U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal Journal of fish diseases Remove constraint Journal: Journal of fish diseases Subject term coat proteins Remove constraint Subject term: coat proteins
Start Over

Search

37  Search Results

1 - 37 of 37
Number of results to display per page

Search Results

1. Development of cross‐priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

Author:
Terawut Prasitporn; Saengchan Senapin; Akapon Vaniksampanna; Siwaporn Longyant; Parin Chaivisuthangkura
Source:
Journal of fish diseases 2021 v.44 no.9 pp. 1411-1422
ISSN:
0140-7775
Subject:
DNA; Infectious spleen and kidney necrosis virus; Lates calcarifer; Redspotted grouper nervous necrosis virus; coat proteins; colorimetry; cross reaction; fish; genes; temperature; uracil-DNA glycosylase; viruses
Abstract:
... Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil‐DNA glycosylase (UDG)‐supplemented cross‐priming amplification (UCPA) combined with a colorimetric detection method u ...
DOI:
10.1111/jfd.13448

2. Genetically modified attenuated salmonid alphavirus: A potential strategy for immunization of Atlantic salmon

Author:
Ida Aksnes; Stine Braaen; Turhan Markussen; Caroline Piercey Åkesson; Stephane Villoing; Espen Rimstad
Source:
Journal of fish diseases 2021 v.44 no.7 pp. 923-937
ISSN:
0140-7775
Subject:
Alphavirus; Salmo salar; capsid; coat proteins; fish; glycosylation; immunization; nuclear localization signals; pancreas disease; superinfection; viruses; weight gain
Abstract:
... Pancreas disease (PD) is a serious challenge in European salmonid aquaculture caused by salmonid alphavirus (SAV). In this study, we report the effect of immunization of Atlantic salmon with three attenuated infectious SAV3 strains with targeted mutations in a glycosylation site of the envelope E2 protein and/or in a nuclear localization signal in the capsid protein. In a pilot experiment, it was ...
DOI:
10.1111/jfd.13352

3. Infectious spleen and kidney necrosis virus‐associated large‐scale mortality in farmed giant gourami, Osphronemus goramy, in India

Author:
Thangaraj Raja Swaminathan; Nithianantham Sundar Raj; Prasannan Geetha Preena; Pravata Kumar Pradhan; Neeraj Sood; Rahul Girish Kumar; Arun Sudhagar; Naresh Kumar Sood
Source:
Journal of fish diseases 2021 v.44 no.12 pp. 2043-2053
ISSN:
0140-7775
Subject:
Gasterosteus aculeatus; Infectious spleen and kidney necrosis virus; Iridovirus; Osphronemus; Turbot reddish body iridovirus; aquaculture; biosecurity; cell lines; coat proteins; cytoplasm; freshwater; genes; kidneys; marine fish; mortality; necrosis; nucleocapsid; phylogeny; spleen; transmission electron microscopy; viruses; India; South East Asia
Abstract:
... Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South‐East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV) and scale drop disease virus (SDDV). ISKNV‐mediated infectious spleen and kidney ...
DOI:
10.1111/jfd.13519

4. Recombinant viral proteins delivered orally through inactivated bacterial cells induce protection in Macrobrachium rosenbergii (de Man) against White Tail Disease

Author:
Singaiah NaveenKumar; Praveen Rai; Indrani Karunasagar; Iddya Karunasagar
Source:
Journal of fish diseases 2021 v.44 no.5 pp. 601-612
ISSN:
0140-7775
Subject:
Macrobrachium rosenbergii; Macrobrachium rosenbergii nodavirus; aquaculture industry; capsid; coat proteins; fish; juveniles; mortality; oral administration; viral diseases of animals and humans; viruses
Abstract:
... White tail disease (WTD) is a disease of Macrobrachium rosenbergii caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) with the potential to devastate the aquaculture industry. The present study aimed to explore the possible protection of M. rosenbergii against the disease by oral administration of bacterially expressed recombinant capsid proteins of MrNV and XSV. Juve ...
DOI:
10.1111/jfd.13305

5. Characterization of the monoclonal antibody specific to the ORF72 protein of koi herpesvirus and cellular distribution analysis of the viral protein

Author:
Ching‐Wei Wu; Ting‐Yun Wu; Chih‐Jung Kuo; Yi‐Ping Lu; Maw‐Sheng Chien; Chienjin Huang
Source:
Journal of fish diseases 2020 v.43 no.7 pp. 791-799
ISSN:
0140-7775
Subject:
Cyprinid herpesvirus 3; Cyprinus carpio; amino acids; antibody specificity; coat proteins; disease severity; fish diseases; fluorescent antibody technique; genes; immunohistochemistry; koi; mitochondria; monoclonal antibodies; mortality; nuclear proteins; pathogens; tissue distribution; vaccines
Abstract:
... Koi herpesvirus (KHV) is an emerging pathogen of koi and common carp that causes a severe disease and mass mortality of infected fish. The KHV ORF72 protein is an important capsid protein that has been suggested to be a candidate for the development of diagnostic reagents and KHV vaccines. The purpose of this study was to clone and express the KHV ORF72 gene for further preparation of a specific m ...
DOI:
10.1111/jfd.13176

