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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Source 1997 v.114 no.2 Remove constraint Source: 1997 v.114 no.2
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1. ATP hydrolysis activity and polymerization state of ribulose-1,5-bisphosphate carboxylase oxygenase activase. Do the effects of Mg2+, K+, and activase concentrations indicate a functional similarity to actin?

Author:
Lilley, R.M.; Portis, A.R. Jr.
Source:
Plant physiology 1997 v.114 no.2 pp. 605-613
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; dose response; polymerization; Spinacia oleracea; adenosine triphosphate; magnesium; biochemical pathways; anions; potassium; hydrolysis; actin; enzyme activity; adenosinetriphosphatase
Abstract:
... The ATPase activity and fluorescence of ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) activase were determined over a range of MgCl2, KCl, and activase concentrations. Both salts promoted ADP release from ATP and intrinsic fluorescence enhancement by adenosine 5'-[gamma-thio] triphosphate, but Mg2+ was about 10 times more effective than K+. ATPase and fluorescence enhancement both incr ...

2. Adenylosuccinate synthetase from maize. Purification, properties, and mechanism of inhibition by 5'-phosphohydantocidin

Author:
Walters, E.W.; Lee, S.F.; Niderman, T.; Bernasconi, P.; Subramanian, M.V.; Siehl, D.L.
Source:
Plant physiology 1997 v.114 no.2 pp. 549-555
ISSN:
0032-0889
Subject:
Zea mays; succinic acid; enzyme inhibitors; ligases; pH; kinetics; enzyme activity; chemical structure; protein composition
Abstract:
... Adenylosuccinate synthetase (AdSS) is the site of action of hydantocidin, a potent microbial phytotoxin. A kinetic analysis of the mode of inhibition of a plant adenylosuccinate synthetase by the active metabolite 5'-phosphohydantocidin (5'-PH) was the objective of the present study. AdSS was purified 5800-fold from maize (Zea mays), to our knowledge the first purification of the enzyme from a pla ...
PubMed:
9193088
PubMed Central:
PMC158335

3. Allocation of S in generative growth of soybean

Author:
Sunarpi; Anderson, J.W.
Source:
Plant physiology 1997 v.114 no.2 pp. 687-693
ISSN:
0032-0889
Subject:
Glycine max; leaves; pods; seeds; sulfur; sulfates; nutrient transport; nitrogen; glutathione; cysteine; methionine; seed development; developmental stages; chemical constituents of plants
Abstract:
... Soybean plants (Glycine max L. Merr) were grown with 100 micromolar S and 15 mM N and studied with respect to S allocation during grain development. The grains accounted for 87% of the S taken up after d 42, the balance coming from internal redistribution of S from leaves and pods. Detailed studies of the leaves, pods, and grains associated with leaf axils 6 and 7 showed that sulfate accumulated i ...

4. Antagonistic but complementary actions of phytochromes A and B allow optimum seedling de-etiolation

Author:
Smith, H.; Xu, Y.; Quail, P.H.
Source:
Plant physiology 1997 v.114 no.2 pp. 637-641
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seedlings; hypocotyls; phytochrome; dose response; red light; far-red light; genetic variation; genotype; etiolation
Abstract:
... Using dichromatic radiation, we show that the actions of phytochromes A and B (phyA and phyB) in Arabidopsis thaliana are antagonistic in mediating red and far-red radiation effects on seedling de-etiolation and yet act in a complementary manner to regulate de-etiolation, irrespective of spectral composition. At low phytochrome photoequilibria inhibition of hypocotyl extension was strong, because ...

5. Brefeldin A effects in plants. Are different Golgi responses caused by different sites of action?

Author:
Staehelin, L.A.; Driouich, A.
Source:
Plant physiology 1997 v.114 no.2 pp. 401-403
ISSN:
0032-0889
Subject:
plant physiology; mycotoxins; Golgi apparatus; binding sites

6. Broad-range and binary-range acyl-acyl-carrier-protein thioesterases suggest an alternative mechanism for medium-chain production in seeds

