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- Dong, Sa; Zhang, Xiao; Liu, Yuan; Zhang, Cunzheng; Xie, Yajing; Zhong, Jianfeng; Xu, Chongxin; Liu, Xianjin
- Analytical and bioanalytical chemistry 2017 v.409 no.8 pp. 1985-1994
- rice flour, etc ; Bacillus thuringiensis; Oryza sativa; amino acid sequences; animals; cross reaction; crystal proteins; dissociation; endotoxins; enzyme-linked immunosorbent assay; genetically modified plants; haptens; immunization; insecticidal proteins; keyhole limpet hemocyanin; leaves; mastication; monitoring; monoclonal antibodies; pests; polypeptides; rice; Show all 22 Subjects
- ... Cry1Ab toxin is commonly expressed in genetically modified crops in order to control chewing pests. At present, the detection method with enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody cannot specifically detect Cry1Ab toxin for Cry1Ab’s amino acid sequence and spatial structure are highly similar to Cry1Ac toxin. In this study, based on molecular design, a novel hapten pol ...