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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Subject plant pathogenic fungi Remove constraint Subject: plant pathogenic fungi
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1. 7-Dehydrobrefeldin A, a naturally occurring brefeldin A derivative, inhibits secretion and causes a cis-to-trans breakdown of Golgi stacks in plant cells

Author:
Driouich, A.; Jauneau, A.; Staehelin, L.A.
Source:
Plant physiology 1997 v.113 no.2 pp. 487-492
ISSN:
0032-0889
Subject:
Carthamus tinctorius; Alternaria carthami; plant pathogenic fungi; phytotoxins; biosynthesis; hydrolysis; esterases; enzyme activity; phytotoxicity; bioassays; Acer pseudoplatanus; cell suspension culture; Golgi apparatus; Bacillus subtilis
Abstract:
... 7-Dehydrobrefeldin A (7-oxo-BFA) is a brefeldin A (BFA) analog that, like BFA, is a potent phytotoxin of Alternaria carthami, a fungal pathogen of safflower (Carthamus tinctorius L.) plants. Both BFA and 7-oxo-BFA have been shown to be causal agents of the leaf spot disease of these plants. We have investigated the effects of 7-oxo-BFA on the secretion and the structure of the Golgi stacks of syca ...
PubMed:
9046595
PubMed Central:
PMC158164

2. Activation of host defense mechanisms by elevated production of H2O2 in transgenic plants

Author:
Wu, G.; Shortt, B.J.; Lawrence, E.B.; Leon, J.; Fitzsimmons, K.C.; Levine, E.B.; Raskin, I.; Shah, D.M.
Source:
Plant physiology 1997 v.115 no.2 pp. 427-435
ISSN:
0032-0889
Subject:
Solanum tuberosum; Verticillium dahliae; plant pathogenic fungi; leaves; pathogenicity; disease resistance; hydrogen peroxide; biosynthesis; salicylic acid; quantitative analysis; gene expression; transgenic plants; messenger RNA; genetic code; glucose oxidase; peroxidases; chitinase; isozymes; enzyme activity; stems; roots; lignin; genetic variation; chemical constituents of plants; genotype
Abstract:
... Active oxygen specieshave been postulated to perform multiple functions in plant defense, but their exact role in plant resistance to diseases is not fully understood. We have recently demonstrated H2O2-mediated disease resistance in transgenic potato (Solanum tuberosum) plants expressing a foreign gene encoding glucose oxidase. In this study we provide further evidence that the H2O2-mediated dise ...

3. Activation of plant plasma membrane Ca2+-permeable channels by race-specific fungal elicitors

Author:
Gelli, A.; Higgins, V.J.; Blumwald, E.
Source:
Plant physiology 1997 v.113 no.1 pp. 269-279
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; calcium; defense mechanisms; ion transport; plant pathogenic fungi; disease resistance; plasma membrane; phosphorylation; enzyme activity; cell suspension culture; leaves; Passalora fulva
Abstract:
... The response of plant cells to invading pathogens is regulated by fluctuations in cytosolic Ca2+ levels that are mediated by Ca2+-permeable channels located at the plasma membrane of the host cell. The mechanisms by which fungal elicitors can induce Ca2+ uptake by the host cell were examined by the application of conventional patch-clamp techniques. Whole-cell and single-channel experiments on tom ...

4. Allelic Series of Four Powdery Mildew Resistance Genes at the Pm3 Locus in Hexaploid Bread Wheat

Author:
Srichumpa, Payorm; Brunner, Susanne; Keller, Beat; Yahiaoui, Nabila
Source:
Plant physiology 2005 v.139 no.2 pp. 885-895
ISSN:
0032-0889
Subject:
cultivars; plant genetics; grain crops; haplotypes; alleles; loci; wheat; powdery mildew; Blumeria graminis f. sp. tritici; amino acid sequences; Triticum aestivum; disease resistance; molecular cloning; plant pathogenic fungi; nucleotide sequences; multigene family; hexaploidy
Abstract:
... At the Pm3 locus in hexaploid wheat (Triticum aestivum), 10 alleles conferring race-specific resistance to powdery mildew (Blumeria graminis f. sp. tritici) are known. A cluster of genes encoding coiled-coil-nucleotide-binding site-leucine-rich repeat proteins spans the Pm3 locus on wheat chromosome 1A, and one member of this gene family has recently been identified as the Pm3b resistance gene. Us ...
DOI:
10.1104/pp.105.062406
PubMed:
16183849
PubMed Central:
PMC1256003

5. Analysis of Two in Planta Expressed LysM Effector Homologs from the Fungus Mycosphaerella graminicola Reveals Novel Functional Properties and Varying Contributions to Virulence on Wheat

Author:
Marshall, Rosalind; Kombrink, Anja; Motteram, Juliet; Loza-Reyes, Elisa; Lucas, John; Hammond-Kosack, Kim E.; Thomma, Bart P.H.J.; Rudd, Jason J.
Source:
Plant physiology 2011 v.156 no.2 pp. 756-769
ISSN:
0032-0889
Subject:
protein binding; tomatoes; Mycosphaerella graminicola; sporulation; Solanum lycopersicum; enzymes; hosts; plant proteins; hyphae; wheat; amino acids; Triticum aestivum; genes; plant pathogenic fungi; functional properties; virulence; molds (fungi); leaves; Passalora fulva
Abstract:
... Secreted effector proteins enable plant pathogenic fungi to manipulate host defenses for successful infection. Mycosphaerella graminicola causes Septoria tritici blotch disease of wheat (Triticum aestivum) leaves. Leaf infection involves a long (approximately 7 d) period of symptomless intercellular colonization prior to the appearance of necrotic disease lesions. Therefore, M. graminicola is cons ...
DOI:
10.1104/pp.111.176347
PubMed:
21467214
PubMed Central:
PMC3177273

6. Analysis of nitric oxide signaling functions in tobacco cells challenged by the elicitor cryptogein

Author:
Lamotte, O.; Gould, K.; Lecourieux, D.; Sequeira-Legrand, A.; Lebrun-Garcia, A.; Durner, J.; Pugin, A.; Wendehenne, D.
Source:
Plant physiology 2004 v.135 no.1 pp. 516-529
ISSN:
0032-0889
Subject:
Nicotiana tabacum; tobacco; cell suspension culture; cultured cells; nitric oxide; signal transduction; calcium; host-pathogen relationships; Phytophthora cryptogea; plant pathogenic fungi; fungal proteins
Abstract:
... Nitric oxide (NO) has recently emerged as an important cellular mediator in plant defense responses. However, elucidation of the biochemical mechanisms by which NO participates in this signaling pathway is still in its infancy. We previously demonstrated that cryptogein, an elicitor of tobacco defense responses, triggers a NO burst within minutes in epidermal sections from tobacco leaves (Nicotian ...
DOI:
10.1104/pp.104.038968
PubMed:
15122020
PubMed Central:
PMC429403