Author:
Voelker, T.A.; Jones, A.; Cranmer, A.M.; Davies, H.M.; Knutzon, D.S.
Source:
Plant physiology 1997 v.114 no.2 pp. 669-677
ISSN:
0032-0889
Subject:
Ulmus americana; Myristica fragrans; seeds; medium chain fatty acids; biosynthesis; binding proteins; esterases; enzyme activity; complementary DNA; amino acid sequences
Abstract:
... In the current model of medium-chain (C8-14) fatty acid biosynthesis in seeds, specialized FatB acyl-acyl-carrier-protein (ACP) thioesterases are responsible for the production of medium chains. We have isolated and characterized Fat8 cDNAs from the maturing seeds of elm (Ulmus americana) and nutmeg (Myristica fragrans), which accumulate predominantly caprate (10:0)- and myristate (14:0)-containin ...
PubMed:
9193098
PubMed Central:
PMC158351

7. Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedlings

Author:
Pinhero, R.G.; Rao, M.V.; Paliyath, G.; Murr, D.P.; Fletcher, R.A.
Source:
Plant physiology 1997 v.114 no.2 pp. 695-704
ISSN:
0032-0889
Subject:
paclobutrazol; chemical reactions; seedlings; measurement; roots; glutathione; genotype; peroxidase; protein composition; leaves; Zea mays; superoxide dismutase; enzyme activity; isozymes; antioxidants; ascorbic acid; glutathione-disulfide reductase; cooling; biochemical pathways; genetic variation; cold tolerance
Abstract:
... The potential role of antioxidant enzymes in protecting maize (Zea mays L.) seedlings from chilling injury was examined by analyzing enzyme activities and isozyme profiles of chilling-susceptible (CO 316) and chilling-tolerant (CO 328) inbreds. Leaf superoxide dismutase (SOD) activity in CO 316 was nearly one-half that of CO 328, in which the high activity was maintained during the chilling and po ...

8. Characterization of the cell wall microdomain surrounding plasmodesmata in apple fruit

Author:
Roy, S.; Watada, A.E.; Wergin, W.P.
Source:
Plant physiology 1997 v.114 no.2 pp. 539-547
ISSN:
0032-0889
Subject:
Malus domestica; ripening; cytolysis; cell growth; cell division; anions; galacturonic acid; pectins; callose; antibodies; developmental stages; plasmodesmata; ultrastructure; cell wall components
Abstract:
... In fleshy fruits ripening is generally associated with a loss in tissue firmness resulting from depolymerization of wall components and separation of adjacent cells. In the regions of the wall that contain plasmodesmata, the usual sequences of ripening events, i.e. depolymerization of the middle lamellae and splitting of the walls, are not observed. In the present study we attempted to characteriz ...
Handle:
10113/31346

9. Cold acclimation and freezing tolerance. A complex interaction of light and temperature

Author:
Gary, G.R.; Chauvin, L.P.; Sarhan, F.; Huner, N.P.A.
Source:
Plant physiology 1997 v.114 no.2 pp. 467-474
ISSN:
0032-0889
Subject:
messenger RNA; dose response; redox reactions; net assimilation rate; light intensity; acclimation; Secale cereale; gene expression; transcription (genetics); frost resistance; photosynthesis; genes; temperature; plant morphology; chloroplasts
Abstract:
... By comparing growth under five different temperature and irradiance regimes (20 degrees C and 800, 250, and 50 micromoles m-2 s-1 and 5 degrees C and 250 and 50 micromoles m-2 s-1), we have examined the effects of light, temperature, and the relative reduction state of photosystem II on plant morphology, freezing tolerance (lethal temperature at which freezing injury occurs [LT50]), transcript lev ...

10. Decreased growth-induced water potential. A primary cause of growth inhibition at low water potentials

Author:
Nonami, H.; Wu, Y.; Boyer, J.S.
Source:
Plant physiology 1997 v.114 no.2 pp. 501-509
ISSN:
0032-0889
Subject:
Glycine max; cell growth; stems; developmental stages; water stress; cells; xylem; roots; hydraulic conductivity; root water potential; measurement
Abstract:
... Cell enlargement depends on a growth-induced difference in water potential to move water into the cells. Water deficits decrease this potential difference and inhibit growth. To investigate whether the decrease causes the growth inhibition, pressure wasapplied to the roots of soybean (Glycine max L. Merr.) seedlings and the growth and potential difference were monitored in the stems. In water-limi ...

