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- Bai, Yalong; Song, Minghui; Cui, Yan; Shi, Chunlei; Wang, Dapeng; Paoli, George C.; Shi, Xianming
- Analytica chimica acta 2013 v.787 pp. 93
- Listeria monocytogenes, etc ; DNA; Salmonella enteritidis; bacterial contamination; food contamination; food matrix; food pathogens; magnetic separation; microbial detection; nanoparticles; pH; polymerase chain reaction; rapid methods; raw milk; temperature; Show all 15 Subjects
- ... A method based on amino-modified silica-coated magnetic nanoparticles (ASMNPs) and polymerase chain reaction (PCR) was developed to rapidly and sensitively detect foodborne pathogens in raw milk. After optimizing parameters such as pH, temperature, and time, a trace amount of genomic DNA of pathogens could be extracted directly from complex matrices such as raw milk using ASMNPs. The magnetically ...