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You searched for: Journal Veterinary immunology and immunopathology Remove constraint Journal: Veterinary immunology and immunopathology
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1. Acetoacetate is a trigger of NLRP3 inflammasome activation in bovine peripheral blood mononuclear cells

Author:
Yuri Onizawa; Taiga Katoh; Ryotaro Miura; Kunitoshi Konda; Tatsuo Noguchi; Hisataka Iwata; Takehito Kuwayama; Seizo Hamano; Koumei Shirasuna
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110370
ISSN:
0165-2427
Subject:
acetoacetic acid; blood plasma; caspase-1; estrous cycle; gene expression; immunopathology; inflammasomes; inflammation; ketone bodies; metabolomics; repeat breeder; reproductive disorders; secretion
Abstract:
... Repeat breeding, which is non-pregnancy following three or more breeding attempts, is a serious reproductive disorder in cattle. In the present study, metabolomic profiling was used to identify metabolites in the blood plasma of repeat breeder cows (RBCs) and non-RBCs. Metabolomic analysis showed that acetoacetate (AcAc), a ketone body, was detected in RBCs, but not in non-RBCs. In contrast, β-hyd ...
DOI:
10.1016/j.vetimm.2021.110370

2. Antibodies from healthy or paratuberculosis infected cows have different effects on Mycobacterium avium subspecies paratuberculosis invasion in a calf ileal loop model

Author:
Ana Jolly; Bárbara Fernández; Ana Stempler; Giselle Ingratta; Gabriela Postma; Juan Boviez; Daniel Lombardo; Silvia Hajos; Silvia L. Mundo
Source:
Veterinary immunology and immunopathology 2022 v.245 pp. 110381
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; calves; histology; histopathology; ileum; immunopathology; microbial load; models; paratuberculosis; protective effect; viability
Abstract:
... In this work, we used a calf ileal loop model to evaluate whether the preincubation of Mycobacterium avium subspecies paratuberculosis (MAP) with antibodies from healthy, MAP-positive or Lipoarabinomannan (LAM) immunized cows could affect the results of infection after 3.5 h. Bacterial load in tissue was assessed by Ziehl-Neelsen and by culture for each loop. MAP was detectable in all infected loo ...
DOI:
10.1016/j.vetimm.2022.110381

3. Antibodies that react with bovine T lymphocytes expressing the T cell receptor β chain subgroup BV20 inhibit antigen recognition

Author:
W. Ivan Morrison; Tara Sheldrake; Timothy Connelley
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110392
ISSN:
0165-2427
Subject:
antigens; cattle; cytotoxicity; gene duplication; genes; immunopathology
Abstract:
... In recent years, molecular studies have provided detailed information on the bovine T cell receptor (TCR) variable gene repertoire, both in resting T cells and during T cell responses. However, studies of the biological function of the receptor have been hampered by a lack of reagents that recognise the protein. Herein, we describe the characterisation of two antibodies (IL-A47 and IL-A98) that re ...
DOI:
10.1016/j.vetimm.2022.110392

4. Assessment of heterologous lumpy skin disease vaccine-induced immunity in pregnant cattle vaccinated at different times of gestation period and their influence on maternally derived antibodies

Author:
Ahmed Gaber; Sherin Rouby; Amera Elsaied; Ahmed El-Sherif
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110380
ISSN:
0165-2427
Subject:
cell-mediated immunity; gestation period; humoral immunity; immunopathology; longevity; lumpy skin disease; maternal immunity; parturition; pregnancy; sheep pox; vaccination; vaccines
Abstract:
... The present study aimed to evaluate the cell-mediated and the humoral immune response to Romanian sheep pox vaccine in pregnant cows (n = 12) vaccinated at different times of gestation period and the duration of maternal immunity in calves born to these cows. Evaluation of cellular immunity revealed an increase in lymphocytic proliferation that peaked at 10th day post vaccination (dpv) then gradua ...
DOI:
10.1016/j.vetimm.2021.110380

5. B cell memory responses induced by foot-and-mouth disease virus-like particles in BALB/c mice

Author:
Jia-huan Liu; Jun-juan Zhang; Wei-jian Han; Chuan Cui; Ming-zhu Li; Zhan-yun Tian; Ruo-man Bai; Li-min Li
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110458
ISSN:
0165-2427
Subject:
antibodies; blood serum; bone marrow; foot-and-mouth disease; immunization; immunoglobulin G; immunopathology; interleukin-6; memory; spleen; vaccine development
Abstract:
... A challenging but critical question is that new foot-and-mouth disease (FMD) vaccines should be to induce B cell memory to provide antibodies for long-term protection. The maintenance of B cell memory is dependent on long-lived plasma cells (LLPCs) and memory B cells. We developed a chimeric FMDV virus-like particles (FMDV-VLPs), fusing VP1-VP4 into HBcAg. In our study, we investigated if or how l ...
DOI:
10.1016/j.vetimm.2022.110458

6. CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification

Author:
Cintia Hiromi Okino; Wilson Malagó Junior; Cintia Righetti Marcondes; Rodrigo Giglioti; Hélio José Montassier; Henrique Nunes de Oliveira; Márcia Cristina de Sena Oliveira
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110462
ISSN:
0165-2427
Subject:
genes; genotyping; heterozygosity; homozygosity; hybrids; immunopathology; immunophenotyping; taurine; zebu
Abstract:
... Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozy ...
DOI:
10.1016/j.vetimm.2022.110462

7. Canine memory T-cell subsets in health and disease

Author:
Annie B. Bauman; Cambri E. Moeller; Aimee M. Soileau; Christine E. Goodermuth; Victoria R. Costa; Jessica C. Schaumburg; Cherie M. Pucheu-Haston; Nancy D. Welborn; Shannon D. Dehghanpir; Mark A. Mitchell; Sita S. Withers
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110401
ISSN:
0165-2427
Subject:
T-lymphocytes; antigens; atopic dermatitis; dogs; drug therapy; flow cytometry; immunopathology; immunotherapy; inflammation; lymph nodes; lymphoma; memory; prednisone
Abstract:
... A more complete understanding of canine T-lymphocyte immunity is necessary for improving diagnostic and therapeutic approaches to canine diseases, developing cell-based canine immunotherapeutics, and evaluating dogs as large mammal models for comparative immunology research. The aim of this study was to utilize CD45RA (indicating antigen inexperience) and CD62L (indicating lymph node homing capabi ...
DOI:
10.1016/j.vetimm.2022.110401

8. Changes in rabbit lymphocyte subpopulations and activation following long-term administration of meloxicam

Author:
Pavla Hamouzová; Šárka Stehlíková; Kristína Řeháková; Vladimír Jekl; Jan Chloupek; Jaroslav Doubek
Source:
Veterinary immunology and immunopathology 2022 v.253 pp. 110496
ISSN:
0165-2427
Subject:
B-lymphocytes; analgesics; flow cytometry; hematology; immunology; immunopathology; meloxicam; rabbits
Abstract:
... Meloxicam is a commonly used analgesic in rabbits. However, its possible impact on lymphocyte subpopulations remained unknown. The aim of the study was to investigate a possible effect of long-term administration of meloxicam on rabbit lymphocyte subpopulations. The study included 8 rabbits given meloxicam orally once daily (1 mg/kg BW) for 14 days and 8 rabbits as a control group. Peripheral bloo ...
DOI:
10.1016/j.vetimm.2022.110496

9. Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression

Author:
Sara Marzo; Mark Gray; Waywen Loh; Lindsey A. Waddell; C. Irene McGuinnes; Jayne C. Hope; Heather A. Mathie
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110363
ISSN:
0165-2427
Subject:
T-lymphocytes; adaptive immunity; cattle; dendritic cells; flow cytometry; immunopathology; lymph; models; phenotype
Abstract:
... The bovine afferent lymphatic cannulation model allows collection of large volumes of afferent lymph and provides an opportunity to study lymphatic cells trafficking from the periphery directly ex-vivo. The technique requires surgical intervention, but influence of the procedure or time post-surgery on cells trafficking in the lymph has not been well documented. Here, we measured the volume of lym ...
DOI:
10.1016/j.vetimm.2021.110363

10. Chitosan-based formulation of hemagglutinin antigens for oculo-nasal booster vaccination of chickens against influenza viruses

Author:
Violetta Sączyńska; Agnieszka Romanik-Chruścielewska; Katarzyna Florys-Jankowska; Violetta Cecuda-Adamczewska; Małgorzata Kęsik-Brodacka
Source:
Veterinary immunology and immunopathology 2022 v.247 pp. 110406
ISSN:
0165-2427
Subject:
Escherichia coli; aluminum hydroxide; avian influenza; chitosan; glutamic acid; hemagglutination; hemagglutinins; immunopathology; pH; public health; recombinant proteins; secondary immunization; viral antigens
Abstract:
... Avian influenza viruses (AIVs) and especially highly pathogenic (HP) AIVs of the H5 and H7 subtypes are of both veterinary and public health concern worldwide. In response to the demand for effective vaccines against H5N1 HPAIVs, we produced recombinant protein based on hemagglutinin (HA), a protective viral antigen. A fragment of the HA ectodomain, with a multibasic cleavage site deletion, was ex ...
DOI:
10.1016/j.vetimm.2022.110406

11. Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-α, IL1-β, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-γ bovine IL-17A and IL-1β revealed they recognize epitopes conserved on dolphin and bovine orthologues

Author:
Mahmoud M. Elnaggar; Gaber S. Abdellrazeq; Randy E. Sacco; Trevor R. Harsla; Mallory L. Mucci; Lindsay M. Fry; Victoria Hulubei; William C. Davis
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110456
ISSN:
0165-2427
Subject:
Tursiops truncatus; cattle; dolphins; epitopes; evolution; immunization; immunopathology; indicator species; interleukin-10; interleukin-17; interleukin-6; interleukin-8; leukocytes; sheep
Abstract:
... Opportunities to include Cetancodontamorpha in the study of the evolution of the immune system in the clades of Artiodactylamorpha, Ruminantiamorpha, Suinamorpha, and Camelidamorpha have increased with the use of the bottlenose dolphin, Tursiops truncatus, as a sentinel species to study the effects of environmental pollutants on the health of marine mammals. Efforts are currently underway to incre ...
Handle:
10113/7803613
DOI:
10.1016/j.vetimm.2022.110456

12. Controlled release vaccine implants for delivery of booster immunisations

Author:
Freya A. Russell; Logan K. Trim; Flavia Medeiros Savi; Craig Simon; Tim R. Dargaville; Dietmar W. Hutmacher; Kenneth W. Beagley
Source:
Veterinary immunology and immunopathology 2022 v.253 pp. 110484
ISSN:
0165-2427
Subject:
T-lymphocytes; antibodies; antigens; blood serum; cell proliferation; immune response; immunopathology; livestock; polymers; secondary immunization; subcutaneous injection; tetanus; vaccination; vaccines; weight gain
Abstract:
... Most current animal vaccine regimes involve a primary vaccination followed sometime later by a booster vaccination. This presents challenges when vaccinating difficult to access animals such as livestock. Mustering livestock to deliver a vaccine boost is costly and stressful for animals. Thus, we have produced a platform system that can be administered at the same time as the priming immunisation ...
DOI:
10.1016/j.vetimm.2022.110484

13. Detection of PRRSV-2 alone and co-localized with CD163 positive macrophages in porcine placental areolae

Author:
Javier Barrera-Zarate; Susan E. Detmer; J. Alex Pasternak; Glenn Hamonic; Daniel J. MacPhee; John C.S. Harding
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110457
ISSN:
0165-2427
Subject:
Betaarterivirus suid 2; embryogenesis; endometrium; fetus; fluorescent antibody technique; immunopathology; inflammation; macrophages; phenotype; placenta; pregnancy; transplacental transmission; viral load; viruses
Abstract:
... The porcine epitheliochorial placenta creates a barrier for the transplacental transfer of some nutrients from the dam to the fetus, as well as feto-lethal viruses such as porcine reproductive and respiratory syndrome virus 2 (PRRSV-2). Areolae are specialized structures within the porcine placenta with a high absorptive and substance transport capacity that facilitate embryonic development. The o ...
DOI:
10.1016/j.vetimm.2022.110457

