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1. A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure

Author:
Kobayashi, Norihiro; Banzono, Erika; Shimoda, Yuko; Oyama, Hiroyuki; Kunihiro, Toshiomi; Morita, Izumi; Ohta, Mitsuhiro
Source:
Analytical methods 2011 v.3 no.9 pp. 1995-2002
ISSN:
1759-9679
Subject:
amino acid sequences; analytical methods; biotin; chloroform; cotinine; cross reaction; dose response; enzyme-linked immunosorbent assay; humans; hybridomas; immunoglobulin G; metabolites; mice; monoclonal antibodies; myeloma; nicotine; nitrogen oxides; smoke; splenocytes; streptavidin; tobacco; urine
Abstract:
... Immunoassays for human urinary cotinine [(S)-(−)-cotinine], a major nicotine metabolite, are useful to monitor the degree of tobacco smoke exposure. However, practical monoclonal antibodies for measuring urinary cotinine are not widely available. Here we generated a monoclonal antibody against a newly prepared cotinine–albumin conjugate and developed an enzyme-linked immunosorbent assay (ELISA). S ...
DOI:
10.1039/c1ay05083d

2. A multiclass method for endocrine disrupting chemical residue analysis in human placental tissue samples by UHPLC–MS/MS

Author:
Vela-Soria, F.; Jiménez-Díaz, I.; Rodríguez-Gómez, R.; Zafra-Gómez, A.; Ballesteros, O.; Fernández, M. F.; Olea, N.; Navalón, A.
Source:
Analytical methods 2011 v.3 no.9 pp. 2073-2081
ISSN:
1759-9679
Subject:
adverse effects; animal tissues; atmospheric pressure; benzophenones; bisphenol A; calibration; centrifugation; chemical residues; chemical species; detection limit; endocrine-disrupting chemicals; ethyl acetate; humans; ionization; liquid chromatography; methylparaben; placenta; tandem mass spectrometry; wildlife; women; Spain
Abstract:
... The group of compounds commonly called endocrine-disrupting chemicals covers a wide range of synthetic and natural substances able to alter the normal hormone function of wildlife and humans, consequently causing adverse health effects. Bisphenol A (BPA) and its chlorinated derivatives, benzophenones (BPs) and parabens (PBs), belong to this group of compounds. In this work, we propose a multiclass ...
DOI:
10.1039/c1ay05162h

3. A newly developed salicylanilide functionalized Amberlite XAD-16 chelating resin for use in preconcentration and determination of trace metal ions from environmental and biological samples

Author:
Islam, Aminul; Ahmad, Akil; Laskar, Mohammad Asaduddin
Source:
Analytical methods 2011 v.3 no.9 pp. 2041-2048
ISSN:
1759-9679
Subject:
analytical methods; cadmium; chromium; cobalt; copper; detection limit; fish; green leafy vegetables; hydrogen; lead; mango pulp; metal ions; nickel; pH; sorption; zinc
Abstract:
... Salicylanilide has been incorporated into Amberlite XAD-16 through an azo spacer. Characterization of the synthesized resin was done on the basis of elemental analyses, infra-red spectral and thermal studies. The water regain value and hydrogen ion capacity were found to be 12.90 and 6.08 mmol g⁻¹ respectively. The optimum pH values for the maximum sorption of analyte were 9.0 for Cu(ii), Co(ii), ...
DOI:
10.1039/c1ay05217a

4. An ultra sensitive method for rapid in vitro catalase assay with software based approach using LabVIEW virtual instrumentation

Author:
Kumar JhaPresent address: Department of Nanoscience & Engineering, Myongji University, Yongin, Gyeonggi, South Korea 449-728, Sandeep; D'Souza, Stanislaus F.
Source:
Analytical methods 2011 v.3 no.9 pp. 1981-1987
ISSN:
1759-9679
Subject:
analytical methods; catalase; catalytic activity; cell culture; color; computer software; computers; dissolved oxygen; electrodes; electronic circuits; enzyme activity; foods; hydrogen peroxide; instrumentation; microbial detection; milk; monitoring; oxygen; spectrophotometers; turbidity; viscosity
Abstract:
... A novel approach for estimation of catalase activity in biological samples was developed using real-time signal averaging with LabVIEW based virtual instrumentation. The assay was performed by on-line monitoring of the increase in dissolved oxygen concentration due to the catalytic reaction of the enzyme with its substrate H₂O₂ using a Clark type dissolved oxygen electrode. The electrode was inter ...
DOI:
10.1039/c0ay00760a

5. Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis

Author:
Janssen, Kris P. F.; Knez, Karel; Pollet, Jeroen; Roberts, Scott J.; Schrooten, Jan; Lammertyn, Jeroen
Source:
Analytical methods 2011 v.3 no.9 pp. 2156-2159
ISSN:
1759-9679
Subject:
capillary electrophoresis; gene amplification; oligonucleotides; proteins
Abstract:
... Affinity probe capillary electrophoresis (APCE) holds promise to be a powerful tool in diagnostic applications that rely on the selective and sensitive detection of proteins. Aptamers can serve to unlock the full potential of the technology since their use opens up possibilities to unite the immuno-sensing and separating strength of capillary electrophoresis with the signal enhancement power of nu ...
DOI:
10.1039/c1ay05124e

6. A voltammetric sensor based on electrochemically activated glassy carbon electrode for simultaneous determination of hydroquinone and catechol

Author:
Kong, Yong; Chen, Xiaohui; Yao, Chao; Ma, Mengjie; Chen, Zhidong
Source:
Analytical methods 2011 v.3 no.9 pp. 2121-2126
ISSN:
1759-9679
Subject:
analytical methods; catechol; detection limit; electrochemistry; glassy carbon electrode; hydroquinone; isomers; oxidation; quinones
Abstract:
... A simple and reliable voltammetric sensor for simultaneous determination of hydroquinone (HQ) and catechol (CC) was developed on an electrochemically activated glassy carbon electrode (GCE). The cyclic voltammograms in a mixed solution of HQ and CC have shown that the oxidation peaks become well resolved and were separated by 108 mV, although the bare GCE gave a single broad oxidation peak. Moreov ...
DOI:
10.1039/c1ay05331k

7. Characterisation of plutonium species in alkaline liquors sampled from a UK legacy nuclear fuel storage pond

Author:
Gregson, Colin R.; Hastings, Jeremy J.; Sims, Howard E.; Steele, Helen M.; Taylor, Robin J.
Source:
Analytical methods 2011 v.3 no.9 pp. 1957-1968
ISSN:
1759-9679
Subject:
analytical methods; effluents; encapsulation; isotopes; nuclear fuels; oxidation; plutonium; ponds; radionuclides; silos; sludge; solubility; total suspended solids; United Kingdom
Abstract:
... Within the UK there are a number of nuclear legacy fuel storage ponds and silos that contain substantial volumes of corroding spent Magnox fuel pieces. The fuel and wastes within these ponds, including highly radioactive sludges, must be retrieved and processed during decommissioning. Sludges and other intermediate level wastes will then be encapsulated in a wasteform suitable for storage and disp ...
DOI:
10.1039/c1ay05313b

8. Characterization of Japanese lacquer liquid and films by means of evolved gas analysis-ion attachment mass spectrometry

Author:
Tsukagoshi, Masamichi; Kitahara, Yuki; Takahashi, Seiji; Tsugoshi, Takahisa; Fujii, Toshihiro
Source:
Analytical methods 2011 v.3 no.9 pp. 1943-1947
ISSN:
1759-9679
Subject:
activation energy; liquids; mass spectrometry; temperature; thermal degradation
Abstract:
... We investigated the thermal decomposition of Japanese lacquer liquid and films, which are non-volatile complex natural materials, by means of evolved gas analysis-ion attachment mass spectrometry. The thermal decomposition products are identified by analysis of the mass spectra of degraded samples. The mass chromatogram (pyrogram) of the products generated at various temperatures with kinetic trea ...
DOI:
10.1039/c1ay05215b

9. Determination of some banned aromatic amines in waste water using micellar liquid chromatography

Author:
Mourya, Sandeep-Kumar; Bose, Devasish; Durgbanshi, Abhilasha; Esteve-Romero, Josep; Carda-Broch, Samuel
Source:
Analytical methods 2011 v.3 no.9 pp. 2032-2040
ISSN:
1759-9679
Subject:
chemical species; environmental monitoring; guidelines; health hazards; high performance liquid chromatography; industrial wastes; industry; nitroanilines; pH; pentanols; risk; wastewater
Abstract:
... Aromatic amines are extensively used in industry but they are classified as a potential environment and health hazard. A new high performance liquid chromatography method has been developed and optimized, for the determination of eight banned aromatic amines: benzidine, o-anisidine, o-phenylenediamine, o-nitroaniline, 2-methoxy-5-methylaniline (p-cresidine), o-toluidine, p-toluidine and p-chloroan ...
DOI:
10.1039/c0ay00602e