6. First genetic characterization of sturgeon mimiviruses in Ukraine

Author:
Yuriy Rud; Laurent Bigarré; Laurane Pallandre; François‐Xavier Briand; Leonid Buchatsky
Source:
Journal of fish diseases 2020 v.43 no.11 pp. 1391-1400
ISSN:
0140-7775
Subject:
Acipenser baerii; Acipenser gueldenstaedtii; Mimiviridae; alleles; coat proteins; farms; genetic relationships; melting; quantitative polymerase chain reaction; sturgeon; viruses; Ukraine
Abstract:
... A group of pathogenic nucleocytoplasmic large DNA viruses (NCLDVs) related to the Mimiviridae family infect farmed sturgeons across Europe, causing mild‐to‐severe losses. One of these viruses, Acipenser iridovirus‐European (AcIV‐E), was identified in six sturgeon species. During the 2018–2019 period, nine sick Siberian (A. baerii) and Russian (A. gueldenstaedtii) sturgeons were sampled in Ukrainia ...
DOI:
10.1111/jfd.13239

7. Amino acid changes in the capsid protein of a reassortant betanodavirus strain: Effect on viral replication in vitro and in vivo

Author:
Sandra Souto; José G. Olveira; Esther García‐Rosado; Carlos P. Dopazo; Isabel Bandín
Source:
Journal of fish diseases 2019 v.42 no.2 pp. 221-227
ISSN:
0140-7775
Subject:
Betanodavirus; Solea senegalensis; adsorption; amino acids; brain; capsid; coat proteins; mutants; mutation; virulence; virus replication
Abstract:
... Betanodavirus reassortant strains (RGNNV/SJNNV) isolated from Senegalese sole harbour an SJNNV capsid featuring several changes with respect to the SJNNV‐type strain, sharing three hallmark substitutions. Here, we have employed recombinant strains harbouring mutations in these positions (r20 and r20 + 247 + 270) and have demonstrated that the three substitutions affect different steps of the viral ...
DOI:
10.1111/jfd.12916

8. Comparative evaluation of MCP gene in worldwide strains of Megalocytivirus (Iridoviridae family) for early diagnostic marker

Author:
A Mishra; G‐H Nam; J‐A Gim; H‐E Lee; A Jo; D Yoon; S Oh; S Kim; A Kim; D‐H Kim; Y C Kim; H D Jeong; H‐J Cha; Y H Choi; H‐S Kim
Source:
Journal of fish diseases 2018 v.41 no.1 pp. 105-116
ISSN:
0140-7775
Subject:
DNA primers; Iridovirus; Megalocytivirus; Ranavirus; capsid; coat proteins; etiological agents; financial economics; fish; genes; mortality; phenotype; sequence analysis; viruses
Abstract:
... Members of the Iridoviridae family have been considered as aetiological agents of iridovirus diseases, causing fish mortalities and economic losses all over the world. Virus identification based on candidate gene sequencing is faster, more accurate and more reliable than other traditional phenotype methodologies. Iridoviridae viruses are covered by a protein shell (capsid) encoded by the important ...
DOI:
10.1111/jfd.12685

9. Molecular biology of Macrobrachium rosenbergii nodavirus infection in giant freshwater prawn

Author:
Chen‐Fei Low; Mohd Radzi Md Yusoff; Giva Kuppusamy; Nur Farahiyah Ahmad Nadzri
Source:
Journal of fish diseases 2018 v.41 no.12 pp. 1771-1781
ISSN:
0140-7775
Subject:
Macrobrachium rosenbergii; Macrobrachium rosenbergii nodavirus; antibodies; coat proteins; death; disease diagnosis; fish; medicinal properties; molecular biology; new genus; oligonucleotides; pathogenesis; pathogenicity; postlarvae; shrimp; shrimp culture; structural proteins; vaccine development; viral diseases of animals and humans; viral vaccines; virus assembly; viruses
Abstract:
... Macrobrachium rosenbergii nodavirus (MrNV) has been threatening the giant freshwater prawn aquaculture since 1997, causing white tail disease in the prawn species that leads to 100% lethality of the infected postlarvae. Comprehension of the viral infectivity and pathogenesis at molecular biology level has recently resolved the viral capsid protein and evidenced the significant difference in the vi ...
DOI:
10.1111/jfd.12895

