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You searched for: Journal Journal of fish diseases Remove constraint Journal: Journal of fish diseases Subject term coat proteins Remove constraint Subject term: coat proteins
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1. Development of cross‐priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

Author:
Terawut Prasitporn; Saengchan Senapin; Akapon Vaniksampanna; Siwaporn Longyant; Parin Chaivisuthangkura
Source:
Journal of fish diseases 2021 v.44 no.9 pp. 1411-1422
ISSN:
0140-7775
Subject:
DNA; Infectious spleen and kidney necrosis virus; Lates calcarifer; Redspotted grouper nervous necrosis virus; coat proteins; colorimetry; cross reaction; fish; genes; temperature; uracil-DNA glycosylase; viruses
Abstract:
... Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil‐DNA glycosylase (UDG)‐supplemented cross‐priming amplification (UCPA) combined with a colorimetric detection method u ...
DOI:
10.1111/jfd.13448

2. Genetically modified attenuated salmonid alphavirus: A potential strategy for immunization of Atlantic salmon

Author:
Ida Aksnes; Stine Braaen; Turhan Markussen; Caroline Piercey Åkesson; Stephane Villoing; Espen Rimstad
Source:
Journal of fish diseases 2021 v.44 no.7 pp. 923-937
ISSN:
0140-7775
Subject:
Alphavirus; Salmo salar; capsid; coat proteins; fish; glycosylation; immunization; nuclear localization signals; pancreas disease; superinfection; viruses; weight gain
Abstract:
... Pancreas disease (PD) is a serious challenge in European salmonid aquaculture caused by salmonid alphavirus (SAV). In this study, we report the effect of immunization of Atlantic salmon with three attenuated infectious SAV3 strains with targeted mutations in a glycosylation site of the envelope E2 protein and/or in a nuclear localization signal in the capsid protein. In a pilot experiment, it was ...
DOI:
10.1111/jfd.13352

3. Infectious spleen and kidney necrosis virus‐associated large‐scale mortality in farmed giant gourami, Osphronemus goramy, in India

Author:
Thangaraj Raja Swaminathan; Nithianantham Sundar Raj; Prasannan Geetha Preena; Pravata Kumar Pradhan; Neeraj Sood; Rahul Girish Kumar; Arun Sudhagar; Naresh Kumar Sood
Source:
Journal of fish diseases 2021 v.44 no.12 pp. 2043-2053
ISSN:
0140-7775
Subject:
Gasterosteus aculeatus; Infectious spleen and kidney necrosis virus; Iridovirus; Osphronemus; Turbot reddish body iridovirus; aquaculture; biosecurity; cell lines; coat proteins; cytoplasm; freshwater; genes; kidneys; marine fish; mortality; necrosis; nucleocapsid; phylogeny; spleen; transmission electron microscopy; viruses; India; South East Asia
Abstract:
... Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South‐East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV) and scale drop disease virus (SDDV). ISKNV‐mediated infectious spleen and kidney ...
DOI:
10.1111/jfd.13519

4. Recombinant viral proteins delivered orally through inactivated bacterial cells induce protection in Macrobrachium rosenbergii (de Man) against White Tail Disease

Author:
Singaiah NaveenKumar; Praveen Rai; Indrani Karunasagar; Iddya Karunasagar
Source:
Journal of fish diseases 2021 v.44 no.5 pp. 601-612
ISSN:
0140-7775
Subject:
Macrobrachium rosenbergii; Macrobrachium rosenbergii nodavirus; aquaculture industry; capsid; coat proteins; fish; juveniles; mortality; oral administration; viral diseases of animals and humans; viruses
Abstract:
... White tail disease (WTD) is a disease of Macrobrachium rosenbergii caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) with the potential to devastate the aquaculture industry. The present study aimed to explore the possible protection of M. rosenbergii against the disease by oral administration of bacterially expressed recombinant capsid proteins of MrNV and XSV. Juve ...
DOI:
10.1111/jfd.13305

5. Characterization of the monoclonal antibody specific to the ORF72 protein of koi herpesvirus and cellular distribution analysis of the viral protein

