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- Author:
- Tyagaturu Renukaprasad, Monisha; Ismailsab, Mukram; Reddy, Pooja V.; Baburao, Kirankumar; Nayak, Anand S.; Karegoudar, T. B.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 215
- ISSN:
- 2190-572X
- Subject:
- Pseudomonas; Stenotrophomonas; Xanthomonas; agricultural wastes; caffeic acid; carbon; energy; enrichment culture; mixed culture
- Abstract:
- ... Three bacterial strains; Pseudomonas sp. TRMK1, Stenotrophomonas sp. TRMK2 and Xanthomonas sp. TRMK3 were isolated from agro-industrial waste by enrichment culture technique that are capable of utilizing phenolic acids as sole source of carbon and energy. These strains were found to utilize p-coumaric, ferulic and caffeic acid. The individual strains utilized 5 mM of mixed phenolic acids within 20 ...
- DOI:
- 10.1007/s13205-017-0815-5
- PubMed:
- 28669074
- PubMed Central:
- PMC5494029
-
http://dx.doi.org/10.1007/s13205-017-0815-5
- Author:
- Goswami, Madhurankhi; Bhattacharyya, Purnita; Tribedi, Prosun
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 173
- ISSN:
- 2190-572X
- Subject:
- color; ecosystems; enzyme activity; fluorescein diacetate; functional diversity; gloves; hydrolysis; isoprene; landfills; microbial activity; multivariate analysis; polymers; rubber; soil; soil ecology; soil sampling
- Abstract:
- ... Rubber is a polymer of isoprene, consisting mainly of cis-1,4-polyisoprene units. The unmanageable production and its irresponsible disposal pose severe threats to environmental ecology. Therefore, the current study focuses extensively on the ill-effects of Rubber disposal on soil microbial functional diversity as it reflects the health of ecosystem by acting as a key component in ecosystem produc ...
- DOI:
- 10.1007/s13205-017-0854-y
- PubMed:
- 28660461
- PubMed Central:
- PMC5489457
-
http://dx.doi.org/10.1007/s13205-017-0854-y
- Author:
- Mun, Bong-Gyu; Lee, Sang-Uk; Park, Eung-Jun; Kim, Hyun-Ho; Hussain, Adil; Imran, Qari Muhammad; Lee, In-Jung; Yun, Byung-Wook
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 209
- ISSN:
- 2190-572X
- Subject:
- Populus; drought; forest trees; genes; nucleotide sequences; plant response; polyethylene glycol; protein structure; quantitative polymerase chain reaction; sequence analysis; stress response; transcription (genetics); transcription factors; transcriptomics; water stress; Korean Peninsula
- Abstract:
- ... Populus davidiana is native to the Korean Peninsula and is one of the most dominant and abundantly growing forest trees in eastern Asia. Compared to other Populus species such as P. trichocarpa, P. euphratica, and P. tremula, relatively little is known about P. davidiana. Here, we performed transcriptomic analysis of P. davidiana under drought stress induced by 10% polyethylene glycol. A total of ...
- DOI:
- 10.1007/s13205-017-0858-7
- PubMed:
- 28667649
- PubMed Central:
- PMC5493580
-
http://dx.doi.org/10.1007/s13205-017-0858-7
- Author:
- Kumar, Dhirendra; Dubey, Kashyap Kumar
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 157
- ISSN:
- 2190-572X
- Subject:
- Bacillus megaterium; antineoplastic activity; antiretroviral agents; betulin; betulinic acid; biomass; biotransformation; culture flasks; fermenters; moieties; pH; selective media; statistical analysis; temperature
- Abstract:
- ... Betulinic acid as a derivative of betulin is widely reported for its anti-HIV and antitumor activities. Betulin has three most significant positions, i.e., primary hydroxyl group at position C-28, secondary hydroxyl group at position C-3, and alkene moiety at position C-20, where chemical modifications were performed to yield pharmacologically more active derivatives. Bioconversion optimization wa ...