10. A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

Author:
K I Kim; S D Hwang; M Y Cho; S H Jung; Y C Kim; H D Jeong
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1229-1233
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Siniperca scherzeri; Turbot reddish body iridovirus; adenosinetriphosphatase; coat proteins; freshwater fish; genes; genetic variation; phylogeny; quantitative polymerase chain reaction; spleen; Japan; Korean Peninsula
Abstract:
... An outbreak of a Megalocytivirus infection was found in the golden mandarin fish Siniperca scherzeri during September and October 2016, in Korea. Phylogeny and genetic diversity based on the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes showed a new strain. Designated as GMIV, this strain derived from the golden mandarin fish was suggested to belong to the red sea bream ir ...
DOI:
10.1111/jfd.12815

11. Betanodavirus: Dissection of the viral life cycle

Author:
C‐F Low; B Syarul Nataqain; H‐Y Chee; M Z H Rozaini; M Najiah
Source:
Journal of fish diseases 2017 v.40 no.11 pp. 1489-1496
ISSN:
0140-7775
Subject:
Betanodavirus; apoptosis; cell viability; coat proteins; death; encephalopathy; genome; genomics; marine fish; molecular biology; pathogens; retinal diseases; viral shedding; virion; viruses
Abstract:
... Progressive research has been recently made in dissecting the molecular biology of Betanodavirus life cycle, the causative pathogen of viral encephalopathy and retinopathy in economic important marine fish species. Establishment of betanodavirus infectious clone allows the manipulation of virus genome for functional genomic study, which elucidates the biological event of the viral life cycle at mo ...
DOI:
10.1111/jfd.12638

12. Molecular identification of iridoviruses infecting various sturgeon species in Europe

Author:
L Bigarré; M Lesne; A Lautraite; V Chesneau; A Leroux; M Jamin; P M Boitard; A Toffan; M Prearo; S Labrut; P Daniel
Source:
Journal of fish diseases 2017 v.40 no.1 pp. 105-118
ISSN:
0140-7775
Subject:
Acipenser gueldenstaedtii; Acipenser transmontanus; Huso huso; Iridovirus; coat proteins; epithelium; farms; genes; genetic heterogeneity; genotype; histology; morbidity; nucleotides; polymerase chain reaction; sturgeon; viruses; Canada; Europe
Abstract:
... Iridoviridae are known to cause disease in sturgeons in North America. Here, histological and molecular methods were used to screen for this family of virus in sturgeons from various European farms with low‐to‐high morbidity. Some histological samples revealed basophilic cells in the gill and labial epithelia, strongly suggesting the accumulation of iridovirus particles. Newly developed generic PC ...
DOI:
10.1111/jfd.12498

13. Outbreaks of ulcerative disease associated with ranavirus infection in barcoo grunter, Scortum barcoo (McCulloch & Waite)

Author:
P Kayansamruaj; A Rangsichol; H T Dong; C Rodkhum; M Maita; T Katagiri; N Pirarat
Source:
Journal of fish diseases 2017 v.40 no.10 pp. 1341-1350
ISSN:
0140-7775
Subject:
Micropterus salmoides; Santee-Cooper ranavirus; Scortum barcoo; coat proteins; farms; fish; genes; hemorrhage; inclusion bodies; kidneys; mortality; muscles; necrosis; nucleotide sequences; polymerase chain reaction; skin lesions; spleen; viruses; Thailand
Abstract:
... In 2013, an outbreak of ulcerative disease associated with ranavirus infection occurred in barcoo grunter, Scortum barcoo (McCulloch & Waite), farms in Thailand. Affected fish exhibited extensive haemorrhage and ulceration on skin and muscle. Microscopically, the widespread haemorrhagic ulceration and necrosis were noted in gill, spleen and kidney with the presence of intracytoplasmic eosinophilic ...
DOI:
10.1111/jfd.12606

14. Rapid and sensitive detection of redspotted grouper nervous necrosis virus (RGNNV) infection by aptamer–coat protein–aptamer sandwich enzyme‐linked apta‐sorbent assay (ELASA)

Author:
L Zhou; P Li; S Ni; Y Yu; M Yang; S Wei; Q Qin
Source:
Journal of fish diseases 2017 v.40 no.12 pp. 1831-1838
ISSN:
0140-7775
Subject:
Redspotted grouper nervous necrosis virus; aquaculture; brain; coat proteins; equipment; farmers; grouper; oligonucleotides; pathogens; reverse transcriptase polymerase chain reaction; streptavidin; temperature; tissues; virion
Abstract:
... Redspotted grouper nervous necrosis virus (RGNNV) is one of the most devastating pathogens in the aquaculture of the grouper, Epinephlus sp., worldwide. The early and rapid diagnosis of RGNNV is important for the prevention of RGNNV infection. In this study, an aptamer (A10)‐based sandwich enzyme‐linked apta‐sorbent assay (ELASA) was developed for RGNNV diagnosis. This sandwich ELASA showed high s ...
DOI:
10.1111/jfd.12656