Author:
Ching‐Wei Wu; Ting‐Yun Wu; Chih‐Jung Kuo; Yi‐Ping Lu; Maw‐Sheng Chien; Chienjin Huang
Source:
Journal of fish diseases 2020 v.43 no.7 pp. 791-799
ISSN:
0140-7775
Subject:
Cyprinid herpesvirus 3; Cyprinus carpio; amino acids; antibody specificity; coat proteins; disease severity; fish diseases; fluorescent antibody technique; genes; immunohistochemistry; koi; mitochondria; monoclonal antibodies; mortality; nuclear proteins; pathogens; tissue distribution; vaccines
Abstract:
... Koi herpesvirus (KHV) is an emerging pathogen of koi and common carp that causes a severe disease and mass mortality of infected fish. The KHV ORF72 protein is an important capsid protein that has been suggested to be a candidate for the development of diagnostic reagents and KHV vaccines. The purpose of this study was to clone and express the KHV ORF72 gene for further preparation of a specific m ...
DOI:
10.1111/jfd.13176

6. First genetic characterization of sturgeon mimiviruses in Ukraine

Author:
Yuriy Rud; Laurent Bigarré; Laurane Pallandre; François‐Xavier Briand; Leonid Buchatsky
Source:
Journal of fish diseases 2020 v.43 no.11 pp. 1391-1400
ISSN:
0140-7775
Subject:
Acipenser baerii; Acipenser gueldenstaedtii; Mimiviridae; alleles; coat proteins; farms; genetic relationships; melting; quantitative polymerase chain reaction; sturgeon; viruses; Ukraine
Abstract:
... A group of pathogenic nucleocytoplasmic large DNA viruses (NCLDVs) related to the Mimiviridae family infect farmed sturgeons across Europe, causing mild‐to‐severe losses. One of these viruses, Acipenser iridovirus‐European (AcIV‐E), was identified in six sturgeon species. During the 2018–2019 period, nine sick Siberian (A. baerii) and Russian (A. gueldenstaedtii) sturgeons were sampled in Ukrainia ...
DOI:
10.1111/jfd.13239

7. Amino acid changes in the capsid protein of a reassortant betanodavirus strain: Effect on viral replication in vitro and in vivo

Author:
Sandra Souto; José G. Olveira; Esther García‐Rosado; Carlos P. Dopazo; Isabel Bandín
Source:
Journal of fish diseases 2019 v.42 no.2 pp. 221-227
ISSN:
0140-7775
Subject:
Betanodavirus; Solea senegalensis; adsorption; amino acids; brain; capsid; coat proteins; mutants; mutation; virulence; virus replication
Abstract:
... Betanodavirus reassortant strains (RGNNV/SJNNV) isolated from Senegalese sole harbour an SJNNV capsid featuring several changes with respect to the SJNNV‐type strain, sharing three hallmark substitutions. Here, we have employed recombinant strains harbouring mutations in these positions (r20 and r20 + 247 + 270) and have demonstrated that the three substitutions affect different steps of the viral ...
DOI:
10.1111/jfd.12916

8. Comparative evaluation of MCP gene in worldwide strains of Megalocytivirus (Iridoviridae family) for early diagnostic marker

Author:
A Mishra; G‐H Nam; J‐A Gim; H‐E Lee; A Jo; D Yoon; S Oh; S Kim; A Kim; D‐H Kim; Y C Kim; H D Jeong; H‐J Cha; Y H Choi; H‐S Kim
Source:
Journal of fish diseases 2018 v.41 no.1 pp. 105-116
ISSN:
0140-7775
Subject:
DNA primers; Iridovirus; Megalocytivirus; Ranavirus; capsid; coat proteins; etiological agents; financial economics; fish; genes; mortality; phenotype; sequence analysis; viruses
Abstract:
... Members of the Iridoviridae family have been considered as aetiological agents of iridovirus diseases, causing fish mortalities and economic losses all over the world. Virus identification based on candidate gene sequencing is faster, more accurate and more reliable than other traditional phenotype methodologies. Iridoviridae viruses are covered by a protein shell (capsid) encoded by the important ...
DOI:
10.1111/jfd.12685

