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- Kim, Chan-Jin; Lee, Dong-Ik; Lee, Chang-Ha; Ahn, Ik-Sung
- Analytica chimica acta 2012 v.723 pp. 101-107
- aspergillopepsin, etc ; binding sites; carboxypeptidase A; chymopapain; chymotrypsin; collagenase; fluorescence; hydrolysis; isoniazid; papain; pepsin; peptidase K; subtilisin; trypsin; Show all 14 Subjects
- ... N,N′-diBoc-dityrosine (DBDY), which was synthesized by the oxidative C–C coupling of 2 N-Boc-l-tyrosine molecules, was conjugated with two isoniazid (INH) molecules. Due to the quenching effect of INH, DBDY–(INH)₂ lacks the fluorescence of DBDY. As such, it was tested for use in the detection of proteases by measuring fluorescence recovery. In this study, serine proteases (chymotrypsin, trypsin, s ...