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1. A euryhaline Nannochloropsis gaditana with potential for nutraceutical (EPA) and biodiesel production

Author:
Mitra, Madhusree; Patidar, Shailesh Kumar; George, Basil; Shah, Freny; Mishra, Sandhya
Source:
Algal research 2015 v.8 pp. 161-167
ISSN:
2211-9264
Subject:
Nannochloropsis; biodiesel; biomass; carotenoids; chlorophyll; eicosapentaenoic acid; euryhaline species; feedstocks; fuel production; functional foods; light intensity; microalgae; monounsaturated fatty acids; nutrient content; oils; photoperiod; principal component analysis; salinity
Abstract:
... The holy grail of the present work was to study the effects of salinity (20, 30, 35, and 40g/L), light intensity (60 and 150μmolphotonsm−2s−1) and photoperiod (24h:0h, 18h:06h, 12h:12h, 06h:18h and 00h:24h light:dark) on selective nutraceutical properties of Nannochloropsis gaditana with a focus on eicosapentaenoic acid production. The maximal biomass (45.01±1.01mgL−1day−1) and lipid productivitie ...
DOI:
10.1016/j.algal.2015.02.006

2. Biomass, lipid productivity and fatty acid composition of fresh water microalga Rhopalosolen saccatus cultivated under phosphorous limited conditions

Author:
Challagulla, Vineela; Fabbro, Larelle; Nayar, Sasi
Source:
Algal research 2015 v.8 pp. 69-75
ISSN:
2211-9264
Subject:
Navicula; Nitzschia; algae culture; biomass; culture media; dry season; electrical conductivity; fatty acid composition; fatty acids; feedstocks; freshwater; lipid content; microalgae; pH; phosphorus; surface water; water temperature; Queensland
Abstract:
... This study reports the bioprospecting of microalgae as a potential feedstock for biomass and lipid production in the Central Queensland region, Australia. During the dry season of 2010, ninety water samples were collected from a range of water bodies across the Central Queensland region of Australia. These water bodies had pH ranging from of 5.8–8.8, electrical conductivity of 184–2351μScm−1 and w ...
DOI:
10.1016/j.algal.2015.01.010

3. Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls

Author:
Wei, Yifan; Niu, Jianfeng; Huan, Li; Huang, Aiyou; He, Linwen; Wang, Guangce
Source:
Algal research 2015 v.8 pp. 135-139
ISSN:
2211-9264
Subject:
Chlamydomonas reinhardtii; Chlorella vulgaris; Dunaliella salina; Phaeodactylum tricornutum; cell walls; double-stranded RNA; microalgae; peptides; staining
Abstract:
... Cell penetrating peptides (CPPs) are effective molecular tools that can deliver various exogenous cargoes into different types of cells. However, no study to date has evaluated the use of CPPs to import nucleic acid substances into microalgae, which would be advantageous for numerous research and commercial applications. In this study, we tested the ability of nona-(l)-arginine (R9) to conduct CPP ...
DOI:
10.1016/j.algal.2015.02.002

4. Combined effects of irradiance, temperature and nitrate concentration on phycoerythrin content in the microalga Rhodomonas sp. (Cryptophyceae)

Author:
Chaloub, Ricardo M.; Motta, Nathania Maria S.; de Araujo, Silvia P.; de Aguiar, Paula F.; da Silva, Anita F.
Source:
Algal research 2015 v.8 pp. 89-94
ISSN:
2211-9264
Subject:
Rhodomonas; biomass production; cosmetics; culture media; fluorescent dyes; light intensity; microalgae; natural dyes; nitrates; photons; photoperiod; phycoerythrin; response surface methodology; specific growth rate; temperature
Abstract:
... The marine microalga Rhodomonas sp. was studied as a potential resource for the production of phycoerythrin, a red-colored phycobiliprotein which has been used as a fluorescent probe and analytical reagent, as well as a natural dye in food and cosmetics. Influence of temperature, photon flux density, nitrate concentration and light regime on specific growth rate, biomass productivity and phycoeryt ...
DOI:
10.1016/j.algal.2015.01.008

5. Effect of different light spectra on the growth and productivity of acclimated Nannochloropsis sp. (Eustigmatophyceae)

