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Veterinary immunology and immunopathology
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- Author:
- Christopher J. Pinard; Samuel E. Hocker; Andrew C. Poon; Jordon M. Inkol; Arata Matsuyama; R. Darren Wood; Geoffrey A. Wood; J. Paul Woods; Anthony J. Mutsaers
- Source:
- Veterinary immunology and immunopathology 2022 v.243 pp. 110367
- ISSN:
- 0165-2427
- Subject:
- Western blotting; carcinoma; cell lines; dogs; flow cytometry; fluorescence microscopy; gene expression; humans; immunopathology; immunotherapy; neoplasm cells; reverse transcriptase polymerase chain reaction
- Abstract:
- ... Urothelial carcinoma (UC) is the most common urinary tumor in dogs and despite combinational therapies, only modest improvements in survival have been achieved in recent years. Given the utility of monoclonal antibodies against PD-1 and PD-L1 in human UC, we evaluated the protein and mRNA expression in three established canine urothelial carcinoma cell lines. Flow cytometry and western blot analys ...
- DOI:
- 10.1016/j.vetimm.2021.110367
- https://doi.org/10.1016/j.vetimm.2021.110367
- Author:
- Nicoletta Schibeci Natoli Scialli; Barbara Colitti; Luigi Bertolotti; Giulia Pezzoni; Eugenio Martignani; Maverick Melega; Emiliana Brocchi; Sergio Rosati
- Source:
- Veterinary immunology and immunopathology 2021 v.238 pp. 110286
- ISSN:
- 0165-2427
- Subject:
- Bovine alphaherpesvirus 1; CRISPR-Cas systems; DNA; RNA; Western blotting; culture media; diagnostic techniques; fluorometry; gene expression; genes; glycoproteins; green fluorescent protein; hybridomas; immunoglobulins; immunopathology; infectious diseases; mammals; protein synthesis; secretion
- Abstract:
- ... The power of the CRISPR/Cas9 system has revolutionized genome editing in many fields of biology. These applications have expanded exponentially over recent years, including those regarding protein expression technologies. The CRISPR/Cas9 system avoids random integration of the gene of interest and due to this characteristic can be exploited to obtain a stable cell line for the high-yield expressio ...
- DOI:
- 10.1016/j.vetimm.2021.110286
- https://doi.org/10.1016/j.vetimm.2021.110286
- Author:
- Mingmin Lu; Woo H. Kim; Hyun S. Lillehoj; Charles Li
- Source:
- Veterinary immunology and immunopathology 2020 v.227 pp. 110091
- ISSN:
- 0165-2427
- Subject:
- Western blotting; antibody formation; antibody specificity; antigen detection; chemokine CCL4; chemotaxis; chickens; enzyme-linked immunosorbent assay; immunocytochemistry; mice; monoclonal antibodies; mononuclear leukocytes; neutralizing antibodies; protein synthesis
- Abstract:
- ... Chemokine (C-C motif) ligand (CCL) 4 is a CC chemokine subfamily member defined by the sequential positioning of conserved cysteine residues. Upon the binding of G-protein-coupled receptors on the cell surface, CCL4 mediates a diverse set of biological processes including chemotaxis, tumorigenesis, homeostasis and thymopoiesis. Although the physiological roles of mammalian CCL4s were elucidated >2 ...
- Handle:
- 10113/7015430
- DOI:
- 10.1016/j.vetimm.2020.110091
- https://doi.org/10.1016/j.vetimm.2020.110091
- Author:
- Su-Min Park; Qiang Li; Min-Ok Ryu; Aryung Nam; Ju-Hyun An; Ji-In Yang; Sang-Min Kim; Woo-Jin Song; Hwa-Young Youn
- Source:
- Veterinary immunology and immunopathology 2020 v.219 pp. 109973
- ISSN:
- 0165-2427
- Subject:
- Western blotting; anti-inflammatory activity; coculture; deferoxamine; dogs; gene expression regulation; genes; hypoxia; macrophages; mesenchymal stromal cells; prostaglandins; quantitative polymerase chain reaction; secretion; transcription (genetics)
- Abstract:
- ... Preconditioning with hypoxia or hypoxia-mimetic agents has been tried with mesenchymal stem cells (MSCs) to improve the secretion of anti-inflammatory factors. These preconditioning procedures upregulate hypoxia inducible factor (HIF) 1-alpha leading to the transcription of HIF-dependent tissue protective and anti-inflammatory genes. Due to the limited number of studies exploring the activity of d ...
- DOI:
- 10.1016/j.vetimm.2019.109973
- https://doi.org/10.1016/j.vetimm.2019.109973
- Author:
- Lourdes Soler; Natalia García; Marta Andrés; Ramón Armengol; Fermín Lampreave; María A. Alava; Matilde Piñeiro
- Source:
- Veterinary immunology and immunopathology 2019 pp. -
- ISSN:
- 0165-2427
- Subject:
- Western blotting; acute phase proteins; blood serum; bovine mastitis; enzyme-linked immunosorbent assay; heifers; milk; monoclonal antibodies; polyclonal antibodies; summer; swine; trypsin inhibitors
- Abstract:
- ... Inter alpha trypsin inhibitor heavy chain 4 (ITIH4) is a serum protein belonging to the Inter alpha trypsin inhibitor (ITI) family, which was previously characterized by our group as a new APP in cattle. This protein was firstly described in pigs where is known to be a major acute phase protein, also denominated Pig-MAP. Increases of ITIH4 of up to 12 times the pre-infection values were previously ...
- DOI:
- 10.1016/j.vetimm.2019.109922
- https://doi.org/10.1016/j.vetimm.2019.109922
- Author:
- Tianshu Zhai; Chunyan Wu; Nana Wang; Binjun Shi; Jie Li; Rui Chen; Jianhui Dong; Yiying Zhang; En-Min Zhou; Yuchen Nan
- Source:
- Veterinary immunology and immunopathology 2019 v.211 pp. 19-24
- ISSN:
- 0165-2427
- Subject:
- Porcine reproductive and respiratory syndrome virus; Western blotting; antigens; cell lines; dendritic cells; immune response; messenger RNA; monoclonal antibodies; mononuclear leukocytes; porcine reproductive and respiratory syndrome; pork industry; protein content; swine
- Abstract:
- ... Porcine reproductive and respiratory syndrome (PRRS) is one of the most common diseases in the global swine industry. PRRSV infection is highly restricted to cells of the monocyte-macrophage lineage. However, the lack of antibodies to swine monocyte-macrophage lineage markers significantly hampers PRRSV research. In this study, we have developed a monoclonal antibody against the swine leukocyte an ...