7. Arabidopsis downy mildew resistance gene RPP27 encodes a receptor-like protein similar to CLAVATA2 and tomato Cf-9

Author:
Tor, M.; Brown, D.; Cooper, A.; Woods-Tor, A.; Sjolander, K.; Jones, J.D.G.; Holub, E.B.
Source:
Plant physiology 2004 v.135 no.2 pp. 1100-1112
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transgenic plants; plant proteins; receptors; complementary DNA; sequence analysis; messenger RNA; chromosome mapping; disease resistance; resistance mechanisms; downy mildew; Peronospora parasitica; plant pathogenic fungi; Solanum lycopersicum var. lycopersicum; tomatoes; amino acid sequences
Abstract:
... The Arabidopsis Ler-RPP27 gene confers AtSgt1b-independent resistance to downy mildew (Peronospora parasitica) isolate Hiks1. The RPP27 locus was mapped to a four-bacterial artificial chromosome interval on chromosome 1 from genetic analysis of a cross between the enhanced susceptibility mutant Col-edm1 (Col-sgt1) and Landsberg erecta (Ler-0). A Cf-like candidate gene in this interval was PCR ampl ...
DOI:
10.1104/pp.103.037770
PubMed:
15155873
PubMed Central:
PMC514143

8. An Arabidopsis mutant defective in jasmonate response is allelic to the auxin-signaling mutant axr1

Author:
Tiryaki, I.; Staswick, P.E.
Source:
Plant physiology 2002 v.130 no.2 pp. 887-894
ISSN:
0032-0889
Subject:
seedling growth; alleles; seedlings; benzyladenine; salicylic acid; plant pathogenic fungi; roots; plant diseases and disorders; jasmonic acid; gene expression; biological resistance; brassinolide; Arabidopsis thaliana; gallic acid; host plants; linoleate 13S-lipoxygenase; methyl jasmonate; ferulic acid; indole acetic acid; 1-aminocyclopropane-1-carboxylic acid; acid phosphatase; Pythium irregulare; mutants; nucleotide sequences; abscisic acid
Abstract:
... A screen for Arabidopsis mutants that were insensitive to methyl jasmonate (MeJA) in an assay for seedling root growth yielded only alleles of previously isolated mutants jar1 and coi1, with one exception. Mapping of the locus and morphological characterization of the new mutant suggested it might be allelic to axr1, which had not previously been reported to show resistance to MeJA. The F1 from a ...
PubMed:
12376653
PubMed Central:
PMC166615

9. An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins

Author:
Epple, P.; Apel, K.; Bohlmann, H.
Source:
Plant physiology 1995 v.109 no.3 pp. 813-820
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; sequence analysis; plant development; transcriptional activation; complementary DNA; ecotypes; plant pathology; spatial distribution; amino acid sequences; gene expression; genes; nucleotide sequences; plant pathogenic fungi; genomics; RNA; defense mechanisms
Abstract:
... Two cDNAs encoding thionin preproproteins have been isolated fromArabidopsis thaliana. The corresponding genes have been designated Thi2.1 and Thi2.2 Southern blot analysis suggests that A. thaliana most probably contains single genes for both thionins. Thi2.2 transcripts have a low basal level in seedlings and show circadian variation. Thi2.2 transcripts were also detected in rosette leaves. No p ...
PubMed:
8552715
PubMed Central:
PMC161381

10. The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia

Author:
Anderson, Jonathan P.; Lichtenzveig, Judith; Gleason, Cynthia; Oliver, Richard P.; Singh, Karam B.
Source:
Plant physiology 2010 v.154 no.2 pp. 861-873
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Medicago truncatula; Phytophthora medicaginis; Thanatephorus cucumeris; adverse effects; canola; disease resistance; ethylene; gene overexpression; genes; genetic resistance; germplasm screening; grain crops; legumes; nodulation; phenotype; plant development; plant pathogenic fungi; promoter regions; response elements; root-knot nematodes; roots; symbiosis; transcription factors
Abstract:
... The fungal necrotrophic pathogen Rhizoctonia solani is a significant constraint to a range of crops as diverse as cereals, canola, and legumes. Despite wide-ranging germplasm screens in many of these crops, no strong genetic resistance has been identified, suggesting that alternative strategies to improve resistance are required. In this study, we characterize moderate resistance to R. solani anas ...
DOI:
10.1104/pp.110.163949
PubMed:
20713618
PubMed Central:
PMC2949043

11. Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery

Author:
Miklis, Marco; Consonni, Chiara; Bhat, Riyaz A.; Lipka, Volker; Schulze-Lefert, Paul; Panstruga, Ralph
Source:
Plant physiology 2007 v.144 no.2 pp. 1132-1143
ISSN:
0032-0889
Subject:
Phytoplasma; plant development; barley; Erysiphe pisi; Blumeria graminis; Hordeum vulgare; cell walls; microfilaments; powdery mildew; plant pathogenic fungi; disease resistance; immune response
Abstract:
... Cell polarization is a crucial process during plant development, as well as in plant-microbe interactions, and is frequently associated with extensive cytoskeletal rearrangements. In interactions of plants with inappropriate fungal pathogens (so-called non-host interactions), the actin cytoskeleton is thought to contribute to the establishment of effective barriers at the cell periphery against fu ...
DOI:
10.1104/pp.107.098897
PubMed:
17449647
PubMed Central:
PMC1914182

12. Benzothiadiazole-mediated induced resistance to Fusarium oxysporum f.sp. radicis-lycopersici in tomato

Author:
Benhamou, N.; Belanger, R.R.
Source:
Plant physiology 1998 v.118 no.4 pp. 1203-1212
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; Fusarium oxysporum f. sp. radicis-lycopersici; plant pathogenic fungi; disease resistance; azoles; roots; pathogenesis; developmental stages; pathogenicity
Abstract:
... Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH), a synthetic chemical, was applied as a foliar spray to tomato (Lycopersicon esculentum) plants and evaluated for its potential to confer increased resistance against the soil-borne pathogen Fusarium oxysporum f. sp. radicis-lycopersici (FORL). In nontreated tomato plants all root tissues were massively colonized by FORL hyphae. Pat ...

