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You searched for: Journal name Analytical chemistry Remove constraint Journal name: Analytical chemistry Publication year rev 7978-2022 Remove constraint Publication year rev: 7978-2022 Source 2022 v.94 no.11 Remove constraint Source: 2022 v.94 no.11
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1. Ag–O–Co Interface Modulation-Amplified Luminol Cathodic Electrogenerated Chemiluminescence

Author:
Rui Zou; Ruyu Xie; Yage Peng; Weijiang Guan; Yanjun Lin; Chao Lu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4813-4820
ISSN:
1520-6882
Subject:
adsorption; analytical chemistry; catalysts; coal; electrochemiluminescence; electrochemistry; electrodes; electron transfer; electroplating; indium tin oxide; reactive oxygen species
Abstract:
... It remains a great challenge to develop effective strategies for improving the weak cathodic electrogenerated chemiluminescence (ECL) of the luminol-dissolved O₂ system. Interface modulation between metal and supports is an attractive strategy to improve oxygen reduction reaction (ORR) activity. Therefore, the design of electrocatalysts via interface modulation would provide new opportunities for ...
DOI:
10.1021/acs.analchem.2c00050

2. Biomimic Nanozymes with Tunable Peroxidase-like Activity Based on the Confinement Effect of Metal–Organic Frameworks (MOFs) for Biosensing

Author:
Nuanfei Zhu; Chengbin Liu; Rui Liu; Xiangheng Niu; Dinghui Xiong; Kun Wang; Daqiang Yin; Zhen Zhang
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4821-4830
ISSN:
1520-6882
Subject:
analytical chemistry; bisphenol A; bovine serum albumin; catalytic activity; crystallization; encapsulation; glucose; hydrophobicity; peptides; peroxidase; zeolites
Abstract:
... Biomimic nanozymes coassembled by peptides or proteins and small active molecules provide an effective strategy to design attractive nanozymes. Although some promising nanozymes have been reported, rational regulation for higher catalytic activity of biomimic nanozymes remains challenging. Hence, we proposed a novel biomimic nanozyme by encapsulating the coassembly of hemin/bovine serum albumin (B ...
DOI:
10.1021/acs.analchem.2c00058

3. Biomineralized Zeolitic Imidazolate Framework-8 Nanoparticles Enable Polymerase-Driven DNA Biocomputing for Reliable Cell Identification

Author:
Dan-Dan Wang; Juan Zhang; Qiao-Qin Yu; Ke Zhang; Ting-Ting Chen; Xia Chu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4794-4802
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; cytoplasm; microRNA; therapeutics
Abstract:
... Investigating multiple miRNA expression patterns in living cells by DNA logic biocomputing is a valuable strategy for diagnosis and biomedical studies. The introduction of protein enzymes in DNA logic biocomputing circuits not only expands the toolbox of nucleic acid assembly techniques, but also further improves the specificity of recognizing and processing of DNA input. Herein, a polymerase-driv ...
DOI:
10.1021/acs.analchem.1c05605

4. Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

Author:
Ahmed Mahas; Qiaochu Wang; Tin Marsic; Magdy M. Mahfouz
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4617-4626
ISSN:
1520-6882
Subject:
CRISPR sequences; analytical chemistry; biosensors; diagnostic techniques; dissociation; fluorescence; fluorescent dyes; freeze drying; landscapes; ligands; mobile telephones; single-stranded DNA; tetracycline; transcription factors
Abstract:
... Cell-free biosensors can detect various molecules, thus promising to transform the landscape of diagnostics. Here, we developed a simple, rapid, sensitive, and field-deployable small-molecule detection platform based on allosteric transcription factor (aTF)-regulated expression of a clustered regularly interspaced short palindromic repeats (CRISPR) array coupled to Cas12a activity. To this end, we ...
DOI:
10.1021/acs.analchem.1c04332

5. Fast Discrimination of Sialylated N-Glycan Linkage Isomers with One-Step Derivatization by Microfluidic Capillary Electrophoresis–Mass Spectrometry

Author:
Mengxia Cheng; Hong Shu; Maohua Yang; Guoquan Yan; Lei Zhang; Liang Wang; Wenning Wang; Haojie Lu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4666-4676
ISSN:
1520-6882
Subject:
analytical chemistry; biomarkers; blood serum; capillary electrophoresis; derivatization; humans; mass spectrometry; prediction; quantitative analysis; sialic acid; uterine cervical neoplasms
Abstract:
... Linkage isomers (α-2,3- or α-2,6-linkage) of sialylated N-glycans are involved in the emergence and progression of some diseases, so they are of great significance for diagnosing and monitoring diseases. However, the qualitative and quantitative analysis of sialylated N-glycan linkage isomers remains challenging due to their low abundance and limited isomeric separation techniques. Herein, we deve ...
DOI:
10.1021/acs.analchem.1c04760

