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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Subject term Brassica napus Remove constraint Subject term: Brassica napus Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Embryo-specific gene expression in microspore-derived embryos of Brassica napus. An interaction between abscisic acid and jasmonic acid

Author:
Hays, D.B.; Wilen, R.W.; Sheng, C.X.; Moloney, M.M.; Pharis, R.P.
Source:
Plant physiology 1999 v.119 no.3 pp. 1065-1072
ISSN:
0032-0889
Subject:
Brassica napus; gene expression; embryo (plant); spores; abscisic acid; jasmonic acid; plant proteins; messenger RNA; biosynthesis; application rate
Abstract:
... The induction of napin and oleosin gene expression in Brassica napus microspore-derived embryos (MDEs) was studied to assess the possible interaction between abscisic acid (ABA) and jasmonic acid (JA). Napin and oleosin transcripts were detected sooner following treatment with ABA than JA. Treatment of MDEs with ABA plus JA gave an additive accumulation of both napin and oleosin mRNA, the absolute ...

2. Osmotic water permeability of isolated protoplasts. Modifications during development

Author:
Ramahaleo, T.; Morillon, R.; Alexandre, J.; Lassalles, J.P.
Source:
Plant physiology 1999 v.119 no.3 pp. 885-896
ISSN:
0032-0889
Subject:
Triticum aestivum; Zea mays; Brassica napus; protoplasts; plant development; osmosis; roots; active transport; membrane permeability; plasma membrane
Abstract:
... A transference chamber was developed to measure the osmotic water permeability coefficient (P(os)) in protoplasts 40 to 120 micrometer in diameter. The protoplast was held by a micropipette and submitted to a steep osmotic gradient created in the transference chamber. P(os) was served from the changes in protoplast dimensions, as measured using a light microscope. Permeabilities were in the range ...

3. Phloem transport of D,L-glufosinate and acetyl-L-glufosinate in glufosinate-resistant and -susceptible Brassica napus

Author:
Beriault, J.N.; Horsman, G.P.; Devine, M.D.
Source:
Plant physiology 1999 v.121 no.2 pp. 619-627
ISSN:
0032-0889
Subject:
anthers; glutamine; transgenic plants; isomers; flowers; translocation (plant physiology); phloem; glufosinate; genotype; Brassica napus; herbicide resistance; roots; leaves; acyltransferases
Abstract:
... Phloem transport of D,L-[(14)C]glufosinate, D-[(14)C]glufosinate, and acetyl-L-[(14)C]glufosinate, was examined in the susceptible Brassica napus cv Excel and a glufosinate-resistant genotype (HCN27) derived by transformation of cv Excel with the phosphinothricin-N-acetyltransferase (pat) gene. Considerably more (14)C was exported from an expanded leaf in HCN27 than in cv Excel following applicati ...

4. (+)-Abscisic acid metabolism, 3-ketoacyl-coenzyme A synthase gene expression, and very-long-chain monounsaturated fatty acid biosynthesis in Brassica napus embryos

Author:
Qi, Q.; Rose, P.A.; Abrams, G.D.; Taylor, D.C.; Abrams, S.R.; Cutler, A.J.
Source:
Plant physiology 1998 v.117 no.3 pp. 979-987
ISSN:
0032-0889
Subject:
biosynthesis; enzymes; embryo (plant); long chain fatty acids; coenzyme A; transcription (genetics); gene expression; monounsaturated fatty acids; genes; Brassica napus; abscisic acid; enzyme activity; chemical structure
Abstract:
... Microspore-derived embryos of Brassica napus cv Reston were used to examine the effects of exogenous (+)-abscisic acid (ABA)and related compounds on the accumulation of very-long-chain monounsaturated fatty acids (VLCMFAs), VLCMFA elongase complex activity, and induction of the 3-ketoacyl-coenzyme A synthase (KCS) gene encoding the condensing enzyme of the VLCMFA elongation system. Of the concentr ...

