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1. A comparative study of utilization of single and mixed phenolic compounds by individual and mixed culture

Author:
Tyagaturu Renukaprasad, Monisha; Ismailsab, Mukram; Reddy, Pooja V.; Baburao, Kirankumar; Nayak, Anand S.; Karegoudar, T. B.
Source:
3 Biotech 2017 v.7 no.3 pp. 215
ISSN:
2190-572X
Subject:
Pseudomonas; Stenotrophomonas; Xanthomonas; agricultural wastes; caffeic acid; carbon; energy; enrichment culture; mixed culture
Abstract:
... Three bacterial strains; Pseudomonas sp. TRMK1, Stenotrophomonas sp. TRMK2 and Xanthomonas sp. TRMK3 were isolated from agro-industrial waste by enrichment culture technique that are capable of utilizing phenolic acids as sole source of carbon and energy. These strains were found to utilize p-coumaric, ferulic and caffeic acid. The individual strains utilized 5 mM of mixed phenolic acids within 20 ...
DOI:
10.1007/s13205-017-0815-5
PubMed:
28669074
PubMed Central:
PMC5494029

2. Addition of Rubber to soil damages the functional diversity of soil

Author:
Goswami, Madhurankhi; Bhattacharyya, Purnita; Tribedi, Prosun
Source:
3 Biotech 2017 v.7 no.3 pp. 173
ISSN:
2190-572X
Subject:
color; ecosystems; enzyme activity; fluorescein diacetate; functional diversity; gloves; hydrolysis; isoprene; landfills; microbial activity; multivariate analysis; polymers; rubber; soil; soil ecology; soil sampling
Abstract:
... Rubber is a polymer of isoprene, consisting mainly of cis-1,4-polyisoprene units. The unmanageable production and its irresponsible disposal pose severe threats to environmental ecology. Therefore, the current study focuses extensively on the ill-effects of Rubber disposal on soil microbial functional diversity as it reflects the health of ecosystem by acting as a key component in ecosystem produc ...
DOI:
10.1007/s13205-017-0854-y
PubMed:
28660461
PubMed Central:
PMC5489457

3. Analysis of transcription factors among differentially expressed genes induced by drought stress in Populus davidiana

Author:
Mun, Bong-Gyu; Lee, Sang-Uk; Park, Eung-Jun; Kim, Hyun-Ho; Hussain, Adil; Imran, Qari Muhammad; Lee, In-Jung; Yun, Byung-Wook
Source:
3 Biotech 2017 v.7 no.3 pp. 209
ISSN:
2190-572X
Subject:
Populus; drought; forest trees; genes; nucleotide sequences; plant response; polyethylene glycol; protein structure; quantitative polymerase chain reaction; sequence analysis; stress response; transcription (genetics); transcription factors; transcriptomics; water stress; Korean Peninsula
Abstract:
... Populus davidiana is native to the Korean Peninsula and is one of the most dominant and abundantly growing forest trees in eastern Asia. Compared to other Populus species such as P. trichocarpa, P. euphratica, and P. tremula, relatively little is known about P. davidiana. Here, we performed transcriptomic analysis of P. davidiana under drought stress induced by 10% polyethylene glycol. A total of ...
DOI:
10.1007/s13205-017-0858-7
PubMed:
28667649
PubMed Central:
PMC5493580

4. An efficient process for the transformation of betulin to betulinic acid by a strain of Bacillus megaterium

Author:
Kumar, Dhirendra; Dubey, Kashyap Kumar
Source:
3 Biotech 2017 v.7 no.3 pp. 157
ISSN:
2190-572X
Subject:
Bacillus megaterium; antineoplastic activity; antiretroviral agents; betulin; betulinic acid; biomass; biotransformation; culture flasks; fermenters; moieties; pH; selective media; statistical analysis; temperature
Abstract:
... Betulinic acid as a derivative of betulin is widely reported for its anti-HIV and antitumor activities. Betulin has three most significant positions, i.e., primary hydroxyl group at position C-28, secondary hydroxyl group at position C-3, and alkene moiety at position C-20, where chemical modifications were performed to yield pharmacologically more active derivatives. Bioconversion optimization wa ...
DOI:
10.1007/s13205-017-0759-9
PubMed:
28623491
PubMed Central:
PMC5473782