13. Biosynthesis of camalexin from tryptophan pathway intermediates in cell-suspension cultures of Arabidopsis

Author:
Zook, M.
Source:
Plant physiology 1998 v.118 no.4 pp. 1389-1393
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; biosynthesis; tryptophan; biochemical pathways; plant pathogenic fungi; disease resistance; Bipolaris zeicola; cell suspension culture; phytoalexins
Abstract:
... Camalexin (3-thiazol-2'-yl-indole) is the principal phytoalexin that accumulates in Arabidopsis after infection by fungi or bacteria. Camalexin accumulation was detectable in Arabidopsis cell-suspension cultures 3 to 5 h after inoculation with Cochliobolus carbonum (Race 1), and then increased rapidly from 7 to 24 h after inoculation. Levels of radioactivity incorporated into camalexin during a 1. ...
PubMed:
9847113
PubMed Central:
PMC34755

14. Biphasic Gene Expression Changes Elicited by Phakopsora pachyrhizi in Soybean Correlate with Fungal Penetration and Haustoria Formation

Author:
Katherine T. Schneider; Martijn van de Mortel; Timothy J. Bancroft; Edward Braun; Dan Nettleton; Rex T. Nelson; Reid D. Frederick; Thomas J. Baum; Michelle A. Graham; Steven A. Whitham
Source:
Plant physiology 2011 v.157 no.1 pp. 355-371
ISSN:
0032-0889
Subject:
rust diseases; correlation; Glycine max; growth and development; host-pathogen relationships; host plants; soybeans; Phakopsora pachyrhizi; gene expression; resistance mechanisms; plant pathogenic fungi; plant response; disease resistance; genes; virulence; infection
Abstract:
... Inoculation of soybean (Glycine max) plants with Phakopsora pachyrhizi, the causal organism of Asian soybean rust, elicits a biphasic response characterized by a burst of differential gene expression in the first 12 h. A quiescent period occurs from 24 to 48 h after inoculation, in which P. pachyrhizi continues to develop but does not elicit strong host responses, followed by a second phase of int ...
Handle:
10113/50378
DOI:
10.1104/pp.111.181149
PubMed:
21791600
PubMed Central:
PMC3165884

15. Cell-specific production and antimicrobial activity of naphthoquinones in roots of Lithospermum erythrorhizon

Author:
Brigham, L.A.; Michaels, P.J.; Flores, H.E.
Source:
Plant physiology 1999 v.119 no.2 pp. 417-428
ISSN:
0032-0889
Subject:
soil fungi; biosynthesis; soil bacteria; immunohistochemistry; hyphae; culture media; Rhizobium rhizogenes; root hairs; plant pathogenic fungi; chemical constituents of plants; Lithospermum erythrorhizon; tissue culture
Abstract:
... Pigmented naphthoquinone derivatives of shikonin are produced at specific times and in specific cells of Lithospermum erythrorhizon roots. Normal pigment development is limited to root hairs and root border cells in hairy roots grown on "noninducing" medium, whereas induction of additional pigment production by abiotic (CuSO4) or biotic (fungal elicitor) factors increases the amount of total pigme ...

16. Cercospora beticola toxin. X. Inhibition of plasma membrane H+-ATPase by beticolin-1

Author:
Simon-Plas, F.; Gomes, E.; Milat, M.L.; Pugin, A.; Blein, J.P.
Source:
Plant physiology 1996 v.111 no.3 pp. 773-779
ISSN:
0032-0889
Subject:
Cercospora beticola; plant pathogenic fungi; mycotoxins; adenosinetriphosphatase; enzyme activity; enzyme inhibitors; kinetics; proton pump; phosphorylation; metabolites; plasma membrane
Abstract:
... Beticolin-1 is a toxin produced by the fungus Cercospora beticola. The chemical structure of this toxin was previously elucidated. The effects of beticolin-1 on purified corn root plasma membrane H+-ATPase were studied in a solubilized form or were reconstituted intoliposome membranes. The ATP hydrolysis activity of the purified solubilized enzyme was inhibited by micromolar concentrations of beti ...

17. Characterization and expression of caffeoyl-coenzyme A 3-O-methyltransferase proposed for the induced resistance response of Vitis vinifera L

Author:
Busam, G.; Junghanns, K.T.; Kneusel, R.E.; Kassemeyer, H.H.; Matern, U.
Source:
Plant physiology 1997 v.115 no.3 pp. 1039-1048
ISSN:
0032-0889
Subject:
lignification; plant pathogenic fungi; salicylic acid; genes; phenotype; methylation; systemic acquired resistance; clones; gene expression; disease resistance; esters; Vitis vinifera; complementary DNA; amino acid sequences; pathogenicity; enzyme activity; caffeic acid; coenzyme A; niacin; transferases; cell suspension culture; chitinase
Abstract:
... Cell-suspension cultures of Vitis vinifera L. cv Pinot Noir accumulated resveratrol upon fungal elicitation, and the activity of S-adenosyl-1-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT), yielding feruloyl-CoA, increased to a transient maximum at 12 to 15 h. CCoAOMT cDNA was cloned from the elicited cells and was shown to encode a polypeptide highly homologous to CCoAOMTs f ...
PubMed:
9390437
PubMed Central:
PMC158567

18. Characterization of GaWRKY1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-delta-cadinene synthase-A

Author:
Xu, Y.H.; Wang, J.W.; Wang, S.; Wang, J.Y.; Chen, X.Y.
Source:
Plant physiology 2004 v.135 no.1 pp. 507-515
ISSN:
0032-0889
Subject:
sequence alignment; fiber crops; plant pathogenic fungi; promoter regions; plant proteins; Gossypium hirsutum; Nicotiana tabacum; gene expression regulation; transcription factors; complementary DNA; amino acid sequences; isomerases; tobacco; Gossypium arboreum; cotton; transgenes; host-pathogen relationships; transgenic plants; Saccharomyces cerevisiae; DNA-binding proteins; Verticillium dahliae
Abstract:
... The cotton (+)-delta-cadinene synthase (CAD1), a sesquiterpene cyclase, catalyzes a branch-point step leading to biosynthesis of sesquiterpene phytoalexins, including gossypol. CAD1-A is a member of CAD1 gene family, and its promoter contains a W-box palindrome with two reversely oriented TGAC repeats, which are the proposed binding sites of WRKY transcription factors. We isolated several WRKY cDN ...
DOI:
10.1104/pp.104.038612
PubMed:
15133151
PubMed Central:
PMC429402

19. Characterization of a diffusible signal capable of inducing defense gene expression in tobacco

Author:
Chappell, J.; Levine, A.; Tenhaken, R.; Lusso, M.; Lamb, C.
Source:
Plant physiology 1997 v.113 no.2 pp. 621-629
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; Phytophthora cryptogea; plant pathogenic fungi; pathogenicity; disease resistance; genes; phytoalexins; biosynthesis; lyases; enzyme activity; measurement; gene expression; Glycine max; hydrogen peroxide; pathogenesis-related proteins; sesquiterpenoids
Abstract:
... Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynthetic enzyme in solanaceous plants) when added to fresh cell-suspension cultures. ...

