U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Analytical biochemistry Remove constraint Journal name: Analytical biochemistry Publication year rev 7984-2016 Remove constraint Publication year rev: 7984-2016 Source 2016 v.510 Remove constraint Source: 2016 v.510
Start Over

Search

4  Search Results

1 - 4 of 4
Number of results to display per page

Search Results

1. Electrochemical determination of folic acid: A short review

Author:
S. Akbar; A. Anwar; Q. Kanwal
Source:
Analytical biochemistry 2016 v.510 pp. 98-105
ISSN:
0003-2697
Subject:
DNA; analytical methods; cost effectiveness; electrochemistry; electrodes; folic acid; neural tube defects; risk
Abstract:
... Folic acid (FA) is an electroactive compound of biological origin. It helps our body to produce and maintain healthy cells. It can significantly reduce the occurrence of neural tube defects and also prevents change in DNA structure. FA deficiency can lead to various health risks. Therefore, a sensitive, specific, and reproducible way of FA detection is essential. A number of analytical methods are ...
DOI:
10.1016/j.ab.2016.07.002

2. A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch

Author:
Momoko Takahashi; Ken Akamatsu; Naoya Shikazono
Source:
Analytical biochemistry 2016 v.510 pp. 129-135
ISSN:
0003-2697
Subject:
DNA damage; enzymatic treatment; ionizing radiation; mutagenicity; mutagens; plasmids; progeny
Abstract:
... Exposure of biological materials to ionizing radiation often induces clustered DNA damage. The mutagenicity of clustered DNA damage can be analyzed with plasmids carrying a clustered DNA damage site, in which the strand bias of a replicating plasmid (i.e., the degree to which each of the two strands of the plasmid are used as the template for replication of the plasmid) can help to clarify how clu ...
DOI:
10.1016/j.ab.2016.07.007

3. β-Glucuronidase-coupled assays of glucuronoyl esterases

Author:
Lucia Fraňová; Vladimír Puchart; Peter Biely
Source:
Analytical biochemistry 2016 v.510 pp. 114-119
ISSN:
0003-2697
Subject:
alcohols; beta-glucuronidase; cell walls; esterases; esters; genomic libraries; lignin; microorganisms; screening
Abstract:
... Glucuronoyl esterases (GEs) are microbial enzymes with potential to cleave the ester bonds between lignin alcohols and xylan-bound 4-O-methyl-d-glucuronic acid in plant cell walls. This activity renders GEs attractive research targets for biotechnological applications. One of the factors impeding the progress in GE research is the lack of suitable substrates. In this work, we report a facile prepa ...
DOI:
10.1016/j.ab.2016.07.023

4. Optimization of high-throughput sequencing kinetics for determining enzymatic rate constants of thousands of RNA substrates

Author:
Courtney N. Niland; Eckhard Jankowsky; Michael E. Harris
Source:
Analytical biochemistry 2016 v.510 pp. 1-10
ISSN:
0003-2697
Subject:
RNA-binding proteins; guidelines; high-throughput nucleotide sequencing; ribonucleases; substrate specificity; transfer RNA
Abstract:
... Quantification of the specificity of RNA binding proteins and RNA processing enzymes is essential to understanding their fundamental roles in biological processes. High-throughput sequencing kinetics (HTS-Kin) uses high-throughput sequencing and internal competition kinetics to simultaneously monitor the processing rate constants of thousands of substrates by RNA processing enzymes. This technique ...
DOI:
10.1016/j.ab.2016.06.004
PubMed:
27296633
PubMed Central:
PMC4980219