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You searched for: Journal Biomaterials Remove constraint Journal: Biomaterials Subject chondrogenesis Remove constraint Subject: chondrogenesis
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1. 3D-bioprinted BMSC-laden biomimetic multiphasic scaffolds for efficient repair of osteochondral defects in an osteoarthritic rat model

Author:
Yanzhi Liu; Liuqi Peng; Lingli Li; Cuishan Huang; Keda Shi; Xiangbo Meng; Pinpin Wang; Mingming Wu; Ling Li; Huijuan Cao; Kefeng Wu; Qingqiang Zeng; Haobo Pan; William Weijia Lu; Ling Qin; Changshun Ruan; Xinluan Wang
Source:
Biomaterials 2021 v.279 pp. 121216
ISSN:
0142-9612
Subject:
animal models; biocompatible materials; biomimetics; cartilage; chondrogenesis; collagen; diclofenac; extracellular matrix; gait; hyaluronic acid; inflammation; mesenchymal stromal cells; osteoarthritis
Abstract:
... Osteochondral defect repair in osteoarthritis (OA) remains an unsolved clinical problem due to the lack of enough seed cells in the defect and chronic inflammation in the joint. To address this clinical need, we designed a bone marrow-derived mesenchymal stem cell (BMSC)-laden 3D-bioprinted multilayer scaffold with methacrylated hyaluronic acid (MeHA)/polycaprolactone incorporating kartogenin and ...
DOI:
10.1016/j.biomaterials.2021.121216

2. Biomaterial-mediated presentation of wnt5a mimetic ligands enhances chondrogenesis and metabolism of stem cells by activating non-canonical Wnt signaling

Author:
Yingrui Deng; Xiaoting Zhang; Rui Li; Zhuo Li; Boguang Yang; Peng Shi; Honglu Zhang; Chunming Wang; Chunyi Wen; Gang Li; Liming Bian
Source:
Biomaterials 2022 v.281 pp. 121316
ISSN:
0142-9612
Subject:
biocompatible materials; biomarkers; biosynthesis; cartilage; chondrogenesis; encapsulation; gene expression; glucose; humans; hyaluronic acid; hydrogels; hypertrophy; ligands; medicine; mitochondria; musculoskeletal system; peptides; tissue repair
Abstract:
... The presentation of development-relevant bioactive cues by biomaterial scaffolds is essential to the guided differentiation of seeded human mesenchymal stem cells (hMSCs) and subsequent tissue regeneration. Wnt5a is a critical non-canonical Wnt signaling ligand and plays a key role in the development of musculoskeletal tissues including cartilage. Herein we investigate the efficacy of biofunctiona ...
DOI:
10.1016/j.biomaterials.2021.121316

3. CRISPR activation of long non-coding RNA DANCR promotes bone regeneration

Author:
Nuong Thi Kieu Nguyen; Yu-Han Chang; Vu Anh Truong; Mu-Nung Hsu; Nam Ngoc Pham; Chin-Wei Chang; Yi-Hsiu Wu; Yi-Hao Chang; Hung Li; Yu-Chen Hu
Source:
Biomaterials 2021 v.275 pp. 120965
ISSN:
0142-9612
Subject:
Staphylococcus aureus; Streptococcus pyogenes; biocompatible materials; cartilage; chondrogenesis; non-coding RNA; rats; skull; therapeutics
Abstract:
... Healing of large calvarial bone defects in adults adopts intramembranous pathway and is difficult. Implantation of adipose-derived stem cells (ASC) that differentiate towards chondrogenic lineage can switch the bone repair pathway and improve calvarial bone healing. Long non-coding RNA DANCR was recently uncovered to promote chondrogenesis, but its roles in rat ASC (rASC) chondrogenesis and bone h ...
DOI:
10.1016/j.biomaterials.2021.120965

4. Chitosan hydrogel/3D-printed poly(ε‐caprolactone) hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment

Author:
Pinxue Li; Liwei Fu; Zhiyao Liao; Yu Peng; Chao Ning; Cangjian Gao; Daxu Zhang; Xiang Sui; Yunfeng Lin; Shuyun Liu; Chunxiang Hao; Quanyi Guo
Source:
Biomaterials 2021 v.278 pp. 121131
ISSN:
0142-9612
Subject:
DNA; biocompatible materials; cartilage; cell proliferation; chitosan; chondrogenesis; therapeutics
Abstract:
... Articular cartilage (AC) injury repair has always been a difficult problem for clinicians and researchers. Recently, a promising therapy based on mesenchymal stem cells (MSCs) has been developed for the regeneration of cartilage defects. As endogenous articular stem cells, synovial MSCs (SMSCs) possess strong chondrogenic differentiation ability and articular specificity. In this study, a cartilag ...
DOI:
10.1016/j.biomaterials.2021.121131

5. Chondrogenesis of adipose stem cells in a porous PLGA scaffold impregnated with plasmid DNA containing SOX trio (SOX-5,-6 and -9) genes

Author:
Gun-Il Im; Hye-Joung Kim; Jin H. Lee
Source:
Biomaterials 2011 v.32 no.19 pp. 4385-4392
ISSN:
0142-9612
Subject:
cartilage; chondrogenesis; genes; in vivo studies; leaching; plasmids; protein synthesis; proteoglycans; rabbits; stem cells
Abstract:
... We developed a chondrogenic scaffold system in which plasmid DNA (pDNA) containing SOX trio (SOX-5, -6, and -9) genes was incorporated into a PLGA scaffold and slowly released to transfect adipose stem cells (ASCs) seeded in the scaffold. The purpose of this study was to test the in vitro and in vivo efficacy of the system to induce chondrogenic differentiation of ASCs. The pDNA/PEI-PEG complex-in ...
DOI:
10.1016/j.biomaterials.2011.02.054

6. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds

Author:
Tali Re’em; Yael Kaminer-Israeli; Emil Ruvinov; Smadar Cohen
Source:
Biomaterials 2012 v.33 no.3 pp. 751-761
ISSN:
0142-9612
Subject:
binding capacity; bioactive properties; cartilage; cell differentiation; chondrocytes; chondrogenesis; collagen; humans; mice; signal transduction; stem cells; transforming growth factor beta 1
Abstract:
... Herein we describe a bio-inspired, affinity binding alginate-sulfate scaffold, designed for the presentation and sustained release of transforming growth factor beta 1 (TGF-β1), and examine its effects on the chondrogenesis of human mesenchymal stem cells (hMSCs). When attached to matrix via affinity interactions with alginate sulfate, TGF-β1 loading was significantly greater and its initial relea ...
DOI:
10.1016/j.biomaterials.2011.10.007

7. Chondrogenesis of human mesenchymal stem cells in fibrin constructs evaluated in vitro and in nude mouse and rabbit defects models