- DOI:
- 10.1016/j.vetimm.2019.04.001
- https://doi.org/10.1016/j.vetimm.2019.04.001
- Author:
- Hao Chen; Min Yan; Yi Tang; Youxiang Diao
- Source:
- Veterinary immunology and immunopathology 2019 v.218 pp. 109953
- ISSN:
- 0165-2427
- Subject:
- Tembusu virus; Western blotting; adjuvants; anorexia; blood serum; egg production; fever; fluorescent antibody technique; immunogenicity; immunopathology; interleukin-6; neutralization; oligodeoxyribonucleotides; pathogens; plasmids; recombinant vaccines; vaccination; waterfowl
- Abstract:
- ... Tembusu virus (TMUV) is a contagious pathogen of waterfowl including ducks and geese, which causes symptoms of high fever, loss of appetite and reduced egg production. The development of an effective vaccine is important for the prevention and control of the disease. We evaluated a DNA vaccine based on a recombinant pre-membrane (prM) and envelope (E) protein, using CpG oligodeoxynucleotide (ODN) ...
- DOI:
- 10.1016/j.vetimm.2019.109953
- https://doi.org/10.1016/j.vetimm.2019.109953
- Author:
- Shugui Zheng; Guixin Qin; Jing Chen; Fei Zhang
- Source:
- Veterinary immunology and immunopathology 2018 v.202 pp. 147-152
- ISSN:
- 0165-2427
- Subject:
- Western blotting; allergenicity; allergens; antibodies; binding capacity; enzyme-linked immunosorbent assay; glycinin; histamine; humans; hypersensitivity; immunoglobulin E; immunoglobulin G; piglets; polypeptides; skin prick tests; soybean meal; soybeans
- Abstract:
- ... Gly m 6 (glycinin) is one of the major antigenic proteins in soybeans responsible for transient hypersensitivity to soybean meal in weaned piglets. The globulin is a hexamer consisting of subunits containing basic and acidic polypeptides. Multiple acidic polypeptides have long been demonstrated to be allergens for humans and play a key role in the overall allergenicity of Gly m 6. To date, knowled ...
- DOI:
- 10.1016/j.vetimm.2018.06.003
- https://doi.org/10.1016/j.vetimm.2018.06.003
- Author:
- Yoshinori Shimamoto; Junko Nio-Kobayashi; Hiroshi Watarai; Masashi Nagano; Natsuko Saito; Eiki Takahashi; Hidetoshi Higuchi; Atsushi Kobayashi; Takashi Kimura; Hiroshi Kitamura
- Source:
- Veterinary immunology and immunopathology 2018 v.198 pp. 6-13
- ISSN:
- 0165-2427
- Subject:
- Western blotting; cattle; enzyme-linked immunosorbent assay; hematologic tests; immunohistochemistry; leukocytes; lymph nodes; macrophages; mastitis; monoclonal antibodies; prognosis; spleen
- Abstract:
- ... The scavenger receptor CD163 is widely used as a cell signature for alternatively active “M2” macrophages in mammals. In this study, we generated two monoclonal antibodies, ABM-1A9 and ABM-2D6, against the extracellular region of bovine CD163. Conventional Western blotting using the antibodies yielded immunoreactive bands of bovine CD163 at 130 and 150 kDa in non-reduced and reduced spleen lysates ...
- DOI:
- 10.1016/j.vetimm.2018.02.004
- https://doi.org/10.1016/j.vetimm.2018.02.004
- Author:
- Racheal Aye; Yenehiwot Berhanu Weldearegay; Harrison Osundwa Lutta; Francis Chuma; Andreas Pich; Joerg Jores; Jochen Meens; Jan Naessens
- Source:
- Veterinary immunology and immunopathology 2018 v.204 pp. 11-18
- ISSN:
- 0165-2427
- Subject:
- Mycoplasma mycoides; Western blotting; active sites; acyltransferases; agglutination; antigens; blood serum; cattle; epithelial cells; lungs; monoclonal antibodies; polysaccharides; proteins; rabbits; vaccines
- Abstract:
- ... Mycoplasma mycoides subspecies mycoides (Mmm) adhesion is tissue and host specific. Inhibition of adhesion will prevent Mmm from binding to lung cells and hence prevent colonization and disease. The aim of this study was to develop a panel of Mmm monoclonal antibodies against Mmm and use these antibodies to investigate their inhibitory effect on the adherence of Mmm to bovine lung epithelial cells ...
- DOI:
- 10.1016/j.vetimm.2018.09.002
- PubMed:
- 30596376
- PubMed Central:
- PMC6215757
- https://doi.org/10.1016/j.vetimm.2018.09.002
- Author:
- Atul A. Chaudhari; Woo H. Kim; Hyun S. Lillehoj
- Source:
- Veterinary immunology and immunopathology 2018 v.205 pp. 72-82
- ISSN:
- 0165-2427
- Subject:
- Western blotting; arginase; cell lines; chemokines; chickens; enzyme activity; enzyme-linked immunosorbent assay; flow cytometry; gene expression; genes; humans; immunocytochemistry; inducible nitric oxide synthase; interleukin-13; interleukin-4; ligands; lipopolysaccharides; macrophages; mannose; mice; monoclonal antibodies; monocytes; neutralization; nitric oxide; phenotype
- Abstract:
- ... In mammals, alternatively activated macrophages (AAMs) are well-recognized and are produced by stimulation with Th2 cytokines such as interleukin 4 (IL-4) and IL-13. On their mammalian counterparts, AAMs in chickens has neither been reported nor the functionality of chicken IL-4 (chIL-4) has been studied till date. Therefore, present study developed mouse monoclonal antibodies (mAbs) against chIL- ...
- DOI:
- 10.1016/j.vetimm.2018.10.011
- CHORUS:
- 10.1016/j.vetimm.2018.10.011
- https://doi.org/10.1016/j.vetimm.2018.10.011
- Author:
- Cheryl Wong; Daniela Hernandez Muguiro; Sidonie Lavergne; Erica Behling-Kelly; Robert Goggs
- Source:
- Veterinary immunology and immunopathology 2018 v.203 pp. 66-72
- ISSN:
- 0165-2427
- Subject:
- Beagle; Western blotting; adverse effects; antibodies; antibody formation; antigens; blood serum; clinical trials; complement; disease course; dogs; drugs; enzyme inhibitors; enzyme-linked immunosorbent assay; esterases; hemolysis; hemolytic anemia; humans; models; pharmacokinetics
- Abstract:
- ... Complement-mediated intravascular hemolysis occurs in canine immune-mediated hemolytic anemia (IMHA). Complement inhibitors such as recombinant C1 esterase inhibitor (rC1-INH) might prevent this process and alter the disease course. This study aimed to characterize the pharmacokinetics of a single 500 IU IV dose of rC1-INH in 8 healthy beagle dogs, evaluate the dogs for any adverse effects of drug ...
- DOI:
- 10.1016/j.vetimm.2018.08.006
- https://doi.org/10.1016/j.vetimm.2018.08.006
- Author:
- Michał Gondek; Justyna Bień; Zygmunt Nowakowski
- Source:
- Veterinary immunology and immunopathology 2018 v.203 pp. 13-20
- ISSN:
- 0165-2427
- Subject:
- Large White; Oesophagostomum; Trichinella spiralis; Western blotting; antibodies; antigens; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin G; molecular weight; muscle larvae; muscles; seroconversion; swine; trichinosis
- Abstract:
- ... The aim of the present study was to evaluate the detection of trichinellosis using ELISA, and to determine the degree of variation of IgG antibodies against excretory-secretory (E-S) antigens of T. spiralis muscle larvae. Ten young Polish Large White native breed pigs were experimentally inoculated with a low dose of 300 invasive Trichinella spiralis (T. spiralis) muscle larvae (ML). Pig sera were ...
