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- Xie, Sanlei; Wen, Kai; Xie, Jie; Zheng, Yongjun; Peng, Tao; Wang, Jianyi; Yao, Kai; Ding, Shuangyang; Jiang, Haiyang
- Analytical and bioanalytical chemistry 2018 v.410 no.24 pp. 6197-6205
- antigen-antibody complex, etc ; Escherichia coli; antibodies; chickens; cross reaction; detection limit; drugs; enzyme-linked immunosorbent assay; food safety; horseradish; magnetic materials; magnetism; peroxidase; rapid methods; safety assessment; screening; Show all 16 Subjects
- ... A sensitive competitive immunoassay with simple operation was developed for the detection of the anti-virus drug amantadine (AMD). The single-chain variable fragment (scFv) antibody against AMD was site-specific biotinylated and overexpressed as a secreted body in Escherichia coli AVB101. Horseradish peroxidase-labeled streptavidin-biotinylated scFv antibody (HRP-SA-BIO-scFv) could specifically bi ...