Author:
Ji S. Park; Han N. Yang; Dae G. Woo; Su Y. Jeon; Keun-H. Park
Source:
Biomaterials 2011 v.32 no.6 pp. 1495-1507
ISSN:
0142-9612
Subject:
Western blotting; animal models; cartilage; cell differentiation; chondrogenesis; denaturation; extracellular matrix; fibrin; genes; humans; hydrocolloids; immunohistochemistry; in vitro culture; in vivo studies; mice; protein synthesis; quantitative polymerase chain reaction; rabbits; reverse transcriptase polymerase chain reaction; stem cells; transforming growth factor beta 3
Abstract:
... In this study, hMSCs encapsulated in a fibrin hydrogel containing heparinized NPs loaded with TGF-β3 (100 ng/ml), or TGF-β3 (100 ng/ml) alone, were subjected to growth factor release and denaturation tests at one, two and four weeks in in vitro culture systems. Additionally, stem cell differentiation was assessed via RT-PCR, real-time quantitative PCR (qPCR), histology, and immunohistochemical ass ...
DOI:
10.1016/j.biomaterials.2010.11.003

8. Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles

Author:
Ji Sun Park; Han Na Yang; Dae Gyun Woo; Su Yeon Jeon; Hyun-Jin Do; Hye-Young Lim; Jae-Hwan Kim; Keun-Hong Park
Source:
Biomaterials 2011 v.32 no.14 pp. 3679-3688
ISSN:
0142-9612
Subject:
cell differentiation; chondrogenesis; ethyleneimine; fluorescence; green fluorescent protein; humans; in vitro culture; nanoparticles; polymers; reporter genes; stem cells; therapeutics; transfection
Abstract:
... Target gene transfection for desired cell differentiation has recently become a major issue in stem cell therapy. For the safe and stable delivery of genes into human mesenchymal stem cells (hMSCs), we employed a non-viral gene carrier system such as polycataionic polymer, poly(ethyleneimine) (PEI), polyplexed with a combination of SOX5, 6, and 9 fused to green fluorescence protein (GFP), yellow f ...
DOI:
10.1016/j.biomaterials.2011.01.063

9. Chondrogenesis of mesenchymal stem cells and dedifferentiated chondrocytes by transfection with SOX Trio genes

Author:
Han Na Yang; Ji Sun Park; Dae Gyun Woo; Su Yeon Jeon; Hyun-Jin Do; Hye-Young Lim; Seung Who Kim; Jae-Hwan Kim; Keun-Hong Park
Source:
Biomaterials 2011 v.32 no.30 pp. 7695-7704
ISSN:
0142-9612
Subject:
cartilage; chondrocytes; chondrogenesis; encapsulation; fibrin; genes; hydrocolloids; immunohistochemistry; mice; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; stem cells; transfection
Abstract:
... In this study, bone marrow-derived mesenchymal stem cells (MSCs), adipose-derived mesenchymal stem cells (ASCs) and dedifferentiated chondrocytes were transfected with SOX5, 6, and 9 genes (SOX Trio) and grown under pellet culture conditions (encapsulated in a fibrin hydrogel) to evaluate the chondrogenic potential in vitro and in vivo. RT-PCR, real-time quantitative PCR (qPCR), histology, and imm ...
DOI:
10.1016/j.biomaterials.2011.06.059

10. Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends

Author:
Nandana Bhardwaj; Subhas C. Kundu
Source:
Biomaterials 2012 v.33 no.10 pp. 2848-2857
ISSN:
0142-9612
Subject:
Antheraea mylitta; Bombyx mori; adults; bone marrow; cartilage; chitosan; chondrogenesis; collagen; confocal microscopy; cultured cells; electrolytes; genes; glycosaminoglycans; immunohistochemistry; mulberries; phenotype; quantitative polymerase chain reaction; rats; scanning electron microscopy; silk; silkworms; stem cells
Abstract:
... Adult bone marrow derived mesenchymal stem cells are undifferentiated, multipotential cells and have the potential to differentiate into multiple lineages like bone, cartilage or fat. In this study, polyelectrolyte complex silk fibroin/chitosan blended porous scaffolds were fabricated and examined for its ability to support in vitro chondrogenesis of mesenchymal stem cells. Silk fibroin matrices p ...
DOI:
10.1016/j.biomaterials.2011.12.028

11. Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3

Author:
Ji Sun Park; Myung-Sun Shim; Sung Han Shim; Han Na Yang; Su Yeon Jeon; Dae Gyun Woo; Dong Ryul Lee; Tae Ki Yoon; Keun-Hong Park
Source:
Biomaterials 2011 v.32 no.32 pp. 8139-8149
ISSN:
0142-9612
Subject:
adipose tissue; amniotic fluid; bone marrow; cartilage; cell differentiation; chondrocytes; chondrogenesis; fibrin; gels; genes; hydrocolloids; immunohistochemistry; mice; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; stem cells; transforming growth factor beta 3
Abstract:
... In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-β3 and then evaluated for their capacity for differentiation in vitro and in vivo. For determination of stem cell differentiation, RT-PCR, real time quantitative PCR (qPCR), histology, and immunohistochemical assays were used for analysis of chon ...
DOI:
10.1016/j.biomaterials.2011.07.043

12. Co-delivery of Cbfa-1-targeting siRNA and SOX9 protein using PLGA nanoparticles to induce chondrogenesis of human mesenchymal stem cells

Author:
Su Yeon Jeon; Ji Sun Park; Han Na Yang; Hye Jin Lim; Se Won Yi; Hansoo Park; Keun-Hong Park
Source:
Biomaterials 2014 v.35 no.28 pp. 8236-8248
ISSN:
0142-9612
Subject:
bioactive properties; bone formation; cell differentiation; chondrocytes; chondrogenesis; circular dichroism spectroscopy; extracellular matrix; humans; injection site; messenger RNA; mice; nanoparticles; protein content; small interfering RNA; staining; stem cells; transcription factors
Abstract:
... During stem cell differentiation, various cellular responses occur that are mediated by transcription factors and proteins. This study evaluated the abilities of SOX9, a crucial protein during the early stage of chondrogenesis, and siRNA targeting Cbfa-1, a transcription factor that promotes osteogenesis, to stimulate chondrogenesis. Non-toxic poly-(d,l-lactide-co-glycolide) (PLGA) nanoparticles ( ...
DOI:
10.1016/j.biomaterials.2014.05.092

