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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 1997 Remove constraint Publication Year: 1997 Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum
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1. Antisense expression and overexpression of biotin carboxylase in tobacco leaves

Author:
Shintani, D.; Roesler, K.; Shorrosh, B.; Savage, L.; Ohlrogge, J.
Source:
Plant physiology 1997 v.114 no.3 pp. 881-886
ISSN:
0032-0889
Subject:
1-aminocyclopropane-1-carboxylic acid; Nicotiana tabacum; leaves; plastids; fatty acids; biosynthesis; carboxy-lyases; biotin; enzyme activity; protein composition; quantitative analysis; genetic transformation; transgenic plants; antisense DNA; gene expression; genetic variation
Abstract:
... The plastid acetyl-coenzyme A carboxylase (ACCase) catalyzes the first committed step of fatty acid synthesis and in most plants is present as a heteromeric complex of at least four different protein subunits: the biotin carboxylase (BC), the biotin carboxyl carrier protein, and the alpha and beta subunits of the carboxyltransferase. To gain insight into the subunit organization of this heteromeri ...
PubMed:
9232874
PubMed Central:
PMC158375

2. Association of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissues in several dicot plants

Author:
Ye, Z.H.
Source:
Plant physiology 1997 v.115 no.4 pp. 1341-1350
ISSN:
0032-0889
Subject:
developmental stages; lignification; roots; stems; methylation; immunohistochemistry; gene expression; Nicotiana tabacum; protein composition; plant development; Glycine max; Zinnia violacea; phloem; xylem; lignin; Solanum lycopersicum var. lycopersicum; biosynthesis; caffeic acid; Medicago sativa; Forsythia suspensa; coenzyme A; transferases
Abstract:
... Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) was previously shown to be associated with lignificationin both in vitro tracheary elements (TEs) and organs of zinnia (Zinnia elegans). However, it is not known whether this is a general pattern in dicot plants. To address this question, polyclonal antibodies against zinnia recombinant CCoAOMT fusion protein were raised and used for immunolocali ...
PubMed:
9414548
PubMed Central:
PMC158599

3. Auxin-induced epinasty of tobacco leaf tissues. A nonethylene-mediated response

Author:
Keller, C.P.; Van Volkenburgh, E.
Source:
Plant physiology 1997 v.113 no.2 pp. 603-610
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell growth; epinasty; dose response; acetic acid; ethylene; biosynthesis; naphthaleneacetic acid; aminooxyacetic acid; indole acetic acid
Abstract:
... Interveinal strips (10 x 1.5 mm) excised from growing tobacco (Nicotiana tabacum L. cv Xanthi) leaves curled >300 degrees when incubated for 20 h in 5 to 500 micromolar alpha-naphthalene acetic acid or 50 to 500 micromolar indole-3-acetic acid. Epinasty was not induced without auxin or by the auxin analog beta-naphthalene acetic acid, and less substantial epinasty was induced in midrib and vein se ...

4. Changes in the activity and mRNA of cinnamyl alcohol dehydrogenase during tracheary element differentiation in zinnia

Author:
Sato, Y.; Watanabe, T.; Komamine, A.; Hibino, T.; Shibata, D.; Sugiyama, M.; Fukuda, H.
Source:
Plant physiology 1997 v.113 no.2 pp. 425-430
ISSN:
0032-0889
Subject:
Zinnia violacea; mesophyll; lignin; biosynthesis; tracheids; cell differentiation; cinnamic acid; alcohol dehydrogenase; enzyme activity; messenger RNA; gene expression; complementary DNA; Eucalyptus; Nicotiana tabacum
Abstract:
... Changes in the enzymatic activity of cinnamyl alcohol dehydrogenase (CAD) and in the expression of a gene for CAD during tracheary element (TE) differentiation were investigated in cultures of single cells isolated from the mesophyll of zinnia (Zinnia elegans). In cultures in which TE differentiation was induced (TE-inductive cultures), CAD activity increased from h 36 after the start of culture ( ...
PubMed:
9046592
PubMed Central:
PMC158157

5. Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase

Author:
Gaffe, J.; Tiznado, M.E.; Handa, A.K.
Source:
Plant physiology 1997 v.114 no.4 pp. 1547-1556
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; Nicotiana tabacum; roots; leaves; protein composition; pectinesterase; enzyme activity; hypocotyls; epicotyls; cotyledons; developmental stages; protein synthesis; genes; gene expression; amino acid sequences; messenger RNA; transgenic plants; genetic code; cell wall components; transcription (genetics)
Abstract:
... Pectin methylesterase (PME), a ubiquitous enzyme in plants, de-esterifies the methoxylated pectin in the plant cell wall. We have characterized a PME gene (designated as pmeu1) from tomato (Lycopersicon esculentum) with an expression that is higher in younger root, leaf, and fruit tissues than in older tissues. Hypocotyls and epicotyls show higher accumulation of pmeu1 transcripts compared with co ...
PubMed:
9276962
PubMed Central:
PMC158449

6. Characterization of a diffusible signal capable of inducing defense gene expression in tobacco

Author:
Chappell, J.; Levine, A.; Tenhaken, R.; Lusso, M.; Lamb, C.
Source:
Plant physiology 1997 v.113 no.2 pp. 621-629
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; Phytophthora cryptogea; plant pathogenic fungi; pathogenicity; disease resistance; genes; phytoalexins; biosynthesis; lyases; enzyme activity; measurement; gene expression; Glycine max; hydrogen peroxide; pathogenesis-related proteins; sesquiterpenoids
Abstract:
... Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynthetic enzyme in solanaceous plants) when added to fresh cell-suspension cultures. ...

7. Characterization of antisense transformed plants deficient in the tobacco anionic peroxidase

Author:
Lagrimini, L.M.; Gingas, V.; Finger, F.; Rothstein, S.; Liu, T.T.Y.
Source:
Plant physiology 1997 v.114 no.4 pp. 1187-1196
ISSN:
0032-0889
Subject:
cell growth; chemical constituents of plants; Nicotiana sylvestris; developmental stages; lignification; genes; roots; antisense RNA; phenotype; stems; cell walls; Nicotiana tabacum; genotype; peroxidase; leaves; defense mechanisms; genetic code; morphogenesis; transcription (genetics); xylem; lignin; enzyme activity; isozymes; transgenic plants; stress response; genetic variation; plant morphology
Abstract:
... On the basis of the biological compoundsthat they metabolize, plant peroxidases have long been implicated in plant growth, cell wall biogenesis, lignification, and host defenses. Transgenic tobacco (Nicotiana tabacum L.) plants that underexpress anionic peroxidase were generated using antisense RNA. The antisense RNA was found to be specific for the anionic isoenzyme and highly effective, reducing ...