6. Gold-Trisoctahedra-Coated Capillary-Based SERS Platform for Microsampling and Sensitive Detection of Trace Fentanyl

Author:
Meiling Zhang; Jialin Pan; Xiaoyu Xu; Gege Fu; Li Zhang; Peng Sun; Xu Yan; Fangmeng Liu; Chenguang Wang; Xiaomin Liu; Geyu Lu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4850-4858
ISSN:
1520-6882
Subject:
analytical chemistry; aqueous solutions; blood serum; cost effectiveness; detection limit; fentanyl; gold; heroin; standard deviation
Abstract:
... A cost-effective and highly reproducible capillary-based surface-enhanced Raman scattering (SERS) platform for sensitive, portable detection and identification of fentanyl is presented. Through encapsulating gold trisoctahedra (Au TOH) in the capillary tube for the first time, the SERS platform was constructed by combining the superior SERS properties of Au TOH and the advantages of capillaries in ...
DOI:
10.1021/acs.analchem.2c00157

7. Isolation Methods Influence the Protein Corona Composition on Gold-Coated Iron Oxide Nanoparticles

Author:
Khoi Nguyen L. Hoang; Korin E. Wheeler; Catherine J. Murphy
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4737-4746
ISSN:
1520-6882
Subject:
Oncorhynchus mykiss; analytical chemistry; centrifugation; iron oxides; isolation techniques; magnetism; mass spectrometry; protein content; protein corona; proteomics; toxicity
Abstract:
... Upon exposure to a biological environment, nanoparticles (NPs) acquire biomolecular coatings, the most studied of which is the protein corona. This protein corona gives NPs a new biological identity that will determine various biological responses including cellular uptake, biodistribution, and toxicity. The standard method to isolate NPs from a biological matrix in order to study their coronas is ...
DOI:
10.1021/acs.analchem.1c05243

8. Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size Exclusion Chromatography for Proteome-wide Cross-Linking Mass Spectrometry

Author:
Ludwig R. Sinn; Sven H. Giese; Marchel Stuiver; Juri Rappsilber
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4627-4634
ISSN:
1520-6882
Subject:
analytical chemistry; crosslinking; gel chromatography; mass spectrometry; peptides
Abstract:
... Ion-mobility spectrometry shows great promise to tackle analytically challenging research questions by adding another separation dimension to liquid chromatography–mass spectrometry. The understanding of how analyte properties influence ion mobility has increased through recent studies, but no clear rationale for the design of customized experimental settings has emerged. Here, we leverage machine ...
DOI:
10.1021/acs.analchem.1c04373

9. Metal-Enhanced Aggregation-Induced Emission Strategy for the HIV-I RNA-Binding Ligand Assay

Author:
Jun Li; Yao-Yao Fan; Jie Wen; Jing Zhang; Zhi-Qi Zhang
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4695-4702
ISSN:
1520-6882
Subject:
RNA; analytical chemistry; fluorescence; genome; humans; immunosuppression; ligands; peptides; therapeutics; transcriptional activation
Abstract:
... The HIV-Ι trans-activation responsive (TAR) RNA–trans-activator of transcription (Tat) protein complex is crucial for the efficient transcription of the integrated human immunodeficiency virus-I genome and is an established therapeutic target for AIDS diagnosis and treatment. Developing a sensitive strategy for the TAR RNA-binding ligand assay could provide antiviral leads with a radically new mec ...
DOI:
10.1021/acs.analchem.1c04889

10. Paper-Based Flow Sensor for the Detection of Hyaluronidase via an Enzyme Hydrolysis-Induced Viscosity Change in a Polymer Solution

Author:
Binglu Zhao; Lubin Qi; Wenjun Tai; Mei Zhao; Xiangfeng Chen; Li Yu; Jianguo Shi; Xiao Wang; Jin-Ming Lin; Qiongzheng Hu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4643-4649
ISSN:
1520-6882
Subject:
analytical chemistry; carcinogenesis; detection limit; humans; hyaluronoglucosaminidase; hypersensitivity; inflammation; medical treatment; pH; paper; polymers; tannins; urine; viscosity; water flow
Abstract:
... Hyaluronidase (HAase) is implicated in inflammation, cancer development, and allergic reaction. The detection of HAase is significantly important in clinical diagnosis and medical treatment. Herein, we propose a new principle for the development of equipment-free and label-free paper-based flow sensors based on the enzymatic hydrolysis-induced viscosity change in a stimuli-responsive polymer solut ...
DOI:
10.1021/acs.analchem.1c04552

11. Precisely Detecting the Telomerase Activities by an AIEgen Probe with Dual Signal Outputs after Cell-Cycle Synchronization