13. Co-delivery of SOX9 genes and anti-Cbfa-1 siRNA coated onto PLGA nanoparticles for chondrogenesis of human MSCs

Author:
Su Yeon Jeon; Ji Sun Park; Han Na Yang; Dae Gyun Woo; Keun-Hong Park
Source:
Biomaterials 2012 v.33 no.17 pp. 4413-4423
ISSN:
0142-9612
Subject:
Western blotting; cell differentiation; chondrogenesis; ethyleneimine; fluorescent antibody technique; gels; gene expression; genes; green fluorescent protein; humans; image analysis; immunohistochemistry; light scattering; nanoparticles; plasmids; polymers; reverse transcriptase polymerase chain reaction; scanning electron microscopes; scanning electron microscopy; small interfering RNA; stem cells; transfection
Abstract:
... Some genes expressed in stem cells interrupt and/or enhance differentiation. Therefore, the aim of this study was to inhibit the expression of unnecessary genes and enhance the expression of specific genes involved in stem cell differentiation by using small interfering RNA (siRNA) and plasmid DNA (pDNA) incorporated into cationic polymers as co-delivery factors. To achieve co-delivery of siRNA an ...
DOI:
10.1016/j.biomaterials.2012.02.051

14. Collagen microencapsulation recapitulates mesenchymal condensation and potentiates chondrogenesis of human mesenchymal stem cells – A matrix-driven in vitro model of early skeletogenesis

Author:
Yuk Yin Li; Kwok Lim Lam; Abigail Dee Chen; Wei Zhang; Barbara Pui Chan
Source:
Biomaterials 2019 v.213 pp. 119210
ISSN:
0142-9612
Subject:
agglutinins; bone formation; cartilage; chondrogenesis; collagen; fibronectins; gene expression regulation; humans; integrins; mesenchymal stromal cells; microencapsulation; models; nanofibers; peanuts; tissue engineering; transcription factors
Abstract:
... Mesenchymal condensation is a critical transitional stage that precedes cartilage or bone formation. A microencapsulation technique was previously established to entrap mesenchymal stem cells (MSC) in nanofibrous collagen meshwork. We hypothesize that collagen microencapsulation of MSCs mimics the mesenchymal cell condensation process. Specifically, human MSCs at different concentrations were micr ...
DOI:
10.1016/j.biomaterials.2019.05.021

15. Controlling stem cell-mediated bone regeneration through tailored mechanical properties of collagen scaffolds

Author:
Hongli Sun; Feng Zhu; Qingang Hu; Paul H. Krebsbach
Source:
Biomaterials 2014 v.35 no.4 pp. 1176-1184
ISSN:
0142-9612
Subject:
bone formation; chondrogenesis; collagen; crosslinking; extracellular matrix; gelatin; histology; micro-computed tomography; microstructure; models; stem cells; strength (mechanics)
Abstract:
... Mechanical properties of the extracellular matrix (ECM) play an essential role in cell fate determination. To study the role of mechanical properties of ECM in stem cell-mediated bone regeneration, we used a 3D in vivo ossicle model that recapitulates endochondral bone formation. Three-dimensional gelatin scaffolds with distinct stiffness were developed using 1-Ethyl-3-[3-dimethylaminopropyl] carb ...
DOI:
10.1016/j.biomaterials.2013.10.054
PubMed:
24211076
PubMed Central:
PMC3926697

16. Cross-talk between TGF-beta/SMAD and integrin signaling pathways in regulating hypertrophy of mesenchymal stem cell chondrogenesis under deferral dynamic compression

Author:
Tianting Zhang; Feng Wen; Yingnan Wu; Graham Seow Hng Goh; Zigang Ge; Lay Poh Tan; James Hoi Po Hui; Zheng Yang
Source:
Biomaterials 2015 v.38 pp. 72-85
ISSN:
0142-9612
Subject:
CD29 antigen; cartilage; chondrogenesis; cytoskeleton; humans; hypertrophy; mechanotransduction; mitogen-activated protein kinase; stem cells
Abstract:
... The molecular mechanisms of mechanotransduction in regulating mesenchymal stem cell (MSC) chondrogenesis are not fully understood and represent an area of growing investigation. In this study, human MSC was subjected to chondrogenic differentiation in chitosan-coated poly l-lactide-co-ɛ-caprolactone scaffolds under free swelling or deferral dynamic compression conditions. The effect of deferral dy ...
DOI:
10.1016/j.biomaterials.2014.10.010

17. Cultured cell-derived extracellular matrix scaffolds for tissue engineering

Author:
Hongxu Lu; Takashi Hoshiba; Naoki Kawazoe; Ichie Koda; Minghui Song; Guoping Chen
Source:
Biomaterials 2011 v.32 no.36 pp. 9658-9666
ISSN:
0142-9612
Subject:
cartilage; cell adhesion; cell proliferation; chondrogenesis; extracellular matrix; fibroblasts; immunohistochemistry; stem cells; tissue engineering; tissue repair
Abstract:
... Cell-derived extracellular matrix (ECM) scaffolds have received considerable interest for tissue engineering applications. In this study, ECM scaffolds derived from mesenchymal stem cell (MSC), chondrocyte, and fibroblast were prepared by culturing cells in a selectively removable poly(lactic-co-glycolic acid) (PLGA) template. These three types of ECM scaffolds were used for in vitro cultures of M ...
DOI:
10.1016/j.biomaterials.2011.08.091

18. Decellularized adipose tissue microcarriers as a dynamic culture platform for human adipose-derived stem/stromal cell expansion

Author:
Claire Yu; Anna Kornmuller; Cody Brown; Todd Hoare; Lauren E. Flynn
Source:
Biomaterials 2017 v.120 pp. 66-80
ISSN:
0142-9612
Subject:
adipose tissue; bone formation; cell adhesion; cell differentiation; chondrogenesis; crosslinking; electrospraying; gene expression regulation; genes; humans; polystyrenes; protein synthesis; receptors; staining; stromal cells; tissue culture
Abstract:
... With the goal of designing a clinically-relevant expansion strategy for human adipose-derived stem/stromal cells (ASCs), methods were developed to synthesize porous microcarriers derived purely from human decellularized adipose tissue (DAT). An electrospraying approach was applied to generate spherical DAT microcarriers with an average diameter of 428 ± 41 μm, which were soft, compliant, and stabl ...
DOI:
10.1016/j.biomaterials.2016.12.017

19. Development of chimeric forms of the matrix metalloproteinase 2 collagen binding domain as artificial membrane binding proteins for targeting stem cells to cartilage lesions in osteoarthritic joints