- DOI:
- 10.1016/j.vetimm.2018.07.010
- https://doi.org/10.1016/j.vetimm.2018.07.010
- Author:
- Dan-dan Xu; Gang Wang; Xian-jing He; Jian-fa Wang; Bin Yang; Zhi-peng Sun; Dong-bo Sun; Qian-yu He; Xu Zhang; Rui Wu
- Source:
- Veterinary immunology and immunopathology 2017 v.188 pp. 59-64
- ISSN:
- 0165-2427
- Subject:
- Western blotting; animal tissues; chemical elements; dairy cows; epithelial cells; estradiol; humans; innate immunity; interleukin-6; interleukin-8; intracellular signaling peptides and proteins; laboratory animals; mammary glands; mastitis; messenger RNA; oligomerization; perinatal period; progesterone; protein kinases; protein synthesis; quantitative polymerase chain reaction; receptors; reverse transcriptase polymerase chain reaction; transcription (genetics); tumor necrosis factor-alpha
- Abstract:
- ... During the periparturient period, many neuroendocrine changes develop in cows. Periparturient hormone fluxes may adversely affect mammary gland immunity and mastitis susceptibility. 17β-Estradiol (E2) and progesterone (P4) have been reported to function on immune regulation, and their concentration fluctuates dramatically during the perinatal period. Nucleotide-binding oligomerization domain (NOD) ...
- DOI:
- 10.1016/j.vetimm.2017.04.010
- https://doi.org/10.1016/j.vetimm.2017.04.010
- Author:
- Fuguo Liu; Xiaoqian Tang; Xiuzhen Sheng; Jing Xing; Wenbin Zhan
- Source:
- Veterinary immunology and immunopathology 2017 v.185 pp. 38-47
- ISSN:
- 0165-2427
- Subject:
- Edwardsiella tarda; Paralichthys olivaceus; Toll-like receptor 2; Western blotting; antibodies; blood serum; flounder; gene expression regulation; genes; immune response; interferon-gamma; interleukin-1beta; lymphocytes; messenger RNA; outer membrane proteins; spleen; tumor necrosis factor-alpha; vaccination; vaccines
- Abstract:
- ... To obtain effective vaccine candidates for Edwardsiella tarda, six outer membrane proteins (OMPs) of E. tarda were selected and recombinantly expressed. Western blotting analysis showed that flounder anti-E. tarda serum could recognize rOmpV, rOmpK, rOmpX and rOmpI, but not rOmpH and rOmpR. Immunization of flounder with different rOMPs resulted in high levels of protection following the E. tarda c ...
- DOI:
- 10.1016/j.vetimm.2017.01.008
- https://doi.org/10.1016/j.vetimm.2017.01.008
- Author:
- Emily M. Martin; Samuel L. Jones
- Source:
- Veterinary immunology and immunopathology 2017 v.192 pp. 33-40
- ISSN:
- 0165-2427
- Subject:
- Western blotting; adults; adverse effects; enzyme-linked immunosorbent assay; gastrointestinal system; granulocyte-macrophage colony-stimulating factor; horses; indomethacin; inflammation; leukocytes; lipopolysaccharides; messenger RNA; mixed breeds; models; nonsteroidal anti-inflammatory agents; patients; prostacyclin; prostaglandin synthase; quantitative polymerase chain reaction; therapeutics
- Abstract:
- ... Inhibition of prostaglandin E2 (PGE2) production effectively limits inflammation in horses, however nonspecific prostaglandin blockade via cyclooxygenase (COX) inhibition elicits deleterious gastrointestinal side effects in equine patients. Thus, more selective PGE2 targeting therapeutics are needed to treat inflammatory disease in horses. One potential target is microsomal prostaglandin E-synthas ...
- DOI:
- 10.1016/j.vetimm.2017.09.008
- PubMed:
- 29042013
- PubMed Central:
- PMC6038704
- https://doi.org/10.1016/j.vetimm.2017.09.008
- Author:
- B.K. Braus; I. Miller; S. Kummer; K.J.H. Kleinwort; S. Hirmer; S.M. Hauck; R.J. McMullen; M. Kerschbaumer; C.A. Deeg
- Source:
- Veterinary immunology and immunopathology 2017 v.187 pp. 48-54
- ISSN:
- 0165-2427
- Subject:
- Western blotting; autoantibodies; blood serum; cell adhesion; cell movement; cornea; etiology; extracellular matrix; fluorescent antibody technique; horses; humans; immune response; keratitis; mass spectrometry; pain; proteins; proteome; screening; two-dimensional gel electrophoresis
- Abstract:
- ... Immune mediated keratitis (IMMK) is primarily a non-ulcerative keratitis in horses causing intermittent ocular pain, eventually resulting in visual impairment. Affected horses typically respond to immunomodulatory treatment. However, the underlying cause of the disease remains enigmatic. The current study was undertaken to investigate the presence of autoantibodies in horses with immune mediated k ...
- DOI:
- 10.1016/j.vetimm.2017.04.002
- https://doi.org/10.1016/j.vetimm.2017.04.002
18. Quantification of equine immunoglobulin A in serum and secretions by a fluorescent bead-based assay
- Author:
- Christiane L. Schnabel; Susanna Babasyan; Heather Freer; Bettina Wagner
- Source:
- Veterinary immunology and immunopathology 2017 v.188 pp. 12-20
- ISSN:
- 0165-2427
- Subject:
- Icelandic (horse breed); Thoroughbred; Western blotting; adults; blood serum; colostrum; cross reaction; fluorescence; horses; immunoglobulin A; immunoglobulin G; monoclonal antibodies; mucosa; mucosal immunity; nose; saliva
- Abstract:
- ... Only few quantitative reports exist about the concentrations and induction of immunoglobulin A (IgA) in mucosal secretions of horses. Despite this, it is widely assumed that IgA is the predominant immunoglobulin on mucosal surfaces in the horse.Here, two new monoclonal antibodies (mAbs) against equine IgA, clones 84-1 and 161-1, were developed and characterized in detail. Both IgA mAbs specificall ...
- DOI:
- 10.1016/j.vetimm.2017.04.001
- https://doi.org/10.1016/j.vetimm.2017.04.001
- Author:
- Heather Freer; Julia M. Hillegas; Christine Wimer; Cynthia Baldwin; Joanna LaBresh; Bettina Wagner
- Source:
- Veterinary immunology and immunopathology 2017 v.191 pp. 30-35
- ISSN:
- 0165-2427
- Subject:
- CD4-positive T-lymphocytes; Phytolaccaceae; Western blotting; blood serum; cell differentiation; cell growth; colostrum; concanavalin A; detection limit; enzyme-linked immunosorbent assay; flow cytometry; fluorescence; horses; interleukin-2; lipopolysaccharides; monoclonal antibodies; secretion; staining; yeasts
- Abstract:
- ... Interleukin-2 (IL-2) is a T cell growth factor and major modulator of T helper (Th) cell differentiation. Here, we have developed and characterized a monoclonal antibody to equine IL-2 (anti-IL-2 mAb, clone 158-1). The IL-2 mAb detected rIL-2 by ELISA, intracellular staining and flow cytometry analysis and Western blotting. The IL-2 mAb was also paired with a polyclonal IL-2 detection antibody in ...
