-0014-2

15. Changes in AFLP and SSR DNA polymorphisms induced by short-term space flight of rice seeds

Author:
Lu, J. Y.; Zhang, W. L.; Xue, H.; Pan, Y.; Zhang, C. H.; He, X. H.; Liu, M.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 112-116
ISSN:
0006-3134
Subject:
DNA; DNA primers; Oryza sativa; amplified fragment length polymorphism; flight; genetic polymorphism; leaves; microsatellite repeats; rice; seeds; space flight
Abstract:
... Differences of both amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) polymorphisms were compared between the 60-d-old rice (Oryza sativa L. cv. DH7) and F3 rice plants (SP3) derived from seed, which endured a 7-d-space flight in March 2002. Total leaf AFLP DNA bands amplified from 22 primer pairs were 537 in DH7, whereas 562 in SP3. From the total 267 SSR DNA bands ge ...
DOI:
10.1007/s10535-010-0016-0

16. Changes in biomass and photosynthetic parameters of tomato plants exposed to trivalent and hexavalent chromium

Author:
Henriques, F. S.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 583-586
ISSN:
0006-3134
Subject:
biomass; chlorophyll; chromium; culture media; fluorescence; leaves; photosynthesis; plant growth; plant tissues; roots; stomatal conductance; tomatoes; toxicity
Abstract:
... Tomato plants were treated for two weeks with different concentrations of Cr(III) or Cr(VI) compounds to compare their toxic effects. The concentration of total Cr in plant tissues increased linearly with its concentration in the growth medium and Cr accumulated largely in the roots, regardless of the form in which it was supplied to the plant. All measured plant growth parameters were negatively ...
DOI:
10.1007/s10535-010-0105-0

17. Characteristics of chlorophyll fluorescence and antioxidative system in super-hybrid rice and its parental cultivars under chilling stress

Author:
Zhang, Y. H.; Chen, L. J.; He, J. L.; Qian, L. S.; Wu, L. Q.; Wang, R. F.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 164-168
ISSN:
0006-3134
Subject:
Oryza sativa; ascorbate peroxidase; chlorophyll; cold tolerance; cooling; cultivars; fluorescence; genotype; glutathione-disulfide reductase; photosystem II; rice; superoxide dismutase
Abstract:
... Characteristics of chlorophyll fluorescence and antioxidative system were investigated in rice (Oryza sativa L.) super-hybrid Liangyoupeijiu (LYPJ), maternal cultivar Peiai64s, and paternal cultivar indica rice 9311 under chilling stress. During 6-d chilling treatment, chlorophyll content of all three genotypes was gradually declined. However, the decrease in photosystem 2 (PS 2) maximum photochem ...
DOI:
10.1007/s10535-010-0027-x

18. Characterization of a xylanase inhibitor TAXI-I from wheat

Author:
Weng, X. -Y.; Huang, Y. -Y.; Gao, H.; Sun, J. -Y.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 154-158
ISSN:
0006-3134
Subject:
Aspergillus niger; Bacillus subtilis; Escherichia coli; Thermobifida fusca; Triticum aestivum; bacteria; fungi; gene expression; genes; gibberellic acid; glycosides; hybrids; methyl jasmonate; parents; wheat; xylanases
Abstract:
... Xylanase inhibitor TAXI-I gene was cloned from wheat (Triticum aestivum L.) and then TAXI-I encoding sequence was expressed in Escherichia coli. The recombinant TAXI-I protein inhibited glycoside hydrolase (GH) family 11 xylanases in Aspergillus niger (Anx; a fungal xylanase), and Thermomonospora fusca (Tfx; a bacterial xylanase), and also inhibited hybrid xylanases Atx (a hybrid xylanase whose pa ...
DOI:
10.1007/s10535-010-0025-z

19. Characterization of the antioxidant system during the vegetative development of pea plants

Author:
Díaz-Vivancos, P.; Barba-Espín, G.; Clemente-Moreno, M. J.; Hernández, J. A.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 76-82
ISSN:
0006-3134
Subject:
antioxidants; ascorbate oxidase; ascorbate peroxidase; catalase; enzyme activity; glutathione; glutathione dehydrogenase (ascorbate); monodehydroascorbate reductase (NADH); peas; peroxidase; roots; seedlings; shoots; superoxide dismutase; tissues
Abstract:
... The antioxidative system was studied during the development of pea plants. The reduced glutathione (GSH) content was higher in shoots than in roots, but a greater redox state of glutathione existed in roots compared with shoots, at least after 7 d of growth. The 3-d-old seedlings showed the highest content of oxidised ascorbate (DHA), which correlated with the ascorbate oxidase (AAO) activity. Als ...
DOI:
10.1007/s10535-010-0011-5

20. Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances

Author:
Osório, M. L.; Osório, J.; Romano, A.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 415-422
ISSN:
0006-3134
Subject:
Rhododendron ponticum; acclimation; chlorophyll; electron transfer; energy; fluorescence; light intensity; micropropagation; photoinhibition
Abstract:
... The aim of this study was to investigate acclimation of micropropagated plants of Rhododendron ponticum subsp. baeticum to different irradiances and recovery after exposure to high irradiance. Plants grown under high (HL) or intermediate (IL) irradiances displayed higher values of maximum electron transport rate (ETRmax) and light saturation coefficient (Ek) than plants grown under low irradiance ...
DOI:
10.1007/s10535-010-0076-1

21. Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis

Author:
Lu, X.; Zhou, W.; Gao, F.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 798-800
ISSN:
0006-3134
Subject:
Citrus sinensis; anthocyanins; biosynthesis; chromosomes; fluorescence in situ hybridization; genes; karyotyping; metaphase; oranges; ribosomal DNA
Abstract:
... Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S r ...
DOI:
10.1007/s10535-010-0146-4

22. Citrate transporters play an important role in regulating aluminum-induced citrate secretion in Glycine max

Author:
You, J. F.; Hou, N. N.; Xu, M. Y.; Zhang, H. M.; Yang, Z. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 766-768
ISSN:
0006-3134
Subject:
Glycine max; aluminum; citrates; cultivars; cycloheximide; exudation; mitochondrial membrane; plasma membrane; protein synthesis; protein synthesis inhibitors; pyridoxal; roots; secretion; soybeans; transporters
Abstract:
... To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor, anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated after 4 h of Al ...
DOI:
10.1007/s10535-010-0138-4

23. Clonal propagation of Zephyranthes grandiflora using bulbs as explants

Author:
Gangopadhyay, M.; Chakraborty, D.; Dewanjee, S.; Bhattacharya, S.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 793-797
ISSN:
0006-3134
Subject:
DNA; Zephyranthes; bulbs; carbendazim; explants; genomics; gibberellic acid; indole butyric acid; mercuric chloride; microbial contamination; micropropagation; microsatellite repeats; ornamental plants; polymerase chain reaction; roots; shoots; sucrose
Abstract:
... Zephyr lily (Zephyranthes grandiflora), an important ornamental plant has been micropropagated in vitro after controlling microbial contamination by a pretreatment with 0.2 % Bavistin and 0.1 % Pantomycin for 4 h before final sterilization with 0.1 % mercuric chloride. In 67 % of the sterile cultures, 11 shoots on average were regenerated directly from basal half of bulb scales in Murashige and Sk ...
DOI:
10.1007/s10535-010-0145-5

24. Cloning and characterization of two osmotin isoforms from Piper colubrinum

Author:
Mani, T.; Manjula, S.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 377-380
ISSN:
0006-3134
Subject:
Piper; antifungal properties; black pepper; breeding; cDNA libraries; complementary DNA; genes; point mutation
Abstract:
... In the present study, we report the cloning and sequence characterization of two isoforms of osmotin, an antifungal PR-5 gene homologue, from a salicylic acid-induced subtracted cDNA library earlier generated in Piper colubrinum. The larger form of the gene is 693 bp long, encoding a 21.5 kDa protein. The smaller form comprises a 543 bp long coding sequence which code for a protein of 16.4 kDa. A ...
DOI:
10.1007/s10535-010-0068-1

25. Cold tolerance, water relations and accumulation of osmolytes in Bituminaria bituminosa

Author:
Walker, D. J.; Romero, P.; Correal, E.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 293-298
ISSN:
0006-3134
Subject:
Bituminaria bituminosa; cold; cold tolerance; electrolytes; freezing; leaves; osmotic pressure; osmotolerance; proline; shrubs; spring; sugars; temperature; winter
Abstract:
... We determined the cold (freezing) tolerance of five Spanish populations of the perennial shrub Bituminaria bituminosa (L.) C.H. Stirton (Fabaceae), as the temperature at which 50 % of leaf electrolytes are released (LT₅₀) using leaves of field-grown plants, obtained in two winters and one spring. The freezing tolerance was greater in winter and reflected the minimum temperatures at the original si ...
DOI:
10.1007/s10535-010-0051-x