Author:
Rui Liu; Jing-Jing Hu; Xia Wu; Qinyu Hu; Wenlian Jiang; Zujin Zhao; Fan Xia; Xiaoding Lou
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4874-4880
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; apoptosis; electrostatic interactions; fluorescence; hydrogen; interphase; neoplasms; telomerase; telomeres
Abstract:
... By maintaining the telomere lengths, telomerase can make the tumor cells avoid the apoptosis, thus, achieving the cell immortalization. In the past, a series of telomerase detection systems have been developed through utilizing the unique characteristic of telomerase extended primer. However, fluctuation of telomerase activity, along with the cell cycle progression, leads to ambiguous detection re ...
DOI:
10.1021/acs.analchem.2c00583

12. Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker

Author:
Xiao Zhang; Xiaowei Wei; Jijin Qi; Jie Shen; Junwen Xu; Guangyu Gong; Yong Wei; Jian Yang; Qingyi Zhu; Tingting Bai; Zhirui Guo; Xiaojun Qu; Yefei Zhu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4787-4793
ISSN:
1520-6882
Subject:
analytical chemistry; biosensors; blood serum; carbon; detection limit; fluorescence; microRNA; morbidity; mortality; nanogold; urinary bladder neoplasms
Abstract:
... Bladder cancer (BC) is one of the most common cancers in the world, with high morbidity and mortality. It is essential to develop a non-invasive, highly accurate, and simple method for BC diagnosis. This work proposed a fluorescent biosensor based on inorganic nanoflares combined with a DNAzyme walker for the simultaneous detection of BC exosomal microRNAs (miRNAs). This biosensor was constructed ...
DOI:
10.1021/acs.analchem.1c05588

13. Size and Charge Characterization of Lipid Nanoparticles for mRNA Vaccines

Author:
Camille Malburet; Laurent Leclercq; Jean-François Cotte; Jérôme Thiebaud; Emilie Bazin; Marie Garinot; Hervé Cottet
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4677-4685
ISSN:
1520-6882
Subject:
analytical chemistry; capillary electrophoresis; cytoplasm; electrostatic interactions; immune response; lipids; messenger RNA; vaccines
Abstract:
... Messenger RNA vaccines have come into the spotlight as a promising and adaptive alternative to conventional vaccine approaches. The efficacy of mRNA vaccines relies on the ability of mRNA to reach the cytoplasm of cells, where it can be translated into proteins of interest, allowing it to trigger the immune response. However, unprotected mRNA is unstable and susceptible to degradation by exo- and ...
DOI:
10.1021/acs.analchem.1c04778

14. Tandem Probe Analysis Mode for Synchrotron XFM: Doubling Throughput Capacity

Author:
Casey L. Doolette; Daryl L. Howard; Nader Afshar; Cameron M. Kewish; David J. Paterson; Jianyin Huang; Stefan Wagner; Jakob Santner; Walter W. Wenzel; Tom Raimondo; Alexander T. De Vries Van Leeuwen; Lei Hou; Frederik van der Bom; Han Weng; Peter M. Kopittke; Enzo Lombi
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4584-4593
ISSN:
1520-6882
Subject:
analytical chemistry; atomic absorption spectrometry; bioavailability; chemical species; fluorescence; fluorescence microscopy; gels; polyacrylamide; polyurethanes; sediments; soil; transmittance
Abstract:
... Synchrotron-based X-ray fluorescence microscopy (XFM) analysis is a powerful technique that can be used to visualize elemental distributions across a broad range of sample types. Compared to conventional mapping techniques such as laser ablation inductively coupled plasma mass spectrometry or benchtop XFM, synchrotron-based XFM provides faster and more sensitive analyses. However, access to synchr ...
DOI:
10.1021/acs.analchem.1c04255

15. Ultrasensitive Determination of Sugar Phosphates in Trace Samples by Stable Isotope Chemical Labeling Combined with RPLC–MS

Author:
Sha Li; Fei-Long Liu; Zheng Zhang; Xiao-Ming Yin; Tian-Tian Ye; Bi-Feng Yuan; Yu-Qi Feng
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4866-4873
ISSN:
1520-6882
Subject:
Arabidopsis thaliana; analytical chemistry; carbon metabolism; derivatization; detection limit; energy; fructose; glucose; liquid chromatography; mannose; phosphates; protoplasts; stable isotopes
Abstract:
... Sugar phosphates are important metabolic intermediates in organisms and play a vital role in energy and central carbon metabolism. Profiling of sugar phosphates is of great significance but full of challenges due to their high structural similarity and low sensitivities in liquid chromatography (LC)–mass spectrometry (MS). In this study, we developed a novel stable isotope chemical labeling combin ...
DOI:
10.1021/acs.analchem.2c00346