14. Development of low-cost cage-like particles to formulate veterinary vaccines

Author:
Giuliana A. Lupi; Florencia X. Santiago Valtierra; Gabriel Cabrera; Roque Spinelli; Álvaro S. Siano; Verónica González; Antonio Osuna; Gerardo M. Oresti; Iván Marcipar
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110460
ISSN:
0165-2427
Subject:
adjuvants; alum; cholesterol; delayed hypersensitivity; egg yolk; gas chromatography; immunoglobulin G; immunopathology; lysophospholipids; ovalbumin; saponins; thin layer chromatography
Abstract:
... Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extracti ...
DOI:
10.1016/j.vetimm.2022.110460

15. Diagnostic accuracy of digital Brix and serum total protein refractometers in estimating different passive immunity levels in dairy calves

Author:
Mehmet Akköse; Hüseyin Gökhan Kutsal; Mert Kurban; Elif Merve Çinar; Yadigar Polat; Murat Cengiz
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110442
ISSN:
0165-2427
Subject:
Holstein; blood serum; brix; immunopathology; passive immunity; refractive index
Abstract:
... The aim of this study was to determine the diagnostic accuracy of digital serum total protein (TP) and digital Brix refractometers in estimating different passive immunity levels (<10, <18, <25 mg/mL) in dairy calves. Blood samples were collected from 260 apparently healthy Holstein calves, aged 2–7 days. Serum IgG concentrations were measured using digital Brix and TP refractometers and the radia ...
DOI:
10.1016/j.vetimm.2022.110442

16. Differential detection of IgM and IgG antibodies to chimeric antigens in bovine tuberculosis

Author:
Archana A. Sridhara; Ashley Johnathan-Lee; Rubyat Elahi; Paul Lambotte; Javan Esfandiari; Maria Laura Boschiroli; Tanya J. Kerr; Michele A. Miller; Thomas Holder; Gareth Jones; H. Martin Vordermeier; Breanne N. Marpe; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Konstantin P. Lyashchenko
Source:
Veterinary immunology and immunopathology 2022 pp. 110499
ISSN:
0165-2427
Subject:
Mycobacterium bovis; antibodies; blood serum; bovine tuberculosis; cattle; cross reaction; hosts; immunopathology; monitoring; rapid methods; risk; wildlife; France; Great Britain; South Africa
Abstract:
... Recent studies have suggested the potential of innovative serologic tests for accurate and rapid detection of bovine tuberculosis (bTB). Dual Path Platform (DPP) technology has been used to develop rapid animal-side antibody tests for Mycobacterium bovis infection in a range of livestock and wildlife host species. The present study evaluated diagnostic performance of DPP BovidTB IgM/IgG assay desi ...
DOI:
10.1016/j.vetimm.2022.110499

17. Distribution of M1 and M2 macrophages in cerebral granulomas caused by Encephalitozoon cuniculi

Author:
Adolfo Soto-Domínguez; Jennifer A. González-Castillo; Uziel Castillo-Velázquez; Luis E. Rodriguez-Tovar; Gerardo Méndez-Zamora; Diana E. Zamora-Avila; Alicia M. Nevárez-Garza
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110481
ISSN:
0165-2427
Subject:
Encephalitozoon cuniculi; brain; granuloma; immunology; immunopathology; inflammation; macrophages; phenotype
Abstract:
... Encephalitozoon cuniculi spores cause severe granulomatous inflammation in the brain where mononuclear cells and macrophages infiltrate. Here, we orally infected New Zealand white rabbits with 1 × 10⁶E. cuniculi viable spores to study the recruitment and localization of macrophages in brain granulomas. At day 30 post-infection, the positive phenotype markers iNOS (M1) and Arg-1 (M2) were located i ...
DOI:
10.1016/j.vetimm.2022.110481

18. Dynamic changes in Holstein heifer circulatory stress biomarkers in response to lipopolysaccharide immune challenge

Author:
T. Sullivan; A. Sharma; K. Lamers; C. White; B.A. Mallard; A. Cánovas; N.A. Karrow
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110426
ISSN:
0165-2427
Subject:
Holstein; acidosis; biomarkers; blood flow; blood serum; body temperature; climate change; cortisol; heat stress; immunopathology; inflammation; interleukin-10; intestinal mucosa; leukopenia; lipopolysaccharides; microRNA; septicemia; stress response; thrombocytopenia
Abstract:
... Dairy cattle routinely face a variety of stressors. For example, climate change has resulted in more frequent heat stress events that increase the incidence of bacterial infections by inducing conditions like leaky gut syndrome, whereby the integrity of the intestinal epithelium is compromised allowing for luminal bacteria and their membrane components to infiltrate the host’s bloodstream resultin ...
DOI:
10.1016/j.vetimm.2022.110426

19. Effect of Holstein genotype on ex-vivo cytokine response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) during the periparturient period

Author:
Amber A. Brink; Wanda J. Weber; John D. Lippolis; John B. Cole; Sandra M. Godden; Anthony Seykora; Brian A. Crooker
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110463
ISSN:
0165-2427
Subject:
Holstein; energy balance; genotype; immunopathology; innate immunity; interleukin-6; lactation; lipopolysaccharides; lipoteichoic acids; milk; pathogen-associated molecular patterns
Abstract:
... Effects of Holstein genotype on innate immune response were assessed with ex-vivo lipopolysaccharide (LPS) and lipoteichoic acid (LTA) stimulation of whole blood from unselected (UH, n = 10) and contemporary (CH, n = 11) Holsteins that differ in production by more than 4,500 kg/lactation. Blood was collected at −14, 7, 28, and 49 days in milk (DIM), mixed with a pathogen-associated molecular patte ...
Handle:
10113/7837475
DOI:
10.1016/j.vetimm.2022.110463

20. Effect of In-vivo heat challenge on physiological parameters and function of peripheral blood mononuclear cells in immune phenotyped dairy cattle

Author:
S.L. Cartwright; J. Schmied; A. Livernois; B.A. Mallard
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110405
ISSN:
0165-2427
Subject:
barns; body temperature; cell proliferation; cell respiration; climate change; dairy cattle; disease incidence; heat; heat stress; heat tolerance; heat-shock protein 70; humidity; immunology; immunopathology; vaccines
Abstract:
... The frequency of heat waves and hot days are increasing due to climate change, which leads to an increase in the occurrence of heat stress in dairy cattle. Previous studies have shown that dairy cattle identified as high immune responders have a reduced incidence of disease and improved vaccine response compared to average and low responders. Additionally, it has been observed that when cells from ...
DOI:
10.1016/j.vetimm.2022.110405

21. Effect of aging on monocyte phagocytic and inflammatory functions, and on the ex vivo inflammatory responses to lipopolysaccharide, in horses

Author:
A.C. Herbst; S.E. Reedy; A.E. Page; D.W. Horohov; A.A. Adams
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110459
ISSN:
0165-2427
Subject:
Escherichia coli; adiposity; body condition; ex vivo studies; flow cytometry; gene expression; genes; immunopathology; interleukin-18; interleukin-6; interleukin-8; lipopolysaccharides; monocytes; phagocytosis; young adults
Abstract:
... Studies investigating age-related changes in the function of monocytes are currently limited for horses. Thus, the main goal of this study was to determine the effect of aging on monocyte phagocytic capacity and pro-inflammatory cytokine responses to bacterial lipopolysaccharide (LPS). A second goal of this work was to examine the effect of aging on the inflammatory cytokine responses to LPS in a ...
DOI:
10.1016/j.vetimm.2022.110459

22. Effect of major versus minor mastectomy on host immunity in canine mammary cancer

Author:
Maria Karayannopoulou; Tilemachos Anagnostou; Apostolia Margariti; Maria Kritsepi-Konstantinou; Dimitra Psalla; Angelos-Lauris Thomas; Ioannis Savvas
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110403
ISSN:
0165-2427
Subject:
T-lymphocytes; anesthesia; cell-mediated immunity; immunopathology; immunosuppression; innate immunity; mammary neoplasms (animal); metastasis; neutrophils; surgery
Abstract:
... Surgical procedures can affect host immunity proportionally to the extent of surgical trauma. In cancer cases, surgery-induced immunosuppression can potentially promote tumour metastasis. The aim of this study was to investigate, in bitches with malignant mammary tumours, whether major surgery (total unilateral mastectomy or bilateral regional mastectomy) has a more negative effect than minor surg ...
DOI:
10.1016/j.vetimm.2022.110403

23. Effects of frequent teat stimulation on antimicrobial component production in mammary glands of lactating goats

Author:
Yusaku Tsugami; Rika Harada; Takahiro Nii; Naoki Suzuki; Naoki Isobe
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110431
ISSN:
0165-2427
Subject:
Japan; calcium-binding proteins; epithelium; humans; immunopathology; keratinocytes; lactoferrin; milk; milk yield
Abstract:
... Various antimicrobial components, such as lactoferrin, S100 calcium-binding protein A7 (S100A7), and IgA, produced by epithelial cells and leukocytes in lactating mammary glands are important for host defense against invading pathogens. Increase in milking frequency enhances milk yield in ruminants and implies an increase in frequency of teat stimulation. However, the influence of frequent teat st ...
DOI:
10.1016/j.vetimm.2022.110431

24. Effects of immunostimulators of microbial origin on T cells of pigs vaccinated with attenuated vaccine against Aujeszkyʼs disease

Author:
Miroslav Andrišić; Irena Žarković; Ksenija Šandor; Anja Vujnović; Eleonora Perak Junaković; Krešo Bendelja; Ana Savić Mlakar; Nada Oršolić; Lidija Šver; Miroslav Benić; Svjetlana Terzić
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110365
ISSN:
0165-2427
Subject:
Escherichia coli; Parapoxvirus; Propionibacterium granulosum; Suid alphaherpesvirus 1; T-lymphocytes; cell-mediated immunity; immunopathology; live vaccines; vaccination
Abstract:
... Aujeszky’s disease (AD) is a viral infectious disease caused by Suid herpesvirus 1 (SuHV-1). Vaccination and eradication of AD in domestic pigs is possible using marker vaccines with attenuated or inactivated SuHV-1, or subunit vaccines. However, vaccines with attenuated SuHV-1 have shown to be more potent in inducing strong cell-mediated immune response. The studies have shown that Parapoxvirus o ...
DOI:
10.1016/j.vetimm.2021.110365

25. Effects of intra-articular inoculation with Mycoplasma bovis on immunological responses in calf joints

Author:
Koji Nishi; Yuki Hirano; Ayano Sato; Ayako Eguchi; Kazuya Matsuda; Miyuki Toda; Takafumi Watanabe; Tomohito Iwasaki; Naoki Takahashi; Marina Hosotani; Reina Watanabe; Toshihide Kato; Hiromichi Ohtsuka; Satoshi Gondaira; Hidetoshi Higuchi
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110364
ISSN:
0165-2427
Subject:
Mycoplasma bovis; arthritis; calves; cartilage; fibrin; immunohistochemistry; immunopathology; inflammation; interleukin-12; interleukin-17; interleukin-6; interleukin-8; lameness; messenger RNA; monitoring; phosphates; stifle; synovial fluid; transmission electron microscopy
Abstract:
... Mycoplasma arthritis that caused by Mycoplasma bovis exhibit severe lameness. This disease is difficult to cure with antibiotics, but the detailed pathological mechanisms have not been fully clarified. In this study, we examined the effects of intra-articular inoculation with M. bovis on immunological responses in calf joints. We inoculated three calves each with M. bovis or phosphate buffer salin ...
DOI:
10.1016/j.vetimm.2021.110364

26. Evaluating the Immunogenicity of Recombinant VP1 Protein from the Foot-and-Mouth Disease Virus Encapsulated in Nanoliposome in Guinea Pig Animal Model

