Journal: Veterinary immunology and immunopathology / Subject: Western blotting / Text Availability: Citation in PubAg

1. Characterisation of antisera to recombinant IgA of the common brushtail possum (Trichosurus vulpecula)

Author:: Rawson, Renee R.; Belov, Katherine; Gidley-Baird, A. Angus; Cooper, Desmond W.
Source:: Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 89-95
ISSN:: 0165-2427
Subject:: Macropus eugenii; Macropus giganteus; Phascolarctos cinereus; Trichosurus vulpecula; Western blotting; antiserum; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin A; immunoglobulin G; mucosal immunity; possums; recombinant proteins
Abstract:: ... One of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. This paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of IgA (Cα) of the common brushtail possum (Trichosurus vulpecula). The availability of a marsupial specific anti-IgA provide ...
DOI:: 10.1016/S0165-2427(02)00126-5
: https://doi.org/10.1016/S0165-2427(02)00126-5

2. Cloning, expression, purification, and biological activity of five feline type I interferons

Author:: Wonderling, Ramani; Powell, Tim; Baldwin, Susan; Morales,Tony; Snyder, Scott; Keiser, Kathy; Hunter, Shirley; Best, Elaine; McDermott, Martin J.; Milhausen, Michael
Source:: Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 13-27
ISSN:: 0165-2427
Subject:: sequence analysis; interferon-alpha; antiviral properties; Komagataella pastoris; complementary DNA; cats; cell culture; glycosylation; humans; recombinant proteins; yeasts; Western blotting; amino acid sequences; amino acids; clones; genes; viruses; immune response
Abstract:: ... Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-α exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-α (feIFN) subtypes that share from 9 ...
DOI:: 10.1016/S0165-2427(02)00188-5
: https://doi.org/10.1016/S0165-2427(02)00188-5

3. Development and characterization of monoclonal antibodies to chicken interleukin-15

Author:: Min, Wongi; Lillehoj, Hyun S.; Li, Guangxing; Sohn, Eun J.; Miyamoto, Tadashi
Source:: Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 49-56
ISSN:: 0165-2427
Subject:: T-lymphocytes; Western blotting; cell growth; chickens; enzyme-linked immunosorbent assay; genes; interleukin-15; interleukin-2; monoclonal antibodies; polypeptides
Abstract:: ... The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-1 ...
DOI:: 10.1016/S0165-2427(02)00123-X
: https://doi.org/10.1016/S0165-2427(02)00123-X

4. Identification of monoclonal antibodies that cross-react with cytokines from different animal species

Author:: Pedersen, L.G.; Castelruiz, Y.; Jacobsen, S.; Aasted, B.
Source:: Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 111-122
ISSN:: 0165-2427
Subject:: Western blotting; cattle; dogs; flow cytometry; goats; granulocyte-macrophage colony-stimulating factor; horses; humans; interferon-gamma; interleukin-2; interleukin-4; interleukin-6; interleukin-8; mink; monoclonal antibodies; mononuclear leukocytes; rabbits; sheep; swine; tumor necrosis factor-alpha
Abstract:: ... Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-γ and TNF-α cross-reacted with cytokines from a majority of the species investiga ...
DOI:: 10.1016/S0165-2427(02)00139-3
: https://doi.org/10.1016/S0165-2427(02)00139-3

5. Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86)

Author:: Patarroyo, J.H.; Portela, R.W.; Castro, R.O. De; Pimentel, J. Couto; Guzman, F.; Patarroyo, M.E.; Vargas, M.I.; Prates, A.A.; Mendes, M.A. Dias
Source:: Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 163-172
ISSN:: 0165-2427
Subject:: Rhipicephalus microplus; Western blotting; antibodies; cattle; digestive system; females; glycoproteins; immune response; immunization; immunoglobulin G; oviposition; saponins; synthetic peptides; ticks
Abstract:: ... Three synthetic peptides (SBm4912, SBm7462 and SBm19733), derived from the Bm86 glycoprotein from Boophilus microplus gut, were constructed and used to immunize cattle from a tick-free area. The immunized animals received three subcutaneous doses of the peptides, with saponin as adjuvant, at 30-day intervals. The immune response was evaluated by IgG elicited against the peptides by the detection o ...
DOI:: 10.1016/S0165-2427(02)00154-X
: https://doi.org/10.1016/S0165-2427(02)00154-X

6. Mannheimia haemolytica serotype 1 and Pasteurella trehalosi serotype 10 culture supernatants contain fibrinogen-binding proteins

Author:: McNeil, Heather J.; Shewen, Patricia E.; Lo, Reggie Y.C.; Conlon, Jennifer A.; Miller, Michael W.
Source:: Veterinary immunology and immunopathology 2002 v.90 no.1-2 pp. 107-110
ISSN:: 0165-2427
Subject:: Mannheimia haemolytica serotype 1; Pasteurella trehalosi; Western blotting; disease control; fibrinogen; pneumonia; serotypes; virulence
Abstract:: ... Fibrinogen-binding proteins were found in the culture supernatants of Mannheimia haemolytica serotype 1 (ATCC 43270) and Pasteurella trehalosi serotype 10 (ECO-100). Sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. Fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteris ...
DOI:: 10.1016/S0165-2427(02)00222-2
: https://doi.org/10.1016/S0165-2427(02)00222-2

7. Role of bovine chemokines produced by dendritic cells in respiratory syncytial virus-induced T cell proliferation

Author:: Werling, D.; Koss, M.; Howard, C.J.; Taylor, G.; Langhans, W.; Hope, J.C.
Source:: Veterinary immunology and immunopathology 2002 v.87 no.3-4 pp. 225-233
ISSN:: 0165-2427
Subject:: chemokine CCL5; coculture; Bovine orthopneumovirus; cattle; dendritic cells; inflammation; reverse transcriptase polymerase chain reaction; Western blotting; viruses; CD4-positive T-lymphocytes; cell proliferation
Abstract:: ... Respiratory syncytial virus (RSV) has been reported to induce the production of chemokines in the airway epithelia. Dendritic cells (DC) are the most potent antigen-presenting cells. They are located throughout the body and release chemokines in response to inflammation and infection. We have investigated the chemokine profile of bovine DC in response to exposure to bovine RSV (BRSV). Transcripts ...
DOI:: 10.1016/S0165-2427(02)00086-7
: https://doi.org/10.1016/S0165-2427(02)00086-7

8. Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Author:: Paule, B.J.A.; Azevedo,V.; Regis, L.F.; Carminati, R.; Bahia, C.R.; Vale, V.L.C.; Moura-Costa, L.F.; Freire, S.M.; Nascimento, I.; Schaer, R.; Goes, A.M.; Meyer, R.
Source:: Veterinary immunology and immunopathology 2003 v.96 no.3-4 pp. 129-139
ISSN:: 0165-2427
Subject:: Corynebacterium pseudotuberculosis; Western blotting; antibodies; antigens; caseous lymphadenitis; cell-mediated immunity; enzyme-linked immunosorbent assay; goats; humoral immunity; immunoglobulin G; interferon-gamma; necropsy; sheep; virulence; Brazil
Abstract:: ... Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goat breeders in northern Brazil. The present work was conducted to analyse aspects of humoral and cellular immune responses after experimental infection. Eight goats were infected intradermally with a single do ...
DOI:: 10.1016/S0165-2427(03)00146-6
: https://doi.org/10.1016/S0165-2427(03)00146-6

9. Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE

Author:: Wagner, Bettina; Radbruch, Andreas; Rohwer, Jens; Leibold, Wolfgang
Source:: Veterinary immunology and immunopathology 2003 v.92 no.1-2 pp. 45-60
ISSN:: 0165-2427
Subject:: Icelandic (horse breed); Western blotting; adults; basophils; blood serum; eczema; enzyme-linked immunosorbent assay; eosinophils; epitopes; fluorescent antibody technique; foals; hypersensitivity; immunoglobulin A; immunoglobulin E; immunoglobulin G; immunoglobulin M; immunoglobulin heavy chains; lymphocytes; mice; monoclonal antibodies; monocytes; sorting
Abstract:: ... In this study we describe the generation of monoclonal antibodies (mAbs), which recognize different epitopes of the equine IgE constant heavy chain. Equi-murine recombinant IgE (rIgE), composed of the murine V(H)186.2 heavy chain variable region, linked to the equine IgE constant heavy chain and expressed together with the murine λ1 chain in J558L cells was used to immunize BALB/C mice. A total of ...
DOI:: 10.1016/S0165-2427(03)00007-2
: https://doi.org/10.1016/S0165-2427(03)00007-2

10. Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs

Author:: Lasri, Saadia; Sahibi, Hamid; Natami, Amale; Rhalem, Abdelkebir
Source:: Veterinary immunology and immunopathology 2003 v.91 no.1 pp. 13-18
ISSN:: 0165-2427
Subject:: Leishmania infantum; Western blotting; antibodies; antibody specificity; antigens; cell-mediated immunity; dogs; parasites; proteins
Abstract:: ... Pentamidine treatment has been used successfully to establish immune cellular responses in recovered dogs. In this paper, we examined the appearance and disappearance of antibodies over time against crude Leishmania antigens and purified gp63 or gp70 proteins in sera from cured dogs using a Western blotting technique. Following the treatment, a pattern of antibody specificities to parasite antigen ...
DOI:: 10.1016/S0165-2427(02)00262-3
: https://doi.org/10.1016/S0165-2427(02)00262-3

11. Cloning of porcine scFv antibodies by phage display and expression in Escherichia coli

Author:: Li, Fuqiang; Aitken, Robert
Source:: Veterinary immunology and immunopathology 2004 v.97 no.1-2 pp. 39-51
ISSN:: 0165-2427
Subject:: DNA primers; Escherichia coli; Western blotting; antibodies; bacteria; bacteriophages; complementary DNA; humans; immunoglobulin G; molecular weight; plastics; precipitin tests; swine
Abstract:: ... Oligonucleotide primers were designed for recovery of Ig H, κ and λ transcripts from porcine splenic cDNA. The products were cloned and scFvs constructed in a phage display vector. E. coli HB2151 was transformed with the constructs and upon induction, scFvs of the predicted molecular weight could be detected in culture supernatants by Western blotting and immunoprecipitation with anti-pig IgG. Bac ...
DOI:: 10.1016/j.vetimm.2003.08.011
: https://doi.org/10.1016/j.vetimm.2003.08.011

12. Development of a new monoclonal antibody to ovine chimeric IgE and its detection of systemic and local IgE antibody responses to the intestinal nematode Trichostrongylus colubriformis

Author:: Bendixsen, T.; Windon, R.G.; Huntley, J.F.; MacKellar, A.; Davey, R.J.; McClure, S.J.; Emery, D.L.
Source:: Veterinary immunology and immunopathology 2004 v.97 no.1-2 pp. 11-24
ISSN:: 0165-2427
Subject:: Merino; Trichostrongylus colubriformis; Western blotting; ammonium sulfate; antigens; colostrum; enzyme-linked immunosorbent assay; ewes; genes; immunoglobulin A; immunoglobulin E; immunoglobulin G; immunoglobulin M; jejunum; lambs; larvae; lymph nodes; mast cells; mice; monoclonal antibodies; necropsy; plasma cells; pretreatment; protein binding; tissues
Abstract:: ... The J558L cell line, previously transfected with the ovine Cε gene, was induced to secrete a chimeric IgE protein composed of the ovine heavy chain and a mouse light chain with MW of approximately 80 and 26 kDa, respectively. After purification, the chimeric protein was used to immunise BALB-c mice and monoclonal antibodies (mAbs) were generated. The mAb 2F1, which had greatest anti-IgE activity i ...
DOI:: 10.1016/j.vetimm.2003.08.009
: https://doi.org/10.1016/j.vetimm.2003.08.009

13. Cloning of a gene fragment encoding bovine complement component C3d with expression and characterization of derived fusion proteins

Author:: Firth, M.A.; Moore, D.P.; Pei, Y.; Shewen, P.E.; Lo, R.Y.C.; Yoo, D.; Hodgins, D.C.
Source:: Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 61-71
ISSN:: 0165-2427
Subject:: cattle; food animals; complement; gene expression; protein synthesis; recombinant fusion proteins; genes; B-lymphocytes; antigens; recombinant vaccines; subunit vaccines; Mannheimia haemolytica; leukotoxins; pneumonia; Western blotting; monoclonal antibodies; polyacrylamide gel electrophoresis; mass spectrometry; adjuvants; immunization
Abstract:: ... The gene fragment coding for bovine C3d gene (boC3d) was cloned and expressed as a component of fusion proteins destined for use in vaccine studies in cattle, and for in vitro experiments. This fragment of complement protein C3 (C3d) has been shown to enhance B cell responses when complexed with antigen. Three potential vaccine constructs were engineered to contain one, two or three boC3d units li ...
DOI:: 10.1016/j.vetimm.2006.07.005
: https://doi.org/10.1016/j.vetimm.2006.07.005

14. Toll-like receptor 3 (TLR3): A new marker of canine monocytes-derived dendritic cells (cMo-DC)

Author:: Bonnefont-Rebeix, C.; Marchal, T.; Bernaud, J.; Pin, J.J.; Leroux, C.; Lebecque, S.; Chabanne, L.; Rigal, D.
Source:: Veterinary immunology and immunopathology 2006 v.118 no.1-2 pp. 134-139
ISSN:: 0165-2427
Subject:: cross reaction; disease detection; protein synthesis; Western blotting; monocytes; biomarkers; dendritic cells; dogs; flow cytometry; animal pathogens; monoclonal antibodies; clones; gene expression; disease resistance; molecular cloning; receptors; immunocytochemistry
Abstract:: ... Toll-like receptors (TLRs) are a family of functionally important receptors for recognition of pathogen-associated molecular pattern (PAMP) since they trigger the pro-inflammatory response and upregulation of costimulatory molecules, linking the rapid innate response to adaptative immunity. In human leukocytes, TLR3 has been found to be specifically expressed in dendritic cells (DC). This study ex ...
DOI:: 10.1016/j.vetimm.2007.04.008
: https://doi.org/10.1016/j.vetimm.2007.04.008

15. Bovine lineage-specific gamma delta (γδ) T cell GD3.5 antibody cross-reacts with cell surface antigens on ovine and caprine lymphocytes

Author:: Jones, W.M.; Lexen, R.C.; Burgess, P.L.; Blackburn, S.L.
Source:: Veterinary immunology and immunopathology 2007 v.116 no.3-4 pp. 226-229
ISSN:: 0165-2427
Subject:: surface antigens; cross reaction; protein synthesis; T-lymphocytes; lymphocyte antigens; sheep; cattle; goats; flow cytometry; Western blotting; gene expression; precipitin tests; receptors; genetic markers
Abstract:: ... A specifically defined function for γδ T cells is still a topic of intense debate. Thus, there is great importance in the identification of lineage-specific markers that characterize these cells. Early studies describe a family of lineage-specific cell-surface molecules on ruminant γδ T cells called WC1. More recently, a 220-240 kDa lineage-specific bovine γδ T cell surface marker, GD3.5Ag, was re ...
DOI:: 10.1016/j.vetimm.2007.01.010
: https://doi.org/10.1016/j.vetimm.2007.01.010

16. Cross-reactivity of mAbs to human CD antigens with cells from cattle [Erratum: 2008 Mar. 15, v. 122, issue 1-2, p. 188-189.]