20. Characterization of a new antifungal chitin-binding peptide from sugar beet leaves

Author:
Nielsen, K.K.; Nielsen, J.E.; Madrid, S.M.; Mikkelsen, J.D.
Source:
Plant physiology 1997 v.113 no.1 pp. 83-91
ISSN:
0032-0889
Subject:
plant pathogenic fungi; binding proteins; gene expression; disease resistance; cysteine; leaves; antifungal properties; protein composition; messenger RNA; complementary DNA; genetic code; amino acid sequences; pathogenicity; hevein; glycine (amino acid); Beta vulgaris; chitin; Cercospora beticola; flowers; physicochemical properties; nucleotide sequences; niacin; chitinase
Abstract:
... The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residue sequence of IWF4 is rich in cysteines (6) and glycines (7) and has a highly bas ...
PubMed:
9008390
PubMed Central:
PMC158118

21. Characterization of an Isoflavonoid-Specific Prenyltransferase from Lupinus albus

Author:
Guoan Shen; David Huhman; Zhentian Lei; John Snyder; Lloyd W. Sumner; Richard A. Dixon
Source:
Plant physiology 2012 v.159 no.1 pp. 70-80
ISSN:
0032-0889
Subject:
antimicrobial properties; engineering; transmembrane proteins; Lupinus albus; enzymes; amino acid sequences; genistein; genes; plant pathogenic fungi; legumes; anti-infective agents; Medicago truncatula; roots
Abstract:
... Prenylated flavonoids and isoflavonoids possess antimicrobial activity against fungal pathogens of plants. However, only a few plant flavonoid and isoflavonoid prenyltransferase genes have been identified to date. In this study, an isoflavonoid prenyltransferase gene, designated as LaPT1, was identified from white lupin (Lupinus albus). The deduced protein sequence of LaPT1 shared high homologies ...
DOI:
10.1104/pp.112.195271
PubMed:
22430842
PubMed Central:
PMC3375986

22. Characterization of the complex locus of bean encoding polygalacturonase-inhibiting proteins reveals subfunctionalization for defense against fungi and insects

Author:
D'Ovidio, R.; Raiola, A.; Capodicasa, C.; Devoto, A.; Pontiggia, D.; Roberti, S.; Galletti, R.; Conti, E.; O'Sullivan, D.; De Lorenzo, G.
Source:
Plant physiology 2004 v.135 no.4 pp. 2424-2435
ISSN:
0032-0889
Subject:
plant damage; beans; phytophagous insects; Lygus rugulipennis; polygalacturonase; enzyme inhibition; mutation; Fusarium fujikuroi; Phaseolus vulgaris; enzyme inhibitors; amino acid sequences; resistance mechanisms; plant pathogenic fungi; mechanical damage; nucleotide sequences; genetic variation; gene expression regulation; Adelphocoris lineolatus; plant proteins
Abstract:
... Polygalacturonase-inhibiting proteins (PGIPs) are extracellular plant inhibitors of fungal endopolygalacturonases (PGs) that belong to the superfamily of Leu-rich repeat proteins. We have characterized the full complement of pgip genes in the bean (Phaseolus vulgaris) genotype BAT93. This comprises four clustered members that span a 50-kb region and, based on their similarity, form two pairs (Pvpg ...
DOI:
10.1104/pp.104.044644
PubMed:
15299124
PubMed Central:
PMC520809

23. Characterization of two novel type I ribosome-inactivating proteins from the storage roots of the Andean crop Mirabilis expansa

Author:
Vivanco, J.M.; Savary, B.J.; Flores, H.E.
Source:
Plant physiology 1999 v.119 no.4 pp. 1447-1456
ISSN:
0032-0889
Subject:
Mirabilis; ribosomes; roots; tubers; molecular weight; isoelectric point; pH; amino acid sequences; protein composition; molecular conformation; antifungal properties; antibacterial properties; ribosomal RNA; hyphae; plant pathogenic bacteria; plant pathogenic fungi; antiviral properties
Abstract:
... Two novel type I ribosome-inactivating proteins (RIPs) were found in the storage roots of Mirabilis expansa, an underutilized Andean root crop. The two RIPs, named ME1 and ME2, were purified to homogeneity by ammonium sulfate precipitation, cation-exchange perfusion chromatography, and C(4) reverse-phase chromatography. The two proteins were found to be similar in size (27 and 27.5 kD) by sodium d ...
PubMed:
10198104
PubMed Central:
PMC32030

24. Chlorophyll fluorescence induction in leaves of Phaseolus vulgaris infected with bean rust (Uromyces appendiculatus)

Author:
Peterson, R.B.; Aylor, D.E.
Source:
Plant physiology 1995 v.108 no.1 pp. 163-171
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Uromyces appendiculatus; plant pathogenic fungi; pathogenesis; developmental stages; chlorosis; leaves; chlorophyll; fluorescence; photosystem II; image analysis; plant morphology; pathogenicity; light harvesting complex
Abstract:
... To our knowledge, this report describes the first application of video imaging of Chl fluorescence to the study of light utilization in photosystem II of attached leaves of Phaseolus vulgaris infected with the obligate biotrophic fungus Uromyces appendiculatus (race 38). The video-based detection system produced a spatially resolved, quantifiable signal that was highly specific for chlorophyll flu ...

25. A Circadian Rhythm-Regulated Tomato Gene Is Induced by Arachidonic Acid and Phythophthora infestans Infection

Author:
Weyman, Philip D.; Pan, Zhiqiang; Feng, Qin; Gilchrist, David G.; Bostock, Richard M.
Source:
Plant physiology 2006 v.140 no.1 pp. 235-248
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; tomatoes; apoptosis; plant proteins; complementary DNA; sequence analysis; promoter regions; transcription (genetics); gene expression regulation; circadian rhythm; arachidonic acid; Phytophthora infestans; plant pathogenic fungi; proteinase inhibitors; alpha-amylase; storage proteins; signal transduction; genome; plasma membrane; amino acid sequences
Abstract:
... A cDNA clone of unknown function, DEA1, was isolated from arachidonic acid-treated tomato (Solanum lycopersicum) leaves by differential display PCR. The gene, DEA1, is expressed in response to the programmed cell death-inducing arachidonic acid within 8 h following treatment of a tomato leaflet, 16 h prior to the development of visible cell death. DEA1 transcript levels were also affected by the l ...
Handle:
10113/7292
DOI:
10.1104/pp.105.068874
PubMed:
16361525
PubMed Central:
PMC1326047

26. Comparative analyses of potato expressed sequence tag libraries

Author:
Ronning, C.M.; Stegalkina, S.S.; Ascenzi, R.A.; Bougri, O.; Hart, A.L.; Utterbach, T.R.; Vanaken, S.E.; Riedmuller, S.B.; White, J.A.; Cho, J.
Source:
Plant physiology 2003 v.131 no.2 pp. 419-429
ISSN:
0032-0889
Subject:
developmental stages; Solanum tuberosum; plant extracts; potatoes; plant pathogenic fungi; tomatoes; seed dormancy; plant diseases and disorders; blight; gene expression; transcriptome; seed tubers; multigene family; messenger RNA; DNA libraries; host plants; Phytophthora infestans; transcription (genetics); Solanum lycopersicum var. lycopersicum; plant tissues; consensus sequence; expressed sequence tags; tuber sprouting; chemical constituents of plants
Abstract:
... The cultivated potato (Solanum tuberosum) shares similar biology with other members of the Solanaceae, yet has features unique within the family, such as modified stems (stolons) that develop into edible tubers. To better understand potato biology, we have undertaken a survey of the potato transcriptome using expressed sequence tags (ESTs) from diverse tissues. A total of 61,940 ESTs were generate ...
Handle:
10113/26561
DOI:
10.1104/pp.013581
PubMed:
12586867
PubMed Central:
PMC166819

