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1. Polarizability of Six-Helix Bundle and Triangle DNA Origami and Their Escape Characteristics from a Dielectrophoretic Trap

Author:
Gan, Lin; Camacho-Alanis, Fernanda; Ros, Alexandra
Source:
Analytical chemistry 2015 v.87 no.24 pp. 12059-12064
ISSN:
1520-6882
Subject:
DNA; colloids; dielectrophoresis; drugs; models
Abstract:
... DNA nanoassemblies, such as DNA origamis, hold promise in biosensing, drug delivery, nanoelectronic circuits, and biological computing, which require suitable methods for migration and precision positioning. Insulator-based dielectrophoresis (iDEP) has been demonstrated as a powerful migration and trapping tool for μm- and nm-sized colloids as well as DNA origamis. However, little is known about t ...
DOI:
10.1021/acs.analchem.5b02524

2. One-Step Protein Conjugation to Upconversion Nanoparticles

Author:
Lu, Jie; Chen, Yinghui; Liu, Deming; Ren, Wei; Lu, Yiqing; Shi, Yu; Piper, James; Paulsen, Ian; Jin, Dayong
Source:
Analytical chemistry 2015 v.87 no.20 pp. 10406-10413
ISSN:
1520-6882
Subject:
DNA; biomarkers; biotechnology; chemical bonding; colorimetry; disease diagnosis; drugs; horseradish; hydrophilicity; image analysis; immunoassays; ions; ligands; luminescence; nanoparticles; oleic acid; oxygen; phosphates; phosphoric acid; rare earth elements; solvents; streptavidin
Abstract:
... The emerging upconversion nanoparticles offer a fascinating library of ultrasensitive luminescent probes for a range of biotechnology applications from biomarker discovery to single molecule tracking, early disease diagnosis, deep tissue imaging, and drug delivery and therapies. The effective bioconjugation of inorganic nanoparticles to the molecule-specific proteins, free of agglomeration, nonspe ...
DOI:
10.1021/acs.analchem.5b02523

3. Reusable and Dual-Potential Responses Electrogenerated Chemiluminescence Biosensor for Synchronously Cytosensing and Dynamic Cell Surface N-Glycan Evaluation

Author:
He, Yao; Li, Jinghong; Liu, Yang
Source:
Analytical chemistry 2015 v.87 no.19 pp. 9777-9785
ISSN:
1520-6882
Subject:
DNA; binding proteins; biosensors; blood serum; carbon nitride; concanavalin A; drugs; electrochemiluminescence; electrochemistry; electrodes; humans; nanogold; nanosheets; neoplasm cells; neoplasms; oligonucleotides; screening
Abstract:
... A novel reusable and dual-potential responsive electrogenerated chemiluminescence (ECL) biosensor was fabricated for synchronous detection of cancer cells and their surface N-glycan. In this strategy, a cancer cell recognized aptamer hybridized with a capture DNA was immobilized on electrochemically reduced MoS₂ nanosheets, and Ru(phen)₃²⁺ as ECL probes was intercalated into the grooves of the dou ...
DOI:
10.1021/acs.analchem.5b02048

4. E-DNA Sensor of Mycobacterium tuberculosis Based on Electrochemical Assembly of Nanomaterials (MWCNTs/PPy/PAMAM)

Author:
Miodek, Anna; Mejri, Nawel; Gomgnimbou, Michel; Sola, Christophe; Korri-Youssoufi, Hafsa
Source:
Analytical chemistry 2015 v.87 no.18 pp. 9257-9264
ISSN:
1520-6882
Subject:
DNA; Mycobacterium tuberculosis; biosensors; carbon nanotubes; chemical bonding; detection limit; drugs; electrochemistry; electrodes; electron transfer; genes; gold; nanocomposites; nucleic acid hybridization; pathogens; polymerase chain reaction; polymerization; rifampicin; single nucleotide polymorphism; transducers (equipment)
Abstract:
... Two-step electrochemical patterning methods have been employed to elaborate composite nanomaterials formed with multiwalled carbon nanotubes (MWCNTs) coated with polypyrrole (PPy) and redox PAMAM dendrimers. The nanomaterial has been demonstrated as a molecular transducer for electrochemical DNA detection. The nanocomposite MWCNTs-PPy has been formed by wrapping the PPy film on MWCNTs during elect ...
DOI:
10.1021/acs.analchem.5b01761

