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You searched for: Journal Amino acids Remove constraint Journal: Amino acids Publication Year 2010 Remove constraint Publication Year: 2010 Source 2010 v.38 no.5 Remove constraint Source: 2010 v.38 no.5
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1. Adenosine receptor agonists affect taurine release from mouse brain stem slices in ischemia

Author:
Saransaari, Pirjo; Oja, Simo S.
Source:
Amino acids 2010 v.38 no.5 pp. 1387-1393
ISSN:
0939-4451
Subject:
adenosine; adults; agonists; antagonists; brain stem; ischemia; mice; normoxia; purinergic receptors; taurine; tritium
Abstract:
... The release of the inhibitory amino acid taurine is markedly enhanced under ischemic conditions in both adult and developing brain stem, together with a pronounced increase in the release of the neuromodulator adenosine. We now studied the effects of adenosine receptor agonists and antagonists on [³H]taurine release in the brain stem in normoxia and ischemia, using a superfusion system. Under stan ...
DOI:
10.1007/s00726-009-0351-7
PubMed:
19768520

2. Aqueous-phase quantitative NMR determination of amino acid enantiomer ratio by ¹³C-NMR using chiral neodymium shift reagent

Author:
Florini, Nicola; Faglioni, Francesco; Zucchi, Claudia; Caglioti, Luciano; Pályi, Gyula
Source:
Amino acids 2010 v.38 no.5 pp. 1343-1350
ISSN:
0939-4451
Subject:
amino acids; enantiomers; neodymium; nuclear magnetic resonance spectroscopy; quantitative analysis
Abstract:
... A neodymium-(S)-PDTA (PDTA = N,N,N′,N′-tetrakis[(hydroxycarbonyl)methyl]-1,2-diaminopropane) complex was found exceptionally useful in the quantitative determination of enantiomer ratios of water-soluble natural amino acids by ¹³C-NMR. The method is demonstrated on mixtures of l- and d-enantiomers of various amino acids. The interactions of the chiral shift reagent with the amino acid molecules we ...
DOI:
10.1007/s00726-009-0341-9

3. Changes in plasma amino acid concentrations with increasing age in patients with propionic acidemia

Author:
Scholl-Bürgi, Sabine; Sass, Jörn Oliver; Heinz-Erian, Peter; Amann, Edda; Haberlandt, Edda; Albrecht, Ursula; Ertl, Claudia; Sigl, Sara Baumgartner; Lagler, Florian; Rostasy, Kevin; Karall, Daniela
Source:
Amino acids 2010 v.38 no.5 pp. 1473-1481
ISSN:
0939-4451
Subject:
alanine; arginine; asparagine; aspartic acid; correlation; glutamic acid; glutamine; histidine; isoleucine; leucine; lysine; methionine; normal values; ornithine; patients; phenylalanine; proline; propionyl-CoA carboxylase; serine; threonine; valine
Abstract:
... The objective of the study is to analyze plasma amino acid concentrations in propionic acidemia (PA) for the purpose of elucidating possible correlations between propionyl-CoA carboxylase deficiency and distinct amino acid behavior. Plasma concentrations of 19 amino acids were measured in 240 random samples from 11 patients (6 families) with enzymatically and/or genetically proven propionic acidem ...
DOI:
10.1007/s00726-009-0356-2

4. Chemiluminescence from thermal oxidation of amino acids and proteins

Author:
Millington, Keith R.; Ishii, Hiroshi; Maurdev, George
Source:
Amino acids 2010 v.38 no.5 pp. 1395-1405
ISSN:
0939-4451
Subject:
advanced glycation end products; air; arginine; asparagine; chemiluminescence; fluorescence; heat; lysine; milk proteins; nitrogen; oxidation; polyamides; schiff bases; singlet oxygen; threonine; tryptophan
Abstract:
... Chemiluminescence (CL) with maximum emission in the range 550-650 nm is observed when proteins and certain amino acids are heated in air, and CL intensity is significantly reduced in nitrogen. Of the 20 common amino acids, lysine (Lys) has the highest thermal CL intensity by a factor of ~30 over arginine, threonine and asparagine. This finding differs from previous studies on amino acids and prote ...
DOI:
10.1007/s00726-009-0352-6
PubMed:
19763784