Author:
Marjan Kazemi; Mahmoud Reza Aghamaali; Rasool Madani; Tara Emami; Fariba Golchinfar
Source:
Veterinary immunology and immunopathology 2022 pp. 110497
ISSN:
0165-2427
Subject:
Foot-and-mouth disease virus; adjuvants; animal models; blood serum; cholesterol; foot-and-mouth disease; genes; guinea pigs; immune response; immunogenicity; immunopathology; livestock; neutralization; viruses
Abstract:
... Foot-and-mouth disease (FMD) is considered as contagious in livestock, which is caused by the Picornavridae virus family known as FMD virus (FMDV). In the present study, the VP1 gene from FMDV (O strain) was expressed and purified. In addition, nanoliposomes were utilized as an adjuvant. After formulating the nanoliposomes with DMPC, DMPG, and cholesterol, the recombinant VP1 protein was encapsula ...
DOI:
10.1016/j.vetimm.2022.110497

27. Evaluation of PD-1 and PD-L1 expression in canine urothelial carcinoma cell lines

Author:
Christopher J. Pinard; Samuel E. Hocker; Andrew C. Poon; Jordon M. Inkol; Arata Matsuyama; R. Darren Wood; Geoffrey A. Wood; J. Paul Woods; Anthony J. Mutsaers
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110367
ISSN:
0165-2427
Subject:
Western blotting; carcinoma; cell lines; dogs; flow cytometry; fluorescence microscopy; gene expression; humans; immunopathology; immunotherapy; neoplasm cells; reverse transcriptase polymerase chain reaction
Abstract:
... Urothelial carcinoma (UC) is the most common urinary tumor in dogs and despite combinational therapies, only modest improvements in survival have been achieved in recent years. Given the utility of monoclonal antibodies against PD-1 and PD-L1 in human UC, we evaluated the protein and mRNA expression in three established canine urothelial carcinoma cell lines. Flow cytometry and western blot analys ...
DOI:
10.1016/j.vetimm.2021.110367

28. Evaluation of equine xenogeneic mixed lymphocyte reactions using 5-ethynyl-2’-deoxyuridine (EdU)

Author:
Kate E. Birdwhistell; David J. Hurley; Bradley Heins; John F. Peroni
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110430
ISSN:
0165-2427
Subject:
T-lymphocytes; cell proliferation; cytokines; flow cytometry; horses; immunopathology; nucleobases; organ transplantation; swine; thymidine
Abstract:
... Allogeneic solid organ transplantation is currently the only treatment option for end stage organ disease. The shortage of available donor organs has driven efforts to utilize xenogeneic organs for transplantation. In vitro methods for evaluating immune-compatibility are a quick and low cost means of screening novel tissue products prior to more involved, expensive, and invasive live animal studie ...
DOI:
10.1016/j.vetimm.2022.110430

29. Evaluation of peripheral blood polymorphonuclear cell functions after an oral carbohydrate overload in obese and insulin dysregulated horses

Author:
Constanza Salinas; Gabriel Espinosa; Natalia Morales; Claudio Henríquez; Gabriel Morán; Gonzalo Gajardo; Rafael A. Burgos; Benjamin Uberti
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110455
ISSN:
0165-2427
Subject:
adults; apoptosis; blood; blood glucose; body condition; glucose; hyperglycemia; immunopathology; insulin; insulin resistance; obesity; phagocytosis; reactive oxygen species; statistical models
Abstract:
... Obesity and insulin dysregulation (ID) are increasingly prevalent conditions in equid populations worldwide. Immune impairment is well described in humans with metabolic dysfunction and is reported but still incompletely understood in horses. This study evaluated the effect of acute induced transient hyperglycemia on apoptosis, phagocytosis and oxidative burst activity of peripheral blood polymorp ...
DOI:
10.1016/j.vetimm.2022.110455

30. Evaluation of the efficacy of cow colostrum in the treatment and its effect on serum proteomes in calves with cryptosporidiosis

Author:
Yiğit KAÇAR; Ahmet Tarık BAYKAL; Levent AYDIN; Hasan BATMAZ
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110429
ISSN:
0165-2427
Subject:
Cryptosporidium; Holstein; amyloid; blood serum; calves; cow colostrum; cryptosporidiosis; gamma-glutamyltransferase; immunoglobulin G; immunopathology; oocysts; protein content; proteome; proteomics; sodium bicarbonate; zinc
Abstract:
... This study aimed to investigate the effect of using high-quality colostrum in addition to paromomycin on the treatment outcomes and serum proteomes of calves naturally affected by cryptosporidiosis. Thirty Holstein calves infected with only Cryptosporidium spp. were divided into three equal groups. Calves in the PC group received paromomycin orally at a dose of 100 mg/kg once daily for 5 days. Cal ...
DOI:
10.1016/j.vetimm.2022.110429

31. Evidence for transfer of maternal antigen specific cellular immunity against Mycobacterium avium ssp. paratuberculosis via colostrum in a goat twin model

Author:
L. Robbers; R. van de Mheen; L. Benedictus; R. Jorritsma; M. Nielen; H.J.C. Bijkerk; S.G. van der Grein; L. Ravesloot; A.P. Koets
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110402
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; blood serum; cell-mediated immunity; cow colostrum; epithelium; histology; immune response; immunopathology; injection site; intestines; models; tuberculin; vaccines
Abstract:
... Colostrum intake is one of the most important factors in neonatal health in ruminants, mainly because of its unique immunological properties. Both in practice as well as in research, the attention of lactogenic immunity is focused on the importance of colostral antibodies and less attention is given to the functional role of maternal cells in colostrum. Here we study the transfer of maternal leuko ...
DOI:
10.1016/j.vetimm.2022.110402

32. Ex vivo activated CD4+ T cells from young calves exhibit Th2-biased effector function with distinct metabolic reprogramming compared to adult cows

Author:
Jordan M. Eder; Randy E. Sacco
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110418
ISSN:
0165-2427
Subject:
acidification; adults; gene expression; glycolysis; immunopathology; interleukin-4; mitochondria; oxygen consumption; passive immunity; phenotype
Abstract:
... As maternal passive immunity wanes at 6–8 weeks, young calves must rely on their own naïve and developing immune system for protection against pathogens. Typically, an infection in the young induces a T cell-mediated response, which skews towards a Th2 phenotype and results in a reduced effector response. Our study examines the implications this transitional period of immunocompetency has on cellu ...
DOI:
10.1016/j.vetimm.2022.110418

33. Field evaluation of the tuberculin skin test for the detection of Mycobacterium tuberculosis complex infection in communal goats (Capra hircus) in KwaZulu-Natal, South Africa

Author:
Deborah M. Cooke; Wynand J. Goosen; Carmel Witte; Michele A. Miller
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110486
ISSN:
0165-2427
Subject:
Capra hircus; Mycobacterium tuberculosis complex; Saanen; Syncerus caffer; cattle; dairy herds; goats; immunopathology; land reform; pathogens; risk; rural development; tuberculin; tuberculosis; South Africa
Abstract:
... In South Africa, animal tuberculosis (TB) control programs predominantly focus on domestic cattle and African buffaloes (Syncerus caffer) despite increasing global reports of tuberculosis in goats (Capra hircus). Left undetected, Mycobacterium tuberculosis complex (MTBC) infected goats may hinder TB eradication efforts in cattle and increase zoonotic risk to humans. Since the publication of animal ...
DOI:
10.1016/j.vetimm.2022.110486

34. Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes

Author:
S. Casaro; M.G. Marrero; D.M.C. Madrid; J.G. Prim; C.D. Nelson; K.N. Galvão; J. Laporta; J.P. Driver
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110417
ISSN:
0165-2427
Subject:
Holstein; calves; dairy cows; flow cytometry; immunopathology; immunophenotyping; monocytes; phenotype; pregnancy
Abstract:
... Many aspects of the bovine immune system remain poorly characterized, which poses an obstacle to improving dairy cow health. Herein, we describe two flow cytometry panels that included antibodies against CD8α, CD4, TCR-δ, CD172α, CD14, MHCII, CD21, CD62L, and CD11b. These panels were used to characterize the phenotype of leukocyte subpopulations from the peripheral blood of 30-day old Holstein cal ...
DOI:
10.1016/j.vetimm.2022.110417
CHORUS:
10.1016/j.vetimm.2022.110417

35. H3N2 canine influenza virus NS1 protein inhibits canine NLRP3 inflammasome activation

Author:
Pan Tao; Zhangyong Ning; Pei Zhou; Weiqi Xiao; Guiping Wang; Shoujun Li; Guihong Zhang
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110483
ISSN:
0165-2427
Subject:
Influenza A virus; canine influenza; caspase-1; dogs; immunopathology; inflammasomes; inflammation; innate immunity; interleukin-18; oligomerization; transcription (genetics)
Abstract:
... Inflammation is an innate immune response of the body against pathogens and other irritants. The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome is a major player in the inflammatory response against pathogenic microorganisms. In this study, we analyzed the relationship between the NLRP3 inflammasome and the influenza virus NS1 protein, which is involved in host immune escape. ...
DOI:
10.1016/j.vetimm.2022.110483

36. Identification of EDIL3 biomarkers as a biomarker and potential therapeutic target of canine mammary carcinomas based on integrated bioinformatics analysis

Author:
Zixiang Lin; Ce Jiang; Di Lv; Degui Lin
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110432
ISSN:
0165-2427
Subject:
bioinformatics; biomarkers; carcinogenesis; data collection; dogs; genes; immunity; immunopathology; mammary neoplasms (animal); therapeutics
Abstract:
... As the fierce battle with cancer is now expanding to companion animals, effective treatment of canine mammary carcinomas (CMT), as the most frequently diagnosed tumor in intact dogs, is becoming a crucial issue. Although many studies have been carried out concerning the clinical application of mammary tumor biomarkers, no ideal biomarker has yet been identified in CMT. Therefore, in this work, we ...
DOI:
10.1016/j.vetimm.2022.110432

37. Immunoproteomic analysis of the secretome of bovine-adapted strains of Staphylococcus aureus demonstrates a strain-specific humoral response

Author:
Shauna D. Drumm; Paul Cormican; Padraig O’Boyle; Rebecca A. Owens; Jennifer Mitchell; Orla M. Keane
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110428
ISSN:
0165-2427
Subject:
Staphylococcus aureus; antigens; blood serum; cows; genes; humoral immunity; immunopathology; mass spectrometry; molecular weight; pathogens; vaccines; virulence
Abstract:
... Staphylococcus aureus is a common pathogen associated with bovine intramammary infection. A number of distinct S. aureus lineages are associated with such infections although there is a dearth of knowledge regarding the major immunogenic antigens associated with each lineage and whether these antigens provide protection against heterologous strains. Identification of the major immunogenic antigens ...
DOI:
10.1016/j.vetimm.2022.110428

38. Infection by bovine alphaherpesvirus types 1 and 5 induces IFN-λ3 expression in neuronal-type cells and bovine neural tissues

Author:
J.J. Rosales; M.V. Nieto Farías; M. Burucúa; M.S. Marin; S.E. Pérez
Source:
Veterinary immunology and immunopathology 2022 v.245 pp. 110391
ISSN:
0165-2427
Subject:
Bovine alphaherpesvirus 1; bioactive properties; brain; epithelium; ganglia; immunopathology; innate immunity; transcription (genetics); virus replication; viruses
Abstract:
... Type III interferons (IFNs) are components of the innate immunity, with IFN lambda- (λ)3 having the most potent bioactivity in humans. IFN-λ has a predominant role in epithelial cells. However, antiviral function in certain infections of the central nervous system has also been demonstrated. IFN-λ3 expression in neural tissues of cattle has not been investigated. Thus, the aim of this study was to ...
DOI:
10.1016/j.vetimm.2022.110391

39. Influenza A pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines

Author:
Jiae Lee; Yeojin Hong; Thi Hao Vu; Sooyeon Lee; Jubi Heo; Anh Duc Truong; Hyun S. Lillehoj; Yeong Ho Hong
Source:
Veterinary immunology and immunopathology 2022 v.246 pp. 110404
ISSN:
0165-2427
Subject:
Influenza A virus; RNA; chickens; cytokines; gene expression; gene expression regulation; immune system; immunity; immunopathology; influenza; interferons; quantitative polymerase chain reaction; reverse transcription; viruses; Vietnam
Abstract:
... Influenza A/H5N1 virus is a highly pathogenic (HPAIV) and contagious zoonotic virus that can be transmitted to humans. In the present study, infection with this virus and differential gene expression analyses were carried out with genetically resistant and susceptible Ri chicken lines that are native to Vietnam. A total of 20 four-week-old Ri chickens from each line were inoculated with the highly ...
DOI:
10.1016/j.vetimm.2022.110404