Author:: Sopp, P.; Werling, D.; Baldwin, C.
Source:: Veterinary immunology and immunopathology 2007 v.119 no.1-2 pp. 106-114
ISSN:: 0165-2427
Subject:: cross reaction; interspecific variation; human cell lines; cattle; antibody detection; flow cytometry; Western blotting; monoclonal antibodies; cell differentiation; sequence homology
Abstract:: ... A panel of 377 commercially available mAbs were submitted to the animal homologue section of the 8th International Workshop on Human Leukocyte Differentiation Antigens (HLDA8, Adelaide, Australia) for cross-reactivity studies on different animal species. In this study we describe the results of testing the mAbs on cattle cells by flow cytometry and Western blot. Eight commercial suppliers particip ...
DOI:: 10.1016/j.vetimm.2007.06.014
: https://doi.org/10.1016/j.vetimm.2007.06.014

17. Idiotype expression of IgG1 and IgG2 in dogs naturally infected with Leishmania infantum

Author:: Iniesta, L.; Gállego, M.; Portús, M.
Source:: Veterinary immunology and immunopathology 2007 v.119 no.3-4 pp. 189-197
ISSN:: 0165-2427
Subject:: disease detection; blood serum; serodiagnosis; disease course; dog diseases; biomarkers; dogs; antibody detection; antibody formation; leishmaniasis; Leishmania infantum; Western blotting; immunoglobulin E; signs and symptoms (animals and humans)
Abstract:: ... Here we analyzed, by Western blot analysis, the idiotype expression of IgG1 and IgG2 in 109 canine sera corresponding to 50 dogs from endemic areas of leishmaniosis in order to detect markers related to Leishmania infantum infection and clinical condition (asymptomatic or symptomatic). Twenty-four dogs from an area free of leishmaniosis were used as controls. IgG1 and IgG2 responses in symptomatic ...
DOI:: 10.1016/j.vetimm.2007.05.006
: https://doi.org/10.1016/j.vetimm.2007.05.006

18. Serum amyloid A isoforms in serum and synovial fluid from spontaneously diseased dogs with joint diseases or other conditions

Author:: Kjelgaard-Hansen, M.; Christensen, M.B.; Lee, M.H.; Jensen, A.L.; Jacobsen, S.
Source:: Veterinary immunology and immunopathology 2007 v.117 no.3-4 pp. 296-301
ISSN:: 0165-2427
Subject:: protein synthesis; dog diseases; dogs; protein isoforms; inflammation; Western blotting; joint diseases; immune response; isoelectric focusing; acute phase proteins
Abstract:: ... Serum amyloid A (SAA) is a major acute phase protein in dogs. However, knowledge of qualitative properties of canine SAA and extent of its synthesis in extrahepatic tissues is limited. The aim of the study was to investigate expression of different SAA isoforms in serum and synovial fluid in samples obtained from dogs (n = 16) suffering from different inflammatory or non-inflammatory conditions, w ...
DOI:: 10.1016/j.vetimm.2007.03.008
: https://doi.org/10.1016/j.vetimm.2007.03.008

19. Humoral response to Mycobacterium avium subsp. avium in naturally infected ring-neck doves (Streptopelia risoria)

Author:: Gray, P.L.; Saggese, M.D.; Phalen, D.N.; Tizard, I.
Source:: Veterinary immunology and immunopathology 2008 v.125 no.3-4 pp. 216-224
ISSN:: 0165-2427
Subject:: doves; Streptopelia; bird diseases; mycobacterial diseases; Mycobacterium avium subsp. avium; disease detection; necropsy; screening; serodiagnosis; Western blotting; in vitro culture; Mycobacterium tuberculosis; validity; humoral immunity; wild birds; zoo animals; captive animals
Abstract:: ... Creation of a reliable and easy to use serologic test would greatly improve ante mortem diagnosis of Mycobacterium avium subsp. avium and aid in the control of avian mycobacteriosis, particularly in captive birds. In order to determine whether serodiagnostics could be of value in testing ring-neck doves (Streptopelia risoria) for M. a. avium infection, Western blot analysis was used to assess the ...
DOI:: 10.1016/j.vetimm.2008.05.032
: https://doi.org/10.1016/j.vetimm.2008.05.032

20. Lack of detection of circulating skin-specific IgE autoantibodies in dogs with moderate or severe atopic dermatitis

Author:: Olivry, Thierry; Dunston, Stanley M.; Pluchino, Kristen; Porter, Kyleigh; Hammerberg, Bruce
Source:: Veterinary immunology and immunopathology 2008 v.122 no.1-2 pp. 182-187
ISSN:: 0165-2427
Subject:: dogs; dog diseases; atopic dermatitis; disease diagnosis; fluorescent antibody technique; Western blotting; blood serum; antibody detection; autoantibodies; immunoglobulin E; disease severity; immune response; allergens; biomarkers; validity
Abstract:: ... Human patients with atopic dermatitis (AD) commonly exhibit IgE reactivity to cutaneous self-antigens. The presence of serum IgE autoantibodies appears to correlate with disease severity, and it is suspected to reflect or contribute to tissue damage. The objective of this study was to determine whether IgE autoantibodies specific for cutaneous antigens could be detected in the serum of dogs with A ...
DOI:: 10.1016/j.vetimm.2007.11.003
: https://doi.org/10.1016/j.vetimm.2007.11.003

21. Bovine immunoglobulin E

Author:: Gershwin, Laurel J.
Source:: Veterinary immunology and immunopathology 2009 v.132 no.1 pp. 2-6
ISSN:: 0165-2427
Subject:: cattle diseases; parasitoses; polyclonal antibodies; antibody detection; cattle; vaccines; Western blotting; enzyme-linked immunosorbent assay; monoclonal antibodies; immunoglobulin E; anaphylaxis
Abstract:: ... The recognition of homocytotropic antibodies and their occurrence in response to parasite infection preceded the recognition of IgE as a distinct antibody isotype in the bovine species. Passive cutaneous anaphylaxis was an early reliable and antigen specific method to detect IgE in bovine serum. Yet studies on the role of IgE in bovine disease awaited the production of polyclonal and monoclonal an ...
DOI:: 10.1016/j.vetimm.2009.09.008
PubMed:: 19819026
: https://doi.org/10.1016/j.vetimm.2009.09.008

22. Characterization of the Apa antigen from M. avium subsp. paratuberculosis: A conserved Mycobacterium antigen that elicits a strong humoral response in cattle

Author:: Gioffré, A.; Echeverría-Valencia, G.; Arese, A.; Morsella, C.; Garbaccio, S.; Delgado, F.; Zumárraga, M.; Paolicchi, F.; Cataldi, A.; Romano, M.I.
Source:: Veterinary immunology and immunopathology 2009 v.132 no.2-4 pp. 199-208
ISSN:: 0165-2427
Subject:: cattle diseases; cross reaction; immunoglobulin G; antigens; humoral immunity; microbial genetics; cattle; paratuberculosis; gene expression; Western blotting; enzyme-linked immunosorbent assay; milk; immunization; screening; Mycobacterium avium subsp. paratuberculosis
Abstract:: ... Johne's disease or paratuberculosis is widespread in almost all countries and remains difficult to eradicate. Nowadays, diagnosis of Mycobacterium avium subsp. paratuberculosis (MPTB) infection is one of the main concerns. In this work, we evaluated the expression, biochemical properties and antigenicity of the Apa antigen, encoded by the gene annotated as MAP1569, in the MPTB genome. We confirmed ...
DOI:: 10.1016/j.vetimm.2009.06.008
PubMed:: 19616313
: https://doi.org/10.1016/j.vetimm.2009.06.008

23. Growth-inhibitory effects of four tyrosine kinase inhibitors on neoplastic feline mast cells exhibiting a Kit exon 8 ITD mutation

Author:: Hadzijusufovic, Emir; Peter, Barbara; Rebuzzi, Laura; Baumgartner, Christian; Gleixner, Karoline V.; Gruze, Alexander; Thaiwong, Tuddow; Pickl, Winfried F.; Yuzbasiyan-Gurkan, Vilma; Willmann, Michael; Valent, Peter
Source:: Veterinary immunology and immunopathology 2009 v.132 no.2-4 pp. 243-250
ISSN:: 0165-2427
Subject:: cats; cat diseases; mast cells; spleen; exons; enzyme inhibitors; Western blotting; flow cytometry; apoptosis; mutation
Abstract:: ... Systemic mastocytosis (SM) in felines is a rare neoplasm defined by increased growth and accumulation of immature mast cells (MC) in various organs including the spleen. Although in many cases splenectomy is an effective approach, relapses may occur. In these patients, treatment options are limited. Recent data suggest that various Kit tyrosine kinase inhibitors (TKI) interfere with growth of neop ...
DOI:: 10.1016/j.vetimm.2009.05.007
PubMed:: 19505729
: https://doi.org/10.1016/j.vetimm.2009.05.007

24. Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas

Author:: Muñoz, Fernando A.; Estrada-Parra, Sergio; Romero-Rojas, Andres; Work, Thierry M.; Gonzalez-Ballesteros, Erik; Estrada-Garcia, Iris
Source:: Veterinary immunology and immunopathology 2009 v.131 no.3-4 pp. 211-217
ISSN:: 0165-2427
Subject:: Chelonia mydas; endangered species; T-lymphocytes; cryopreservation; antibodies; antibody detection; thymus gland; immunohistochemistry; flow cytometry; biomarkers; Western blotting; turtles; phenotypic variation; receptors; immunocytochemistry; blood cell counts
Abstract:: ... To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially availab ...
DOI:: 10.1016/j.vetimm.2009.04.015
PubMed:: 19477530
: https://doi.org/10.1016/j.vetimm.2009.04.015

25. Molecular characterization of chicken prion proteins by C-terminal-specific monoclonal antibodies

Author:: Ishiguro, Naotaka; Inoshima, Yasuo; Sassa, Yukiko; Takahashi, Toyomi
Source:: Veterinary immunology and immunopathology 2009 v.128 no.4 pp. 402-406
ISSN:: 0165-2427
Subject:: chickens; interspecific variation; epitopes; protein structure; protein synthesis; recombinant proteins; Western blotting; monoclonal antibodies; genes; mammals; terminal repeat sequences; antigenic variation; animal genetics; prions
Abstract:: ... We obtained seven monoclonal antibodies (mAbs) against chicken cellular prion protein (ChPrPC) by immunizing BALB/c mice with recombinant prion protein (rChPrP). Of the seven mAbs, two mAbs (6 and 26) could recognize rChPrP, but not ChPrPC, in chicken brain lysate via Western blot (WB) analysis. Three C-terminal linear epitopes (AAANQTEVEM, RWWS and SPVPQD) were identified in ChPrP amino acids by ...
DOI:: 10.1016/j.vetimm.2008.11.025
PubMed:: 19118905
: https://doi.org/10.1016/j.vetimm.2008.11.025

26. Secretory expression of porcine interferon-gamma in baculovirus using HBM signal peptide and its inhibition activity on the replication of porcine reproductive and respiratory syndrome virus

Author:: Wang, Yan-Bin; Wang, Zhen-Ya; Chen, Hong-Ying; Cui, Bao-An; Wang, Ya-Bin; Zhang, Hong-Ying; Wang, Rui
Source:: Veterinary immunology and immunopathology 2009 v.132 no.2-4 pp. 314-317
ISSN:: 0165-2427
Subject:: sequence analysis; porcine reproductive and respiratory syndrome; Porcine reproductive and respiratory syndrome virus; interferon-gamma; Baculoviridae; recombinant proteins; signal transduction; cytokines; Western blotting; gene expression; nucleotide sequences; fluorescent antibody technique; swine
Abstract:: ... The gene sequence encoding mature porcine interferon-gamma (PoIFN-γ) fused with a C-terminal 6× histidine tag was cloned into the baculovirus pFastBac™ Dual vector of the Bac-to-Bac Baculovirus expression system under the control of PH promoter. The authentic signal sequence of porcine interferon-gamma was substituted with the honeybee melittin (HBM) signal sequence, and expressed in insect cells. ...
DOI:: 10.1016/j.vetimm.2009.05.017
PubMed:: 19556014
: https://doi.org/10.1016/j.vetimm.2009.05.017

27. A new method to discriminate immunogen-specific heavy-chain homodimer from heterotetramer immunoglobulin G directly in immunized dromedary whole plasma proteins: Western ligand blotting

Author:: Blanc, Michel R.; Anouassi, Abdelhaq; Abed, Muhanad Ahmed; Canépa, Sylvie; Labas, Valérie; Bruneau, Gilles
Source:: Veterinary immunology and immunopathology 2009 v.127 no.3-4 pp. 340-349
ISSN:: 0165-2427
Subject:: infectious diseases; immunoglobulin G; antigen detection; hematologic tests; health status; validity; Western blotting; Camelus dromedarius; immunization; immune response; blood plasma; screening; animal diseases
Abstract:: ... The variable domain of heavy-chain camelid antibodies (VHH), exclusively present in the homodimer IgGs (HC IgG), provides valuable ligands for diagnosis, imaging and therapy. These VHHs are efficiently produced from lymphocytes of immunized animals through phage display and recombination biotechnology. For VHH development it is desirable to identify animals with high affinity HC IgG response by mo ...
DOI:: 10.1016/j.vetimm.2008.10.334
PubMed:: 19091427
: https://doi.org/10.1016/j.vetimm.2008.10.334

28. Characterization of monoclonal antibodies against goat interleukin-18 and their application in the measurement of goat interleukin-18 in LPS-stimulated peripheral blood mononuclear cells by sandwich ELISA

Author:: Cao, Bing-lei; Qiu, Hong-kai; Hu, Jing-dong; Meng, Fan-dong; Zhang, Hong; Zhao, Hong-kun
Source:: Veterinary immunology and immunopathology 2010 v.138 no.3 pp. 235-238
ISSN:: 0165-2427
Subject:: protein binding; hybridomas; lipopolysaccharides; quantitative analysis; interleukins; monocytes; antigens; goats; inflammation; Western blotting; monoclonal antibodies; enzyme-linked immunosorbent assay; immune response; measurement; fluorescent antibody technique
Abstract:: ... In order to develop a specific assay for the measurement of goat IL-18 level, two stable hybridoma cell lines were established which secreted IgG1 monoclonal antibodies (mAbs) against goat IL-18. Specific binding of two mAbs named 2E8 and 4C4 to recombinant goat IL-18 expressed in Escherichia coli was demonstrated in an ELISA and Western blotting. Results also showed that mAbs 2E8 and 4C4 bound to ...
DOI:: 10.1016/j.vetimm.2010.07.005
: https://doi.org/10.1016/j.vetimm.2010.07.005

29. Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays

Author:: Lauber, Beatrice; Marti, Eliane; Plattet, Philippe; Urwyler, Adrian; Rai, Muhammad Farooq; Schmidt, Michael F.G.; Welle, Monika; Zurbriggen, Andreas; Janda, Jozef
Source:: Veterinary immunology and immunopathology 2010 v.137 no.3-4 pp. 310-316
ISSN:: 0165-2427
Subject:: cross reaction; hypersensitivity; dog diseases; parasitoses; dogs; biomarkers; antibodies; allergens; antibody detection; Western blotting; enzyme-linked immunosorbent assay; differential staining; interleukin-4
Abstract:: ... Interleukin 4 (IL-4) plays a central role in immune responses to parasites and allergens. IL-4 drives the differentiation of naive T cells into Th2 cells and regulates immunoglobulin class switching to IgE.Little is known about the role of IL-4 in canine allergies and parasite infections. Most of the information derives from measurement of IL-4 mRNA expression in dog tissues, but detection of IL-4 ...
DOI:: 10.1016/j.vetimm.2010.05.015
: https://doi.org/10.1016/j.vetimm.2010.05.015

30. Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off

Author:: Baravalle, C.; Dallard, B.E.; Ortega, H.H.; Neder, V.E.; Canavesio, V.R.; Calvinho, L.F.
Source:: Veterinary immunology and immunopathology 2010 v.138 no.3 pp. 224-230
ISSN:: 0165-2427
Subject:: dairy cows; temporal variation; messenger RNA; interleukin-1; immunomodulators; lactation; herbal medicines; biochemical pathways; Panax ginseng; reverse transcriptase polymerase chain reaction; Western blotting; gene expression; tumor necrosis factor-alpha; mammary glands; medicinal properties; somatic cell count; udders
Abstract:: ... Biological response modifiers (BRM) are agents that modify the host's response to pathogens with resultant beneficial prophylactic or therapeutic effects. The objectives of this study were to describe the immunomodulatory effects of Panax ginseng (GS) on bovine mammary glands at the end of lactation. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10mL of BRM ( ...
DOI:: 10.1016/j.vetimm.2010.07.019
: https://doi.org/10.1016/j.vetimm.2010.07.019

31. TIR8 receptor expression in bovine tissues

Author:: Riva, F.; Rahman, M.M.; Turin, L.; Ceciliani, F.; Russo, S.; Tribbioli, G.; Lecchi, C.
Source:: Veterinary immunology and immunopathology 2010 v.136 no.1-2 pp. 65-70
ISSN:: 0165-2427
Subject:: cattle; cattle diseases; inflammatory bowel disease; inflammation; animal tissues; gastrointestinal system; Northern blotting; Western blotting; polymerase chain reaction; polyclonal antibodies; messenger RNA; gene expression; receptors
Abstract:: ... The TIR8 receptor (also called SIGIRR) is an orphan member of the TIR superfamily. Its function is still elusive, but it is believed to trigger a negative pathway of regulation of the Toll-like/IL-1 receptor system, crucial for modulating inflammation in gastrointestinal (GI) tract and in other tissues (lung and kidney). The expression pattern of TIR8 in bovine tissues is unknown. Given the import ...
DOI:: 10.1016/j.vetimm.2010.02.009
PubMed:: 20227770
: https://doi.org/10.1016/j.vetimm.2010.02.009

32. Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis

Author:: Giliane S. Souza; Ana Bárbara F. Rodrigues; Andrea Gioffré; Maria I. Romano; Eulógio C.Q. Carvalho; Thatiana L.B. Ventura; Elena B. Lasunskaia
Source:: Veterinary immunology and immunopathology 2011 v.143 no.1-2 pp. 75-82
ISSN:: 0165-2427
Subject:: Mycobacterium avium subsp. paratuberculosis; Western blotting; alanine; antigens; bacteria; cattle; cell culture; fluorescent antibody technique; genes; genomics; immunohistochemistry; monoclonal antibodies; paratuberculosis; pathogens; recombinant proteins; tissue culture; tuberculosis
Abstract:: ... Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of M. avium and M. tuber ...
DOI:: 10.1016/j.vetimm.2011.06.026
PubMed:: 21752474
: https://doi.org/10.1016/j.vetimm.2011.06.026

33. Characterization of antibodies specific for canine TLR4, 5 and 9 by ELISA, Western blotting and immunohistochemistry

Author:: Stephanie Huber; Petra J. Roosje; Jozef Janda; Michaela Schnyder; Thomas W. Jungi; Giuseppe Bertoni; Andreas Zurbriggen; Iwan A. Burgener
Source:: Veterinary immunology and immunopathology 2011 v.144 no.3-4 pp. 247-254
ISSN:: 0165-2427
Subject:: rabbits; Western blotting; mononuclear leukocytes; receptors; interferons; immunity; immunohistochemistry; antibodies; cytokines; enzyme-linked immunosorbent assay; lymph nodes; oxygen; pathogens; cortex; humans; dogs; immunization; nitrogen; inflammation; rodents
Abstract:: ... Toll-like receptors recognize pathogen-associated molecular patterns of microbial origin, and ligand recognition results in the production of different immune mediators such as pro-inflammatory cytokines, interferon, reactive oxygen and nitrogen intermediates, and upregulation of costimmulatory molecules. As these receptors have a critical role in linking pathogen recognition to induction of infla ...
DOI:: 10.1016/j.vetimm.2011.08.025
PubMed:: 21981997
: https://doi.org/10.1016/j.vetimm.2011.08.025

34. Cloning and antibody recognition analysis of the canine 5-lipoxygenase gene

Author:: John P. Loftus; Stewart K. Morgan; Joseph J. Wakshlag
Source:: Veterinary immunology and immunopathology 2011 v.142 no.3-4 pp. 276-279
ISSN:: 0165-2427
Subject:: Western blotting; amino acid sequences; amino acids; antibodies; complementary DNA; conserved sequences; dogs; genes; leukocytes; open reading frames; transfection
Abstract:: ... 5-Lipoxygenase cDNA was prepared from canine white blood cells revealing the full-length message using an oligonucleotide capping method. The sequenced 5-Lipoxygenase open reading frame revealed a 2031 base pair message encoding a 676 amino acid protein. The amino acid sequence showed mild variation with the presumed canine sequence, as well as differences in important residues of known phosphoryl ...
DOI:: 10.1016/j.vetimm.2011.05.006
PubMed:: 21616545
: https://doi.org/10.1016/j.vetimm.2011.05.006

35. Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen–Friedenreich antigen-specific Amaranthus caudatus lectin

Author:: Hideo Akiyoshi; Shunji Sugii; Md. A. Nahid; Katsuhito Sone; Toshiyuki Tanaka; Cao Zheng; Li Yijyun; Mica Aoki; Shigeo Takenaka; Terumasa Shimada; Junichiro Shimizu; Ken-ichi Kiyomiya; Fumihito Ohashi
Source:: Veterinary immunology and immunopathology 2011 v.144 no.3-4 pp. 255-258
ISSN:: 0165-2427
Subject:: Amaranthus caudatus; Western blotting; adrenal glands; antibodies; coatings; cows; dogs; dolphins; enzyme-linked immunosorbent assay; horses; lectins; rabbits; swine
Abstract:: ... The reactivity of different lectins with crude chromogranin A (CgA) obtained from different animals, namely, cow, horse, dog, pig, and dolphin, was examined to identify lectin(s) that would be useful as coating reagent(s) in a sandwich enzyme-linked immunosorbent assay (ELISA). Of the different lectins studied, the Amaranthus caudatus lectin (ACA), which is specific for the Thomsen–Friedenreich (T ...
DOI:: 10.1016/j.vetimm.2011.08.024
PubMed:: 22041600
: https://doi.org/10.1016/j.vetimm.2011.08.024

36. Development of a multiplex assay for the detection of antibodies to Borrelia burgdorferi in horses and its validation using Bayesian and conventional statistical methods

Author:: Bettina Wagner; Heather Freer; Alicia Rollins; Hollis N. Erb; Zhao Lu; Yrjo Gröhn
Source:: Veterinary immunology and immunopathology 2011 v.144 no.3-4 pp. 374-381
ISSN:: 0165-2427
Subject:: ticks; Western blotting; diagnostic specificity; tick bites; detection limit; antibodies; enzyme-linked immunosorbent assay; diagnostic sensitivity; traditional technology; quantitative analysis; antigens; hosts; antibody detection; Borrelia burgdorferi; statistical analysis; Lyme disease; zoonoses; Bayesian theory; surface proteins; horses
Abstract:: ... Lyme disease is a zoonotic, vector-borne disease and occurs in mammals including horses. The disease is induced by infection with spirochetes of the Borrelia burgdorferi sensu lato group. Infection of mammalian hosts requires transmission of spirochetes by infected ticks during tick bites. Lyme disease diagnosis is based on clinical signs, possible exposure to infected ticks, and antibody testing ...
DOI:: 10.1016/j.vetimm.2011.08.005
PubMed:: 21890217
: https://doi.org/10.1016/j.vetimm.2011.08.005

37. Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells

Author:: Akira Matsuda; Akane Tanaka; Yosuke Amagai; Keitaro Ohmori; Sho Nishikawa; Yan Xia; Kaoru Karasawa; Noriko Okamoto; Kumiko Oida; Hyosun Jang; Hiroshi Matsuda
Source:: Veterinary immunology and immunopathology 2011 v.144 no.3-4 pp. 321-328
ISSN:: 0165-2427
Subject:: Western blotting; World Health Organization; clinical examination; dogs; humans; lymphocytes; mast cells; neoplasms; oral administration; polymerase chain reaction; receptors; rodents
Abstract:: ... Glucocorticoid (GC) administration with or without other chemotherapeutic reagents is a commonly used option in the treatment of mast cell malignancies. However, the responsiveness of mast cell tumors to GC treatment varies in individuals, and the regulatory mechanisms determining the GC sensitivity of malignant mast cells remain unclear. Since the expression of the GC receptor (GR) has been repor ...
DOI:: 10.1016/j.vetimm.2011.08.013
PubMed:: 21908058
: https://doi.org/10.1016/j.vetimm.2011.08.013

38. Molecular cloning, in vitro expression and bioactivity of rabbit transforming growth factor-beta receptor type II (rTGF-βRII)

Author:: Haifeng Liu; Xiaohua Wang; Chuntao Wang; Xiaohuan Yuan; Haihua Bao; Xiaobing Fu; Yanhui Chu
Source:: Veterinary immunology and immunopathology 2011 v.140 no.1-2 pp. 55-62
ISSN:: 0165-2427
Subject:: Escherichia coli; Western blotting; amino acid sequences; blood; chickens; collagen; complementary DNA; fibroblasts; host strains; immune system; mice; molecular cloning; molecular weight; open reading frames; polyacrylamide gel electrophoresis; rabbits; rats; receptors; serine; threonine; tissue repair
Abstract:: ... Transforming growth factor-beta receptor II (TGF-βRII) is an attractive target for anti-scarring therapy in wound healing because it attenuates excessive TGF-β which has pleiotropic effects on the immune system. In the present study, the cDNA of rabbit TGF-βRII (rTGF-βRII) was amplified from rabbit peripheral blood by RT-PCR. The open reading frame of rTGF-βRII encodes a protein consisting of 567 ...
DOI:: 10.1016/j.vetimm.2010.11.014
PubMed:: 21167609
: https://doi.org/10.1016/j.vetimm.2010.11.014

39. Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4

Author:: Wataru Ueda; Masanori Tohno; Tomoyuki Shimazu; Hitomi Fujie; Hisashi Aso; Yasushi Kawai; Muneo Numasaki; Tadao Saito; Haruki Kitazawa
Source:: Veterinary immunology and immunopathology 2011 v.143 no.1-2 pp. 148-154
ISSN:: 0165-2427
Subject:: Western blotting; amino acid sequences; amino acids; complementary DNA; digestive tract; esophagus; flow cytometry; immune response; intestinal microorganisms; invertebrates; lymph nodes; molecular cloning; open reading frames; proteins; spleen; swine
Abstract:: ... Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that are present in most invertebrates and vertebrates. Mammals have four PGRPs, PGLYRP1–4. In the present study, we cloned the cDNAs encoding porcine PGLYRP3 and 4 from the esophagus of adult swine. The length of the complete open reading frames of porcine PGLYRP3 and 4 are identical and contain 1125bp encoding 374 amino acid ...
DOI:: 10.1016/j.vetimm.2011.05.026
PubMed:: 21665294
: https://doi.org/10.1016/j.vetimm.2011.05.026

40. Molecular structure, phylogenetic analysis, tissue distribution, and function characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in sheep (Ovis aries)

Author:: Hong-xin Ai; Zhen-zhen Zhang; Yue-fen Shen; Jia-xin Zhang; Xu-ming Zhou; Cui Min; Shan-yuan Zhu; Shuang-quan Zhang
Source:: Veterinary immunology and immunopathology 2011 v.140 no.3-4 pp. 329-334
ISSN:: 0165-2427
Subject:: protein isolates; polyacrylamide gel electrophoresis; phylogeny; Western blotting; genes; cows; mononuclear leukocytes; disulfide bonds; tissue distribution; amino acids; spleen; molecular weight; Escherichia coli; sequence analysis; messenger RNA; antigens; complementary DNA; polymerase chain reaction; amino acid sequences; cDNA libraries; sheep; affinity chromatography
Abstract:: ... Interferon-γ-inducible-lysosomal thiol reductase (GILT) plays a key role in the processing and presentation of MHC class II-restricted antigen (Ag) by catalyzing disulfide bond reduction. In this study, a sheep cDNA, sGILT, encoding GILT protein was isolated from the spleen cDNA library of Ovis aries. It codes for a deduced protein of 244 amino acids with a putative molecular weight of 27.6kDa, wh ...
DOI:: 10.1016/j.vetimm.2011.01.012
PubMed:: 21334075
: https://doi.org/10.1016/j.vetimm.2011.01.012

41. PKC isoenzymes in equine platelets and stimulus induced activation

Author:: Í. Aktan; B. Dunkel; F.M. Cunningham
Source:: Veterinary immunology and immunopathology 2011 v.141 no.3-4 pp. 276-282
ISSN:: 0165-2427
Subject:: platelet-activating factor; Western blotting; protein kinase C; platelet activation; isozymes; horses
Abstract:: ... Protein kinase C (PKC) is an important regulator of platelet activation and different isoenzymes can play positive and negative regulatory roles. The PKC isoenzymes expressed in equine platelets have not been documented but pharmacological inhibition has suggested a role for PKC delta (δ) in modulating responsiveness to platelet activating factor (PAF) (Brooks et al., 2009). Here the PKC isoenzyme ...
DOI:: 10.1016/j.vetimm.2011.03.017
PubMed:: 21513989
: https://doi.org/10.1016/j.vetimm.2011.03.017

