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You searched for: Journal FEMS microbiology letters Remove constraint Journal: FEMS microbiology letters Subject term Escherichia coli Remove constraint Subject term: Escherichia coli
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1. Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA

Author:
Christopher Keller; Manas Chattopadhyay; Herbert Tabor
Source:
FEMS microbiology letters 2019 v.366 no.10 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; genes; messenger RNA; mutants; polyamines; protein synthesis; ribosomes; transfer RNA; uridine
Abstract:
... The genes mnmE and mnmG are responsible for the modification of uridine 34, ‘the wobble position’ of many aminoacyl-tRNAs. Deletion of these genes affects the strength of the codon-anticodon interactions of the aminoacyl-tRNAs with the mRNAs and the ribosomes. However, deletion of these genes does not usually have a significant effect on the growth rate of the standard Escherichia coli strains. In ...
DOI:
10.1093/femsle/fnz110
PubMed:
31162608
PubMed Central:
PMC6700685

2. The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

Author:
Thomas Jacobsen; Chunyu Liao; Chase L Beisel
Source:
FEMS microbiology letters 2019 v.366 no.8 pp. -
ISSN:
1574-6968
Subject:
Acidaminococcus; DNA damage; Escherichia coli; consensus sequence; gene editing; mammals; nucleases; plasmids
Abstract:
... The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nuclease Acidaminococcus sp. Cas12a (AsCas12a, also known as AsCpf1) has become a popular alternative to Cas9 for genome editing and other applications. AsCas12a has been associated with a TTTV protospacer-adjacent motif (PAM) as part of target recognition. Using a cell-free transcription-translation (TXTL)-base ...
DOI:
10.1093/femsle/fnz085
PubMed:
31004485
PubMed Central:
PMC6604746

3. Addiction systems antagonize bacterial adaptive immunity

Author:
Lisa van Sluijs; Stineke van Houte; John van der Oost; Stan JJ Brouns; Angus Buckling; Edze R Westra
Source:
FEMS microbiology letters 2019 v.366 no.5 pp. -
ISSN:
1574-6968
Subject:
CRISPR-Cas systems; Escherichia coli; adaptive immunity; antibiotic resistance; antibiotics; genes; interspersed repetitive sequences; plasmids; toxicity; toxins
Abstract:
... CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spread of antibiotic resistance, will depend on the fitness costs of employing CRISPR ...
DOI:
10.1093/femsle/fnz047
PubMed:
30834930
PubMed Central:
PMC6478593

4. The BFK20 phage replication origin confers a phage-encoded resistance phenotype to the industrial strain Brevibacterium flavum

Author:
Gabriela Bukovska; Jana Ugorcakova; Nora Halgasova; Lucia Bocanova; Adela Tkacova
Source:
FEMS microbiology letters 2019 v.366 no.8 pp. -
ISSN:
1574-6968
Subject:
Brevibacterium; Corynebacterium glutamicum; Escherichia coli; Southern blotting; antibiotic resistance; bioinformatics; hosts; monitoring; phages; phenotype; plasmids; replication origin; resistance genes; tetracycline; viability
Abstract:
... The phage BFK20 replication origin was identified using bioinformatics tools and a fragment with the origin nucleotide sequence was cloned into the tetracycline resistance gene of Escherichia coli vector pBR328, to make the plasmid pBOS. After transformation into the host strain Brevibacterium flavum CCM 251, pBOS was able to replicate, showing that the cloned region may function as a replication ...
DOI:
10.1093/femsle/fnz090

5. Bio-surfactin stabilised silver nanoparticles exert inhibitory effect over New-Delhi metallo-beta-lactamases (NDMs) and the cells harbouring NDMs

Author:
Ganesh Kumar N; Gaurav Kumar; Sathi Mallick; Sudip Kumar Ghosh; Panchanan Pramanick; Anindya S Ghosh
Source:
FEMS microbiology letters 2019 v.366 no.10 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; antimicrobial properties; beta-lactamase; cell viability; cephalosporins; cystine; genes; hydrolysis; ions; nanosilver; silver
Abstract:
... Silver nanoparticles (AgNPs) are used as an antimicrobial agent since the ages. However, it is unknown whether AgNPs exert inhibitory effects over the bacterial cells carrying metallo-beta-lactamases (MBLs). Here, using bio-surfactin stabilised AgNPs having a size range from 5 to 25 nm we established its antimicrobial effects against NDMs harbouring cells. Antimicrobial effectiveness of AgNPs is a ...
DOI:
10.1093/femsle/fnz118