15. Developing immunological methods for detecting Macrobrachium rosenbergii nodavirus and extra small virus using a recombinant protein preparation

Author:
C‐S Wang; C‐Y Chang; C‐M Wen
Source:
Journal of fish diseases 2016 v.39 no.6 pp. 715-727
ISSN:
0140-7775
Subject:
Betanodavirus; Escherichia coli; Macrobrachium rosenbergii nodavirus; Western blotting; White spot syndrome virus; coat proteins; glutathione transferase; grouper; immunohistochemistry; molecular weight; muscle tissues; nucleotide sequences; polyclonal antibodies; recombinant proteins; reverse transcriptase polymerase chain reaction; shrimp; transcription (genetics); viral diseases of animals and humans; viruses
Abstract:
... Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) have been identified as the causative agents for white tail disease (WTD) of M. rosenbergii. In this study, the gene sequences encoding MrNV and XSV capsid proteins were separately ligated into the pGEX‐4T‐3 expression vector and transformed into Escherichia coli. After induction, glutathione‐S‐transferase (GST)‐tagged MrNV and ...
DOI:
10.1111/jfd.12404

16. Low pathogenicity of flounder iridovirus (FLIV) and the absence of cross‐protection between FLIV and rock bream iridovirus

Author:
M H Jung; J Lee; S J Jung
Source:
Journal of fish diseases 2016 v.39 no.11 pp. 1325-1333
ISSN:
0140-7775
Subject:
Iridovirus; Paralichthys olivaceus; Rock bream iridovirus; bream; coat proteins; cross immunity; flounder; genes; genetically modified organisms; genotype; hosts; marine fish; mortality; pathogenicity; spleen; stocking rate; temperature; virus replication; viruses
Abstract:
... The genus Megalocytivirus is known to infect a wide range of cultured marine fish. In this study, we examined the pathogenicity of FLIV (Megalocytivirus from olive flounder, genotype III) and RBIV (Megalocytivirus from rock bream, genotype I) to their homologous and heterologous host species. Olive flounder (7.5 ± 1.3 cm) injected with FLIV [major capsid protein (MCP) gene copies, 6.8 × 10³–6.5 ×  ...
DOI:
10.1111/jfd.12459

17. Quantitative immunoenzymatic detection of viral encephalopathy and retinopathy virus (betanodavirus) in sea bass Dicentrarchus labrax

Author:
N Nuñez‐Ortiz; V Stocchi; A Toffan; F Pascoli; N Sood; F Buonocore; S Picchietti; C Papeschi; A R Taddei; K D Thompson; G Scapigliati
Source:
Journal of fish diseases 2016 v.39 no.7 pp. 821-831
ISSN:
0140-7775
Subject:
Dicentrarchus labrax; Redspotted grouper nervous necrosis virus; bass; brain; coat proteins; encephalopathy; enzyme-linked immunosorbent assay; epitopes; farmed animal species; genotype; immunoglobulin M; monoclonal antibodies; polyclonal antibodies; polymerase chain reaction; retinal diseases; viral load; viruses; Europe; Mediterranean region
Abstract:
... Viral encephalopathy and retinopathy disease caused by betanodavirus, genus of the family Nodaviridae, affects marine, wild and farmed species including sea bass, one of the most important farmed species in Europe. This work describes a reliable and sensitive indirect ELISA assay to detect betanodavirus in biological samples using a polyclonal antiserum (pAb 283) against the 283/I09 virus strain, ...
DOI:
10.1111/jfd.12415

18. Detection and characterization of viruses of the genus Megalocytivirus in ornamental fish imported into an Australian border quarantine premises: an emerging risk to national biosecurity

Author:
D Nolan; F Stephens; M Crockford; J B Jones; M Snow
Source:
Journal of fish diseases 2015 v.38 no.2 pp. 187-195
ISSN:
0140-7775
Subject:
Megalocytivirus; aquaculture tanks; biosecurity; coat proteins; genotype; host range; inclusion bodies; mortality; ornamental fish; pathogenicity; phylogeny; polymerase chain reaction; quarantine; risk; sequence analysis; viruses; Australia; Malaysia; Singapore; Sri Lanka
Abstract:
... This report documents an emerging trend of identification of Megalocytivirus‐like inclusions in a range of ornamental fish species intercepted during quarantine detention at the Australian border. From September 2012 to February 2013, 5 species of fish that had suffered mortality levels in excess of 25% whilst in the post‐entry quarantine and had Megalocytivirus‐like inclusion bodies in histologic ...
DOI:
10.1111/jfd.12222

19. Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm‐raised tilapia Oreochromis niloticus (L.) in Thailand