27. Comparative biochemistry of the oxidative burst produced by rose and French bean cells reveals two distinct mechanisms

Author:
Bolwell, G.P.; Davies, D.R.; Gerrish, C.; Auh, C.K.; Murphy, T.M.
Source:
Plant physiology 1998 v.116 no.4 pp. 1379-1385
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Colletotrichum lindemuthianum; plant pathogenic fungi; cell walls; hydrogen peroxide; disease resistance; oxidation; plasma membrane; peroxidase; quantitative analysis; Phytophthora; Armoracia rusticana; enzyme activity; Rosa damascena; biosynthesis; NADP (coenzyme); cultured cells; alkalinity; chemical constituents of plants
Abstract:
... Cultured cells of rose (Rosa damascena) treated with an elicitor derived from Phytophthora spp. and suspension-cultured cells of French bean (Phaseolus vulgaris) treated with an elicitor derived from the cell walls of Colletotrichum lindemuthianum both produced H2O2. It has been hypothesized that in rose cells H2O2 is produced by a plasma membrane NAD(P)H oxidase (superoxide synthase), whereas in ...

28. Comparison of binding properties and early biological effects of elicitins in tobacco cells

Author:
Bourque, S.; Ponchet, M.; Binet, M.N.; Ricci, P.; Pugin, A.; Lebrun-Garcia, A.
Source:
Plant physiology 1998 v.118 no.4 pp. 1317-1326
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; protein composition; Phytophthora; plant pathogenic fungi; disease resistance; defense mechanisms; calcium; ion transport; alkalinity; plasma membrane; binding sites
Abstract:
... Elicitins are a family of small proteins secreted by Phytophthora species that have a high degree of homology and elicit defense reactions in tobacco (Nicotiana tabacum). They display acidic or basic characteristics, the acidic elicitins being less efficient in inducing plant necrosis. In this study we compared the binding properties of four elicitins (two basic and two acidic) and early-induced s ...

29. Comparison of the ability of partially N-acetylated chitosan and chitooligosaccharides to elicit resistance reactions in wheat leaves

Author:
Vander, P.; Varum, K.M.; Domard, A.; El-Gueddari, N.E.; Moerschbacher, B.M.
Source:
Plant physiology 1998 v.118 no.4 pp. 1353-1359
ISSN:
0032-0889
Subject:
Triticum aestivum; leaves; plant pathogenic fungi; pathogenicity; disease resistance; chitosan; dose response; oligosaccharides; lignin; biosynthesis; polymerization
Abstract:
... Chitin, a linear polysaccharide composed of (1 leads to 4)-linked 2-acetamido-2-deoxy-beta-D-glucopyranose (GlcNAc) residues, and chitosan, the fully or partially N-acetylated, water-soluble derivative of chitin composed of (1 leads to 4)-linked GlcNAc and 2-amino-2-deoxy-beta-D-glucopyranose (GlcN), have been proposed as elicitors of defense reactions in higher plants. We tested and compared the ...

30. Complex formation of myrosinase isoenzymes in oilseed rape seeds are dependent on the presence of myrosinase-binding proteins

Author:
Eriksson, S.; Andreasson, E.; Ekbom, B.; Graner, G.; Pontoppidan, B.; Taipalensuu, J.; Zhang, J.; Rask, L.; Meijer, J.
Source:
Plant physiology 2002 v.129 no.4 pp. 1592-1599
ISSN:
0032-0889
Subject:
antisense DNA; plant extracts; Alternaria brassicae; plant pathogenic fungi; Brassica napus; thioglucosidase; chemical degradation; plant proteins; plant pests; binding proteins; Tenebrio molitor; glycoproteins; insect pests; seeds; Plenodomus lingam; host plants; bioassays; feeding behavior; enzyme activity; isozymes; fractionation; biosynthesis; transgenic plants; myosin; nucleotide sequences; chemical constituents of plants
Abstract:
... The enzyme myrosinase (EC 3.2.3.1) degrades the secondary compounds glucosinolates upon wounding and serves as a defense to generalist pests in Capparales. Certain myrosinases are present in complexes together with other proteins such as myrosinase-binding proteins (MBP) in extracts of oilseed rape (Brassica napus) seeds. Immunhistochemical analysis of wild-type seeds showed that MBPs were present ...
PubMed:
12177471
PubMed Central:
PMC166746

31. Coordinate accumulation of antifungal proteins and hexoses constitutes a developmentally controlled defense response during fruit ripening in grape

Author:
Salzman, R.A.; Tikhonova, I.; Bordelon, B.P.; Hasegawa, P.M.; Bressan, R.A.
Source:
Plant physiology 1998 v.117 no.2 pp. 465-472
ISSN:
0032-0889
Subject:
developmental stages; plant pathogenic fungi; Guignardia bidwellii; Daucus carota; disease resistance; Vitis vinifera; hexoses; protein composition; antifungal properties; Zea mays; quantitative analysis; Vitis labrusca; amino acid sequences; enzyme activity; Solanum lycopersicum var. lycopersicum; protein synthesis; biosynthesis; Spinacia oleracea; ripening; chitinase
Abstract:
... During ripening of grape (Vitis labruscana L. cv Concord) berries, abundance of several proteins increased, coordinately with hexoses, to the extent that these became the predominant proteins in the ovary. These proteins have been identified by N-terminal amino acid-sequence analysis and/or function to be a thaumatin-like protein (grape osmotin), a lipid-transfer protein, and a basic and an acidic ...
PubMed:
9625699
PubMed Central:
PMC34966

32. Coordinated regulation of genes for secretion in tobacco at late developmental stages: association with resistance against oomycetes

Author:
Hugot, K.; Riviere, M.P.; Moreilhon, C.; Dayem, M.A.; Cozzitorto, J.; Arbiol, G.; Barbry, P.; Weiss, C.; Galiana, E.
Source:
Plant physiology 2004 v.134 no.2 pp. 858-870
ISSN:
0032-0889
Subject:
complementary DNA; host-pathogen relationships; systemic acquired resistance; fungal diseases of plants; developmental stages; signal transduction; transcription (genetics); Nicotiana tabacum; signal peptide; salicylic acid; disease resistance; Phytophthora nicotianae var. parasitica; plant pathogenic fungi; pathogenesis-related proteins; gene expression regulation; nucleotide sequences; tobacco
Abstract:
... Besides the systemic acquired resistance (SAR) induced in response to microbial stimulation, host plants may also acquire resistance to pathogens in response to endogenous stimuli associated with their own development. In tobacco (Nicotiana tabacum), the vegetative-to-flowering transition comes along with a susceptibility-to-resistance transition to the causal agent of black shank disease, the oom ...
DOI:
10.1104/pp.103.034173
PubMed:
14764907
PubMed Central:
PMC344560