42. Selective cloning, characterization, and production of the Culicoides nubeculosus salivary gland allergen repertoire associated with equine insect bite hypersensitivity

Author:: Anna Schaffartzik; Eliane Marti; Sigurbjörg Torsteinsdottir; Philip S. Mellor; Reto Crameri; Claudio Rhyner
Source:: Veterinary immunology and immunopathology 2011 v.139 no.2-4 pp. 200-209
ISSN:: 0165-2427
Subject:: Culicoides nubeculosus; Escherichia coli; Inovirus; Western blotting; allergens; amino acid sequences; bacteriophages; cDNA libraries; clones; complementary DNA; databases; dermatitis; enzyme-linked immunosorbent assay; horses; immediate hypersensitivity; immunoglobulin E; immunotherapy; insects; messenger RNA; molecular weight; open reading frames; pathogenesis; polyacrylamide gel electrophoresis; polymerase chain reaction; salivary glands; skin tests
Abstract:: ... Salivary gland proteins of Culicoides spp. have been suggested to be among the main allergens inducing IgE-mediated insect bite hypersensitivity (IBH), an allergic dermatitis of the horse. The aim of our study was to identify, produce and characterize IgE-binding salivary gland proteins of Culicoides nubeculosus relevant for IBH by phage surface display technology. A cDNA library constructed with ...
DOI:: 10.1016/j.vetimm.2010.10.015
PubMed:: 21071100
: https://doi.org/10.1016/j.vetimm.2010.10.015

43. A fluorescent bead-based multiplex assay for the simultaneous detection of antibodies to B. burgdorferi outer surface proteins in canine serum

Author:: B. Wagner; H. Freer; A. Rollins; H.N. Erb
Source:: Veterinary immunology and immunopathology 2011 v.140 no.3-4 pp. 190-198
ISSN:: 0165-2427
Subject:: Borrelia burgdorferi; Ixodes; Lyme disease; Western blotting; antibodies; antibody detection; antigens; blood proteins; diagnostic sensitivity; diagnostic specificity; dogs; enzyme-linked immunosorbent assay; horses; hosts; probability analysis; recombinant proteins; surface proteins; ticks; vaccination; zoonoses
Abstract:: ... Lyme disease is a zoonotic, vector-borne disease affecting humans, dogs, horses and other species. It is caused by infection with spirochetes of the Borrelia burgdorferi sensu lato group which are transmitted to the mammalian host by infected ticks (Ixodes). Exposure to B. burgdorferi is commonly diagnosed by serological testing. The gold standard for the detection of antibodies to B. burgdorferi ...
DOI:: 10.1016/j.vetimm.2010.12.003
PubMed:: 21208663
: https://doi.org/10.1016/j.vetimm.2010.12.003

44. Adenovirus-based oral vaccine for rabbit hemorrhagic disease

Author:: Xinglong Wang; Li Qiu; Huafang Hao; Weidong Zhang; Xiangjing Fu; Hongling Zhang; Shengfang He; Shuxia Zhang; Enqi Du; Zengqi Yang
Source:: Veterinary immunology and immunopathology 2012 v.145 no.1-2 pp. 277-282
ISSN:: 0165-2427
Subject:: Rabbit hemorrhagic disease virus; Western blotting; antibodies; antigens; biosecurity; coat proteins; immune response; immunization; liver; protein synthesis; rabbit diseases; rabbits; vaccines
Abstract:: ... Vaccine antigens for rabbit hemorrhagic disease virus (RHDV) are currently derived from inactivated RHDV obtained from the livers of experimentally infected rabbits or from several recombinant immunogens. However, the application of these vaccine antigens has been restricted because of biosecurity and immunity characteristics. In the current study, a recombinant adenovirus expressing the RHDV caps ...
DOI:: 10.1016/j.vetimm.2011.11.014
PubMed:: 22154735
: https://doi.org/10.1016/j.vetimm.2011.11.014

45. Binding affinity of anti-xylitol antibodies to canine hepatic vessels

Author:: Akihiro Imai; Toshiho Nishita; Nobutsune Ichihara; Kinji Shirota; Kensuke Orito
Source:: Veterinary immunology and immunopathology 2012 v.149 no.1-2 pp. 108-111
ISSN:: 0165-2427
Subject:: Western blotting; antibodies; binding sites; dogs; hepatic artery; liver; molecular weight; portal vein; rabbits; serum albumin; sugar substitutes; xylitol
Abstract:: ... Xylitol is used as a sugar substitute in food products. Dogs have been reported to experience lethal liver injury after accidental ingestion of xylitol. Because liver injury may be a serious consequence of canine immune-mediated reactions, antibodies produced against xylitol may attack the liver. Therefore, in the present study, we evaluated whether binding sites for xylitol antibodies are located ...
DOI:: 10.1016/j.vetimm.2012.05.016
PubMed:: 22688382
: https://doi.org/10.1016/j.vetimm.2012.05.016

46. Culicoides obsoletus extract relevant for diagnostics of insect bite hypersensitivity in horses

Author:: Nathalie M.A. van der Meide; Chantal Meulenbroeks; Christine van Altena; Anouk Schurink; Bart J. Ducro; Bettina Wagner; Wolfgang Leibold; Jens Rohwer; Frans Jacobs; Marianne M. Sloet van Oldruitenborgh-Oosterbaan; Huub F.J. Savelkoul; Edwin Tijhaar
Source:: Veterinary immunology and immunopathology 2012 v.149 no.3-4 pp. 245-254
ISSN:: 0165-2427
Subject:: Culicoides obsoletus; Western blotting; absorbance; basophils; blood sampling; dermatitis; enzyme-linked immunosorbent assay; histamine; horses; hypersensitivity; immunoglobulin E; insects; probability; probability analysis; Netherlands; North America
Abstract:: ... Insect bite hypersensitivity (IBH) is an allergic dermatitis in horses caused by the bites of Culicoides species. The aim of the present study was to evaluate the applicability of whole body extracts of C. obsoletus (the main species found feeding on horses in the Netherlands), C. nubeculosus (rarely found in The Netherlands) and C. sonorensis (typical for North America) for diagnosis of IBH in ho ...
DOI:: 10.1016/j.vetimm.2012.07.007
PubMed:: 22906994
: https://doi.org/10.1016/j.vetimm.2012.07.007

47. Development and characterization of a monoclonal antibody against porcine CD205

Author:: Lilian Flores-Mendoza; Carlos Velazquez; Jocelyn Bray; Leo Njongmeta; Waithaka Mwangi; Jesús Hernández
Source:: Veterinary immunology and immunopathology 2012 v.146 no.1 pp. 74-80
ISSN:: 0165-2427
Subject:: protein synthesis; dendritic cells; tonsils; vaccines; flow cytometry; Western blotting; monoclonal antibodies; population growth; immune response; receptors; lymph nodes; swine
Abstract:: ... The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognize ...
DOI:: 10.1016/j.vetimm.2012.01.011
PubMed:: 22348805
: https://doi.org/10.1016/j.vetimm.2012.01.011

48. Development of ELISA test for determination of the level of antibodies against Rhodococcus equi in equine serum and colostrum

Author:: Lucjan Witkowski; Jarosław Kaba; Magdalena Rzewuska; Mariusz Nowicki; Olga Szaluś-Jordanow; Jerzy Kita
Source:: Veterinary immunology and immunopathology 2012 v.149 no.3-4 pp. 280-285
ISSN:: 0165-2427
Subject:: Rhodococcus equi; Western blotting; antibodies; antigens; colostrum; diagnostic techniques; enzyme-linked immunosorbent assay; farms; foals; immunoglobulin G; shelf life; vaccination
Abstract:: ... Rhodococcus equi infection occurs worldwide and is one of the major causes of losing foals in the first six months of life. The application of serological tests in the diagnostics of rhodococcosis is limited, however they play a crucial role in immunological studies. The objective of this study was to develop and standardize ELISA test for the determination of the level of antibodies against Rhodo ...
DOI:: 10.1016/j.vetimm.2012.07.009
PubMed:: 22883938
: https://doi.org/10.1016/j.vetimm.2012.07.009

49. Generation of equine TSLP-specific antibodies and their use for detection of TSLP produced by equine keratinocytes and leukocytes

Author:: Jozef Janda; Philippe Plattet; Sigurbjörg Torsteinsdottir; Sigridur Jonsdottir; Andreas Zurbriggen; Eliane Marti
Source:: Veterinary immunology and immunopathology 2012 v.147 no.3-4 pp. 180-186
ISSN:: 0165-2427
Subject:: crosslinking; cross reaction; urticaria; antigen detection; antibody formation; humans; glycosylation; polyclonal antibodies; antibody detection; recombinant proteins; cytokines; Western blotting; enzyme-linked immunosorbent assay; leukocytes; immunoglobulin E; keratinocytes; horse diseases; horses
Abstract:: ... Allergic horses react to innocuous environmental substances by activation of Th2 cells and production of allergen-specific IgE antibodies. The mechanisms leading to Th2 differentiation are not well understood. In humans and mice, epithelial cell-derived thymic stromal lymphopoietin (TSLP) plays a central role in this process. Little is known about equine TSLP (eqTSLP) and its role in allergic dise ...
DOI:: 10.1016/j.vetimm.2012.04.013
: https://doi.org/10.1016/j.vetimm.2012.04.013

50. Immunoprecipitation of equine CD molecules using anti-human MABs previously analyzed by flow cytometry and immunohistochemistry

Author:: Sherif Ibrahim; Falko Steinbach
Source:: Veterinary immunology and immunopathology 2012 v.145 no.1-2 pp. 7-13
ISSN:: 0165-2427
Subject:: Western blotting; antibodies; cross reaction; flow cytometry; horses; humans; immunoglobulin M; immunohistochemistry; lymphocytes; molecular weight; screening
Abstract:: ... Earlier studies investigating the cross-reactivity of antibodies submitted to the HLDA8 had used flow cytometry as a method of choice to screen mAbs for reactivity with equine leukocytes, including two-color flow-cytometry to characterize the lymphocyte population they detect. In addition, immuno-histochemistry (IHC) was used to detect distribution of positive cells in lymphoid tissue sections. In ...
DOI:: 10.1016/j.vetimm.2011.07.021
PubMed:: 22070824
: https://doi.org/10.1016/j.vetimm.2011.07.021

51. Localization of linear B-cell epitopes on goose parvovirus structural protein

Author:: Tian-fei Yu; Bo Ma; Ming-chun Gao; Jun-wei Wang
Source:: Veterinary immunology and immunopathology 2012 v.145 no.1-2 pp. 522-526
ISSN:: 0165-2427
Subject:: epitopes; goslings; diagnostic techniques; Anseriform dependoparvovirus 1; glutathione transferase; peptides; Western blotting; structural proteins; amino acids; ducklings; single-stranded DNA; Escherichia coli
Abstract:: ... Goose parvovirus (GPV), a small non-enveloped ssDNA virus, can cause Derzsy's disease, a highly contagious and lethal disease in goslings and muscovy ducklings, leading to a huge economic loss. However, little is known about the localization of B-cell epitopes on GPV structural protein. To address the issue, the structural protein of GPV was dissected into sets of partially overlapping fragments a ...
DOI:: 10.1016/j.vetimm.2011.11.022
PubMed:: 22209204
: https://doi.org/10.1016/j.vetimm.2011.11.022

52. Monoclonal antibodies recognizing mucus immunoglobulin and surface immunoglobulin-positive cells of flounder (Paralichthys olivaceus)

Author:: Xiu-zhen Sheng; Guo-jing Xu; Xiao-qian Tang; Wen-bin Zhan
Source:: Veterinary immunology and immunopathology 2012 v.145 no.1-2 pp. 143-150
ISSN:: 0165-2427
Subject:: Paralichthys olivaceus; Western blotting; agarose; chromatography; enzyme-linked immunosorbent assay; filtration; flounder; flow cytometry; gels; gills; immunoglobulin M; lymphocytes; mice; molecular weight; monoclonal antibodies; mucosal immunity; mucus; polyacrylamide gel electrophoresis; spleen
Abstract:: ... Mucus immunoglobulin (Ig) of flounder (Paralichthys olivaceus) was purified by the combination of salting-out, Sephacryl S-300 gel filtration chromatography and DEAE Sepharose chromatography. According to the SDS-PAGE and native-PAGE, the purified mucus Ig showed apparent molecular weights of 72kDa (heavy chain) and 26kDa (light chain), and a total molecular weight of 798kDa, which indicated mucus ...
DOI:: 10.1016/j.vetimm.2011.10.022
PubMed:: 22209367
: https://doi.org/10.1016/j.vetimm.2011.10.022

53. Monoclonal antibodies to equine CD23 identify the low-affinity receptor for IgE on subpopulations of IgM⁺ and IgG1⁺ B-cells in horses

Author:: Bettina Wagner; Julia M. Hillegas; Susanna Babasyan
Source:: Veterinary immunology and immunopathology 2012 v.146 no.2 pp. 125-134
ISSN:: 0165-2427
Subject:: T-lymphocytes; Western blotting; antigen presentation; flow cytometry; horse diseases; horses; immune response; immunoglobulin E; monoclonal antibodies; monocytes; receptors; recombinant fusion proteins
Abstract:: ... CD23, also called FcɛRII, is the low-affinity receptor for IgE and has first been described as a major receptor regulating IgE responses. In addition, CD23 also binds to CD21, integrins and MHC class II molecules and thus has a much wider functional role in immune regulation ranging from involvement in antigen-presentation to multiple cytokine-like functions of soluble CD23. The role of CD23 durin ...
DOI:: 10.1016/j.vetimm.2012.02.007
PubMed:: 22405681
: https://doi.org/10.1016/j.vetimm.2012.02.007

54. Novel link between inflammation and impaired glucose transport during equine insulin resistance

Author:: A.P. Waller; L. Huettner; K. Kohler; V.A. Lacombe
Source:: Veterinary immunology and immunopathology 2012 v.149 no.3-4 pp. 208-215
ISSN:: 0165-2427
Subject:: Western blotting; adipose tissue; biopsy; glucose; glucose tolerance; glucose transporters; horses; immune response; inflammation; insulin; insulin resistance; muscle tissues; pathogenesis; pathophysiology; phosphorylation; protein synthesis; receptors; tumor necrosis factors
Abstract:: ... Although insulin resistance (IR) has been increasingly recognized in horses, a clear understanding of its pathophysiology is lacking. The purpose of the present study was to determine the early pathologic changes in IR horses by characterizing alterations in proteins that play key roles in innate immunological responses and inflammatory pathways, and by identifying potential links with glucose tra ...
DOI:: 10.1016/j.vetimm.2012.07.003
PubMed:: 22871576
: https://doi.org/10.1016/j.vetimm.2012.07.003