- DOI:
- 10.1007/s13205-017-0759-9
- PubMed:
- 28623491
- PubMed Central:
- PMC5473782
-
http://dx.doi.org/10.1007/s13205-017-0759-9
- Author:
- Verma, Sumit Kumar; Singh, Himanshi; Sharma, Prakash C.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 171
- ISSN:
- 2190-572X
- Subject:
- DNA; calcium chloride; cellulosic wastes; cetyltrimethylammonium bromide; edaphic factors; gardens; genes; isopropyl alcohol; lysozyme; metagenomics; microorganisms; peptidase K; polymerase chain reaction; ribosomal RNA; sewage; soil; spectroscopy; tannery waste
- Abstract:
- ... Standardization of metagenomic DNA extraction protocol is a pre-requisite for a successful metagenomic study aiming to screen and exploit the variety of microorganisms inhabiting a particular soil environment. Six methods reported earlier were used for isolation of metagenomic DNA in the present study. These methods suffered with regard to either poor yield or quality of DNA. Therefore, we develop ...
- DOI:
- 10.1007/s13205-017-0847-x
- PubMed:
- 28660454
- PubMed Central:
- PMC5489454
-
http://dx.doi.org/10.1007/s13205-017-0847-x
- Author:
- Ashengroph, Morahem
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 176
- ISSN:
- 2190-572X
- Subject:
- Aureobasidium; biotransformation; buffers; caffeine; carbon; catalysts; high performance liquid chromatography; internal transcribed spacers; iron; nitrogen; pH; phenotype; ribosomal DNA; sequence analysis; sodium phosphate; theophylline; thin layer chromatography
- Abstract:
- ... A total of 40 fungal cultures were isolated for their ability to grow on caffeine as a sole source carbon and nitrogen, and further screened for theophylline-producing activities under the growing cell system. Based on thin-layer chromatography and high-performance liquid chromatography analyses, the potent strain Aureobasidium sp. TeO12 was chosen for its capability to generate theophylline via b ...
- DOI:
- 10.1007/s13205-017-0793-7
- PubMed:
- 28664363
- PubMed Central:
- PMC5491430
-
http://dx.doi.org/10.1007/s13205-017-0793-7
- Author:
- Abraham, Obrador-Sánchez José; Miguel, Tzec-Sima; Inocencio, Higuera-Ciapara; Blondy, Canto-Canché
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 180
- ISSN:
- 2190-572X
- Subject:
- DNA fragmentation; agarose; alleles; analytical kits; bacteria; biodiversity; developing countries; fungi; gels; genetic markers; internal transcribed spacers; molecular biology; phylogeny; plants (botany); plasmids; polymerase chain reaction; single nucleotide polymorphism
- Abstract:
- ... Sequencing of DNA fragments (e.g., ITS, 16S, 18S, particular genes, and molecular markers) is increasingly required in studies on microbial diversity, microbial genetic population and phylogeny, sequencing of alleles, and searching for SNPs, among others. The cost of obtaining these DNAs, in quantity and quality for sequencing, is high as it involves special kits to recover DNA from gel after PCR, ...
- DOI:
- 10.1007/s13205-017-0851-1
- PubMed:
- 28664367
- PubMed Central:
- PMC5491447
-
http://dx.doi.org/10.1007/s13205-017-0851-1
- Author:
- Gaur, Nisha; Kukreja, Aayush; Yadav, Mahavir; Tiwari, Archana
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 196
- ISSN:
- 2190-572X
- Subject:
- Coriandrum sativum; arsenic; lead; metal tolerance; physicochemical properties; phytoremediation; polluted soils; seed germination; seedling growth; seeds; soil pollution
- Abstract:
- ... A study was conducted to access the phytoremediation potential of Coriandrum sativum for lead (Pb) and Arsenic (As). Metal tolerance index and pot experiment were conducted. Viable seeds were spread on filter paper and planted in soil placed in pots. The amount of Pb and As in control and in tailing soil was 0.27, 0.141, 1.77, and 0.35 ppm. The study was carried out in triplicates for a period of ...
