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1. Analysis of Tertiary Interactions between SART3 and U6 Small Nuclear RNA Using Modified Nanocapillaries

Author:
Youn, Yeoan,   et al.   ; Lee, Choongman; Park, Joon Kyu; Kim, Joo Hyoung; Lee, Kyo-Seok; Kim, Nak-kyoon; Kim, Eunji; Kim, Eunice Eunkyeong; Yoo, Kyung-Hwa;     Show all 9 Authors
Source:
Analytical chemistry 2017 v.89 no.4 pp. 2390-2397
ISSN:
1520-6882
Subject:
DNA; RNA-binding proteins; antigens; binding capacity; calorimetry; dissociation; glass; neoplasm cells; small nuclear RNA; spliceosomes; titration
Abstract:
... We employed modified glass nanocapillaries to investigate interactions between the RNA-binding protein, known as cell carcinoma antigen recognized by T cells-3 (SART3), and the noncoding spliceosome component, U6 small nuclear RNA (snRNA), at the single-molecule level. We functionalized the nanocapillaries with U6 snRNA fragments, which were hybridized to DNA molecules and then covalently attached ...
DOI:
10.1021/acs.analchem.6b04245