55. Proteomic alteration of Marc-145 cells and PAMs after infection by porcine reproductive and respiratory syndrome virus

Author:: Zhuang Ding; Zhi-jie Li; Xiao-dong Zhang; Ya-gang Li; Chang-jun Liu; Yan-Ping Zhang; Yang Li
Source:: Veterinary immunology and immunopathology 2012 v.145 no.1-2 pp. 206-213
ISSN:: 0165-2427
Subject:: Porcine reproductive and respiratory syndrome virus; Western blotting; electrophoresis; gels; hosts; macrophages; messenger RNA; pathogenesis; protein synthesis; proteins; proteome; proteomics
Abstract:: ... Viral infections usually result in alterations in the host cell proteome, which determine the fate of infected cells and the progress of pathogenesis. To uncover cellular protein responses in porcine reproductive and respiratory syndrome virus (PRRSV), infected pulmonary alveolar macrophages (PAMs) and Marc-145 cells were subjected to proteomic analysis involving two-dimensional electrophoresis (2 ...
DOI:: 10.1016/j.vetimm.2011.11.005
PubMed:: 22137209
: https://doi.org/10.1016/j.vetimm.2011.11.005

56. Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination

Author:: Zhengliang Ou-yang; Peiran Wang; Youhua Huang; Xiaohong Huang; Qingjiao Wan; Sheng Zhou; Jingguang Wei; Yongcan Zhou; Qiwei Qin
Source:: Veterinary immunology and immunopathology 2012 v.149 no.1-2 pp. 38-45
ISSN:: 0165-2427
Subject:: DNA; Epinephelus akaara; Iridovirus; Western blotting; antigens; bioinformatics; grouper; immunity; molecular cloning; open reading frames; protective effect; rapid methods; recombinant proteins; recombinant vaccines; splenocytes; survival rate; vaccination; Singapore
Abstract:: ... In this study, we described a rapid and efficient method which integrated the bioinformatic prediction and DNA vaccine technology to identify vaccine candidates against Singapore grouper iridovirus (SGIV). The 162 previously defined open reading frames (ORFs) of SGIV were subjected to extensive sequence similarity searches, as well as motif, cellular location, and domain prediction. Based on our a ...
DOI:: 10.1016/j.vetimm.2012.05.021
PubMed:: 22698389
: https://doi.org/10.1016/j.vetimm.2012.05.021

57. Characterization of the circulating serum amyloid A in bottlenose dolphins

Author:: Takao Segawa; Toru Otsuka; Takuya Itou; Miwa Suzuki; Nana Karatani; Takeo Sakai
Source:: Veterinary immunology and immunopathology 2013 v.152 no.3-4 pp. 218-224
ISSN:: 0165-2427
Subject:: Tursiops truncatus; Western blotting; amino acid sequences; amyloid; dolphins; inflammation; isoelectric focusing; isoelectric point; liver; messenger RNA; swine
Abstract:: ... Several isoforms of serum amyloid A (SAA) have been identified so far and because the plasma concentration of it increases dramatically, it is used as an indicator of inflammation in animals. In many terrestrial mammals, the circulating isoforms are SAA1 and SAA2, which are synthesized in the liver. Extra-hepatically synthesized SAA3, however, is a predominantly local SAA isoform with a characteri ...
DOI:: 10.1016/j.vetimm.2012.12.006
PubMed:: 23333194
: https://doi.org/10.1016/j.vetimm.2012.12.006

58. Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)

Author:: Victoria A. Valdenegro-Vega; Philip Crosbie; Benita Vincent; Kenneth D. Cain; Barbara F. Nowak
Source:: Veterinary immunology and immunopathology 2013 v.151 no.1-2 pp. 113-123
ISSN:: 0165-2427
Subject:: Salmo salar; Western blotting; antibodies; antibody formation; antigens; enzyme-linked immunosorbent assay; explants; fish; fluorescein; immunization; mucus
Abstract:: ... This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens – dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) – administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post ...
DOI:: 10.1016/j.vetimm.2012.10.010
PubMed:: 23206403
: https://doi.org/10.1016/j.vetimm.2012.10.010

59. Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines

Author:: C.L. Schnabel; S. Wagner; B. Wagner; M.C. Durán; S. Babasyan; I. Nolte; C. Pfarrer; K. Feige; H. Murua Escobar; J.-M.V. Cavalleri
Source:: Veterinary immunology and immunopathology 2013 v.156 no.1-2 pp. 1-19
ISSN:: 0165-2427
Subject:: Western blotting; cross reaction; flow cytometry; granulocyte-macrophage colony-stimulating factor; horses; immunohistochemistry; interleukin-12; interleukin-18; interleukin-6; interleukin-8; macrophages; messenger RNA; monoclonal antibodies; mononuclear leukocytes; swine
Abstract:: ... Research on equine cytokines is often performed by analyses of mRNA. For many equine cytokines an analysis on the actual protein level is limited by the availability of antibodies against the targeted cytokines. Generation of new antibodies is ongoing but time consuming. Thus, testing the reactivity of commercially available antibodies for cross-reactivity with equine cytokines is of particular in ...
DOI:: 10.1016/j.vetimm.2013.09.012
PubMed:: 24139393
: https://doi.org/10.1016/j.vetimm.2013.09.012

60. Immunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer's disease in pigs

Author:: Sonia Martínez-Martínez; Rafael Frandoloso; Elías F. Rodríguez Ferri; Concha Gil; Carolina Hernández-Haro; Sheila Yubero; César B. Gutiérrez Martín
Source:: Veterinary immunology and immunopathology 2013 v.151 no.3-4 pp. 235-247
ISSN:: 0165-2427
Subject:: Haemophilus parasuis; Western blotting; antibodies; antigens; electrophoresis; gels; glyceraldehyde 3-phosphate; immunization; immunoglobulin A; immunoglobulin G; immunoglobulin M; peptidylprolyl isomerase; serotypes; sialidase; subunit vaccines; swine; swine diseases; transferrin
Abstract:: ... An immunoproteomic analysis of the protective response of subunit and commercial vaccines in colostrum-deprived pigs against Glässer's disease was carried out. A mixture of proteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasaki strain (serovar 5) was inoculated intramuscularly (PAPTM) and intratracheally (PAPTCp), along with a commercial bacterin. PAPT were separat ...
DOI:: 10.1016/j.vetimm.2012.11.014
PubMed:: 23266097
: https://doi.org/10.1016/j.vetimm.2012.11.014

61. Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells

Author:: Yunhe Fu; Bo Liu; Xiaosheng Feng; Zhicheng Liu; Dejie Liang; Fengyang Li; Depeng Li; Yongguo Cao; Shuang Feng; Xichen Zhang; Naisheng Zhang; Zhengtao Yang
Source:: Veterinary immunology and immunopathology 2013 v.151 no.1-2 pp. 20-27
ISSN:: 0165-2427
Subject:: Escherichia coli; Gram-negative bacteria; Western blotting; cattle; endometrium; epithelial cells; immunity; lipopolysaccharides; polymerase chain reaction; receptors; transcription factors
Abstract:: ... The endometrium is easily contaminated with bacteria and the endometrial epithelial cells (EECs) play an important role in defence against invading pathogens which recognized pathogen-associated molecular patterns (PAMPs) via pattern recognition receptors (PRRs). Toll-like receptor 4 (TLR4) can recognize lipopolysaccharide (LPS) from Gram-negative bacteria and initiates innate immune responses. In ...
DOI:: 10.1016/j.vetimm.2012.09.039
PubMed:: 23218938
: https://doi.org/10.1016/j.vetimm.2012.09.039

62. Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes

Author:: Shingo Maeda; Sadatoshi Maeda; Koichi Ohno; Noriyuki Kaji; Masatoshi Hori; Yasuhito Fujino; Hajime Tsujimoto
Source:: Veterinary immunology and immunopathology 2013 v.153 no.1-2 pp. 17-25
ISSN:: 0165-2427
Subject:: granulocyte-macrophage colony-stimulating factor; Western blotting; receptors; trypsin; gene expression; tumor necrosis factors; interleukin-8; messenger RNA; humans; dogs; proteolysis; keratinocytes; fluorescent antibody technique; fluorescent dyes; allergenicity; inflammation; allergens; atopic dermatitis; agonists
Abstract:: ... Although the molecular basis of the allergenicity remains to be fully elucidated, the ability of allergens to elicit allergic responses is at least partly attributed to their proteolytic activity. Protease-activated receptor-2 (PAR-2) is a G protein-coupled receptor that is activated by site-specific proteolysis by serine proteases and is known to mediate inflammatory processes in various tissues. ...
DOI:: 10.1016/j.vetimm.2013.01.018
PubMed:: 23465358
: https://doi.org/10.1016/j.vetimm.2013.01.018

63. Recombinant adenovirus expressing F and H fusion proteins of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in goats

Author:: Yong Wang; Guangqing Liu; Zongyan Chen; Chuanfeng Li; Lijun Shi; Wenchao Li; Huaxin Huang; Chunai Tao; Chaofei Cheng; Binrui Xu; Gang Li
Source:: Veterinary immunology and immunopathology 2013 v.154 no.1-2 pp. 1-7
ISSN:: 0165-2427
Subject:: Adenoviridae; Western blotting; acute course; antibodies; fluorescent antibody technique; glycoproteins; goats; hosts; humoral immunity; immune response; immunization; lymphocyte proliferation; neutralization; peste des petits ruminants; seroconversion; vaccines; viruses
Abstract:: ... Peste des petits ruminants (PPR) is an acute and contagious disease of some small ruminants caused by peste des petits ruminants virus (PPRV). Fusion (F) protein and hemagglutinin (H) protein are two glycoproteins of PPRV that might induce a protective immune response. In this study, three replication-defective recombinant adenoviruses were constructed and the immunogenicity was evaluated in goats ...
DOI:: 10.1016/j.vetimm.2013.05.002
PubMed:: 23707075
: https://doi.org/10.1016/j.vetimm.2013.05.002

64. Anti-lymphoproliferative activity of alpha-2-macroglobulin in the plasma of hibernating 13-lined ground squirrels and woodchucks

Author:: Donna G. Sieckmann; Howard Jaffe; Susanne Golech; DeCheng Cai; John M. Hallenbeck; Richard M. McCarron
Source:: Veterinary immunology and immunopathology 2014 v.161 no.1-2 pp. 1-11
ISSN:: 0165-2427
Subject:: Marmota monax; Western blotting; cell culture; cold; concanavalin A; gel chromatography; hibernation; high performance liquid chromatography; humans; mass spectrometry; mice; molecular weight; spleen; squirrels
Abstract:: ... Plasma from hibernating (HIB) woodchucks (Marmota monax) or 13-lined ground squirrels (Ictidomys tridecemlineatus) suppressed 3H-thymidine uptake in mouse spleen cell cultures stimulated with Concanavalin A (ConA); plasma from non-hibernating animals were only slightly inhibitory. Maximum inhibition occurred when HIB plasma was added to the cultures prior to ConA. After HPLC size exclusion chromat ...
DOI:: 10.1016/j.vetimm.2014.05.006
: https://doi.org/10.1016/j.vetimm.2014.05.006

65. Characterization of arginase expression by equine neutrophils

Author:: Anouk Lavoie-Lamoureux; James G. Martin; Jean-Pierre Lavoie
Source:: Veterinary immunology and immunopathology 2014 v.157 no.3-4 pp. 206-213
ISSN:: 0165-2427
Subject:: Western blotting; arginase; enzyme activity; gene expression; horses; interleukin-4; messenger RNA; metabolism; neutrophils; ornithine decarboxylase; ornithine-oxo-acid transaminase; polymerase chain reaction
Abstract:: ... Neutrophils are the predominant cells recruited in the airways of horses suffering from heaves. These cells have been shown to express arginase in some species. The metabolism of l-arginine is thought to be involved in chronic inflammation, and airway obstruction and remodeling. The aim of this study was to assess the expression, regulation, activity, and functional role of arginase isoforms in eq ...
DOI:: 10.1016/j.vetimm.2013.12.007
: https://doi.org/10.1016/j.vetimm.2013.12.007

66. Egg yolk IgY against RHDV capsid protein VP60 promotes rabbit defense against RHDV infection

Author:: Zai Xin Li; Wei Dong Hu; Bing Chao Li; Tian You Li; Xiao Yang Zhou; Zhi Zhang
Source:: Veterinary immunology and immunopathology 2014 v.157 no.1-2 pp. 97-104
ISSN:: 0165-2427
Subject:: Escherichia coli; Rabbit hemorrhagic disease virus; Western blotting; antigens; antiserum; capsid; chickens; chromatography; egg yolk; enzyme-linked immunosorbent assay; immune response; immunoglobulin Y; plasmids; rabbits; therapeutics; viruses
Abstract:: ... VP60 capsid protein is the major structural and immunogenicity protein of RHDV (Rabbit hemorrhagic disease virus, RHDV), and has been implicated as a main protein antigen in RHDV diagnosis and vaccine design. In this report, egg yolk antibody (IgY) against N-terminal of VP60 was evaluated and developed as a new strategy for RHDV therapy. Briefly, N-terminal of VP60 (∼250aa) fragment was cloned and ...
DOI:: 10.1016/j.vetimm.2013.10.002
: https://doi.org/10.1016/j.vetimm.2013.10.002

67. Immunoproteomics of Brucella abortus RB51 as candidate antigens in serological diagnosis of brucellosis

Author:: Ji-Yeon Kim; So-Ra Sung; Kichan Lee; Hyang-Keun Lee; Sung-Il Kang; Jin Ju Lee; Suk Chan Jung; Yong Ho Park; Moon Her
Source:: Veterinary immunology and immunopathology 2014 v.160 no.3-4 pp. 218-224
ISSN:: 0165-2427
Subject:: Brucella melitensis biovar Abortus; Escherichia coli; Gram-negative bacteria; Western blotting; Yersinia enterocolitica; aldehyde dehydrogenase; antibodies; antigens; antiserum; brucellosis; cattle; cell division; chaperonins; cross reaction; lipopolysaccharides; mutants; pH; serodiagnosis; superoxide dismutase; two-dimensional gel electrophoresis
Abstract:: ... The current brucellosis serodiagnostic assays are chiefly based on detecting anti-LPS (lipopolysaccharide) antibodies. However, cross-reaction with some gram-negative bacteria can occasionally induce due to similar O-polysaccharide (OPS) structure. Therefore, the aim of the present study was to identify new candidate antigens from Brucella abortus RB51, a mutant strain lacking the LPS portion, whi ...
DOI:: 10.1016/j.vetimm.2014.05.009
: https://doi.org/10.1016/j.vetimm.2014.05.009