8. Characterization of regions within the n-terminal 6-kilodalton domain of phytochrome A that modulate its biological activity

Author:
Jordan, E.T.; Marita, J.M.; Clough, R.C.; Vierstra, R.D.
Source:
Plant physiology 1997 v.115 no.2 pp. 693-704
ISSN:
0032-0889
Subject:
Avena sativa; seedlings; photoreceptors; red light; far-red light; phytochrome; apoproteins; plant development; alanine; serine; amino acid sequences; gene transfer; transgenic plants; Nicotiana tabacum; gene expression; genetic variation; light harvesting complex
Abstract:
... Phytochrome A (phyA) is a red/far-red (FR) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in FR light-enriched environments. We previously showed that the first 70 amino acids of the polypeptide contain at least two regions with potentially opposite functions (E.T. Jordan, J.R. Cherry, J.M. Walker, R.D. Vierstra [1996] Pl ...
PubMed:
9342873
PubMed Central:
PMC158530

9. Composition of photosystem II antenna in light-harvesting complex II antisense tobacco plants at varying irradiances

Author:
Flachman, R.
Source:
Plant physiology 1997 v.113 no.3 pp. 787-794
ISSN:
0032-0889
Subject:
Nicotiana tabacum; thylakoids; photosystem I; photosystem II; light intensity; genes; genetic code; quantitative analysis; chemical constituents of plants; light harvesting complex; transcription (genetics); phenotype
Abstract:
... Plants with genes coding for chlorophyll a/b-binding proteins of light-harvesting complex II (LHCII) in antisense orientation (Lhcb) that are characterized by severely reduced Lhcb transcript levels (below 10% of wild type) do not show a bleached phenotype due to a specific loss of the polypeptide. To produce such a phenotype, a conceptually different antisense approach was tested with a dual-func ...
PubMed:
9085572
PubMed Central:
PMC158197

10. Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues

Author:
Chen, Z.; Iyer, S.; Caplan, A.; Klessig, D.F.; Fan, B.
Source:
Plant physiology 1997 v.114 no.1 pp. 193-201
ISSN:
0032-0889
Subject:
Oryza sativa; Nicotiana tabacum; shoots; roots; cell suspension culture; leaves; salicylic acid; biosynthesis; quantitative analysis; catalase; enzyme activity; genes; gene expression; genetic code; chemical constituents of plants
Abstract:
... We previously proposed that salicylic acid (SA)-sensitive catalases serve as biological targets of SA in plant defense responses. To further examine the role of SA-sensitive catalases, we have analyzed the relationship between SA levels and SA sensitivity of catalases in different rice (Oryza sativa) tissues. We show here that, whereas rice shoots contain extremely high levels of free SA, as previ ...

11. Differential gene expression in response to auxin treatment in the wild type and rac, an adventitious rooting-incompetent mutant of tobacco

Author:
Lund, S.T.; Smith, A.G.; Hackett, W.P.
Source:
Plant physiology 1997 v.114 no.4 pp. 1197-1206
ISSN:
0032-0889
Subject:
cortex; messenger RNA; cell growth; transgenic plants; developmental stages; cell division; adventitious roots; gene expression; phloem; rooting; genes; Nicotiana tabacum; mutants; auxins; genotype; plant cuttings; histochemistry; callus
Abstract:
... Histological analyses of auxin-treated cuttings from the wildtype and the rac mutant of tobacco (Nicotiana tabacum cv Xanthii) previously revealed that some rac phloem parenchyma or inner cortical parenchyma cells form callus in response to exogenous auxin treatment but these cells never undergo the organized divisions associated with adventitious root initiation in the wild type. Here we report t ...

12. Diverse amino acid residues function within the type 1 peroxisomal targeting signal. Implications for the role of accessory residues upstream of the type 1 peroxisomal targeting signal

Author:
Mullen, R.T.; Lee, M.S.; Flynn, C.R.; Trelease, R.N.
Source:
Plant physiology 1997 v.115 no.3 pp. 881-889
ISSN:
0032-0889
Subject:
lysine; leucine; peroxisomes; serine; glyoxylate cycle; cytosol; binding proteins; translocation (plant physiology); Nicotiana tabacum; nucleotide sequences; chloramphenicol acetyltransferase; glyoxysomes; cell suspension culture
Abstract:
... The purpose of this study was to determine whether the plant type 1 peroxisomal targeting signal (PTS1) utilizes amino acid residues that do not strictly adhere to the serine-lysine-leucine (SKL) motif (small-basic-hydrophobic residues). Selected residues were appended to the C terminus of chloramphenicol acetyltransferase (CAT) and were tested for their ability to target CAT fusion proteins to gl ...
PubMed:
9390426
PubMed Central:
PMC158551

13. Elevated CO2 effects during leaf ontogeny: a new perspective on acclimation

Author:
Miller, A.; Tsai, C.H.; Hemphill, D.; Endres, M.; Rodermel, S.; Spalding, M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1195-1200
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; carbon dioxide; developmental stages; net assimilation rate; acclimation; ontogeny; Nicotiana tabacum; photosynthesis; enzyme activity; leaves
Abstract:
... For many plants growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role that leaf ontogeny plays in the acclimation response, we monitored photosynthesis and some related parameters at short intervals throughout the ontogenetic development of tobacco (Nicotiana tabacum L.) leaves under ambient (350 microliters L-1)- and high (950 microliters L-1)-CO2 conditions. The pa ...