Author:
J Keawcharoen; S Techangamsuwan; A Ponpornpisit; E D Lombardini; T Patchimasiri; N Pirarat
Source:
Journal of fish diseases 2015 v.38 no.1 pp. 49-54
ISSN:
0140-7775
Subject:
Oreochromis niloticus; Redspotted grouper nervous necrosis virus; coat proteins; disease outbreaks; farmed fish; genes; immunohistochemistry; larvae; necrosis; nucleotide sequences; phylogeny; viruses; Thailand
Abstract:
... Betanodavirus infection was diagnosed in larvae of farm‐raised tilapia Oreochromis niloticus (L.), in central Thailand. Extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. Molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. The ...
DOI:
10.1111/jfd.12200

20. Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China

Author:
X Huang; Y Huang; L Xu; S Wei; Z Ouyang; J Feng; Q Qin
Source:
Journal of fish diseases 2015 v.38 no.4 pp. 379-387
ISSN:
0140-7775
Subject:
DNA-directed DNA polymerase; Epinephelus; Megalocytivirus; Ranavirus; coat proteins; cytoplasm; genes; giant cells; grouper; inclusion bodies; mariculture; membrane proteins; pathogens; phylogeny; ribonucleotide reductase; viruses; China; South East Asia
Abstract:
... Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South‐East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus and Ranavirus, have been well characterized in recent years. To date, few data on lymphocystis disease in grouper which caused by lymphocystis disease virus (LCDV) were described. Here, a nov ...
DOI:
10.1111/jfd.12244

21. Isolation and characterization of a ranavirus from koi, Cyprinus carpio L., experiencing mass mortalities in India

Author:
M R George; K R John; M M Mansoor; R Saravanakumar; P Sundar; V Pradeep
Source:
Journal of fish diseases 2015 v.38 no.4 pp. 389-403
ISSN:
0140-7775
Subject:
Cyprinus carpio; Santee-Cooper ranavirus; amino acid sequences; cell culture; cell membranes; coat proteins; electron microscopy; fish farms; genes; kidneys; koi; mortality; pathogenicity; polymerase chain reaction; sequence analysis; swimming; virus transmission; viruses; India; North America
Abstract:
... We investigated mass mortalities of koi, Cyprinus carpio Linnaeus, 1758, experienced in South Indian fish farms by virus isolation, electron microscopy, PCR detection, sequencing of capsid protein gene and transmission studies. Samples of moribund koi brought to the laboratory suffered continuous mortality exhibiting swimming abnormalities, intermittent surfacing and skin darkening. Irido‐like vir ...
DOI:
10.1111/jfd.12246

22. Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in Peninsular Malaysia

Author:
K Subramaniam; M Shariff; A R Omar; M Hair‐Bejo; B L Ong
Source:
Journal of fish diseases 2014 v.37 no.7 pp. 609-618
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; breeding; coat proteins; enzymes; freshwater; genes; genotype; nucleotide sequences; ornamental fish; phylogeny; polymerase chain reaction; sequence analysis; turbot; viruses; Malaysia
Abstract:
... ‘Gold standard’ OIE reference PCR assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (ISKNV) in freshwater ornamental fish from Malaysia. From total of 210 ornamental fish samples representing 14 species, ISKNV was detected in 36 samples representing 5 fish species. All positive cases did not show any clinical signs of ISKNV. Three restriction enzymes analyses ...
DOI:
10.1111/jfd.12152
PubMed:
23952914

23. Production of recombinant capsid protein of Macrobrachium rosenbergii nodavirus (r‐MCP43) of giant freshwater prawn, M. rosenbergii (de Man) for immunological diagnostic methods

Author:
M A Farook; N Madan; G Taju; S Abdul Majeed; K S N Nambi; N Sundar Raj; S Vimal; A S Sahul Hameed
Source:
Journal of fish diseases 2014 v.37 no.8 pp. 703-710
ISSN:
0140-7775
Subject:
Escherichia coli; Macrobrachium rosenbergii; Nodaviridae; Western blotting; adults; antiserum; coat proteins; diagnostic techniques; enzyme-linked immunosorbent assay; genes; hatcheries; rabbits; reverse transcriptase polymerase chain reaction; shrimp; sodium chloride; viruses
Abstract:
... White tail disease (WTD) caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) is a serious problem in prawn hatcheries. The gene for capsid protein of MrNV (MCP43) was cloned into pRSET B expression vector. The MCP43 protein was expressed as a protein with a 6‐histidine tag in Escherichia coli GJ1158 with NaCl induction. This recombinant protein, which was used to raise ...
DOI:
10.1111/jfd.12156

24. Tissue distribution of hepatopancreatic parvo‐like virus of shrimp in freshwater rice‐field crab, Paratelphusa hydrodomous (Herbst)