10. Development and validation of a fast RP-HPLC method to determine the analogue of the thyroid hormone, 3,5,3′-triiodothyroacetic acid (TRIAC), in polymeric nanoparticles

Author:
dos Santos, Karen C.; da Silva, Maria Fátima G. F.; Fernandes, João B.; Vieira, Paulo C.; Polikarpov, Igor; Zucolotto, Valtencir; Forim, Moacir R.
Source:
Analytical methods 2011 v.3 no.9 pp. 1936-1942
ISSN:
1759-9679
Subject:
acetonitrile; lactones; nanoparticles; pH; polyhydroxybutyrate; polymethylmethacrylate; reversed-phase high performance liquid chromatography; temperature; thyroid hormones; triethylamine; wavelengths
Abstract:
... The objective of this work was to develop and validate a rapid Reversed-Phase High-Performance Liquid Chromatography method for the quantification of 3,5,3′-triiodothyroacetic acid (TRIAC) in nanoparticles delivery system prepared in different polymeric matrices. Special attention was given to developing a reliable reproductive technique for the pretreatment of the samples. Chromatographic runs we ...
DOI:
10.1039/c1ay05169e

11. Development of a sequential injection gas diffusion system for the determination of ammonium in transitional and coastal waters

Author:
Segundo, Ricardo Alves; Mesquita, Raquel Beatriz Ribeiro; Ferreira, Maria Teresa Soares Oliveira Barbosa; Teixeira, Catarina Fernanda Carvalho Pinheiro; Bordalo, Adriano Agostinho; Rangel, António Osmaro Santos Silva
Source:
Analytical methods 2011 v.3 no.9 pp. 2049-2055
ISSN:
1759-9679
Subject:
absorbance; ammonia; ammonium; brackish water; coastal water; detection limit; estuaries; green chemistry; salinity; spectroscopy; standard deviation
Abstract:
... This work describes the development of a sequential injection system for the ammonium determination in transitional and coastal waters with a wide salinity range. Estuarine waters are rather complex matrices as their characteristics change considerably along the salinity gradient, as well as the ammonium levels. The developed system effectively solves these issues by converting ammonium into ammon ...
DOI:
10.1039/c1ay05129f

12. Dispersive liquid–liquid micro extraction of uranium(vi) from groundwater and seawater samples and determination by inductively coupled plasma–optical emission spectrometry and flow injection–inductively coupled plasma mass spectrometry

Author:
Chandrasekaran, Krishnan; Karunasagar, Dheram; Arunachalam, Jayaraman
Source:
Analytical methods 2011 v.3 no.9 pp. 2140-2147
ISSN:
1759-9679
Subject:
ammonium; atomic absorption spectrometry; calibration; cetyltrimethylammonium bromide; chelating agents; chloroform; detection limit; fluorometry; graphs; groundwater; hydrophobicity; liquid-phase microextraction; mass spectrometry; nitric acid; pH; seawater; solvents; surfactants; uranium; uranyl ions
Abstract:
... A dispersive liquid–liquid microextraction (DLLME) method was developed for the determination of uranium(vi) in groundwater/seawater by inductively coupled plasma–optical emission spectrometry (ICP–OES) and flow injection–inductively coupled plasma mass spectrometry (FI–ICPMS). This is the first report on the extraction of uranium(vi) by a DLLME method. In this method, uranium(vi) was complexed wi ...
DOI:
10.1039/c1ay05329a

13. 3D ordered gold nanoshell composite array as sensitive SERS nanosensor for detecting l-DOPA and tyrosinase activity