16. Vanadium Species-Assisted Photochemical Vapor Generation for Direct Detection of Trace Tellurium with Inductively Coupled Plasma Mass Spectrometry

Author:
Liang Dong; Hanjiao Chen; Yongyan Ning; Yaowen He; Ying Yu; Ying Gao
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4770-4778
ISSN:
1520-6882
Subject:
acetic acid; analytical chemistry; atomic absorption spectrometry; atomization; detection limit; formic acid; photochemistry; sediments; tellurium; vanadium; vapors
Abstract:
... Photochemical vapor generation (PVG) is emerging as an alternative sample introduction method in the field of atomic spectrometry. The addition of transition metals can largely improve the PVG yields of elements with the enhancement of 1.4 to 30 000-fold, based on previous reports. In this work, the use of vanadium species as novel “sensitizers” in PVG was first reported, tellurium (Te) was select ...
DOI:
10.1021/acs.analchem.1c05525

17. Wash-Free Amperometric Escherichia coli Detection via Rapid and Specific Proteolytic Cleavage by Its Outer Membrane OmpT

Author:
Seonhwa Park; Kiryeon Park; Hyejin Cho; Jungwook Kwon; Kwang-sun Kim; Haesik Yang
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4756-4762
ISSN:
1520-6882
Subject:
Escherichia coli; aminophenols; analytical chemistry; cell membranes; detection limit; electrochemistry; electrodes; leucyl aminopeptidase; peptides; proteolysis; tap water
Abstract:
... Various methods have been developed for the detection of Escherichia coli (E. coli); however, they are complex and time-consuming. OmpT─a cell membrane endopeptidase of E. coli─strongly embedded in the outer membrane of only E. coli, exposed to external solutions, with high proteolytic activity, could be a suitable target molecule for the rapid and straightforward detection of E. coli. Herein, a w ...
DOI:
10.1021/acs.analchem.1c05299

18. A Near-Infrared Probe for Specific Imaging of Lipid Droplets in Living Cells

Author:
Xue Wu; Xiaoxiu Wang; Ying Li; Fanpeng Kong; Kehua Xu; Lu Li; Bo Tang
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4881-4888
ISSN:
1520-6882
Subject:
analytical chemistry; carcinogenesis; ferroptosis; fluorescence; neoplasms; oleic acid; viscosity
Abstract:
... Lipid droplets (LDs) are involved in various physiological processes and associated with cancer development, and are regarded as a potential tumor marker for cancer diagnosis. Monitoring LDs is of prior importance to understand their involvement in biological mechanisms and the early detection of cancers. Highly sensitive and specific noninvasive fluorescent probes are particularly desirable for i ...
DOI:
10.1021/acs.analchem.2c00651

19. A New Ratiometric Design Strategy Based on Modulation of π-Conjugation Unit for Developing Fluorescent Probe and Imaging of Cellular Peroxynitrite

Author:
Lei Wen; Xinyu Ma; Jing Yang; Minmin Jiang; Chao Peng; Zhongyun Ma; Huan Yu; Yinhui Li
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4763-4769
ISSN:
1520-6882
Subject:
analytical chemistry; fluorescence; fluorescent dyes; inflammation; liver; xanthenes
Abstract:
... Ratiometric fluorescent probes could effectively offset the changes of the autofluorescence and compartmental localization. FRET, ICT, etc. are the common strategies to design probes for biosensing, but these strategies have some deficiencies. Here, we proposed a new design strategy based on π-conjugation modulation, giving two different emission bands in the absence and presence of the target. Th ...
DOI:
10.1021/acs.analchem.1c05447

20. A Novel In Situ Method for Simultaneously and Selectively Measuring Asᴵᴵᴵ, Sbᴵᴵᴵ, and Seᴵⱽ in Freshwater and Soils

Author:
Wen Fang; Yi Yang; Paul N. Williams; Haitao Sun; Haiyi Chen; Danxing Yang; Xinyao Shi; Rongbing Fu; Jun Luo
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4576-4583
ISSN:
1520-6882
Subject:
analytical chemistry; antimony; arsenic; freshwater; high performance liquid chromatography; ionic strength; mass spectrometry; pH; porous media; rivers; selenium; silica; soil; solutes; toxicity; watersheds
Abstract:
... Anthropogenic and climatic perturbations redistribute arsenic (As), antimony (Sb), and selenium (Se) within the environment. The speciation characteristics of these elements determine their behavior and biogeochemical cycling, but these redox-sensitive species are challenging to capture, with few methods able to harmonize measurements across the whole plant-soil-ecosystem continuum. In this study, ...
DOI:
10.1021/acs.analchem.1c04243