5. Characterization of stomatal closure caused by ultraviolet-B radiation

Author:
Nogues, S.; Allen, D.J.; Morison, J.I.L.; Baker, N.R.
Source:
Plant physiology 1999 v.121 no.2 pp. 489-496
ISSN:
0032-0889
Subject:
stomatal movement; interspecific variation; stomatal conductance; Commelina communis; surfaces; photosynthesis; Brassica napus; Pisum sativum; mesophyll; ultraviolet radiation; stomata
Abstract:
... The effects of ultraviolet-B (UV-B) radiation on stomatal conductance (g(s)) in pea (Pisum sativum L.), commelina (Commelina communis L.), and oilseed rape (Brassica napus L.) plants were investigated. Plants were grown in a greenhouse either with three different high ratios of UV-B to photosynthetically active radiation or with no UV-B radiation. Pea plants grown in the highest UV-B radiation (0. ...

6. Decreased cell wall digestibility in canola transformed with chimeric tyrosine decarboxylase genes from opium poppy

Author:
Facchini, P.J.; Yu, M.; Penzes-Yost, C.
Source:
Plant physiology 1999 v.120 no.3 pp. 653-663
ISSN:
0032-0889
Subject:
Brassica napus; cell walls; in vitro digestibility; genetic transformation; transgenic plants; carboxy-lyases; genes; defense mechanisms; isozymes; enzyme activity; messenger RNA; protein synthesis; tyramine; aromatic compounds; cell wall components; transcription (genetics); promoter regions
Abstract:
... Tyrosine decarboxylase (TYDC) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. Although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found as integral cell wall components. Canola (Brassica napus) was independently transf ...

7. Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels

Author:
Knutzon, D.S.; Hayes, T.R.; Wyrick, A.; Xiong, H.; Davies, H.M.; Voelker, T.A.
Source:
Plant physiology 1999 v.120 no.3 pp. 739-746
ISSN:
0032-0889
Subject:
Brassica napus; seed development; Umbellularia californica; dodecanoic acid; Cocos nucifera; endosperm; triacylglycerols; rapeseed oil; chemical composition; transgenic plants; acyltransferases; gene expression; genetic engineering
Abstract:
... Expression of a California bay laurel (Umbellularia californica) 12:0-acyl-carrier protein thioesterase, bay thioesterase (BTE), in developing seeds of oilseed rape (Brassica napus) led to the production of oils containing up to 50% laurate. In these BTE oils, laurate is found almost exclusively at the sn-1 and sn-3 positions of the triacylglycerols (T.A. Voelker, T.R. Hayes, A.C. Cranmer, H.M. Da ...

8. Regulation of apoplastic NH4+ concentration in leaves of oilseed rape

Author:
Nielsen, K.H.; Schjoerring, J.K.
Source:
Plant physiology 1998 v.118 no.4 pp. 1361-1368
ISSN:
0032-0889
Subject:
Brassica napus; leaves; ion transport; temperature; pH; chemical reactions; mathematical models; equations; plasma membrane; chemical constituents of plants; ammonium compounds
Abstract:
... Regulation of apoplastic NH4+ concentration in leaves of oilseed rape (Brassica napus L.) was studied using a vacuum-infiltration technique that allowed controlled manipulations of the apoplastic solution. In leaves infiltrated with NH4+ -free solution, the apoplastic NH4+ concentration returned in less than 1.5 minto the preinfiltration level of 0.8 mM. Infiltrated 15NH4+ was rapidly diluted by 1 ...

9. Uridine 5'-diphosphate-glucose dehydrogenase from soybean nodules

Author:
Stewart, D.C.; Copeland, L.
Source:
Plant physiology 1998 v.116 no.1 pp. 349-355
ISSN:
0032-0889
Subject:
Glycine max; Hordeum vulgare; symbiosis; glucose; developmental stages; root nodules; physicochemical properties; oxidoreductases; Bradyrhizobium japonicum; Triticum aestivum; Brassica napus; kinetics; enzyme activity; protein composition
Abstract:
... A highly purified preparation of uridine 5'-diphosphate (UDP)-glucose (Glc) dehydrogenase (DH; EC 1.1.1.22) has been characterized from soybean (Glycine max L.) nodules. The enzyme had native and subunit molecular masses of approximately 272 and 50 kD, respectively. UDP-Glc DH displayed typical hyperbolic substrate kinetics and had Km values for UDP-Glc and NAD+ of 0.05 and 0.12 mM, respectively. ...