Author:
Rao, Yanying; Chen, Qingfeng; Kong, Fanjuan; Dong, Jian; Qian, Weiping
Source:
Analytical methods 2011 v.3 no.9 pp. 1969-1974
ISSN:
1759-9679
Subject:
4-hydroxybenzoic acid; L-dopa; Raman spectroscopy; cinnamic acid; enzyme activity; enzymes; gold; hydroxylation; nanocomposites; nanogold; nanoparticles; sensors (equipment); silica; tyrosine
Abstract:
... Here we report a novel method for detecting 3,4-dihydroxyphenylalanine (l-DOPA) and tyrosinase (TR) activity based on surface-enhance Raman scattering (SERS) by employing a large-scale versatile three-dimensionally (3D) ordered nanocomposite (SiO₂/GNPs) array as a nanosensor. l-DOPA could reduce AuCl₄⁻ to Au⁰ and enlarge the gold nanoparticles (GNPs) that attach on the surface of the SiO₂/GNPs arr ...
DOI:
10.1039/c1ay05208j

14. Fabrication of adhesive coated swabs for improved swipe-based particle collection efficiency

Author:
Staymates, Jessica L.; Grandner, Jessica; Gillen, Greg
Source:
Analytical methods 2011 v.3 no.9 pp. 2056-2060
ISSN:
1759-9679
Subject:
explosives; fiberglass; narcotics; screening; silicone; spectroscopy
Abstract:
... Improving particle collection efficiency for swipe-based sampling systems can provide a better chance of detection when screening for trace explosives or narcotics particles. A technique was developed to improve the particle collection efficiency of commercially-available collection swabs used for field sampling of trace contraband materials. A silicone adhesive was added to Teflon-coated fibergla ...
DOI:
10.1039/c1ay05299c

15. Gas chromatography-mass spectrometric determination of ivermectin following trimethylsilylation with application to residue analysis in biological meat tissue samples

Author:
Sanbonsuge, Aya; Takase, Tsugiko; Shiho, Den-ichiro; Takagai, Yoshitaka
Source:
Analytical methods 2011 v.3 no.9 pp. 2160-2164
ISSN:
1759-9679
Subject:
ambient temperature; carbon tetrachloride; catalysts; derivatization; detection limit; eprinomectin; gas chromatography-mass spectrometry; horse meat; ionization; ivermectin; liquid chromatography; monitoring; moxidectin; organosilicon compounds; solvents; veterinary drugs
Abstract:
... A gas chromatography-mass spectrometric (GC-MS) method utilizing electron impact ionization has been developed for the quantitation of the antiparasitic agent, ivermectin (IVM). The approach is based upon the pre-column derivatization of IVM by reaction with N,O-bis(trimethylsilyl)tri-fluoroacetamide (BSTFA) in the presence of 1-methylimidazole as catalyst and carbon tetrachloride as solvent to fo ...
DOI:
10.1039/c1ay05236e

16. Graphene-Prussian blue/gold nanoparticles based electrochemical immunoassay of carcinoembryonic antigen

Author:
Wang, Guangfeng; Zhang, Ge; Huang, Hao; Wang, Lun
Source:
Analytical methods 2011 v.3 no.9 pp. 2082-2087
ISSN:
1759-9679
Subject:
analytical methods; antigens; blood serum; detection limit; electrochemistry; graphene; humans; hydrogen peroxide; immunoassays; immunosensors; nanogold; nanoparticles; normal values
Abstract:
... A new electrochemical immunosensor for the determination of carcinoembryonic antigen (CEA) was developed with a Graphene (Gr)-Prussian blue (PB)/gold nanoparticles (GNP) immobilization platform. HRP-anti-CEA was immobilized on the platform and with the immunoreactions, the formed immunocomplex inhibited partly the bioactive center of the immobilized HRP, and decreased the HRP toward the reduction ...
DOI:
10.1039/c1ay05294b

17. Heat-shock transformation of Escherichia coli in nanolitre droplets formed in a capillary-composited microfluidic device

Author:
Sha, Jun; Wang, Yaolei; Wang, Jianchun; Liu, Wenming; Tu, Qin; Liu, Ajing; Wang, Lei; Wang, Jinyi
Source:
Analytical methods 2011 v.3 no.9 pp. 1988-1994
ISSN:
1759-9679
Subject:
Escherichia coli; analytical methods; case studies; droplet size; droplets; genomics; heat stress; microfluidic technology; oils; plasmids; quartz
Abstract:
... This work describes an improved method for monodispersed water-in-oil droplet formation and collection using a composite microfluidic device composed of a poly(dimethylsiloxane) (PDMS) microfluidic device and a commercially available quartz capillary. The application of the method to chemical heat-shock (CaCl₂-dependent) transformation of Escherichia coli (E. coli) is also presented. With this app ...
DOI:
10.1039/c1ay05189j

