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You searched for: Journal Journal of microbiological methods Remove constraint Journal: Journal of microbiological methods Subject term Lactobacillus Remove constraint Subject term: Lactobacillus
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1. Baby-SPIME: A dynamic in vitro piglet model mimicking gut microbiota during the weaning process

Author:
Sandrine Dufourny; Nadia Everaert; Sarah Lebrun; Caroline Douny; Marie-Louise Scippo; Bing Li; Bernard Taminiau; Massimo Marzorati; José Wavreille; Eric Froidmont; Pierre Rondia; Véronique Delcenserie
Source:
Journal of microbiological methods 2019 v.167 pp. 105735
ISSN:
0167-7012
Subject:
Bacteroides; Bifidobacterium; Fusobacterium; Lactobacillus; Megasphaera; Mitsuokella; Prevotella; acetates; animal models; bioreactors; butyric acid; colon; delta-Proteobacteria; fermentation; gamma-Proteobacteria; humans; ileum; in vitro digestion; intestinal microorganisms; metabolites; microbial communities; piglets; propionic acid; weaning
Abstract:
... The study aimed to adapt the SHIME® model, developed to simulate human digestion and fermentation, to a baby-SPIME (baby Simulator of Pig Intestinal Microbial Ecosystem). What constitutes a unique feature of this model is its twofold objective of introducing an ileal microbial community and mimicking a dietary weaning transition. This model should then be ideally suited to test the dietary weaning ...
DOI:
10.1016/j.mimet.2019.105735

2. Characterization of growth inhibition of oral bacteria by sophorolipid using a microplate-format assay

Author:
Daniel K.Y. Solaiman; Richard D. Ashby; Joseph Uknalis
Source:
Journal of microbiological methods 2017 v.136 pp. 21-29
ISSN:
0167-7012
Subject:
Lactobacillus; Streptococcus mutans; absorbance; adverse effects; antibacterial properties; bacteria; biosurfactants; cell growth; glycolipids; growth retardation; hygiene; microbial growth; scanning electron microscopy; yeasts
Abstract:
... Sophorolipid (SL) is a class of glycolipid biosurfactant produced by yeast and has potent antimicrobial activity against many microorganisms. In this paper, a microplate-based method was developed to characterize the growth inhibition by SL on five representative species of caries-causing oral bacteria. Bacterial growth on microplate in the absence and presence of varying concentrations of SL was ...
DOI:
10.1016/j.mimet.2017.02.012

3. Comparison of procedures for the extraction of supernatants and cytotoxicity tests in Vero cells, applied to assess the toxigenic potential of Bacillus spp. and Lactobacillus spp., intended for use as probiotic strains

Author:
Anicet R. Blanch; Javier Méndez; Susana Castel; Manuel Reina
Source:
Journal of microbiological methods 2014 v.103 pp. 64-69
ISSN:
0167-7012
Subject:
Bacillus cereus; Bacillus toyonensis; Lactobacillus; ammonium sulfate; animal nutrition; cytotoxicity; feeds; humans; methanol; probiotics; protein synthesis; protocols; ultrafiltration
Abstract:
... Interest in using Bacillus strains as probiotic components of animal feeds has grown in recent years. However, some of these strains, especially those taxonomically related to the Bacillus cereus group, may have enterotoxigenic activity. Assessment of their toxigenic potential by well-established and robust protocols is required before authorizing their use in animal nutrition. Three methods of ex ...
DOI:
10.1016/j.mimet.2014.05.019

4. Development of a Lactobacillus specific T-RFLP method to determine lactobacilli diversity in complex samples

Author:
Long Chen; Matt T. Teasdale; Melissa M. Kaczmarczyk; Gregory G. Freund; Michael J. Miller
Source:
Journal of microbiological methods 2012 v.91 no.2 pp. 262-268
ISSN:
0167-7012
Subject:
Lactobacillus; databases; dietary supplements; digestion; feces; mice; microbial communities; principal component analysis; restriction endonucleases; restriction fragment length polymorphism; ribosomal DNA
Abstract:
... Terminal restriction fragment length polymorphism (T-RFLP) analysis has been widely used for studying microbial communities. However, most T-RFLP assays use 16S rDNA as the target and are unable to accurately characterize a microbial subpopulation. In this study, we developed a novel T-RFLP protocol based on Lactobacillus hsp60 to rapidly characterize and compare lactobacilli composition. The theo ...
DOI:
10.1016/j.mimet.2012.08.005
PubMed:
22981747
PubMed Central:
PMC3478482
CHORUS:
10.1016/j.mimet.2012.08.005