10. Distribution of sulfur within oilseed rape leaves in response to sulfur deficiency during vegetative growth

Author:
Blake-Kalff, M.M.A.; Harrison, K.R.; Hawkesford, M.J.; Zhao, F.J.; McGrath, S.P.
Source:
Plant physiology 1998 v.118 no.4 pp. 1337-1344
ISSN:
0032-0889
Subject:
Brassica napus; leaves; sulfur; sulfates; glucosinolates; glutathione; quantitative analysis; nutrient deficiencies; chemical constituents of plants; stress response
Abstract:
... The distribution of S to sulfate, glucosinolates, glutathione, and the insoluble fraction within oilseed rape (Brassica napus L.) leaves of different ages was investigated during vegetative growth. The concentrations of glutathione and glucosinolates increased from the oldest to the youngest leaves, whereas the opposite was observed for SO4(2-). The concentration of insoluble S was similar among a ...

11. Fatty acid elongation is independent of acyl-coenzyme A synthetase activities in leek and Brassica napus

Author:
Hlousek-Radojcic, A.; Evenson, K.J.; Jaworski, J.G.; Post-Beittenmiller, D.
Source:
Plant physiology 1998 v.116 no.1 pp. 251-258
ISSN:
0032-0889
Subject:
Allium porrum; biosynthesis; phosphatidylcholines; stearic acid; microsomes; Brassica napus; malonic acid; enzyme activity; long-chain-fatty-acid-CoA ligase
Abstract:
... In both animal and plant acyl elongation systems, it has been proposed that fatty acids are first activated to acyl-coenzyme A (CoA) before their elongation, and that the ATP dependence of fatty acid elongation is evidence of acyl-CoA synthetase involvement. However, because CoA is not supplied in standard fatty acid elongation assays, it is not clear if CoA-dependent acyl-CoA synthetase activity ...

12. Glutathione-mediated regulation of ATP-sulfurylase activity, SO4(2-) uptake, and oxidative stress response in intact canola roots

Author:
Lappartient, A.G.; Touraine, B.
Source:
Plant physiology 1997 v.114 no.1 pp. 177-183
ISSN:
0032-0889
Subject:
sulfur dioxide; sulfur; metabolism; glutathione; quantitative analysis; glutathione-disulfide reductase; hydrogen peroxide; nutrient deficiencies; stress response; ion transport; adenosine triphosphate; peroxidases; phloem; Brassica napus; oxidation; phosphotransferases (kinases); enzyme activity; roots; ascorbic acid
Abstract:
... The dual role of glutathione as a transducer of S status (A.G. Lappartient and B. Touraine [1996] Plant Physiol 111:147-157) and as an antioxidant was examined by comparing the effects of S deprivation, glutathione feeding, and H2O2 (oxidative stress) on SO42- uptake and ATP sulfurylase activity in roots of intact canola (Brassica napus L.). ATP sulfurylase activity increased and SO42- uptake rate ...

13. Metabolic bypass of the tricarboxylic acid cycle during lipid mobilization in germinating oilseeds. Regulation of NAD+-dependent isocitrate dehydrogenase versus fumarase

Author:
Falk, K.L.; Behal, R.H.; Xiang, C.; Oliver, D.J.
Source:
Plant physiology 1998 v.117 no.2 pp. 473-481
ISSN:
0032-0889
Subject:
Brassica napus; seed germination; imbibition; developmental stages; lipid metabolism; sucrose; biosynthesis; triacylglycerols; tricarboxylic acid cycle; isocitrate dehydrogenase; enzyme activity; measurement; light; messenger RNA; transcription (genetics)
Abstract:
... Biosynthesis of sucrose from triacylglycerol requires the bypass of the CO2-evolving reactions of the tricarboxylic acid (TCA)cycle. The regulation of the TCA cycle bypass during lipid mobilization was examined. Lipid mobilization in Brassica napus was initiated shortly after imbibition of the seed and proceeded until 2 d postimbibition, as measured by in vivo [1-(14)C]acetate feeding to whole see ...