- DOI:
- 10.1007/s13205-017-0794-6
- PubMed:
- 28667636
- PubMed Central:
- PMC5493566
-
http://dx.doi.org/10.1007/s13205-017-0794-6
- Author:
- Jasim, B.; Daya, P. S.; Sreelakshmi, K. S.; Sachidanandan, P.; Aswani, R.; Jyothis, Mathew; Radhakrishnan, E. K.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 210
- ISSN:
- 2190-572X
- Subject:
- Aspergillus; Bacopa monnieri; endophytes; fungi; high performance liquid chromatography; metabolites
- Abstract:
- ... Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. All the isolates were screened for bacopaside production property by HPLC. Among ...
- DOI:
- 10.1007/s13205-017-0788-4
- PubMed:
- 28667650
- PubMed Central:
- PMC5493564
-
http://dx.doi.org/10.1007/s13205-017-0788-4
- Author:
- Basumatary, Anusriti; Middha, Sushil Kumar; Usha, Talambedu; Basumatary, Amit Kumar; Brahma, Birendra Kumar; Goyal, Arvind Kumar
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 169
- ISSN:
- 2190-572X
- Subject:
- bamboo shoots; bamboos; cardiovascular diseases; diet; digestion; food security; health foods; humans; juveniles; malnutrition; minerals; neoplasms; nutritive value; proteins; shoots; sugars; weight loss; India
- Abstract:
- ... With about 136 species, India is one among the richest countries in bamboo resources that help mankind in more than 1500 diverse ways. Although the utilization of juvenile shoots of bamboo in tribal diet of Northeast India is a very old observable fact, its numerous health benefits and potentiality remains uncharted. Apart from being delicious, the bamboo shoots are also rich in minerals and nutri ...
- DOI:
- 10.1007/s13205-017-0796-4
- PubMed:
- 28660452
- PubMed Central:
- PMC5489443
-
http://dx.doi.org/10.1007/s13205-017-0796-4
- Author:
- Sonawane, Sachin K.; Arya, Shalini S.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 218
- ISSN:
- 2190-572X
- Subject:
- 2,2-diphenyl-1-picrylhydrazyl; Limonia acidissima; experimental design; hydrolysis; pH; protein content; protein hydrolysates; protein supplements; temperature
- Abstract:
- ... This study examines the extraction and hydrolysis of proteins using single factor and Box–Behnken Design (BBD). From single factor tests, optimised extraction parameters were 1% alkali concentration, 40 °C temperature, 60 min time, and 1:20 solid to alkali ratio. Under these conditions; 924.31 mg/g of total protein was obtained from Limonia acidissima (L acidissima). The maximum degree of hydrolys ...
- DOI:
- 10.1007/s13205-017-0862-y
- PubMed:
- 28674843
- PubMed Central:
- PMC5495695
-
http://dx.doi.org/10.1007/s13205-017-0862-y
- Author:
- Salar, Raj Kumar; Purewal, Sukhvinder Singh; Sandhu, Kawaljit Singh
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 164
- ISSN:
- 2190-572X
- Subject:
- 2,2-diphenyl-1-picrylhydrazyl; Aspergillus awamori; DNA damage; antioxidant activity; ascorbic acid; bioactive compounds; biomass; cinnamic acid; coumarins; ethanol; flavonoids; fungi; gallic acid; glucose; high performance liquid chromatography; methanol; quantitative analysis; saponins
- Abstract:
- ... Biomass of Aspergillus awamori was investigated for mycochemicals, total phenolic compounds (TPC), condensed tannin content (CTC), free-radical scavenging potential (FRSP), and DNA damage protection activity. FRSP was determined using DPPH, ABTS, FRAP (Ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. Water (Aq), aqueous ethanol 50% (Aq ...