40. Insufficiency of CD205-positive cortical thymic epithelial cells in immature Japanese Black cattle with severe thymic abnormalities and poor prognosis

Author:
Shoichi Wakitani; Risako Kawabata; Masahiro Yasuda
Source:
Veterinary immunology and immunopathology 2022 v.245 pp. 110379
ISSN:
0165-2427
Subject:
Wagyu; cortex; epithelium; histopathology; immunohistochemistry; immunopathology; interleukin-7; prognosis; surveys
Abstract:
... To investigate the involvement of thymic function in the development of diseases with poor prognosis in calves, this study conducted a survey for the assessment of thymus cell composition in immature Japanese Black cattle with poor prognosis. Histopathological evaluation of 47 cattle showed signs of acute thymic involution in most cases. Less than half of the cases had a cortex predominant over th ...
DOI:
10.1016/j.vetimm.2021.110379

41. Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages

Author:
Shingo Ishikawa; Masataka Miyazawa; Chiho Tanaka; Ryoma Uesawa; Juri Nishizawa; Ryoko Uemura; Ikuo Kobayashi; Seiji Hobo
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110378
ISSN:
0165-2427
Subject:
bovine respiratory disease; cattle; gene expression; immunopathology; inducible nitric oxide synthase; interferon-gamma; lipopolysaccharides; macrophages; pathogenesis; polyinosinic-polycytidylic acid; sodium; therapeutics; tumor necrosis factor-alpha
Abstract:
... To understand the pathogenesis of bovine respiratory disease (BRD), it is necessary to elucidate the mechanisms of alveolar macrophage regulation by cytokines and pathogen-associated molecular patterns (PAMPs). Moreover, "non-specific effects (NSEs)” an innate immune regulatory mechanism in response to vaccines containing PAMPs, has recently attracted attention. It may be applied to BRD control, b ...
DOI:
10.1016/j.vetimm.2021.110378

42. Interleukin-1Ra (Il-1Ra) and serum cortisol level relationship in horse as dynamic adaptive response during physical exercise

Author:
Francesca Arfuso; Elisabetta Giudice; Michele Panzera; Maria Rizzo; Francesco Fazio; Giuseppe Piccione; Claudia Giannetto
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110368
ISSN:
0165-2427
Subject:
Thoroughbred; antagonists; athletic performance; blood serum; cortisol; erythrocytes; exercise; hematocrit; hemoglobin; horses; immunopathology; interleukin-1; physical health; stress response
Abstract:
... The monitoring of endocrine and immunologic markers during exercise is of paramount importance to assess and/or maintain the physical well-being of athletes as well as to optimize the athletic performance. This study aimed to investigate the linkage between acute stress response and immune status in Thoroughbred horses competing in an official 1300-m race. From 10 horses blood was collected 1 week ...
DOI:
10.1016/j.vetimm.2021.110368

43. Low molecular weight heparin reduces acute phase response and multiple organ dysfunction following Ovine experimental endotoxemia model

Author:
Rouhollah Esmaeili Seraji; Aliasghar Chalmeh; Mehrdad Pourjafar
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110361
ISSN:
0165-2427
Subject:
Escherichia coli; amyloid; anti-inflammatory agents; aspartate transaminase; bilirubin; biomarkers; blood serum; creatine kinase; creatinine; death; dose response; endotoxemia; glutathione peroxidase; heparin; homocysteine; immunopathology; intravenous injection; lipopolysaccharides; models; oxidative stress; serotypes; superoxide dismutase; tumor necrosis factor-alpha
Abstract:
... Endotoxemia is one of the most common inflammatory situations leading to death of ruminants. Owing to the importance of this condition, several therapeutic regimens have been proposed, evaluated and implemented to treat endotoxemia. It has recently been suggested that low molecular weight heparin may be effective in treating endotoxemia. Thus, the present experimental study was conducted to evalua ...
DOI:
10.1016/j.vetimm.2021.110361

44. Measurement of canine Th17 cells by flow cytometry

Author:
A. Knebel; A. Kämpe; R. Carlson; K. Rohn; A. Tipold
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110366
ISSN:
0165-2427
Subject:
CD4-positive T-lymphocytes; autoimmune diseases; dogs; flow cytometry; immunology; immunopathology; inflammation
Abstract:
... Th17 cells are T helper cells which play an important role during inflammation and autoimmune disease. To investigate the role of these cells in diseases in dogs in a clinical setting, methods for fast identification had to be established. Th17 cells are a rare cell population, for their measurement stimulation is recommended. To examine more samples simultaneously and to receive a relatively high ...
DOI:
10.1016/j.vetimm.2021.110366

45. Oxidative stress in neonatal lambs and its relation to health status and passive colostral immunity

Author:
Erhan Gökçe; Pınar Cihan; Onur Atakişi; Ali Haydar Kirmizigül; Hidayet Metin Erdoğan
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110470
ISSN:
0165-2427
Subject:
antioxidant activity; blood; colostral immunity; colostrum; crossbreds; health status; immunopathology; ingestion; oxidants; oxidative stress; postpartum period
Abstract:
... Oxidative stress (OS) related to birth in ruminants may increase susceptibility to diseases. However, information about the physiological changes related to OS in lambs is limited. This study was designed to evaluate changes in OS parameters [total antioxidant status (TAS, mmol Trolox Equiv./L), total oxidant status (TOS, μmol H₂O₂ Equiv./L)] and calculated oxidative stress index (OSi = TOS/TAS *  ...
DOI:
10.1016/j.vetimm.2022.110470

46. Pharmacological inhibition of the mTOR pathway alters phenotype and cytokine expression in bovine monocyte-derived dendritic cells

Author:
Anja Sipka; Thomas Weichhart; Sabine Mann
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110441
ISSN:
0165-2427
Subject:
Escherichia coli; antigen presentation; cell culture; early lactation; flow cytometry; gene expression; immune response; immunopathology; infectious diseases; inflammation; interleukin-10; lipopolysaccharides; monocytes; nutrient availability; pathogens; phenotype; rapamycin
Abstract:
... Epidemiological studies have long demonstrated the association of nutrient status and immune dysfunction in dairy cows. Postpartum dairy cows experiencing a nutrient deficit show a propensity for increased inflammatory response, decreased pathogen clearance, and increased incidence of infectious disease. Studies in cows and other species show that the nutrient sensing mechanistic target of rapamyc ...
DOI:
10.1016/j.vetimm.2022.110441

47. Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms

Author:
Archana A. Sridhara; Ashley Johnathan-Lee; Rubyat Elahi; Alina Sikar-Gang; Paul Lambotte; Javan Esfandiari; Lucia de Juan; Christian Gortazar; Breanne N. Marpe; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Konstantin P. Lyashchenko
Source:
Veterinary immunology and immunopathology 2022 v.248 pp. 110419
ISSN:
0165-2427
Subject:
Mycobacterium bovis; antibodies; biomarkers; blood; bovine tuberculosis; cattle; diagnostic sensitivity; immunopathology; interferon-gamma; tuberculin; Spain
Abstract:
... Bovine tuberculosis (bTB) control programs can be improved by combined use of tests for humoral and cell-mediated immune responses targeting multiple biomarkers of Mycobacterium bovis. To further the diagnostic benefits of this approach, we used Dual Path Platform (DPP) technology to test sera from cattle with naturally acquired bTB in the United States (US) and Spain for the presence of M. bovis ...
DOI:
10.1016/j.vetimm.2022.110419

48. Protective effects of chicoric acid on polyinosinic-polycytidylic acid exposed chicken hepatic cell culture mimicking viral damage and inflammation

Author:
Patrik Tráj; Eva Madeleine Herrmann; Csilla Sebők; Júlia Vörösházi; Máté Mackei; Péter Gálfi; Ágnes Kemény; Zsuzsanna Neogrády; Gábor Mátis
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110427
ISSN:
0165-2427
Subject:
Asteraceae; acetylcysteine; biochemical pathways; chickens; coculture; culture media; dose response; double-stranded RNA; free radicals; immunopathology; inflammation; interleukin-6; interleukin-8; lactate dehydrogenase; polyinosinic-polycytidylic acid; poultry industry; viruses
Abstract:
... Virus induced damage triggered by excessive inflammation and free radical production is a major threat in the poultry industry, leading to low productivity even in vaccinated flocks. The purpose of the study was to induce inflammation with the viral double-stranded RNA analog polyinosinic-polycytidylic acid (poly I:C) on chicken primary hepatocyte – non-parenchymal cell co-cultures to investigate ...
DOI:
10.1016/j.vetimm.2022.110427

49. Reduced capability of refrigerated white rhinoceros whole blood to produce interferon-gamma upon mitogen stimulation

Author:
Rebecca Dwyer; Carmel Witte; Peter Buss; Tebogo Manamela; Leana Freese; Guy Hausler; Wynand J. Goosen; Michele Miller
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110485
ISSN:
0165-2427
Subject:
Ceratotherium simum; Mycobacterium bovis; absorbance; blood; immunopathology; interferon-gamma; males; mitogens; monitoring; national parks; refrigeration
Abstract:
... Ante-mortem surveillance for Mycobacterium bovis (M. bovis) infection in the Kruger National Park (KNP) rhinoceros population currently relies on results from the QuantiFERON-TB Gold (In-Tube) Plus (QFT)–interferon gamma (IFN-γ) release assay (IGRA). However, same-day processing of rhinoceros blood samples for this test is a logistical challenge. Therefore, a pilot study was performed to compare m ...
DOI:
10.1016/j.vetimm.2022.110485

50. Residue of thiram in food, suppresses immune system stress signals and disturbs sphingolipid metabolism in chickens

Author:
Yingwei Liu; Jiangnan Yi; Yuanliang Li; Riaz Hussain; Shanshan Zhu; Yangwei Li; Zhuanxu Ouyang; Khalid Mehmood; Lianmei Hu; Jiaqing Pan; Zhaoxin Tang; Ying Li; Hui Zhang
Source:
Veterinary immunology and immunopathology 2022 v.247 pp. 110415
ISSN:
0165-2427
Subject:
angiogenesis; apoptosis; autophagy; cell membranes; immunology; immunopathology; immunotoxicity; metabolites; metabolome; necropsy; pollution; public health; sphingolipids; spleen; sulfur; thiram
Abstract:
... Thiram, a well-known sulfur containing organic compound is frequently and extensively used in agriculture because of high biological activity to control different pests. In certain cases, due to long persistence in the environment pesticides and other environmental contaminants induce undesirable toxic impacts to public health and environment. To ascertain the potential mechanisms of toxicity of t ...
DOI:
10.1016/j.vetimm.2022.110415

51. Rules of thumb to obtain, isolate, and preserve porcine peripheral blood mononuclear cells

Author:
Ivan Díaz
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110461
ISSN:
0165-2427
Subject:
T-lymphocytes; anticoagulants; cryopreservation; humans; immunity; immunopathology; swine; vaccination
Abstract:
... One of the most used biospecimens in immunology are peripheral blood mononuclear cells (PBMC). PBMC are particularly useful when evaluating immunity through responses of circulating B- and T-cells, during an infection, or after a vaccination. While several reviews and research papers have been published aiming to point out critical steps when sampling, isolating, and cryopreserving human PBMC -or ...
DOI:
10.1016/j.vetimm.2022.110461

52. Serological survey for maternal antibodies to Borreliella burgdorferi and Anaplasma phagocytophilum in dogs from endemic and non-endemic regions of the United States

Author:
Karen L. Stasiak; Nicholas A. Cramer; Gavin Z. Chambers; Richard T. Marconi
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110471
ISSN:
0165-2427
Subject:
Anaplasma phagocytophilum; Canine morbillivirus; Carnivore protoparvovirus 1; Lyme disease; anaplasmosis; antibodies; antigens; blood serum; colorimetry; dogs; immunopathology; serological surveys; seroprevalence; vaccination; vaccines
Abstract:
... In North America, the tick-borne pathogens Borreliella burgdorferi (Lyme disease; LD) and Anaplasma phagocytophilum (anaplasmosis) are a significant health threat to dogs. Little is known regarding the seroprevalence of maternal antibodies (Abs) to these pathogens in young dogs. The analysis of maternal antibody (Ab) profiles is important as it could bear on the interpretation of currently availab ...
DOI:
10.1016/j.vetimm.2022.110471