Author:
Anais Dabbadie; Anna Salerno; Adam Perriman; Lu-Yun Lian; Anthony P. Hollander
Source:
Biomaterials 2022 v.285 pp. 121547
ISSN:
0142-9612
Subject:
amino acids; biocompatible materials; cartilage; chondrogenesis; collagen; gelatin; gelatinase A; ligands; solubility; stem cells
Abstract:
... Targeting stem cells to cartilage lesions has the potential to enhance engraftment and chondrogenesis. Denatured type II collagen fibrils (gelatin) are exposed in lesions at the surface of osteoarthritic articular cartilage and are therefore ideal target sites. We have designed and investigated chimeric mutants of the three modules of the MMP-2 collagen binding domain (CBD) as potential ligands fo ...
DOI:
10.1016/j.biomaterials.2022.121547

20. Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo

Author:
Xiaoqing Hu; Jingxian Zhu; Xiyu Li; Xin Zhang; Qingyang Meng; Lan Yuan; Jiying Zhang; Xin Fu; Xiaoning Duan; Haifeng Chen; Yingfang Ao
Source:
Biomaterials 2015 v.52 pp. 441-451
ISSN:
0142-9612
Subject:
X-ray diffraction; apatite; biocompatibility; bone marrow; cartilage; chondrogenesis; confocal microscopy; crystals; dextran; fluorescence; green fluorescent protein; hydrophilicity; image analysis; immunohistochemistry; monitoring; nanoparticles; reverse transcriptase polymerase chain reaction; stem cells; tissue engineering; transmission electron microscopy
Abstract:
... Upconversion fluorescent nanoparticles are becoming more widely used as imaging contrast agents, owing to their high resolution and penetration depth, and avoidance of tissue auto-fluorescence and photodamage to cells. Here, we synthesized upconversion fluorescent crystals from rare-earth Yb³⁺ and Ho³⁺ co-doped fluorapatite (FA:Yb³⁺/Ho³⁺) suitable for long-term tracking and monitoring cartilage de ...
DOI:
10.1016/j.biomaterials.2015.02.050

21. Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering

Author:
Heesun Hong; Ye Been Seo; Do Yeon Kim; Ji Seung Lee; Young Jin Lee; Hanna Lee; Olatunji Ajiteru; Md Tipu Sultan; Ok Joo Lee; Soon Hee Kim; Chan Hum Park
Source:
Biomaterials 2020 v.232 pp. 119679
ISSN:
0142-9612
Subject:
biocompatibility; biocompatible materials; biodegradability; biopolymers; cartilage; chondrogenesis; encapsulation; epithelium; fibroins; hydrogels; medicine; printers; rabbits; silk; three-dimensional printing; viability
Abstract:
... Three-dimensional printing with Digital Lighting Processing (DLP) printer has come into the new wave in the tissue engineering for regenerative medicine. Especially for the clinical application, it needs to develop of bio-ink with biocompatibility, biodegradability and printability. Therefore, we demonstrated that Silk fibroin as a natural polymer fabricated with glycidyl-methacrylate (Silk-GMA) f ...
DOI:
10.1016/j.biomaterials.2019.119679

22. Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering

Author:
Alexandra McMillan; Minh Khanh Nguyen; Tomas Gonzalez-Fernandez; Peilin Ge; Xiaohua Yu; William L. Murphy; Daniel J. Kelly; Eben Alsberg
Source:
Biomaterials 2018 v.161 pp. 240-255
ISSN:
0142-9612
Subject:
DNA; biomimetics; bone formation; bone morphogenetic proteins; bones; cartilage; cell aggregates; chondrogenesis; culture media; gene therapy; gene transfer; glycosaminoglycans; humans; hydroxyapatite; in vitro culture; microparticles; phenotype; stem cells; swine; tissue engineering; transforming growth factor beta 1; transgenes
Abstract:
... High-density mesenchymal stem cell (MSC) aggregates can be guided to form bone-like tissue via endochondral ossification in vitro when culture media is supplemented with proteins, such as growth factors (GFs), to first guide the formation of a cartilage template, followed by culture with hypertrophic factors. Recent reports have recapitulated these results through the controlled spatiotemporal del ...
DOI:
10.1016/j.biomaterials.2018.01.006
PubMed:
29421560
PubMed Central:
PMC5826638

23. Effects of bound versus soluble pentosan polysulphate in PEG/HA-based hydrogels tailored for intervertebral disc regeneration

Author:
Jessica E. Frith; Donna J. Menzies; Andrew R. Cameron; P. Ghosh; Darryl L. Whitehead; S. Gronthos; Andrew C.W. Zannettino; Justin J. Cooper-White
Source:
Biomaterials 2014 v.35 no.4 pp. 1150-1162
ISSN:
0142-9612
Subject:
cell proliferation; chemical bonding; chondrogenesis; coatings; collagen; culture media; gelation; histology; hydrogels; intervertebral disks; polyethylene glycol; polysaccharides; stem cells; therapeutics; transforming growth factor beta; viability
Abstract:
... Previous reports in the literature investigating chondrogenesis in mesenchymal progenitor cell (MPC) cultures have confirmed the chondro-inductive potential of pentosan polysulphate (PPS), a highly sulphated semi-synthetic polysaccharide, when added as a soluble component to culture media under standard aggregate-assay conditions or to poly(ethylene glycol)/hyaluronic acid (PEG/HA)-based hydrogels ...
DOI:
10.1016/j.biomaterials.2013.10.056

24. Effects of cell–cell contact and oxygen tension on chondrogenic differentiation of stem cells

Author:
Bin Cao; Zhenhua Li; Rong Peng; Jiandong Ding
Source:
Biomaterials 2015 v.64 pp. 21-32
ISSN:
0142-9612
Subject:
autocrine signaling; cell culture; chondrogenesis; collagen; ethylene glycol; gene expression; geometry; hydrocolloids; hypoxia; hypoxia-inducible factor 1; messenger RNA; normoxia; oxygen; polyethylene glycol; statistics; stem cells
Abstract:
... While cell condensation has been thought to enhance chondrogenesis, no direct evidence so far confirms that cell–cell contact itself increases chondrogenic differentiation of stem cells, since the change of cell–cell contact is usually coupled with those of other cell geometry cues and soluble factors in cell culture. The present study semi-quantitatively examined the effect of cell–cell contact i ...
DOI:
10.1016/j.biomaterials.2015.06.018

25. Effects of co-culturing BMSCs and auricular chondrocytes on the elastic modulus and hypertrophy of tissue engineered cartilage

Author:
Ning Kang; Xia Liu; Yue Guan; Jian Wang; Fuxing Gong; Xun Yang; Li Yan; Qian Wang; Xin Fu; Yilin Cao; Ran Xiao
Source:
Biomaterials 2012 v.33 no.18 pp. 4535-4544
ISSN:
0142-9612
Subject:
biomechanics; bone formation; cartilage; chondrocytes; chondrogenesis; coculture; gene expression; genes; hypertrophy; modulus of elasticity; quantitative analysis; stem cells
Abstract:
... Co-culture of BMSCs and chondrocytes is considered as a promising strategy to generate tissue engineered cartilage as chondrocytes induce the chondrogenesis of BMSCs and inhibit the hypertrophy of engineered cartilage. Because the tissue specific stem/progenitor cells have been isolated from mature tissues including auricular cartilage, we hypothesized that adding stem cells to auricular chondrocy ...
DOI:
10.1016/j.biomaterials.2012.03.019