5. An improved method suitable for isolation of high-quality metagenomic DNA from diverse soils

Author:
Verma, Sumit Kumar; Singh, Himanshi; Sharma, Prakash C.
Source:
3 Biotech 2017 v.7 no.3 pp. 171
ISSN:
2190-572X
Subject:
DNA; calcium chloride; cellulosic wastes; cetyltrimethylammonium bromide; edaphic factors; gardens; genes; isopropyl alcohol; lysozyme; metagenomics; microorganisms; peptidase K; polymerase chain reaction; ribosomal RNA; sewage; soil; spectroscopy; tannery waste
Abstract:
... Standardization of metagenomic DNA extraction protocol is a pre-requisite for a successful metagenomic study aiming to screen and exploit the variety of microorganisms inhabiting a particular soil environment. Six methods reported earlier were used for isolation of metagenomic DNA in the present study. These methods suffered with regard to either poor yield or quality of DNA. Therefore, we develop ...
DOI:
10.1007/s13205-017-0847-x
PubMed:
28660454
PubMed Central:
PMC5489454

6. A novel strain of Aureobasidium sp. TeO12 for theophylline production from caffeine

Author:
Ashengroph, Morahem
Source:
3 Biotech 2017 v.7 no.3 pp. 176
ISSN:
2190-572X
Subject:
Aureobasidium; biotransformation; buffers; caffeine; carbon; catalysts; high performance liquid chromatography; internal transcribed spacers; iron; nitrogen; pH; phenotype; ribosomal DNA; sequence analysis; sodium phosphate; theophylline; thin layer chromatography
Abstract:
... A total of 40 fungal cultures were isolated for their ability to grow on caffeine as a sole source carbon and nitrogen, and further screened for theophylline-producing activities under the growing cell system. Based on thin-layer chromatography and high-performance liquid chromatography analyses, the potent strain Aureobasidium sp. TeO12 was chosen for its capability to generate theophylline via b ...
DOI:
10.1007/s13205-017-0793-7
PubMed:
28664363
PubMed Central:
PMC5491430

7. A quick and effective in-house method of DNA purification from agarose gel, suitable for sequencing

Author:
Abraham, Obrador-Sánchez José; Miguel, Tzec-Sima; Inocencio, Higuera-Ciapara; Blondy, Canto-Canché
Source:
3 Biotech 2017 v.7 no.3 pp. 180
ISSN:
2190-572X
Subject:
DNA fragmentation; agarose; alleles; analytical kits; bacteria; biodiversity; developing countries; fungi; gels; genetic markers; internal transcribed spacers; molecular biology; phylogeny; plants (botany); plasmids; polymerase chain reaction; single nucleotide polymorphism
Abstract:
... Sequencing of DNA fragments (e.g., ITS, 16S, 18S, particular genes, and molecular markers) is increasingly required in studies on microbial diversity, microbial genetic population and phylogeny, sequencing of alleles, and searching for SNPs, among others. The cost of obtaining these DNAs, in quantity and quality for sequencing, is high as it involves special kits to recover DNA from gel after PCR, ...
DOI:
10.1007/s13205-017-0851-1
PubMed:
28664367
PubMed Central:
PMC5491447

8. Assessment of phytoremediation ability of Coriander sativum for soil and water co-contaminated with lead and arsenic: a small-scale study

Author:
Gaur, Nisha; Kukreja, Aayush; Yadav, Mahavir; Tiwari, Archana
Source:
3 Biotech 2017 v.7 no.3 pp. 196
ISSN:
2190-572X
Subject:
Coriandrum sativum; arsenic; lead; metal tolerance; physicochemical properties; phytoremediation; polluted soils; seed germination; seedling growth; seeds; soil pollution
Abstract:
... A study was conducted to access the phytoremediation potential of Coriandrum sativum for lead (Pb) and Arsenic (As). Metal tolerance index and pot experiment were conducted. Viable seeds were spread on filter paper and planted in soil placed in pots. The amount of Pb and As in control and in tailing soil was 0.27, 0.141, 1.77, and 0.35 ppm. The study was carried out in triplicates for a period of ...
DOI:
10.1007/s13205-017-0794-6
PubMed:
28667636
PubMed Central:
PMC5493566