33. Correlation between binding affinity and necrosis-inducing activity of mutant AVR9 peptide elicitors

Author:
Kooman-Gersmann, M.; Vogelsang, R.; Vossen, P.; Hooven, H.W. van den.; Mahe, E.; Honee, G.; Wit, P.J.G.M. de.
Source:
Plant physiology 1998 v.117 no.2 pp. 609-618
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; plasma membrane; glucosamine; genetic vectors; necrosis; Potato virus X; peptides; gene expression; plant pathogenic fungi; disease resistance; mutants; genes; genetic variation; Nicotiana clevelandii; genotype; binding sites; virulence; Passalora fulva
Abstract:
... The race-specific peptide elicitor AVR9 of the fungus Cladosporium fulvum induces a hypersensitive response only in tomato (Lycopersicon esculentum) plants carrying the complementary resistance gene Cf-9 (MoneyMaker-Cf9). A binding site for AVR9 is present on the plasma membranes of both resistant and susceptible tomato genotypes. We used mutant AVR9 peptides to determine the relationship between ...
PubMed:
9625714
PubMed Central:
PMC34981

34. Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression

Author:
Chao Li; Xin He; Xiangyin Luo; Li Xu; Linlin Liu; Ling Min; Li Jin; Longfu Zhu; Xianlong Zhang
Source:
Plant physiology 2014 v.166 no.4 pp. 2179-2194
ISSN:
0032-0889
Subject:
Botrytis cinerea; Gossypium barbadense; Gossypium hirsutum; Verticillium dahliae; binding sites; cotton; flowering; fungal diseases of plants; gel electrophoresis; gene expression regulation; gene overexpression; gibberellins; immune response; jasmonic acid; methyl jasmonate; phenotype; plant pathogenic fungi; plant response; proteins; signal transduction; transcription (genetics)
Abstract:
... Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the ...
DOI:
10.1104/pp.114.246694
PubMed:
25301887
PubMed Central:
PMC4256851

35. Cucumber hypocotyls respond to cutin monomers via both an inducible and a constitutive H2O2-generating system

Author:
Kauss, H.; Fauth, M.; Merten, A.; Jeblick, W.
Source:
Plant physiology 1999 v.120 no.4 pp. 1175-1182
ISSN:
0032-0889
Subject:
cutin; Colletotrichum orbiculare; protein synthesis; hypocotyls; Cucumis sativus; seedlings; hydrogen peroxide; spore germination; mechanical damage; fungal spores; plant pathogenic fungi; etiolation; induced resistance; oligosaccharides; defense mechanisms
Abstract:
... Hypocotyls from etiolated cucumber (Cucumis sativa L.) seedlings were gently abraded at their surface to allow permeation of elicitors. Segments from freshly abraded hypocotyls were only barely competent for H2O2 elicitation with fungal elicitor or hydroxy fatty acids (classical cutin monomers). However, elicitation competence developed subsequent to abrasion, reaching an optimum after about 4 h. ...

36. Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway

Author:
Scofield, S.R.; Huang, L.; Brandt, A.S.; Gill, B.S.
Source:
Plant physiology 2005 v.138 no.4 pp. 2165-2173
ISSN:
0032-0889
Subject:
Triticum aestivum; wheat; hexaploidy; Hordeum vulgare; barley; messenger RNA; gene silencing; plant genetics; rust diseases; disease resistance; resistance mechanisms; Barley stripe mosaic virus; genetic vectors; Puccinia recondita; plant pathogenic fungi; nucleotide sequences
Abstract:
... Virus-induced gene silencing (VIGS) is an important tool for the analysis of gene function in plants. In VIGS, viruses engineered to carry sequences derived from plant gene transcripts activate the host's sequence-specific RNA degradation system. This mechanism targets the RNAs of the viral genome for degradation, and as the virus contains transcribed plant sequence, homologous host mRNAs are also ...
Handle:
10113/7280
DOI:
10.1104/pp.105.061861
PubMed:
16024691
PubMed Central:
PMC1183404

37. Differences in Cell Death Induction by Phytophthora Elicitins Are Determined by Signal Components Downstream of MAP Kinase Kinase in Different Species of Nicotiana and Cultivars of Brassica rapa and Raphanus sativus

Author:
Takemoto, Daigo; Hardham, Adrienne R.; Jones, David A.
Source:
Plant physiology 2005 v.138 no.3 pp. 1491-1504
ISSN:
0032-0889
Subject:
plant biochemistry; radishes; signal transduction; plant pathogenic fungi; Phytophthora cryptogea; Phytophthora sojae; vegetable crops; mycotoxins; Nicotiana tabacum; Phytophthora megasperma; gene expression regulation; apoptosis; Arabidopsis thaliana; transcription factors; cultivars; Brassica nigra; Phytophthora cinnamomi; Alternaria brassicicola; kale; pathogenesis-related proteins; Brassica oleracea; plant physiology; hypersensitive response; tobacco; pathophysiology; plant genetics; protein kinases; host-pathogen relationships; transgenic plants; Phytophthora nicotianae; Raphanus sativus; DNA-binding proteins; Brassica rapa; genetic variation; fungal proteins
Abstract:
... Elicitins are small, secreted proteins produced by species of the plant-pathogenic oomycete Phytophthora. They induce hypersensitive cell death in most Nicotiana species and in some cultivars of Brassica rapa and Raphanus sativus. In this study, two true-breeding Fast Cycling B. rapa lines were established that showed severe necrosis (line 7-R) or no visible response (line 18-NR) after treatment w ...
DOI:
10.1104/pp.104.058388
PubMed:
15980203
PubMed Central:
PMC1176420

38. Differential antifungal and calcium channel-blocking activity among structurally related plant defensins

Author:
Spelbrink, R.G.; Dilmac, N.; Allen, A.; Smith, T.J.; Shah, D.M.; Hockerman, G.H.
Source:
Plant physiology 2004 v.135 no.4 pp. 2055-2067
ISSN:
0032-0889
Subject:
Fusarium graminearum; forage crops; molecular models; Neurospora crassa; Komagataella pastoris; arginine; calcium; radishes; Medicago sativa; antifungal properties; calcium channel blockers; alfalfa; antifungal proteins; amino acid sequences; plant pathogenic fungi; Raphanus sativus; structure-activity relationships; Medicago truncatula; plant proteins
Abstract:
... Plant defensins are a family of small Cys-rich antifungal proteins that play important roles in plant defense against invading fungi. Structures of several plant defensins share a Cys-stabilized α/β-motif. Structural determinants in plant defensins that govern their antifungal activity and the mechanisms by which they inhibit fungal growth remain unclear. Alfalfa (Medicago sativa) seed defensin, M ...
DOI:
10.1104/pp.104.040873
PubMed:
15299136
PubMed Central:
PMC520777

