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- Author:
- Pietzsch, J.; Bergmann, R.
- Source:
- Amino acids 2004 v.26 no.1 pp. 45-51
- ISSN:
- 0939-4451
- Subject:
- amino acids; atherogenesis; copper; esters; gas chromatography; iron; low density lipoprotein; mass spectrometry; oxidation; pyridines
- Abstract:
- ... An alteration of low density lipoprotein (LDL) apolipoprotein (apo) B-100 structure by direct oxidative modification is an important mechanism involved in atherogenesis. There is difficulty in quantifying this type of modification because a lack of specific assays. The use of N(O,S)-ethoxycarbonyl trifluoroethyl amino acid esters for a rapid and sensitive determination of 6-hydroxy-2-aminocaproic ...
- DOI:
- 10.1007/s00726-003-0036-6
-
http://dx.doi.org/10.1007/s00726-003-0036-6
- Author:
- Saransaari, P.; Oja, S. S.
- Source:
- Amino acids 2004 v.26 no.1 pp. 91-98
- ISSN:
- 0939-4451
- Subject:
- adenosine; adults; agonists; antagonists; cell membranes; glutamic acid; hippocampus; hydrogen peroxide; hydroxylamine; ischemia; mice; neuroprotective effect; nitric oxide; receptors; taurine; transporters; tritium
- Abstract:
- ... The release of the inhibitory neuromodulator taurine in the hippocampus is markedly enhanced under various neural cell-damaging conditions, including ischemia and exposure to free radicals. The properties and regulation of the release evoked by a medium containing free radicals was investigated in hippocampal slices from adult (3-month-old) and developing (7-day-old) mice, using a superfusion syst ...
- DOI:
- 10.1007/s00726-003-0004-1
-
http://dx.doi.org/10.1007/s00726-003-0004-1
- Author:
- Zhang, M.; Bi, L. F.; Ai, Y. D.; Yang, L. P.; Wang, H. B.; Liu, Z. Y.; Sekine, M.; Kagamimori, S.
- Source:
- Amino acids 2004 v.26 no.1 pp. 59-63
- ISSN:
- 0939-4451
- Subject:
- college students; evoked potentials; males; placebos; taurine; volunteers
- Abstract:
- ... In order to evaluate the effects of dietary taurine supplementation on visual fatigue induced by visual display terminals (VDT) work, 25 male college students aged from 20 to 24 years who were not engaged in VDT work were selected to participate in the study. Volunteers were randomly assigned to either the taurine supplementation (n=13) or the placebo supplementation control group (n=12). Before a ...
- DOI:
- 10.1007/s00726-003-0032-x
-
http://dx.doi.org/10.1007/s00726-003-0032-x
- Author:
- Stipanuk, M. H.; Londono, M.; Hirschberger, L. L.; Hickey, C.; Thiel, D. J.; Wang, L.
- Source:
- Amino acids 2004 v.26 no.1 pp. 99-106
- ISSN:
- 0939-4451
- Subject:
- anion exchange; antibodies; brain; cysteine; dimerization; genes; kidneys; liver; mass spectrometry; molecular weight; polyacrylamide; polyacrylamide gel electrophoresis; post-translational modification; rabbits; rats
- Abstract:
- ... Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration. Because multiple forms of CDO (∼23 kDa, ∼25 kDa, and ∼68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting (with antibodies demonstrated to immunoprecipitate CDO), we further investigated the possibility of more than one CDO isoform. Using either rabbit an ...
- DOI:
- 10.1007/s00726-003-0001-4
-
http://dx.doi.org/10.1007/s00726-003-0001-4
- Author:
- Oh, J. E.; Krapfenbauer, K.; Fountoulakis, M.; Frischer, Th.; Lubec, G.
- Source:
- Amino acids 2004 v.26 no.1 pp. 9-18
- ISSN:
- 0939-4451
- Subject:
- amnion; antibodies; databases; digestion; fibroblasts; gel electrophoresis; genes; human cell lines; humans; kidneys; mass spectrometry; nucleotide sequences; proteins; proteome; transcription (genetics); translation (genetics)
- Abstract:
- ... The human genome maybe limited to about 30 000 genes whereas the human proteome may be represented by a rough estimate of one million proteins. A legion of proteins have been described and information about these structures are readily available in data banks. There remains, however, a large series of unknown or hypothetical proteins (HPs). Many of them have been predicted from nucleic acid sequen ...