9. Bacopaside N1 biosynthetic potential of endophytic Aspergillus sp. BmF 16 isolated from Bacopa monnieri

Author:
Jasim, B.; Daya, P. S.; Sreelakshmi, K. S.; Sachidanandan, P.; Aswani, R.; Jyothis, Mathew; Radhakrishnan, E. K.
Source:
3 Biotech 2017 v.7 no.3 pp. 210
ISSN:
2190-572X
Subject:
Aspergillus; Bacopa monnieri; endophytes; fungi; high performance liquid chromatography; metabolites
Abstract:
... Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. All the isolates were screened for bacopaside production property by HPLC. Among ...
DOI:
10.1007/s13205-017-0788-4
PubMed:
28667650
PubMed Central:
PMC5493564

10. Bamboo shoots as a nutritive boon for Northeast India: an overview

Author:
Basumatary, Anusriti; Middha, Sushil Kumar; Usha, Talambedu; Basumatary, Amit Kumar; Brahma, Birendra Kumar; Goyal, Arvind Kumar
Source:
3 Biotech 2017 v.7 no.3 pp. 169
ISSN:
2190-572X
Subject:
bamboo shoots; bamboos; cardiovascular diseases; diet; digestion; food security; health foods; humans; juveniles; malnutrition; minerals; neoplasms; nutritive value; proteins; shoots; sugars; weight loss; India
Abstract:
... With about 136 species, India is one among the richest countries in bamboo resources that help mankind in more than 1500 diverse ways. Although the utilization of juvenile shoots of bamboo in tribal diet of Northeast India is a very old observable fact, its numerous health benefits and potentiality remains uncharted. Apart from being delicious, the bamboo shoots are also rich in minerals and nutri ...
DOI:
10.1007/s13205-017-0796-4
PubMed:
28660452
PubMed Central:
PMC5489443

11. Bioactive L acidissima protein hydrolysates using Box–Behnken design

Author:
Sonawane, Sachin K.; Arya, Shalini S.
Source:
3 Biotech 2017 v.7 no.3 pp. 218
ISSN:
2190-572X
Subject:
2,2-diphenyl-1-picrylhydrazyl; Limonia acidissima; experimental design; hydrolysis; pH; protein content; protein hydrolysates; protein supplements; temperature
Abstract:
... This study examines the extraction and hydrolysis of proteins using single factor and Box–Behnken Design (BBD). From single factor tests, optimised extraction parameters were 1% alkali concentration, 40 °C temperature, 60 min time, and 1:20 solid to alkali ratio. Under these conditions; 924.31 mg/g of total protein was obtained from Limonia acidissima (L acidissima). The maximum degree of hydrolys ...
DOI:
10.1007/s13205-017-0862-y
PubMed:
28674843
PubMed Central:
PMC5495695

12. Bioactive profile, free-radical scavenging potential, DNA damage protection activity, and mycochemicals in Aspergillus awamori (MTCC 548) extracts: a novel report on filamentous fungi

Author:
Salar, Raj Kumar; Purewal, Sukhvinder Singh; Sandhu, Kawaljit Singh
Source:
3 Biotech 2017 v.7 no.3 pp. 164
ISSN:
2190-572X
Subject:
2,2-diphenyl-1-picrylhydrazyl; Aspergillus awamori; DNA damage; antioxidant activity; ascorbic acid; bioactive compounds; biomass; cinnamic acid; coumarins; ethanol; flavonoids; fungi; gallic acid; glucose; high performance liquid chromatography; methanol; quantitative analysis; saponins
Abstract:
... Biomass of Aspergillus awamori was investigated for mycochemicals, total phenolic compounds (TPC), condensed tannin content (CTC), free-radical scavenging potential (FRSP), and DNA damage protection activity. FRSP was determined using DPPH, ABTS, FRAP (Ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. Water (Aq), aqueous ethanol 50% (Aq ...
DOI:
10.1007/s13205-017-0834-2
PubMed:
28660460
PubMed Central:
PMC5489448

13. Biomineralisation of fipronil and its major metabolite, fipronil sulfone, by Aspergillus glaucus strain AJAG1 with enzymes studies and bioformulation