- DOI:
- 10.1007/s13205-017-0834-2
- PubMed:
- 28660460
- PubMed Central:
- PMC5489448
-
http://dx.doi.org/10.1007/s13205-017-0834-2
- Author:
- Gajendiran, Anudurga; Abraham, Jayanthi
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 212
- ISSN:
- 2190-572X
- Subject:
- Aspergillus glaucus; agricultural land; alkanes; biodegradation; biomineralization; bioremediation; enzymes; esters; fipronil; fly ash; fungi; gas chromatography-mass spectrometry; insecticides; kinetics; metabolites; molasses; nucleotide sequences; peanut hulls; pollutants; ribosomal RNA; scanning electron microscopy; sequence analysis; soil
- Abstract:
- ... Fipronil is a phenylpryazole insecticide which is extensively used for the protection of agricultural yields. However, this insecticide poses various threats to the environment. Therefore it is essential to develop an effective method to degrade or eliminate this pollutant from the environment. In this present study, a fungal strain AJAG1 capable of degrading fipronil and its metabolite, fipronil ...
- DOI:
- 10.1007/s13205-017-0820-8
- PubMed:
- 28667652
- PubMed Central:
- PMC5493570
-
http://dx.doi.org/10.1007/s13205-017-0820-8
- Author:
- Rupani, Parveen Fatemeh; Embrandiri, Asha; Ibrahim, Mahamd Hakimi; Shahadat, Mohammad; Hansen, Sune Balle; Mansor, Nur Naha Abu
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 155
- ISSN:
- 2190-572X
- Subject:
- Lumbricus rubellus; Vigna radiata; bioremediation; carbon nitrogen ratio; cost effectiveness; earthworms; industrial wastes; mung beans; nitrogen; oil mill effluents; organic wastes; phosphorus; potassium; seed germination; vermicomposting; vermicomposts
- Abstract:
- ... Several technologies are being applied for treatment of palm oil mill wastes. Among them, the biological treatments (vermicomposting) have widely been recognized as one of the most efficient and eco-friendly methods for converting organic waste materials into valuable products. The present study focuses on vermicomposting of acidic palm oil mill effluent (POME) mixed with the palm pressed fibre (P ...
- DOI:
- 10.1007/s13205-017-0770-1
- PubMed:
- 28623493
- PubMed Central:
- PMC5473783
-
http://dx.doi.org/10.1007/s13205-017-0770-1
- Author:
- Cárdenas-González, J. F.; Acosta-Rodríguez, I.; Téran-Figueroa, Y.; Rodríguez-Pérez, A. S.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 226
- ISSN:
- 2190-572X
- Subject:
- Aspergillus flavus; Aspergillus fumigatus; Cladosporium; Mucor; Paecilomyces; adsorption; aqueous solutions; arsenic; atomic absorption spectrometry; bioremediation; biosorption; fungi; iron oxides; metals; microbial biomass; mycelium; pH
- Abstract:
- ... The biosorption of arsenic (V) on nine chemically modified biomasses (with iron oxide coated) of mycelia fungi: Aspergillus flavus III, IV and V, Aspergillus fumigatus I–II, Paecilomyces sp., Cladosporium sp., Mucor sp-1 and 2 was studied in this work. This study provides evidence that the biomasses of the fungi A. flavus, IV, III and V, Paecilomyces sp., and A. fumigatus I were very efficient at ...
- DOI:
- 10.1007/s13205-017-0868-5
- PubMed:
- 28681286
- PubMed Central:
- PMC5498367
-
http://dx.doi.org/10.1007/s13205-017-0868-5
- Author:
- Nagamalli, Harikrishna; Sitaraman, Mahalakshmi; Kandalai, Kiran Kumar; Mudhole, Gopal Reddy
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 185
- ISSN:
- 2190-572X
- Subject:
- Bacillus altitudinis; alanine; antioxidant activity; arginine; carbon; chickens; cysteine; egg shell; essential amino acids; fermentation; isoleucine; leucine; lysine; maltose; methionine; nitrogen; peptones; protein hydrolysates; proteinases; valine; yeast extract
- Abstract:
- ... Chicken egg shell and membrane were used as substrate for production of alkaline protease by Bacillus altitudinis GVC11. Maltose as additional carbon source enhanced enzyme production up to 13%. Addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. The resultant pro ...