18. Highly sensitive and selective fluorescent assay for quantitative detection of divalent copper ion in environmental water samples

Author:
Cao, Haiyan; Shi, Wenbing; Xie, Jianxin; Huang, Yuming
Source:
Analytical methods 2011 v.3 no.9 pp. 2102-2107
ISSN:
1759-9679
Subject:
atomic absorption spectrometry; catalytic activity; copper; fluorescence; fluorescence emission spectroscopy; hydrogen peroxide; lakes; oxidation; p-cresol; rivers; standard deviation; swimming pools
Abstract:
... It was found that Cu²⁺ has a great catalytic effect on p-cresol oxidation by hydrogen peroxide under alkaline conditions, leading to an intense fluorescence signal due to the fast formation of 2,2′-dihydroxy-5,5′-dimethylbiphenyl, an oxidation product of p-cresol. Investigation of the fluorescence spectra of the p-cresol–hydrogen peroxide system demonstrated the catalytic behavior of Cu²⁺. On this ...
DOI:
10.1039/c1ay05298e

19. Highly sensitive and selective volatile organic compound gas sensors based on mesoporous nanocomposite monoliths

Author:
Hoa, Nguyen Duc; El-Safty, Sherif A.
Source:
Analytical methods 2011 v.3 no.9 pp. 1948-1956
ISSN:
1759-9679
Subject:
acetone; adsorption; analytical methods; benzene; catalytic activity; cupric oxide; ethanol; ferric oxide; nanocomposites; nanocrystals; nickel oxide; porous media; silica; tin dioxide; transmission electron microscopy; volatile organic compounds; zinc oxide
Abstract:
... We introduce the use of highly ordered mesoporous silica/metal oxide (HOM/MO) nanocomposite monoliths for volatile organic compound (VOC) gas sensor applications. Monoliths with various loadings of semiconducting metal oxides (SnO₂, ZnO, NiO, CuO, and Fe₂O₃) were prepared through instant direct-templating method. The dependence of the doping elements and doping levels on the mesoporous structure o ...
DOI:
10.1039/c1ay05333g

20. Improved sensitive detection of Pb²⁺ and Cd²⁺ in water samples at electrodeposited silver nanonuts on a glassy carbon electrode

Author:
Prakash, S.; Shahi, Vinod K.
Source:
Analytical methods 2011 v.3 no.9 pp. 2134-2139
ISSN:
1759-9679
Subject:
analytical methods; cadmium; detection limit; glassy carbon electrode; lead; metal ions; nanosilver; oxygen; silver; surfactants; wastewater; India
Abstract:
... Silver nanonuts (AgNns) with controlled size were electrodeposited on a glassy carbon electrode (GCE) surface using a double pulse potentiostatic technique for simultaneous detection of Pb²⁺ and Cd²⁺ in water samples without removal of oxygen, surfactant and other metal ions. The AgNns decorated GCE electrode showed improved sensitivity and reproducibility in comparison with previously reported el ...
DOI:
10.1039/c1ay05265a

21. Indirect determination of sodium diclofenac, sodium dipyrone and calcium gluconate in injection drugs using digital image-based (webcam) flame emission spectrometric method

Author:
da Silva Lyra, Wellington; Castriani Sanches, Fátima Aparecida; Antônio da Silva Cunha, Francisco; Gonçalves Dias Diniz, Paulo Henrique; Lemos, Sherlan Guimarães; Cirino da Silva, Edvan; Ugulino de Araujo, Mario Cesar
Source:
Analytical methods 2011 v.3 no.9 pp. 1975-1980
ISSN:
1759-9679
Subject:
analytical methods; calcium gluconate; diclofenac; digital images; drugs; organic matter; sodium; t-test
Abstract:
... This paper proposes a digital image-based flame emission spectrometric (DIB-FES) method for indirect determination of sodium diclofenac, sodium dipyrone and calcium gluconate in injectable forms. The proposed DIB-FES method uses digital images obtained from a webcam, based on the RGB (Red-Green-Blue) system. It offers a simple and inexpensive way to quantify these organic substances using the radi ...
DOI:
10.1039/c1ay05197k