- DOI:
- 10.1007/s00726-003-0060-6
-
http://dx.doi.org/10.1007/s00726-003-0060-6
- Author:
- Fountoulakis, M.; Juranville, J.-F.; Tsangaris, G.; Suter, L.
- Source:
- Amino acids 2004 v.26 no.1 pp. 27-36
- ISSN:
- 0939-4451
- Subject:
- desorption; drugs; fractionation; gels; hydrophilicity; ionization; isoelectric focusing; lithium; liver; proteins; proteomics; rats; sulfates; toxicity; toxicity testing; two-dimensional gel electrophoresis
- Abstract:
- ... Proteomics offers unique possibilities to investigate changes in the levels and modifications of proteins involved in the pathomechanisms of diseases and toxic events. However, search for potential drug targets and disease or toxicity markers is limited by the fact that mainly the high-abundance, hydrophilic proteins are visualized in two-dimensional gels. Here we studied the enrichment of rat liv ...
- DOI:
- 10.1007/s00726-003-0041-9
-
http://dx.doi.org/10.1007/s00726-003-0041-9
- Author:
- Peñafiel, R.; Ruzafa, C.; Monserrat, F.; Cremades, A.
- Source:
- Amino acids 2004 v.26 no.1 pp. 53-58
- ISSN:
- 0939-4451
- Subject:
- adults; anserine; carnosine; females; legs; lysine; males; mice; muscles; sexual dimorphism; skeletal muscle; testosterone
- Abstract:
- ... The aminoacyl-imidazole dipeptides carnosine (β-alanyl-L-histidine) and anserine (β-alanyl-1-methyl-histidine) are present in relatively high concentrations in excitable tissues, such as muscle and nervous tissue. In the present study we describe the existence of a marked sexual dimorphism of carnosine and anserine in skeletal muscles of CD1 mice. In adult animals the concentrations of anserine we ...
- DOI:
- 10.1007/s00726-003-0034-8
-
http://dx.doi.org/10.1007/s00726-003-0034-8
- Author:
- Merlo, F.; Balduzzi, R.; Cupello, A.; Robello, M.
- Source:
- Amino acids 2004 v.26 no.1 pp. 77-84
- ISSN:
- 0939-4451
- Subject:
- cerebellum; cytoplasm; fluorescence microscopy; neurites; plasma membrane; rats
- Abstract:
- ... An immunocytochemical investigation of the expression of α₁, α₆, β₂/₃, γ₂and δ subunits was performed on rat cerebellum granule cells in culture by the two photon microscopy technique.The first four subunits appear to be expressed abundantly in these cells, whereas the δ one seems to be expressed at a lower level. Another major difference in the distribution of these subunits is that whereas α₆, β ...
- DOI:
- 10.1007/s00726-003-0006-z
-
http://dx.doi.org/10.1007/s00726-003-0006-z
- Author:
- Bendsoe, N.; Ronquist, G.
- Source:
- Amino acids 2004 v.26 no.1 pp. 19-26
- ISSN:
- 0939-4451
- Subject:
- L-dopa; Langerhans cells; biopsy; energy; humans; plasma membrane; protons; stomach; ultrastructure
- Abstract:
- ... L-3,4-dihydroxyphenylalanine (L-dopa) transport into human Langerhans cells (LC) occurs by a saturable mediation. This plasma membrane agency is, due to its characteristics, distinguishable from systems transporting other neutral, cationic and anionic amino acids into other cells and serves to catalyze the flow of L-dopa, only, into LC. The uphill operation of this L-dopa transport system is belie ...
- DOI:
- 10.1007/s00726-003-0053-5
-
http://dx.doi.org/10.1007/s00726-003-0053-5
- Author:
- Kamiński, R. M.; Żółkowska, D.; Kozicka, M.; Kleinrok, Z.; Czuczwar, S. J.
