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Acta horticulturae
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7982-2018
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2018 no.1224
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- Author:
- J. C. A. Hiti Bandaralage; A. Hayward; C. O'Brien; C. Beveridge; N. Mitter
- Source:
- Acta horticulturae 2018 no.1224 pp. 13-20
- ISSN:
- 0567-7572
- Subject:
- Persea americana; acclimation; avocados; humidity; lids; micropropagation; mists; nutritive value; plantlets; recalcitrant species; rooting; soaking; survival rate; tissue culture; woody plants
- Abstract:
- ... The morphology, anatomy and physiology of in vitro cultured plants do not facilitate survival when transferred to dynamic ex vitro environments. The benefits of a micropropagation system completely depend on successful transfer of in vitro plants to septic ex vitro conditions. Avocado (Persea americana Mill.) is a luxurious tropical fruit becoming very popular with increasing consumption around th ...
- DOI:
- 10.17660/ActaHortic.2018.1224.3
- https://doi.org/10.17660/ActaHortic.2018.1224.3
- Author:
- C. S. Schuchovski; L. A. Biasi
- Source:
- Acta horticulturae 2018 no.1224 pp. 157-164
- ISSN:
- 0567-7572
- Subject:
- abnormal development; benzyladenine; blackberries; callus formation; culture media; cytokinins; gene transfer; germplasm conservation; in vitro culture; kinetin; leaves; plantlets; protocols; salts; shoots; thidiazuron; woody plants; zeatin
- Abstract:
- ... In vitro culture is a very important technique in plant mass propagation, as well as an auxiliary tool to germplasm conservation and gene transfer. The present study developed an efficient multiplication protocol for 'Brazos' blackberry. Here, its multiplication ability was evaluated using in vitro shoots grown on medium supplemented with different growth regulators: thidiazuron (TDZ), 6-benzylami ...
- DOI:
- 10.17660/ActaHortic.2018.1224.21
- https://doi.org/10.17660/ActaHortic.2018.1224.21
- Author:
- P. A. A. Santos; G. S. Dias; A. M. C. Mendonca; C. D. Silva Junior; L. C. Silva; M. C. Santana
- Source:
- Acta horticulturae 2018 no.1224 pp. 39-44
- ISSN:
- 0567-7572
- Subject:
- Spondias tuberosa; caatinga; chlorine; culture media; disinfection; dormancy; excision; experimental design; explants; fruit trees; fruits; germination; gibberellic acid; leaves; ovule culture; plantations; roots; seedlings; seeds; shoots; sodium hypochlorite; stem elongation
- Abstract:
- ... The umbuzeiro (Spondias tuberosa Arruda) is a native fruit tree of Caatinga and has great economic importance. Its fruits are collected extractivistically due to lack of commercial plantations. Furthermore, the seeds have physical dormancy, which reduces germination rates. However, tissue culture can be used to overcome such problems by excision and in vitro culture of embryos with gibberellin. Th ...
- DOI:
- 10.17660/ActaHortic.2018.1224.6
- https://doi.org/10.17660/ActaHortic.2018.1224.6
- Author:
- R. F. Jr. Galdiano; E. G. M. Lemos
- Source:
- Acta horticulturae 2018 no.1224 pp. 135-138
- ISSN:
- 0567-7572
- Subject:
- Cattleya labiata; acclimation; air flow; cryopreservation; dimethyl sulfoxide; ethylene glycol; genetic resources; glycerol; greenhouses; habitat destruction; liquids; pH; seedlings; seeds; sodium; sucrose
- Abstract:
- ... Orchid populations have been decreasing due to habitat loss and over-collection for ornamental use. Consequently, there is an urgent need to conserve germplasm resources. Cryopreservation of the threatened orchid Cattleya labiata L. was successfully achieved using encapsulation-vitrification and encapsulation-dehydration cryopreservation methods. Protocorms, of 0.1 cm in diameter, developed from 2 ...