53. Sheep vaccinated against paratuberculosis have increased levels of B cells infiltrating the intestinal tissue

Author:
Hannah B. Pooley; Richard J. Whittington; Douglas J. Begg; Auriol C. Purdie; Karren M. Plain; Kumudika de Silva
Source:
Veterinary immunology and immunopathology 2022 v.252 pp. 110482
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; enteritis; ileum; immune response; immunohistochemistry; immunopathology; macrophages; paratuberculosis; sheep; vaccination; vaccine development; vaccines
Abstract:
... Systemic immunisation delivered subcutaneously is currently used to control paratuberculosis, a chronic enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). These vaccines do not provide complete protection and a small cohort of animals still succumb to clinical disease. The aim of this study was to assess mycobacterial infection site-specific variations in immun ...
DOI:
10.1016/j.vetimm.2022.110482

54. Systemic murine cathelicidin CRAMP safely attenuated colonic neutrophil infiltration in pigs

Author:
Cristina C. Fodor; Robert McCorkell; Greg Muench; Eduardo R. Cobo
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110443
ISSN:
0165-2427
Subject:
cathelicidins; colitis; colon; enteropathogens; epithelium; glycosylation; homeostasis; immunology; immunopathology; intraperitoneal injection; mice; mucins; mucus; multiple drug resistance; neutrophils
Abstract:
... Post-weaning diarrheic colitis, often caused by enteropathogens, are severe and potentially lethal diseases in young pigs. Conventional treatment with antibiotics is problematic due to increasing prevalence of multi-drug resistant bacteria. Few alternative treatments exist, so development of antibiotic-free therapies is urgently needed for livestock. Cathelicidin peptides, produced by epithelial c ...
DOI:
10.1016/j.vetimm.2022.110443

55. Taurine inhibits necroptosis helps to alleviate inflammatory and injury induced by Klebsiella infection

Author:
Huifang Yin; Zhenglei Wang; Shoushen Yang; Xintian Zheng; Yinli Bao; Weiming Lin; Cuiqin Huang; Longxin Qiu
Source:
Veterinary immunology and immunopathology 2022 v.250 pp. 110444
ISSN:
0165-2427
Subject:
Klebsiella; death; immunopathology; interleukin-6; necroptosis; phosphorylation; taurine
Abstract:
... Klebsiella infection is widely acknowledged to inflict severe inflammatory damage in bovines. Herein, we demonstrate significant death of EpH4-Ev cells incubated with Klebsiella. And compelling evidence shows that Klebsiella infection increases interactions between the Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) and RIPK3, which promotes phosphorylation of RIPK3 and MLKL to indu ...
DOI:
10.1016/j.vetimm.2022.110444

56. Thymic changes due to leishmaniasis in dogs: An immunohistochemical study

Author:
Giulia Gonçalves Jussiani; Karen Santos Março; Paulo Henrique Leal Bertolo; Rosemeri de Oliveira Vasconcelos; Gisele Fabrino Machado
Source:
Veterinary immunology and immunopathology 2022 v.247 pp. 110416
ISSN:
0165-2427
Subject:
Leishmania infantum; apoptosis; atrophy; collagen; death; epithelium; histopathology; immune response; immunohistochemistry; immunopathology; leishmaniasis; macrophages; mitosis; morphometry; parasites; pathophysiology; thymocytes; tissue distribution
Abstract:
... The thymus is necessary for the differentiation of T cells, a process that is regulated by the type of antigens found in thymocytes, the environment of surrounding cells and the thymus architecture. There is evidence that infectious diseases may result in morphological changes in this organ, such as premature atrophy and decreased thymocyte proliferation, that can affect the immune response. We ch ...
DOI:
10.1016/j.vetimm.2022.110416

57. Tracheal cellular immune response in chickens inoculated with Mycoplasma synoviae vaccine, MS-H or its parent strain 86079/7NS

Author:
Oluwadamilola S. Omotainse; Nadeeka K. Wawegama; Sathya N. Kulappu Arachchige; Mauricio J. C. Coppo; Paola K. Vaz; Andrew P. Woodward; Somayeh Kordafshari; Mirjana Bogeski; Mark Stevenson; Amir H. Noormohammadi; Andrew W. Stent
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110472
ISSN:
0165-2427
Subject:
Mycoplasma synoviae; cell-mediated immunity; immunopathology; interleukin-17; light microscopy; live vaccines; macrophages; mucosa; respiratory tract diseases; specific pathogen-free animals; vaccination
Abstract:
... Mycoplasma synoviae causes respiratory tract disease in chickens characterised by mild to moderate lymphoplasmacytic infiltration of the tracheal mucosa. MS-H (Vaxsafe1 MS, Bioproperties Pty Ltd.) is an effective live attenuated vaccine for M. synoviae, but the immunological basis for its mechanism of protection has not been investigated, and the phenotypes of lymphocytes and associated cytokines ...
DOI:
10.1016/j.vetimm.2022.110472

58. Transcriptome diversity and differential expression in supporting limb laminitis

Author:
Heather M. Holl; Caitlin Armstrong; Hannah Galantino-Homer; Samantha A. Brooks
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110353
ISSN:
0165-2427
Subject:
Thoroughbred; biomarkers; etiology; euthanasia; gene expression regulation; genes; hooves; hospitals; immunology; immunopathology; inflammasomes; inflammation; laminitis; leukocytes; quality of life; stress response; transcriptome
Abstract:
... Laminitis results in impaired tissue integrity and Inflammation of the epidermal and dermal lamellae connecting the hoof capsule to the underlying distal phalanx and causes loss-of-use, poor quality of life and euthanasia in horses. Historically, studies to better understand the etiology of laminitis by documenting changes in gene expression were hampered by the paucity of gene annotation specific ...
DOI:
10.1016/j.vetimm.2021.110353

59. An allergen-fused dendritic cell-binding peptide enhances in vitro proliferation of equine T-cells and cytokine production

Author:
Anja Ziegler; Judith Olzhausen; Eman Hamza; Ana Stojiljkovic; Michael H. Stoffel; Mattia Garbani; Claudio Rhyner; Eliane Marti
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110351
ISSN:
0165-2427
Subject:
Culicoides; T-lymphocytes; Toll-like receptor 4; adjuvants; agonists; allergens; asthma; cancer therapy; cell proliferation; dendritic cells; dermatitis; horses; humans; immune response; immunogenicity; immunomodulators; immunopathology; immunotherapy; insects; interleukin-10; interleukin-17; interleukin-4; mice; oligodeoxyribonucleotides; ovalbumin
Abstract:
... Allergen-specific immunotherapy (AIT) constitutes the only curative approach for allergy treatment. There is need for improvement of AIT in veterinary medicine, such as in horses suffering from insect bite hypersensitivity, an IgE-mediated dermatitis to Culicoides. Dendritic cell (DC)-targeting represents an efficient method to increase antigen immunogenicity. It is studied primarily for its use i ...
DOI:
10.1016/j.vetimm.2021.110351

60. The cathelicidin CATH-2 efficiently neutralizes LPS- and E. coli-induced activation of porcine bone marrow derived macrophages

Author:
Roel M. van Harten; Edwin J.A. Veldhuizen; Henk P. Haagsman; Maaike R. Scheenstra
Source:
Veterinary immunology and immunopathology 2022 v.244 pp. 110369
ISSN:
0165-2427
Subject:
anti-inflammatory activity; bone marrow; cathelicidins; immunomodulators; immunopathology; macrophage activation; macrophages; pathogens; risk; swine; vaccines
Abstract:
... Infectious diseases in pigs cause monetary loss to farmers and pose a zoonotic risk. Therefore, it is important to obtain more porcine specific immunological knowledge as a measure to protect against infectious diseases, for example by exploring immunomodulators that are usable as vaccine adjuvants. Cathelicidins are a class of host defence peptides (HDPs) able to directly kill microbes as well as ...
DOI:
10.1016/j.vetimm.2021.110369

61. A clonality assay in canine B cell tumors targeting the immunoglobulin light chain lambda locus

Author:
Masamine Takanosu; Yumiko Kagawa
Source:
Veterinary immunology and immunopathology 2022 v.253 pp. 110498
ISSN:
0165-2427
Subject:
B-lymphocytes; DNA; antigens; capillary electrophoresis; databases; dogs; genes; immunogenetics; immunoglobulin heavy chains; immunoglobulin light chains; immunopathology; inflammation; loci; lymph; lymphoma; polymerase chain reaction
Abstract:
... Clonality assays for antigen receptor rearrangement have been used as adjunct examinations of lymphoproliferative diseases. These assays have been useful for differentiation between inflammation and clonal expansion of lymphocytes. Whereas the immunoglobulin heavy chain (IGH) and immunoglobulin light chain kappa (IGK) loci have been targeted in canine clonality assays previously, the immunoglobuli ...
DOI:
10.1016/j.vetimm.2022.110498

62. The effect of 5-aminolevulinic acid on canine peripheral blood mononuclear cells

Author:
Masaya Igase; Nao Iwatani; Aki Sakai; Kenji Watanabe; Yoichi Mizukami; Takuya Mizuno
Source:
Veterinary immunology and immunopathology 2022 v.251 pp. 110473
ISSN:
0165-2427
Subject:
Japan; RNA; T-lymphocytes; aminolevulinic acid; concanavalin A; dogs; heme; heme oxygenase (biliverdin-producing); immunopathology; inflammation; interferon-gamma; kidneys; mitochondria; sequence analysis; sodium citrate
Abstract:
... 5-aminolevulinic acid (ALA) is a natural amino acid and a product of the first heme synthesis pathway in mitochondria. Its immunomodulatory effects have garnered recent attention for their potential application to cancer, inflammation, and autoimmune diseases in humans. A supplement containing ALA is now available in Japan to enhance ATP synthesis via mitochondrial activity. However, how ALA affec ...
DOI:
10.1016/j.vetimm.2022.110473

63. A ferret model of immunosuppression induced with dexamethasone

Author:
Anna Hundakova; Lenka Leva; Miroslav Toman; Zdenek Knotek
Source:
Veterinary immunology and immunopathology 2022 v.243 pp. 110362
ISSN:
0165-2427
Subject:
Phytolaccaceae; chemiluminescence; concanavalin A; dexamethasone; ferrets; humans; immunopathology; immunosuppression; ingestion; mitogens; monocytes; neutrophils; phagocytosis; phytohemagglutinin; reactive oxygen species
Abstract:
... Ferrets are nowadays frequently used as animal models for biomedical purposes; in many cases, immunosuppression of experimental animals is necessary. The aim of this study was to evaluate the effect of intramuscular dexamethasone administration (2 mg/kg as the initiation dose continued with 1 mg/kg q 12 h applied 5 times) on ferret’s immune system. In comparison with ferrets which received the sal ...
DOI:
10.1016/j.vetimm.2021.110362

64. The teleost polymeric Ig receptor counterpart in ballan wrasse (Labrus bergylta) differs from pIgR in higher vertebrates

Author:
Angela Etayo; Håvard Bjørgen; Erling O. Koppang; Ivar Hordvik
Source:
Veterinary immunology and immunopathology 2022 v.249 pp. 110440
ISSN:
0165-2427
Subject:
Labrus; Salmo salar; active transport; aquatic environment; bile; chemical bonding; epithelium; fish; foregut; genes; hindgut; immunologic receptors; immunopathology; kidneys; leukocytes; liquid chromatography; liver; mucosal immunity; mucus; peptides; polymers; proteinases; reverse transcriptase polymerase chain reaction; spleen
Abstract:
... As mucosal barriers in fish are the main sites where pathogens are encountered, mucosal immunity is crucial to avoid infection in the aquatic environment. In teleost fish, immunoglobulins are present in gut, gill and skin mucus, although not in the same amounts as in higher vertebrates. In mammals, the poly-Ig receptor (pIgR) is synthesized in epithelial cells and mediates the active transport of ...
DOI:
10.1016/j.vetimm.2022.110440

65. A 14 bp indel polymorphism in the promoter region is associated with different responses to porcine circovirus type 2 infection by regulating MRC1 transcription