26. Comparison of three genetic similarity coefficients based on dominant markers from predominantly self-pollinating species

Author:
Beharav, A.; Maras, M.; Kitner, M.; Šuštar-Vozlič, J.; Sun, G. L.; Doležalová, I.; Lebeda, A.; Meglič, V.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 54-60
ISSN:
0006-3134
Subject:
DNA; Lactuca saligna; Phaseolus vulgaris; Triticum turgidum subsp. dicoccoides; amplified fragment length polymorphism; beans; genetic variation; lettuce; population structure; random amplified polymorphic DNA technique; self-pollination
Abstract:
... Three genetic similarity coefficients were estimated and compared for their usefulness: simple matching (S SM), Jaccard's (S J) and Dice's (S D), all based on dominant markers data from individuals representing predominantly self-pollinating species. AFLP markers were used to analyze 139 Phaseolus vulgaris L. (common bean) and 67 Lactuca saligna L. (least lettuce) accessions, and RAPD markers were ...
DOI:
10.1007/s10535-010-0008-0

27. Concurrent occurrence of α-amylase inhibitor and stimulator in red kidney bean seed: physiological implications

Author:
Alizadeh, H.; Leung, D. W. M.; Cole, A. L. J.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 195-197
ISSN:
0006-3134
Subject:
phytohemagglutinin; kidney beans; cotyledons; Phaseolus vulgaris; alpha-amylase
Abstract:
... It is hypothesized that since protein α-amylase inhibitor (α-AI) and stimulator might be present together in red kidney bean (Phaseolus vulgaris L.) seeds, their in vitro interactions might influence their detection and quantification. Assay of α-AI using extracts from the embryonic axes revealed an unexpected finding in that the extracts stimulated rather than inhibited α-amylase activity. The co ...
DOI:
10.1007/s10535-010-0034-y

28. Contents of polyamines during vernalization in wheat and the effect of zearalenone

Author:
Filek, M.; Biesaga-Kościelniak, J.; Marcińska, I.; Cvikrová, M.; Macháčková, I.; Krekule, J.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 483-487
ISSN:
0006-3134
Subject:
cold treatment; germination; leaves; putrescine; roots; seedlings; spermidine; vernalization; winter wheat; zearalenone
Abstract:
... The contents of endogenous free and conjugated polyamines, putrescine (Put) and spermidine (Spd), were determined during 9 week of vernalization (at 5 °C) in winter wheat seedlings cultivated on Murashige and Skoog media without (MS0) and with 2 mg dm⁻³ zearalenone (MSZEN). At the 4th week of chilling treatment, which is sufficient to induce generative development in 30 % of plants, the marked inc ...
DOI:
10.1007/s10535-010-0085-0

29. Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization

Author:
Mirzaghaderi, G.; Karimzadeh, G.; Hassani, H. S.; Jalali-Javaran, M.; Baghizadeh, A.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 252-258
ISSN:
0006-3134
Subject:
chromosome banding; chromosome number; chromosomes; crossing; cultivars; cytogenetic analysis; genotype; hybrids; in situ hybridization; meiosis; salt tolerance; seeds; wheat
Abstract:
... The new salt tolerant cereal, Tritipyrum (2n=6x=42, AABBEbEb) offers potential to introduce desirable characters for wheat improvements. This study was aimed to generate a segregating population from Iranian local wheat cultivars (2n=6x=42, AABBDD) and Tritipyrum crosses, study of the meiotic behaviour in F₂ hybrids and identification of Eb chromosomes in F₃ individuals. Results showed meiotic abn ...
DOI:
10.1007/s10535-010-0044-9

30. Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber

Author:
Diao, W. -P.; Bao, S. -Y.; Jiang, B.; Cui, L.; Qian, C. -T.; Chen, J. -F.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 373-376
ISSN:
0006-3134
Subject:
Cucumis sativus; anaphase; chromosome segregation; chromosomes; cucumbers; cytokinesis; gametophytes; germination; meiosis; metaphase; pollen; telophase
Abstract:
... With improved staining and chromosome preparation techniques, meiosis of pollen mother cells (PMCs) and male gametophyte development in autotetraploid cucumber (Cucumis sativus L.) was studied to understand the correlation between chromosomes behaviour and fertility. Various chromosome configurations, e.g. multivalent, quadrivalents, trivalents, bivalents and univalents were observed in most PMCs ...
DOI:
10.1007/s10535-010-0067-2

31. Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Author:
Kumar, P.; Singhal, V. K.; Kaur, D.; Kaur, S.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 181-184
ISSN:
0006-3134
Subject:
Clematis; chromatin; chromosome number; meiosis; pollen; prophase; stickiness; tetraploidy; India
Abstract:
... Present cytological investigations from the cold desert regions of Lahaul-Spiti and Kinnaur (India) record the first ever tetraploid (2n=32) chromosome count and cytomixis in Clematis orientalis L. var. acutifolia Hook. f. et Thoms. The phenomenon of cytomixis (9.33-29.80 %) involving chromatin transfer among 2-3 proximate pollen mother cells (PMCs) during male meiosis occurs through narrow and br ...
DOI:
10.1007/s10535-010-0031-1

32. DIANTHIN, a negative selection marker in tobacco, is non-toxic in transgenic rice and confers sheath blight resistance

Author:
Shah, J. M.; Veluthambi, K.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 443-450
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Dianthus caryophyllus; Mungbean yellow mosaic virus; Oryza sativa; blight; callus; gene expression; genes; leaves; promoter regions; rice; tobacco; toxicity; transgenic plants
Abstract:
... The DIANTHIN gene encoding a ribosome-inactivating protein (RIP) from Dianthus caryophyllus L. was tested for negative selection in tobacco and rice. Tobacco leaf discs and scutellum-derived callus of rice were transformed with Agrobacterium tumefaciens strain LBA4404 (pSB1, pJAS1). pJAS1 harbors the DIANTHIN gene under the control of the CaMV 35S promoter. Tobacco transformation efficiency, in co ...
DOI:
10.1007/s10535-010-0080-5

33. Development of AFLP and STS markers linked to a waterlogging tolerance in Korean soybean landraces

Author:
Kang, S. -Y.; Lee, K. J.; Lee, G. -J.; Kim, J. -B.; Chung, S. -J.; Song, J. Y.; Lee, B. -M.; Kim, D. S.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 61-68
ISSN:
0006-3134
Subject:
DNA primers; Glycine max; amplified fragment length polymorphism; chlorophyll; cluster analysis; flooded conditions; gamma radiation; genotype; glucose; landraces; malondialdehyde; marker-assisted selection; mutants; mutation; soybeans
Abstract:
... Among the 400 soybean (Glycine max) landraces, we selected 3 tolerant (KAS150-9, KAS160-15, and KAS170-9) and 3 susceptible lines (KAS160-14, KAS160-20, and KAS201-6-1) by the survival percentage and injury scores. Susceptible lines showed decrease in chlorophyll content and increase in glucose and malondialdehyde (MDA) contents under waterlogging stress, while tolerant lines did not change signif ...
DOI:
10.1007/s10535-010-0009-z

34. Direct plant regeneration from encapsulated nodal segments of Vitex negundo

Author:
Ahmad, N.; Anis, M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 748-752
ISSN:
0006-3134
Subject:
Vitex negundo; acetic acid; artificial seeds; calcium chloride; concentration of production; culture media; encapsulation; germination; kinetin; naphthaleneacetic acid; plantlets; sodium alginate
Abstract:
... An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na₂-alginate with 100 mM CaCl₂ has been found to be optimum concentration for the production of uniform synthetic ...
DOI:
10.1007/s10535-010-0134-8

35. Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

Author:
Khurana-Kaul, V.; Kachhwaha, S.; Kothari, S. L.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 369-372
ISSN:
0006-3134
Subject:
Jatropha curcas; benzyladenine; buds; callus; callus formation; copper sulfate; explants; gibberellic acid; indole butyric acid; leaves; plantlets; shoots; thidiazuron
Abstract:
... Leaf explants of Jatropha curcas cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ; 0.90 μM) in combination with indole-3-butyric acid (IBA; 0.98μM) produced adventitious shoot buds directly on the surface of the explants without formation of intervening callus while shoot bud formation was accompanied with callus formation on medium supplemented with 6-benzylaminopu ...
DOI:
10.1007/s10535-010-0066-3

36. Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

Author:
Kiran Ghanti, S.; Sujata, K. G.; Srinath Rao, M.; Kavi Kishor, P. B.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 121-125
ISSN:
0006-3134
Subject:
2,4,5-T; 2,4-D; Cicer arietinum; abscisic acid; chickpeas; cotyledons; cultivars; developmental stages; explants; kinetin; naphthaleneacetic acid; picloram; plantlets; somatic embryogenesis; somatic embryos
Abstract:
... A protocol for plant regeneration via somatic embryogenesis was developed in two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri. Somatic embryos were induced from immature cotyledons on Murashige and Skoog's (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-naphthaleneacetic acid (NAA) and ...
DOI:
10.1007/s10535-010-0018-y

37. Direct somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum

Author:
Ma, G. H.; He, C. X.; Ren, H.; Zhang, Q. M.; Li, S. J.; Zhang, X. H.; Eric, B.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 361-365
ISSN:
0006-3134
Subject:
acclimation; activated carbon; adventitious roots; adventitious shoots; benzyladenine; cytokinins; endangered species; explants; indole butyric acid; kinetin; leaves; organogenesis; pH; plantlets; rooting; somatic embryogenesis; somatic embryos; thidiazuron
Abstract:
... An efficient propagation system via somatic embryogenesis and shoot organogenesis and plant regeneration system for endangered species Primulina tabacum Hance was established. Thidiazuron (TDZ) was the key plant growth regulator for inducing somatic embryogenesis and kinetin (KIN) and 6-benzylaminopurine (BAP) were the key cytokinins for inducing shoot organogenesis from leaf explants. TDZ combine ...
DOI:
10.1007/s10535-010-0064-5

38. An EST-SSR marker linked with yellow rust resistance in wheat

Author:
Ercan, S.; Ertugrul, F.; Aydin, Y.; Akfirat, F. S.; Hasancebi, S.; Akan, K.; Mert, Z.; Bolat, N.; Yorgancilar, O.; Altinkut-Uncuoglu, A.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 691-696
ISSN:
0006-3134
Subject:
DNA; Triticum aestivum; expressed sequence tags; genetic markers; genotype; hybrids; microsatellite repeats; seedlings; stripe rust; wheat
Abstract:
... Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F₂ seedlings were constructed. 78 EST-SSR prime ...
DOI:
10.1007/s10535-010-0122-z

39. Ectopic over-expression of two apple Flowering Locus T homologues, MdFT1 and MdFT2, reduces juvenile phase in Arabidopsis

Author:
Li, W. M.; Tao, Y.; Yao, Y. X.; Hao, Y. J.; You, C. X.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 639-646
ISSN:
0006-3134
Subject:
Arabidopsis; Malus domestica; amino acid sequences; amino acids; apples; complementary DNA; flowering; gene overexpression; genes; leaves; loci; open reading frames; reverse transcriptase polymerase chain reaction
Abstract:
... To get insight into mechanism by which apple tree (Malus domestica Borkh.) regulates flowering, two apple flowering locus T (FT) homologues, MdFT1 and MdFT2, were isolated from the leaf cDNAs of cultivar Gala. The open reading frames (ORFs) of two MdFTs encoded 174 amino acids. The deduced amino acid sequence of MdFT1 and MdFT2 showed 94.3 % similarity to each other, while 72.6 and 76.0 % to AtFT ...
DOI:
10.1007/s10535-010-0114-z

40. Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants

Author:
Mýtinová, Z.; Motyka, V.; Haisel, D.; Gaudinová, A.; Lubovská, Z.; Wilhelmová, N.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 461-470
ISSN:
0006-3134
Subject:
Arabidopsis thaliana; Nicotiana tabacum; abscisic acid; antioxidants; ascorbate peroxidase; catalase; cytokinins; enzyme activity; genes; glutathione-disulfide reductase; growth retardation; indole acetic acid; leaves; phenotype; promoter regions; roots; salinity; superoxide dismutase; tobacco; transgenic plants; water stress; zinc
Abstract:
... The responses of antioxidant enzymes (AOE) ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in soluble protein extracts from leaves and roots of tobacco (Nicotiana tabacum L. cv. Samsun NN) plants to the drought stress, salinity and enhanced zinc concentration were investigated. The studied tobacco included wild-type (WT) and transgenic plants ...
DOI:
10.1007/s10535-010-0082-3

41. Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis

Author:
Ding, W.; Song, L.; Wang, X.; Bi, Y.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 607-613
ISSN:
0006-3134
Subject:
Phragmites australis; abscisic acid; ascorbate peroxidase; callus; catalase; cell viability; ecotypes; fluridone; growth retardation; heat stress; heat tolerance; hydrogen peroxide; malondialdehyde; peroxidase; stress tolerance; superoxide dismutase; swamps
Abstract:
... Dune reed (DR) and swamp reed (SR) are two ecotypes of reed (Phragmites communis Trin.) that displayed differences in stress tolerance. To uncover the molecular basis for such difference, the effects of heat stress were studied using the calli derived from the two ecotypes. Heat stress caused increased ion leakage, inhibited growth, decreased cell viability, and elevated hydrogen peroxide (H₂O₂) a ...
DOI:
10.1007/s10535-010-0110-3

42. Effect of gibberellic acid combined with saponin on shoot elongation of Asparagus officinalis

Author:
Saharan, V.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 740-742
ISSN:
0006-3134
Subject:
Asparagus officinalis; culture media; gibberellic acid; saponins; shoots; tissue culture
Abstract:
... Effect of gibberellic acid (GA₃) combined with saponin on shoot elongation of Asparagus officinalis was evaluated in tissue culture. Addition of saponin to GA₃ supplemented Murashige and Skoog (MS) basal medium showed a dose depended effect on shoot length of Asparagus officinalis. However, increasing concentration of saponin above 3.0 mg dm⁻³ decreased shoot length and showed yellowing and thinni ...
DOI:
10.1007/s10535-010-0132-x

43. Effect of photoperiod during growth of Chenopodium rubrum mother plants on properties of offspring

Author:
Mitrović, A.; Bogdanović, J.; Giba, Z.; Ćulafić, L.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 735-739
ISSN:
0006-3134
Subject:
Chenopodium rubrum; flowering; in vitro culture; mothers; photoperiod; progeny; seed germination; seed maturation
Abstract:
... Using in vitro culture, we determined the effect of photoperiod during growth of Chenopodium rubrum mother plants on vegetative and reproductive development of offspring. Photoperiod during flowering induction of mother plants (the first 6 d after the germination) has the key influence on seed germination and offspring growth, while offspring flowering and seed maturation is determined by photoper ...
DOI:
10.1007/s10535-010-0131-y

44. Effect of salicylic acid pretreatment on cadmium toxicity in wheat

Author:
Moussa, H. R.; El-Gamal, S. M.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 315-320
ISSN:
0006-3134
Subject:
Triticum aestivum; abscisic acid; biomass; cadmium; chlorophyll; chloroplasts; hydrogen peroxide; malondialdehyde; phosphoenolpyruvate carboxylase; proline; root growth; roots; salicylic acid; seeds; toxicity; ultrastructure; water content; wheat
Abstract:
... Cadmium (100, 400 and 1000 μM CdCl₂) treatments resulted in the inhibition of root dry biomass, root elongation and increased Cd accumulation in wheat (Triticum aestivum L.) roots. Further, these treatments decreased relative water content, chlorophyll content, ¹⁴CO₂-fixation, activities of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase and abscisic acid content while in ...
DOI:
10.1007/s10535-010-0054-7

45. Effect of temperature on water transport through aquaporins

Author:
Ionenko, I. F.; Anisimov, A. V.; Dautova, N. R.
Source:
Biologia plantarum 2010 v.54 no.3 pp. 488-494
ISSN:
0006-3134
Subject:
activation energy; aquaporins; corn; lipid bilayers; magnetic fields; mercuric chloride; nuclear magnetic resonance spectroscopy; surface area; water temperature
Abstract:
... The mean effective water self-diffusion coefficient in maize root segments under the effect of aquaporin blocker (mercuric chloride, 0.1 mM) was measured using the spin-echo NMR method with pulsed magnetic field gradient within the temperature range from 10 to 35 °C. HgCl₂ caused the reduction in water diffusion by 30 % as compared to the control samples. Temperature dependences of water self-diff ...
DOI:
10.1007/s10535-010-0086-z