- DOI:
- 10.17660/ActaHortic.2018.1224.18
- https://doi.org/10.17660/ActaHortic.2018.1224.18
- Author:
- A. D. Arencibia; A. Gomez; M. A. Poblete; F. Orellana; J. E. Alarcon; N. Cortez; M. A. Valenzuela
- Source:
- Acta horticulturae 2018 no.1224 pp. 203-208
- ISSN:
- 0567-7572
- Subject:
- Populus; Rubus idaeus; Vaccinium corymbosum; acclimation; bioreactors; blueberries; carbon dioxide; light intensity; micropropagation; raspberries
- Abstract:
- ... The optimization of photomixotrophic conditions (increase of both light intensity and the CO(2) atmosphere inside the culture flask) improves plant quality and can lead to greater efficiency during the acclimatization phase. For this case, temporary immersion bioreactors (TIBs) installed in controlled environment characterized by CO(2) enrichment, higher luminosity, and sucrose-reduced medium migh ...
- DOI:
- 10.17660/ActaHortic.2018.1224.27
- https://doi.org/10.17660/ActaHortic.2018.1224.27
- Author:
- E. Yahyaoui; D. Casamento; A. M. D'Onghia; T. Elbeaino; D. Frasheri; M. A. Germana
- Source:
- Acta horticulturae 2018 no.1224 pp. 81-90
- ISSN:
- 0567-7572
- Subject:
- DNA viruses; Ficus carica; Fig badnavirus 1; Fig mosaic virus; cultivars; encapsulation; genotype; leaves; meristems; plantlets; reverse transcriptase polymerase chain reaction; sanitation; survival rate; virus-free plants; viruses
- Abstract:
- ... Three Mediterranean Ficus carica genotypes, i.e., cultivars ‘Palazzo’, ‘Bifera nera’ and ‘Catalanisca’, initially infected by Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2), Fig mild mottling-associated virus (FMMaV), Fig mosaic virus (FMV), Fig latent virus 1 (FLV-1), Fig badnavirus 1 (FBV-1) and Fig fleck-associated virus (FFkaV), were subjected to the ...
- DOI:
- 10.17660/ActaHortic.2018.1224.12
- https://doi.org/10.17660/ActaHortic.2018.1224.12
- Author:
- J. P. Alvarenga; F. V. Pacheco; S. K. V. Bertolucci; S. T. Silva; T. de Oliveira; J. E. B. P. Pinto
- Source:
- Acta horticulturae 2018 no.1224 pp. 175-182
- ISSN:
- 0567-7572
- Subject:
- Mentha spicata subsp. spicata; dry matter accumulation; essential oils; explants; fluorescent lighting; growth regulators; headspace analysis; leaves; light emitting diodes; light intensity; pharmaceutical industry; photons; photosynthesis; plantlets; volatile compounds
- Abstract:
- ... Mentha viridis (Lamiaceae) has a great potential for economic exploration due to its essential oil production, which is widely used in chemical and pharmaceutical industries. The aim of this work was to study the effect of light spectra, in the presence and absence of growth regulators (BAP), and light intensity on M. viridis growth and production of volatile compounds under in vitro conditions. N ...
- DOI:
- 10.17660/ActaHortic.2018.1224.23
- https://doi.org/10.17660/ActaHortic.2018.1224.23
- Author:
- S. Ghorbani; E. Mignon; S. P. O. Werbrouck
- Source:
- Acta horticulturae 2018 no.1224 pp. 191-194
- ISSN:
- 0567-7572
- Subject:
- Hevea brasiliensis; callus; explants; field experimentation; fruits; funding; gardens; integument; juvenility; meristems; micropropagation; mutation; plantations; root systems; rubber; seedlings; seeds; shoots; somaclonal variation; somatic embryogenesis; trees; Cote d'Ivoire; Indonesia
- Abstract:
- ... Microcutting is a repeated process in which in vitro shoots develop new shoots from axillary meristems. In Hevea, this system works for seedlings, but not for shoots derived from mature trees. As for a lot of other tree species, one of the consequences of aging (losing juvenility) is poor in vitro growth and multiplication and the lack of a proper geotropic (tap)root system. In rubber tree, somati ...