- DOI:
- 10.1016/j.vetimm.2017.07.011
- CHORUS:
- 10.1016/j.vetimm.2017.07.011
- https://doi.org/10.1016/j.vetimm.2017.07.011
- Author:
- Deborah A. Grosenbaugh; Daniel R. Rissi; Paula M. Krimer
- Source:
- Veterinary immunology and immunopathology 2016 v.180 pp. 29-33
- ISSN:
- 0165-2427
- Subject:
- Beagle; Borrelia burgdorferi; Ixodes scapularis; Lyme disease; Western blotting; antigens; biopsy; disease vectors; dog diseases; dogs; histopathology; lameness; models; polymerase chain reaction; serology; surface proteins; ticks; vaccination; vaccines
- Abstract:
- ... Lyme disease in dogs can be effectively prevented by vaccination against antigens expressed by the spirochete Borrelia burgdorferi during transmission by the tick vector Ixodes sp. Lyme vaccine efficacy has traditionally been based on indicators of infection following wild-caught tick challenge whereas most other types of vaccine are required to demonstrate protection from clinical signs of diseas ...
- DOI:
- 10.1016/j.vetimm.2016.08.014
- https://doi.org/10.1016/j.vetimm.2016.08.014
21. Development of an autologous canine cancer vaccine system for resectable malignant tumors in dogs
- Author:
- J.R. Yannelli; R. Wouda; T.J. Masterson; M.G. Avdiushko; D.A. Cohen
- Source:
- Veterinary immunology and immunopathology 2016 v.182 pp. 95-100
- ISSN:
- 0165-2427
- Subject:
- Western blotting; adjuvants; antigens; biopsy; blood sampling; cell death; cell suspension culture; dogs; endotoxins; immune response; immunoglobulin G; immunotherapy; neoplasm cells; neoplasms; oligodeoxyribonucleotides; patients; ultraviolet radiation; vaccines; veterinary clinics
- Abstract:
- ... While conventional therapies exist for canine cancer, immunotherapies need to be further explored and applied to the canine setting. We have developed an autologous cancer vaccine (K9-ACV), which is available for all dogs with resectable disease. K9-ACV was evaluated for safety and immunogenicity for a variety of cancer types in a cohort of companion dogs under veterinary care. The autologous vacc ...
- DOI:
- 10.1016/j.vetimm.2016.10.011
- https://doi.org/10.1016/j.vetimm.2016.10.011
- Author:
- Mrityunjay Mandal; Partha S. Banerjee; Saroj Kumar; Rajat Garg; Hira Ram; Opinder K. Raina
- Source:
- Veterinary immunology and immunopathology 2016 v.169 pp. 27-33
- ISSN:
- 0165-2427
- Subject:
- Babesia canis subsp. vogeli; Babesia gibsoni; Dirofilaria immitis; Ehrlichia canis; Escherichia coli; Hepatozoon canis; Western blotting; blood serum; cross reaction; dog diseases; dogs; enzyme-linked immunosorbent assay; epidemiological studies; erythrocytes; genes; open reading frames; polymerase chain reaction; rabbits; serodiagnosis
- Abstract:
- ... Indirect ELISA and dot-ELISA using recombinant BgP12 (rBgP12) were developed for the diagnosis of Babesia gibsoni infected dogs. The complete open reading frame of BgP12 gene (378bp) was cloned in pET-32a(+) expression vector and expressed in Escherichia coli as a soluble thioredoxin (Trx) fusion protein. The purified rBgP12 was used for production of anti-rBgP12 rabbit serum, which recognized a n ...
- DOI:
- 10.1016/j.vetimm.2015.10.013
- https://doi.org/10.1016/j.vetimm.2015.10.013
- Author:
- Alyssa Sullivant; Andrew Mackin; Todd Pharr; Jim Cooley; Robert Wills; Todd Archer
- Source:
- Veterinary immunology and immunopathology 2016 v.182 pp. 29-36
- ISSN:
- 0165-2427
- Subject:
- Western blotting; adults; antibodies; biopsy; colon; digestive system diseases; dogs; euthanasia; ganglia; gastric mucosa; histamine; humans; ileum; immunohistochemistry; jejunum; mast cells; receptors; smooth muscle; staining
- Abstract:
- ... The important role of histamine in chronic gastrointestinal diseases has been increasingly recognized over the last two decades in human medicine. Histamine is released following mast cell activation and exerts its action through binding to four different histamine receptors (H1, H2, H3, and H4). Histamine receptors are dispersed throughout the body, and each different receptor mediates a unique r ...
- DOI:
- 10.1016/j.vetimm.2016.09.010
- https://doi.org/10.1016/j.vetimm.2016.09.010
- Author:
- Tian-fei Yu; Bo Ma; Jun-wei Wang
- Source:
- Veterinary immunology and immunopathology 2016 v.179 pp. 85-88
- ISSN:
- 0165-2427
- Subject:
- Anser anser; Anseriform dependoparvovirus 1; B-lymphocytes; Cairina moschata; Escherichia coli; Western blotting; ducklings; epitopes; farming systems; financial economics; goslings; humoral immunity; immunoassays; peptides; viral nonstructural proteins
- Abstract:
- ... Goose parvovirus (GPV) infection can cause a highly contagious and lethal disease in goslings and muscovy ducklings which is widespread in all major goose (Anser anser) and Muscovy duck (Cairina moschata) farming countries, leading to a huge economic loss. Humoral immune responses play a major role in GPV immune protection during GPV infection. However, it is still unknown for the localization and ...
- DOI:
- 10.1016/j.vetimm.2016.08.009
- https://doi.org/10.1016/j.vetimm.2016.08.009
- Author:
- Ning Xiao; Ji Cao; Hao Zhou; Shu-Quan Ding; Ling-Yan Kong; Jin-Nian Li
- Source:
- Veterinary immunology and immunopathology 2016 v.182 pp. 22-28
- ISSN:
- 0165-2427
- Subject:
- B-lymphocytes; Vibrio mimicus; Western blotting; antibodies; ascites; bacteriophages; clones; consensus sequence; enzyme-linked immunosorbent assay; epitopes; fish; fish diseases; peptide libraries; rabbits; screening; sequence analysis; synthetic peptides; vaccines
- Abstract:
- ... Vibrio mimicus is the causative agent of ascites disease in fish. The heat-labile hemolytic toxin designated VMH is an immunoprotective antigen of V. mimicus. However, its epitopes have not been well characterized. Here, a commercially available phage displayed 12-mer peptide library was used to screen epitopes of VMH protein using polyclonal rabbit anti-rVMH protein antibodies, and then five posi ...