5. Chronic hyperhomocysteinemia impairs vascular function in ovariectomized rat carotid arteries

Author:
Celotto, Andréa Carla; Fukada, Sandra Y.; Laurindo, Francisco R. M.; Haddad, Renato; Eberlin, Marcos N.; de Oliveira, Ana Maria
Source:
Amino acids 2010 v.38 no.5 pp. 1515-1522
ISSN:
0939-4451
Subject:
acetylcholine; adrenergic agonists; body weight; carotid arteries; coronary disease; endothelial nitric oxide synthase; females; homocysteine; inducible nitric oxide synthase; nitric oxide; nitroprusside; ovariectomy; rats; risk factors; vasoconstriction
Abstract:
... Homocysteine is an independent risk factor for coronary heart disease, as well as for cerebrovascular and peripheral vascular diseases. The purpose of this study was to investigate the effects of hyperhomocysteinemia (HHcy) on vascular reactivity within carotid artery segments isolated from ovariectomized female rats. Treatment with dl-Hcy thiolactone (1 g/kg body weight per day) reduced the pheny ...
DOI:
10.1007/s00726-009-0368-y
PubMed:
19876715

6. Daily quadratic trend in basal monocyte expressed HSP72 in healthy human subjects

Author:
Taylor, Lee; Midgley, Adrian W.; Chrismas, Bryna; Madden, Leigh A.; Vince, Rebecca V.; McNaughton, Lars R.
Source:
Amino acids 2010 v.38 no.5 pp. 1483-1488
ISSN:
0939-4451
Subject:
blood serum; circadian rhythm; diurnal variation; heat shock proteins; humans; males; monocytes; temperature
Abstract:
... The inducible human stress protein heat shock protein 72 (HSP72) performs vital roles within the body at rest and during periods of stress. Recently it was shown over a 24 h period that basal HSP72 followed a diurnal variation. However, these results and previous literature demonstrate noticeable inter-subject variation in basal HSP72 expression. The notion of intra/inter-day variation in basal HS ...
DOI:
10.1007/s00726-009-0360-6
PubMed:
19816654

7. Detection of d-amino acids in purified proteins synthesized in Escherichia coli

Author:
Miyamoto, Tetsuya; Sekine, Masae; Ogawa, Tetsuhiro; Hidaka, Makoto; Homma, Hiroshi; Masaki, Haruhiko
Source:
Amino acids 2010 v.38 no.5 pp. 1377-1385
ISSN:
0939-4451
Subject:
Escherichia coli; acid hydrolysis; amino acids; beta-galactosidase; humans; hydrolysates; linear models; molecular weight; protein synthesis; proteins; tissues
Abstract:
... It has long been believed that amino acids comprising proteins of all living organisms are only of the l-configuration, except for Gly. However, peptidyl d-amino acids were observed in hydrolysates of soluble high molecular weight fractions extracted from cells or tissues of various organisms. This strongly suggests that significant amounts of d-amino acids are naturally present in usual proteins. ...
DOI:
10.1007/s00726-009-0348-2
PubMed:
19768521

8. Distinct anabolic signalling responses to amino acids in C2C12 skeletal muscle cells

Author:
Atherton, Philip J.; Smith, Ken; Etheridge, Timothy; Rankin, Debbie; Rennie, Michael J.
Source:
Amino acids 2010 v.38 no.5 pp. 1533-1539
ISSN:
0939-4451
Subject:
blood serum; chemical structure; essential amino acids; immunoblotting; isoleucine; leucine; mammals; myotubes; non-specific serine/threonine protein kinase; phosphorylation; protein synthesis; signal transduction; skeletal muscle; valine
Abstract:
... The essential amino acids (EAA) activate anabolic signalling through mechanisms, which are unclear in detail but include increased signalling through the mammalian target of rapamycin complex 1 (mTORC1). Of all the EAA, the branched chain amino acid (BCAA) leucine has been suggested as the most potent in stimulating protein synthesis, although there have been no studies investigating the effects o ...
DOI:
10.1007/s00726-009-0377-x
PubMed:
19882215

