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- Vaughan, Robert M., et al. Show all 6 Authors
- Analytical biochemistry 2014 v.463 pp. 70-74
- active sites; acylation; anisotropy; beta-lactamase; fluorescein; hydrolysis; isothiocyanates; meropenem; mutants; penicillins; polyacrylamide gel electrophoresis; proteins
- ... By reacting fluorescein isothiocyanate with meropenem, we have prepared a carbapenem-based fluorescent β-lactam. Fluorescein–meropenem binds both penicillin-binding proteins and β-lactam sensors and undergoes a typical acylation reaction in the active site of these proteins. The probe binds the class D carbapenemase OXA-24/40 with close to the same affinity as meropenem and undergoes a complete ca ...