Author:
N Madan; T Rajkumar; N Sundar Raj; M A Farook; K S N Nambi; S Abdul Majeed; A S Sahul Hameed
Source:
Journal of fish diseases 2014 v.37 no.11 pp. 969-980
ISSN:
0140-7775
Subject:
Penaeus monodon; Western blotting; bioassays; coat proteins; crabs; enzyme-linked immunosorbent assay; freshwater; genes; inoculum; intramuscular injection; mortality; reverse transcriptase polymerase chain reaction; shrimp; tissue distribution; viruses
Abstract:
... An attempt was made to determine the replication efficiency of hepatopancreatic parvo‐like virus (HPV) of shrimp in different organs of freshwater rice‐field crab Paratelphusa hydrodomous (Herbst) using bioassay, PCR, RT‐PCR, ELISA, Western blot and q‐PCR analyses. Another attempt was made to use this crab as an alternative to penaeid shrimp for the large‐scale production of HPV. This crab was fou ...
DOI:
10.1111/jfd.12183
PubMed:
24117535

25. Detection of antigenic proteins expressed by lymphocystis virus as vaccine candidates in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)

Author:
Jang, H.B.; Kim, Y.R.; Cha, I.S.; Noh, S.W.; Park, S.B.; Ohtani, M.; Hikima, J.; Aoki, T.; Jung, T.S.
Source:
Journal of fish diseases 2011 v.34 no.7 pp. 555-562
ISSN:
0140-7775
Subject:
Lymphocystivirus; Paralichthys olivaceus; antigen-antibody reactions; antiserum; coat proteins; fish; gel electrophoresis; infection; lasers; mass spectrometry; vaccination; vaccines; viral antigens; virulence; viruses
Abstract:
... Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host-derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus, we analysed the viral proteins responsible for its virulence by applying im ...
DOI:
10.1111/j.1365-2761.2011.01268.x
PubMed:
21675997

26. Development of real-time PCR assays for detection of megalocytiviruses in imported ornamental fish

Author:
Gias, E.; Johnston, C.; Keeling, S.; Spence, R.P.; McDonald, W.L.
Source:
Journal of fish diseases 2011 v.34 no.8 pp. 609-618
ISSN:
0140-7775
Subject:
DNA; Iridovirus; Trichogaster trichopterus; analytical specificity; coat proteins; detection limit; farmed fish; genes; imports; ornamental fish; polymerase chain reaction; viruses; Australia; New Zealand
Abstract:
... Megalocytiviruses have been associated globally with severe systemic disease and economic loss in farmed food fish and ornamental fish. The viruses have been spread internationally by translocation of live fish. In New Zealand, megalocytiviruses are regarded as exotic. A potential pathway for introduction has been identified, namely imported ornamental fish. In the present study, real-time PCR ass ...
DOI:
10.1111/j.1365-2761.2011.01274.x
PubMed:
21762172

27. Investigation of ornamental fish entering the EU for the presence of ranaviruses

Author:
Vesely, T.; Cinkova, K.; Reschova, S.; Gobbo, F.; Ariel, E.; Vicenova, M.; Pokorova, D.; Kulich, P.; Bovo, G.
Source:
Journal of fish diseases 2011 v.34 no.2 pp. 159-166
ISSN:
0140-7775
Subject:
DNA-directed DNA polymerase; European Union; Iridovirus; Ranavirus; coat proteins; disease prevalence; fish industry; imports; international trade; nucleotide sequences; ornamental fish; polymerase chain reaction; surveys; transmission electron microscopy; viruses
Abstract:
... A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, ...
DOI:
10.1111/j.1365-2761.2010.01224.x
PubMed:
21241323

28. Complete sequencing of Tunisian redspotted grouper nervous necrosis virus betanodavirus capsid gene and RNA-dependent RNA polymerase gene

Author:
Chérif, N.; Gagné, N.; Groman, D.; Kibenge, F.; Iwamoto, T.; Yason, C.; Hammami, S.
Source:
Journal of fish diseases 2010 v.33 no.3 pp. 231-240
ISSN:
0140-7775
Subject:
RNA; RNA-directed RNA polymerase; amino acids; capsid; coat proteins; farmed animal species; fish larvae; genes; genotype; juveniles; marine fish; open reading frames; phylogeny
Abstract:
... Finfish nodaviruses (betanodaviruses) can cause highly destructive infections in numerous species of farmed marine fish larvae and juveniles worldwide. The betanodavirus genome consists of two single-stranded positive-sense RNA molecules (RNA1 and RNA2). The virus can be classified into four genotypes based on the partial sequences of the coat protein (CP) gene (T2 and T4 regions). Currently, geno ...
DOI:
10.1111/j.1365-2761.2009.01116.x
PubMed:
19912455