- DOI:
- 10.1016/j.vetimm.2016.09.005
- https://doi.org/10.1016/j.vetimm.2016.09.005
- Author:
- Xiaochen Guo; Hongxue Cao; Yunxin Wang; Yang Liu; Yanmin Chen; Nan Wang; Shan Jiang; Shengqi Zhang; Qiang Wu; Tianhe Li; Yingjie Zhang; Bing Zhou; Jiechao Yin; Deshan Li; Guiping Ren
- Source:
- Veterinary immunology and immunopathology 2016 v.180 pp. 45-52
- ISSN:
- 0165-2427
- Subject:
- Infectious bursal disease virus; Western blotting; antibody detection; antigens; bacteria; baiting; chickens; clones; enzyme-linked immunosorbent assay; flow cytometry; fluorescence; genes; neutralization; polyclonal antibodies; screening
- Abstract:
- ... We have previously reported an antigen and antibody co-expression (AAC) technology to demonstrate the interaction between a known antigen and antibody. To validate the co-expression system for screening antibody libraries, a single chain fragment variable(scFv)antibody library was constructed from chickens immunized with the VP2 protein of infectious bursal disease virus (IBDV). The genes of both ...
- DOI:
- 10.1016/j.vetimm.2016.09.004
- https://doi.org/10.1016/j.vetimm.2016.09.004
- Author:
- Yun Sun; Yong-hua Hu
- Source:
- Veterinary immunology and immunopathology 2015 v.167 no.3-4 pp. 96-103
- ISSN:
- 0165-2427
- Subject:
- immunoglobulin M; Western blotting; antigen-presenting cells; cell membranes; vaccination; Streptococcus iniae; macrophage activation; antibodies; lymphocytes; recombinant fusion proteins; gene expression; transactivators; gene expression regulation; subunit vaccines; Drosophila; interleukin-8; Paralichthys olivaceus; blood serum; Human immunodeficiency virus 1; antigens; flounder; homeodomain proteins; transcription (genetics); tumor necrosis factor-alpha; kidneys; bacterial vaccines; interleukin-1beta; survival rate; protein synthesis; fluorescence microscopy
- Abstract:
- ... The efficiency of antigen capture, processing, and presentation by antigen-presenting cells is the key to induce an effective immune response. Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargoes and have an attractive potential for vaccine delivery. In this study, the Drosophila Antennapedia homeoprotein (Antp) and the human immunodefici ...
- DOI:
- 10.1016/j.vetimm.2015.07.008
- https://doi.org/10.1016/j.vetimm.2015.07.008
- Author:
- Xiaokai Song; Lixin Xu; Ruofeng Yan; Xinmei Huang; Xiangrui Li
- Source:
- Veterinary immunology and immunopathology 2015 v.166 no.3-4 pp. 79-87
- ISSN:
- 0165-2427
- Subject:
- Eimeria tenella; T-lymphocytes; Western blotting; adjuvants; animal experimentation; body weight changes; chickens; coccidiosis; coccidiostats; epitopes; genes; immune response; interferon-gamma; interleukin-2; laboratory animals; merozoites; oocysts; parasites; recombinant vaccines; reverse transcriptase polymerase chain reaction; survival rate; translation (genetics); vaccine development
- Abstract:
- ... The search for effective vaccines against chicken coccidiosis remains a challenge because of the complex organisms with multiple life cycle stages of Eimeria. Combination of T-cell epitopes from different stages of Eimeria life cycle could be an optimal strategy to overcome the antigen complexity of the parasite. In this study, 4 fragments with concentrated T-cell epitopes from the sporozoite anti ...
- DOI:
- 10.1016/j.vetimm.2015.05.005
- https://doi.org/10.1016/j.vetimm.2015.05.005
- Author:
- Xuelian Zhang; Jianwei Shao; Chunqiu Cao; Bo Ma; Junwei Wang
- Source:
- Veterinary immunology and immunopathology 2015 v.168 no.3-4 pp. 135-139
- ISSN:
- 0165-2427
- Subject:
- T-lymphocytes; Western blotting; antigens; geese; monoclonal antibodies; recombinant proteins
- Abstract:
- ... CD3 is one of the most important cell surface markers of T lymphocytes, and has an important function in signal transmission during antigen recognition. In this study, monoclonal antibodies against goose CD3ɛ extracellular domain were developed and characterized for the first time. The purified recombinant goose CD3ɛ extracellular domain protein and pcDNA3.1-GoCD3ɛex were used as immunogens to dev ...
- DOI:
- 10.1016/j.vetimm.2015.10.012
- https://doi.org/10.1016/j.vetimm.2015.10.012
- Author:
- Nan-Nan Liu; Zeng-Shan Liu; Shi-Ying Lu; Pan Hu; Yan-Song Li; Xiao-Li Feng; Shou-Yin Zhang; Nan Wang; Qing-Feng Meng; Yong-Jie Yang; Feng Tang; Yun-Ming Xu; Wen-Hui Zhang; Xing Guo; Xiao-Feng Chen; Yu Zhou; Hong-Lin Ren
- Source:
- Veterinary immunology and immunopathology 2015 v.164 no.3-4 pp. 208-219
- ISSN:
- 0165-2427
- Subject:
- phospholipase A2; reactive oxygen species; Western blotting; Brucella melitensis; heart; open reading frames; biomarkers; recombinant proteins; rapid amplification of cDNA ends; leukocytes; monoclonal antibodies; amino acids; intestines; spleen; homeostasis; stomach; molecular weight; lymph nodes; gene expression regulation; tissues; virulence; messenger RNA; complementary DNA; peroxiredoxin; muscles; immune response; kidneys; brucellosis; protein synthesis; liver; sheep; vaccines; glutathione peroxidase
- Abstract:
- ... Peroxiredoxin 6 (Prdx6), an important antioxidant enzyme that can eliminate reactive oxygen species (ROS) to maintain homeostasis, is a bifunctional protein that possesses the activities of both glutathione peroxidase and phospholipase A2. In this study, a novel full-length Prdx6 cDNA (OaPrdx6) was cloned from Sheep (Ovis aries) using rapid amplification of cDNA ends (RACE). The full-length cDNA o ...
- DOI:
- 10.1016/j.vetimm.2015.01.006
- https://doi.org/10.1016/j.vetimm.2015.01.006
- Author:
- Gerardo Pavel Espino-Solis; Veronica Quintero-Hernandez; Alejandro Olvera-Rodriguez; Juana Calderon-Amador; Martha Pedraza-Escalona; Alexei Licea-Navarro; Leopoldo Flores-Romo; Lourival Domingos Possani
- Source:
- Veterinary immunology and immunopathology 2015 v.164 no.3-4 pp. 220-226
- ISSN:
- 0165-2427
- Subject:
- Escherichia coli; Western blotting; dendritic cells; enzyme-linked immunosorbent assay; epitopes; horses; immunohistochemistry; integrins; lymph nodes; mice; monoclonal antibodies; mononuclear leukocytes; vaccines
- Abstract:
- ... The αX I-domain of the horse integrin CD11c was successfully expressed in Escherichia coli, purified, biochemically characterized and used as immunogen to generate murine monoclonal antibodies against horse CD11c, which are not yet commercially available. One monoclonal antibody mAb-1C4 against the αX I-domain, is an IgG2a able to interact with the recombinant I-domain, showing an EC50=2.4ng accor ...