26. Electrospun scaffolds for multiple tissues regeneration in vivo through topography dependent induction of lineage specific differentiation

Author:
Zi Yin; Xiao Chen; Hai-xin Song; Jia-jie Hu; Qiao-mei Tang; Ting Zhu; Wei-liang Shen; Jia-lin Chen; Huanhuan Liu; Boon Chin Heng; Hong-Wei Ouyang
Source:
Biomaterials 2015 v.44 pp. 173-185
ISSN:
0142-9612
Subject:
Achilles tendon; X-radiation; alkaline phosphatase; bone formation; bones; chondrogenesis; collagen; cytoskeleton; genetic markers; image analysis; immunohistochemistry; models; osteocalcin; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; stem cells; tissue engineering; tissue repair; topography
Abstract:
... Physical topographic cues from various substrata have been shown to exert profound effects on the growth and differentiation of stem cells due to their niche-mimicking features. However, the biological function of different topographic materials utilized as bio-scaffolds in vivo have not been rigorously characterized. This study investigated the divergent differentiation pathways of mesenchymal st ...
DOI:
10.1016/j.biomaterials.2014.12.027

27. Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs

Author:
Anna D. Dikina; Hannah A. Strobel; Bradley P. Lai; Marsha W. Rolle; Eben Alsberg
Source:
Biomaterials 2015 v.52 pp. 452-462
ISSN:
0142-9612
Subject:
animal models; biomechanics; chondrogenesis; gelatin; geometry; humans; microparticles; rats; stem cells; tissue engineering; tissue transplantation
Abstract:
... There is a critical need to engineer a neotrachea because currently there are no long-term treatments for tracheal stenoses affecting large portions of the airway. In this work, a modular tracheal tissue replacement strategy was developed. High-cell density, scaffold-free human mesenchymal stem cell-derived cartilaginous rings and tubes were successfully generated through employment of custom desi ...
DOI:
10.1016/j.biomaterials.2015.01.073
PubMed:
25818451
PubMed Central:
PMC4379457

28. Engineering cell matrix interactions in assembled polyelectrolyte fiber hydrogels for mesenchymal stem cell chondrogenesis

Author:
Deepak Raghothaman; Meng Fatt Leong; Tze Chiun Lim; Jerry K.C. Toh; Andrew C.A. Wan; Zheng Yang; Eng Hin Lee
Source:
Biomaterials 2014 v.35 no.9 pp. 2607-2616
ISSN:
0142-9612
Subject:
CD29 antigen; antibodies; chondrogenesis; collagen; electrolytes; extracellular matrix; humans; hydrocolloids; ligands; stem cells
Abstract:
... Cell–cell and cell–matrix interactions are important events in directing stem cell chondrogenesis, which can be promoted in matrix microenvironments presenting appropriate ligands. In this study, interfacial polyelectrolyte complexation (IPC) based hydrogels were employed, wherein the unique formation of submicron size fibers facilitated spatial orientation of ligands within such hydrogels. The in ...
DOI:
10.1016/j.biomaterials.2013.12.008

29. Enhanced MSC chondrogenesis following delivery of TGF-β3 from alginate microspheres within hyaluronic acid hydrogels in vitro and in vivo

Author:
Liming Bian; David Y. Zhai; Elena Tous; Reena Rai; Robert L. Mauck; Jason A. Burdick
Source:
Biomaterials 2011 v.32 no.27 pp. 6425-6434
ISSN:
0142-9612
Subject:
calcification; cartilage; chondrogenesis; coatings; culture media; gels; humans; hyaluronic acid; hydrocolloids; mechanical properties; mice; phenotype; stem cells; transforming growth factor beta 3
Abstract:
... Mesenchymal stem cells (MSCs) are being recognized as a viable cell source for cartilage repair and members of the transforming growth factor-beta (TGF-β) superfamily are a key mediator of MSC chondrogenesis. While TGF-β mediated MSC chondrogenesis is well established in in vitro pellet or hydrogel cultures, clinical translation will require effective delivery of TGF-βs in vivo. Here, we investiga ...
DOI:
10.1016/j.biomaterials.2011.05.033
PubMed:
21652067
PubMed Central:
PMC3134110

30. Enhanced chondrogenesis in co-cultures with articular chondrocytes and mesenchymal stem cells

Author:
Ville V. Meretoja; Rebecca L. Dahlin; F. Kurtis Kasper; Antonios G. Mikos
Source:
Biomaterials 2012 v.33 no.27 pp. 6362-6369
ISSN:
0142-9612
Subject:
DNA; cartilage; cattle; chondrocytes; chondrogenesis; coculture; collagen; cultured cells; engineering; gene expression; models; reverse transcriptase polymerase chain reaction; stem cells
Abstract:
... In this work, articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) with 1:1 and 1:3 cell ratios were co-cultured in order to evaluate if a majority of primary ACs can be replaced with MSCs without detrimental effects on in vitro chondrogenesis. We further used a xenogeneic culture model to study if such co-cultures can result in redifferentiation of passaged ACs. Cells were cultured in p ...
DOI:
10.1016/j.biomaterials.2012.05.042
PubMed:
22695067
PubMed Central:
PMC3392514

31. Exosome-mediated delivery of kartogenin for chondrogenesis of synovial fluid-derived mesenchymal stem cells and cartilage regeneration

Author:
Xiao Xu; Yujie Liang; Xingfu Li; Kan Ouyang; Manyi Wang; Tong Cao; Wencui Li; Jianquan Liu; Jianyi Xiong; Biquan Li; Jiang Xia; Daping Wang; Li Duan
Source:
Biomaterials 2021 v.269 pp. 120539
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; chondrocytes; chondrogenesis; cytosol; exosomes; membrane proteins; models; osteoarthritis; peptides; rats; stem cells; therapeutics; water solubility
Abstract:
... Transplantation of synovial fluid-derived mesenchymal stem cells (SF-MSCs) is a viable therapy for cartilage degeneration of osteoarthritis (OA). But controlling chondrogenic differentiation of the transplanted SF-MSCs in the joints remains a challenge. Kartogenin (KGN) is a small molecule that has been discovered to induce differentiation of SF-MSCs to chondrocytes both in vitro and in vivo. The ...
DOI:
10.1016/j.biomaterials.2020.120539