46. Effect of zinc and cadmium on physiological and production characteristics in Matricaria recutita

Author:
Kummerová, M.; Zezulka, Š.; Kráľová, K.; Masarovičová, E.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 308-314
ISSN:
0006-3134
Subject:
Matricaria chamomilla; cadmium; carotenoids; chamomile; chlorophyll; fluorescence; hydroponics; pigments; respiratory rate; roots; shoots; zinc
Abstract:
... Effects of zinc (12-180 μM) alone and in mixtures with 12 μM Cd on metal accumulation, dry masses of roots and shoots, root respiration rate, variable to maximum fluorescence ratio (FV/FM), and content of photosynthetic pigments were studied in hydroponically cultivated chamomile (Matricaria recutita) plants. The content of Zn in roots and shoots increased with the increasing external Zn concentra ...
DOI:
10.1007/s10535-010-0053-8

47. Effects of exogenous spermine on sweet sorghum during germination under salinity

Author:
Chai, Y. Y.; Jiang, C. D.; Shi, L.; Shi, T. S.; Gu, W. B.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 145-148
ISSN:
0006-3134
Subject:
Sorghum bicolor; catalase; germination; glutathione-disulfide reductase; growth retardation; malondialdehyde; peroxidase; proline; protein content; roots; salinity; seedlings; shoots; sodium chloride; spermine; sweet sorghum
Abstract:
... Seedlings of Sorghum bicolor (L.) Moench were subjected to 180 mM NaCl with or without 0.25 mM spermine (SPM) for 7 d. NaCl treatment resulted in the inhibition of growth and increased the content of free proline, soluble protein and malondialdehyde (MDA). Additionally, it also enhanced the activity of catalase (CAT), peroxidase (POX) in both shoots and roots, while decreased that of glutathione r ...
DOI:
10.1007/s10535-010-0023-1

48. Efficient biolistic transformation of the moss Physcomitrella patens

Author:
Šmídková, M.; Holá, M.; Angelis, K. J.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 777-780
ISSN:
0006-3134
Subject:
Physcomitrella patens; biolistics; gold; homologous recombination; mosses and liverworts; plasmids; transgenes
Abstract:
... High rates of homologous recombination (HR) in comparison to other plants make the moss Physcomitrella patens an attractive model organism for genetic studies as well as biotechnological applications. We describe a simple protocol for the efficient biolistic transformation of protonemal tissue with minimum tissue handling steps. The transformation efficiency depends on the biolistic conditions. Th ...
DOI:
10.1007/s10535-010-0141-9

49. Efficient in vitro plant regeneration from shoot apices and gene transfer by particle bombardment in Jatropha curcas

Author:
Purkayastha, J.; Sugla, T.; Paul, A.; Solleti, S. K.; Mazumdar, P.; Basu, A.; Mohommad, A.; Ahmed, Z.; Sahoo, L.
Source:
Biologia plantarum 2010 v.54 no.1 pp. 13-20
ISSN:
0006-3134
Subject:
Cauliflower mosaic virus; Jatropha curcas; Southern blotting; beta-glucuronidase; culture media; explants; gene transfer; genetic transformation; gibberellic acid; gold; kanamycin; osmotic treatment; particle size; plant establishment; plantlets; plasmids; polymerase chain reaction; shoots; transgenes; transgenic plants
Abstract:
... An efficient and reproducible in vitro plant regeneration system from shoot apices was developed in Jatropha curcas. Benzylaminopurine (BAP; 2.5 μM) was most effective in inducing an average of 6.2 shoots per shoot apex. Incorporation of gibberellic acid (GA3; 0.5 μM) to basal medium was found essential for elongation of shoots. The BAP-habituated mother explants continuously produced shoots durin ...
DOI:
10.1007/s10535-010-0003-5

50. Efficient production of transgenic tomatoes via Agrobacterium-mediated transformation

Author:
Kaur, P.; Bansal, K. C.
Source:
Biologia plantarum 2010 v.54 no.2 pp. 344-348
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Solanum lycopersicum var. lycopersicum; Southern blotting; flowers; fruits; gene expression; indole acetic acid; inorganic salts; kanamycin; leaves; neomycin; reverse transcriptase polymerase chain reaction; shoots; tomatoes; transgenes; transgenic plants; vitamins; zeatin
Abstract:
... Cotyledonary leaves of 9-d-old tomato (Lycopersicon esculentum Mill.) were co-cultivated with Agrobacterium tumefaciens GV 3101 harboring binary vector pBI101 containing kanamycin resistance gene (npt II) as selection marker. Murashige and Skoog (MS) inorganic salts with Gamborg's B5 vitamins supplemented with optimized concentrations of zeatin riboside and indole-acetic acid resulted in enhanced ...
DOI:
10.1007/s10535-010-0060-9