6. Biological activities associated with the volatile compound 2,5-bis(1-methylethyl)-pyrazine

Author:
Thierry K S Janssens; Olaf Tyc; Harrie Besselink; Wietse de Boer; Paolina Garbeva
Source:
FEMS microbiology letters 2019 v.366 no.3 pp. -
ISSN:
1574-6968
Subject:
Burkholderia; DNA damage; Escherichia coli; Paenibacillus; adverse outcome pathways; antimicrobial properties; aromatic compounds; cell walls; coculture; cytotoxicity; food industry; fumigants; luciferase; mammals; mechanism of action; neoplasms; peptidoglycans; pyrazines; toxicology; transcription (genetics); transcription factors; volatile organic compounds
Abstract:
... Pyrazines are 1,4-diazabenzene-based volatile organic compounds and known for their broad-spectrum antimicrobial activity. In the present study, we assessed the antimicrobial activity of 2,5-bis(1-methylethyl)-pyrazine, produced by Paenibacillus sp. AD87 during co-culture with Burkholderia sp. AD24. In addition, we were using transcriptional reporter assays in E. coli and mammalian cells to deciph ...
DOI:
10.1093/femsle/fnz023

7. Cas3-stimulated runaway replication of modified ColE1 plasmids in Escherichia coli is temperature dependent

Author:
Marin Radovčić; Anja Čulo; Ivana Ivančić-Baće
Source:
FEMS microbiology letters 2019 v.366 no.9 pp. -
ISSN:
1574-6968
Subject:
CRISPR-Cas systems; CRISPR-associated proteins; Escherichia coli; RNA; adaptive immunity; enzyme activity; gene editing; genotype; interspersed repetitive sequences; mutants; mutation; phages; plasmids; prokaryotic cells; temperature
Abstract:
... The clustered regularly interspersed short palindromic repeats (CRISPR)-Cas system constitutes an adaptive immunity system of prokaryotes against mobile genetic elements using a CRISPR RNA (crRNA)-mediated interference mechanism. In Type I CRISPR-Cas systems, crRNA guided by a Cascade complex recognises the matching target DNA and promotes an R-loop formation, RNA-DNA hybrid. The helicase-nuclease ...
DOI:
10.1093/femsle/fnz106

8. Chaperonin overproduction and metabolic erosion caused by mutation accumulation in Escherichia coli

Author:
José Aguilar-Rodríguez; Mario A Fares; Andreas Wagner
Source:
FEMS microbiology letters 2019 v.366 no.10 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; biochemical pathways; carbon; chaperonins; enzymes; genetic drift; genome; insects; microarray technology; microsymbionts; monitoring; mutation accumulation; phenotype
Abstract:
... Bacterial cells adapting to a constant environment tend to accumulate mutations in portions of their genome that are not maintained by selection. This process has been observed in bacteria evolving under strong genetic drift, and especially in bacterial endosymbionts of insects. Here, we study this process in hypermutable Escherichia coli populations evolved through 250 single-cell bottlenecks on ...
DOI:
10.1093/femsle/fnz121

9. Comparison of the expression of phospholipase D from Streptomyces halstedii in different hosts and its over-expression in Streptomyces lividans

Author:
Xinyi Tao; Ming Zhao; Ying Zhang; Min Liu; Qinghai Liu; Wei Wang; Feng-Qing Wang; Dongzhi Wei
Source:
FEMS microbiology letters 2019 v.366 no.5 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; Komagataella pastoris; Streptomyces halstedii; Streptomyces lividans; biocatalysts; enzyme activity; food industry; gene expression; genetically modified organisms; phosphatidylcholines; phospholipase D; proteins; toxicity
Abstract:
... Phospholipase D (PLD) proteins from Streptomyces species are useful biocatalysts for synthesizing phospholipid derivatives relevant for the pharmaceutical and food industry from low-cost phosphatidylcholine. The overexpression of PLD in a recombinant strain is necessary to achieve large-scale PLD production. In this study, we investigated the feasibility of expressing PLD from Streptomyces halsted ...
DOI:
10.1093/femsle/fnz051

10. Detection of fecal bacteria and antibiotic resistance genes in drinking water collected from three First Nations communities in Manitoba, Canada

Author:
Ruidong Mi; Rakesh Patidar; Annemieke Farenhorst; Zhangbin Cai; Shadi Sepehri; Ehsan Khafipour; Ayush Kumar
Source:
FEMS microbiology letters 2019 v.366 no.6 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; First Nations; antibiotic resistance genes; aquifers; chlorine; coliform bacteria; drinking water; fecal bacteria; lakes; microbiological quality; plate count; water treatment; well water; Manitoba
Abstract:
... This study analyzed the microbiological quality of drinking and source water from three First Nations communities in Manitoba, Canada that vary with respect to the source, storage and distribution of drinking water. Community A relies on an aquifer and Community B on a lake as source water to their water treatment plants. Community C does not have a water treatment plant and uses well water. Quant ...
DOI:
10.1093/femsle/fnz067
PubMed:
30980671
PubMed Central:
PMC6604745