32. FLASH: Fluorescently LAbelled Sensitive Hydrogel to monitor bioscaffolds degradation during neocartilage generation

Author:
Carmine Onofrillo; Serena Duchi; Sam Francis; Cathal D. O'Connell; Lilith M. Caballero Aguilar; Stephanie Doyle; Zhilian Yue; Gordon G. Wallace; Peter F. Choong; Claudia Di Bella
Source:
Biomaterials 2021 v.264 pp. 120383
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; chemical bonding; chondrogenesis; ex vivo studies; extracellular matrix; fluorescence; fluorescent dyes; gelatin; humans; hydrogels
Abstract:
... Regenerative therapies based on photocrosslinkable hydrogels and stem cells are of growing interest in the field of cartilage repair. Cell-mediated degradation is critical for the successful clinical translation of implanted hydrogels. However, characterising cell-mediated degradation, while simultaneously monitoring the deposition of a distinct new matrix, remains a major challenge.In this study ...
DOI:
10.1016/j.biomaterials.2020.120383

33. Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds

Author:
Christopher R. Rowland; Lina A. Colucci; Farshid Guilak
Source:
Biomaterials 2016 v.91 pp. 57-72
ISSN:
0142-9612
Subject:
cartilage; chondrogenesis; collagen; crosslinking; crystals; epitopes; extracellular matrix; freezing; growth factors; ice; mechanical properties; porosity; stem cells; temperature; tissue engineering
Abstract:
... The native extracellular matrix of cartilage contains entrapped growth factors as well as tissue-specific epitopes for cell-matrix interactions, which make it a potentially attractive biomaterial for cartilage tissue engineering. A limitation to this approach is that the native cartilage extracellular matrix possesses a pore size of only a few nanometers, which inhibits cellular infiltration. Effo ...
DOI:
10.1016/j.biomaterials.2016.03.012
PubMed:
26999455
PubMed Central:
PMC4822340

34. Fibrous hyaluronic acid hydrogels that direct MSC chondrogenesis through mechanical and adhesive cues

Author:
Iris L. Kim; Sudhir Khetan; Brendon M. Baker; Christopher S. Chen; Jason A. Burdick
Source:
Biomaterials 2013 v.34 no.22 pp. 5571-5580
ISSN:
0142-9612
Subject:
adhesion; cartilage; chondrogenesis; collagen; cytoskeleton; extracellular matrix; gene expression; humans; hyaluronic acid; hydrocolloids; mechanics; stem cells; tissue engineering
Abstract:
... Electrospinning has recently gained much interest due to its ability to form scaffolds that mimic the nanofibrous nature of the extracellular matrix, such as the size and depth-dependent alignment of collagen fibers within hyaline cartilage. While much progress has been made in developing bulk, isotropic hydrogels for tissue engineering and understanding how the microenvironment of such scaffolds ...
DOI:
10.1016/j.biomaterials.2013.04.004
PubMed:
23623322
PubMed Central:
PMC3652578

35. Highly effective rheumatoid arthritis therapy by peptide-promoted nanomodification of mesenchymal stem cells

Author:
Yao Lu; Zhan Li; Lihua Li; Jieli Chen; Xingyi Xu; Zefeng Lin; Tao Zhang; Ye Zhu; Changhai Ding; Chuanbin Mao
Source:
Biomaterials 2022 v.283 pp. 121474
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; catalase; cell movement; chondrogenesis; inflammation; intravenous injection; metformin; oxidation; peptides; rats; rheumatoid arthritis; stem cells; superoxide dismutase
Abstract:
... Traditional medication is not satisfied in rheumatoid arthritis (RA) therapy due to its long-term side effects and failure in cartilage repair. Nanomodification of mesenchymal stem cells (MSCs) holds promise for lifting such hurdles but delivering therapeutic nanomaterials (NPs) into MSCs remains challenging in this new strategy. Here, we show that CuS@MnO₂ NPs functionalized with a short phage-se ...
DOI:
10.1016/j.biomaterials.2022.121474

36. In-situ birth of MSCs multicellular spheroids in poly(l-glutamic acid)/chitosan scaffold for hyaline-like cartilage regeneration

Author:
Kunxi Zhang; Shifeng Yan; Guifei Li; Lei Cui; Jingbo Yin
Source:
Biomaterials 2015 v.71 pp. 24-34
ISSN:
0142-9612
Subject:
air drying; cartilage; cell adhesion; chitosan; chondrogenesis; freeze drying; glutamic acid; polyglutamic acid; stem cells; tissue engineering
Abstract:
... The success of mesenchymal stem cells (MSCs) based articular cartilage tissue engineering is limited by the presence of fibrous tissue in generated cartilage, which is associated with the current scaffold strategy that promotes cellular adhesion and spreading. Here we design a non-fouling scaffold based on amide bonded poly(l-glutamic acid) (PLGA) and chitosan (CS) to drive adipose stem cells (ASC ...
DOI:
10.1016/j.biomaterials.2015.08.037

37. Influence of hyaluronic acid modification on CD44 binding towards the design of hydrogel biomaterials

Author:
Mi Y. Kwon; Chao Wang; Jonathan H. Galarraga; Ellen Puré; Lin Han; Jason A. Burdick
Source:
Biomaterials 2019 pp. -
ISSN:
0142-9612
Subject:
adhesion; alcohols; atomic force microscopy; biocompatible materials; carboxylic acids; chondrogenesis; crosslinking; disaccharides; encapsulation; glucuronic acid; hyaluronic acid; hydrogels; hydrophobicity; mesenchymal stromal cells; porous media; receptors
Abstract:
... Hyaluronic acid (HA) is a linear polysaccharide of d-glucuronic acid and N-acetyl-d-glucosamine that is native to many tissues and interacts with cells via cell-surface receptors (e.g., CD44). HA has been extensively explored as a chemically-modified macromer for crosslinking into biomaterials, such as hydrogels and macroporous scaffolds. However, the influence of the extent and type of HA modific ...
DOI:
10.1016/j.biomaterials.2019.119451
PubMed:
31480001
PubMed Central:
PMC6746338

38. Influence of stepwise chondrogenesis-mimicking 3D extracellular matrix on chondrogenic differentiation of mesenchymal stem cells

Author:
Rong Cai; Tomoko Nakamoto; Naoki Kawazoe; Guoping Chen
Source:
Biomaterials 2015 v.52 pp. 199-207
ISSN:
0142-9612
Subject:
chondrogenesis; collagen; extracellular matrix; immunohistochemistry; models; stem cells; tissue engineering; tissues
Abstract:
... Extracellular matrix (ECM) has drawn a broad attention for preparation of tissue engineering scaffolds and stem cell study. ECM scaffolds stepwise mimicking development of tissues can provide useful models to investigate the interactions between stem cells and ECM during the process of tissue development. In this study, 3D stepwise chondrogenesis-mimicking ECM scaffolds were prepared from mesenchy ...
DOI:
10.1016/j.biomaterials.2015.02.033

