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You searched for: Journal Analytical biochemistry Remove constraint Journal: Analytical biochemistry Publication Year 2014 Remove constraint Publication Year: 2014 Source 2014 v.449 Remove constraint Source: 2014 v.449 Subject oxidative stress Remove constraint Subject: oxidative stress
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1. Activity assays of mammalian thioredoxin and thioredoxin reductase: Fluorescent disulfide substrates, mechanisms, and use with tissue samples

Author:
Montano, Sergio J.; Lu, Jun; Gustafsson, Tomas N.; Holmgren, Arne
Source:
Analytical biochemistry 2014 v.449 pp. 139-146
ISSN:
0003-2697
Subject:
NADP (coenzyme); active sites; autoregulation; blood serum; cysteine; fluorescein; fluorescence; humans; insulin; oxidation; oxidative stress; substrate specificity; thiols; thioredoxins
Abstract:
... Thioredoxin (Trx) is a protein disulfide reductase that, together with nicotinamide adenine dinucleotide phosphate (NADPH) and thioredoxin reductase (TrxR), controls oxidative stress or redox signaling via thiol redox control. Human cytosolic Trx1 has Cys32 and Cys35 as the active site and three additional cysteine residues (Cys62, Cys69, and Cys73), which by oxidation generates inactive Cys62 to ...
DOI:
10.1016/j.ab.2013.12.025

2. High-performance liquid chromatography determination of glyoxal, methylglyoxal, and diacetyl in urine using 4-methoxy-o-phenylenediamine as derivatizing reagent

Author:
Ojeda, Armando Gómez; Wrobel, Katarzyna; Escobosa, Alma Rosa Corrales; Garay-Sevilla, Ma. Eugenia; Wrobel, Kazimierz
Source:
Analytical biochemistry 2014 v.449 pp. 52-58
ISSN:
0003-2697
Subject:
acetonitrile; acidification; alcoholic beverages; biomarkers; calibration; derivatization; diacetyl; fluorometry; glycation; juveniles; oxidative stress; pH; patients; quinoxalines; reversed-phase high performance liquid chromatography; separation; urine
Abstract:
... Bioanalytical relevance of glyoxal (Go) and methylglyoxal (MGo) arises from their role as biomarkers of glycation processes and oxidative stress. The third compound of interest in this work is diacetyl (DMGo), a component of different food products and alcoholic beverages and one of the small α-ketoaldehydes previously reported in urine. The original idea for the determination of the above compoun ...
DOI:
10.1016/j.ab.2013.12.014