39. Differential expression of chitinases in Vitis vinifera L. responding to systemic acquired resistance activators or fungal challenge

Author:
Busam, G.; Kassemeyer, H.H.; Matern, U.
Source:
Plant physiology 1997 v.115 no.3 pp. 1029-1038
ISSN:
0032-0889
Subject:
Plasmopara viticola; messenger RNA; protein synthesis; complementary DNA; Vitis rupestris; yeasts; clones; gene expression; pathogenicity; amino acid sequences; plant pathogenic fungi; disease resistance; salicylic acid; genes; Vitis vinifera; transcription (genetics); leaves; chitinase
Abstract:
... The concept of systemic acquired resistance (SAR) enablesa novel approach to crop protection, and particular pathogenesis-related proteins, i.e. an acidic chitinase, have been classified as markers of the SAR response. Basic class I (VCHIT1b) and a class III (VCH3) chitinase cDNAs were cloned from cultured Vitis vinifera L. cv Pinot Noir cells and used to probe the induction response of grapevine ...
PubMed:
9390436
PubMed Central:
PMC158566

40. Differential induction of lipoxygenase isoforms in wheat upon treatment with rust fungus elicitor, chitin oligosaccharides, chitosan, and methyl jasmonate

Author:
Bohland, C.; Balkenhohl, T.; Loers, G.; Feussner, I.; Grambow, H.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 679-685
ISSN:
0032-0889
Subject:
protein synthesis; dose response; chitosan; seedlings; jasmonic acid; linoleate 13S-lipoxygenase; Puccinia graminis; Triticum aestivum; leaves; plant pathogenic fungi; oligosaccharides; chitin; enzyme activity; isozymes; protein composition
Abstract:
... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...

41. Differential mRNA degradation of two beta-tubulin isoforms correlates with cytosolic Ca2+ changes in glucan-elicited soybean cells

Author:
Ebel, C.; Gomez, L.G.; Schmit, A.C.; Neuhaus-Url, G.; Boller, T.
Source:
Plant physiology 2001 v.126 no.1 pp. 87-96
ISSN:
0032-0889
Subject:
Glycine max; Phytophthora; plant pathogenic fungi; pathogenesis; glucans; cultured cells; cytoskeleton; cytosol; calcium; biosynthesis; quantitative analysis; tubulin; protein degradation; messenger RNA; transgenic plants; genetic variation; gene expression; chemical constituents of plants; transcription (genetics)
Abstract:
... Transgenic soybean (Glycine max) culture cells expressing apoaequorin, a Ca2+ indicator, were exposed to glucan fragments derived from Phytophthora sojae or to chitin oligomers. The effects of these elicitors on cytosolic Ca2+ concentrations and on mRNA levels of two beta-tubulin isoforms, tubB1 and tubB2, were investigated. The glucan elicitors, to which the cells are known to react with a biphas ...
PubMed:
11351073
PubMed Central:
PMC102284

42. Dissecting Defense-Related and Developmental Transcriptional Responses of Maize during Ustilago maydis Infection and Subsequent Tumor Formation

Author:
Basse, Christoph W.
Source:
Plant physiology 2005 v.138 no.3 pp. 1774-1784
ISSN:
0032-0889
Subject:
Zea mays; plant genetics; plant biochemistry; host-pathogen relationships; Glomerella graminicola; smut diseases; corn; transcription (genetics); microbial colonization; resistance mechanisms; plant pathogenic fungi; plant response; disease resistance; nucleotide sequences; hydroxamic acids; pathogenesis-related proteins; gene expression regulation; Ustilago zeae
Abstract:
... Infection of maize (Zea mays) plants with the smut fungus Ustilago maydis triggers the formation of tumors on aerial parts in which the fungal life cycle is completed. A differential display screen was performed to gain insight into transcriptional changes of the host response. Some of the genes strongly up-regulated in tumors showed a pronounced developmental expression pattern with decreasing tr ...
DOI:
10.1104/pp.105.061200
PubMed:
15980197
PubMed Central:
PMC1176445

43. A Domain-Centric Analysis of Oomycete Plant Pathogen Genomes Reveals Unique Protein Organization

Author:
Seidl, Michael F.; Van den Ackerveken, Guido; Govers, Francine; Snel, Berend
Source:
Plant physiology 2011 v.155 no.2 pp. 628-644
ISSN:
0032-0889
Subject:
proteins; host-pathogen relationships; nutrient uptake; Oomycetes; gene expression; pathogenicity; genes; plant pathogenic fungi; plant response; signal peptide
Abstract:
... Oomycetes comprise a diverse group of organisms that morphologically resemble fungi but belong to the stramenopile lineage within the supergroup of chromalveolates. Recent studies have shown that plant pathogenic oomycetes have expanded gene families that are possibly linked to their pathogenic lifestyle. We analyzed the protein domain organization of 67 eukaryotic species including four oomycete ...
DOI:
10.1104/pp.110.167841
PubMed:
21119047
PubMed Central:
PMC3032455

44. Ectopic Expression of Constitutively Activated RACB in Barley Enhances Susceptibility to Powdery Mildew and Abiotic Stress

Author:
Schultheiss, Holger; Hensel, Götz; Imani, Jafargholi; Broeders, Sylvia; Sonnewald, Uwe; Kogel, Karl-Heinz; Kumlehn, Jochen; Hůckelhoven, Ralph
Source:
Plant physiology 2005 v.139 no.1 pp. 353-362
ISSN:
0032-0889
Subject:
RNA interference; plant biochemistry; Blumeria graminis f. sp. hordei; G-proteins; plant pathogenic fungi; plant proteins; barley; stomatal conductance; Hordeum vulgare; powdery mildew; disease resistance; phenotypic variation; photosynthesis; plant development; gene overexpression; host plants; resistance mechanisms; water content; abiotic stress; genetic transformation; plant genetics; transgenes; host-pathogen relationships; transgenic plants; nucleotide sequences; plant morphology
Abstract:
... Small RAC/ROP-family G proteins regulate development and stress responses in plants. Transient overexpression and RNA interference experiments suggested that the barley (Hordeum vulgare) RAC/ROP protein RACB is involved in susceptibility to the powdery mildew fungus Blumeria graminis f. sp. hordei. We created transgenic barley plants expressing the constitutively activated RACB mutant racb-G15V un ...
DOI:
10.1104/pp.105.066613
PubMed:
16126850
PubMed Central:
PMC1203384