- DOI:
- 10.1016/j.vetimm.2015.02.002
- https://doi.org/10.1016/j.vetimm.2015.02.002
- Author:
- Yingqi Zhu; Chuanfeng Li; Zhuangli Bi; Zongyan Chen; Chunchun Meng; Gui-Jun Wang; Chan Ding; Guangqing Liu
- Source:
- Veterinary immunology and immunopathology 2015 v.165 no.1-2 pp. 88-92
- ISSN:
- 0165-2427
- Subject:
- Reoviridae; Semliki Forest virus; Western blotting; ducklings; fluorescent antibody technique; genes; immune response; interferon-gamma; interleukin-4; neutralization; neutralizing antibodies; recombinant vaccines; replicon; vaccine development
- Abstract:
- ... A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV). The sigma C gene of NDRV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. After transfected into BHK-21 cells, the antigenicity of the sigma C protein was confirmed using an indirect immunofluorescence and Western blot assay. ...
- DOI:
- 10.1016/j.vetimm.2015.03.001
- https://doi.org/10.1016/j.vetimm.2015.03.001
- Author:
- Yadu Balachandran; Steven Knaus; Sarah Caldwell; Baljit Singh
- Source:
- Veterinary immunology and immunopathology 2015 v.168 no.3-4 pp. 184-192
- ISSN:
- 0165-2427
- Subject:
- Escherichia coli; Toll-like receptor 10; Western blotting; antibodies; cattle; chickens; dogs; endothelium; human diseases; innate immunity; lipopolysaccharides; lungs; molecular weight; muscles; neutrophils; plasma membrane; rats; swine
- Abstract:
- ... Toll-like receptors (TLRs) are conserved immune receptors that play critical roles in innate immunity and are known as “gate keepers” of immune system. TLR10 is identified as type 1 plasma membrane protein but the identity of its ligand remains unclear. Till date, no data are available on tissue and cell specific expression of TLR10 in normal and inflamed lungs of domestic animal species, and rat, ...
- DOI:
- 10.1016/j.vetimm.2015.09.007
- https://doi.org/10.1016/j.vetimm.2015.09.007
- Author:
- Fabrizio Ceciliani; Laura Soler; Guido Grilli; Andreia T. Marques; Chiara Giudice; Cristina Lecchi
- Source:
- Veterinary immunology and immunopathology 2015 v.168 no.1-2 pp. 35-39
- ISSN:
- 0165-2427
- Subject:
- Western blotting; acute phase proteins; adipocytes; adipose tissue; amyloid; blood serum; blood vessels; cattle; endothelial cells; gene expression; gene expression regulation; hepatocytes; immunohistochemistry; liver; messenger RNA; parenchyma (animal tissue); polyclonal antibodies; polymerase chain reaction; staining; withers
- Abstract:
- ... In this article the localization of the acute phase protein Serum Amyloid A (SAA) in different depots of bovine adipose tissue (AT) and liver is reported. Quantitative (Real Time) PCR was paired to immunohistochemistry after the production of a specific polyclonal antibody. SAA's mRNA was found in all analyzed AT depots included in the present study, the AT located in the withers being the major s ...
- DOI:
- 10.1016/j.vetimm.2015.08.004
- https://doi.org/10.1016/j.vetimm.2015.08.004
- Author:
- Donna G. Sieckmann; Howard Jaffe; Susanne Golech; DeCheng Cai; John M. Hallenbeck; Richard M. McCarron
- Source:
- Veterinary immunology and immunopathology 2014 v.161 no.1-2 pp. 1-11
- ISSN:
- 0165-2427
- Subject:
- Marmota monax; Western blotting; cell culture; cold; concanavalin A; gel chromatography; hibernation; high performance liquid chromatography; humans; mass spectrometry; mice; molecular weight; spleen; squirrels
- Abstract:
- ... Plasma from hibernating (HIB) woodchucks (Marmota monax) or 13-lined ground squirrels (Ictidomys tridecemlineatus) suppressed 3H-thymidine uptake in mouse spleen cell cultures stimulated with Concanavalin A (ConA); plasma from non-hibernating animals were only slightly inhibitory. Maximum inhibition occurred when HIB plasma was added to the cultures prior to ConA. After HPLC size exclusion chromat ...
- DOI:
- 10.1016/j.vetimm.2014.05.006
- https://doi.org/10.1016/j.vetimm.2014.05.006
- Author:
- Anouk Lavoie-Lamoureux; James G. Martin; Jean-Pierre Lavoie
- Source:
- Veterinary immunology and immunopathology 2014 v.157 no.3-4 pp. 206-213
- ISSN:
- 0165-2427
- Subject:
- Western blotting; arginase; enzyme activity; gene expression; horses; interleukin-4; messenger RNA; metabolism; neutrophils; ornithine decarboxylase; ornithine-oxo-acid transaminase; polymerase chain reaction
- Abstract:
- ... Neutrophils are the predominant cells recruited in the airways of horses suffering from heaves. These cells have been shown to express arginase in some species. The metabolism of l-arginine is thought to be involved in chronic inflammation, and airway obstruction and remodeling. The aim of this study was to assess the expression, regulation, activity, and functional role of arginase isoforms in eq ...
- DOI:
- 10.1016/j.vetimm.2013.12.007
- https://doi.org/10.1016/j.vetimm.2013.12.007
- Author:
- Sung Hyen Lee; Hyun S. Lillehoj; Misun Jeong; Emilio Del Cacho; Wongi Min; Yvonne B. Sullivan; Laura Kakach; Joanna W. LaBresh; Haeng Ran Kim
- Source:
- Veterinary immunology and immunopathology 2014 v.159 no.1-2 pp. 103-109
- ISSN:
- 0165-2427
- Subject:
- Escherichia; T-lymphocytes; Western blotting; antigens; bursa of Fabricius; cecum; chickens; cytotoxicity; endothelial cells; enzyme-linked immunosorbent assay; lymphocyte proliferation; mice; monoclonal antibodies; necrosis; necrotic enteritis; nitric oxide; poultry production; spleen; tonsils; tumor necrosis factors
- Abstract:
- ... Tumor necrosis factor-like ligand 1A (TL1A) is a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. However, there have not been any mAbs which specifically detect chTL1A and define its biochemical and immunolog ...