5. Establishment of a sensitive system for analysis of human vaginal microbiota on the basis of rRNA-targeted reverse transcription-quantitative PCR

Author:
Takashi Kurakawa; Kiyohito Ogata; Hirokazu Tsuji; Yukiko Kado; Takuya Takahashi; Yumi Kida; Masahiro Ito; Nobuhiko Okada; Koji Nomoto
Source:
Journal of microbiological methods 2015 v.111 pp. 93-104
ISSN:
0167-7012
Subject:
Atopobium vaginae; Bifidobacterium adolescentis; Bifidobacterium angulatum; Bifidobacterium longum subsp. infantis; Chlamydia trachomatis; DNA primers; Gardnerella vaginalis; Lactobacillus crispatus; Lactobacillus gasseri; Lactobacillus jensenii; Lactobacillus vaginalis; Mobiluncus curtisii; Prevotella; Streptococcus; anaerobes; bacteria; humans; patients; quantitative analysis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:
... Ten specific primer sets, for Lactobacillus gasseri, Lactobacillus crispatus, Atopobium vaginae, Gardnerella vaginalis, Mobiluncus curtisii, Chlamydia trachomatis/muridarum, Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium adolescentis, and Bifidobacterium angulatum, were developed for quantitative analysis of vaginal microbiota. rRNA-targeted reverse t ...
DOI:
10.1016/j.mimet.2015.01.021

6. Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection

Author:
L.H.B. Hansen; B. Nielsen; E.J. Boll; L. Skjøt-Rasmussen; A. Wellejus; L. Jørgensen; C. Lauridsen; N. Canibe
Source:
Journal of microbiological methods 2021 v.180 pp. 106126
ISSN:
0167-7012
Subject:
Bacillus subtilis; Bifidobacterium animalis subsp. lactis; Enterococcus faecium; Escherichia infections; Lactobacillus; agar; diarrhea; enterotoxigenic Escherichia coli; epithelium; human cell lines; intestines; lactic acid; mechanism of action; milk; oligosaccharides; permeability; probiotics; protective effect; sodium; swine production
Abstract:
... Enterotoxigenic Escherichia coli (ETEC), being the major cause of post-weaning diarrhoea (PWD) in newly weaned piglets, induces poor performance and economic losses in pig production. This functional in vitro screening study investigated probiotic strains for use in suckling piglets as a prophylactic strategy towards PWD. Nine strains were evaluated based on their ability to: enhance intestinal ep ...
DOI:
10.1016/j.mimet.2020.106126

7. High-throughput analysis of the impact of antibiotics on the human intestinal microbiota composition

Author:
S.E. Ladirat; H.A. Schols; A. Nauta; M.H.C. Schoterman; B.J.F. Keijser; R.C. Montijn; H. Gruppen; F.H.J. Schuren
Source:
Journal of microbiological methods 2013 v.92 no.3 pp. 387-397
ISSN:
0167-7012
Subject:
Bacteroides; Bifidobacterium; Clostridium; Enterobacteriaceae; Lactobacillus; adults; antibiotics; bacteria; dose response; fermentation; humans; inoculum; intestinal microorganisms; microarray technology; phylogeny; screening
Abstract:
... Antibiotic treatments can lead to a disruption of the human microbiota. In this in-vitro study, the impact of antibiotics on adult intestinal microbiota was monitored in a new high-throughput approach: a fermentation screening-platform was coupled with a phylogenetic microarray analysis (Intestinal-chip). Fecal inoculum from healthy adults was exposed in a fermentation screening-platform to seven ...
DOI:
10.1016/j.mimet.2012.12.011

8. High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species

Author:
Raffael C. Inglin; Marc J.A. Stevens; Lukas Meile; Christophe Lacroix; Leo Meile
Source:
Journal of microbiological methods 2015 v.114 pp. 26-29
ISSN:
0167-7012
Subject:
Lactobacillus; antifungal properties; laboratory equipment; screening
Abstract:
... We describe high-throughput screening techniques to rapidly detect either antimicrobial activity, using an agar-well diffusion assay in microtiter plates, or antifungal activity using an agar-spot assay in 24-well plates. 504 Lactobacillus isolates were screened with minimal laboratory equipment and screening rates of 2000–5000 individual antimicrobial interactions. ...
DOI:
10.1016/j.mimet.2015.04.011

9. Kinetic analysis of microcalorimetric data derived from microbial growth: Basic theoretical, practical and industrial considerations

