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- Baba, Yuji; Yamamoto, Kaho; Yoshida, Wataru
- Analytical and bioanalytical chemistry 2019 v.411 no.19 pp. 4765-4773
- bioluminescence, etc ; DNA; DNA methylation; dyes; energy transfer; luciferase; neoplasms; tumor suppressor genes; Show all 8 Subjects
- ... Abnormal DNA methylations such as hypermethylation on tumor suppressor genes and global hypomethylation have been recognized as hallmarks of cancer. Previously, we reported a bioluminescence resonance energy transfer (BRET)-based global DNA methylation level assay using a methyl-CpG-binding domain-fused firefly luciferase (MBD-Fluc) and unmethylated CpG-binding domain-fused firefly luciferase (CXX ...
- Lopreside, Antonia; Calabretta, Maria Maddalena; Montali, Laura; Ferri, Maura; Tassoni, Annalisa; Branchini, Bruce R.; Southworth, Tara; D’Elia, Marcello; Roda, Aldo; Michelini, Elisa
- Analytical and bioanalytical chemistry 2019 v.411 no.19 pp. 4937-4949
- bioluminescence, etc ; bioavailability; biosensors; cytotoxicity; endocrine-disrupting chemicals; estrogen receptors; estrogenic properties; estrogens; humans; luciferase; plant estrogens; reporter genes; screening; transcription (genetics); translation (genetics); viability; yeasts; Show all 17 Subjects
- ... Cell-based assays utilizing reporter gene technology have been widely exploited for biosensing, as they provide useful information about the bioavailability and cell toxicity of target analytes. The long assay time due to gene transcription and translation is one of the main drawbacks of cell biosensors. We report the development of two yeast biosensors stably expressing human estrogen receptors α ...