Author:
Gajendiran, Anudurga; Abraham, Jayanthi
Source:
3 Biotech 2017 v.7 no.3 pp. 212
ISSN:
2190-572X
Subject:
Aspergillus glaucus; agricultural land; alkanes; biodegradation; biomineralization; bioremediation; enzymes; esters; fipronil; fly ash; fungi; gas chromatography-mass spectrometry; insecticides; kinetics; metabolites; molasses; nucleotide sequences; peanut hulls; pollutants; ribosomal RNA; scanning electron microscopy; sequence analysis; soil
Abstract:
... Fipronil is a phenylpryazole insecticide which is extensively used for the protection of agricultural yields. However, this insecticide poses various threats to the environment. Therefore it is essential to develop an effective method to degrade or eliminate this pollutant from the environment. In this present study, a fungal strain AJAG1 capable of degrading fipronil and its metabolite, fipronil ...
DOI:
10.1007/s13205-017-0820-8
PubMed:
28667652
PubMed Central:
PMC5493570

14. Bioremediation of palm industry wastes using vermicomposting technology: its environmental application as green fertilizer

Author:
Rupani, Parveen Fatemeh; Embrandiri, Asha; Ibrahim, Mahamd Hakimi; Shahadat, Mohammad; Hansen, Sune Balle; Mansor, Nur Naha Abu
Source:
3 Biotech 2017 v.7 no.3 pp. 155
ISSN:
2190-572X
Subject:
Lumbricus rubellus; Vigna radiata; bioremediation; carbon nitrogen ratio; cost effectiveness; earthworms; industrial wastes; mung beans; nitrogen; oil mill effluents; organic wastes; phosphorus; potassium; seed germination; vermicomposting; vermicomposts
Abstract:
... Several technologies are being applied for treatment of palm oil mill wastes. Among them, the biological treatments (vermicomposting) have widely been recognized as one of the most efficient and eco-friendly methods for converting organic waste materials into valuable products. The present study focuses on vermicomposting of acidic palm oil mill effluent (POME) mixed with the palm pressed fibre (P ...
DOI:
10.1007/s13205-017-0770-1
PubMed:
28623493
PubMed Central:
PMC5473783

15. Bioremoval of arsenic (V) from aqueous solutions by chemically modified fungal biomass

Author:
Cárdenas-González, J. F.; Acosta-Rodríguez, I.; Téran-Figueroa, Y.; Rodríguez-Pérez, A. S.
Source:
3 Biotech 2017 v.7 no.3 pp. 226
ISSN:
2190-572X
Subject:
Aspergillus flavus; Aspergillus fumigatus; Cladosporium; Mucor; Paecilomyces; adsorption; aqueous solutions; arsenic; atomic absorption spectrometry; bioremediation; biosorption; fungi; iron oxides; metals; microbial biomass; mycelium; pH
Abstract:
... The biosorption of arsenic (V) on nine chemically modified biomasses (with iron oxide coated) of mycelia fungi: Aspergillus flavus III, IV and V, Aspergillus fumigatus I–II, Paecilomyces sp., Cladosporium sp., Mucor sp-1 and 2 was studied in this work. This study provides evidence that the biomasses of the fungi A. flavus, IV, III and V, Paecilomyces sp., and A. fumigatus I were very efficient at ...
DOI:
10.1007/s13205-017-0868-5
PubMed:
28681286
PubMed Central:
PMC5498367

16. Chicken egg shell as a potential substrate for production of alkaline protease by Bacillus altitudinis GVC11 and its applications

Author:
Nagamalli, Harikrishna; Sitaraman, Mahalakshmi; Kandalai, Kiran Kumar; Mudhole, Gopal Reddy
Source:
3 Biotech 2017 v.7 no.3 pp. 185
ISSN:
2190-572X
Subject:
Bacillus altitudinis; alanine; antioxidant activity; arginine; carbon; chickens; cysteine; egg shell; essential amino acids; fermentation; isoleucine; leucine; lysine; maltose; methionine; nitrogen; peptones; protein hydrolysates; proteinases; valine; yeast extract
Abstract:
... Chicken egg shell and membrane were used as substrate for production of alkaline protease by Bacillus altitudinis GVC11. Maltose as additional carbon source enhanced enzyme production up to 13%. Addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. The resultant pro ...
DOI:
10.1007/s13205-017-0801-y
PubMed:
28664373
PubMed Central:
PMC5491432