45. Elicitor activity of a fungal endopolygalacturonase in tobacco requires a functional catalytic site and cell wall localization

Author:
Boudart, G.; Charpentier, M.; Lafitte, C.; Martinez, Y.; Jauneau, A.; Gaulin, E.; Esquerre-Tugaye, M.T.; Dumas, B.
Source:
Plant physiology 2003 v.131 no.1 pp. 93-101
ISSN:
0032-0889
Subject:
beans; seedlings; polygalacturonase; necrosis; plant extracts; Phaseolus vulgaris; Colletotrichum lindemuthianum; plant pathogenic fungi; plant proteins; plant diseases and disorders; sugars; cell walls; Nicotiana tabacum; signal peptide; leaves; defense mechanisms; host plants; tobacco; transgenic plants; nucleotide sequences; plant morphology
Abstract:
... CLPG1, an endopolygalacturonase (endoPG) gene of Colletotrichum lindemuthianum, was transferred to tobacco (Nicotiana tabacum) leaves by using the Agrobacterium tumefaciens transient delivery system. The following four constructs were prepared: CLPG1, with or without its signal peptide (SP; PG1, PG1deltaSP); CLPG1 with the tobacco expansin1 SP instead of its own SP (Exp::PG1deltaSP); and a mutated ...
PubMed:
12529518
PubMed Central:
PMC166790

46. Elicitor-induced spruce stress lignin: structural similarity to early developmental lignins

Author:
Lange, B.M.; Lapierre, C.; Sandermann, H. Jr.
Source:
Plant physiology 1995 v.108 no.3 pp. 1277-1287
ISSN:
0032-0889
Subject:
Picea abies; cell suspension culture; wood; conifer needles; hypocotyls; lignin; biosynthesis; Dothideales; plant pathogenic fungi; developmental stages; quantitative analysis; carbohydrates; protein content; amino acids; chemical structure; cell wall components; stress response
Abstract:
... Suspension cultures of Picea abies (L.) Karst released polymeric material into the culture medium when treated with an elicitor preparation from the spruce needle pathogen Rhizosphaera kalkhoffi. The presence of lignin (about 35%, w/w) was demonstrated by phloroglucinol/HCl reactivity and quantitation with thioglycolic acid. Carbohydrate (about 14%, w/w) and protein (about 32%, w/w) were also dete ...

47. Enhancing Resistance to Sclerotinia minor in Peanut by Expressing a Barley Oxalate Oxidase Gene

Author:
Livingstone, D. Malcolm; Hampton, Jaime L.; Phipps, Patrick M.; Grabau, Elizabeth A.
Source:
Plant physiology 2005 v.137 no.4 pp. 1354-1362
ISSN:
0032-0889
Subject:
Hordeum vulgare; barley; grain crops; plant proteins; oxidoreductases; transgenes; gene expression; enzyme activity; Arachis hypogaea; peanuts; oil crops; transgenic plants; disease resistance; fungal diseases of plants; Sclerotinia minor; plant pathogenic fungi; oxalic acid; Southern blotting; Northern blotting; nucleotide sequences
Abstract:
... Sclerotinia minor Jagger is the causal agent of Sclerotinia blight, a highly destructive disease of peanut (Arachis hypogaea). Based on evidence that oxalic acid is involved in the pathogenicity of many Sclerotinia species, our objectives were to recover transgenic peanut plants expressing an oxalic acid-degrading oxalate oxidase and to evaluate them for increased resistance to S. minor. Transform ...
DOI:
10.1104/pp.104.057232
PubMed:
15778458
PubMed Central:
PMC1088326

48. Evaluation of the Antimicrobial Activities of Plant Oxylipins Supports Their Involvement in Defense against Pathogens

Author:
Prost, Isabelle; Dhondt, Sandrine; Rothe, Grit; Vicente, Jorge; Rodriguez, Maria Jose; Kift, Neil; Carbonne, Francis; Griffiths, Gareth; Esquerre-Tugaye, Marie-Therese; Rosahl, Sabine; Castresana, Carmen; Hamberg, Mats; Fournier, Joelle
Source:
Plant physiology 2005 v.139 no.4 pp. 1902-1913
ISSN:
0032-0889
Subject:
plant fats; unsaturated fatty acids; antibacterial properties; antifungal properties; plant pathogenic fungi; plant pathogenic bacteria
Abstract:
... Plant oxylipins are a large family of metabolites derived from polyunsaturated fatty acids. The characterization of mutants or transgenic plants affected in the biosynthesis or perception of oxylipins has recently emphasized the role of the so-called oxylipin pathway in plant defense against pests and pathogens. In this context, presumed functions of oxylipins include direct antimicrobial effect, ...
DOI:
10.1104/pp.105.066274
PubMed:
16299186
PubMed Central:
PMC1310568

49. Evidence for a Ustilago maydis steroid 5 alpha-reductase by functional expression in Arabidopsis det2-1 mutants

Author:
Basse, C.W.; Kerschbamer, C.; Brustmann, M.; Altmann, T.; Kahmann, R.
Source:
Plant physiology 2002 v.129 no.2 pp. 717-732
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transgenic plants; flowering; seed development; mutants; Zea mays; corn; Ustilago zeae; plant pathogenic fungi; single cell protein; oxidoreductases; recombinant proteins; gene expression; endoplasmic reticulum; plant morphology; light; amino acid sequences; nucleotide sequences; host plants; plant diseases and disorders; genetic complementation; green fluorescent protein
Abstract:
... We have identified a gene (udh1) in the basidiomycete Ustilago maydis that is induced during the parasitic interaction with its host plant maize (Zea mays). udh1 encodes a protein with high similarity to mammalian and plant 5 alpha-steroid reductases. Udh1 differs from those of known 5 alpha-steroid reductases by six additional domains, partially predicted to be membrane-spanning. A fusion protein ...
PubMed:
12068114
PubMed Central:
PMC161696

50. Evidence of phytoalexins in cucumber leaves infected with powdery mildew following treatment with leaf extracts of Reynoutria sachalinensis

Author:
Daayf, F.; Schmitt, A.; Belanger, R.R.
Source:
Plant physiology 1997 v.113 no.3 pp. 719-727
ISSN:
0032-0889
Subject:
Podosphaera xanthii; biosynthesis; quantitative analysis; Cucumis sativus; antifungal properties; defense mechanisms; phenolic compounds; pathogenicity; plant pathogenic fungi; disease resistance; hydrolysis; Reynoutria sachalinensis; enzyme activity; leaves; phytoalexins
Abstract:
... Phenolic compounds extracted from cucumber (Cucumis sativus L.) leaves were separated and analyzed for their differential presence and fungitoxicity in relation to a prophylactic treatment withMilsana (Compo, Munster, Germany) against powdery mildew (Sphaerotheca fuliginea). Based on our extraction and purification procedures, at least eight separate phenolic compounds with antifungal activity wer ...