Author:
Hao Liu; Changying Wang; Gen Liu; Yanping Li; Li Kang; Yi Sun; Yunliang Jiang
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110202
ISSN:
0165-2427
Subject:
Duroc; Porcine circovirus-2; genes; heart; immune response; immunopathology; kidneys; landraces; large intestine; liver; luciferase; lungs; lymph nodes; mannose; pathogens; promoter regions; small intestine; spleen; swine; transcription (genetics)
Abstract:
... Mannose receptor, C type 1 (MRC1) is a key factor in regulating the body's immune response to resist pathogen invasions. In this study, mRNA expressions of MRC1 gene in nine porcine organs/tissues were compared between Laiwu (LW) and Yorkshire × Landrace crossbred (YL) pigs prior to and post PCV2 infection. We found that, for pigs uninfected with PCV2, MRC1 mRNA expressions in the lung, spleen, la ...
DOI:
10.1016/j.vetimm.2021.110202

66. APB-F1, a long-acting feline granulocyte colony-stimulating factor fusion protein, created by exploiting FL335, a chimeric Fab specific for feline serum albumin

Author:
Hyun-jin Chi; Mihyun Park; Jae-kyu Han; Sun-mi Kim; SeungGoo Kang; Jin-hyuk Yang; Sang-hoon Cha
Source:
Veterinary immunology and immunopathology 2021 v.240 pp. 110322
ISSN:
0165-2427
Subject:
Cricetulus griseus; antibodies; bioactive properties; blood serum; cats; granulocyte colony-stimulating factor; half life; humans; immunopathology; neutropenia; neutrophils; pharmacodynamics; pharmacokinetics; recombinant fusion proteins; serum albumin; therapeutics
Abstract:
... Off-label use of a human granulocyte colony stimulating factor (hG-CSF) has been allowed to treat dogs and cats with neutropenia. However, repeated administration of hG-CSF induces undesirable anti-drug antibody (ADA) responses, implying the necessity of animal-derived G-CSF as a therapeutic reagent, preferably with a long-acting capability. Herein, we generated a recombinant fusion protein by gen ...
DOI:
10.1016/j.vetimm.2021.110322

67. Activation of canine neutrophils by platelet-activating factor

Author:
Ken Okabayashi; Shuichiro Kanai; Fumihiko Katakura; Riku Takeuchi; Takashi Yamauchi; Shunya Nakayama; Rie Kinoshita; Hiroshi Koie; Takanori Narita
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110336
ISSN:
0165-2427
Subject:
calcium; dogs; endoplasmic reticulum; immunopathology; inflammation; inositols; neutrophils; phagocytosis; platelet aggregation; platelet-activating factor; respiratory burst; signal transduction; suramin
Abstract:
... Neutrophils are essential for innate immunity as the first line of defence. Neutrophils act as phagocytic white blood cells to kill bacteria and other microorganisms. A strong respiratory burst of neutrophils, dependent on reactive oxygen species, is produced during phagocytosis. Platelet-activating factor (PAF) is a signalling molecule with several prominent roles in tissue injury, inflammation, ...
DOI:
10.1016/j.vetimm.2021.110336

68. Activation of chicken gamma-delta T lymphocytes by a purified ulvan extract

Author:
Nathalie Guriec; Frédérick Bussy; Christelle Gouin; Olivier Mathiaud; Matthieu Le Goff; Jacques Delarue; Pi Nyvall Collén
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110255
ISSN:
0165-2427
Subject:
Avian orthoavulavirus 1; Salmonella; Ulva; chickens; cytotoxicity; flow cytometry; heterophils; immune response; immunopathology; intestines; major histocompatibility complex; monocytes; polysaccharides; spleen; vaccination
Abstract:
... Chicken γδ T lymphocytes are present in a variety of tissues such as blood, spleen and intestine. They constitute a major cytotoxic population. In chicken, Salmonella immunization as well as vaccination against Newcastle disease virus are accompanied by an increase of γδ T lymphocytes in peripheral blood, which may be activated, and thus represent a protective immune response. It has been publishe ...
DOI:
10.1016/j.vetimm.2021.110255

69. Alterations in TNF-α and its receptors expression in cows undergoing heat stress

Author:
Pamela Anahí Lendez; Lucía Martinez Cuesta; Maria Victoria Nieto Farias; Adrian Alejandro Vater; Marcelo Daniel Ghezzi; Daniel Mota-Rojas; Guillermina Laura Dolcini; María Carolina Ceriani
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110232
ISSN:
0165-2427
Subject:
animal welfare; autumn; equations; heat stress; humidity; immune response; immunopathology; milk industry; reproductive performance; spring; summer; temperature
Abstract:
... Heat stress is one of the environmental factors that most severely affects milk industry, as it has impact on production, immune responses and reproductive performance. The present study was conducted with high-performance Holando-Argentino cows. Our objective was to study TNF-α and its receptors pattern expression in cows from a region characterized by extreme climatic seasonality. Animals were e ...
DOI:
10.1016/j.vetimm.2021.110232

70. Analysis of canine myeloid-derived suppressor cells (MDSCs) utilizing fluorescence-activated cell sorting, RNA protection mediums to yield quality RNA for single-cell RNA sequencing

Author:
K. Jackson; R.J. Milner; A. Doty; S. Hutchison; G. Cortes-Hinojosa; A. Riva; B. Sahay; A. Lejeune; S. Bechtel
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110144
ISSN:
0165-2427
Subject:
GATA transcription factors; RNA; antibodies; cell viability; dogs; flow cytometry; fluorescence; genomics; immunopathology; magnetism; melanoma; ribonucleases; transcription (genetics)
Abstract:
... Fluorescence-activated cell sorting (FACS) is a branch of flow cytometry that allows for the isolation of specific cell populations that can then be further analyzed by single-cell RNA sequencing (scRNA-seq). When utilizing FACS for population isolation prior to sequencing, it is essential to consider the protection of RNA from RNase activity, environmental conditions, and the sorting efficiency t ...
DOI:
10.1016/j.vetimm.2020.110144

71. Anti-inflammatory activity of citrus pectin on chicken monocytes' immune response

Author:
G. Ávila; D. De Leonardis; G. Grilli; C. Lecchi; F. Ceciliani
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110269
ISSN:
0165-2427
Subject:
Citrus; Escherichia coli; anti-inflammatory activity; apoptosis; biochemical pathways; caspase-3; chemotaxis; dietary fiber; digestibility; dose response; energy; galacturonic acid; immune response; immunopathology; monocytes; pectins; phagocytosis; toxicity testing; viability
Abstract:
... Pectin is a dietary fibre composed of galacturonic acid, primarily found in the citrus fruits' cell walls. Citrus pectin (CP) has demonstrated antioxidative, anticancer, and anti-inflammatory properties in humans and animals. In broilers, CP supplementation improves energy utilization and nutrient digestibility, but limited information on its effects on chicken immunity is available so far. This s ...
DOI:
10.1016/j.vetimm.2021.110269

72. Assessment of three commercial ELISAs for the detection of antibodies against Porcine epidemic diarrhea virus at different stages of the immune response

Author:
I. Díaz; J. Pujols; E. Cano; R. Cuadrado; N. Navarro; E. Mateu; M. Martín
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110206
ISSN:
0165-2427
Subject:
Porcine epidemic diarrhea virus; immune response; immunopathology; nucleocapsid proteins
Abstract:
... Three commercial ELISAs -two based on spike (E1 and E3) and one on nucleocapsid protein (E2)-were used to analyze the development and persistence of antibodies against Porcine epidemic diarrhea virus (PEDV). Seventy-five four-week-old PEDV-negative piglets were inoculated orally with a European G1b PEDV (INOC) and fourteen were kept as controls (CTRL). After the inoculation, E3 detected positive a ...
DOI:
10.1016/j.vetimm.2021.110206

73. Association of metabolic markers with neutrophil function in healthy postpartum dairy cows

Author:
O. Bogado Pascottini; T.C. Bruinjé; R. Couto Serrenho; B. Mion; S.J. LeBlanc
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110182
ISSN:
0165-2427
Subject:
Holstein; albumins; aspartate transaminase; blood serum; body condition; calcium; cholesterol; energy; flow cytometry; fluorescence; gamma-glutamyltransferase; globulins; glucose; glutamate dehydrogenase; haptoglobins; immunopathology; inflammation; insulin-like growth factor I; liver; magnesium; neutrophils; phagocytosis; phosphorus; protein content; proteolysis; regression analysis; urea; variance
Abstract:
... The objective of this study was to evaluate the associations of serum markers for systemic inflammation, liver, mineral, and energy status, and blood neutrophil counts with the function of circulating neutrophils in postpartum dairy cows. Blood samples were collected from 21 healthy Holstein cows at 5, 10, 14, and 21 d postpartum. Serum samples were used to measure concentrations of total calcium, ...
DOI:
10.1016/j.vetimm.2020.110182

74. Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate

Author:
Matthew J. Cascio; Elizabeth M. Whitley; Bikash Sahay; Galaxia Cortes-Hinojosa; Lung-Ji Chang; Jonathan Cowart; Marc Salute; Elias Sayour; Michael Dark; Zachary Sandoval; Duane A. Mitchell; Rowan J. Milner
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110169
ISSN:
0165-2427
Subject:
T-lymphocytes; dogs; humans; immunohistochemistry; immunopathology; ligands; metastasis; osteosarcoma; patients
Abstract:
... Immune-targeted therapies are being successfully implemented into cancer clinical practice. In particular checkpoint inhibitors are employed to modulate the immune microenvironment of solid tumors. We sought to determine the expression of PD-L1, HVEM, and B7H3 in human and canine osteosarcoma, and correlate expression with clinical features and tumor infiltrating lymphocytes in naturally-occurring ...
DOI:
10.1016/j.vetimm.2020.110169

75. Characterization of a variant CD4 molecule in Japanese Black cattle

Author:
Yuko Kato-Mori; Taku Okamura; Norihito Kawashita; Katsuro Hagiwara
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110167
ISSN:
0165-2427
Subject:
Wagyu; amino acid substitution; amino acids; cytokines; epitopes; farms; flow cytometry; gene expression; genes; glycoproteins; immunopathology; macrophages; mitogens; monocytes
Abstract:
... Monoclonal antibodies (mAbs) that recognize cluster of differentiation (CD) molecules on lymphocytes are useful tools for the study of different lymphocyte subsets in flow cytometry (FCM) analysis. CD4 is a glycoprotein found on the surfaces of helper T cells, monocytes, macrophages, and dendritic cells. In this study, we describe Japanese Black (JB) calves in a farm whose peripheral blood mononuc ...
DOI:
10.1016/j.vetimm.2020.110167

76. Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth

Author:
E. Maurizio; M.D. Trangoni; U.A. Rossi; M. Dunleavy; C. Colato; C.A. Rossetti
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110223
ISSN:
0165-2427
Subject:
Brucella melitensis; brucellosis; cattle; cell-mediated immunity; immunopathology; innate immunity; keyhole limpet hemocyanin; macrophages; monocytes; nitrogen; oxygen; pathogens; phenotype; zoonoses
Abstract:
... Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exh ...
DOI:
10.1016/j.vetimm.2021.110223

77. Clinical and immunological responses in sheep after inoculation with Himar1-transformed Anaplasma phagocytophilum and subsequent challenge with a virulent strain of the bacterium

Author:
Sveinung Eskeland; Snorre Stuen; Ulrike G. Munderloh; Anthony Barbet; Liliana Crosby; Kari Lybeck; Peter Wilhelmsson; Per-Eric Lindgren; Shokouh Makvandi-Nejad; Stig Tollefsen; Erik G. Granquist
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110165
ISSN:
0165-2427
Subject:
Anaplasma phagocytophilum; acaricides; animal welfare; bacteremia; bacteria; cell lines; disease control; genetic transformation; humoral immunity; immunogenicity; immunopathology; nucleotide sequences; tick-borne diseases; ticks; vaccines; virulence; virulent strains; Norway
Abstract:
... In Norway, the tick-transmitted bacterium Anaplasma phagocytophilum is estimated to cause tick-borne fever (TBF) in 300 000 lambs on pastures each year, resulting in economic and animal welfare consequences. Today, prophylactic measures mainly involve the use of acaricides, but a vaccine has been requested by farmers and veterinarians for decades. Several attempts have been made to produce a vacci ...
DOI:
10.1016/j.vetimm.2020.110165