Author:
Vadiveloo, Ashiwin; Moheimani, Navid R.; Cosgrove, Jeffrey J.; Bahri, Parisa A.; Parlevliet, David
Source:
Algal research 2015 v.8 pp. 121-127
ISSN:
2211-9264
Subject:
Nannochloropsis; algae culture; biomass production; blue light; chlorophyll; economics; electricity; energy; green light; lamps; light intensity; lipid content; microalgae; photons; photosynthetically active radiation; photovoltaic cells; solar radiation; specific growth rate
Abstract:
... There is a great need to further develop new technologies in the field of microalgal cultivation system. The integration of selectively wavelength-permeable photovoltaic cells together with microalgae cultivation systems to improve economics has been previously proposed. With no light supplementation, this means that filtered irradiance incident upon the culture is a portion of the full solar irra ...
DOI:
10.1016/j.algal.2015.02.001

6. Effect of harvesting methods on the reusability of water for cultivation of Chlorella vulgaris, its lipid productivity and biodiesel quality

Author:
Farooq, Wasif; Moon, Myounghoon; Ryu, Byung-gon; Suh, William I.; Shrivastav, Anupama; Park, Min S.; Mishra, Sanjiv K.; Yang, Ji-Won
Source:
Algal research 2015 v.8 pp. 1-7
ISSN:
2211-9264
Subject:
Chlorella vulgaris; algae culture; alum; biodiesel; biomass; biorefining; centrifugation; culture media; ferric chloride; flocculation; harvesting; ions; iron; lipids; microalgae; pH; proteins; recycling; washing; water footprint
Abstract:
... The large water footprint is one of the major bottlenecks for the sustainable microalgae-based biorefinery. In order to reduce the amount of water that is needed for mass cultivation of microalgae, the reusability of culture medium for further algal growth was tested. Chlorella vulgaris was cultivated in recycled medium that was obtained from harvesting microalgal cells by using either centrifugat ...
DOI:
10.1016/j.algal.2014.12.007

7. Effect of light level and salinity on the composition and accumulation of free and ester-type carotenoids in the aerial microalga Scenedesmus sp. (Chlorophyceae)

Author:
Aburai, Nobuhiro; Sumida, Daichi; Abe, Katsuya
Source:
Algal research 2015 v.8 pp. 30-36
ISSN:
2211-9264
Subject:
Scenedesmus; astaxanthin; chlorophyll; developmental stages; esterification; fatty acid esters; light intensity; microalgae; nitrates; salinity; salt stress; sodium chloride; specific growth rate; starvation; xanthophylls
Abstract:
... Composition and accumulation of stress-induced carotenoids were characterized in the aerial microalga Scenedesmus sp. KGU-Y002. The specific growth rate was 0.27d−1, in the logarithmic growth phase. Nitrate starvation and varying light levels were applied to determine the effect of stress on the carotenogenesis of the algal cells. Under 200μmolphotonsm−2s−1, the alga accumulated 34.2±3.8mgg−1drywe ...
DOI:
10.1016/j.algal.2015.01.005

8. First developments towards closing the nutrient cycle in a biofuel production process

Author:
Bagnoud-Velásquez, M.; Schmid-Staiger, U.; Peng, G.; Vogel, F.; Ludwig, Ch.
Source:
Algal research 2015 v.8 pp. 76-82
ISSN:
2211-9264
Subject:
aqueous solutions; biofuels; biomass production; carbon; culture media; fuel production; liquefaction; microalgae; nutrients; recycling; salts; wastes; water management
Abstract:
... Hydrothermal liquefaction (HTL) of microalgae is a promising technology offering production of biofuels in a sustainable way. Thanks to the supercritical conditions applied, the recycling of water and nutrients including carbon capture becomes feasible1–2. Through HTL, the target bio-oil is generated together with process water containing valuable nutrients. In this study we have provided an envir ...
DOI:
10.1016/j.algal.2014.12.012

9. Forward osmosis with waste glycerol for concentrating microalgae slurries

Author:
Mazzuca Sobczuk, T.; Ibáñez González, M.J.; Molina Grima, E.; Chisti, Y.
Source:
Algal research 2015 v.8 pp. 168-173
ISSN:
2211-9264
Subject:
biodiesel; biomass; dewatering; dialysis; energy efficiency; freshwater; fuel production; glycerol; microalgae; osmosis; slurries; wastes
Abstract:
... Forward osmosis of microalgal suspensions was performed by using a dialysis membrane submerged in pure glycerol or a simulated crude glycerol of a biodiesel production process. Freshwater, marine and hypersaline microalgae were concentrated from around 1 to 2.5g·L−1 within an hour of treatment with crude glycerol. Cultures of marine microalgae could be osmotically dehydrated using crude glycerol a ...
DOI:
10.1016/j.algal.2015.02.008