5. Time-Resolved Luminescence Biosensor for Continuous Activity Detection of Protein Acetylation-Related Enzymes Based on DNA-Sensitized Terbium(III) Probes

Author:
Han, Yitao; Li, Hao; Hu, Yufang; Li, Pei; Wang, Huixia; Nie, Zhou; Yao, Shouzhuo
Source:
Analytical chemistry 2015 v.87 no.18 pp. 9179-9185
ISSN:
1520-6882
Subject:
DNA; acetylation; acetyltransferases; biosensors; detection limit; drugs; enzyme activity; epigenetics; genes; guanine; histone deacetylase; histones; luminescence; peptides; post-translational modification; terbium
Abstract:
... Protein acetylation of histone is an essential post-translational modification (PTM) mechanism in epigenetic gene regulation, and its status is reversibly controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Herein, we have developed a sensitive and label-free time-resolved luminescence (TRL) biosensor for continuous detection of enzymatic activity of HATs and HDACs, r ...
DOI:
10.1021/acs.analchem.5b01338

6. Reverse Transcriptase in Action: FRET-Based Assay for Monitoring Flipping and Polymerase Activity in Real Time

Author:
Sharma, K. K.; Przybilla, F.; Restle, T.; Boudier, C.; Godet, J.; Mély, Y.
Source:
Analytical chemistry 2015 v.87 no.15 pp. 7690-7697
ISSN:
1520-6882
Subject:
DNA; DNA-directed DNA polymerase; Human immunodeficiency virus 1; RNA; RNA-directed DNA polymerase; antiretroviral agents; drugs; energy transfer; fluorescence; fluorescence emission spectroscopy; genome; hydrolysis; monitoring; nucleosides; nucleotides; radiolabeling; reverse transcription; ribonucleases; screening; therapeutics; working conditions
Abstract:
... Reverse transcriptase (RT) of human immunodeficiency virus-1 (HIV-1) is a multifunctional enzyme that catalyzes the conversion of the single stranded viral RNA genome into double-stranded DNA, competent for host-cell integration. RT is endowed with RNA- and DNA-dependent DNA polymerase activity and DNA-directed RNA hydrolysis (RNase H activity). As a key enzyme of reverse transcription, RT is a ke ...
DOI:
10.1021/acs.analchem.5b01126

7. Chiral Resolution Capabilities of DNA Oligonucleotides

Author:
Tohala, Luma; Oukacine, Farid; Ravelet, Corinne; Peyrin, Eric
Source:
Analytical chemistry 2015 v.87 no.11 pp. 5491-5495
ISSN:
1520-6882
Subject:
DNA; additives; amino acids; aromatic compounds; capillary electrophoresis; drugs; enantiomers; nucleosides; oligonucleotides
Abstract:
... Herein, we studied the chiral resolution properties of a repertoire of arbitrarily chosen DNA oligonucleotides (ON). Ten oligonucleotidic sequences characterized by diverse base compositions, sizes, and structural features, ranging from secondary structure-free homo-oligonucleotides to duplex, hairpin, and three-way junction architectures, were investigated as potential chiral selectors. Their ena ...
DOI:
10.1021/acs.analchem.5b01252

8. Characterization of DNA-Conjugated Compounds Using a Regenerable Chip

Author:
Lin, Weilin; Reddavide, Francesco V.; Uzunova, Veselina; Gür, Fatih Nadi; Zhang, Yixin
Source:
Analytical chemistry 2015 v.87 no.2 pp. 864-868
ISSN:
1520-6882
Subject:
DNA; binding capacity; drugs; high-throughput nucleotide sequencing; organic compounds
Abstract:
... DNA-encoded chemical library (DECL) technology has emerged as a new avenue in the field of drug discovery. Combined with high-throughput sequencing, DECL selection experiments can provide not only many lead compounds but also insights into the structure–affinity relationship. However, the counts of individual DNA codes reflect, but cannot be used to precisely rank, the binding affinities of the co ...
DOI:
10.1021/ac503960z