9. Effects of zinc ex vivo and intracellular zinc chelator in vivo on taurine uptake in goldfish retina

Author:
Nusetti, S.; Urbina, M.; Obregón, F.; Quintal, M.; Benzo, Z.; Lima, L.
Source:
Amino acids 2010 v.38 no.5 pp. 1429-1437
ISSN:
0939-4451
Subject:
chelating agents; goldfish; high performance liquid chromatography; nutrient deficiencies; retina; spectroscopy; taurine; transporters; tritium; zinc; zinc sulfate
Abstract:
... Taurine and zinc exert neurotrophic effects. Zinc modulates Na⁺/Cl⁻-dependent transporters. This study examined the effect of zinc (ZnSO₄) ex vivo and zinc chelator N,N,N′,N′-tetrakis-(2-pyridylmethyl) ethylenediamine (TPEN) in vivo on [³H]taurine transport in goldfish retina. The effect of TPEN in vivo on taurine and zinc levels was determined. Isolated cells were incubated in Ringer with zinc (0 ...
DOI:
10.1007/s00726-009-0357-1
PubMed:
19784807

10. Enriching the viral-host interactomes with interactions mediated by SH3 domains

Author:
Carducci, Martina; Licata, Luana; Peluso, Daniele; Castagnoli, Luisa; Cesareni, Gianni
Source:
Amino acids 2010 v.38 no.5 pp. 1541-1547
ISSN:
0939-4451
Subject:
protein binding; Human papillomavirus type 16; proteome; oncogenic viruses; humans; peptides; Human mastadenovirus B; protein-protein interactions; databases
Abstract:
... Protein-protein interactions play an essential role in the regulation of most cellular processes. The process of viral infection is no exception and many viral pathogenic strategies involve targeting and perturbing host-protein interactions. The characterization of the host protein subnetworks disturbed by invading viruses is a major goal of viral research and may contribute to reveal fundamental ...
DOI:
10.1007/s00726-009-0375-z

11. Evidence for the involvement of d-aspartic acid in learning and memory of rat

Author:
Topo, Enza; Soricelli, Andrea; Di Maio, Angela; D'Aniello, Enrico; Di Fiore, Maria Maddalena; D'Aniello, Antimo
Source:
Amino acids 2010 v.38 no.5 pp. 1561-1569
ISSN:
0939-4451
Subject:
amino acids; analysis of variance; cognition; correlation; hippocampus; memory; oral administration; rats; sodium
Abstract:
... d-Aspartic acid (d-Asp) is an endogenous amino acid present in neuroendocrine systems. Here, we report evidence that d-Asp in the rat is involved in learning and memory processes. Oral administration of sodium d-aspartate (40 mM) for 12-16 days improved the rats' cognitive capability to find a hidden platform in the Morris water maze system. Two sessions per day for three consecutive days were per ...
DOI:
10.1007/s00726-009-0369-x
PubMed:
19890700

12. Folding properties of the hepatitis B core as a carrier protein for vaccination research

Author:
Janssens, Michiel Etienne; Geysen, Dirk; Broos, Katleen; De Goeyse, Ine; Robbens, Johan; Van Petegem, Filip; Timmermans, Jean-Pierre; Guisez, Yves
Source:
Amino acids 2010 v.38 no.5 pp. 1617-1626
ISSN:
0939-4451
Subject:
capsid; chimerism; disulfide bonds; glycine (amino acid); hepatitis B; peptides; prediction; recombinant fusion proteins; vaccination
Abstract:
... The hepatitis B core (HBc) protein has been used successfully in numerous experiments as a carrier for heterologous peptides. Folding and capsid formation of the chimeric proteins is not always achieved easily. In silico analyses were performed to provide further comprehension of the feasibility for predicting successful capsid formation. In contrast to previous work, we show that common in silico ...
DOI:
10.1007/s00726-009-0365-1
PubMed:
19957000