14. Expression of an aspartate kinase homoserine dehydrogenase gene is subject to specific spatial and temporal regulation in vegetative tissues, flowers, and developing seeds

Author:
Zhu-Shimoni, J.X.; Lev-Yadun, S.; Matthews, B.; Galili, G.
Source:
Plant physiology 1997 v.113 no.3 pp. 695-706
ISSN:
0032-0889
Subject:
chemical reactions; developmental stages; genes; phosphotransferases (kinases); pollen; gene expression; Nicotiana tabacum; leaves; Arabidopsis thaliana; aspartic acid; enzyme activity; histochemistry; gene transfer; homoserine; biosynthesis; transgenic plants; flowers; biochemical pathways; oxidoreductases; plant morphology; seed development
Abstract:
... Although theregulation of amino acid synthesis has been studied extensively at the biochemical level, it is still not known how genes encoding amino acid biosynthesis enzymes are regulated during plant development. In the present report, we have used the beta-glucuronidase (GUS) reporter gene to study the regulation of expression of an Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ...

15. Expression of pokeweed antiviral protein in transgenic plants induced virus resistance in grafted wild-type plants independently of salicylic acid accumulation and pathogenesis-related protein synthesis

Author:
Smironv, S.; Shulaev, V.; Tumer, N.E.
Source:
Plant physiology 1997 v.114 no.3 pp. 1113-1121
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Tobacco mosaic virus; Potato virus X; pathogenicity; disease resistance; plant breeding; gene transfer; transgenic plants; gene expression; genes; protein synthesis; Phytolacca americana; salicylic acid; quantitative analysis; pathogenesis-related proteins; chemical constituents of plants
Abstract:
... Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. Transgenic tobacco (Nicotiana tabacum) plants expressing PAP or a variant (PAP-v) were shown to be resistant to a broad spectrum of plant viruses. Expression of PAP-v in transg ...

16. A G-box-binding protein from soybean binds to the E1 auxin-response element in the soybean GH3 promoter and contains a proline-rich repression domain

Author:
Liu, Z.B.; Hagen, G.; Guilfoyle, T.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 397-407
ISSN:
0032-0889
Subject:
protoplasts; gene transfer; Glycine max; leucine; genetic code; transgenic plants; Daucus carota; proline; luciferase; binding proteins; gene expression; plasmids; auxins; Nicotiana tabacum; transcription (genetics); reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plan ...

17. Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane

Author:
Yan, X.H.; Gonzales, R.A.; Wagner, G.J.
Source:
Plant physiology 1997 v.115 no.3 pp. 915-924
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Manduca sexta; Nicotiana tabacum; leaves; callus; endoplasmic reticulum; sucrose; biochemical pathways; protein composition; beta-glucuronidase; enzyme activity; reporter genes; transgenic plants; gene expression; cytosol; gene transfer; genetic vectors; genetic transformation; plasma membrane; translocation (plant physiology); plant secretions and exudates
Abstract:
... Signal sequences and endoplasmic reticulum (ER)retention signals are known to play central roles in targeting and translocation in the secretory pathway, but molecular aspects about their involvement are poorly understood. We tested the effectiveness of deduced signal sequences from various genes (hydroxyproline-rich glycoprotein [HRGP] from Phaseolus vulgaris; Serpin from Manduca sexta) to direct ...
PubMed:
9390428
PubMed Central:
PMC158555

18. Heat treatment results in a loss of transgene-encoded activities in several tobacco lines

Author:
Neumann, K.; Droge-Laser, W.; Kohne, S.; Broer, I.
Source:
Plant physiology 1997 v.115 no.3 pp. 939-947
ISSN:
0032-0889
Subject:
seedlings; luciferase; neomycin; heritability; heat treatment; phenotype; luminescence; Agrobacterium radiobacter; nopaline; heat stress; gene expression; Nicotiana tabacum; genetic code; ligases; phosphotransferases (phosphomutases); temperature; enzyme activity; line differences; genetic transformation; gene transfer; protein synthesis; transgenic plants; stress response; reporter genes; RNA; plant morphology
Abstract:
... Heat treatment (37 degrees C) of transgenic tobacco (Nicotiana tabacum) plants led to a reversible reduction or complete loss of transgene-encoded activities in about 40% of 10 independent transformants carrying the luciferase-coding region fused to the 355 cauliflower mosaic virus or the soybean small subunit promoter and the nopaline synthase promoter driving the neomycin phosphotransferase gene ...
PubMed:
9390430
PubMed Central:
PMC158557

19. Hypoosmotic shock induces increases in cytosolic Ca2+ in tobacco suspension-culture cells

Author:
Takahashi, K.; Isobe, M.; Knight, M.R.; Trewavas, A.J.; Muto, S.
Source:
Plant physiology 1997 v.113 no.2 pp. 587-594
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; osmotic pressure; cytosol; calcium; luminescence; measurement; transgenic plants; gene expression; stress response
Abstract:
... Hypoosmotic shock treatment increased cytosolic Ca2+ ion concentration ([Ca2+]cyt) in tobacco (Nicotiana tabacum) suspension-culture cells. [Ca2+]cyt measurements were made by genetically transforming these cells to express apoaequorin and by reconstituting the Ca2+-dependent photoprotein, aequorin, in the cytosol by incubation with chemically synthesized coelenterazine. Measurement of Ca2+-depend ...

20. Identification of a soluble, high-affinity salicylic acid-binding protein in tobacco

Author:
Du, H.; Klessig, D.F.
Source:
Plant physiology 1997 v.113 no.4 pp. 1319-1327
ISSN:
0032-0889
Subject:
catalase; azoles; binding proteins; physicochemical properties; Nicotiana tabacum; salicylic acid; leaves; disease resistance; receptors; protein composition; defense mechanisms
Abstract:
... Salicylic acid (SA) is a key component in the signal transduction pathway(s), leading to the activation of certain defense responses in plants after pathogen attack. Previous studies have identified several proteins, including catalase and ascorbate peroxidase, through which the SA signal might act. Here we describe a new SA-binding protein. This soluble protein is present in low abundance in toba ...

21. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

22. Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals

Author:
Smith, H.M.S.; Hicks, G.R.; Raikhel, N.V.
Source:
Plant physiology 1997 v.114 no.2 pp. 411-417
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Nicotiana tabacum; protein composition; protein transport; receptors; binding proteins; protoplasts; plant anatomy; plasma membrane
Abstract:
... Protein import into the nucleus is a two-step process. In vitro import systems from vertebrate cell extracts have shown that several soluble factors are required. One of these factors is the receptor importin alpha, which binds to nuclear localization signals (NLS) in vitro. We previously cloned an importin alpha homolog from Arabidopsis thaliana (At-IMP alpha) and demonstrated that this protein w ...
PubMed:
9193081
PubMed Central:
PMC158320

23. Increased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts

Author:
Shen, B.; Jensen, R.G.; Bohnert, H.J.
Source:
Plant physiology 1997 v.113 no.4 pp. 1177-1183
ISSN:
0032-0889
Subject:
Nicotiana tabacum; oxygen; leaves; chloroplasts; mannitol; biosynthesis; chlorophyll; quantitative analysis; photosynthesis; net assimilation rate; gene transfer; bacteria; genes; oxidoreductases; enzyme activity; wild relatives; transgenic plants; gene expression; chemical constituents of plants; genotype; reactive oxygen species; stress response
Abstract:
... To investigate the potential role of a polyol, mannitol, in oxidative stress protection, a bacterial mannitol-1-phosphate dehydrogenase gene was targeted to chloroplasts by the addition of an amino-terminal transit peptide. Transgenic tobacco (Nicotiana tabacum) lines accumulate mannitol at concentrations ranging from 2.5 to 7 micromoles/g fresh weight. Line BS1-31 accumulated approximately 100 mM ...
PubMed:
9112772
PubMed Central:
PMC158240

24. Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L

Author:
Sheveleva, E.; Chmara, W.; Bohnert, H.J.; Jensen, R.G.
Source:
Plant physiology 1997 v.115 no.3 pp. 1211-1219
ISSN:
0032-0889
Subject:
chemical constituents of plants; drought tolerance; drought; measurement; metabolites; glucose; proline; Nicotiana tabacum; fructose; photosynthesis; quantitative analysis; complementary DNA; sucrose; myo-inositol; net assimilation rate; enzyme activity; salt tolerance; salinity; transgenic plants; stress response; diurnal variation; genetic variation; stomata; transferases; leaf conductance
Abstract:
... A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1. During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 micromoles g-1 fresh weight In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type. ...

25. Influence of environmental stress on biomass partitioning in transgenic tobacco plants expressing the movement protein of tobacco mosaic virus

Author:
Balachandran, S.; Hull, R.J.; Martins, R.A.; Vaadia, Y.; Lucas, W.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 475-481
ISSN:
0032-0889
Subject:
Nicotiana tabacum; transgenic plants; gene expression; genetic vectors; Tobacco mosaic virus; viral proteins; nutrient transport; roots; shoots; water stress; nutrient deficiencies; nitrogen; potassium; phosphorus; source-sink relationships; genetic variation; translocation (plant physiology); genotype; photosynthates; stress response
Abstract:
... The influence of various environmental factors on biomass partitioning between shoots and roots in transgenic tobacco (Nicotiana tabacum) plants expressing the movement protein (MP) of tobacco mosaic virus (TMV) was investigated. TMV-MP-expressing transgenic plants exhibited a root-to-shoot ratio that was approximately 40% below that of transgenic vector control plants. When transgenic plants expr ...

26. Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants

Author:
Goddijn, O.J.M.; Verwoerd, T.C.; Voogd, E.; Krutwagen, R.W.H.H.; Graaf, P.T.H.M. de.; Poels, J.; Dun, K. van.; Ponstein, A.S.; Dammn, B.; Pen, J.
Source:
Plant physiology 1997 v.113 no.1 pp. 181-190
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Solanum tuberosum; tubers; leaves; trehalose; biosynthesis; quantitative analysis; hexosyltransferases; phosphoric monoester hydrolases; trehalase; enzyme activity; enzyme inhibitors; plant development; genetic engineering; transgenic plants; genes; gene transfer; gene expression; cultivars; genetic variation
Abstract:
... As a first step toward the exploitation of the disaccharide trehalose as a stress-protective and preservative agent in plants, we engineered trehalose biosynthesis in tobacco (Nicotiana tabacum) and potato (Solanum tuberosum) by introducing the otsA and otsB genes from Escherichia coli, which encode trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase, respectively. In leaves of tr ...
PubMed:
9008394
PubMed Central:
PMC158129

27. Involvement of thylakoid overenergization in tentoxin-induced chlorosis in Nicotiana spp

Author:
Holland, N.; Evron, Y.; Jansen, M.A.K.; Edelman, M.; Pick, U.
Source:
Plant physiology 1997 v.114 no.3 pp. 887-892
ISSN:
0032-0889
Subject:
seedlings; plant pathogenic fungi; Nicotiana plumbaginifolia; dose response; Nicotiana tabacum; Alternaria alternata; chlorosis; oxygen; adenosinetriphosphatase; superoxide dismutase; adenosine triphosphate; pathogenicity; enzyme activity; gramicidin; ascorbic acid; biosynthesis; free radicals; thylakoids; transgenic plants; photophosphorylation; mutants
Abstract:
... The purpose of this work was to clarify the mechanism of tentoxin-induced chlorosis inNicotiana spp. seedlings. We found that chlorosis does not correlate with the inhibition of chloroplast ATP synthesis in vivo, since it occurs at tentoxin concentrations far higher than that required for the inhibition of photophosphorylation measured in the same seedlings. However, tentoxin-induced chlorosis doe ...

28. Local and systemic responses to antioxidants to tobacco mosaic virus infection and to salicylic acid to tobacco. Role in systemic acquired resistance

Author:
Fodor, J.; Gullner, G.; Adam, A.L.; Barna, B.; Komives, T.; Kiraly, Z.
Source:
Plant physiology 1997 v.114 no.4 pp. 1443-1451
ISSN:
0032-0889
Subject:
dehydroascorbic acid; necrosis; salicylic acid; Tobacco mosaic virus; glutathione; dose response; glutathione transferase; Nicotiana tabacum; disease resistance; leaves; quantitative analysis; superoxide dismutase; pathogenicity; enzyme activity; isozymes; antioxidants; ascorbic acid; glutathione-disulfide reductase; catalase; chemical constituents of plants
Abstract:
... Changes in ascorbate and glutathione levels and in activitiesof ascorbate peroxidase, catalase, dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and superoxide dismutase (SOD) were investigated in tobacco mosaic virus (TMV)-inoculated lower leaves and in noninoculated upper leaves of Nicotiana tabacum L. cv Xanthi-nc. In separate experiments the effec ...