9. Molecular biology of Macrobrachium rosenbergii nodavirus infection in giant freshwater prawn

Author:
Chen‐Fei Low; Mohd Radzi Md Yusoff; Giva Kuppusamy; Nur Farahiyah Ahmad Nadzri
Source:
Journal of fish diseases 2018 v.41 no.12 pp. 1771-1781
ISSN:
0140-7775
Subject:
Macrobrachium rosenbergii; Macrobrachium rosenbergii nodavirus; antibodies; coat proteins; death; disease diagnosis; fish; medicinal properties; molecular biology; new genus; oligonucleotides; pathogenesis; pathogenicity; postlarvae; shrimp; shrimp culture; structural proteins; vaccine development; viral diseases of animals and humans; viral vaccines; virus assembly; viruses
Abstract:
... Macrobrachium rosenbergii nodavirus (MrNV) has been threatening the giant freshwater prawn aquaculture since 1997, causing white tail disease in the prawn species that leads to 100% lethality of the infected postlarvae. Comprehension of the viral infectivity and pathogenesis at molecular biology level has recently resolved the viral capsid protein and evidenced the significant difference in the vi ...
DOI:
10.1111/jfd.12895

10. A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

Author:
K I Kim; S D Hwang; M Y Cho; S H Jung; Y C Kim; H D Jeong
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1229-1233
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Siniperca scherzeri; Turbot reddish body iridovirus; adenosinetriphosphatase; coat proteins; freshwater fish; genes; genetic variation; phylogeny; quantitative polymerase chain reaction; spleen; Japan; Korean Peninsula
Abstract:
... An outbreak of a Megalocytivirus infection was found in the golden mandarin fish Siniperca scherzeri during September and October 2016, in Korea. Phylogeny and genetic diversity based on the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes showed a new strain. Designated as GMIV, this strain derived from the golden mandarin fish was suggested to belong to the red sea bream ir ...
DOI:
10.1111/jfd.12815

11. Betanodavirus: Dissection of the viral life cycle

Author:
C‐F Low; B Syarul Nataqain; H‐Y Chee; M Z H Rozaini; M Najiah
Source:
Journal of fish diseases 2017 v.40 no.11 pp. 1489-1496
ISSN:
0140-7775
Subject:
Betanodavirus; apoptosis; cell viability; coat proteins; death; encephalopathy; genome; genomics; marine fish; molecular biology; pathogens; retinal diseases; viral shedding; virion; viruses
Abstract:
... Progressive research has been recently made in dissecting the molecular biology of Betanodavirus life cycle, the causative pathogen of viral encephalopathy and retinopathy in economic important marine fish species. Establishment of betanodavirus infectious clone allows the manipulation of virus genome for functional genomic study, which elucidates the biological event of the viral life cycle at mo ...
DOI:
10.1111/jfd.12638

12. Molecular identification of iridoviruses infecting various sturgeon species in Europe

Author:
L Bigarré; M Lesne; A Lautraite; V Chesneau; A Leroux; M Jamin; P M Boitard; A Toffan; M Prearo; S Labrut; P Daniel
Source:
Journal of fish diseases 2017 v.40 no.1 pp. 105-118
ISSN:
0140-7775
Subject:
Acipenser gueldenstaedtii; Acipenser transmontanus; Huso huso; Iridovirus; coat proteins; epithelium; farms; genes; genetic heterogeneity; genotype; histology; morbidity; nucleotides; polymerase chain reaction; sturgeon; viruses; Canada; Europe
Abstract:
... Iridoviridae are known to cause disease in sturgeons in North America. Here, histological and molecular methods were used to screen for this family of virus in sturgeons from various European farms with low‐to‐high morbidity. Some histological samples revealed basophilic cells in the gill and labial epithelia, strongly suggesting the accumulation of iridovirus particles. Newly developed generic PC ...
DOI:
10.1111/jfd.12498

13. Outbreaks of ulcerative disease associated with ranavirus infection in barcoo grunter, Scortum barcoo (McCulloch & Waite)