13. Free amino acid production during tomato fruit ripening: a focus on l-glutamate

Author:
Sorrequieta, Augusto; Ferraro, Gisela; Boggio, Silvana B.; Valle, Estela M.
Source:
Amino acids 2010 v.38 no.5 pp. 1523-1532
ISSN:
0939-4451
Subject:
alanine; aspartate transaminase; cultivars; free amino acids; fruits; glutamate dehydrogenase; glutamic acid; glutathione; metabolism; peptidases; polypeptides; proteins; ripening; tomatoes
Abstract:
... In tomato, free amino acids increase dramatically during fruit ripening and their abundance changed differentially. More evident is l-glutamate which gives the characteristic “umami” flavor. Glutamate is the principal free amino acid of ripe fruits of cultivated varieties. In this paper, we examined the capacity of tomato fruits to process endogenous as well as exogenous polypeptides during the ri ...
DOI:
10.1007/s00726-009-0373-1
PubMed:
19876714

14. Icaritin induces growth inhibition and apoptosis of human prostatic smooth muscle cells in an estrogen receptor-independent manner

Author:
Chen, Min-Feng; Qi, Lin; Li, Yuan; Zu, Xiong-Bing; Dai, Yuan-Qin; Zhang, Peng
Source:
Amino acids 2010 v.38 no.5 pp. 1505-1513
ISSN:
0939-4451
Subject:
Western blotting; antagonists; apoptosis; cell growth; cyclins; estrogen receptors; estrogens; growth retardation; humans; hyperplasia; mitogen-activated protein kinase; myocytes; phosphorylation; signal transduction; smooth muscle
Abstract:
... Icaritin has selective estrogen receptor (ER) modulating activity. ERs are expressed in the prostate stroma, and estrogens have an important role in the pathology of benign prostatic hyperplasia (BPH). However, the impact of icaritin on BPH was not studied. Human prostatic smooth muscle cells (PSMCs) were treated with 0-100 μM icaritin, also using 10 μM ICI182780 as a specific ER antagonist. The e ...
DOI:
10.1007/s00726-009-0366-0
PubMed:
19876716

15. Imaging of human glioma cells by means of a Syndecan-4 directed DOTA-conjugate

Author:
Sturzu, Alexander; Kalbacher, Hubert; Echner, Hartmut; Klose, Uwe; Gharabaghi, Alireza; Heckl, Stefan
Source:
Amino acids 2010 v.38 no.5 pp. 1415-1421
ISSN:
0939-4451
Subject:
amino acids; antibodies; brain; confocal laser scanning microscopy; cytoplasm; cytotoxicity; flow cytometry; fluorescent dyes; gadolinium; glycoproteins; humans; image analysis; magnetic resonance imaging; neoplasms; radiolabeling
Abstract:
... The extracellular glycoprotein Tenascin-C (TN-C) is highly upregulated in gliomas. Therefore, many chemotherapies with radiolabeled antibodies against TN-C have been performed. However, TN-Cs binding partner Syndecan-4 did not play any role as a therapeutic or imaging target in gliomas. We constructed an imaging compound containing the magnetic resonance imaging (MRI) contrast agent gadolinium (Gd ...
DOI:
10.1007/s00726-009-0345-5

16. Induction of type I interferon by RNA viruses: cellular receptors and their substrates

Author:
Baum, Alina; García-Sastre, Adolfo
Source:
Amino acids 2010 v.38 no.5 pp. 1283-1299
ISSN:
0939-4451
Subject:
RNA; RNA viruses; Toll-like receptors; adaptive immunity; genes; innate immunity; interferons; melanoma; retinoic acid; virus replication; viruses
Abstract:
... Virus recognition and induction of interferon (IFN) are critical components of the innate immune system. The Toll-like receptor (TLR) and RIG-I-like receptor families have been characterized as key players in RNA virus detection. Signaling cascades initiated by these receptors are crucial for establishment of an IFN signaling mediated antiviral state in infected and neighboring cells and containme ...
DOI:
10.1007/s00726-009-0374-0
PubMed:
19882216
PubMed Central:
PMC2860555