29. Mannitol protects against oxidation by hydroxyl radicals

Author:
Shen, B.; Jensen, R.G.; Bohnert, H.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 527-532
ISSN:
0032-0889
Subject:
drought; mannitol; phosphotransferases (kinases); betaine; metabolites; glutathione; Nicotiana tabacum; oxidation; Calvin cycle; superoxide dismutase; enzyme inhibitors; enzyme activity; ribulose-bisphosphate carboxylase; free radicals; thylakoids; catalase; cooling; stress response; phenylalanine
Abstract:
... Hydroxyl radicals may be responsible for oxidative damage during drought or chilling stress. We have shown that the presence of mannitol in chloroplasts can protect plants against oxidative damage by hydroxyl radicals (B. Shen, R.G. Jensen, H.J. Bohnert [1997] Plant Physiol 113:1177-1183). Here we identify one of the target enzymes that may be protected by mannitol. Isolated thylakoids in the pres ...

30. Molecular cloning and further characterization of a portable plant vacuolar sorting receptor

Author:
Paris, N.; Rogers, S.W.; Jiang, L.W.; Kirsch, T.; Beevers, L.; Phillips, T.E.; Rogers, J.C.
Source:
Plant physiology 1997 v.115 no.1 pp. 29-39
ISSN:
0032-0889
Subject:
Oryza sativa; vacuoles; cell membranes; genes; Pisum sativum; proline; binding proteins; gene expression; Nicotiana tabacum; protein composition; Arabidopsis thaliana; Zea mays; complementary DNA; arginine; amino acid sequences; isoleucine; immunocytochemistry; Golgi apparatus; asparagine; cell suspension culture
Abstract:
... BP-80 is a type I integral membrane protein abundant in pea (Pisum sativum) clathrin-coated vesicles (CCVs) that binds with high affinity to vacuole-targeting determinants containing asparagine-proline-isoleucine-arginine. Here we present results from cDNA cloning and studies of its intracellular localization. Its sequence and sequences of homologs from Arabidopsis, rice (Oryza sativa), and maize ...

31. Molecular genetic evidence of the ability of alternative oxidase to support respiratory carbon metabolism

Author:
Vanlerberghe, G.C.; Vanlerberghe, A.E.; McIntosh, L.
Source:
Plant physiology 1997 v.113 no.2 pp. 657-661
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; cell growth; mitochondria; carbohydrate metabolism; carbon; oxidoreductases; enzyme activity; genes; transgenic plants; cell lines; genetic variation; electron transfer
Abstract:
... With the cytochrome pathway inhibited, AOX was able to support considerable growth of cultured tobacco (Nicotiana tabacum cv Petit Havana SR1) cells but the efficiency of carbon utilization decreased dramatically. Antisense cells with decreased AOX protein did not grow, whereas sense cells with elevated AOX protein had higher growth and respiration rates than the wild type. In antisense cells a la ...

32. The N-terminal propeptide of the precursor of sporamin acts as a vacuole-targeting signal even at the C terminus of the mature part in tobacco cells

Author:
Koide, Y.; Hirano, H.; Matsuoka, K.; Nakamura, K.
Source:
Plant physiology 1997 v.114 no.3 pp. 863-870
ISSN:
0032-0889
Subject:
Nicotiana tabacum; vacuoles; peptides; protein synthesis; protein transport; asparagine; proline; isoleucine; arginine; leucine; amino acid sequences; genetic variation; mutants
Abstract:
... An asparagine-proline-isoleucine-arginine-leucine (NPIRL) and its related sequences in the N-terminal propeptides (NTPP) of several plant vacuolar proteins, including that of sporamin from sweet potato (SPO) function as vacuole-targeting determinants in a manner that is distinct from the vacuole-targeting determinant in the CTPPs of other plant vacuolar proteins. When the mutant precursor to spora ...

33. Overproduction of ascorbate peroxidase in the tobacco chloroplast does not provide protection against ozone

Author:
Torsethaugen, G.; Pitcher, L.H.; Zilinskas, B.A.; Pell, E.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 529-537
ISSN:
0032-0889
Subject:
genetic transformation; gene transfer; biosynthesis; phytotoxicity; gas exchange; transgenic plants; Agrobacterium radiobacter; stress response; ozone; measurement; Nicotiana tabacum; enzyme activity; peroxidase; antioxidants; chloroplasts; ascorbic acid
Abstract:
... Transgenic tobacco (Nicotiana tabacum cv Bel W3) plants were used to test the hypothesis that protection from O3 injury could be conferred by overproduction of ascorbate peroxidase (APX) in the chloroplast. The 10-fold increase in soluble APX activity in the chloroplast was expected to alleviate an implied increase in oxidative potential and prevent damage caused by O3. Three different O3 exposure ...

34. Partial characterization of glutathione S-transferases from wheat (Triticum spp.) and purification of a safener-induced glutathione S-transferase from Triticum tauschii

Author:
Riechers, D.E.; Irzyk, G.P.; Jones, S.S.; Fuerst, E.P.
Source:
Plant physiology 1997 v.114 no.4 pp. 1461-1470
ISSN:
0032-0889
Subject:
Zea mays; protein composition; diploidy; glutathione transferase; herbicide safeners; physicochemical properties; amino acid sequences; measurement; Nicotiana tabacum; Triticum aestivum; Triticum turgidum subsp. durum; leaves; dimethenamid; enzyme activity; isozymes; hexaploidy; shoots
Abstract:
... Hexaploid wheat (Triticum aestivum L.) has very low constitutive glutathione S-transferase (GST) activity when assayed with the chloroacetamide herbicide dimethenamid as a substrate, which may account for its low tolerance to dimethenamid in the field. Treatment of seeds with the herbicide safener fluxofenim increased the total GST activity extracted from T. aestivum shoots 9-fold when assayed wit ...
PubMed:
9276955
PubMed Central:
PMC158439