78. Clostridium butyricum CB1 up-regulates FcRn expression via activation of TLR2/4-NF-κB signaling pathway in porcine small intestinal cells

Author:
Chenxi Li; Rui Cao; Shaoju Qian; Chenyuan Qiao; Xi Liu; Zutao Zhou; Zili Li
Source:
Veterinary immunology and immunopathology 2021 v.240 pp. 110317
ISSN:
0165-2427
Subject:
Clostridium butyricum; blood serum; epithelium; heat; humoral immunity; immunoglobulin G; immunopathology; intestines; mucosal immunity; probiotics; swine; transcription factor NF-kappa B
Abstract:
... The neonatal Fc receptor (FcRn) mediates the bidirectional transport of immunoglobulin G (IgG) across hyperpolarized epithelial cells. Overexpression of FcRn increases serum IgG and humoral immune response. Probiotics can improve the host’s serum and intestinal mucosal IgG. However, whether probiotics regulate FcRn and its specific mechanism are still unclear. Our research showed that heat inactiv ...
DOI:
10.1016/j.vetimm.2021.110317

79. Comparative cellular immune responses in calves after infection with Mycobacterium avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis

Author:
Stabel J.R.; W.R. Waters; J.P. Bannantine; M.V. Palmer
Source:
Veterinary immunology and immunopathology 2021 v.237 no. pp. 110268
ISSN:
0165-2427
Subject:
Mycobacterium avium subsp. paratuberculosis; Mycobacterium bovis; Mycobacterium kansasii; antigens; cell-mediated immunity; immunopathology; interferon-gamma; interleukin-10; interleukin-12; secretion
Abstract:
... In the present study, calves were infected with Mycobacterium avium subsp. paratuberculosis (MAP), Mycobacterium avium subsp. avium (M. avium), Mycobacterium kansasii (M. kansasii), or Mycobacterium bovis (M. bovis) to determine differences in cellular immunity. Comparative cellular responses were assessed upon stimulation of cells with mycobacterial whole cell sonicates respective of each infecti ...
Handle:
10113/7377001
DOI:
10.1016/j.vetimm.2021.110268
CHORUS:
10.1016/j.vetimm.2021.110268

80. Comparative profiling of the resistance of different genotypes of mannose-binding lectin to Mycoplasma pneumoniae infection in Chinese Merino sheep based on high-throughput sequencing technology

Author:
Mengting Zhu; Ying Nan; Mengting Zhai; Mingyuan Wang; Yanyan Shao; Hugh T. Blair; Stephen Todd Morris; Paul Richard Kenyon; Zongsheng Zhao; Hongmei Zhang
Source:
Veterinary immunology and immunopathology 2021 v.233 pp. 110183
ISSN:
0165-2427
Subject:
GATA transcription factors; Merino; Mycoplasma pneumoniae; blood; gene expression; gene expression regulation; glycoproteins; immunopathology; immunotherapy; innate immunity; lectins; liver; microRNA; sheep
Abstract:
... Mannose-binding lectin (MBL) glycoproteins in blood can selectively recognise lectins on the surface of bacteria, and play an important role in natural immunity. Micro RNAs (miRNAs) are key molecules that regulate gene expression at the post-transcriptional level in vivo, and their pathways are specific and effective. Previous studies indicate that small RNAs such as miRNAs perform regulatory role ...
DOI:
10.1016/j.vetimm.2021.110183

81. Comparison of cellular immune responses to avian influenza virus in two genetically distinct, highly inbred chicken lines

Author:
Emily J. Aston; Ying Wang; Karen E. Tracy; Rodrigo A. Gallardo; Susan J. Lamont; Huaijun Zhou
Source:
Veterinary immunology and immunopathology 2021 v.235 pp. 110233
ISSN:
0165-2427
Subject:
B-lymphocytes; Influenza A virus; T-lymphocytes; antibodies; avian influenza; blood serum; chickens; eggs; flow cytometry; gastrointestinal system; immunopathology; macrophages; nose; pathogenicity; vaccination; viral load; viruses
Abstract:
... Low pathogenicity avian influenza causes mild disease involving the respiratory, gastrointestinal, and reproductive systems of wild and domestic birds. Avian influenza research often emphasizes the effect of the virus genetics on disease, but the influence of host genetics on resistance to infection is not well understood. The genetic determinants of enhanced resistance to influenza can be explore ...
DOI:
10.1016/j.vetimm.2021.110233

82. Comparison of recombinant Culicoides allergens produced in different expression systems for IgE serology of insect bite hypersensitivity in horses of different origins

Author:
Sigridur Jonsdottir; Sigurbjorg Torsteinsdottir; Vilhjalmur Svansson; Johannes Gudbrandsson; Sara Bjork Stefansdottir; Jon Mar Bjornsson; Arna Runarsdottir; Eliane Marti
Source:
Veterinary immunology and immunopathology 2021 v.238 pp. 110289
ISSN:
0165-2427
Subject:
Culicoides; Escherichia coli; barley; dermatitis; hypersensitivity; immunopathology; serology; Iceland
Abstract:
... Insect bite hypersensitivity (IBH) is an allergic dermatitis of horses, an IgE-mediated reaction to Culicoides midges. Causative Culicoides spp. are not indigenous in Iceland resulting in high prevalence of IBH in horses born in Iceland and exported as compared to Icelandic horses born in a Culicoides rich environment. The aims were (i) to compare IgE levels in sera of IBH-affected horses born in ...
DOI:
10.1016/j.vetimm.2021.110289

83. Comparison of the immune response following subcutaneous versus intranasal modified-live virus booster vaccination against bovine respiratory disease in pre-weaning beef calves that had received primary vaccination by the intranasal route

Author:
Roberto A. Palomares; João H.J. Bittar; Amelia R. Woolums; Alejandro Hoyos-Jaramillo; David J. Hurley; Jeremiah T. Saliki; Maria S. Ferrer; Anna C. Bullington; Adriana Rodriguez; Tyler Murray; Merrilee Thoresen; Katie Jones; Agne Stoskute
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110254
ISSN:
0165-2427
Subject:
beef; blood serum; bovine respiratory disease; cytokines; gene expression; immune response; immunopathology; mucosal immunity; nose; secondary immunization; secretion; statistical analysis; vaccination; vaccines; viruses
Abstract:
... This study was performed to elucidate whether the route of booster vaccination affects the immune response against respiratory vaccine viruses in pre-weaning beef calves that receive primary intranasal (IN) vaccination during the first month of life. The objective was to compare the serum neutralizing antibody (SNA) titers to BHV1, BRSV, and BPI3V, cytokine mRNA expression and mucosal BHV1- and BR ...
DOI:
10.1016/j.vetimm.2021.110254

84. Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens

Author:
Huipeng Lu; Xiaohui Zhou; Zhi Wu; Xinyu Zhang; Liqi Zhu; Xiaoyu Guo; Quan Zhang; Shanyuan Zhu; Hongfei Zhu; Huaichang Sun
Source:
Veterinary immunology and immunopathology 2021 v.239 pp. 110307
ISSN:
0165-2427
Subject:
Adenoviridae; African swine fever virus; Mycobacterium tuberculosis; Toll-like receptors; Vibrio; antibodies; antibody formation; antigens; flagellin; heat-shock protein 70; immunopathology; interleukin-10; interleukin-4; ligands; lungs; macrophages; mucosal immunity; nose; secondary immunization; swine; vaccination; vaccine adjuvants; vaccine development
Abstract:
... The mucosal immunity plays an important role against African swine fever virus (ASFV) infection and the efficacy of mucosal vaccination is highly dependent on the adjuvant. However, the mucosal adjuvant for ASFV vaccination is poorly studied. Toll-like receptor (TLR) ligands such as the FlaB flagellin from Vibrio vulnificus and the heat shock protein 70 from Mycobacterium tuberculosis (mHsp70) hold ...
DOI:
10.1016/j.vetimm.2021.110307

85. Comparison of the response of mammary gland tissue from two divergent lines of goat with high and low milk somatic cell scores to an experimental Staphylococcus aureus infection

Author:
Rossana Capoferri; Paola Cremonesi; Bianca Castiglioni; Giuliano Pisoni; Paola Roccabianca; Federica Riva; Joel Filipe; Marcello del Corvo; Alessandra Stella; John L. Williams; Rachel Rupp; Paolo Moroni
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110208
ISSN:
0165-2427
Subject:
Staphylococcus aureus; chemotaxis; goats; histology; histopathology; immune response; immunopathology; inflammation; interleukin-6; livestock and meat industry; mammary glands; milk; milk quality; neutrophils; somatic cells; transcriptomics; udders
Abstract:
... Mastitis represents one of the major economic and health threats to the livestock sector associated with reduction in milk quality, loss of production and is a major reason for culling. Somatic cell score (SCS) is used as a criterion in breeding programmes to select cows genetically less susceptible to mastitis. The relevance of SCS as a predictor of udder health and susceptibility to mastitis is ...
DOI:
10.1016/j.vetimm.2021.110208

86. Continuous digital hypothermia reduces expression of keratin 17 and 1L-17A inflammatory pathway mediators in equine laminitis induced by hyperinsulinemia

Author:
Lynne Cassimeris; Caitlin Armstrong; Quinnlyn C. Burger; Simon Stokes; Andrew van Eps; Hannah Galantino-Homer
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110326
ISSN:
0165-2427
Subject:
ambient temperature; fluorescent antibody technique; horses; human diseases; hyperinsulinemia; hypothermia; immunopathology; inflammation; interleukin-17; interleukin-6; keratin; keratinocytes; laminitis; mice
Abstract:
... The euglycemic hyperinsulinemic clamp model (EHC) of equine endocrinopathic laminitis induces rapid loss of lamellar tissue integrity, disrupts keratinocyte functions, and induces inflammation similar to natural disease. Continuous digital hypothermia (CDH) blocks tissue damage in this experimental model, allowing identification of specific genes or molecular pathways contributing to disease initi ...
DOI:
10.1016/j.vetimm.2021.110326

87. Cytokine biomarker discovery in the white rhinoceros (Ceratotherium simum)

Author:
Josephine Chileshe; Tanya J. Kerr; Craig Kinnear; Peter E. Buss; Paul D. van Helden; Robin M. Warren; Michele A. Miller; Sven D.C. Parsons
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110168
ISSN:
0165-2427
Subject:
Ceratotherium simum; Mycobacterium bovis; RNA; antigens; biomarkers; blood; bovine tuberculosis; gene expression; horses; immunopathology; interferon-gamma; threatened species
Abstract:
... Bovine tuberculosis (bTB), caused by Mycobacterium bovis (M. bovis) infection, disrupts conservation programs of threatened species such as the white rhinoceros (Ceratotherium simum). Interferon gamma release assays have been developed for the diagnosis of M. bovis infection in rhinoceros, however, the discovery of additional diagnostic biomarkers might improve the accuracy of case detection. The ...
DOI:
10.1016/j.vetimm.2020.110168

88. Cytokine expression in the eye and brain of chickens following infection with a very virulent plus Marek’s disease virus strain

Author:
Coral Salvador; Aneg L. Cortes; Arun R. Pandiri; Isabel M. Gimeno
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110277
ISSN:
0165-2427
Subject:
brain; chickens; eyes; immunopathology; interleukin-15; interleukin-18; interleukin-8; nervous system diseases; paralysis; pathogenesis; virulence
Abstract:
... Cytokine transcripts were evaluated chronologically in the brain and in the eye of chickens infected with the very virulent plus Marek’s disease virus (vv + MDV) strain 648A. Brain and eye samples were collected from chickens that were either suffering from transient paralysis (TP) (11 days post inoculation, dpi) or had completely recovered from TP but started developing clinical signs of persiste ...
DOI:
10.1016/j.vetimm.2021.110277

89. Cytokine profile and parasite load in lymph nodes of dogs naturally infected with Leishmania infantum from distinct epidemiological scenarios in São Paulo State, Brazil