10. High light and carbon dioxide optimize surface productivity in a Twin-Layer biofilm photobioreactor

Author:
Schultze, Larissa K.P.; Simon, Marie-Victoria; Li, Tong; Langenbach, Dorothee; Podola, Björn; Melkonian, Michael
Source:
Algal research 2015 v.8 pp. 37-44
ISSN:
2211-9264
Subject:
Chlorophyta; biofilm; biomass production; carbon dioxide; light intensity; microalgae; models; photobioreactors
Abstract:
... Immobilized cultivation of microalgae recently received increasing attention avoiding some of the major drawbacks of suspension cultivation. Twin-Layer photobioreactors (TL-PBRs), a type of porous substrate bioreactor (PSBR), have proven to be an efficient cultivation system for microalgal biofilms. However, the TL-PBR had not yet been optimized with respect to light and CO2. The effect of light i ...
DOI:
10.1016/j.algal.2015.01.007

11. Impact of heavy metals from flue gas integration with microalgae production

Author:
Napan, Katerine; Teng, Lihong; Quinn, Jason C.; Wood, Byard D.
Source:
Algal research 2015 v.8 pp. 83-88
ISSN:
2211-9264
Subject:
Scenedesmus obliquus; algae culture; arsenic; biodiesel; biomass; cadmium; carbon dioxide; chromium; coal; cobalt; copper; flue gas; fuel production; heavy metals; lead; lipids; mercury; microalgae; nickel; power plants; selenium; toxicity; zinc
Abstract:
... Integration of microalgae cultivation with coal-based flue gas is a widely proposed approach to supply carbon dioxide (CO2). The utilization of coal derived CO2 in microalgae growth systems will introduce heavy metals (toxic metals and metalloids), originally present in coal, into the cultivation system. This study evaluates the effect of 10 heavy metals (As, Cd, Co, Cr, Cu, Hg, Ni, Pb, Se and Zn) ...
DOI:
10.1016/j.algal.2015.01.003

12. Investigation of light/dark cycles effects on the photosynthetic growth of Chlamydomonas reinhardtii in conditions representative of photobioreactor cultivation

Author:
Takache, Hosni; Pruvost, Jérémy; Marec, Hélène
Source:
Algal research 2015 v.8 pp. 192-204
ISSN:
2211-9264
Subject:
Chlamydomonas reinhardtii; biomass; kinetics; long term experiments; microalgae; mixing; models; photobioreactors; photophase; photosynthesis; prediction
Abstract:
... In microalgae cultivation, the combination of light attenuation and culture mixing exposes the microalgae to fluctuating light regimes, which is a factor that can interact with cell metabolism and affect photosynthetic conversion. Here we investigate the effect of light/dark cycles (L/D cycles) on the growth of microalga Chlamydomonas reinhardtii using a photobioreactor equipped with a light-emitt ...
DOI:
10.1016/j.algal.2015.02.009

13. Polyhydroxybutyrate production using a wastewater microalgae based media

Author:
Rahman, Asif; Putman, Ryan J.; Inan, Kadriye; Sal, Fulya Ay; Sathish, Ashik; Smith, Terence; Nielsen, Chad; Sims, Ronald C.; Miller, Charles D.
Source:
Algal research 2015 v.8 pp. 95-98
ISSN:
2211-9264
Subject:
Escherichia coli; algae culture; biofuels; centrifugation; culture media; heavy metals; hydrolysis; lipids; microalgae; nitrogen; phosphorus; polyhydroxybutyrate; value added; wastewater; wastewater treatment
Abstract:
... Bioproduct production from wastewater microalgae has the potential to contribute to societal needs with value added chemicals. Microalgae can remediate wastewater to remove nitrogen, phosphorus, and heavy metals and can be processed to produce biofuels and bioproducts. It was previously demonstrated that recombinant Escherichia coli could produce polyhydroxybutyrates (PHBs) when cultured on a wast ...
DOI:
10.1016/j.algal.2015.01.009

14. Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae Nannochloropsis

Author:
Parniakov, Oleksii; Barba, Francisco J.; Grimi, Nabil; Marchal, Luc; Jubeau, Sébastien; Lebovka, Nikolai; Vorobiev, Eugene
Source:
Algal research 2015 v.8 pp. 128-134
ISSN:
2211-9264
Subject:
Nannochloropsis; antioxidant activity; carbohydrates; microalgae; pH; phenolic compounds; pigments; proteins; pulsed electric fields
Abstract:
... The study was aimed at investigation of the potential of pulsed electric field (PEF) pre-treatment as a preliminary step of pH-assisted aqueous extraction of algae components from microalgae Nannochloropsis suspensions. The PEF and sonication (S) were compared as pretreatment methods. They were applied at normal (pH=8.5) and basic (pH=11) conditions, and supplementary basic extraction (at pH=11) w ...
DOI:
10.1016/j.algal.2015.01.014

15. Study of bioflocculation induced by Saccharomyces bayanus var. uvarum and flocculating protein factors in microalgae

Author:
Díaz-Santos, Encarnación; Vila, Marta; de la Vega, Marta; León, Rosa; Vigara, Javier
Source:
Algal research 2015 v.8 pp. 23-29
ISSN:
2211-9264
Subject:
Chlamydomonas reinhardtii; Saccharomyces bayanus; culture media; flocculation; freshwater; harvesting; lectins; microalgae; models; yeasts
Abstract:
... Autoaggregation of flocculent microalgae in response to stressing conditions is poorly understood, but it is a promising approach to induce the aggregation of microalgae into flocs and make microalgal harvesting a straightforward and cheap procedure. The effect of the self-flocculating yeast strain Saccharomyces bayanus var. uvarum on two chlorophytes: the model freshwater microalga Chlamydomonas ...
DOI:
10.1016/j.algal.2014.12.013

16. Two-stage hydrothermal liquefaction of a high-protein microalga

Author:
Jazrawi, Christopher; Biller, Patrick; He, Yaya; Montoya, Alejandro; Ross, Andrew B.; Maschmeyer, Thomas; Haynes, Brian S.
Source:
Algal research 2015 v.8 pp. 15-22
ISSN:
2211-9264
Subject:
Chlorella; biofuels; biomass; formic acid; liquefaction; microalgae; nitrogen; nitrogen compounds; nitrogen content; oils; refining; sulfuric acid
Abstract:
... Hydrothermal liquefaction (HTL) is a promising route for producing renewable fuels and chemicals from algal biomass. However, the protein fraction of the alga gives rise to nitrogen compounds in the oil fraction, which may render the oil unattractive for use in conventional refining processes. We report a two-stage HTL approach with the primary aim of reducing the nitrogen concentration in the bio ...
DOI:
10.1016/j.algal.2014.12.010

17. Understanding the effect of cell disruption methods on the diffusion of Chlorella vulgaris proteins and pigments in the aqueous phase

Author:
Safi, Carl; Frances, Christine; Ursu, Alina Violeta; Laroche, Céline; Pouzet, Cécile; Vaca-Garcia, Carlos; Pontalier, Pierre-Yves
Source:
Algal research 2015 v.8 pp. 61-68
ISSN:
2211-9264
Subject:
Chlorella vulgaris; chemical elements; diffusivity; homogenization; hydrolysis; microalgae; microscopy; milling; pigments; proteins; ultrasonic treatment
Abstract:
... Cell disruption of microalgae is usually evaluated by microscopic observations and quantification of the target molecules before and after cell disruption. The following study considers a new approach by analysing the diffusion behaviour of proteins and pigments of Chlorella vulgaris in an aqueous medium after applying different cell disruption methods. Results were revealed by microscopic observa ...
DOI:
10.1016/j.algal.2015.01.002

18. Use of methylene blue uptake for assessing cell viability of colony-forming microalgae

Author:
Bicas, Juliano Lemos; Kleinegris, Dorinde M.M.; Barbosa, Maria J.
Source:
Algal research 2015 v.8 pp. 174-180
ISSN:
2211-9264
Subject:
Botryococcus braunii; Ettlia oleoabundans; biofuels; biomass; colorimetry; flow cytometry; fuel production; hydrocarbons; lipids; methylene blue; microalgae; viability
Abstract:
... During the past few years, interest in microalgae has grown, mainly because of their potential for biofuel production. Botryococcus braunii, a green microalga that can accumulate more than half of its dry weight as hydrocarbons, is one of the most important examples. This microorganism grows in colonies and there has been no reliable viability protocol reported for this species as yet. Knowing the ...
DOI:
10.1016/j.algal.2015.02.004