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- Hald, Helle; Wu, Boqian; Bouakaz, Lamine; Meldal, Morten
- Analytical biochemistry 2015 v.476 pp. 40-44
- vasoactive intestinal peptide receptors, etc ; cell lines; cyclic AMP; kidneys; plasmids; polypeptides; reporter genes; signal transduction; transfection; yellow fluorescent protein; Show all 10 Subjects
- ... We here present an improved and simplified assay to study signal transduction of the Gs class of G protein-coupled receptors (GPCRs). The assay is based on a single plasmid combining the genes for any Gs protein-coupled GPCR and the cAMP response element-related expression of enhanced yellow fluorescent protein. On transfection, stable human embryonic kidney 293 (HEK293) cell lines presented high ...