39. Injectable stem cell-laden supramolecular hydrogels enhance in situ osteochondral regeneration via the sustained co-delivery of hydrophilic and hydrophobic chondrogenic molecules

Author:
Jianbin Xu; Qian Feng; Sien Lin; Weihao Yuan; Rui Li; Jinming Li; Kongchang Wei; Xiaoyu Chen; Kunyu Zhang; Yanhua Yang; Tianyi Wu; Bin Wang; Meiling Zhu; Rui Guo; Gang Li; Liming Bian
Source:
Biomaterials 2019 v.210 pp. 51-61
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; chondrogenesis; crosslinking; drugs; encapsulation; gelatin; humans; hydrogels; hydrophilicity; hydrophobicity; mesenchymal stromal cells; models; rats; stem cells; therapeutics; transforming growth factor beta 1; viability
Abstract:
... Hydrogels have been widely used as the carrier material of therapeutic cell and drugs for articular cartilage repair. We previously demonstrated a unique host-guest macromer (HGM) approach to prepare mechanically resilient, self-healing and injectable supramolecular gelatin hydrogels free of chemical crosslinking. In this work, we show that compared with conventional hydrogels our supramolecular g ...
DOI:
10.1016/j.biomaterials.2019.04.031

40. In vitro chondrogenesis and in vivo repair of osteochondral defect with human induced pluripotent stem cells

Author:
Ji-Yun Ko; Kyung-Il Kim; Siyeon Park; Gun-Il Im
Source:
Biomaterials 2014 v.35 no.11 pp. 3571-3581
ISSN:
0142-9612
Subject:
alginates; cartilage; chondrogenesis; collagen; epigenetics; glycosaminoglycans; humans; hydrogels; hypertrophy; induced pluripotent stem cells; methylation; pellets; rats
Abstract:
... The purpose of this study was to investigate the chondrogenic features of human induced pluripotent stem cells (hiPSCs) and examine the differences in the chondrogenesis between hiPSCs and human bone marrow-derived MSCs (hBMMSCs). Embryoid bodies (EBs) were formed from undifferentiated hiPSCs. After EBs were dissociated into single cells, chondrogenic culture was performed in pellets and alginate ...
DOI:
10.1016/j.biomaterials.2014.01.009

41. Label-free separation of mesenchymal stem cell subpopulations with distinct differentiation potencies and paracrine effects

Author:
Lu Yin; Zheng Yang; Yingnan Wu; Vinitha Denslin; Chia Chen Yu; Ching Ann Tee; Chwee Teck Lim; Jongyoon Han; Eng Hin Lee
Source:
Biomaterials 2020 v.240 pp. 119881
ISSN:
0142-9612
Subject:
adipogenesis; biocompatible materials; bone formation; bone marrow; cell senescence; chondrogenesis; humans; mesenchymal stromal cells; remission; therapeutics; tissue repair
Abstract:
... Mesenchymal stem cells (MSCs) have the capability to differentiate into multiple cell lineages, and produce trophic factors to facilitate tissue repair and regeneration, and disease regression. However, the heterogeneity of MSCs, whether inherent or developed during culture expansion, has a significant impact on their therapeutic efficacy. Therefore, the ability to identify and select an efficacio ...
DOI:
10.1016/j.biomaterials.2020.119881

42. Local delivery of a carbohydrate analog for reducing arthritic inflammation and rebuilding cartilage

Author:
Chaekyu Kim; Ok Hee Jeon; Do Hun Kim; J. Jeremy Chae; Lucas Shores; Nicholas Bernstein; Rahul Bhattacharya; Jeannine M. Coburn; Kevin J. Yarema; Jennifer H. Elisseeff
Source:
Biomaterials 2016 v.83 pp. 93-101
ISSN:
0142-9612
Subject:
animal models; cartilage; chondrocytes; chondrogenesis; drugs; etiology; humans; inflammation; osteoarthritis; stem cells; therapeutics; tissue engineering
Abstract:
... Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degradation. Because OA has a multifactorial nature and complex interrelationship of the individual elements of a whole joint, there is a need for comprehensive therapeutic approaches for cartilage tissue engineering, which simultaneously address multiple aspects of disease etiology. In this work, we investiga ...
DOI:
10.1016/j.biomaterials.2015.12.029

43. Mechanically cartilage-mimicking poly(PCL-PTHF urethane)/collagen nanofibers induce chondrogenesis by blocking NF–kappa B signaling pathway

Author:
Tongmeng Jiang; Dan Kai; Sijia Liu; Xianyuan Huang; Shujun Heng; Jinmin Zhao; Benjamin Qi Yu Chan; Xian Jun Loh; Ye Zhu; Chuanbin Mao; Li Zheng
Source:
Biomaterials 2018 v.178 pp. 281-292
ISSN:
0142-9612
Subject:
calves; cartilage; chondrogenesis; collagen; inflammation; mechanical properties; messenger RNA; nanofibers; nucleotide sequences; signal transduction; stem cells; urethane
Abstract:
... Cartilage cannot self-repair and thus regeneration is a promising approach to its repair. Here we developed new electrospun nanofibers, made of poly (ε-caprolactone)/polytetrahydrofuran (PCL-PTHF urethane) and collagen I from calf skin (termed PC), to trigger the chondrogenic differentiation of mesenchymal stem cells (MSCs) and the cartilage regeneration in vivo. We found that the PC nanofibers ha ...
DOI:
10.1016/j.biomaterials.2018.06.023
PubMed:
29945065
PubMed Central:
PMC6301151

44. Mechano growth factor (MGF) and transforming growth factor (TGF)-β3 functionalized silk scaffolds enhance articular hyaline cartilage regeneration in rabbit model

Author:
Ziwei Luo; Li Jiang; Yan Xu; Haibin Li; Wei Xu; Shuangchi Wu; Yuanliang Wang; Zhenyu Tang; Yonggang Lv; Li Yang
Source:
Biomaterials 2015 v.52 pp. 463-475
ISSN:
0142-9612
Subject:
animal models; cartilage; chemotaxis; chondrogenesis; collagen; drugs; extracellular matrix; fibroins; histology; insulin-like growth factor I; methanol; osteoarthritis; rabbits; secretion; silk; staining; stem cells; tissue engineering; transforming growth factor beta 3
Abstract:
... Damaged cartilage has poor self-healing ability and usually progresses to scar or fibrocartilaginous tissue, and finally degenerates to osteoarthritis (OA). Here we demonstrated that one of alternative isoforms of IGF-1, mechano growth factor (MGF) acted synergistically with transforming growth factor β3 (TGF-β3) embedded in silk fibroin scaffolds to induce chemotactic homing and chondrogenic diff ...
DOI:
10.1016/j.biomaterials.2015.01.001

