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- Wu, Qing-Ping; Zhang, Lei; Shao, Xiao-Xia; Wang, Jia-Hui; Gao, Yu; Xu, Zeng-Guang; Liu, Ya-Li; Guo, Zhan-Yun
- Amino acids 2016 v.48 no.4 pp. 1099-1107
- G-protein coupled receptors, etc ; crosslinking; screening; Komagataella pastoris; humans; antagonists; ligands; bioluminescence; peptide hormones; relaxin; agonists; binding capacity; cysteine; Show all 13 Subjects
- ... Relaxin is a prototype of the relaxin family peptide hormones and plays important biological functions by binding and activating the G protein-coupled receptor RXFP1. To study their interactions, in the present work, we applied the newly developed bioluminescent ligand–receptor binding assay to the relaxin-RXFP1 system. First, a fully active easily labeled relaxin, in which three Lys residues of h ...
- Patil, Nitin A.; Bathgate, Ross A. D.; Kocan, Martina; Ang, Sheng Yu; Tailhades, Julien; Separovic, Frances; Summers, Roger; Grosse, Johannes; Hughes, Richard A.; Wade, John D.; Hossain, Mohammed Akhter
- Amino acids 2016 v.48 no.4 pp. 987-992
- G-protein coupled receptors, etc ; acids; amides; anorexia; colon; disulfide bonds; drugs; electrostatic interactions; food intake; humans; obesity; peptides; relaxin; Show all 13 Subjects
- ... Insulin-like peptide 5 (INSL5) is an orexigenic peptide hormone belonging to the relaxin family of peptides. It is expressed primarily in the L-cells of the colon and has a postulated key role in regulating food intake. Its G protein-coupled receptor, RXFP4, is a potential drug target for treating obesity and anorexia. We studied the effect of modification of the C-terminus of the A and B-chains o ...