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- Montano, Sergio J.; Lu, Jun; Gustafsson, Tomas N.; Holmgren, Arne
- Analytical biochemistry 2014 v.449 pp. 139-146
- NADP (coenzyme); active sites; autoregulation; blood serum; cysteine; fluorescein; fluorescence; humans; insulin; oxidation; oxidative stress; substrate specificity; thiols; thioredoxins
- ... Thioredoxin (Trx) is a protein disulfide reductase that, together with nicotinamide adenine dinucleotide phosphate (NADPH) and thioredoxin reductase (TrxR), controls oxidative stress or redox signaling via thiol redox control. Human cytosolic Trx1 has Cys32 and Cys35 as the active site and three additional cysteine residues (Cys62, Cys69, and Cys73), which by oxidation generates inactive Cys62 to ...
- Ojeda, Armando Gómez; Wrobel, Katarzyna; Escobosa, Alma Rosa Corrales; Garay-Sevilla, Ma. Eugenia; Wrobel, Kazimierz
- Analytical biochemistry 2014 v.449 pp. 52-58
- acetonitrile; acidification; alcoholic beverages; biomarkers; calibration; derivatization; diacetyl; fluorometry; glycation; juveniles; oxidative stress; pH; patients; quinoxalines; reversed-phase high performance liquid chromatography; separation; urine
- ... Bioanalytical relevance of glyoxal (Go) and methylglyoxal (MGo) arises from their role as biomarkers of glycation processes and oxidative stress. The third compound of interest in this work is diacetyl (DMGo), a component of different food products and alcoholic beverages and one of the small α-ketoaldehydes previously reported in urine. The original idea for the determination of the above compoun ...