14. Partial purification and characterization of red chlorophyll catabolite reductase, a stroma protein involved in chlorophlly breakdown

Author:
Rodoni, S.; Vicentini, F.; Schellenberg, M.; Matile, P.; Hortensteiner, S.
Source:
Plant physiology 1997 v.115 no.2 pp. 677-682
ISSN:
0032-0889
Subject:
chloroplasts; metabolism; Hordeum vulgare; Capsicum annuum; fluorescence; developmental stages; oxygen requirement; chemical reactions; cotyledons; metabolites; biochemical pathways; cell membranes; Brassica napus; kinetics; chlorophyll; roots; chemical structure; leaves
Abstract:
... Red chlorophyll (Chl) catabolite (RCC) reductase, which catalyzes the reaction of an intermediary Chl catabolite (RCC) in the two-step cleavage reaction of pheophorbide (Pheide) a into primary fluorescent catabolites (pFCCs) during Chl breakdown, was characterized and partially purified. RCC reductase activity was present at all stages of barley leaf development and even in roots. The highest spec ...

15. Carbohydrate content and enzyme metabolism in developing canola siliques

Author:
King, S.P.; Lunn, J.E.; Furbank, R.T.
Source:
Plant physiology 1997 v.114 no.1 pp. 153-160
ISSN:
0032-0889
Subject:
source-sink relationships; carbohydrate metabolism; cultivars; seeds; quantitative analysis; chemical constituents of plants; cell walls; sucrose; developmental stages; photosynthates; starch; beta-fructofuranosidase; Brassica napus; genetic variation; enzyme activity; sucrose synthase; pods
Abstract:
... Little biochemical information is available on carbohydrate metabolism in developing canola (Brassica napus L.) silique (pod) wall and seed tissues. This research examines the carbohydrate contents and sucrose (Suc) metabolic enzyme activities in different aged silique wall and seed tissues during oil filling. The silique wall partitioned photosynthate into Suc over starch and predominantly accumu ...

16. Chlorophyll breakdown in senescent chloroplasts

Author:
Rodoni, S.; Muhlecker, W.; Anderl, M.; Krautler, B.; Moser, D.; Thomas, H.; Matile, P.; Hortensteiner, S.
Source:
Plant physiology 1997 v.115 no.2 pp. 669-676
ISSN:
0032-0889
Subject:
chloroplasts; metabolism; Hordeum vulgare; Capsicum annuum; fluorescence; chemical reactions; cotyledons; biochemical pathways; cell membranes; Brassica napus; enzyme activity; chlorophyll; oxygenases; chemical structure; metabolites
Abstract:
... The cleavage of pheophorbide (Pheide) a into primary fluorescent chlorophyll (Chl) catabolites (pFCCs) in senescent chloroplasts was investigated. Chloroplast preparations isolated fromsenescent canola (Brassica napus) cotyledons exhibited light-dependent production of pFCC when assay mixtures were supplemented with ferredoxin (Fd). pFCC production in detergent-solubilized membranes was dependent ...

17. Demand-driven control of root ATP sulfurylase activity and SO4(2-) uptake in intact canola: the role of phloem-translocated glutathione

Author:
Lappartient, A.G.; Touraine, B.
Source:
Plant physiology 1996 v.111 no.1 pp. 147-157
ISSN:
0032-0889
Subject:
Brassica napus; roots; shoots; sulfur dioxide; nutrient uptake; adenosine triphosphate; transferases; enzyme activity; phloem; glutathione; sulfur; nutrient deficiencies; nitrates; translocation (plant physiology); chemical constituents of plants; phosphates; binding sites
Abstract:
... The activity of ATP sulfurylase extracted from roots of intact canola (Brassica napus L. cv Drakkar) increased after withdrawal of the S source from the nutrient solution and declined after refeeding SO4(2-) to S-starved plants. The rate of SO4(2-) uptake by the roots was similarly influenced. Identical responses were obtained in SO4(2-)-fed roots when one-half of the root system was starved for S ...

18. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

19. Insect control and dosage effects in transgenic canola containing a synthetic Bacillus thuringiensis cryIAc gene

Author:
Stewart, C.N. Jr.; Adang, M.J.; All, J.N.; Raymer, P.L.; Ramachandran, S.; Parrott, W.A.
Source:
Plant physiology 1996 v.112 no.1 pp. 115-120
ISSN:
0032-0889
Subject:
Helicoverpa zea; genetic transformation; antibiosis; insect pests; Trichoplusia ni; structural genes; transgenic plants; Agrobacterium radiobacter; Spodoptera exigua; gene expression; Plutella xylostella; Bacillus thuringiensis; crystal proteins; Brassica napus; insect control; gene dosage
Abstract:
... Zygotic hypocotyls of canola (Brassica napus L.) cv Oscar, cv Westar, and the breeding line UGA188-20B were transformed with a truncated synthetic Bacillus thuringiensis insecticidal crystal protein gene (Bt cryIAc) under the control of the cauliflower mosaic virus 35S promoter using Agrobacterium tumefaciens-mediated transformation. Fifty-seven independently transformed lines were produced, conta ...

20. Molecular characerization of two Brassica napus pollen-expressed genes encoding putative arabinogalactan proteins

Author:
Gerster, J.; Allard, S.; Robert, L.S.
Source:
Plant physiology 1996 v.110 no.4 pp. 1231-1237
ISSN:
0032-0889
Subject:
developmental stages; genes; Brassica napus; pollen; plant proteins; threonine; arabinose; alanine; proline; clones; gene expression; cysteine; histidine; mitosis; messenger RNA; galactans; complementary DNA; genetic code; tyrosine; pollen tubes; amino acid sequences; transcription (genetics); tryptophan; nucleotide sequences; genetic techniques and protocols; chemical constituents of plants
Abstract:
... Two highly homologous cDNA clones, Sta 39-3 and Sta 39-4, corresponding to mRNAs highly expressed in Brassica napus cv Westar stamens were isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 39-3 and Sta 39-4 transcripts accumulate in pollen following the first pollen mitosis and are abundant at pollen maturity, thus identifying them as "l ...

21. Ammonia flux between oilseed rape plants and the atmosphere in response to changes in leaf temperature, light intensity, and air humidity. Interactions with leaf conductance and apoplastic NH4+ and H+ concentrations

Author:
Husted, S.; Schjoerring, J.K.
Source:
Plant physiology 1996 v.112 no.1 pp. 67-74
ISSN:
0032-0889
Subject:
Brassica napus; ammonia; gas exchange; leaves; temperature; light intensity; relative humidity; leaf conductance
Abstract:
... NH3 exchange between oilseed rape (Brassica napus) plants and the atmosphere was examined at realistic ambient NH3 levels under controlled environmental conditions. Different leaf conductances to NH3 diffusion were obtained by changing leaf temperature (10 to 40 degrees C), light intensity (0 to 600 micromoles m-2 s-1), and air humidity (20 to 80%), respectively. NH3 adsorption to the cuticle with ...

22. Cold hardening of spring and winter wheat and rape results in differential effects on growth, carbon metabolism, and carbohydrate content

Author:
Hurry, V.M.; Strand, A.; Tobiaeson, M.; Gardestrom, P.; Oquist, G.
Source:
Plant physiology 1995 v.109 no.2 pp. 697-706
ISSN:
0032-0889
Subject:
freezing; cultivars; seedling growth; interspecific variation; biosynthesis; plant nutrition; seedlings; fluorescence; carbohydrates; net assimilation rate; gas exchange; biochemical pathways; carbon; Triticum aestivum; Brassica napus; enzyme activity; chlorophyll; cold tolerance; metabolites
Abstract:
... The effect of long-term (months) exposure to low temperature (5 degrees C) on growth,photosynthesis, and carbon metabolism was studied in spring and winter cultivars of wheat (Triticum aestivum) and rape (Brassica napus). Cold-grown winter rape and winter wheat maintained higher net assimilation rates and higher in situ CO2 exchange rates than the respective cold-grown spring cultivars. In particu ...