- DOI:
- 10.17660/ActaHortic.2018.1224.25
- https://doi.org/10.17660/ActaHortic.2018.1224.25
- Author:
- F. C. Nery; D. O. Prudente; R. Paiva; M. C. Nery; P. D. O. Paiva; D. Domiciano
- Source:
- Acta horticulturae 2018 no.1224 pp. 183-190
- ISSN:
- 0567-7572
- Subject:
- Pectobacterium carotovorum; Zantedeschia aethiopica; absorbance; acclimation; agar; bacterial infections; benzyladenine; callus; culture media; dyes; explants; floriculture; flowers; histology; indole butyric acid; leaves; micropropagation; ornamental plants; plantlets; production costs; rooting; shoots; sucrose; viability assays; Brazil
- Abstract:
- ... The expansion of floriculture in Brazil indicates that producers need to specialize and find strategies to reduce production costs and improve the quality of commercial ornamental plants. Calla lily is considered as one of the most appreciated flowers in Brazil. However, there are problems mainly related to bacterial infection caused by Pectobacterium carotovorum. Thus, this study aimed to assess ...
- DOI:
- 10.17660/ActaHortic.2018.1224.24
- https://doi.org/10.17660/ActaHortic.2018.1224.24
- Author:
- V. Reinhart; L. A. Biasi
- Source:
- Acta horticulturae 2018 no.1224 pp. 127-134
- ISSN:
- 0567-7572
- Subject:
- Vitis labrusca; Vitis rotundifolia; acclimation; bottles; cutting; explants; hybrids; indole butyric acid; leaves; micropropagation; mists; naphthaleneacetic acid; rhizogenesis; rooting; shoots; survival rate; vermiculite
- Abstract:
- ... Rhizogenesis is an important step in micropropagation and root quality is essential for plant survival during acclimatization. Vitis rotundifolia hybrids show low rooting rate and auxin application is important for cutting propagation. This study was aimed at evaluating different concentrations of naphthalene acetic acid (NAA), 0.0, 0.2, 0.4 and 0.8 µM, in in vitro rooting and different concentrat ...
- DOI:
- 10.17660/ActaHortic.2018.1224.17
- https://doi.org/10.17660/ActaHortic.2018.1224.17
- Author:
- R. M. Moraes; L. B. Souza; F. C. Nery; R. Paiva; S. Barbosa
- Source:
- Acta horticulturae 2018 no.1224 pp. 165-174
- ISSN:
- 0567-7572
- Subject:
- Hibiscus sabdariffa; acclimation; cooking; cryopreservation; cryoprotectants; experimental design; explants; germination; germplasm; histology; protocols; rooting; seed moisture; seedlings; seeds; shoots; silica gel; water content
- Abstract:
- ... Hibiscus sabdariffa L. (Malvaceae) is a species widely exploited for cooking, ornamental and medicinal purposes, which justifies the efforts for its maintenance and conservation. The objective of this study was to establish a cryopreservation protocol for the conservation of H. sabdariffa seeds. The success of cryopreservation was investigated by the evaluation of seed moisture content (MC%) and, ...
- DOI:
- 10.17660/ActaHortic.2018.1224.22
- https://doi.org/10.17660/ActaHortic.2018.1224.22
- Author:
- P. Jourdan
- Source:
- Acta horticulturae 2018 no.1224 pp. 31-38
- ISSN:
- 0567-7572
- Subject:
- Begonia; Phlox; USDA National Plant Germplasm System; cryopreservation; germplasm conservation; ornamental plants; plant germplasm; seed set; seeds; tissue culture
- Abstract:
- ... Seed banking remains the primary strategy for germplasm conservation. However, for selected species, in vitro shoot cultures constitute an important tool in the preservation of germplasm. Although cryopreservation is the preferred method of long-term conservation, short- and medium-term preservation with in vitro shoot cultures remains a workable and flexible option. In addition, rapid in vitro mu ...