35. Promoter analysis and expression of a phospholipase D gene from castor bean

Author:
Xu, L.; Zheng, S.; Zheng, L.; Wang, X.
Source:
Plant physiology 1997 v.115 no.2 pp. 387-395
ISSN:
0032-0889
Subject:
cell growth; corolla; seedlings; genes; cell differentiation; pollen; promoter regions; calyx; phosphoric diester hydrolases; gynoecium; hypocotyls; meristems; Ricinus communis; gene expression; Nicotiana tabacum; leaves; messenger RNA; buds; histology; enzyme activity; vascular bundles; gene transfer; transgenic plants; sexual reproduction; beta-glucuronidase
Abstract:
... The expression of a castor bean (Ricinus communis L.) phospholipase D (PLD; EC 3.1.4.4) gene has been studied by examining its promoter activity in transgenic tobacco (Nicotiana tabacum) carrying a PLD promoter-glucuronidase transgene and by monitoring the levels of PLD mRNA in castor bean. Sequence and the 5' truncation analyses revealed that the 5' flanking region from nucleotide -1200 to -730 i ...
PubMed:
9342861
PubMed Central:
PMC158496

36. Quantum yields and rate constants of photochemical and nonphotochemical excitation quenching. Experiment and model

Author:
Laisk, A.; Oja, V.; Rasulov, B.; Eichelmann, H.; Sumberg, A.
Source:
Plant physiology 1997 v.115 no.2 pp. 803-815
ISSN:
0032-0889
Subject:
Helianthus annuus; ion transport; light intensity; photoinhibition; measurement; carbon dioxide; dose response; Gossypium hirsutum; light harvesting complex; electron transfer; Sorghum bicolor; Nicotiana tabacum; leaves; Amaranthus cruentus; mathematical models; equations; net assimilation rate; temperature; prediction; cytochrome b
Abstract:
... Sunflower (Helianthus annuus L.), cotton (Gossypium hirsutum L.), tobacco (Nicotiana tabacum L.), sorghum (Sorghum bicolor Moench.), amaranth (Amaranthus cruentus L.), and cytochrome b6f complex-deficient transgenic tobacco leaves were used to test the response of plants exposed to different light intensities and CO2 concentrations before and after photoinhibition at 4000 micromoles photons m-2 s- ...

37. RNase activity prevents the growth of a fungal pathogen in tobacco leaves and increases upon induction of systemic acquired resistance with elicitin

Author:
Galiana, E.; Bonnet, P.; Conrod, S.; Keller, H.; Panabieres, F.; Ponchet, M.; Poupet, A.; Ricci, P.
Source:
Plant physiology 1997 v.115 no.4 pp. 1557-1567
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Phytophthora cryptogea; Phytophthora nicotianae var. parasitica; plant pathogenic fungi; disease resistance; induced resistance; pathogenicity; necrosis; leaves; hyphae; cell growth; ribonucleases; enzyme activity; genes; genetic code; gene expression; transcription (genetics)
Abstract:
... The hypersensitive response and systemic acquired resistance (SAR) can be induced in tobacco (Nicotiana tabacum L.) plants by cryptogein, an elicitin secreted by Phytophthora cryptogea. Stem application of cryptogein leads to the establishment of acquired resistance to subsequent leaf infection with Phytophthora parasitica var nicotianae, the agent of the tobacco black shank disease. We have studi ...
PubMed:
9414563
PubMed Central:
PMC158622

38. Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora

Author:
Adam, A.L.; Pike, S.; Hoyos, M.E.; Stone, J.M.; Walker, J.C.; Novacky, A.
Source:
Plant physiology 1997 v.115 no.2 pp. 853-861
ISSN:
0032-0889
Subject:
ethylene glycol tetraacetic acid; Erwinia amylovora; biosynthesis; cycloheximide; chelating agents; phosphoric monoester hydrolases; lanthanum; tyrosine; enzyme inhibitors; bacterial proteins; Nicotiana tabacum; myelin sheath; pathogenesis; phosphorylation; enzyme activity; leaves; measurement
Abstract:
... Harpins are bacterial protein elicitors that induce hypersensitive response-like necrosis when infiltrated into nonhost plants such as tobacco (Nicotiana tabacum L.) (Z.-M. Wei, R.J. Laby, C.H. Zumoff, D.W. Bauer, S.Y. He, A. Collmer, S.V. Beer [1992] Science 257: 85-88). Activity of a 49-kD Mg2+-dependent and Ca2+-independent kinase in tobacco leaves increased 50-fold 15 min after infiltration of ...

39. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia-lyase or cinnamate 4-hydroxylase

Author:
Sewalt, V.J.H.; Ni, W.T.; Blount, J.W.; Jung, H.G.; Masoud, S.A.; Howles, P.A.; Lamb, C.; Dixon, R.A.
Source:
Plant physiology 1997 v.115 no.1 pp. 41-50
ISSN:
0032-0889
Subject:
biosynthesis; phenylalanine ammonia-lyase; stems; chemical reactions; transgenic plants; biochemical pathways; cinnamic acid; gene expression; histochemistry; Nicotiana tabacum; genes; phenylalanine; chemical constituents of plants; enzyme activity; lignin; oxygenases
Abstract:
... We analyzed lignin content and composition in transgenic tobacco (Nicotiana tabacum) lines altered in the expression of the early phenylpropanoid biosynthetic enzymes L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase (C4H). The reduction of C4H activity by antisense expression or sense suppression resulted in reduced levels of Klason lignin, accompanied by a decreased syringyl/guaiacyl mon ...

40. Reduction of uroporphyrinogen decarboxylase by antisense RNA expression affects activities of other enzymes involved in tetrapyrrole biosynthesis and leads to light-dependent necrosis

Author:
Mock, H.P.; Grimm, B.
Source:
Plant physiology 1997 v.113 no.4 pp. 1101-1112
ISSN:
0032-0889
Subject:
gene transfer; biosynthesis; antisense RNA; complementary DNA; genetic code; wild relatives; porphyrins; transgenic plants; necrosis; phenotype; gene expression; Nicotiana tabacum; growth retardation; carboxy-lyases; leaves; genetic variation; enzyme activity; plant morphology; light
Abstract:
... We introduced a full-length cDNA sequence encoding tobacco (Nicotiana tabacum) uroporphyrinogen III decarboxylase (UROD; EC 4.1.1.37) in reverse orientation under the control of a cauliflower mosaic virus 35S promoter derivative into the tobacco genome to study the effects of deregulated UROD expression on tetrapyrrole biosynthesis. Transformants with reduced UROD activity were characterized by st ...