Author:
Juliana M. Guerra; Natália C.C.A. Fernandes; Rodrigo A. Réssio; Lidia M. Kimura; José E.R. Barbosa; Helena H. Taniguchi; Roberto M. Hiramoto; Gabriela Motoie; José E. Tolezano; Bruno Cogliati
Source:
Veterinary immunology and immunopathology 2021 v.233 pp. 110198
ISSN:
0165-2427
Subject:
Leishmania infantum; babesiosis; chemotypes; disease progression; ehrlichiosis; histiocytosis; histopathology; immune response; immunohistochemistry; immunopathology; interleukin-4; lymph; lymph nodes; parasite load; parasites; visceral leishmaniasis; Brazil
Abstract:
... Visceral leishmaniasis (VL) is an important zoonotic vector-borne disease and domestic dogs are considered the main domiciliary and peri-domiciliary reservoir of Leishmania (Leishmania) infantum in South America. Distinct eco-epidemiological scenarios associated to the prevalence of the disease, clusters of parasite genotypes and chemotypes of vectors population are described in Brazil, especially ...
DOI:
10.1016/j.vetimm.2021.110198

90. Detection of bovine inflammatory cytokines IL-1β, IL-6, and TNF-α with a multiplex electrochemiluminescent assay platform

Author:
Chitko Carol; Carol G. Chitko-McKown; Stacy L. Bierman; Larry A. Kuehn; Gary L. Bennett; Keith D. DeDonder; Michael D. Apley; Gregory P. Harhay; Michael L. Clawson; Bradley J. White; Robert L. Larson; Sarah F. Capik; Brian V. Lubbers
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110274
ISSN:
0165-2427
Subject:
cattle; immunopathology; interleukin-6
Abstract:
... Commercially available bovine-specific assays are limited in number, and multiplex assays for this species are rare. Our objective was to develop a multiplex assay for the bovine inflammatory cytokines IL-1β, IL-6, and TNF-α using the Meso Scale Discovery U-PLEX platform. "Do-It-Yourself" ELISA kits that contained polyclonal antibodies, both unlabeled and biotinylated, and the specific recombinant ...
Handle:
10113/7391751
DOI:
10.1016/j.vetimm.2021.110274
CHORUS:
10.1016/j.vetimm.2021.110274

91. Detection of osteoarthritis in dogs by metabolic, pro-inflammatory and degenerative synovial fluid biomarkers and traditional radiographic screening: A pilot study

Author:
E. de Bakker; B. Broeckx; K. Demeyere; V. Stroobants; B. Van Ryssen; E. Meyer
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110252
ISSN:
0165-2427
Subject:
biomarkers; cartilage; early diagnosis; gelatinase A; glucose; immunopathology; interleukin-1beta; lactic acid; osteoarthritis; pH; radiography; synovial fluid; tumor necrosis factor-alpha
Abstract:
... Secondary osteoarthritis (OA) is a slow progressive, common disorder of synovial joints in dogs. It is characterized by a loss of balance between the synthesis and degeneration of articular cartilage components. Its diagnosis is currently based on the presence of clear radiographic changes, which only occur in the later stages of the disease. Hence, early diagnosis of OA remains a major problem. T ...
DOI:
10.1016/j.vetimm.2021.110252

92. Development of A recombinant nucleocapsid based indirect ELISA for the detection of antibodies to avian metapneumovirus subtypes, A, B, and C

Author:
Wanhong Xu; Matthew Suderman; Janice Koziuk; Davor Ojkic; Yohannes Berhane
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110151
ISSN:
0165-2427
Subject:
Avian metapneumovirus; Trichoplusia ni; antibodies; antibody formation; antigens; blood serum; chickens; fluorescence; immunopathology; insects; monitoring; nucleocapsid; serodiagnosis
Abstract:
... Nucleocapsid (N) protein is the most highly expressed of all avian metapneumovirus (aMPV) viral proteins and stimulates a substantial immune response in infected animals. Codon optimized recombinant N (rec-N) protein from aMPV subtypes A, B, and C were expressed using the baculoviral expression system in Trichoplusia ni (Tni) insect cells. A mixture of purified rec-N antigens from each subtype was ...
DOI:
10.1016/j.vetimm.2020.110151

93. Development of a cell line-based assay to measure the antibody-dependent cellular cytotoxicity of a canine therapeutic antibody

Author:
Takuya Mizuno; Yoshiho Takeda; Toshihiro Tsukui; Masaya Igase
Source:
Veterinary immunology and immunopathology 2021 v.240 pp. 110315
ISSN:
0165-2427
Subject:
antibodies; cancer therapy; cytotoxicity; dogs; humans; immunopathology; natural killer cells
Abstract:
... Antibody drugs are one of the most important therapeutic modalities in cancer treatment. Antibody drugs for canine cytotoxicity have been developed, and the most important way to evaluate the function of antibody drugs in vitro is to measure the antibody-dependent cellular cytotoxicity (ADCC) activity. However, a good method for measuring the ADCC activity of antibody drugs for dogs has not yet be ...
DOI:
10.1016/j.vetimm.2021.110315

94. Differential gene expression and Ingenuity Pathway Analysis of bronchoalveolar lavage cells from horses with mild/moderate neutrophilic or mastocytic inflammation on BAL cytology

Author:
Kaori Uchiumi Davis; M. Katie Sheats
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110195
ISSN:
0165-2427
Subject:
asthma; cell biology; chemokines; descriptive studies; disease severity; etiology; fibrosis; gene expression; gene expression regulation; horses; immunoassays; immunopathology; inflammation; pathophysiology; sequence analysis; transcriptome
Abstract:
... Mild to moderate equine asthma syndrome (mEAS) affects horses of all ages and breeds. To date, the etiology and pathophysiology of mEAS are active areas of research, and it remains incompletely understood whether mEAS horses with different immune cell ‘signatures’ on BAL cytology represent different phenotypes, distinct pathobiological mechanisms (endotypes), varied environmental conditions, disea ...
DOI:
10.1016/j.vetimm.2021.110195

95. Do allogeneic bone marrow derived mesenchymal stem cells diminish the inflammatory response to lipopolysaccharide infusion in horses? A pilot study

Author:
Isabelle Kilcoyne; Jorge E. Nieto; Johanna L. Watson; Larry D. Galuppo; Dori L. Borjesson
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110146
ISSN:
0165-2427
Subject:
Escherichia coli; bone marrow; colic; cytokines; endotoxemia; horses; immunopathology; industry; inflammation; intravenous injection; lipopolysaccharides; morbidity; mortality
Abstract:
... Endotoxemia is a leading cause of morbidity and mortality in the equine industry, with colic being the most common cause of endotoxemia in horses. The objective of this study was to evaluate the safety and potential efficacy of a single dose of allogeneic equine bone marrow derived mesenchymal stem cells (BM-MSCs) in horses after the IV administration of lipopolysaccharide (LPS).Six horses were ad ...
DOI:
10.1016/j.vetimm.2020.110146

96. Dog leukocyte antigen (DLA) class II genotypes associated with chronic enteropathy in French bulldogs and miniature dachshunds

Author:
Meg Nakazawa; Jiro Miyamae; Masaharu Okano; Hideyuki Kanemoto; Fumihiko Katakura; Takashi Shiina; Koichi Ohno; Hajime Tsujimoto; Tadaaki Moritomo; Toshihiro Watari
Source:
Veterinary immunology and immunopathology 2021 v.237 pp. 110271
ISSN:
0165-2427
Subject:
Dachshund; German Shepherd; alleles; antigens; digestive system diseases; disease prevention; dogs; early diagnosis; epigenetics; gastrointestinal system; genotyping; haplotypes; hepatitis; immunogenetics; immunopathology; inflammation; leukocytes; phenotype; risk
Abstract:
... Canine chronic enteropathy (CE) is a group of immunogenetic disorders of unclear etiology characterized by chronic or recurrent gastrointestinal signs and inflammation. Diagnosis of CE subtypes by treatment response is a lengthy and challenging process, particularly in refractory cases of the disease. Given known association of dog leukocyte antigen (DLA) class II genotype and various immunogeneti ...
DOI:
10.1016/j.vetimm.2021.110271

97. Dynamics of local gene regulations in synovial fluid leukocytes from horses with lipopolysaccharide-induced arthritis

Author:
Marie Walters; Kerstin Skovgaard; Pia Haubro Andersen; Peter M.H. Heegaard; Stine Jacobsen
Source:
Veterinary immunology and immunopathology 2021 v.241 pp. 110325
ISSN:
0165-2427
Subject:
antagonists; arthritis; cartilage; chemokines; chondrocytes; gene expression; genes; immunopathology; inflammation; interleukin-10; interleukin-6; lipopolysaccharides; models; neutrophil collagenase; prostaglandin synthase; superoxide dismutase; synovial fluid; tumor necrosis factors
Abstract:
... The role of resident cells such a synoviocytes and chondrocytes in intra-articular inflammation is well-characterized, however the in vivo gene expression patterns of cells (predominantly leukocytes) in the synovial fluid (SF) of an inflamed joint have never previously been investigated. The aim of this study was to investigate gene expression in SF leukocytes from the inflamed joint cavity after ...
DOI:
10.1016/j.vetimm.2021.110325

98. Dysregulation of gastrointestinal RAGE (receptor for advanced glycation end products) expression in dogs with chronic inflammatory enteropathy

Author:
A.I. Cabrera-García; M. Protschka; G. Alber; S. Kather; F. Dengler; U. Müller; J.M. Steiner; R.M. Heilmann
Source:
Veterinary immunology and immunopathology 2021 v.234 pp. 110216
ISSN:
0165-2427
Subject:
C-reactive protein; biomarkers; blood serum; colon; digestive system diseases; digestive tract; dogs; duodenum; electrophoresis; epithelium; histology; ileum; immunoassays; immunopathology; inflammation; ligands; pathogenesis; patients; protein depletion; stomach
Abstract:
... The pathogenesis of chronic inflammatory enteropathies (CIE) in dogs involves dysregulated innate immune responses. The receptor for advanced glycation end products (RAGE), a pattern recognition receptor, plays a role in chronic inflammation. Abrogation of proinflammatory RAGE signaling by ligand binding (e.g., S100/calgranulins) to soluble RAGE (sRAGE) might also be a novel therapeutic avenue. Se ...
DOI:
10.1016/j.vetimm.2021.110216

99. Ectodomain shedding by ADAM17 (a disintegrin and metalloproteinase 17) in canine neutrophils

Author:
Kristin M. Snyder; Camille A. McAloney; Joshua S. Montel; Jaime F. Modiano; Bruce Walcheck
Source:
Veterinary immunology and immunopathology 2021 v.231 pp. 110162
ISSN:
0165-2427
Subject:
adhesion; dogs; humans; immunopathology; inflammation; metalloproteinases; neutrophils; therapeutics
Abstract:
... ADAM17 is a transmembrane protease expressed by most cells in humans and mice that cleaves cell surface substrates primarily in a cis manner, a process referred to as ectodomain shedding. ADAM17 has numerous substrates and plays a broad role in various physiological processes, including as a key regulator of inflammation. At this time, little is known about ADAM17 expression and function in dogs. ...
DOI:
10.1016/j.vetimm.2020.110162

100. Effect of IL-8 haplotype on temporal profile in circulating concentrations of interleukin 8 and 25(OH) vitamin D in Holstein-Friesian calves

Author:
Megan B. O’Brien; Rachel M. McLoughlin; Colm Roche; Corwin D. Nelson; Kieran G. Meade
Source:
Veterinary immunology and immunopathology 2021 v.238 pp. 110287
ISSN:
0165-2427
Subject:
Holstein; blood serum; disease susceptibility; genes; haplotypes; immune response; immunopathology; innate immunity; interleukin-8; monocytes; neutrophils; temporal variation; vitamin D
Abstract:
... Interleukin-8 (IL-8) is an inflammatory chemokine released during the primary innate immune response to recruit neutrophils to the site of infection. Two distinct gene promoter haplotypes have been previously reported to segregate in the Holstein-Friesian breed (IL8-h1 and IL8-h2). Our earlier work showed how these divergent IL8 haplotypes influence IL-8 concentration and other immune response par ...
DOI:
10.1016/j.vetimm.2021.110287