11. Effects of low-intensity and low-frequency ultrasound combined with tobramycin on biofilms of extended-spectrum beta-lactamases (ESBLs) Escherichia coli

Author:
Yuru Hou; Min Yang; Hexun Jiang; Dairong Li; Yonghong Du
Source:
FEMS microbiology letters 2019 v.366 no.3 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; antibacterial properties; bacteria; beta-lactamase; biofilm; confocal laser scanning microscopy; multiple drug resistance; scanning electron microscopy; tobramycin; ultrasonics; viability
Abstract:
... Low-intensity and low-frequency ultrasound (LILFU) can enhance the bactericidal action of antibiotics against various sensitive bacterial species. The current study investigated the effects of LILFU combined with tobramycin on extended-spectrum beta-lactamases (ESBLs) Escherichia coli biofilms (a multi-drug resistant bacteria). The biofilms of ESBLs E. coli were established and treated with ultras ...
DOI:
10.1093/femsle/fnz026

12. Identification of novel protein domain for sialyloligosaccharide binding essential to Mycoplasma mobile gliding

Author:
Tasuku Hamaguchi; Masaru Kawakami; Hidemitsu Furukawa; Makoto Miyata
Source:
FEMS microbiology letters 2019 v.366 no.3 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; Mycoplasma mobile; adhesins; amino acid sequences; animal tissues; atomic force microscopy; bacteria; fetuins; fish; glycoconjugates; protein domains; sialic acids; viruses
Abstract:
... Sialic acids, terminal structures of sialylated glycoconjugates, are widely distributed in animal tissues and are often involved in intercellular recognitions, including some bacteria and viruses. Mycoplasma mobile, a fish pathogenic bacterium, binds to sialyloligosaccharide (SO) through adhesin Gli349 and glides on host cell surfaces. The amino acid sequence of Gli349 shows no similarity to known ...
DOI:
10.1093/femsle/fnz016
PubMed:
30668689
PubMed Central:
PMC6376172

13. Loop-mediated isothermal amplification assays for Enterococcussp., Escherichiacoli and Staphylococcusaureus in chicken

Author:
HyeSoon Song; YouChan Bae; HyukMan Kwon; YongKuk Kwon; SeongJoon Joh
Source:
FEMS microbiology letters 2019 v.366 no.5 pp. -
ISSN:
1574-6968
Subject:
Enterococcus avium; Enterococcus cecorum; Enterococcus durans; Enterococcus faecalis; Enterococcus faecium; Enterococcus gallinarum; Enterococcus hirae; Escherichia coli; Staphylococcus aureus; broiler chickens; diagnostic techniques; economic impact; financial economics; lameness; loop-mediated isothermal amplification; microorganisms; osteomyelitis; pathogenesis; pathogens
Abstract:
... Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chicken, and results in serious economic losses worldwide. Although the pathogenesis mechanism leading to lameness is not entirely understood, some strains of Enterococcussp., avian pathogenic Escherichia coli or Staphylococcus aureus have been long recognized as important causative pathogens. To prevent the ...
DOI:
10.1093/femsle/fnz042
PubMed:
30806654
PubMed Central:
PMC6483310

14. MgrB affects the acid stress response of Escherichia coli by modulating the expression of iraM

Author:
Jintian Xu; Ting Li; Yunrong Gao; Jiaoyu Deng; Jing Gu
Source:
FEMS microbiology letters 2019 v.366 no.11 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli K12; acid tolerance; developmental stages; gene expression regulation; genes; mutants; mutation; stress response; survival rate; transcriptomics
Abstract:
... Although MgrB is established to be a feedback inhibitor of the PhoP/Q system in Escherichia coli, the biological functions of MgrB remain largely unknown. To explore new functions of MgrB, a comparative transcriptome analysis was performed (E. coli K-12 W3110 ΔmgrB vs E. coli K-12 W3110). The results showed that many genes involved in acid stress are upregulated, suggesting that MgrB is related to ...
DOI:
10.1093/femsle/fnz123

15. Peptidyl isomerase PrsA is surface-associated on Streptococcus suis and offers cross-protection against serotype 9 strain

Author:
Xiaowu Jiang; Yunkai Yang; Jingjing Zhou; Hanze Liu; Xiayi Liao; Jie Luo; Xiaoliang Li; Weihuan Fang
Source:
FEMS microbiology letters 2019 v.366 no.2 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; Streptococcus suis; animal models; antibody formation; bacteria; cross immunity; genes; immunization; immunogenicity; isomerases; mice; microbial physiology; pathogenicity; proteins; serotypes; subunit vaccines
Abstract:
... PrsA, a peptidyl isomerase encoded by the prsA gene, plays pleiotropic roles in bacterial physiology and pathogenicity. This study was attempted to characterize the distribution of prsA in different serotypes of Streptococcus suis isolates and on the bacterial cells and to evaluate its immunogenicity in a murine model. PrsA is present in different S. suis types and surface-associated as tested in ...
DOI:
10.1093/femsle/fnz002