41. Regulation of sesquiterpene cyclase gene expression. Characterization of an elicitor- and pathogen-inducible promoter

Author:
Yin, S.; Mei, L.; Newman, J.; Back, K.; Chappell, J.
Source:
Plant physiology 1997 v.115 no.2 pp. 437-451
ISSN:
0032-0889
Subject:
sesquiterpenoids; plant pathogenic fungi; salicylic acid; promoter regions; jasmonic acid; hydrogen peroxide; gene expression; Nicotiana tabacum; histology; transcription (genetics); pathogenicity; Phytophthora nicotianae var. parasitica; enzyme activity; genetic transformation; gene transfer; biosynthesis; plant anatomy; transgenic plants; Pseudomonas syringae pv. syringae; reporter genes; beta-glucuronidase
Abstract:
... The promoter for a tobacco (Nicotiana tabacum) sesquiterpene cyclase gene, a key regulatory step in sesquiterpene phytoalexin biosynthesis, has been analyzed. The EAS4 promoter was fused to the beta-glucuronidase (GUS) reporter gene, and the temporal and spatial expression patterns of GUS activity were examined in stably transformed plants and in transient expression assays using electroporated pr ...
PubMed:
9342864
PubMed Central:
PMC158501

42. Restriction of chlorophyll synthesis due to expression of glutamate 1-semialdehyde aminotransferase antisense RNA does not reduce the light-harvesting antenna size in tobacco

Author:
Hartel, H.; Kruse, E.; Grimm, B.
Source:
Plant physiology 1997 v.113 no.4 pp. 1113-1124
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; carotenoids; photosystem II; quantitative analysis; chlorophyll; binding proteins; biosynthesis; glutamates; aldehydes; transaminases; enzyme activity; gene expression; antisense RNA; transgenic plants; wild relatives; plant morphology; leaf area; chemical constituents of plants; light harvesting complex; phenotype
Abstract:
... The formation of 5-aminolevulinate is a key regulatory step in tetrapyrrole biosynthesis. In higher plants, glutamate 1-semialdehyde aminotransferase (GSA-AT) catalyzes the last step in the sequential conversion of glutamate to 5-aminolevulinate. Antisense RNA synthesis for GSA-AT leads to reduced GSA-AT protein levels in tobacco (Nicotiana tabacum L.) plants. We have used these transgenic plants ...

43. Ribulose-1,5-bisphosphate carboxylase/oxygenase activase deficiency delays senescence of ribulose-1,5-bisphosphate carboxylase/oxygenase but progressively impairs its catalysis during tobacco leaf development

Author:
He, Z.; Von Caemmerer, S.; Hudson, G.S.; Price, G.D.; Badger, M.R.; Andrews, T.J.
Source:
Plant physiology 1997 v.115 no.4 pp. 1569-1570
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; carbon dioxide; plant development; messenger RNA; protein content; chemical constituents of plants; antisense DNA; transgenic plants; developmental stages; net assimilation rate; gene expression; Nicotiana tabacum; glyceraldehyde-3-phosphate dehydrogenase; genes; genetic variation; photosynthesis; enzyme activity; chlorophyll; leaves; measurement
Abstract:
... Transgenic tobacco (Nicotiana tabacum L. cv W38) plants with an antisense gene directed against the mRNA of ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) activase grew more slowly than wild-type plants in a CO2-enriched atmosphere, but eventually attained the same height and number of leaves. Compared with the wild type, the anti-activase plants had reduced CO2 assimilation rates, norma ...
PubMed:
9414564
PubMed Central:
PMC158623

44. Sequencing, genomic organization, and preliminary promoter analysis of a black cherry (R)-(+)-mandelonitrile lyase gene

Author:
Hu, Z.; Poulton, J.E.
Source:
Plant physiology 1997 v.115 no.4 pp. 1359-1369
ISSN:
0032-0889
Subject:
developmental stages; cyanogenesis; promoter regions; Agrobacterium; gene expression; Nicotiana tabacum; complementary DNA; amino acid sequences; enzyme activity; histochemistry; gene transfer; transgenic plants; aldehyde-lyases; embryo (plant); reporter genes; genomics; Prunus serotina; beta-glucuronidase; seed development
Abstract:
... The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its corresponding cDNA (MDL3) were isolated from black cherry (Prunus serotina) and characterized. The mdl3 gene contains 2292 bp of the 5' flanking region, the entire coding region, and 300 bp of the 3' flanking region. The coding region is interrupte ...
PubMed:
9414550
PubMed Central:
PMC158601

45. Source strength regulates an early phase transition of tobacco shoot morphogenesis

Author:
Tsai, C.H.; Miller, A.; Spalding, M.; Rodermel, S.
Source:
Plant physiology 1997 v.115 no.3 pp. 907-914
ISSN:
0032-0889
Subject:
Nicotiana tabacum; shoots; morphogenesis; source-sink relationships; developmental stages; internodes; leaf area; photosynthesis; net assimilation rate; ribulose-bisphosphate carboxylase; enzyme activity; mutants; genetic variation; root shoot ratio
Abstract:
... We have taken advantage of specific reductions in the ribulose-1,5-bisphosphate carboxylase/oxygenase concentration in rbcS antisense mutants of tobacco (Nicotiana tabacum L.) to assess the contribution of source strength (carbohydrate production) to the control of shoot development. Wild-type and antisense plants undergo distinct phases of shoot development that can be distinguished from one anot ...

46. Structural characterization of modified lignin in transgenic tobacco plants in which the activity of 4-coumarate:coenzyme A ligase is depressed

Author:
Kajita, S.; Hishiyama, S.; Tomimura, Y.; Katayama, Y.; Omori, S.
Source:
Plant physiology 1997 v.114 no.3 pp. 871-879
ISSN:
0032-0889
Subject:
Nicotiana tabacum; xylem; lignin; quantitative analysis; biosynthesis; ferulic acid; sinapic acid; p-coumaric acid; coenzyme A; ligases; enzyme activity; transgenic plants; gene expression
Abstract:
... Transgenic tobacco (Nicotiana tabacum L.) plants in which the activity of 4-coumarate:coenzyme A ligase is very low contain a novel lignin in their xylem. Details of changes inhydroxycinnamic acids bound to cell walls and in the structure of the novel lignin were identified by base hydrolysis, alkaline nitrobenzene oxidation, pyrolysis-gas chromatography, and 13C-nuclear magnetic resonance analysi ...

