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- Soheili, Vahid, et al. Show all 6 Authors
- Analytical biochemistry 2018 v.549 pp. 124-129
- antibiotics; binding capacity; bioassays; cost effectiveness; enrofloxacin; fluorescence; graphene oxide; oligonucleotides; raw milk; single-stranded DNA; systematic evolution of ligands by exponential enrichment
- ... Specific ssDNA aptamers for the antibiotic enrofloxacin (ENR) were isolated from an enriched nucleotide library by SELEX (Systematic Evolution of Ligands by EXponential enrichment) method with high binding affinity. After seven rounds, five aptamers were selected and identified. Apt58 with highest affinity and sensitivity (Kd = 14.19 nM) was employed to develop a label-free fluorescent biosensing ...