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You searched for: Journal Journal of microbiological methods Remove constraint Journal: Journal of microbiological methods Publication Year 2018 Remove constraint Publication Year: 2018 Subject DNA Remove constraint Subject: DNA Subject term rapid methods Remove constraint Subject term: rapid methods
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1. Detection of Tilletia caries, Tilletia laevis and Tilletia controversa wheat grain contamination using loop-mediated isothermal DNA amplification (LAMP)

Author:
Katarzyna Pieczul; Agnieszka Perek; Krzysztof Kubiak
Source:
Journal of microbiological methods 2018 v.154 pp. 141-146
ISSN:
0167-7012
Subject:
Alternaria; Cladosporium; DNA; Fusarium; Helminthosporium; Penicillium; Tilletia controversa; Tilletia laevis; Tilletia tritici; detection limit; fungi; protocols; rapid methods; smut diseases; teliospores; wheat
Abstract:
... The study describes a novel diagnostic protocol based on a loop-mediated isothermal DNA amplification (LAMP) for identification of wheat grains infection by Tilletia laevis, Tilletia caries (common bunt) and Tilletia controversa (draft bunt). The presented data showed that the LAMP analysis is a simple, specific and rapid method that could be used for detection of Tilletia spp. in contaminated gra ...
DOI:
10.1016/j.mimet.2018.10.018

2. Development of a tick-borne pathogen QPCR panel for detection of Anaplasma, Ehrlichia, Rickettsia, and Lyme disease Borrelia in animals

Author:
Zhenyu Shen; Michael Z. Zhang; Roger W. Stich; William J. Mitchell; Shuping Zhang
Source:
Journal of microbiological methods 2018 v.151 pp. 83-89
ISSN:
0167-7012
Subject:
Anaplasma; Borrelia; DNA; Ehrlichia; Lyme disease; Rickettsia; blood; cross reaction; dogs; horses; humans; melting point; pathogens; rapid methods; screening
Abstract:
... Anaplasma spp., Ehrlichia spp., Rickettsia spp., and Lyme disease associated Borrelia spp. are the most common tick-borne pathogens reported to infect human beings worldwide and other animals, such as dogs and horses. In the present study, we developed a broad-coverage SYBR Green QPCR panel consisting of four individual assays for the detection and partial differentiation of the aforementioned pat ...
DOI:
10.1016/j.mimet.2018.05.019

3. Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay

Author:
Leonardo Alves Rusak; Rodrigo de Castro Lisboa Pereira; Isabelle Geoffroy Freitag; Cristina Barroso Hofer; Ernesto Hofer; Marise Dutra Asensi; Deyse Christina Vallim
Source:
Journal of microbiological methods 2018 v.154 pp. 107-111
ISSN:
0167-7012
Subject:
DNA; Yersinia enterocolitica; biosynthesis; digestive system diseases; feces; hospitals; humans; monitoring; peptide elongation factors; polymerase chain reaction; pregnant women; public health; rapid methods; selective media; serotypes; Europe
Abstract:
... Yersinia enterocolitica, a member of the Enterobacteriaceae family, is a zoonotic agent that causes gastrointestinal diseases and some extraintestinal disorders in humans. Y. enterocolitica ssp. palearctica bioserotype 4/O:3 is the primary pathogenic bioserotype in Europe, where it has a high public health relevance. The isolation and identification of Y. enterocolitica from various sources on sel ...
DOI:
10.1016/j.mimet.2018.10.014

4. Sensitive and rapid detection of Salmonella enterica serovar Indiana by cross-priming amplification

Author:
Yong-xiang Wang; An-yun Zhang; Yong-qiang Yang; Chang-wei Lei; Guang-yang Cheng; Wen-cheng Zou; Jin-xin Zeng; Yan-peng Chen; Hong-ning Wang
Source:
Journal of microbiological methods 2018 v.153 pp. 24-30
ISSN:
0167-7012
Subject:
DNA; Salmonella Indiana; bacteria; detection limit; food contamination; food pathogens; foods; host range; humans; nucleotide sequences; poultry; quantitative polymerase chain reaction; rapid methods; screening; swine
Abstract:
... Salmonella enterica serovar Indiana (S. Indiana) was the most frequently reported foodborne pathogen, which has a broad host range including poultry, swine, and humans. Traditional methods used for the detection of S. Indiana from contaminated food products are time-consuming and labor-intensive. Therefore, rapid detection methods with high sensitivity and specificity are vitally important to prev ...
DOI:
10.1016/j.mimet.2018.08.003

5. A real-time loop-mediated isothermal amplification method for rapid detection of Lawsonia intracellularis in porcine fecal samples

Author:
Yanan Li; Jianchang Wang; Jinfeng Wang; Libing Liu; Ruoxi Zhang; Ruihan Shi; Qingan Han; Jiguo Sun; Wanzhe Yuan
Source:
Journal of microbiological methods 2018 v.151 pp. 62-65
ISSN:
0167-7012
Subject:
DNA; Lawsonia intracellularis; diarrhea; feces; genes; intestinal mucosa; loop-mediated isothermal amplification; pathogens; point-of-care systems; porcine proliferative enteritis; quantitative polymerase chain reaction; rapid methods; ribosomal RNA; swine; turbidity
Abstract:
... Porcine proliferative enteritis is a common diarrheal disease characterized by thickening of the intestinal mucosa in swine due to enterocyte proliferation, which is caused by Lawsonia intracellularis. In this study, a real-time loop-mediated isothermal amplification (LAMP) assay was developed to detect L. intracellularis based on the conserved region of the 16S ribosomal RNA gene. The optimal rea ...
DOI:
10.1016/j.mimet.2018.06.002