Author:
Miroslav Cabadaj; Shazia Bashir; David Haskins; Jawal Said; Laura McCoubrey; Simon Gaisford; Anthony Beezer
Source:
Journal of microbiological methods 2021 v.187 pp. 106276
ISSN:
0167-7012
Subject:
bacterial growth; calorimetry; kinetics; probiotics; product quality
Abstract:
... We report here a mathematical framework for the quantitative interpretation of exponential bacterial growth measured with isothermal microcalorimetry. The method allows determination of many parameters that define the exponential growth phase. To automate the analysis, we also wrote a coding program, so that the approach could be embedded in a commercial setting. As an exemplar, we apply the metho ...
DOI:
10.1016/j.mimet.2021.106276

10. Metagenomics approach to the study of the gut microbiome structure and function in zebrafish Danio rerio fed with gluten formulated diet

Author:
Hyunmin Koo; Joseph A. Hakim; Mickie L. Powell; Ranjit Kumar; Peter G. Eipers; Casey D. Morrow; Michael Crowley; Elliot J. Lefkowitz; Stephen A. Watts; Asim K. Bej
Source:
Journal of microbiological methods 2017 v.135 pp. 69-76
ISSN:
0167-7012
Subject:
Bacteroides; Danio rerio; Lactobacillus; Legionellales; Rhizobiaceae; Rhodobacter; adverse effects; animal models; bacteria; bile acids; bile secretion; bioinformatics; biosynthesis; diet; digestive system; digestive system diseases; ecosystems; gluten; intestinal microorganisms; metagenomics; microbial communities; microbiome; serine; threonine; vitamin B12
Abstract:
... In this study, we report the gut microbial composition and predictive functional profiles of zebrafish, Danio rerio, fed with a control formulated diet (CFD), and a gluten formulated diet (GFD) using a metagenomics approach and bioinformatics tools. The microbial communities of the GFD-fed D. rerio displayed heightened abundances of Legionellales, Rhizobiaceae, and Rhodobacter, as compared to the ...
DOI:
10.1016/j.mimet.2017.01.016
PubMed:
28167213
PubMed Central:
PMC5909692

11. Multi fragment melting analysis system (MFMAS) for one-step identification of lactobacilli

Author:
Zülal Kesmen; Özge Kılıç; Yasin Gormez; Mete Çelik; Burcu Bakir-Gungor
Source:
Journal of microbiological methods 2020 v.177 pp. 106045
ISSN:
0167-7012
Subject:
DNA; Lactobacillus; computer software; fermentation; probiotics
Abstract:
... The accurate identification of lactobacilli is essential for the effective management of industrial practices associated with lactobacilli strains, such as the production of fermented foods or probiotic supplements. For this reason, in this study, we proposed the Multi Fragment Melting Analysis System (MFMAS)-lactobacilli based on high resolution melting (HRM) analysis of multiple DNA regions that ...
DOI:
10.1016/j.mimet.2020.106045

12. Novel microaerobic agar plate method delivers highly selective and accurate enumeration of probiotic lactobacilli in freeze-dried blends containing bifidobacteria

Author:
Phillip M. Byrd; Vincenzo Fallico; Peipei Tang; Connie Wong
Source:
Journal of microbiological methods 2022 v.195 pp. 106451
ISSN:
0167-7012
Subject:
Bifidobacterium; Lactobacillus; agar; antibiotics; carbon dioxide; dietary supplements; food industry; freeze drying; oxygen; probiotics
Abstract:
... The enumeration of viable bacteria is an essential metric in the dietary supplement and food industry to ensure quality of probiotic products. However, selective enumeration of lactobacilli in probiotic freeze-dried blends containing bifidobacteria is difficult to achieve with current Lactobacillus-specific agars (i.e., Rogosa and LAMVAB). Using a panel of Bifidobacterium and Lactobacillus commerc ...
DOI:
10.1016/j.mimet.2022.106451

13. Rapid differentiation of Lactobacillus species via metabolic profiling

Author:
Kundi Yang; Mengyang Xu; Fanyi Zhong; Jiangjiang Zhu
Source:
Journal of microbiological methods 2018 v.154 pp. 147-155
ISSN:
0167-7012
Subject:
Lactobacillus; data quality; digestive system; functional properties; humans; lactic acid; lactic acid bacteria; metabolites; metabolomics; monitoring; probiotics; spectrometers; sugars
Abstract:
... Lactobacillus, the major genus of lactic acid bacteria group, plays functional roles in the human body, for example, convert sugars to lactic acid. They are the significant microbiota which can be found at a number of human body sites, such as the digestive system, urinary system, and genital system. A number of Lactobacillus species are often used as probiotics and can benefit host health when ad ...
DOI:
10.1016/j.mimet.2018.10.013