68. A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

Author:: Yanina P. Hecker; Verónica Cóceres; Silvina E. Wilkowsky; José M. Jaramillo Ortiz; Eleonora L. Morrell; Andrea E. Verna; Agustina Ganuza; Dora B. Cano; Lilian Lischinsky; Sergio O. Ángel; Patricia Zamorano; Anselmo C. Odeón; María R. Leunda; Carlos M. Campero; Bror Morein; Dadín P. Moore
Source:: Veterinary immunology and immunopathology 2014 v.162 no.3-4 pp. 142-153
ISSN:: 0165-2427
Subject:: Escherichia coli; Neospora caninum; Western blotting; antibodies; antigens; fetus; heifers; immune response; immunization; immunohistochemistry; interferon-gamma; intravenous injection; nickel; polymerase chain reaction; pregnancy; protein synthesis; recombinant proteins; transplacental transmission; vaccines
Abstract:: ... The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20 and rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of Neospora caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were ...
DOI:: 10.1016/j.vetimm.2014.11.001
: https://doi.org/10.1016/j.vetimm.2014.11.001

69. TLR4 is constitutively expressed in chick thymic epithelial cells

Author:: Hai-Bo Huang; Quan-Hang Xiang; Hui Wu; Abdur Rahman Ansari; Le Wen; Xiao-Hong Ge; Ji-Xiang Wang; Ke-Mei Peng; Hua-Zhen Liu
Source:: Veterinary immunology and immunopathology 2014 v.158 no.3-4 pp. 182-188
ISSN:: 0165-2427
Subject:: Toll-like receptor 4; Western blotting; chicks; epithelial cells; gene expression; gilts; immunohistochemistry; juveniles; messenger RNA; protein synthesis; quantitative polymerase chain reaction; thymus gland
Abstract:: ... Toll-like receptor 4 (TLR4) has been suggested to play a regulatory role in immune cell development; however, studies regarding the role of TLR4 in the development of the chick thymus are scarce. In this study, we investigated the distribution and expression pattern of TLR4 in normal chick thymi at different stages of development, in order to better understand the role of TLR4 in chick thymus deve ...
DOI:: 10.1016/j.vetimm.2014.01.005
: https://doi.org/10.1016/j.vetimm.2014.01.005

70. Toll-like receptor and pro-inflammatory cytokine expression during prolonged hyperinsulinaemia in horses: Implications for laminitis

Author:: M.A. de Laat; C.K. Clement; C.M. McGowan; M.N. Sillence; C.C. Pollitt; V.A. Lacombe
Source:: Veterinary immunology and immunopathology 2014 v.157 no.1-2 pp. 78-86
ISSN:: 0165-2427
Subject:: Standardbred; Western blotting; glucose; hooves; horses; hyperinsulinemia; interleukin-6; laminitis; muscle protein; protein synthesis; skeletal muscle; tumor necrosis factor-alpha
Abstract:: ... Equine laminitis, a disease of the lamellar structure of the horse's hoof, can be incited by numerous factors that include inflammatory and metabolic aetiologies. However, the role of inflammation in hyperinsulinaemic laminitis has not been adequately defined. Toll-like receptor (TLR) activation results in up-regulation of inflammatory pathways and the release of pro-inflammatory cytokines, includ ...
DOI:: 10.1016/j.vetimm.2013.10.010
: https://doi.org/10.1016/j.vetimm.2013.10.010

71. Cell-penetrating peptide-mediated subunit vaccine generates a potent immune response and protection against Streptococcus iniae in Japanese flounder (Paralichthys olivaceus)

Author:: Yun Sun; Yong-hua Hu
Source:: Veterinary immunology and immunopathology 2015 v.167 no.3-4 pp. 96-103
ISSN:: 0165-2427
Subject:: immunoglobulin M; Western blotting; antigen-presenting cells; cell membranes; vaccination; Streptococcus iniae; macrophage activation; antibodies; lymphocytes; recombinant fusion proteins; gene expression; transactivators; gene expression regulation; subunit vaccines; Drosophila; interleukin-8; Paralichthys olivaceus; blood serum; Human immunodeficiency virus 1; antigens; flounder; homeodomain proteins; transcription (genetics); tumor necrosis factor-alpha; kidneys; bacterial vaccines; interleukin-1beta; survival rate; protein synthesis; fluorescence microscopy
Abstract:: ... The efficiency of antigen capture, processing, and presentation by antigen-presenting cells is the key to induce an effective immune response. Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargoes and have an attractive potential for vaccine delivery. In this study, the Drosophila Antennapedia homeoprotein (Antp) and the human immunodefici ...
DOI:: 10.1016/j.vetimm.2015.07.008
: https://doi.org/10.1016/j.vetimm.2015.07.008

72. Construction of Eimeria tenella multi-epitope DNA vaccines and their protective efficacies against experimental infection

Author:: Xiaokai Song; Lixin Xu; Ruofeng Yan; Xinmei Huang; Xiangrui Li
Source:: Veterinary immunology and immunopathology 2015 v.166 no.3-4 pp. 79-87
ISSN:: 0165-2427
Subject:: Eimeria tenella; T-lymphocytes; Western blotting; adjuvants; animal experimentation; body weight changes; chickens; coccidiosis; coccidiostats; epitopes; genes; immune response; interferon-gamma; interleukin-2; laboratory animals; merozoites; oocysts; parasites; recombinant vaccines; reverse transcriptase polymerase chain reaction; survival rate; translation (genetics); vaccine development
Abstract:: ... The search for effective vaccines against chicken coccidiosis remains a challenge because of the complex organisms with multiple life cycle stages of Eimeria. Combination of T-cell epitopes from different stages of Eimeria life cycle could be an optimal strategy to overcome the antigen complexity of the parasite. In this study, 4 fragments with concentrated T-cell epitopes from the sporozoite anti ...
DOI:: 10.1016/j.vetimm.2015.05.005
: https://doi.org/10.1016/j.vetimm.2015.05.005

73. Development and characterization of monoclonal antibodies against goose CD3ɛ extracellular domain and their application in detection of CD3+ T lymphocytes

Author:: Xuelian Zhang; Jianwei Shao; Chunqiu Cao; Bo Ma; Junwei Wang
Source:: Veterinary immunology and immunopathology 2015 v.168 no.3-4 pp. 135-139
ISSN:: 0165-2427
Subject:: T-lymphocytes; Western blotting; antigens; geese; monoclonal antibodies; recombinant proteins
Abstract:: ... CD3 is one of the most important cell surface markers of T lymphocytes, and has an important function in signal transmission during antigen recognition. In this study, monoclonal antibodies against goose CD3ɛ extracellular domain were developed and characterized for the first time. The purified recombinant goose CD3ɛ extracellular domain protein and pcDNA3.1-GoCD3ɛex were used as immunogens to dev ...
DOI:: 10.1016/j.vetimm.2015.10.012
: https://doi.org/10.1016/j.vetimm.2015.10.012

74. Full-length cDNA cloning, molecular characterization and differential expression analysis of peroxiredoxin 6 from Ovis aries

Author:: Nan-Nan Liu; Zeng-Shan Liu; Shi-Ying Lu; Pan Hu; Yan-Song Li; Xiao-Li Feng; Shou-Yin Zhang; Nan Wang; Qing-Feng Meng; Yong-Jie Yang; Feng Tang; Yun-Ming Xu; Wen-Hui Zhang; Xing Guo; Xiao-Feng Chen; Yu Zhou; Hong-Lin Ren
Source:: Veterinary immunology and immunopathology 2015 v.164 no.3-4 pp. 208-219
ISSN:: 0165-2427
Subject:: phospholipase A2; reactive oxygen species; Western blotting; Brucella melitensis; heart; open reading frames; biomarkers; recombinant proteins; rapid amplification of cDNA ends; leukocytes; monoclonal antibodies; amino acids; intestines; spleen; homeostasis; stomach; molecular weight; lymph nodes; gene expression regulation; tissues; virulence; messenger RNA; complementary DNA; peroxiredoxin; muscles; immune response; kidneys; brucellosis; protein synthesis; liver; sheep; vaccines; glutathione peroxidase
Abstract:: ... Peroxiredoxin 6 (Prdx6), an important antioxidant enzyme that can eliminate reactive oxygen species (ROS) to maintain homeostasis, is a bifunctional protein that possesses the activities of both glutathione peroxidase and phospholipase A2. In this study, a novel full-length Prdx6 cDNA (OaPrdx6) was cloned from Sheep (Ovis aries) using rapid amplification of cDNA ends (RACE). The full-length cDNA o ...
DOI:: 10.1016/j.vetimm.2015.01.006
: https://doi.org/10.1016/j.vetimm.2015.01.006

75. Novel monoclonal antibody against alphaX subunit from horse CD11c/CD18 integrin

Author:: Gerardo Pavel Espino-Solis; Veronica Quintero-Hernandez; Alejandro Olvera-Rodriguez; Juana Calderon-Amador; Martha Pedraza-Escalona; Alexei Licea-Navarro; Leopoldo Flores-Romo; Lourival Domingos Possani
Source:: Veterinary immunology and immunopathology 2015 v.164 no.3-4 pp. 220-226
ISSN:: 0165-2427
Subject:: Escherichia coli; Western blotting; dendritic cells; enzyme-linked immunosorbent assay; epitopes; horses; immunohistochemistry; integrins; lymph nodes; mice; monoclonal antibodies; mononuclear leukocytes; vaccines
Abstract:: ... The αX I-domain of the horse integrin CD11c was successfully expressed in Escherichia coli, purified, biochemically characterized and used as immunogen to generate murine monoclonal antibodies against horse CD11c, which are not yet commercially available. One monoclonal antibody mAb-1C4 against the αX I-domain, is an IgG2a able to interact with the recombinant I-domain, showing an EC50=2.4ng accor ...
DOI:: 10.1016/j.vetimm.2015.02.002
: https://doi.org/10.1016/j.vetimm.2015.02.002

76. Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus

Author:: Yingqi Zhu; Chuanfeng Li; Zhuangli Bi; Zongyan Chen; Chunchun Meng; Gui-Jun Wang; Chan Ding; Guangqing Liu
Source:: Veterinary immunology and immunopathology 2015 v.165 no.1-2 pp. 88-92
ISSN:: 0165-2427
Subject:: Reoviridae; Semliki Forest virus; Western blotting; ducklings; fluorescent antibody technique; genes; immune response; interferon-gamma; interleukin-4; neutralization; neutralizing antibodies; recombinant vaccines; replicon; vaccine development
Abstract:: ... A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV). The sigma C gene of NDRV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. After transfected into BHK-21 cells, the antigenicity of the sigma C protein was confirmed using an indirect immunofluorescence and Western blot assay. ...
DOI:: 10.1016/j.vetimm.2015.03.001
: https://doi.org/10.1016/j.vetimm.2015.03.001

77. Toll-like receptor 10 expression in chicken, cattle, pig, dog, and rat lungs

Author:: Yadu Balachandran; Steven Knaus; Sarah Caldwell; Baljit Singh
Source:: Veterinary immunology and immunopathology 2015 v.168 no.3-4 pp. 184-192
ISSN:: 0165-2427
Subject:: Escherichia coli; Toll-like receptor 10; Western blotting; antibodies; cattle; chickens; dogs; endothelium; human diseases; innate immunity; lipopolysaccharides; lungs; molecular weight; muscles; neutrophils; plasma membrane; rats; swine
Abstract:: ... Toll-like receptors (TLRs) are conserved immune receptors that play critical roles in innate immunity and are known as “gate keepers” of immune system. TLR10 is identified as type 1 plasma membrane protein but the identity of its ligand remains unclear. Till date, no data are available on tissue and cell specific expression of TLR10 in normal and inflamed lungs of domestic animal species, and rat, ...
DOI:: 10.1016/j.vetimm.2015.09.007
: https://doi.org/10.1016/j.vetimm.2015.09.007

78. The localization and differential expression of Serum Amyloid A in bovine liver and adipose tissue depots

Author:: Fabrizio Ceciliani; Laura Soler; Guido Grilli; Andreia T. Marques; Chiara Giudice; Cristina Lecchi
Source:: Veterinary immunology and immunopathology 2015 v.168 no.1-2 pp. 35-39
ISSN:: 0165-2427
Subject:: Western blotting; acute phase proteins; adipocytes; adipose tissue; amyloid; blood serum; blood vessels; cattle; endothelial cells; gene expression; gene expression regulation; hepatocytes; immunohistochemistry; liver; messenger RNA; parenchyma (animal tissue); polyclonal antibodies; polymerase chain reaction; staining; withers
Abstract:: ... In this article the localization of the acute phase protein Serum Amyloid A (SAA) in different depots of bovine adipose tissue (AT) and liver is reported. Quantitative (Real Time) PCR was paired to immunohistochemistry after the production of a specific polyclonal antibody. SAA's mRNA was found in all analyzed AT depots included in the present study, the AT located in the withers being the major s ...
DOI:: 10.1016/j.vetimm.2015.08.004
: https://doi.org/10.1016/j.vetimm.2015.08.004

79. Demonstration of the ability of a canine Lyme vaccine to reduce the incidence of histological synovial lesions following experimentally-induced canine Lyme borreliosis

Author:: Deborah A. Grosenbaugh; Daniel R. Rissi; Paula M. Krimer
Source:: Veterinary immunology and immunopathology 2016 v.180 pp. 29-33
ISSN:: 0165-2427
Subject:: Beagle; Borrelia burgdorferi; Ixodes scapularis; Lyme disease; Western blotting; antigens; biopsy; disease vectors; dog diseases; dogs; histopathology; lameness; models; polymerase chain reaction; serology; surface proteins; ticks; vaccination; vaccines
Abstract:: ... Lyme disease in dogs can be effectively prevented by vaccination against antigens expressed by the spirochete Borrelia burgdorferi during transmission by the tick vector Ixodes sp. Lyme vaccine efficacy has traditionally been based on indicators of infection following wild-caught tick challenge whereas most other types of vaccine are required to demonstrate protection from clinical signs of diseas ...
DOI:: 10.1016/j.vetimm.2016.08.014
: https://doi.org/10.1016/j.vetimm.2016.08.014

80. Development of an autologous canine cancer vaccine system for resectable malignant tumors in dogs

Author:: J.R. Yannelli; R. Wouda; T.J. Masterson; M.G. Avdiushko; D.A. Cohen
Source:: Veterinary immunology and immunopathology 2016 v.182 pp. 95-100
ISSN:: 0165-2427
Subject:: Western blotting; adjuvants; antigens; biopsy; blood sampling; cell death; cell suspension culture; dogs; endotoxins; immune response; immunoglobulin G; immunotherapy; neoplasm cells; neoplasms; oligodeoxyribonucleotides; patients; ultraviolet radiation; vaccines; veterinary clinics
Abstract:: ... While conventional therapies exist for canine cancer, immunotherapies need to be further explored and applied to the canine setting. We have developed an autologous cancer vaccine (K9-ACV), which is available for all dogs with resectable disease. K9-ACV was evaluated for safety and immunogenicity for a variety of cancer types in a cohort of companion dogs under veterinary care. The autologous vacc ...
DOI:: 10.1016/j.vetimm.2016.10.011
: https://doi.org/10.1016/j.vetimm.2016.10.011