11. Differential expression of the multigene family encoding the soybean mitochondrial alternative oxidase

Author:
Finnegan, P.M.; Whelan, J.; Millar, A.H.; Zhang, Q.; Smith, M.K.; Wiskich, J.T.; Day, D.A.
Source:
Plant physiology 1997 v.114 no.2 pp. 455-466
ISSN:
0032-0889
Subject:
Glycine max; protein synthesis; quantitative analysis; complementary DNA; genetic code; seedlings; cotyledons; amino acid sequences; oxidoreductases; gene expression; transcription (genetics); mitochondria; genes; enzyme activity; roots; protein composition
Abstract:
... The alternative oxidase (AOX) of the soybean (Glycine max L.) inner mitochondrial membrane isencoded by a multigene family (Aox) with three known members. Here, the Aox2 and Aox3 primary translation products, deduced from cDNA analysis, were found to be 38.1 and 36.4 kD, respectively. Direct N-terminal sequencing of partially purified AOX from cotyledons demonstrates that the mature proteins are 3 ...
PubMed:
9193084
PubMed Central:
PMC158325

12. Differential induction of lipoxygenase isoforms in wheat upon treatment with rust fungus elicitor, chitin oligosaccharides, chitosan, and methyl jasmonate

Author:
Bohland, C.; Balkenhohl, T.; Loers, G.; Feussner, I.; Grambow, H.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 679-685
ISSN:
0032-0889
Subject:
protein synthesis; dose response; chitosan; seedlings; jasmonic acid; linoleate 13S-lipoxygenase; Puccinia graminis; Triticum aestivum; leaves; plant pathogenic fungi; oligosaccharides; chitin; enzyme activity; isozymes; protein composition
Abstract:
... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...

13. Dissecting the diphenylene lodonium-sensitive NAD(P)H: quinone oxidoreductase of zucchini plasma membrane

Author:
Trost, P.; Foscarini, S.; Preger, V.; Bonora, P.; Vitale, L.; Pupillo, P.
Source:
Plant physiology 1997 v.114 no.2 pp. 737-746
ISSN:
0032-0889
Subject:
dose response; hypocotyls; NADP (coenzyme); oxidoreductases; Cucurbita pepo; enzyme inhibitors; binding sites; etiolation; plasma membrane; enzyme activity; protein composition
Abstract:
... Quinone oxidoreductase activities dependent on pyridine nucleotides are associated with the plasma membrane (PM) in zucchini (Cucurbita pepo L.) hypocotyls. In the presence of NADPH, lipophilic ubiquinone homologs with up to three isoprenoid units were reduced by intact PM vesicles with a Km of 2 to 7 micromolars. Affinities for both NADPH and NADH were similar (Km of 62 and 51 micromolars, respec ...

14. Does decrease in ribulose-1,5-bisphosphate carboxylase by antisense RbcS lead to a higher N-use efficiency of photosynthesis under conditions of saturating CO2 and light in rice plants?

Author:
Makino, A.; Shimada, T.; Takumi, S.; Kaneko, K.; Matsuoka, M.; Shimamoto, K.; Nakano, H.; Miyao-Tokutomi, M.; Mae, T.; Yamamoto, N.
Source:
Plant physiology 1997 v.114 no.2 pp. 483-491
ISSN:
0032-0889
Subject:
Oryza sativa; leaves; photosynthesis; net assimilation rate; temperature; light intensity; protein composition; nitrogen content; ribulose-bisphosphate carboxylase; enzyme activity; kinetics; nitrogen; genetic transformation; antisense DNA; genes; gene transfer; transgenic plants; carbon dioxide
Abstract:
... Rice (Oryza sativa L.) plants with decreased ribulose-1,5-bisphosphate carboxylase (Rubisco) were obtained by transformation with the rice rbcS antisense gene under the control of the rice rbcS promoter. The primary transformants were screened for the Rubisco to leaf N ratio, and the transformant with 65% wild-type Rubisco was selected as a plant set with optimal Rubisco content at saturating CO2 ...