- Source:
- Amino acids 2004 v.26 no.1 pp. 85-89
- ISSN:
- 0939-4451
- Subject:
- histamine; histidine; isomers; laboratory animals; memory; mice; pharmacokinetics; phenytoin; protective effect; seizures
- Abstract:
- ... Endogenous histamine has been reported to be involved in regulation of seizure susceptibility. Enhancement of histamine neurotransmission engendered by L-histidine treatment produces anticonvulsant effects in experimental animals. The present study investigated the influence of L-histidine on the protective effects of carbamazepine and phenytoin against maximal electroshock-induced seizures in mic ...
- DOI:
- 10.1007/s00726-003-0005-0
-
http://dx.doi.org/10.1007/s00726-003-0005-0
- Author:
- Grillo, M. A.; Colombatto, S.
- Source:
- Amino acids 2004 v.26 no.1 pp. 3-8
- ISSN:
- 0939-4451
- Subject:
- agmatine; animal tissues; arginine; decarboxylation; metabolism; nitric oxide; polyamines
- Abstract:
- ... Recently agmatine, decarboxylated arginine, has been shown to be an important biological compound in several animal tissues. This paper summarizes the known information regarding the transport of arginine, its decarboxylation and the effects of the agmatine formed mainly on NO and polyamine synthesis. ...
- DOI:
- 10.1007/s00726-003-0030-z
-
http://dx.doi.org/10.1007/s00726-003-0030-z
- Author:
- Modi, P.; Suleiman, M.-S.
- Source:
- Amino acids 2004 v.26 no.1 pp. 65-70
- ISSN:
- 0939-4451
- Subject:
- children; infants; ischemia; myocardium; patients; rats; taurine
- Abstract:
- ... Depleting intracellular taurine in heart cells improves their resistance to ischemia and reperfusion injury. The aim of this work was to see whether physiologically low levels of endogenous taurine also reflect a reduced vulnerability of the myocardium to cardiac insults. The myocardial concentration of taurine was measured during different stages of development and compared with vulnerability to ...
- DOI:
- 10.1007/s00726-003-0031-y
-
http://dx.doi.org/10.1007/s00726-003-0031-y
- Author:
- Engelmann, Mario; Wolf, G.; Putzke, J; Bloom, F. E.; Raber, J.; Landgraf, R.; Spina, M. G.; Horn, T. F. W.
- Source:
- Amino acids 2004 v.26 no.1 pp. 37-43
- ISSN:
- 0939-4451
- Subject:
- blood; blood flow; citrulline; hypothalamus; in situ hybridization; messenger RNA; microdialysis; neuronal nitric oxide synthase; neurons; nitric oxide; nitrites; oxytocin; rats; secretion; swimming; vasopressin
- Abstract:
- ... Neurons of the hypothalamo-neurohypophyseal system (HNS) are known to contain high amounts of neuronal nitric oxide (NO) synthase (nNOS). NO produced by those neurons is commonly supposed to be involved as modulator in the release of the two nonapeptides vasopressin (AVP) and oxytocin into the blood stream. Previous studies showed that forced swimming fails to increase the release of AVP into the ...
- DOI:
- 10.1007/s00726-003-0040-x
-
http://dx.doi.org/10.1007/s00726-003-0040-x
- Author:
- van den Baar, M. T. M.; Fekkes, D.; van den Hoogenband, C. R.; Duivenvoorden, H. J.; Pepplinkhuizen, L.
- Source:
- Amino acids 2004 v.26 no.1 pp. 71-76
- ISSN:
- 0939-4451
- Subject:
- glycine (amino acid); lysine; ornithine; sports; taurine; tryptophan; tyrosine
- Abstract:
- ... The aim of this study was to explore the relationship between changes in plasma amino acids and the incidence of sports injuries during a soccer season. Fourteen plasma amino acids were assayed at monthly intervals in 12 young soccer players during a whole soccer season. Based on the number and severity of injuries the soccer players were divided into an injury-prone and a non-injury-prone group. ...
- DOI:
- 10.1007/s00726-003-0008-x
-
http://dx.doi.org/10.1007/s00726-003-0008-x