45. Mesenchymal cells condensation-inducible mesh scaffolds for cartilage tissue engineering

Author:
In Gul Kim; Jaehoon Ko; Hye Rim Lee; Sun Hee Do; Kwideok Park
Source:
Biomaterials 2016 v.85 pp. 18-29
ISSN:
0142-9612
Subject:
cadherins; cartilage; cell adhesion; cell aggregates; chondrogenesis; extracellular matrix; fibroblasts; fibronectins; gene expression regulation; genes; heparin; histology; humans; in vitro culture; lungs; models; non-specific protein-tyrosine kinase; protein content; rabbits; stem cells; tissue engineering; transforming growth factor beta 1; umbilical cord
Abstract:
... Mesenchymal cells condensation is crucial in chondrogenic development. However current tissue-engineered scaffolds for chondrogenesis pay little attention to this phenomenon. In this study, we fabricate poly(l-lactide-co-glycolide) (PLGA)/poly(l-lactide) (PLLA) microfiber scaffolds and coat them with human fibroblast-derived matrix (hFDM) that is a decellularized extracellular matrix (ECM) obtaine ...
DOI:
10.1016/j.biomaterials.2016.01.048

46. MicroRNA regulation associated chondrogenesis of mouse MSCs grown on polyhydroxyalkanoates

Author:
Chao Yan; Yang Wang; Xiao-Yan Shen; Gang Yang; Jia Jian; Hua-Su Wang; Guo-Qiang Chen; Qiong Wu
Source:
Biomaterials 2011 v.32 no.27 pp. 6435-6444
ISSN:
0142-9612
Subject:
cell differentiation; chondrocytes; chondrogenesis; collagen; extracellular matrix; gene expression regulation; genes; genetic markers; mice; microRNA; phenotype; polyhydroxyalkanoates; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; secretion; stem cells
Abstract:
... Microbial polyhydroxyalkanoates (PHA) including poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) were found to induce chondrogenesis of mesenchymal stem cells (MSCs) and preserve chondrocytic phenotype as well as support chondrocytes-specific extracellular matrix (ECM) secretion. In this study, mouse MSCs cultured on the PHBHHx films for 24 h showed up-regulated expression of chondrogenic ma ...
DOI:
10.1016/j.biomaterials.2011.05.031

47. Modulation of mesenchymal stem cell chondrogenesis in a tunable hyaluronic acid hydrogel microenvironment

Author:
Wei Seong Toh; Teck Chuan Lim; Motoichi Kurisawa; Myron Spector
Source:
Biomaterials 2012 v.33 no.15 pp. 3835-3845
ISSN:
0142-9612
Subject:
crosslinking; contracture; biosynthesis; phenotype; morphogenesis; biomimetics; hydrocolloids; goats; hydrogen peroxide; chondrogenesis; cartilage; collagen; stem cells; oxidants; biodegradability; tissue engineering; hyaluronic acid; peroxidase; gelation
Abstract:
... An injectable and biodegradable hydrogel system comprising hyaluronic acid-tyramine (HA-Tyr) conjugates can safely undergo covalent cross-linking in vivo by the addition of small amounts of peroxidase and hydrogen peroxide (H₂O₂), with the independent tuning of the gelation rate and degree of cross-linking. Such hydrogel networks with tunable mechanical and degradation properties may provide the a ...
DOI:
10.1016/j.biomaterials.2012.01.065

48. Molecular mechanism of hypoxia-induced chondrogenesis and its application in in vivo cartilage tissue engineering

Author:
Elise Duval; Catherine Baugé; Rina Andriamanalijaona; Hervé Bénateau; Sylvain Leclercq; Soizic Dutoit; Laurent Poulain; Philippe Galéra; Karim Boumédiene
Source:
Biomaterials 2012 v.33 no.26 pp. 6042-6051
ISSN:
0142-9612
Subject:
biotechnology; bone marrow; cartilage; chondrocytes; chondrogenesis; cultured cells; gene overexpression; growth factors; humans; hypoxia; medicine; mice; phenotype; stem cells; tissue analysis; tissue engineering; transcription factors
Abstract:
... Cartilage engineering is one of the most challenging issue in regenerative medicine, due to its limited self-ability to repair. Here, we assessed engineering of cartilage tissue starting from human bone marrow (hBM) stem cells under hypoxic environment and delineated the mechanism whereby chondrogenesis could be conducted without addition of exogenous growth factors. hBM stem cells were cultured i ...
DOI:
10.1016/j.biomaterials.2012.04.061

49. Multilineage differentiation of human-derived dermal fibroblasts transfected with genes coated on PLGA nanoparticles plus growth factors

Author:
Ji Sun Park; Han Na Yang; Dae Gyun Woo; Su Yeon Jeon; Keun-Hong Park
Source:
Biomaterials 2013 v.34 pp. 582-597
ISSN:
0142-9612
Subject:
Western blotting; adipogenesis; bone formation; chondrogenesis; confocal laser scanning microscopy; fibrin; fibroblasts; gels; genes; mice; nanoparticles; polymers; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; somatomedins; tissue repair; transforming growth factor beta 3
Abstract:
... Wounded tissues and cells may be treated with growth factors and specific genes for the purpose of tissue repair and regeneration. To deliver specific genes into tissues and cells, this study presents the use of fabricated poly (dl-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) complexed with the cationic polymer poly (ethleneimine) (PEI). Through complexation with PEI, several types of genes ...
DOI:
10.1016/j.biomaterials.2012.10.001

50. PLGA-based microcarriers induce mesenchymal stem cell chondrogenesis and stimulate cartilage repair in osteoarthritis

Author:
Marie Morille; Karine Toupet; Claudia N. Montero-Menei; Christian Jorgensen; Danièle Noël
Source:
Biomaterials 2016 v.88 pp. 60-69
ISSN:
0142-9612
Subject:
cartilage; chondrocytes; chondrogenesis; confocal laser scanning microscopy; histology; humans; mice; microparticles; osteoarthritis; stem cells; stromal cells
Abstract:
... In the present study, we aimed at evaluating the ability of novel PLGA-P188-PLGA-based microspheres to induce the differentiation of mesenchymal stem/stromal cells (MSC) into chondrocytes. To this aim, we tested microspheres releasing TGFβ3 (PAM-T) in vitro and in situ, in a pathological osteoarthritic (OA) environment. We first evaluated the chondrogenic differentiation of human MSCs seeded onto ...
DOI:
10.1016/j.biomaterials.2016.02.022