15. Effect of water stress on cortical cell division rates within the apical meristem of primary roots of maize

Author:
Sacks, M.M.; Silk, W.K.; Burman, P.
Source:
Plant physiology 1997 v.114 no.2 pp. 519-527
ISSN:
0032-0889
Subject:
Zea mays; seedlings; roots; apical meristems; cortex; cell division; measurement; cell growth; water stress; mathematical models; equations; stress response
Abstract:
... We characterized the effect of water stress on cell division rates within the meristem of the primary root of maize (Zea mays L.) seedlings. As usual in growth kinematics, cell number density is found by counting the number of cells per small unit length of the root; growth velocity is the rate of displacement of a cellular particle found at a given distance from the apex; and the cell flux, repre ...

16. Evidence for transcriptional regulation of plastid photosynthesis genes in Arabidopsis thaliana roots

Author:
Isono, K.; Niwa, Y.; Satoh, K.; Kobayashi, H.
Source:
Plant physiology 1997 v.114 no.2 pp. 623-630
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; roots; callus; plastids; photosystem II; net assimilation rate; ribulose-bisphosphate carboxylase; enzyme activity; genetic code; genes; gene expression; chromosome mapping; transcription (genetics)
Abstract:
... Mechanisms underlying suppressed levels of transcripts for plastid photosynthesis genes in nongreen tissues such as roots and calli were analyzed in Arabidopsis thaliana, a plant suitable for further genetic dissection. A region encoding promoters of rbcL, the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, and the atpB/E operon for the beta and epsilon subunits ...
PubMed:
9193094
PubMed Central:
PMC158345

17. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

18. Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals

Author:
Smith, H.M.S.; Hicks, G.R.; Raikhel, N.V.
Source:
Plant physiology 1997 v.114 no.2 pp. 411-417
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Nicotiana tabacum; protein composition; protein transport; receptors; binding proteins; protoplasts; plant anatomy; plasma membrane
Abstract:
... Protein import into the nucleus is a two-step process. In vitro import systems from vertebrate cell extracts have shown that several soluble factors are required. One of these factors is the receptor importin alpha, which binds to nuclear localization signals (NLS) in vitro. We previously cloned an importin alpha homolog from Arabidopsis thaliana (At-IMP alpha) and demonstrated that this protein w ...
PubMed:
9193081
PubMed Central:
PMC158320

19. Influence of environmental stress on biomass partitioning in transgenic tobacco plants expressing the movement protein of tobacco mosaic virus

Author:
Balachandran, S.; Hull, R.J.; Martins, R.A.; Vaadia, Y.; Lucas, W.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 475-481
ISSN:
0032-0889
Subject:
Nicotiana tabacum; transgenic plants; gene expression; genetic vectors; Tobacco mosaic virus; viral proteins; nutrient transport; roots; shoots; water stress; nutrient deficiencies; nitrogen; potassium; phosphorus; source-sink relationships; genetic variation; translocation (plant physiology); genotype; photosynthates; stress response
Abstract:
... The influence of various environmental factors on biomass partitioning between shoots and roots in transgenic tobacco (Nicotiana tabacum) plants expressing the movement protein (MP) of tobacco mosaic virus (TMV) was investigated. TMV-MP-expressing transgenic plants exhibited a root-to-shoot ratio that was approximately 40% below that of transgenic vector control plants. When transgenic plants expr ...

20. Light regulation of the abundance of mRNA encoding a nucleolin-like protein localized in the nucleoli of pea nuclei

Author:
Tong, C.G.; Reichler, S.; Blumenthal, S.; Balk, J.; Hsieh, H.L.; Roux, S.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 643-652
ISSN:
0032-0889
Subject:
Pisum sativum; protein composition; protein synthesis; light; genetic code; complementary DNA; messenger RNA; nucleotide sequences; amino acid sequences; cell nucleolus
Abstract:
... A cDNA encoding a nucleolar protein was selected from a pea (Pisum sativum) plumule library, cloned, and sequenced. The translated sequence of the cDNA has significant percent identity to Xenopus laevis nucleolin (31%), the alfalfa (Medicago sativa) nucleolin homolog (66%), and the yeast (Saccharomyces cerevisiae) nucleolin homolog (NSR1) (28%). It also has sequence patterns in its primary structu ...
PubMed:
9193096
PubMed Central:
PMC158348