Author:
P Kayansamruaj; A Rangsichol; H T Dong; C Rodkhum; M Maita; T Katagiri; N Pirarat
Source:
Journal of fish diseases 2017 v.40 no.10 pp. 1341-1350
ISSN:
0140-7775
Subject:
Micropterus salmoides; Santee-Cooper ranavirus; Scortum barcoo; coat proteins; farms; fish; genes; hemorrhage; inclusion bodies; kidneys; mortality; muscles; necrosis; nucleotide sequences; polymerase chain reaction; skin lesions; spleen; viruses; Thailand
Abstract:
... In 2013, an outbreak of ulcerative disease associated with ranavirus infection occurred in barcoo grunter, Scortum barcoo (McCulloch & Waite), farms in Thailand. Affected fish exhibited extensive haemorrhage and ulceration on skin and muscle. Microscopically, the widespread haemorrhagic ulceration and necrosis were noted in gill, spleen and kidney with the presence of intracytoplasmic eosinophilic ...
DOI:
10.1111/jfd.12606

14. Rapid and sensitive detection of redspotted grouper nervous necrosis virus (RGNNV) infection by aptamer–coat protein–aptamer sandwich enzyme‐linked apta‐sorbent assay (ELASA)

Author:
L Zhou; P Li; S Ni; Y Yu; M Yang; S Wei; Q Qin
Source:
Journal of fish diseases 2017 v.40 no.12 pp. 1831-1838
ISSN:
0140-7775
Subject:
Redspotted grouper nervous necrosis virus; aquaculture; brain; coat proteins; equipment; farmers; grouper; oligonucleotides; pathogens; reverse transcriptase polymerase chain reaction; streptavidin; temperature; tissues; virion
Abstract:
... Redspotted grouper nervous necrosis virus (RGNNV) is one of the most devastating pathogens in the aquaculture of the grouper, Epinephlus sp., worldwide. The early and rapid diagnosis of RGNNV is important for the prevention of RGNNV infection. In this study, an aptamer (A10)‐based sandwich enzyme‐linked apta‐sorbent assay (ELASA) was developed for RGNNV diagnosis. This sandwich ELASA showed high s ...
DOI:
10.1111/jfd.12656

15. Developing immunological methods for detecting Macrobrachium rosenbergii nodavirus and extra small virus using a recombinant protein preparation

Author:
C‐S Wang; C‐Y Chang; C‐M Wen
Source:
Journal of fish diseases 2016 v.39 no.6 pp. 715-727
ISSN:
0140-7775
Subject:
Betanodavirus; Escherichia coli; Macrobrachium rosenbergii nodavirus; Western blotting; White spot syndrome virus; coat proteins; glutathione transferase; grouper; immunohistochemistry; molecular weight; muscle tissues; nucleotide sequences; polyclonal antibodies; recombinant proteins; reverse transcriptase polymerase chain reaction; shrimp; transcription (genetics); viral diseases of animals and humans; viruses
Abstract:
... Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) have been identified as the causative agents for white tail disease (WTD) of M. rosenbergii. In this study, the gene sequences encoding MrNV and XSV capsid proteins were separately ligated into the pGEX‐4T‐3 expression vector and transformed into Escherichia coli. After induction, glutathione‐S‐transferase (GST)‐tagged MrNV and ...
DOI:
10.1111/jfd.12404

16. Low pathogenicity of flounder iridovirus (FLIV) and the absence of cross‐protection between FLIV and rock bream iridovirus

Author:
M H Jung; J Lee; S J Jung
Source:
Journal of fish diseases 2016 v.39 no.11 pp. 1325-1333
ISSN:
0140-7775
Subject:
Iridovirus; Paralichthys olivaceus; Rock bream iridovirus; bream; coat proteins; cross immunity; flounder; genes; genetically modified organisms; genotype; hosts; marine fish; mortality; pathogenicity; spleen; stocking rate; temperature; virus replication; viruses
Abstract:
... The genus Megalocytivirus is known to infect a wide range of cultured marine fish. In this study, we examined the pathogenicity of FLIV (Megalocytivirus from olive flounder, genotype III) and RBIV (Megalocytivirus from rock bream, genotype I) to their homologous and heterologous host species. Olive flounder (7.5 ± 1.3 cm) injected with FLIV [major capsid protein (MCP) gene copies, 6.8 × 10³–6.5 ×  ...
DOI:
10.1111/jfd.12459