29. Iridovirus infections in finfish - critical review with emphasis on ranaviruses

Author:
Whittington, R.J.; Becker, J.A.; Dennis, M.M.
Source:
Journal of fish diseases 2010 v.33 no.2 pp. 95-122
ISSN:
0140-7775
Subject:
European catfish virus; Iridovirus; Santee-Cooper ranavirus; Singapore grouper iridovirus; amphibians; animal health; coat proteins; disease reservoirs; epizootic diseases; financial economics; genes; host specificity; hosts; issues and policy; monitoring; mortality; ornamental fish; pathogens; reptiles; taxonomy; trade; uncertainty; viruses; Asia; Australia; Europe; North America; South America
Abstract:
... Viruses in three genera of the family Iridoviridae (iridoviruses) affect finfish. Ranaviruses and megalocytiviruses are recently emerged pathogens. Both cause severe systemic disease, occur globally and affect a diversity of hosts. In contrast, lymphocystiviruses cause superficial lesions and rarely cause economic loss. The ranavirus epizootic haematopoietic necrosis virus (EHNV) from Australia wa ...
DOI:
10.1111/j.1365-2761.2009.01110.x
PubMed:
20050967

30. Genetic heterogeneity of betanodaviruses in juvenile production trials of Pacific bluefin tuna, Thunnus orientalis (Temminck '' Schlegel)

Author:
Sugaya, T.; Mori, K.; Nishioka, T.; Masuma, S.; Oka, M.; Mushiake, K.; Okinaka, Y.; Nakai, T.
Source:
Journal of fish diseases 2009 v.32 no.10 pp. 815-824
ISSN:
0140-7775
Subject:
Thunnus orientalis; breeding stock; coat proteins; disease transmission; genes; genetic heterogeneity; haplotypes; hatcheries; juveniles; necrosis; nucleotide sequences; population growth; reverse transcriptase polymerase chain reaction; tuna; Japan
Abstract:
... Pacific bluefin tuna, Thunnus orientalis (Temminck '' Schlegel), is one of the most important commercially exploited fish species in the world, and juvenile production techniques have been developed for its culture and stock enhancement in Japan. However, recent juvenile production has often failed because of the occurrence of viral nervous necrosis caused by betanodaviruses. In this study, we exa ...
DOI:
10.1111/j.1365-2761.2009.01057.x
PubMed:
19538459

31. Outbreak of betanodavirus infection in tilapia, Oreochromis niloticus (L.), in fresh water

Author:
Bigarré, L.; Cabon, J.; Baud, M.; Heimann, M.; Body, A.; Lieffrig, F.; Castric, J.
Source:
Journal of fish diseases 2009 v.32 no.8 pp. 667-673
ISSN:
0140-7775
Subject:
Oreochromis niloticus; antibodies; coat proteins; fluorescence; freshwater; genes; histopathology; larvae; mortality; nervous system; phylogeny; polymerase chain reaction; rearing
Abstract:
... A betanodavirus associated with a massive mortality was isolated from larvae of tilapia, Oreochromis niloticus, maintained in fresh water at 30 °C. Histopathology revealed vacuolation of the nervous system, suggesting an infection by a betanodavirus. The virus was identified by indirect fluorescent antibody test in the SSN1 cell line and further characterized by sequencing of a PCR product. Sequen ...
DOI:
10.1111/j.1365-2761.2009.01037.x
PubMed:
19500206

32. PCR amplification and sequence analysis of the major capsid protein gene of megalocytiviruses isolated in Taiwan

Author:
Wang, C.S.; Chao, S.Y.; Ku, C.C.; Wen, C.M.; Shih, H.H.
Source:
Journal of fish diseases 2009 v.32 no.6 pp. 543-550
ISSN:
0140-7775
Subject:
Epinephelus; Lates calcarifer; Leiognathus; Megalocytivirus; Rhabdosargus sarba; amino acids; coat proteins; freshwater fish; genes; genotype; grouper; marine fish; mortality; nucleotides; open reading frames; perch; phylogeny; polymerase chain reaction; sequence analysis; structural proteins; viruses; Taiwan
Abstract:
... Viruses belonging to the genus Megalocytivirus in the family Iridoviridae are one of the major agents causing mass mortalities in marine and freshwater fish in Asian countries. Outbreaks of iridovirus disease have been reported among various fish species in Taiwan. However, the genotypes of these iridoviruses have not yet been determined. In this study, seven megalocytivirus isolates from four fis ...
DOI:
10.1111/j.1365-2761.2009.01043.x
PubMed:
19460084

33. Emergence of pathogenic betanodaviruses belonging to the SJNNV genogroup in farmed fish species from the Iberian Peninsula