23. Glucosinolate biosynthesis. Further characterization of the aldoxime-forming microsomal monooxygenases in oilseed rape leaves

Author:
Bennett, R.N.; Hick, A.J.; Dawson, G.W.; Wallsgrove, R.M.
Source:
Plant physiology 1995 v.109 no.1 pp. 299-305
ISSN:
0032-0889
Subject:
oximes; sulfur; biosynthesis; chemical reactions; NADP (coenzyme); microsomes; methionine; Brassica napus; oxygen; enzyme activity; molecular conformation; oxygenases; leaves; glucosinolates
Abstract:
... The initial steps in glucosinolate biosynthesis are thought to proceed from amino acids, via N-hydroxy amino acids, to aldoximes. We showed previously that microsomes from green leaves of oilseed rape (Brassica napus cv Bienvenu) contain two distinct monooxygenases that catalyze the conversion of homophenylalanine and dihomomethionine to their respective aldoximes. Further characterization of thes ...

24. In vivo photomodification of ribulose-1,5-bisphosphate carboxylase/oxygenase holoenzyme by ultraviolet-B radiation. Formation of a 66-kilodalton variant of the large subunit

Author:
Wilson, M.I.; Ghosh, S.; Gerhardt, K.E.; Holland, N.; Sudhakar Babu, T.; Edelman, M.; Dumbroff, E.B.; Greenberg, B.M.
Source:
Plant physiology 1995 v.109 no.1 pp. 221-229
ISSN:
0032-0889
Subject:
Brassica napus; ultraviolet radiation; light; ribulose-bisphosphate carboxylase; plant proteins; molecular weight; mutants; oxidation; biosynthesis
Abstract:
... Increased levels of solar ultraviolet (290-320 nm) (UV-B) radiation could have profound effects on plant proteins because the aromatic amino acids in proteins absorb strongly in this spectral region. We have investigated the effects of UV-B radiation on plant proteins and have observed a novel 66-kD protein. This product was formed in vivo when Brassica napus L. plants grown for 21 d in 65 micromo ...

25. Apoplastic pH and ammonium concentration in leaves of Brassica napus L

Author:
Husted, S.; Schjoerring, J.K.
Source:
Plant physiology 1995 v.109 no.4 pp. 1453-1460
ISSN:
0032-0889
Subject:
Brassica napus; methodology; solutions; water content; pH; glutamate-ammonia ligase; enzyme activity; ammonia; gas exchange; enzyme inhibitors; leaves; seedlings; ammonium compounds; solutes
Abstract:
... A vacuum infiltration technique was developed that enabled the extraction of apoplastic solution with very little cytoplasmic contamination as evident from a malate dehydrogenase activity of less than 1% in the apoplastic solution relative to that in bulk leaf extracts. The volume of apoplastic water, a prerequisite for determination of the concentration of apoplastic solutes, was determined by va ...

26. Cold-induced accumulation of hsp90 transcripts in Brassica napus

Author:
Krishna, P.; Sacco, M.; Cherutti, J.F.; Hill, S.
Source:
Plant physiology 1995 v.107 no.3 pp. 915-923
ISSN:
0032-0889
Subject:
Brassica napus; Spinacia oleracea; seedlings; cell suspension culture; genetic code; genes; heat shock proteins; gene expression; cold; protein content; messenger RNA; cold tolerance; acclimation; transcription (genetics); stress response
Abstract:
... Characterization of the expression of hsp90 genes of Brassica napus by northern blot analysis and immunoblotting showed that the hsp90 mRNA and protein are present in all B. napus tissues examined, albeit at different levels. High levels of hsp90 mRNA and protein were found in young and rapidly dividing tissues such as shoot apices and flower buds, suggesting that hsp90 may have an important role ...