- Handle:
- 10113/59001
- DOI:
- 10.1016/j.vetimm.2014.01.002
- https://doi.org/10.1016/j.vetimm.2014.01.002
- Author:
- Zai Xin Li; Wei Dong Hu; Bing Chao Li; Tian You Li; Xiao Yang Zhou; Zhi Zhang
- Source:
- Veterinary immunology and immunopathology 2014 v.157 no.1-2 pp. 97-104
- ISSN:
- 0165-2427
- Subject:
- Escherichia coli; Rabbit hemorrhagic disease virus; Western blotting; antigens; antiserum; capsid; chickens; chromatography; egg yolk; enzyme-linked immunosorbent assay; immune response; immunoglobulin Y; plasmids; rabbits; therapeutics; viruses
- Abstract:
- ... VP60 capsid protein is the major structural and immunogenicity protein of RHDV (Rabbit hemorrhagic disease virus, RHDV), and has been implicated as a main protein antigen in RHDV diagnosis and vaccine design. In this report, egg yolk antibody (IgY) against N-terminal of VP60 was evaluated and developed as a new strategy for RHDV therapy. Briefly, N-terminal of VP60 (∼250aa) fragment was cloned and ...
- DOI:
- 10.1016/j.vetimm.2013.10.002
- https://doi.org/10.1016/j.vetimm.2013.10.002
- Author:
- Ji-Yeon Kim; So-Ra Sung; Kichan Lee; Hyang-Keun Lee; Sung-Il Kang; Jin Ju Lee; Suk Chan Jung; Yong Ho Park; Moon Her
- Source:
- Veterinary immunology and immunopathology 2014 v.160 no.3-4 pp. 218-224
- ISSN:
- 0165-2427
- Subject:
- Brucella melitensis biovar Abortus; Escherichia coli; Gram-negative bacteria; Western blotting; Yersinia enterocolitica; aldehyde dehydrogenase; antibodies; antigens; antiserum; brucellosis; cattle; cell division; chaperonins; cross reaction; lipopolysaccharides; mutants; pH; serodiagnosis; superoxide dismutase; two-dimensional gel electrophoresis
- Abstract:
- ... The current brucellosis serodiagnostic assays are chiefly based on detecting anti-LPS (lipopolysaccharide) antibodies. However, cross-reaction with some gram-negative bacteria can occasionally induce due to similar O-polysaccharide (OPS) structure. Therefore, the aim of the present study was to identify new candidate antigens from Brucella abortus RB51, a mutant strain lacking the LPS portion, whi ...
- DOI:
- 10.1016/j.vetimm.2014.05.009
- https://doi.org/10.1016/j.vetimm.2014.05.009
- Author:
- Yanina P. Hecker; Verónica Cóceres; Silvina E. Wilkowsky; José M. Jaramillo Ortiz; Eleonora L. Morrell; Andrea E. Verna; Agustina Ganuza; Dora B. Cano; Lilian Lischinsky; Sergio O. Ángel; Patricia Zamorano; Anselmo C. Odeón; María R. Leunda; Carlos M. Campero; Bror Morein; Dadín P. Moore
- Source:
- Veterinary immunology and immunopathology 2014 v.162 no.3-4 pp. 142-153
- ISSN:
- 0165-2427
- Subject:
- Escherichia coli; Neospora caninum; Western blotting; antibodies; antigens; fetus; heifers; immune response; immunization; immunohistochemistry; interferon-gamma; intravenous injection; nickel; polymerase chain reaction; pregnancy; protein synthesis; recombinant proteins; transplacental transmission; vaccines
- Abstract:
- ... The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20 and rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of Neospora caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were ...
- DOI:
- 10.1016/j.vetimm.2014.11.001
- https://doi.org/10.1016/j.vetimm.2014.11.001
- Author:
- Hai-Bo Huang; Quan-Hang Xiang; Hui Wu; Abdur Rahman Ansari; Le Wen; Xiao-Hong Ge; Ji-Xiang Wang; Ke-Mei Peng; Hua-Zhen Liu
- Source:
- Veterinary immunology and immunopathology 2014 v.158 no.3-4 pp. 182-188
- ISSN:
- 0165-2427
- Subject:
- Toll-like receptor 4; Western blotting; chicks; epithelial cells; gene expression; gilts; immunohistochemistry; juveniles; messenger RNA; protein synthesis; quantitative polymerase chain reaction; thymus gland
- Abstract:
- ... Toll-like receptor 4 (TLR4) has been suggested to play a regulatory role in immune cell development; however, studies regarding the role of TLR4 in the development of the chick thymus are scarce. In this study, we investigated the distribution and expression pattern of TLR4 in normal chick thymi at different stages of development, in order to better understand the role of TLR4 in chick thymus deve ...
- DOI:
- 10.1016/j.vetimm.2014.01.005
- https://doi.org/10.1016/j.vetimm.2014.01.005
- Author:
- M.A. de Laat; C.K. Clement; C.M. McGowan; M.N. Sillence; C.C. Pollitt; V.A. Lacombe
- Source:
- Veterinary immunology and immunopathology 2014 v.157 no.1-2 pp. 78-86
- ISSN:
- 0165-2427
- Subject:
- Standardbred; Western blotting; glucose; hooves; horses; hyperinsulinemia; interleukin-6; laminitis; muscle protein; protein synthesis; skeletal muscle; tumor necrosis factor-alpha
- Abstract:
- ... Equine laminitis, a disease of the lamellar structure of the horse's hoof, can be incited by numerous factors that include inflammatory and metabolic aetiologies. However, the role of inflammation in hyperinsulinaemic laminitis has not been adequately defined. Toll-like receptor (TLR) activation results in up-regulation of inflammatory pathways and the release of pro-inflammatory cytokines, includ ...
- DOI:
- 10.1016/j.vetimm.2013.10.010
- https://doi.org/10.1016/j.vetimm.2013.10.010
- Author:
- Takao Segawa; Toru Otsuka; Takuya Itou; Miwa Suzuki; Nana Karatani; Takeo Sakai
- Source:
- Veterinary immunology and immunopathology 2013 v.152 no.3-4 pp. 218-224
- ISSN:
- 0165-2427
- Subject:
- Tursiops truncatus; Western blotting; amino acid sequences; amyloid; dolphins; inflammation; isoelectric focusing; isoelectric point; liver; messenger RNA; swine
- Abstract:
- ... Several isoforms of serum amyloid A (SAA) have been identified so far and because the plasma concentration of it increases dramatically, it is used as an indicator of inflammation in animals. In many terrestrial mammals, the circulating isoforms are SAA1 and SAA2, which are synthesized in the liver. Extra-hepatically synthesized SAA3, however, is a predominantly local SAA isoform with a characteri ...
- DOI:
- 10.1016/j.vetimm.2012.12.006
- PubMed:
- 23333194
- https://doi.org/10.1016/j.vetimm.2012.12.006
- Author:
- Victoria A. Valdenegro-Vega; Philip Crosbie; Benita Vincent; Kenneth D. Cain; Barbara F. Nowak
- Source:
- Veterinary immunology and immunopathology 2013 v.151 no.1-2 pp. 113-123
- ISSN:
- 0165-2427
- Subject:
- Salmo salar; Western blotting; antibodies; antibody formation; antigens; enzyme-linked immunosorbent assay; explants; fish; fluorescein; immunization; mucus
- Abstract:
- ... This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens – dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) – administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post ...
