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- Michael R. Bukowski; Christopher Bucklin; Matthew J. Picklo
- Analytical Biochemistry 2015 v.469 pp. 54-64
- chemical reduction; glutathione; ions; liquid chromatography; liver; mass spectrometry; post-translational modification; proteins; rats
- ... Protein S-glutathionylation is a posttranslational modification that links oxidative stimuli to reversible changes in cellular function. Protein–glutathione mixed disulfide (PSSG) is commonly quantified by reduction of the disulfide and detection of the resultant glutathione species. This methodology is susceptible to contamination by free unreacted cellular glutathione (GSH) species, which are pr ...