81. Development of recombinant BgP12 based enzyme linked immunosorbent assays for serodiagnosis of Babesia gibsoni infection in dogs

Author:: Mrityunjay Mandal; Partha S. Banerjee; Saroj Kumar; Rajat Garg; Hira Ram; Opinder K. Raina
Source:: Veterinary immunology and immunopathology 2016 v.169 pp. 27-33
ISSN:: 0165-2427
Subject:: Babesia canis subsp. vogeli; Babesia gibsoni; Dirofilaria immitis; Ehrlichia canis; Escherichia coli; Hepatozoon canis; Western blotting; blood serum; cross reaction; dog diseases; dogs; enzyme-linked immunosorbent assay; epidemiological studies; erythrocytes; genes; open reading frames; polymerase chain reaction; rabbits; serodiagnosis
Abstract:: ... Indirect ELISA and dot-ELISA using recombinant BgP12 (rBgP12) were developed for the diagnosis of Babesia gibsoni infected dogs. The complete open reading frame of BgP12 gene (378bp) was cloned in pET-32a(+) expression vector and expressed in Escherichia coli as a soluble thioredoxin (Trx) fusion protein. The purified rBgP12 was used for production of anti-rBgP12 rabbit serum, which recognized a n ...
DOI:: 10.1016/j.vetimm.2015.10.013
: https://doi.org/10.1016/j.vetimm.2015.10.013

82. Identification of histamine receptors in the canine gastrointestinal tract

Author:: Alyssa Sullivant; Andrew Mackin; Todd Pharr; Jim Cooley; Robert Wills; Todd Archer
Source:: Veterinary immunology and immunopathology 2016 v.182 pp. 29-36
ISSN:: 0165-2427
Subject:: Western blotting; adults; antibodies; biopsy; colon; digestive system diseases; dogs; euthanasia; ganglia; gastric mucosa; histamine; humans; ileum; immunohistochemistry; jejunum; mast cells; receptors; smooth muscle; staining
Abstract:: ... The important role of histamine in chronic gastrointestinal diseases has been increasingly recognized over the last two decades in human medicine. Histamine is released following mast cell activation and exerts its action through binding to four different histamine receptors (H1, H2, H3, and H4). Histamine receptors are dispersed throughout the body, and each different receptor mediates a unique r ...
DOI:: 10.1016/j.vetimm.2016.09.010
: https://doi.org/10.1016/j.vetimm.2016.09.010

83. Identification of linear B-cell epitopes on goose parvovirus non-structural protein

Author:: Tian-fei Yu; Bo Ma; Jun-wei Wang
Source:: Veterinary immunology and immunopathology 2016 v.179 pp. 85-88
ISSN:: 0165-2427
Subject:: Anser anser; Anseriform dependoparvovirus 1; B-lymphocytes; Cairina moschata; Escherichia coli; Western blotting; ducklings; epitopes; farming systems; financial economics; goslings; humoral immunity; immunoassays; peptides; viral nonstructural proteins
Abstract:: ... Goose parvovirus (GPV) infection can cause a highly contagious and lethal disease in goslings and muscovy ducklings which is widespread in all major goose (Anser anser) and Muscovy duck (Cairina moschata) farming countries, leading to a huge economic loss. Humoral immune responses play a major role in GPV immune protection during GPV infection. However, it is still unknown for the localization and ...
DOI:: 10.1016/j.vetimm.2016.08.009
: https://doi.org/10.1016/j.vetimm.2016.08.009

84. Identification of three novel B-cell epitopes of VMH protein from Vibrio mimicus by screening a phage display peptide library

Author:: Ning Xiao; Ji Cao; Hao Zhou; Shu-Quan Ding; Ling-Yan Kong; Jin-Nian Li
Source:: Veterinary immunology and immunopathology 2016 v.182 pp. 22-28
ISSN:: 0165-2427
Subject:: B-lymphocytes; Vibrio mimicus; Western blotting; antibodies; ascites; bacteriophages; clones; consensus sequence; enzyme-linked immunosorbent assay; epitopes; fish; fish diseases; peptide libraries; rabbits; screening; sequence analysis; synthetic peptides; vaccines
Abstract:: ... Vibrio mimicus is the causative agent of ascites disease in fish. The heat-labile hemolytic toxin designated VMH is an immunoprotective antigen of V. mimicus. However, its epitopes have not been well characterized. Here, a commercially available phage displayed 12-mer peptide library was used to screen epitopes of VMH protein using polyclonal rabbit anti-rVMH protein antibodies, and then five posi ...
DOI:: 10.1016/j.vetimm.2016.09.005
: https://doi.org/10.1016/j.vetimm.2016.09.005

85. Screening scFv antibodies against infectious bursal disease virus by co-expression of antigen and antibody in the bacteria display system

Author:: Xiaochen Guo; Hongxue Cao; Yunxin Wang; Yang Liu; Yanmin Chen; Nan Wang; Shan Jiang; Shengqi Zhang; Qiang Wu; Tianhe Li; Yingjie Zhang; Bing Zhou; Jiechao Yin; Deshan Li; Guiping Ren
Source:: Veterinary immunology and immunopathology 2016 v.180 pp. 45-52
ISSN:: 0165-2427
Subject:: Infectious bursal disease virus; Western blotting; antibody detection; antigens; bacteria; baiting; chickens; clones; enzyme-linked immunosorbent assay; flow cytometry; fluorescence; genes; neutralization; polyclonal antibodies; screening
Abstract:: ... We have previously reported an antigen and antibody co-expression (AAC) technology to demonstrate the interaction between a known antigen and antibody. To validate the co-expression system for screening antibody libraries, a single chain fragment variable(scFv)antibody library was constructed from chickens immunized with the VP2 protein of infectious bursal disease virus (IBDV). The genes of both ...
DOI:: 10.1016/j.vetimm.2016.09.004
: https://doi.org/10.1016/j.vetimm.2016.09.004

86. 17β-Estradiol and progesterone decrease MDP induced NOD2 expression in bovine mammary epithelial cells

Author:: Dan-dan Xu; Gang Wang; Xian-jing He; Jian-fa Wang; Bin Yang; Zhi-peng Sun; Dong-bo Sun; Qian-yu He; Xu Zhang; Rui Wu
Source:: Veterinary immunology and immunopathology 2017 v.188 pp. 59-64
ISSN:: 0165-2427
Subject:: Western blotting; animal tissues; chemical elements; dairy cows; epithelial cells; estradiol; humans; innate immunity; interleukin-6; interleukin-8; intracellular signaling peptides and proteins; laboratory animals; mammary glands; mastitis; messenger RNA; oligomerization; perinatal period; progesterone; protein kinases; protein synthesis; quantitative polymerase chain reaction; receptors; reverse transcriptase polymerase chain reaction; transcription (genetics); tumor necrosis factor-alpha
Abstract:: ... During the periparturient period, many neuroendocrine changes develop in cows. Periparturient hormone fluxes may adversely affect mammary gland immunity and mastitis susceptibility. 17β-Estradiol (E2) and progesterone (P4) have been reported to function on immune regulation, and their concentration fluctuates dramatically during the perinatal period. Nucleotide-binding oligomerization domain (NOD) ...
DOI:: 10.1016/j.vetimm.2017.04.010
: https://doi.org/10.1016/j.vetimm.2017.04.010

87. Comparative study of the vaccine potential of six outer membrane proteins of Edwardsiella tarda and the immune responses of flounder (Paralichthys olivaceus) after vaccination

Author:: Fuguo Liu; Xiaoqian Tang; Xiuzhen Sheng; Jing Xing; Wenbin Zhan
Source:: Veterinary immunology and immunopathology 2017 v.185 pp. 38-47
ISSN:: 0165-2427
Subject:: Edwardsiella tarda; Paralichthys olivaceus; Toll-like receptor 2; Western blotting; antibodies; blood serum; flounder; gene expression regulation; genes; immune response; interferon-gamma; interleukin-1beta; lymphocytes; messenger RNA; outer membrane proteins; spleen; tumor necrosis factor-alpha; vaccination; vaccines
Abstract:: ... To obtain effective vaccine candidates for Edwardsiella tarda, six outer membrane proteins (OMPs) of E. tarda were selected and recombinantly expressed. Western blotting analysis showed that flounder anti-E. tarda serum could recognize rOmpV, rOmpK, rOmpX and rOmpI, but not rOmpH and rOmpR. Immunization of flounder with different rOMPs resulted in high levels of protection following the E. tarda c ...
DOI:: 10.1016/j.vetimm.2017.01.008
: https://doi.org/10.1016/j.vetimm.2017.01.008

88. Investigation of corneal autoantibodies in horses with immune mediated keratitis (IMMK)

Author:: B.K. Braus; I. Miller; S. Kummer; K.J.H. Kleinwort; S. Hirmer; S.M. Hauck; R.J. McMullen; M. Kerschbaumer; C.A. Deeg
Source:: Veterinary immunology and immunopathology 2017 v.187 pp. 48-54
ISSN:: 0165-2427
Subject:: Western blotting; autoantibodies; blood serum; cell adhesion; cell movement; cornea; etiology; extracellular matrix; fluorescent antibody technique; horses; humans; immune response; keratitis; mass spectrometry; pain; proteins; proteome; screening; two-dimensional gel electrophoresis
Abstract:: ... Immune mediated keratitis (IMMK) is primarily a non-ulcerative keratitis in horses causing intermittent ocular pain, eventually resulting in visual impairment. Affected horses typically respond to immunomodulatory treatment. However, the underlying cause of the disease remains enigmatic. The current study was undertaken to investigate the presence of autoantibodies in horses with immune mediated k ...
DOI:: 10.1016/j.vetimm.2017.04.002
: https://doi.org/10.1016/j.vetimm.2017.04.002

89. Quantification of equine immunoglobulin A in serum and secretions by a fluorescent bead-based assay

Author:: Christiane L. Schnabel; Susanna Babasyan; Heather Freer; Bettina Wagner
Source:: Veterinary immunology and immunopathology 2017 v.188 pp. 12-20
ISSN:: 0165-2427
Subject:: Icelandic (horse breed); Thoroughbred; Western blotting; adults; blood serum; colostrum; cross reaction; fluorescence; horses; immunoglobulin A; immunoglobulin G; monoclonal antibodies; mucosa; mucosal immunity; nose; saliva
Abstract:: ... Only few quantitative reports exist about the concentrations and induction of immunoglobulin A (IgA) in mucosal secretions of horses. Despite this, it is widely assumed that IgA is the predominant immunoglobulin on mucosal surfaces in the horse.Here, two new monoclonal antibodies (mAbs) against equine IgA, clones 84-1 and 161-1, were developed and characterized in detail. Both IgA mAbs specificall ...
DOI:: 10.1016/j.vetimm.2017.04.001
: https://doi.org/10.1016/j.vetimm.2017.04.001

90. Acidic polypeptides A1a, A3 and A4 of Gly m 6 (glycinin) are allergenic for piglets

Author:: Shugui Zheng; Guixin Qin; Jing Chen; Fei Zhang
Source:: Veterinary immunology and immunopathology 2018 v.202 pp. 147-152
ISSN:: 0165-2427
Subject:: Western blotting; allergenicity; allergens; antibodies; binding capacity; enzyme-linked immunosorbent assay; glycinin; histamine; humans; hypersensitivity; immunoglobulin E; immunoglobulin G; piglets; polypeptides; skin prick tests; soybean meal; soybeans
Abstract:: ... Gly m 6 (glycinin) is one of the major antigenic proteins in soybeans responsible for transient hypersensitivity to soybean meal in weaned piglets. The globulin is a hexamer consisting of subunits containing basic and acidic polypeptides. Multiple acidic polypeptides have long been demonstrated to be allergens for humans and play a key role in the overall allergenicity of Gly m 6. To date, knowled ...
DOI:: 10.1016/j.vetimm.2018.06.003
: https://doi.org/10.1016/j.vetimm.2018.06.003

91. Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6

Author:: Yoshinori Shimamoto; Junko Nio-Kobayashi; Hiroshi Watarai; Masashi Nagano; Natsuko Saito; Eiki Takahashi; Hidetoshi Higuchi; Atsushi Kobayashi; Takashi Kimura; Hiroshi Kitamura
Source:: Veterinary immunology and immunopathology 2018 v.198 pp. 6-13
ISSN:: 0165-2427
Subject:: Western blotting; cattle; enzyme-linked immunosorbent assay; hematologic tests; immunohistochemistry; leukocytes; lymph nodes; macrophages; mastitis; monoclonal antibodies; prognosis; spleen
Abstract:: ... The scavenger receptor CD163 is widely used as a cell signature for alternatively active “M2” macrophages in mammals. In this study, we generated two monoclonal antibodies, ABM-1A9 and ABM-2D6, against the extracellular region of bovine CD163. Conventional Western blotting using the antibodies yielded immunoreactive bands of bovine CD163 at 130 and 150 kDa in non-reduced and reduced spleen lysates ...
DOI:: 10.1016/j.vetimm.2018.02.004
: https://doi.org/10.1016/j.vetimm.2018.02.004

92. Pharmacokinetics of human recombinant C1-esterase inhibitor and development of anti-drug antibodies in healthy dogs

Author:: Cheryl Wong; Daniela Hernandez Muguiro; Sidonie Lavergne; Erica Behling-Kelly; Robert Goggs
Source:: Veterinary immunology and immunopathology 2018 v.203 pp. 66-72
ISSN:: 0165-2427
Subject:: Beagle; Western blotting; adverse effects; antibodies; antibody formation; antigens; blood serum; clinical trials; complement; disease course; dogs; drugs; enzyme inhibitors; enzyme-linked immunosorbent assay; esterases; hemolysis; hemolytic anemia; humans; models; pharmacokinetics
Abstract:: ... Complement-mediated intravascular hemolysis occurs in canine immune-mediated hemolytic anemia (IMHA). Complement inhibitors such as recombinant C1 esterase inhibitor (rC1-INH) might prevent this process and alter the disease course. This study aimed to characterize the pharmacokinetics of a single 500 IU IV dose of rC1-INH in 8 healthy beagle dogs, evaluate the dogs for any adverse effects of drug ...
DOI:: 10.1016/j.vetimm.2018.08.006
: https://doi.org/10.1016/j.vetimm.2018.08.006

93. Use of ELISA and Western blot for serological detection of antibodies to E-S antigens of Trichinella spiralis muscle larvae in sera of swine experimentally infected with Trichinella spiralis

