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... Mesorhizobium loti is a Gram negative bacterium that induces N₂-fixing root nodules on the model legume Lotus japonicus. Proteomic analysis in M. loti indicated that 3-phosphoglycerate dehydrogenase (EC. 1.1.1.95, PHGDH) protein content was 2.2 times higher in bacteroids than in cultured bacteria. A M. loti mutant (STM5) with a transposon insertion in the PHGDH gene, mll3875, showed an absolute de ...
... AIMS: This work was conducted to examine the effects of volatile organic compounds (VOCs) from Trichoderma virens and the 4-phosphopantetheinyl transferase 1 (TvPPT1) mutant in growth promotion and induction of defense responses of Arabidopsis thaliana seedlings using a co-cultivation system in vitro. METHODS: The contribution of VOCs to plant development and immunity was assessed by comparing the ...
Taraxacum officinale; abscisic acid; biosynthesis; corn; field experimentation; habitats; tungsten; water stress
Abstract:
... The effect of tungsten as a potential inhibitor of ABA biosynthesis in plants was tested both under controlled conditions in the greenhouse or laboratory and in the field at a site naturally enriched with tungsten. Although as predicted in all cases the activity of aldehyde oxidase was strongly inhibited by tungsten, in well watered, greenhouse-grown maize plants increased concentrations of tungst ...
... White lupin (Lupinus albus L.) acclimates to phosphorus deficiency (-P) by the development of short, densely clustered lateral roots called proteoid (or cluster) roots. These specialized plant organs display increased exudation of citric and malic acid. The enhanced exudation of organic acids from P stressed white lupin roots is accompanied by increased in vitro phosphoenolpyruvate carboxylase (PE ...
... BACKGROUND AND AIMS: Recent studies suggest that root border cells function in defense of the root by an extracellular DNA-based trapping mechanism similar to that described in mammalian white blood cells. Genes controlling the specialized properties of border cells as they detach from the root tip therefore are of interest. METHODS: mRNA differential display was used to identify Brd13, a sequence ...
... AIMS: Arabidopsis thaliana is the model plant that is mainly used in studying cellulose and hemicellulose (CH) biosynthesis. Unfortunately, A. thaliana does not associate with mycorrhiza and as a result there are only rare reports on the role of arbuscular mycorrhiza (AM) fungi on CH biosynthesis. This study aims to investigate the effects of AM fungi on changing the CH content in mycorrhizal plan ...
... PURPOSE: Numerous Bacillus spp. in soils show strong plant growth-promotion effects, but the molecular mechanism of most of these bacteria interacting with plants remains unknown. Uncovering the interaction pattern is of considerable importance for potential applications in agriculture. METHODS: In the present study, we isolated the bacterium Bacillus megaterium strain WW1211 from the rhizosphere ...
... PURPOSE: Iron (Fe) deficiency seriously affects crop growth and yield. This study investigated whether and how an endophytic isolate, strain Bacillus sp. WR12, alleviates Fe deficiency in wheat (Triticum aestivum L.). METHODS: Bacillus sp. WR12 and wheat seedlings were hydroponically co-cultured for 2 weeks under Fe-deficient conditions. To evaluate the Fe deficiency alleviating potential, wheat g ...
... Ionic stress caused by high aluminum (Al) concentrations is one of the most widespread phytotoxicity problems globally in agricultural regions, greatly limiting crop yield in affected areas. The objective of this work was to examine a possible involvement of boron (B) in the detoxification of Al by stimulating glutathione (GSH) metabolism, a mechanism essential for the resistance of plants under s ...
Populus nigra; air; biosynthesis; carbon dioxide; chemical degradation; coppicing; dormancy; free air carbon dioxide enrichment; growing season; lignin; nitrogen; nitrogen content; nitrogen fertilizers; nutrient use efficiency; phenolic compounds; proteins; seasonal variation; secondary metabolites; wood; wood chemistry
Abstract:
... The goal of this study was to investigate whether increased nitrogen use efficiency found in Populus nigra L. (Jean Pourtet) under elevated CO₂ would correlate with changes in the production of carbon-based secondary compounds (CBSCs). Using Free-Air CO₂ Enrichment (FACE) technology, a poplar plantation was exposed to either ambient (about 370 μmol mol-¹ CO₂) or elevated (about 550 μmol mol-¹ CO₂) ...
