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- Zhou, Jianzhong, et al. Show all 6 Authors
- Analytical biochemistry 2014 v.445 pp. 14-19
- active sites; fluorescence; fluorescent dyes; gel electrophoresis; high performance liquid chromatography; pH; proteinases; tumor necrosis factor-alpha
- ... A fluorescence-based Adam 17 activity assay that cleaves pro-tumor necrosis factor alpha (pro-TNFα) protein substrate has been developed. The key to the assay was site-specific labeling of a fluorescence dye to the N-terminal end of the substrate protein, which was achieved by the protein ligation method. The protease cleavage reaction was monitored by fluorescence polarization. This homogeneous a ...