- DOI:
- 10.1007/s13205-017-0801-y
- PubMed:
- 28664373
- PubMed Central:
- PMC5491432
-
http://dx.doi.org/10.1007/s13205-017-0801-y
- Author:
- Kumar, P.; Arun, V.; Lokeswari, T. S.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 225
- ISSN:
- 2190-572X
- Subject:
- Banana bunchy top virus; Musa; antibodies; bananas; color; enzyme-linked immunosorbent assay; enzymes; financial economics; genome; hybridization; nanogold; plant diseases and disorders; plant viruses; plasmids; quantitative polymerase chain reaction; screening
- Abstract:
- ... Banana bunchy top virus (BBTV) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. The BBTV genome comprises of six DNA components; in this study, we have cloned the six BBTV-DNA components from one of the BBTV-infected plants (Tri-8) and were submitted to GenBank. Analysis of the BBTV DNA-R component showed that it belonged to south Pacif ...
- DOI:
- 10.1007/s13205-017-0849-8
- PubMed:
- 28677087
- PubMed Central:
- PMC5496936
-
http://dx.doi.org/10.1007/s13205-017-0849-8
- Author:
- Huang, Yanna; You, Chunping; Liu, Zhenmin
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 194
- ISSN:
- 2190-572X
- Subject:
- Escherichia coli; Lactobacillus delbrueckii subsp. bulgaricus; anaerobic conditions; functional properties; gene expression regulation; gene overexpression; genes; lactate dehydrogenase; lactic acid; lactic acid bacteria; molecular cloning; oxygen; pyruvic acid
- Abstract:
- ... Lactobacillus delbrueckii subsp. bulgaricus is a heterogenous lactic acid bacterium that converts pyruvate mainly to D-lactic acid using D-lactate dehydrogenases (D-LDHs), whose functional properties remain poorly characterized. Here, the D-LDHs genes (ldb0101, ldb0813, ldb1010, ldb1147 and ldb2021) were cloned and overexpressed in Escherichia coli JM109 from an inducible pUC18 vector, respectivel ...
- DOI:
- 10.1007/s13205-017-0822-6
- PubMed:
- 28664378
- PubMed Central:
- PMC5491441
-
http://dx.doi.org/10.1007/s13205-017-0822-6
- Author:
- Rajneesh,; Pathak, Jainendra; Kannaujiya, Vinod K.; Singh, Shailendra P.; Sinha, Rajeshwar P.
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 192
- ISSN:
- 2190-572X
- Subject:
- freshwater; hot springs; principal component analysis; codons; amino acids; amino acid composition; genomics; Cyanobacteria; habitats
- Abstract:
- ... Nucleotide and amino acid compositions were studied to determine the genomic and structural relationship of photolyase gene in freshwater, marine and hot spring cyanobacteria. Among three habitats, photolyase encoding genes from hot spring cyanobacteria were found to have highest GC content. The genomic GC content was found to influence the codon usage and amino acid variability in photolyases. Th ...
- DOI:
- 10.1007/s13205-017-0826-2
- PubMed:
- 28664377
- PubMed Central:
- PMC5491442
-
http://dx.doi.org/10.1007/s13205-017-0826-2
- Author:
- Hegde, Satisha; Pai, Sandeep Ramchandra; Roy, Subarna
- Source:
- 3 Biotech 2017 v.7 no.3 pp. 208
- ISSN:
- 2190-572X
- Subject:
- DNA; DNA fingerprinting; Polyalthia longifolia; Saraca asoca; bark; catechin; cetyltrimethylammonium bromide; drugs; epicatechin; filtrates; filtration; gallic acid; genetic markers; microsatellite repeats; polymerase chain reaction; quality control; reversed-phase high performance liquid chromatography; soaking; start codon
- Abstract:
- ... DNA fingerprinting singly or in combination with phytochemical analysis is ideal for quality control of crude plant-based drugs. However, when the source material is tannin rich stem bark, extraction of DNA by conventional methods becomes challenging. In such cases, phytochemical profiling serves as very useful tool for its identification. The work herein described a method for simultaneous DNA is ...
- DOI:
- 10.1007/s13205-017-0791-9
- PubMed:
- 28667648
- PubMed Central:
- PMC5493565
-
http://dx.doi.org/10.1007/s13205-017-0791-9