- DOI:
- 10.17660/ActaHortic.2018.1224.5
- https://doi.org/10.17660/ActaHortic.2018.1224.5
- Author:
- E. Klocke; T. Nothnagel; R. Kramer; H. Budahn
- Source:
- Acta horticulturae 2018 no.1224 pp. 221-228
- ISSN:
- 0567-7572
- Subject:
- 2,4-D; Asparagus officinalis; Asparagus virus 1; DNA; DNA fingerprinting; acetic acid; cultivars; culture media; diet; enzyme-linked immunosorbent assay; flow cytometry; fluorescence; glutamine; greenhouses; liquids; naphthaleneacetic acid; polyethylene glycol; protocols; protoplasts; random amplified polymorphic DNA technique; somatic hybridization; vegetables
- Abstract:
- ... Asparagus is a valued vegetable in people's diet in many countries of the world. The production of asparagus increased remarkably in the last few years. Comprehensive evaluations have shown that there exist no cultivars of A. officinalis with resistance against Asparagus virus 1 (AV-1) causing a high loss of yield and quality. Resistance was found in wild species of the genus Asparagus such as in ...
- DOI:
- 10.17660/ActaHortic.2018.1224.30
- https://doi.org/10.17660/ActaHortic.2018.1224.30
14. Use of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum
- Author:
- A. Castillo; B. Lopez Carro; M. Dalla Rizza; R. Reyno
- Source:
- Acta horticulturae 2018 no.1224 pp. 73-80
- ISSN:
- 0567-7572
- Subject:
- C3 plants; C4 plants; Trifolium; antimitotic agents; autotetraploidy; biomass production; chloroplasts; chromosome duplication; colchicine; culture media; ecotypes; explants; flow cytometry; forage legumes; grasses; grazing; guard cells; in vitro studies; indigenous species; oryzalin; perennials; seedlings; vegetation; vigor; winter
- Abstract:
- ... The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum belongs to the Campos region and has evolved into adapted ecotypes through long ...
- DOI:
- 10.17660/ActaHortic.2018.1224.11
- https://doi.org/10.17660/ActaHortic.2018.1224.11
- Author:
- P. C. Khai; T. V. Minh
- Source:
- Acta horticulturae 2018 no.1224 pp. 109-118
- ISSN:
- 0567-7572
- Subject:
- Gynostemma pentaphyllum; acetic acid; adventitious roots; biomass; callus; diabetes; explants; indole butyric acid; leaves; liquids; medicinal plants; methyl jasmonate; neoplasms; plantlets; rooting; saponins; thrombosis
- Abstract:
- ... Giao Co Lam (Gynostemma pentaphyllum) is a rare valuable medicinal herb used for the human being's health as it has such medical functions as anti-stress properties, restoring the body's balance, improving memory, preventing thrombosis, inhibiting growth of tumors, and treating diabetes, etc. However, that productivity is still limited and could not meet consumer requirements. Nowadays, the develo ...
- DOI:
- 10.17660/ActaHortic.2018.1224.15
- https://doi.org/10.17660/ActaHortic.2018.1224.15
- Author:
- L. R. G. Guilherme; A. S. Lopes; A. P. Corguinha
- Source:
- Acta horticulturae 2018 no.1224 pp. 1-6
- ISSN:
- 0567-7572
- Subject:
- agricultural land; agroecosystems; climate change; ecosystem services; employment; food security; fuels; income; land productivity; outreach; soil; soil fertility; soil organic matter; subtropics; sustainable agriculture; technology transfer; tropical agriculture; tropics; Brazil
- Abstract:
- ... Brazil has been recognized as a global player in the production of food, feed, fuel, and fibers (4Fs), thanks to the use of scientifically sound technologies that allowed our agro-ecosytems to increase yields in a sustainable way. Indeed, agricultural activities are very relevant in Brazil not only because they provide jobs, income, and welfare for many Brazilians, but also due to the fact that mu ...