14. Silkworm larvae plasma (SLP) assay for detection of bacteria: False positives secondary to inflammation in vivo

Author:
Michelle Ma; Tyler A. Rice; Caroline M. Percopo; Helene F. Rosenberg
Source:
Journal of microbiological methods 2017 v.132 pp. 9-13
ISSN:
0167-7012
Subject:
bacteria; inflammation; insect larvae; melanin; microbial detection; peptidoglycans
Abstract:
... The silkworm larvae plasma (SLP) assay has been developed as a means to detect bacterial peptidoglycan as a surrogate for live bacteria. Here, we present results that indicate that generation of melanin by this assay is not fully reliable as a surrogate marker for bacterial count. ...
DOI:
10.1016/j.mimet.2016.11.009
PubMed:
27840194
PubMed Central:
PMC5195888

15. Spontaneous bacteriocin resistance in Listeria monocytogenes as a susceptibility screen for identifying different mechanisms of resistance and modes of action by bacteriocins of lactic acid bacteria

Author:
Sunita Macwana; Peter M. Muriana
Source:
Journal of microbiological methods 2012 v.88 no.1 pp. 7-13
ISSN:
0167-7012
Subject:
Lactobacillus; Lactococcus lactis; Listeria monocytogenes; Pediococcus; bacteriocins; biopreservatives; cross resistance; lactic acid bacteria; mechanism of action; mutation; screening
Abstract:
... A practical system was devised for grouping bacteriocins of lactic acid bacteria (LAB) based on mode of action as determined by changes in inhibitory activity to spontaneously-acquired bacteriocin resistance (Bacᴿ). Wild type Listeria monocytogenes 39–2 was sensitive to five bacteriocins produced by 3 genera of LAB: pediocin PA-1 and pediocin Bac3 (Pediococcus), lacticin FS97 and lacticin FS56 (La ...
DOI:
10.1016/j.mimet.2011.09.009

16. Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) production and Lactobacillus species growth in a defined medium simulating vaginal secretions

Author:
Robin L. Stingley; Huanli Liu; Lisa B. Mullis; Christopher A. Elkins; Mark E. Hart
Source:
Journal of microbiological methods 2014 v.106 pp. 57-66
ISSN:
0167-7012
Subject:
Lactobacillus; Staphylococcus aureus; albumins; amino acids; bacteria; glucose; glycogen; hydrogen peroxide; manganese chloride; mucins; pathogens; polyethylene glycol; secretion; septic shock; sodium acetate; sodium bicarbonate; urea; vitamins
Abstract:
... Lactobacillus species are commensal with the healthy vaginal environment and inhibit the growth of many pathogenic bacteria in the vaginal tract by a variety of mechanisms, such as the production of hydrogen peroxide, organic acids, and antimicrobial substances. Simulation of the vaginal environment is crucial for proper investigation of the effects of Lactobacillus species on pathogenic bacteria. ...
DOI:
10.1016/j.mimet.2014.08.002

17. An adaptable and non-invasive method for tracking Bifidobacterium animalis subspecies lactis 420 in the mouse gut

Author:
Marissa A. Lopez-Pier; Matthew P. Koppinger; Preston R. Harris; Danielle K. Cannon; Rinku S. Skaria; Bonnie L. Hurwitz; George Watts; Shravan Aras; Marvin J. Slepian; John P. Konhilas
Source:
Journal of microbiological methods 2021 v.189 pp. 106302
ISSN:
0167-7012
Subject:
Bifidobacterium animalis; Lactobacillus; cardiovascular diseases; digestive tract; intestinal microorganisms; intestines; mice; probiotics; quantitative polymerase chain reaction
Abstract:
... Probiotic strains from the Bifidobacterium or Lactobacillus genera improve health outcomes in models of metabolic and cardiovascular disease. Yet, underlying mechanisms governing these improved health outcomes are rooted in the interaction of gut microbiota, intestinal interface, and probiotic strain. Central to defining the underlying mechanisms governing these improved health outcomes is the dev ...
DOI:
10.1016/j.mimet.2021.106302