16. Prevalence of antibiotic-resistant Escherichia coli isolated from urban and agricultural streams in Canterbury, New Zealand

Author:
Sophie Van Hamelsveld; Muyiwa E Adewale; Brigitta Kurenbach; William Godsoe; Jon S Harding; Mitja N P Remus-Emsermann; Jack A Heinemann
Source:
FEMS microbiology letters 2019 v.366 no.8 pp. -
ISSN:
1574-6968
Subject:
Escherichia coli; agroecosystems; antibiotic resistance; antibiotics; freshwater; freshwater ecosystems; human health; ingestion; risk; rivers; sediments; streams; urban areas; virulent strains; New Zealand
Abstract:
... Baseline studies are needed to identify environmental reservoirs of non-pathogenic but associating microbiota or pathogenic bacteria that are resistant to antibiotics and to inform safe use of freshwater ecosystems in urban and agricultural settings. Mesophilic bacteria and Escherichia coli were quantified and isolated from water and sediments of two rivers, one in an urban and one in an agricultu ...
DOI:
10.1093/femsle/fnz104

17. Resveratrol controls Escherichia coli growth by inhibiting the AcrAB-TolC efflux pump

Author:
Dahyun Hwang; Young-Hee Lim
Source:
FEMS microbiology letters 2019 v.366 no.4 pp. -
ISSN:
1574-6968
Subject:
DNA microarrays; Escherichia coli; antibacterial properties; drugs; genes; grapes; growth retardation; iron absorption; mutants; phytoalexins; resveratrol; transporters
Abstract:
... Resveratrol (RSV), a phytoalexin found in grapes and other plants, is known to have antibacterial effects against Escherichia coli. In this study, we aimed to identify the target gene(s) for the antibacterial activity of RSV in E. coli. Using a DNA microarray, we found that exposure to RSV led to changes in the expression levels of iron metabolism genes, and those involved in drug response and res ...
DOI:
10.1093/femsle/fnz030

18. Two extremely divergent sequence forms of the genes that define Escherichia coli group 3 capsules suggest a very long history since their common ancestor

Author:
Yaoqin Hong; Monica M Cunneen; Peter R Reeves
Source:
FEMS microbiology letters 2019 v.366 no.8 pp. -
ISSN:
1574-6968
Subject:
ABC transporters; Escherichia coli; ancestry; multigene family; virulence
Abstract:
... Capsules are a critical virulence factor in many pathogenic Escherichia coli, of which groups 2 and 3 capsules are synthesised by the ABC transporter pathway. The well-studied forms are in group 2 and much of our knowledge of group 3 is inferred from our understanding of group 2. We analyse six group 3 gene clusters including representatives of K10, K11 and K96, and find unexpected diversity. Grou ...
DOI:
10.1093/femsle/fnz091

19. Unexpected connections between type VI-B CRISPR-Cas systems, bacterial natural competence, ubiquitin signaling network and DNA modification through a distinct family of membrane proteins

Author:
Kira S Makarova; Linyi Gao; Feng Zhang; Eugene V Koonin
Source:
FEMS microbiology letters 2019 v.366 no.8 pp. -
ISSN:
1574-6968
Subject:
Bacteroidetes; CRISPR-Cas systems; Escherichia coli; bacteria; loci; membrane proteins; messenger RNA; operon; prediction; single-stranded DNA; ubiquitin
Abstract:
... In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We show that Csx27 belongs to an expansive family of proteins that contain four predic ...
DOI:
10.1093/femsle/fnz088
PubMed:
31089700
PubMed Central:
PMC6700684

20. A color-based competition assay for studying bacterial stress responses in Micrococcus luteus

Author:
Spencer Havis; Jonathan Rangel; Sujina Mali; Abiodun Bodunrin; Zina Housammy; Rene Zimmerer; Jesse Murphy; William R Widger; Steven J Bark
Source:
FEMS microbiology letters 2019 v.366 no.5 pp. -
ISSN:
1574-6968
Subject:
Enterococcus; Escherichia coli; Micrococcus luteus; Mycobacterium tuberculosis; Staphylococcus aureus; acetates; antibiotic resistance genes; bacteria; biosynthesis; gene expression regulation; mixed culture; models; mutation; phenotype; proteins; starvation; stress response; virulent strains
Abstract:
... Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress responses are critical for survival in non-pathogenic and pathogenic bacteria alike i ...
DOI:
10.1093/femsle/fnz054