- DOI:
- 10.17660/ActaHortic.2018.1224.1
- https://doi.org/10.17660/ActaHortic.2018.1224.1
- Author:
- P. Morales; M. J. Montanola
- Source:
- Acta horticulturae 2018 no.1224 pp. 229-234
- ISSN:
- 0567-7572
- Subject:
- Nolana; autumn; bacteria; coasts; dry matter accumulation; flowering; fungi; in vitro culture; indole butyric acid; leaves; micropropagation; protocols; rooting; shoots; soil; water content; Chile
- Abstract:
- ... Nolana carnosa (Lindl.) Miers ex Dunal, (Fam.: Solanaceae), is a bushy plant native from Chile's north coast. It grows in the Atacama Desert in salty soils under extremely dry conditions. This understudied species is an interesting new ornamental option because of its morphological characteristics like abundant white to deep blue flowering, evergreen foliage and compact growth. With the aim to dev ...
- DOI:
- 10.17660/ActaHortic.2018.1224.31
- https://doi.org/10.17660/ActaHortic.2018.1224.31
- Author:
- J. H. Wu
- Source:
- Acta horticulturae 2018 no.1224 pp. 195-202
- ISSN:
- 0567-7572
- Subject:
- Actinidia chinensis; Actinidia deliciosa; bacterial canker; biotechnology; breeding; breeding programs; canes; chromosomes; color; commercialization; cultivars; food research; fruits; genomics; genotype; germplasm; in vitro culture; kiwifruit; markets; mature plants; micropropagation; petioles; planting; New Zealand
- Abstract:
- ... During the past 20 years kiwifruit has expanded dramatically in planting area and location, in total production, and also in diversity with different types of fruit. For example, variations in flesh colour have resulted in the commercialisation of green-fleshed, yellow-fleshed and red-fleshed kiwifruit. This diversity is from two large-fruited Actinidia species, A. chinensis and A. deliciosa, now ...
- DOI:
- 10.17660/ActaHortic.2018.1224.26
- https://doi.org/10.17660/ActaHortic.2018.1224.26
- Author:
- C. Naranjo; E. Fallas
- Source:
- Acta horticulturae 2018 no.1224 pp. 217-220
- ISSN:
- 0567-7572
- Subject:
- Manihot esculenta; Sphagnum; acclimation; blue light; buds; cassava; farmers; fluorescent lighting; greenhouses; indole butyric acid; internodes; light intensity; mortality; mosses and liverworts; peat; perlite; plantations; plantlets; protocols; red light; root cuttings; roots; sand; seeds; Costa Rica
- Abstract:
- ... A common practice during cassava cultivation in Costa Rica is the use of stem cuttings coming from farmers' plantations as vegetative seeds. This system has widely spread diseases all over the country, especially the phytoplasm associated “Frog Skin” disease. At Tecnológico de Costa Rica we developed a protocol for rapid multiplication of disease-free plantlets. Terminal buds obtained from in vitr ...
- DOI:
- 10.17660/ActaHortic.2018.1224.29
- https://doi.org/10.17660/ActaHortic.2018.1224.29
- Author:
- V. C. Stein; M. D. Cardoso Junior; M. C. Coimbra; I. Rodrigues-Brandao; M. S. Padua; A. H. F. Castro
- Source:
- Acta horticulturae 2018 no.1224 pp. 119-126
- ISSN:
- 0567-7572
- Subject:
- Bauhinia; agar; benzyladenine; culture media; environmental factors; explants; gibberellic acid; histology; leaves; legumes; micropropagation; oxidation; protocols; savannas; seed germination; seedlings; seeds; shoots; vascular bundles
- Abstract:
- ... Bauhinia holophylla (Bong.) Steud. is an endemic legume of the Brazilian Savanna that has economic and medicinal importance. The natural and conventional propagation occurs through seeds, whose production occurs during a few months per year. Currently, the Brazilian Savanna agricultural expansion aggravates the propagation and conservation of B. holophylla species. Considering the few studies abou ...
- DOI:
- 10.17660/ActaHortic.2018.1224.16
- https://doi.org/10.17660/ActaHortic.2018.1224.16