Author:
Cutrín, J.M.; Dopazo, C.P.; Thiéry, R.; Leao, P.; Olveira, J.G.; Barja, J.L.; Bandín, I.
Source:
Journal of fish diseases 2007 v.30 no.4 pp. 225-232
ISSN:
0140-7775
Subject:
Betanodavirus; Solea senegalensis; Sparus aurata; bass; coat proteins; databases; encephalopathy; farmed fish; fish culture; genes; genotype; industry; marine fish; necrosis; nucleotide sequences; phylogeny; retinal diseases; sequence analysis; Iberian Peninsula; Portugal; Spain
Abstract:
... Betanodaviruses are the causative agents of viral nervous necrosis (VNN) or viral encephalopathy and retinopathy (VER) in cultured marine fish. Based on the RNA2 gene fish nodaviruses have been traditionally classified into four different genotypes and recently a fifth genotype has been proposed. This study presents sequencing data of 24 new nodaviruses obtained from three different fish species: ...
DOI:
10.1111/j.1365-2761.2007.00803.x
PubMed:
17394524

34. Expression and serological application of a capsid protein of an iridovirus isolated from rock bream, Oplegnathus fasciatus (Temminck & Schlegel)

Author:
Kim, T.J.; Jung, T.S.; Lee, J.I.
Source:
Journal of fish diseases 2007 v.30 no.11 pp. 691-699
ISSN:
0140-7775
Subject:
Escherichia coli; Oplegnathus fasciatus; Ranavirus; antigens; bream; coat proteins; enzyme-linked immunosorbent assay; farmed fish; immune response; mice; morbidity; mortality; polyclonal antibodies
Abstract:
... Iridoviruses infect a wide variety of wild and cultured fish. Those iridoviruses belonging to the genus Ranavirus, in the Iridoviridae family, cause systemic disease in infected animals with a high morbidity and mortality. This paper reports the cloning, sequencing, and expression of the rock bream iridovirus (RBIV) major capsid protein (MCP) in an Escherichia coli expression system for subsequent ...
DOI:
10.1111/j.1365-2761.2007.00856.x
PubMed:
17958613

35. Identification of B-cell epitopes on the betanodavirus capsid protein

Author:
Costa, J.Z.; Adams, A.; Bron, J.E.; Thompson, K.D.; Starkey, W.G.; Richards, R.H.
Source:
Journal of fish diseases 2007 v.30 no.7 pp. 419-426
ISSN:
0140-7775
Subject:
B-lymphocytes; Dicentrarchus labrax; algorithms; amino acids; bass; blood serum; coat proteins; epitopes; mice; monoclonal antibodies; neutralization; prediction; synthetic peptides; vaccines
Abstract:
... The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted ...
DOI:
10.1111/j.1365-2761.2007.00824.x
PubMed:
17584439

36. Partial susceptibility of the SSN-1 fish cell line to a crustacean virus: a defective replication study

Author:
Hernandez-Herrera, R.I.; Chappe-Bonnichon, V.; Roch, P.; Sri Widada, J.; Bonami, J-R.
Source:
Journal of fish diseases 2007 v.30 no.11 pp. 673-679
ISSN:
0140-7775
Subject:
Macrobrachium rosenbergii; coat proteins; cytopathogenicity; electron microscopy; enzyme-linked immunosorbent assay; fish; genes; post-translational modification; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; virion
Abstract:
... This study evaluated the possible use of the fish SSN-1 cell line to investigate the development of Macrobrachium rosenbergii nodavirus (MrNV). Cells were incubated with viral particles and cytopathic effects were observed. De novo synthesis of viral capsid proteins was shown by immuno-fluorescence labelling and a sandwich ELISA test. Viral genomic replication was demonstrated by RT-PCR using prim ...
DOI:
10.1111/j.1365-2761.2007.00852.x
PubMed:
17958611

37. Involvement of serine proteinase in infectious pancreatic necrosis virus capsid protein maturation and NS proteinase cleavage in CHSE-214 cells

Author:
Wu, J.L.; Hong, J.R.; Chang, C.Y.; Hui, C.F.; Liao, C.F.; Hsu, Y.L.
Source:
Journal of fish diseases 1998 v.21 no.3 pp. 215-220
ISSN:
0140-7775
Subject:
Infectious pancreatic necrosis virus; Oncorhynchus tshawytscha; cell cleavage; coat proteins; proteinase inhibitors; serine proteinases
Abstract:
... An investigation of virus-specific protein maturation in infectious pancreatic necrosis virus (IPNV) infected Chinook salmon embryo cells (CHSE-214) was undertaken. The precursor protein (pVP2-1) of the major mature capsid protein (VP2) was processed sequentially from pVP2-1 to pVP2-2 and VP2. Experiments using serine proteinase inhibitors showed that the maturation of the VP2 was blocked in the p ...
DOI:
10.1046/j.1365-2761.1998.00097.x
PubMed:
21361976