47. Transgenic tobacco plants expressing pea chloroplast Nmdh c DNA in sense and antisense orientation. Effects on NADP-malate dehydrogenase level, stability of transformants, and plant growth

Author:
Faske, M.; Backhausen, J.E.; Sendker, M.; Singer-Bayrle, M.; Scheibe, R.; Schaewen, A. von.
Source:
Plant physiology 1997 v.115 no.2 pp. 705-715
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Pisum sativum; leaves; chloroplasts; genes; gene transfer; genetic code; complementary DNA; antisense DNA; malic acid; alcohol oxidoreductases; transgenic plants; gene expression; measurement; enzyme activity; genetic variation; plant development; genotype; NAD (coenzyme)
Abstract:
... A full-length cDNA encoding light-activated chloroplast NADP-malate dehydrogenase (NADP-MDH) (EC 1.1.1.82) from pea (Pisum sativum L.) was introduced in the sense and antisense orientation into tobacco (Nicotiana tabacum L.). Transgenic plants with decreased or increased expression levels were obtained. Because of substantial age-dependent differences in individual leaves of a single plant, standa ...

48. Transgenically expressed betaine aldehyde dehydrogenase efficiently catalyzes oxidation of dimethylsulfoniopropionaldehyde and omega-aminoaldehydes

Author:
Trossat, C.; Rathinasabapathi, B.; Hanson, A.D.
Source:
Plant physiology 1997 v.113 no.4 pp. 1457-1461
ISSN:
0032-0889
Subject:
genetic transformation; Beta vulgaris; gene transfer; biosynthesis; complementary DNA; transgenic plants; aldehyde dehydrogenase; chemical structure; gene expression; Nicotiana tabacum; oxidation; catalytic activity; betaine; aldehydes; leaves
Abstract:
... Tobacco (Nicotianum tabacum L.) plants engineered to express a sugar beet (Beta vulgaris L.) betaine aldehyde dehydrogenase (BADH) cDNA acquired not only BADH activity, but also three other aldehyde dehydrogenase activities (those measured with 3-dimethylsulfoniopropionaldehyde, 3-aminopropionaldehyde, and 4-aminobutyraldehyde, all of which are natural products). This shows that BADH is not, as be ...

49. beta-1,3-glucanase is cryoprotective in vitro and is accumulated in leaves during cold acclimation

Author:
Hincha, D.K.; Meins, F. Jr.; Schmitt, J.M.
Source:
Plant physiology 1997 v.114 no.3 pp. 1077-1083
ISSN:
0032-0889
Subject:
Spinacia oleracea; Nicotiana tabacum; Brassica oleracea; leaves; in vitro culture; thylakoids; frost injury; cryoprotectants; beta-glucanase; isozymes; protein synthesis; acclimation; cold tolerance; osmotic pressure; solutes; stress response
Abstract:
... We have used isolated spinach (Spinacea oleracea L.) thylakoid membranes to investigate the possible cryoprotective properties of class I beta-1,3-glucanase (1,3-beta-D-glucan 3-glucanohydrolase; EC 3.2.1.39) and chitinase. Class I beta-1,3-glucanase that was purified from tobacco (Nicotiana tabacum L.) protected thylakoids against freeze-thaw injury in our in vitro assays, whereas class I chitina ...

50. cDNA clones encoding 1,3-beta-glucanase and a fimbrin-like cytoskeletal protein are induced by Al toxicity in wheat roots

Author:
Cruz-Ortega, R.; Cushman, J.C.; Ownby, J.D.
Source:
Plant physiology 1997 v.114 no.4 pp. 1453-1460
ISSN:
0032-0889
Subject:
Triticum aestivum; Brassica napus; Arabidopsis thaliana; Hordeum vulgare; Nicotiana tabacum; Pisum sativum; roots; aluminum; phytotoxicity; protein synthesis; actin; molecular weight; isoelectric point; beta-glucanase; cytoskeleton; protein composition; genetic code; complementary DNA; genes; gene expression; clones; cultivars; genetic variation; amino acid sequences; messenger RNA; stress response
Abstract:
... A cDNA library made from mRNA of Al-treated roots of an Al-sensitive wheat (Triticum aestivum cv Victory) cultivar was screened with a degenerate oligonucleotide probe derived from the partial amino acid sequence of the Al-induced protein TAl-18. Of seven clones that initially hybridized with the probe, one encoded a novel 1,3-beta-glucanase having a calculated molecular weight of 46.3 and an isoe ...
PubMed:
9276954
PubMed Central:
PMC158438

51. The effects of salicylic acid and tobacco mosaic virus infection on the alternative oxidase of tobacco

Author:
Lennon, A.M.; Neuenschwander, U.H.; Ribas-Carbo, M.; Giles, L.; Ryals, J.A.; Siedow, J.N.
Source:
Plant physiology 1997 v.115 no.2 pp. 783-791
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell suspension culture; mitochondria; Tobacco mosaic virus; pathogenicity; disease resistance; salicylic acid; dose response; enzyme activity; oxygenases; pathogenesis; messenger RNA; measurement; cytochromes; transgenic plants; gene expression; electron transfer; systemic acquired resistance
Abstract:
... Salicylic acid (SA) is a signal in systemic acquired resistance and an inducer of the alternative oxidase protein in tobacco (Nicotiana tabacum cv Xanthi nc) cell suspensions and during thermogenesis in aroid spadices. The effects of SA on the levels of alternative oxidase protein and the pathogenesis-related 1a mRNA (a marker for systemic acquired resistance), and on the partitioning of electrons ...

52. A vacuole-associated annexin protein, VCaB42, correlates with the expansion of tobacco cells

Author:
Seals, D.F.; Randall, S.K.
Source:
Plant physiology 1997 v.115 no.2 pp. 753-761
ISSN:
0032-0889
Subject:
protoplasts; morphogenesis; vacuoles; calnexin; Nicotiana tabacum; cell membranes; adenosinetriphosphatase; endoplasmic reticulum; cell suspension culture; protein composition
Abstract:
... A Ca-dependent membrane-binding protein of the annexin family, VCaB42, has previously been shown to associate with vacuolar vesicles at physiological levels of Ca. In this study we used suspension-cultured cells of tobacco (Nicotiana tabacum BY-2) to show that VCaB42 is enriched 4.5-fold in intact vacuoles, whereas evacuolated protoplasts show a 12-fold reduction in VCaB42. VCaB42 distribution is ...