17. Quantitative immunoenzymatic detection of viral encephalopathy and retinopathy virus (betanodavirus) in sea bass Dicentrarchus labrax

Author:
N Nuñez‐Ortiz; V Stocchi; A Toffan; F Pascoli; N Sood; F Buonocore; S Picchietti; C Papeschi; A R Taddei; K D Thompson; G Scapigliati
Source:
Journal of fish diseases 2016 v.39 no.7 pp. 821-831
ISSN:
0140-7775
Subject:
Dicentrarchus labrax; Redspotted grouper nervous necrosis virus; bass; brain; coat proteins; encephalopathy; enzyme-linked immunosorbent assay; epitopes; farmed animal species; genotype; immunoglobulin M; monoclonal antibodies; polyclonal antibodies; polymerase chain reaction; retinal diseases; viral load; viruses; Europe; Mediterranean region
Abstract:
... Viral encephalopathy and retinopathy disease caused by betanodavirus, genus of the family Nodaviridae, affects marine, wild and farmed species including sea bass, one of the most important farmed species in Europe. This work describes a reliable and sensitive indirect ELISA assay to detect betanodavirus in biological samples using a polyclonal antiserum (pAb 283) against the 283/I09 virus strain, ...
DOI:
10.1111/jfd.12415

18. Detection and characterization of viruses of the genus Megalocytivirus in ornamental fish imported into an Australian border quarantine premises: an emerging risk to national biosecurity

Author:
D Nolan; F Stephens; M Crockford; J B Jones; M Snow
Source:
Journal of fish diseases 2015 v.38 no.2 pp. 187-195
ISSN:
0140-7775
Subject:
Megalocytivirus; aquaculture tanks; biosecurity; coat proteins; genotype; host range; inclusion bodies; mortality; ornamental fish; pathogenicity; phylogeny; polymerase chain reaction; quarantine; risk; sequence analysis; viruses; Australia; Malaysia; Singapore; Sri Lanka
Abstract:
... This report documents an emerging trend of identification of Megalocytivirus‐like inclusions in a range of ornamental fish species intercepted during quarantine detention at the Australian border. From September 2012 to February 2013, 5 species of fish that had suffered mortality levels in excess of 25% whilst in the post‐entry quarantine and had Megalocytivirus‐like inclusion bodies in histologic ...
DOI:
10.1111/jfd.12222

19. Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm‐raised tilapia Oreochromis niloticus (L.) in Thailand

Author:
J Keawcharoen; S Techangamsuwan; A Ponpornpisit; E D Lombardini; T Patchimasiri; N Pirarat
Source:
Journal of fish diseases 2015 v.38 no.1 pp. 49-54
ISSN:
0140-7775
Subject:
Oreochromis niloticus; Redspotted grouper nervous necrosis virus; coat proteins; disease outbreaks; farmed fish; genes; immunohistochemistry; larvae; necrosis; nucleotide sequences; phylogeny; viruses; Thailand
Abstract:
... Betanodavirus infection was diagnosed in larvae of farm‐raised tilapia Oreochromis niloticus (L.), in central Thailand. Extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. Molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. The ...
DOI:
10.1111/jfd.12200

20. Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China

Author:
X Huang; Y Huang; L Xu; S Wei; Z Ouyang; J Feng; Q Qin
Source:
Journal of fish diseases 2015 v.38 no.4 pp. 379-387
ISSN:
0140-7775
Subject:
DNA-directed DNA polymerase; Epinephelus; Megalocytivirus; Ranavirus; coat proteins; cytoplasm; genes; giant cells; grouper; inclusion bodies; mariculture; membrane proteins; pathogens; phylogeny; ribonucleotide reductase; viruses; China; South East Asia
Abstract:
... Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South‐East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus and Ranavirus, have been well characterized in recent years. To date, few data on lymphocystis disease in grouper which caused by lymphocystis disease virus (LCDV) were described. Here, a nov ...
DOI:
10.1111/jfd.12244