Phytolacca acinosa; X-ray absorption spectroscopy; biosynthesis; centrifugation; chemical analysis; chemical speciation; gel chromatography; hydroponics; hyperaccumulators; leaves; manganese; oxalates; oxalic acid
Abstract:
... Phytolacca acinosa Roxb. is a Mn hyperaccumulating plant. In the present study, the chemical forms of Mn in the leaves of P. acinosa were investigated using chemical analyses and X-ray absorption spectroscopy (XAS). P. acinosa plants were grown hydroponically with 2 mM Mn for 28 days. About 80% of the Mn in the leaves of P. acinosa was found in the supernatant fraction after centrifugation at 20,0 ...
... AIMS: Abscisic acid (ABA) plays an important role in the stress tolerance of seedlings and 9-cis-epoxycarotenoid dioxygenase (NCED) is considered to be the rate-limiting enzyme involved in ABA biosynthesis. However, the genes encoding NCED in M. hupehensis Rehd. have not been reported. METHODS: In this study, a gene encoding NCED, MhNCED3, was isolated from the roots of M. hupehensis Rehd. Its fun ...
Mariana Ramos Leandro; Patrícia Louzada Rangel; Tamires Cruz dos Santos; Leandro Fernandes Andrade; Luciano de Souza Vespoli; Ana Lidia Soares Rangel; Suzane Ariadina de Souza; Roberta Ribeiro Barbosa; Lucas Zanchetta Passamani; Vanildo Silveira; Gonçalo Apolinário de Souza Filho
... BACKGROUND AND AIMS: Herbaspirillum seropedicae is a Plant Growth Promoting Bacterium (PGPB) that colonizes crops of economic interest, especially grasses. Here, we aimed to characterize the association of H. seropedicae with the model dicot Arabidopsis thaliana and its effect on plant growth. The molecular pathways activated during the association were monitored by comparative proteomics. METHODS ...
... AIMS: Clubroot, caused by the soil-borne protist Plasmodiophora brassicae, is one of the most destructive disease for Brassica oleracea worldwide. However, the molecular mechanism of clubroot resistance still remains poorly elucidated. Therefore, we aim at identifying key genes responsive to P. brassicae infection and deducing possible molecular mechanism regulating clubroot resistance in cabbage. ...
... BACKGROUND: Extracellular polymeric matrix (EPM) is a complex component of the organo-mineral assemblages created by biological soil crusts (BSCs). Mainly of polysaccharidic origin, it embeds soil and sediments and provides key benefits to the crust community. Services provided include: sediment cohesion and resistance to erosion, moisture provision, protection from external harmful factors, as we ...
... BACKGROUND: Net rates of biosynthesis and mineral deposition are needed to understand the physiology and molecular biology of growth and plant responses to environmental variation. Many popular models ignore cell expansion and displacement. In contrast, the continuity equation, used with empirical data on growth velocity and concentration, allows computation of biosynthesis and deposition rates in ...
... AIMS: Accumulation of defense metabolites is an adaptive response to heavy metals in plants. However, deployment of multiple classes of defense metabolites under cadmium (Cd) and copper (Cu) toxicity is not understood. Here, the responses of parthenolide and phenylpropanoids were examined. METHODS: Quantification of parthenolide and phenylpropanoids, and the expression of parthenolide biosynthesis ...
... AIMS: Oilseed rape (Brassica napus L.) has high nitrogen (N) requirement for its optimal growth and yield. However, little is known about the transcriptomic reprogramming of rapeseed in response to N deficiency. METHODS: Two rapeseed genotypes with contrasting N use efficiency (NUE) were used to perform whole-transcriptome sequencing on the shoots and roots supplied with or without N in a time-cou ...