Author:: Michał Gondek; Justyna Bień; Zygmunt Nowakowski
Source:: Veterinary immunology and immunopathology 2018 v.203 pp. 13-20
ISSN:: 0165-2427
Subject:: Large White; Oesophagostomum; Trichinella spiralis; Western blotting; antibodies; antigens; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin G; molecular weight; muscle larvae; muscles; seroconversion; swine; trichinosis
Abstract:: ... The aim of the present study was to evaluate the detection of trichinellosis using ELISA, and to determine the degree of variation of IgG antibodies against excretory-secretory (E-S) antigens of T. spiralis muscle larvae. Ten young Polish Large White native breed pigs were experimentally inoculated with a low dose of 300 invasive Trichinella spiralis (T. spiralis) muscle larvae (ML). Pig sera were ...
DOI:: 10.1016/j.vetimm.2018.07.010
: https://doi.org/10.1016/j.vetimm.2018.07.010

94. Development and validation of an ELISA for the quantification of bovine ITIH4 in serum and milk

Author:: Lourdes Soler; Natalia García; Marta Andrés; Ramón Armengol; Fermín Lampreave; María A. Alava; Matilde Piñeiro
Source:: Veterinary immunology and immunopathology 2019 pp. -
ISSN:: 0165-2427
Subject:: Western blotting; acute phase proteins; blood serum; bovine mastitis; enzyme-linked immunosorbent assay; heifers; milk; monoclonal antibodies; polyclonal antibodies; summer; swine; trypsin inhibitors
Abstract:: ... Inter alpha trypsin inhibitor heavy chain 4 (ITIH4) is a serum protein belonging to the Inter alpha trypsin inhibitor (ITI) family, which was previously characterized by our group as a new APP in cattle. This protein was firstly described in pigs where is known to be a major acute phase protein, also denominated Pig-MAP. Increases of ITIH4 of up to 12 times the pre-infection values were previously ...
DOI:: 10.1016/j.vetimm.2019.109922
: https://doi.org/10.1016/j.vetimm.2019.109922

95. Development of a monoclonal antibody against swine leukocyte antigen (SLA)-DR α chain and evaluation of SLA-DR expression in bone marrow-derived dendritic cells after PRRSV infection

Author:: Tianshu Zhai; Chunyan Wu; Nana Wang; Binjun Shi; Jie Li; Rui Chen; Jianhui Dong; Yiying Zhang; En-Min Zhou; Yuchen Nan
Source:: Veterinary immunology and immunopathology 2019 v.211 pp. 19-24
ISSN:: 0165-2427
Subject:: Porcine reproductive and respiratory syndrome virus; Western blotting; antigens; cell lines; dendritic cells; immune response; messenger RNA; monoclonal antibodies; mononuclear leukocytes; porcine reproductive and respiratory syndrome; pork industry; protein content; swine
Abstract:: ... Porcine reproductive and respiratory syndrome (PRRS) is one of the most common diseases in the global swine industry. PRRSV infection is highly restricted to cells of the monocyte-macrophage lineage. However, the lack of antibodies to swine monocyte-macrophage lineage markers significantly hampers PRRSV research. In this study, we have developed a monoclonal antibody against the swine leukocyte an ...
DOI:: 10.1016/j.vetimm.2019.04.001
: https://doi.org/10.1016/j.vetimm.2019.04.001

96. Evaluation of immunogenicity and protective efficacy of a CpG-adjuvanted DNA vaccine against Tembusu virus

Author:: Hao Chen; Min Yan; Yi Tang; Youxiang Diao
Source:: Veterinary immunology and immunopathology 2019 v.218 pp. 109953
ISSN:: 0165-2427
Subject:: Tembusu virus; Western blotting; adjuvants; anorexia; blood serum; egg production; fever; fluorescent antibody technique; immunogenicity; immunopathology; interleukin-6; neutralization; oligodeoxyribonucleotides; pathogens; plasmids; recombinant vaccines; vaccination; waterfowl
Abstract:: ... Tembusu virus (TMUV) is a contagious pathogen of waterfowl including ducks and geese, which causes symptoms of high fever, loss of appetite and reduced egg production. The development of an effective vaccine is important for the prevention and control of the disease. We evaluated a DNA vaccine based on a recombinant pre-membrane (prM) and envelope (E) protein, using CpG oligodeoxynucleotide (ODN) ...
DOI:: 10.1016/j.vetimm.2019.109953
: https://doi.org/10.1016/j.vetimm.2019.109953

97. Development and characterization of novel mouse monoclonal antibodies against chicken chemokine CC motif ligand 4

Author:: Mingmin Lu; Woo H. Kim; Hyun S. Lillehoj; Charles Li
Source:: Veterinary immunology and immunopathology 2020 v.227 pp. 110091
ISSN:: 0165-2427
Subject:: Western blotting; antibody formation; antibody specificity; antigen detection; chemokine CCL4; chemotaxis; chickens; enzyme-linked immunosorbent assay; immunocytochemistry; mice; monoclonal antibodies; mononuclear leukocytes; neutralizing antibodies; protein synthesis
Abstract:: ... Chemokine (C-C motif) ligand (CCL) 4 is a CC chemokine subfamily member defined by the sequential positioning of conserved cysteine residues. Upon the binding of G-protein-coupled receptors on the cell surface, CCL4 mediates a diverse set of biological processes including chemotaxis, tumorigenesis, homeostasis and thymopoiesis. Although the physiological roles of mammalian CCL4s were elucidated >2 ...
Handle:: 10113/7015430
DOI:: 10.1016/j.vetimm.2020.110091
: https://doi.org/10.1016/j.vetimm.2020.110091

98. Preconditioning of canine adipose tissue-derived mesenchymal stem cells with deferoxamine potentiates anti-inflammatory effects by directing/reprogramming M2 macrophage polarization

Author:: Su-Min Park; Qiang Li; Min-Ok Ryu; Aryung Nam; Ju-Hyun An; Ji-In Yang; Sang-Min Kim; Woo-Jin Song; Hwa-Young Youn
Source:: Veterinary immunology and immunopathology 2020 v.219 pp. 109973
ISSN:: 0165-2427
Subject:: Western blotting; anti-inflammatory activity; coculture; deferoxamine; dogs; gene expression regulation; genes; hypoxia; macrophages; mesenchymal stromal cells; prostaglandins; quantitative polymerase chain reaction; secretion; transcription (genetics)
Abstract:: ... Preconditioning with hypoxia or hypoxia-mimetic agents has been tried with mesenchymal stem cells (MSCs) to improve the secretion of anti-inflammatory factors. These preconditioning procedures upregulate hypoxia inducible factor (HIF) 1-alpha leading to the transcription of HIF-dependent tissue protective and anti-inflammatory genes. Due to the limited number of studies exploring the activity of d ...
DOI:: 10.1016/j.vetimm.2019.109973
: https://doi.org/10.1016/j.vetimm.2019.109973

99. Genome editing of a hybridoma cell line via the CRISPR/Cas9 system: A new approach for constitutive high-level expression of heterologous proteins in eukaryotic system

Author:: Nicoletta Schibeci Natoli Scialli; Barbara Colitti; Luigi Bertolotti; Giulia Pezzoni; Eugenio Martignani; Maverick Melega; Emiliana Brocchi; Sergio Rosati
Source:: Veterinary immunology and immunopathology 2021 v.238 pp. 110286
ISSN:: 0165-2427
Subject:: Bovine alphaherpesvirus 1; CRISPR-Cas systems; DNA; RNA; Western blotting; culture media; diagnostic techniques; fluorometry; gene expression; genes; glycoproteins; green fluorescent protein; hybridomas; immunoglobulins; immunopathology; infectious diseases; mammals; protein synthesis; secretion
Abstract:: ... The power of the CRISPR/Cas9 system has revolutionized genome editing in many fields of biology. These applications have expanded exponentially over recent years, including those regarding protein expression technologies. The CRISPR/Cas9 system avoids random integration of the gene of interest and due to this characteristic can be exploited to obtain a stable cell line for the high-yield expressio ...
DOI:: 10.1016/j.vetimm.2021.110286
: https://doi.org/10.1016/j.vetimm.2021.110286

100. Evaluation of PD-1 and PD-L1 expression in canine urothelial carcinoma cell lines

Author:: Christopher J. Pinard; Samuel E. Hocker; Andrew C. Poon; Jordon M. Inkol; Arata Matsuyama; R. Darren Wood; Geoffrey A. Wood; J. Paul Woods; Anthony J. Mutsaers
Source:: Veterinary immunology and immunopathology 2022 v.243 pp. 110367
ISSN:: 0165-2427
Subject:: Western blotting; carcinoma; cell lines; dogs; flow cytometry; fluorescence microscopy; gene expression; humans; immunopathology; immunotherapy; neoplasm cells; reverse transcriptase polymerase chain reaction
Abstract:: ... Urothelial carcinoma (UC) is the most common urinary tumor in dogs and despite combinational therapies, only modest improvements in survival have been achieved in recent years. Given the utility of monoclonal antibodies against PD-1 and PD-L1 in human UC, we evaluated the protein and mRNA expression in three established canine urothelial carcinoma cell lines. Flow cytometry and western blot analys ...
DOI:: 10.1016/j.vetimm.2021.110367
: https://doi.org/10.1016/j.vetimm.2021.110367

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1. Characterisation of antisera to recombinant IgA of the common brushtail possum (Trichosurus vulpecula)

2. Cloning, expression, purification, and biological activity of five feline type I interferons

3. Development and characterization of monoclonal antibodies to chicken interleukin-15

4. Identification of monoclonal antibodies that cross-react with cytokines from different animal species

5. Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86)

6. Mannheimia haemolytica serotype 1 and Pasteurella trehalosi serotype 10 culture supernatants contain fibrinogen-binding proteins

7. Role of bovine chemokines produced by dendritic cells in respiratory syncytial virus-induced T cell proliferation

8. Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

9. Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE

10. Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs

11. Cloning of porcine scFv antibodies by phage display and expression in Escherichia coli

12. Development of a new monoclonal antibody to ovine chimeric IgE and its detection of systemic and local IgE antibody responses to the intestinal nematode Trichostrongylus colubriformis

13. Cloning of a gene fragment encoding bovine complement component C3d with expression and characterization of derived fusion proteins

14. Toll-like receptor 3 (TLR3): A new marker of canine monocytes-derived dendritic cells (cMo-DC)

15. Bovine lineage-specific gamma delta (γδ) T cell GD3.5 antibody cross-reacts with cell surface antigens on ovine and caprine lymphocytes

16. Cross-reactivity of mAbs to human CD antigens with cells from cattle [Erratum: 2008 Mar. 15, v. 122, issue 1-2, p. 188-189.]

17. Idiotype expression of IgG1 and IgG2 in dogs naturally infected with Leishmania infantum

18. Serum amyloid A isoforms in serum and synovial fluid from spontaneously diseased dogs with joint diseases or other conditions

19. Humoral response to Mycobacterium avium subsp. avium in naturally infected ring-neck doves (Streptopelia risoria)

20. Lack of detection of circulating skin-specific IgE autoantibodies in dogs with moderate or severe atopic dermatitis

21. Bovine immunoglobulin E

22. Characterization of the Apa antigen from M. avium subsp. paratuberculosis: A conserved Mycobacterium antigen that elicits a strong humoral response in cattle

23. Growth-inhibitory effects of four tyrosine kinase inhibitors on neoplastic feline mast cells exhibiting a Kit exon 8 ITD mutation

24. Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas

25. Molecular characterization of chicken prion proteins by C-terminal-specific monoclonal antibodies

26. Secretory expression of porcine interferon-gamma in baculovirus using HBM signal peptide and its inhibition activity on the replication of porcine reproductive and respiratory syndrome virus

27. A new method to discriminate immunogen-specific heavy-chain homodimer from heterotetramer immunoglobulin G directly in immunized dromedary whole plasma proteins: Western ligand blotting

28. Characterization of monoclonal antibodies against goat interleukin-18 and their application in the measurement of goat interleukin-18 in LPS-stimulated peripheral blood mononuclear cells by sandwich ELISA

29. Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays

30. Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off

31. TIR8 receptor expression in bovine tissues

32. Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis

33. Characterization of antibodies specific for canine TLR4, 5 and 9 by ELISA, Western blotting and immunohistochemistry

34. Cloning and antibody recognition analysis of the canine 5-lipoxygenase gene

35. Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen–Friedenreich antigen-specific Amaranthus caudatus lectin

36. Development of a multiplex assay for the detection of antibodies to Borrelia burgdorferi in horses and its validation using Bayesian and conventional statistical methods

37. Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells

38. Molecular cloning, in vitro expression and bioactivity of rabbit transforming growth factor-beta receptor type II (rTGF-βRII)

39. Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4

40. Molecular structure, phylogenetic analysis, tissue distribution, and function characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in sheep (Ovis aries)

41. PKC isoenzymes in equine platelets and stimulus induced activation

42. Selective cloning, characterization, and production of the Culicoides nubeculosus salivary gland allergen repertoire associated with equine insect bite hypersensitivity

43. A fluorescent bead-based multiplex assay for the simultaneous detection of antibodies to B. burgdorferi outer surface proteins in canine serum

44. Adenovirus-based oral vaccine for rabbit hemorrhagic disease

45. Binding affinity of anti-xylitol antibodies to canine hepatic vessels

46. Culicoides obsoletus extract relevant for diagnostics of insect bite hypersensitivity in horses

47. Development and characterization of a monoclonal antibody against porcine CD205

48. Development of ELISA test for determination of the level of antibodies against Rhodococcus equi in equine serum and colostrum

49. Generation of equine TSLP-specific antibodies and their use for detection of TSLP produced by equine keratinocytes and leukocytes

50. Immunoprecipitation of equine CD molecules using anti-human MABs previously analyzed by flow cytometry and immunohistochemistry

51. Localization of linear B-cell epitopes on goose parvovirus structural protein

52. Monoclonal antibodies recognizing mucus immunoglobulin and surface immunoglobulin-positive cells of flounder (Paralichthys olivaceus)

53. Monoclonal antibodies to equine CD23 identify the low-affinity receptor for IgE on subpopulations of IgM⁺ and IgG1⁺ B-cells in horses

54. Novel link between inflammation and impaired glucose transport during equine insulin resistance

55. Proteomic alteration of Marc-145 cells and PAMs after infection by porcine reproductive and respiratory syndrome virus

56. Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination

57. Characterization of the circulating serum amyloid A in bottlenose dolphins

58. Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)

59. Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines

60. Immunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer's disease in pigs

61. Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells

62. Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes

63. Recombinant adenovirus expressing F and H fusion proteins of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in goats

64. Anti-lymphoproliferative activity of alpha-2-macroglobulin in the plasma of hibernating 13-lined ground squirrels and woodchucks

65. Characterization of arginase expression by equine neutrophils

66. Egg yolk IgY against RHDV capsid protein VP60 promotes rabbit defense against RHDV infection

67. Immunoproteomics of Brucella abortus RB51 as candidate antigens in serological diagnosis of brucellosis

68. A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

69. TLR4 is constitutively expressed in chick thymic epithelial cells

70. Toll-like receptor and pro-inflammatory cytokine expression during prolonged hyperinsulinaemia in horses: Implications for laminitis