- DOI:
- 10.1016/j.vetimm.2012.10.010
- PubMed:
- 23206403
- https://doi.org/10.1016/j.vetimm.2012.10.010
45. Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines
- Author:
- C.L. Schnabel; S. Wagner; B. Wagner; M.C. Durán; S. Babasyan; I. Nolte; C. Pfarrer; K. Feige; H. Murua Escobar; J.-M.V. Cavalleri
- Source:
- Veterinary immunology and immunopathology 2013 v.156 no.1-2 pp. 1-19
- ISSN:
- 0165-2427
- Subject:
- Western blotting; cross reaction; flow cytometry; granulocyte-macrophage colony-stimulating factor; horses; immunohistochemistry; interleukin-12; interleukin-18; interleukin-6; interleukin-8; macrophages; messenger RNA; monoclonal antibodies; mononuclear leukocytes; swine
- Abstract:
- ... Research on equine cytokines is often performed by analyses of mRNA. For many equine cytokines an analysis on the actual protein level is limited by the availability of antibodies against the targeted cytokines. Generation of new antibodies is ongoing but time consuming. Thus, testing the reactivity of commercially available antibodies for cross-reactivity with equine cytokines is of particular in ...
- DOI:
- 10.1016/j.vetimm.2013.09.012
- PubMed:
- 24139393
- https://doi.org/10.1016/j.vetimm.2013.09.012
- Author:
- Sonia Martínez-Martínez; Rafael Frandoloso; Elías F. Rodríguez Ferri; Concha Gil; Carolina Hernández-Haro; Sheila Yubero; César B. Gutiérrez Martín
- Source:
- Veterinary immunology and immunopathology 2013 v.151 no.3-4 pp. 235-247
- ISSN:
- 0165-2427
- Subject:
- Haemophilus parasuis; Western blotting; antibodies; antigens; electrophoresis; gels; glyceraldehyde 3-phosphate; immunization; immunoglobulin A; immunoglobulin G; immunoglobulin M; peptidylprolyl isomerase; serotypes; sialidase; subunit vaccines; swine; swine diseases; transferrin
- Abstract:
- ... An immunoproteomic analysis of the protective response of subunit and commercial vaccines in colostrum-deprived pigs against Glässer's disease was carried out. A mixture of proteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasaki strain (serovar 5) was inoculated intramuscularly (PAPTM) and intratracheally (PAPTCp), along with a commercial bacterin. PAPT were separat ...
- DOI:
- 10.1016/j.vetimm.2012.11.014
- PubMed:
- 23266097
- https://doi.org/10.1016/j.vetimm.2012.11.014
- Author:
- Yunhe Fu; Bo Liu; Xiaosheng Feng; Zhicheng Liu; Dejie Liang; Fengyang Li; Depeng Li; Yongguo Cao; Shuang Feng; Xichen Zhang; Naisheng Zhang; Zhengtao Yang
- Source:
- Veterinary immunology and immunopathology 2013 v.151 no.1-2 pp. 20-27
- ISSN:
- 0165-2427
- Subject:
- Escherichia coli; Gram-negative bacteria; Western blotting; cattle; endometrium; epithelial cells; immunity; lipopolysaccharides; polymerase chain reaction; receptors; transcription factors
- Abstract:
- ... The endometrium is easily contaminated with bacteria and the endometrial epithelial cells (EECs) play an important role in defence against invading pathogens which recognized pathogen-associated molecular patterns (PAMPs) via pattern recognition receptors (PRRs). Toll-like receptor 4 (TLR4) can recognize lipopolysaccharide (LPS) from Gram-negative bacteria and initiates innate immune responses. In ...
- DOI:
- 10.1016/j.vetimm.2012.09.039
- PubMed:
- 23218938
- https://doi.org/10.1016/j.vetimm.2012.09.039
- Author:
- Shingo Maeda; Sadatoshi Maeda; Koichi Ohno; Noriyuki Kaji; Masatoshi Hori; Yasuhito Fujino; Hajime Tsujimoto
- Source:
- Veterinary immunology and immunopathology 2013 v.153 no.1-2 pp. 17-25
- ISSN:
- 0165-2427
- Subject:
- granulocyte-macrophage colony-stimulating factor; Western blotting; receptors; trypsin; gene expression; tumor necrosis factors; interleukin-8; messenger RNA; humans; dogs; proteolysis; keratinocytes; fluorescent antibody technique; fluorescent dyes; allergenicity; inflammation; allergens; atopic dermatitis; agonists
- Abstract:
- ... Although the molecular basis of the allergenicity remains to be fully elucidated, the ability of allergens to elicit allergic responses is at least partly attributed to their proteolytic activity. Protease-activated receptor-2 (PAR-2) is a G protein-coupled receptor that is activated by site-specific proteolysis by serine proteases and is known to mediate inflammatory processes in various tissues. ...
- DOI:
- 10.1016/j.vetimm.2013.01.018
- PubMed:
- 23465358
- https://doi.org/10.1016/j.vetimm.2013.01.018
- Author:
- Yong Wang; Guangqing Liu; Zongyan Chen; Chuanfeng Li; Lijun Shi; Wenchao Li; Huaxin Huang; Chunai Tao; Chaofei Cheng; Binrui Xu; Gang Li
- Source:
- Veterinary immunology and immunopathology 2013 v.154 no.1-2 pp. 1-7
- ISSN:
- 0165-2427
- Subject:
- Adenoviridae; Western blotting; acute course; antibodies; fluorescent antibody technique; glycoproteins; goats; hosts; humoral immunity; immune response; immunization; lymphocyte proliferation; neutralization; peste des petits ruminants; seroconversion; vaccines; viruses
- Abstract:
- ... Peste des petits ruminants (PPR) is an acute and contagious disease of some small ruminants caused by peste des petits ruminants virus (PPRV). Fusion (F) protein and hemagglutinin (H) protein are two glycoproteins of PPRV that might induce a protective immune response. In this study, three replication-defective recombinant adenoviruses were constructed and the immunogenicity was evaluated in goats ...
- DOI:
- 10.1016/j.vetimm.2013.05.002
- PubMed:
- 23707075
- https://doi.org/10.1016/j.vetimm.2013.05.002
- Author:
- Xinglong Wang; Li Qiu; Huafang Hao; Weidong Zhang; Xiangjing Fu; Hongling Zhang; Shengfang He; Shuxia Zhang; Enqi Du; Zengqi Yang
- Source:
- Veterinary immunology and immunopathology 2012 v.145 no.1-2 pp. 277-282
- ISSN:
- 0165-2427
- Subject:
- Rabbit hemorrhagic disease virus; Western blotting; antibodies; antigens; biosecurity; coat proteins; immune response; immunization; liver; protein synthesis; rabbit diseases; rabbits; vaccines
- Abstract:
- ... Vaccine antigens for rabbit hemorrhagic disease virus (RHDV) are currently derived from inactivated RHDV obtained from the livers of experimentally infected rabbits or from several recombinant immunogens. However, the application of these vaccine antigens has been restricted because of biosecurity and immunity characteristics. In the current study, a recombinant adenovirus expressing the RHDV caps ...
- DOI:
- 10.1016/j.vetimm.2011.11.014
- PubMed:
- 22154735
- https://doi.org/10.1016/j.vetimm.2011.11.014
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