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- Hanff, Erik, et al. Show all 7 Authors
- Analytical biochemistry 2019 v.577 pp. 59-66
- amidinotransferases; analysis of variance; arginase; arginine; catalytic activity; cattle; creatine; enzyme activity; enzyme inhibitors; gas chromatography-mass spectrometry; homeostasis; humans; liver; lysine; metabolism; metabolites; methyltransferases; mortality; nitric oxide synthase; oral administration; stable isotopes
- ... l-Homoarginine (hArg) is biosynthesized from l-arginine (Arg) and l-lysine (Lys) by arginine:glycine amidinotransferase (AGAT). AGAT also catalyzes the formation of guanidinoacetate (GAA) from Arg and glycine (Gly). GAA is converted to creatine (N-methyl guanidinoacetate) by guanidinoacetate N-methyl-transferase (GAMT). Low circulating and excretory concentrations of hArg are associated with worse ...
- Hanff, Erik, et al. Show all 5 Authors
- Analytical biochemistry 2018 v.556 pp. 40-44
- anhydrides; arginine; chemical structure; derivatization; ethyl acetate; gas chromatography-mass spectrometry; humans; ionization; ions; methylation; monitoring; quantitative analysis; risk factors; urine
- ... Circulating and excretory Nᴳ,N´ᴳ-dimethyl-l-arginine (symmetric dimethylarginine, SDMA) and Nᴳ,Nᴳ-dimethyl-l-arginine (asymmetric dimethylarginine, ADMA) are cardiovascular risk factors. Despite close chemical structures, the gas chromatography-mass spectrometry (GC-MS) measurement of SDMA is remarkably more difficult than that of ADMA for as yet unknown reasons. Here, we describe an improved GC-M ...