17. Cloning of BBTV (Banana Bunchy Top Virus) components and screening of BBTV using functionalized gold nanoparticles

Author:
Kumar, P.; Arun, V.; Lokeswari, T. S.
Source:
3 Biotech 2017 v.7 no.3 pp. 225
ISSN:
2190-572X
Subject:
Banana bunchy top virus; Musa; antibodies; bananas; color; enzyme-linked immunosorbent assay; enzymes; financial economics; genome; hybridization; nanogold; plant diseases and disorders; plant viruses; plasmids; quantitative polymerase chain reaction; screening
Abstract:
... Banana bunchy top virus (BBTV) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. The BBTV genome comprises of six DNA components; in this study, we have cloned the six BBTV-DNA components from one of the BBTV-infected plants (Tri-8) and were submitted to GenBank. Analysis of the BBTV DNA-R component showed that it belonged to south Pacif ...
DOI:
10.1007/s13205-017-0849-8
PubMed:
28677087
PubMed Central:
PMC5496936

18. Cloning of D-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in D-lactic acid production

Author:
Huang, Yanna; You, Chunping; Liu, Zhenmin
Source:
3 Biotech 2017 v.7 no.3 pp. 194
ISSN:
2190-572X
Subject:
Escherichia coli; Lactobacillus delbrueckii subsp. bulgaricus; anaerobic conditions; functional properties; gene expression regulation; gene overexpression; genes; lactate dehydrogenase; lactic acid; lactic acid bacteria; molecular cloning; oxygen; pyruvic acid
Abstract:
... Lactobacillus delbrueckii subsp. bulgaricus is a heterogenous lactic acid bacterium that converts pyruvate mainly to D-lactic acid using D-lactate dehydrogenases (D-LDHs), whose functional properties remain poorly characterized. Here, the D-LDHs genes (ldb0101, ldb0813, ldb1010, ldb1147 and ldb2021) were cloned and overexpressed in Escherichia coli JM109 from an inducible pUC18 vector, respectivel ...
DOI:
10.1007/s13205-017-0822-6
PubMed:
28664378
PubMed Central:
PMC5491441

19. Codon usage analysis of photolyase encoding genes of cyanobacteria inhabiting diverse habitats

Author:
Rajneesh,; Pathak, Jainendra; Kannaujiya, Vinod K.; Singh, Shailendra P.; Sinha, Rajeshwar P.
Source:
3 Biotech 2017 v.7 no.3 pp. 192
ISSN:
2190-572X
Subject:
freshwater; hot springs; principal component analysis; codons; amino acids; amino acid composition; genomics; Cyanobacteria; habitats
Abstract:
... Nucleotide and amino acid compositions were studied to determine the genomic and structural relationship of photolyase gene in freshwater, marine and hot spring cyanobacteria. Among three habitats, photolyase encoding genes from hot spring cyanobacteria were found to have highest GC content. The genomic GC content was found to influence the codon usage and amino acid variability in photolyases. Th ...
DOI:
10.1007/s13205-017-0826-2
PubMed:
28664377
PubMed Central:
PMC5491442

20. Combination of DNA isolation and RP-HPLC analysis method for bark samples of Saraca asoca and its adulterant

Author:
Hegde, Satisha; Pai, Sandeep Ramchandra; Roy, Subarna
Source:
3 Biotech 2017 v.7 no.3 pp. 208
ISSN:
2190-572X
Subject:
DNA; DNA fingerprinting; Polyalthia longifolia; Saraca asoca; bark; catechin; cetyltrimethylammonium bromide; drugs; epicatechin; filtrates; filtration; gallic acid; genetic markers; microsatellite repeats; polymerase chain reaction; quality control; reversed-phase high performance liquid chromatography; soaking; start codon
Abstract:
... DNA fingerprinting singly or in combination with phytochemical analysis is ideal for quality control of crude plant-based drugs. However, when the source material is tannin rich stem bark, extraction of DNA by conventional methods becomes challenging. In such cases, phytochemical profiling serves as very useful tool for its identification. The work herein described a method for simultaneous DNA is ...
DOI:
10.1007/s13205-017-0791-9
PubMed:
28667648
PubMed Central:
PMC5493565