27. Plant microsomal phospholipid acyl hydrolases have selectivities for uncommon fatty acids

Author:
Stahl, U.; Banas, A.; Stymne, S.
Source:
Plant physiology 1995 v.107 no.3 pp. 953-962
ISSN:
0032-0889
Subject:
Cuphea; Ulmus glabra; Euphorbia; Ricinus communis; Brassica napus; seed development; seeds; microsomes; phospholipids; metabolism; hydrolases; enzyme activity; fatty acids; short chain triacylglycerols; medium chain triacylglycerols; long chain triacylglycerols
Abstract:
... Developing endosperms and embryos accumulating triacylglycerols rich in caproyl (decanoyl) groups (i.e. developing embryos of Cuphea procumbens and Ulmus glabra) had microsomal acyl hydrolases with high selectivities toward phosphatidylcholine with this acyl group. Similarly, membranes from Euphorbia lagascae and Ricinus communis endosperms, which accumulate triacylglycerols with vernoleate (12-ep ...

28. Zinc requirement for stomatal opening in cauliflower

Author:
Sharma, P.N.; Tripathi, A.; Bisht, S.S.
Source:
Plant physiology 1995 v.107 no.3 pp. 751-756
ISSN:
0032-0889
Subject:
Brassica napus; Brassica oleracea var. botrytis; stomatal movement; zinc; dose response; nutrient deficiencies; leaves; waxes; biosynthesis; proline; carbonate dehydratase; enzyme activity; leaf water potential; guard cells; potassium; chemical constituents of plants; thickness
Abstract:
... Zn deficiency induced increases in epicuticular wax deposits, lamina thickness, degree of succulence, water saturation deficit, diffusive resistance, and proline accumulation and decreases in carbonic anhydrase activity, water potential, stomatal aperture, and transpiration in the leaves of cauliflower (Brassica oleracea L. var botrytis cv Pusa) plants. Restoration of Zn supply to the deficient pl ...

29. Light regulation of leaf mitochondrial pyruvate dehydrogenase complex: Role of photorespiratory carbon metabolism

Author:
Gemel, J.; Randall, D.D.
Source:
Plant physiology 1992 v.100 no.2 pp. 908-914
ISSN:
0032-0889
Subject:
pyruvate decarboxylase; photorespiration; light intensity; Cucurbita pepo; mitochondria; Brassica napus; oxidation; glycine (amino acid); Pisum sativum; enzyme activity; Brassica rapa subsp. oleifera
Abstract:
... Light-dependent inactivation of mitochondrial pyruvate dehydrogenase complex (MTPDC) in pea (Pisum sativum L.) leaves was further characterized, and this phenomenon was extended to several monocot and dicot species. The light-dependent inactivation of mtPDC in vivo was rapidly reversed in the dark, even after prolonged illumination. The mtPDC can be efficiently cycled through the inactivated-react ...
DOI:
10.1104/pp.100.2.908
PubMed:
16653075

30. Pleiotropy in triazine-resistant Brassica napus. Ontogenetic and diurnal influences on photosynthesis

Author:
Dekker, J.H.; Burmester, R.G.
Source:
Plant physiology 1992 v.100 no.4 pp. 2052-2058
ISSN:
0032-0889
Subject:
Brassica napus; pleiotropy; structural genes; herbicide resistance; triazine herbicides; photosynthesis; diurnal variation; biotypes; mutation; chloroplast DNA; genomics
Abstract:
... Studies were conducted that supported the hypothesis that the mutation to the psbA plastid gene that confers S-triazine resistance (R) in Brassica napus also results in an altered diurnal pattern of photosynthetic carbon assimilation (A) relative to that of the susceptible (S) wild type, and that these patterns change over the ontogeny of a plant. Photosynthetic photon flux density, under closely ...
DOI:
10.1104/pp.100.4.2052
PubMed:
16653239