18. A dynamic regression analysis tool for quantitative assessment of bacterial growth written in Python

Author:
Jennifer L. Hoeflinger; Daniel E. Hoeflinger; Michael J. Miller
Source:
Journal of microbiological methods 2017 v.132 pp. 83-85
ISSN:
0167-7012
Subject:
Lactobacillus; microbial growth; regression analysis; specific growth rate
Abstract:
... Herein, an open-source method to generate quantitative bacterial growth data from high-throughput microplate assays is described. The bacterial lag time, maximum specific growth rate, doubling time and delta OD are reported. Our method was validated by carbohydrate utilization of lactobacilli, and visual inspection revealed 94% of regressions were deemed excellent. ...
DOI:
10.1016/j.mimet.2016.11.015

19. A kinetic microplate method for quantifying the antibacterial properties of biological fluids

Author:
Holowachuk, Scott A.; Bal'a, M. Farid; Buddington, Randal K.
Source:
Journal of microbiological methods 2003 v.55 no.2 pp. 441-446
Subject:
pancreatic juice; Staphylococcus aureus; Escherichia coli; Lactobacillus; microbial growth; bioassays
Abstract:
... The microplate assay for measuring antibacterial activity was adapted by incorporating a known concentration range of gentamicin as an internal standard. Staphylococcus aureus subsp. aureus strain ATCC 6538P, Escherichia coli ATCC 25922, and Lactobacillus spp. were used as target organisms, although other indicator organisms and antibiotics can be examined. Assay range and sensitivity were depende ...
Handle:
10113/48128
DOI:
10.1016/S0167-7012(03)00190-8

20. A novel image cytometry-based Lactobacillus bacterial enumeration method for the production of kettle sour beer

Author:
Matthew Hodgkin; Suzanne M. Purseglove; Leo Li-Ying Chan; Jennifer Perry; Jason Bolton
Source:
Journal of microbiological methods 2020 v.177 pp. 106031
ISSN:
0167-7012
Subject:
Lactobacillus; automation; beers; fermentation; plate count; quality control
Abstract:
... The demands for a variety of craft beer flavors have been increasing in the United States. To meet this rising demand, breweries have been experimenting with kettle sour beer that utilizes lactic acid-producing bacteria for fermentation. The current standard bacterial quantification method is insufficient for rigorous quality control, thus there is a need for a better method to monitor lactobacill ...
DOI:
10.1016/j.mimet.2020.106031

21. A novel pharmaceutical approach for the analytical validation of probiotic bacterial count by flow cytometry

Author:
Luca Michelutti; Michela Bulfoni; Emanuele Nencioni
Source:
Journal of microbiological methods 2020 v.170 pp. 105834
ISSN:
0167-7012
Subject:
Bifidobacterium; Lactobacillus; acidification; bacteria; biochemical pathways; cell viability; chemical species; computer software; data analysis; dietary supplements; drugs; fermentation; flow cytometry; fluorescent dyes; humans; oxygen; plate count; probiotics; propidium; thiazoles
Abstract:
... Introduction: Flow cytometry is a powerful and sensitive technique able to characterize single cells within a heterogeneous population. Different fluorescent dyes can be combined and used together to analyze a great variety of parameters simultaneously. In particular, flow-cytometry allows to measure viability and vitality of probiotics measuring their metabolic activity, fermentation capacity, ac ...
DOI:
10.1016/j.mimet.2020.105834

22. A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria

Author:
Lidia Hanna Markiewicz; Agata Szymkiewicz; Anna Szyc; Barbara Wróblewska
Source:
Journal of microbiological methods 2016 v.126 pp. 72-75
ISSN:
0167-7012
Subject:
Lactobacillus; Western blotting; antibodies; bacterial proteins; humans; immunoglobulin E; immunoglobulin G; lactic acid bacteria; patients
Abstract:
... Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE. ...
DOI:
10.1016/j.mimet.2016.05.007

23. A ‘bacteriocin PCR array’ for identification of bacteriocin-related structural genes in lactic acid bacteria

Author:
Sunita J. Macwana; Peter M. Muriana
Source:
Journal of microbiological methods 2012 v.88 no.2 pp. 197-204
ISSN:
0167-7012
Subject:
DNA; Lactobacillus; Lactococcus; Pediococcus; agarose; amino acid sequences; bacteriocins; food preservatives; gels; genetic databases; lactic acid bacteria; melting; nucleotide sequences; quantitative polymerase chain reaction; sequence homology; structural genes
Abstract:
... Bacteriocins have been identified in many strains of lactic acid bacteria (LAB) which are a source of natural food preservatives and microbial inhibitors. Our objectives were to use a PCR array of primers to identify bacteriocin structural genes in Bac⁺ LAB. DNA sequence homology at the 5′- and 3′-ends of the various structural genes indicated that non-specific priming may allow PCR amplification ...
DOI:
10.1016/j.mimet.2011.11.008