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- Zhang, Wei-Fen, et al. Show all 5 Authors
- Analytical biochemistry 2016 v.511 pp. 10-12
- Escherichia coli; biocatalysts; biotin; biotinylation; crosslinking; enzyme activity; immobilized enzymes; magnetic separation; magnetism; microparticles; protein-glutamine gamma-glutamyltransferase; recycling
- ... A facile approach for the production of a reusable immobilized recombinant Escherichia coli biotin ligase (BirA) onto amine-modified magnetic microspheres (MMS) via covalent cross-linking catalyzed using microbial transglutaminase (MTG) was proposed in this study. The site-specifically immobilized BirA exhibited approximately 95% of enzymatic activity of the free BirA, and without a significant lo ...