17. In vitro heat shock of human monocytes results in a proportional increase of inducible Hsp70 expression according to the basal content

Author:
Vince, Rebecca V.; Oliver, Katherine; Midgley, Adrian W.; McNaughton, Lars R.; Madden, Leigh A.
Source:
Amino acids 2010 v.38 no.5 pp. 1423-1428
ISSN:
0939-4451
Subject:
centrifugation; circadian rhythm; heat shock response; heat stress; heat-shock protein 70; humans; males; monocytes; temperature; volunteers
Abstract:
... Heat shock proteins play an important role as molecular chaperones of the cell. Inducible heat shock protein 70 is rapidly synthesised in response to numerous stressors and monocytes are sensitive to changes in core temperature resulting in a circadian variation of Hsp70 expression. Monocytes were isolated via density centrifugation from nine healthy male volunteers at 5 am, 1 pm and 9 pm, represe ...
DOI:
10.1007/s00726-009-0354-4
PubMed:
19779802

18. In vivo biosynthesis of an Ala-scan library based on the cyclic peptide SFTI-1

Author:
Austin, Jeffrey; Kimura, Richard H.; Woo, Youn-Hi; Camarero, Julio A.
Source:
Amino acids 2010 v.38 no.5 pp. 1313-1322
ISSN:
0939-4451
Subject:
Escherichia coli; Helianthus annuus; biosynthesis; directed evolution; disulfide bonds; mutants; peptide libraries; polypeptides; screening; trypsin inhibitors
Abstract:
... We present the in vivo biosynthesis of wild-type sunflower trypsin inhibitor 1 (SFTI-1) inside E. coli cells using an intramolecular native chemical ligation in combination with a modified protein splicing unit. SFTI-1 is a small backbone cyclized polypeptide with a single disulfide bridge. A small library containing multiple Ala mutants was also biosynthesized and its activity was assayed using a ...
DOI:
10.1007/s00726-009-0338-4
PubMed:
19685144
PubMed Central:
PMC2936557

19. Isoform-specific determinants in the HP1 binding to histone 3: insights from molecular simulations

Author:
Machado, Matias R.; Dans, Pablo D.; Pantano, Sergio
Source:
Amino acids 2010 v.38 no.5 pp. 1571-1581
ISSN:
0939-4451
Subject:
Human immunodeficiency virus 1; electrostatic interactions; epigenetics; genome; heterochromatin; histones; methylation; phosphorylation
Abstract:
... Despite the significant improvements in anti HIV-1 treatment, AIDS remains a lifelong disease due to the impossibility to eradicate the viral reservoir established upon integration of the viral genome. Controlling the epigenetic block imposed by the host cell machinery to the viral transcription may represent a therapeutic alternative to purge the viral reservoir, offering a way to eradicate the i ...
DOI:
10.1007/s00726-009-0371-3
PubMed:
19888549

20. Medium optimization for production of gamma-aminobutyric acid by Lactobacillus brevis NCL912

Author:
Li, Haixing; Qiu, Ting; Gao, Dandan; Cao, Yusheng
Source:
Amino acids 2010 v.38 no.5 pp. 1439-1445
ISSN:
0939-4451
Subject:
Lactobacillus brevis; gamma-aminobutyric acid; glucose; models; peptones; polysorbates; response surface methodology
Abstract:
... Production of gamma-aminobutyric acid (GABA) was carried out in Erlenmeyer flasks by Lactobacillus brevis NCL912. Traditional methods were first adopted to select the key factors that impact the GABA production to preliminarily determine the suitable concentration ranges of the key factors. It was found that glucose, soya peptone, Tween-80 and MnSO₄·4H₂O were the key factors affecting GABA product ...
DOI:
10.1007/s00726-009-0355-3
PubMed:
19787432