22. Multi-element analysis of bread, cheese, fruit and vegetables by double-focusing sector-field inductively coupled plasma mass spectrometry

Author:
Khouzam, Rola Bou; Lobinski, Ryszard; Pohl, Pawel
Source:
Analytical methods 2011 v.3 no.9 pp. 2115-2120
ISSN:
1759-9679
Subject:
aluminum; antimony; arsenic; atomic absorption spectrometry; breads; cadmium; cheeses; chromium; cobalt; copper; dried milk; fruits; hydrogen peroxide; indium; ions; iron; lead; manganese; mercury; molybdenum; nickel; nitric acid; reference standards; silicon; silver; skim milk; tin; toxic substances; vanadium; vegetables; wheat flour; zinc
Abstract:
... An analytical method using double-focusing sector-field ICP MS was developed for the routine simultaneous multi-element analysis for essential and toxic elements (Ag, Al, As, Cd, Co, Cr, Cu, Fe, Hg, In, Li, Ni, Mn, Mo, Pb, Si, Sb, Se, Sn, V and Zn) in different food samples (bread, cheese, fruit and vegetables). Samples were hot-plate digested with a mixture of concentrated HNO₃ and H₂O₂. Interfer ...
DOI:
10.1039/c1ay05283g

23. Nitrogen factors as a proxy for the quantitative estimation of high value flesh foods in compound products, a review and recommendations for future work

Author:
Thorburn Burns, D.; Walker, Michael; Elahi, S.; Colwell, P.
Source:
Analytical methods 2011 v.3 no.9 pp. 1929-1935
ISSN:
1759-9679
Subject:
analytical methods; cost effectiveness; data collection; fish; ingredients; meat; nitrogen; nitrogen content; poultry; trade
Abstract:
... It is often necessary to verify the quantity of high value flesh ingredients (meat and fish) in compound foods by analysis of the end product. The approach that has stood the test of time in this regard is that originally demonstrated by Stubbs and More who were working in the Laboratory of the Government Chemist (LGC). The method is based on the determination of the nitrogen content of a sample a ...
DOI:
10.1039/c1ay05214d

24. On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Author:
Tsaloglou, Maria-Nefeli; Bahi, Mahadji M.; Waugh, Edward M.; Morgan, Hywel; Mowlem, Matthew
Source:
Analytical methods 2011 v.3 no.9 pp. 2127-2133
ISSN:
1759-9679
Subject:
Karenia brevis; RNA; algal blooms; analytical methods; biotechnology; diagnostic techniques; environmental monitoring; forensic sciences; genes; nucleotide sequences; phytoplankton; point-of-care testing; ribulose-bisphosphate carboxylase
Abstract:
... Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration an ...
DOI:
10.1039/c1ay05164d

25. Poly (Naphthol Green B) film based sensor for resolution of dopamine in the presence of uric acid: A voltammetric study

Author:
Chandra, Umesh; Kumara Swamy, B. E.; Gilbert, Ongera; Sherigara, B. S.
Source:
Analytical methods 2011 v.3 no.9 pp. 2068-2072
ISSN:
1759-9679
Subject:
analytical methods; dopamine; electrodes; films (materials); graphene; pH; phosphates; polymers; uric acid
Abstract:
... A polymer film of Naphthol Green B was prepared on the surface of a graphite pencil electrode using cyclic voltammetry. This poly(Naphthol Green B) coated electrode exhibits an excellent electroanalytical activity towards the determination of dopamine and uric acid. The voltammogram obtained for dopamine at the modified electrode was reversible. The effect of concentration and also pH were investi ...
DOI:
10.1039/c1ay05179b

26. Preparation and evaluation of a novel solid-phase microextraction fiber based on poly(3,4-ethylenedioxythiophene) for the analysis of OCPs in water

Author:
Banitaba, Mohammad Hossein; Mohammadi, Ali Ahmad; Hosseiny Davarani, Saied Saeed; Mehdinia, Ali
Source:
Analytical methods 2011 v.3 no.9 pp. 2061-2067
ISSN:
1759-9679
Subject:
calibration; coatings; desorption; detection limit; gas chromatography-mass spectrometry; gold; headspace analysis; ionic strength; mixing; polymers; porosity; solid phase microextraction; temperature
Abstract:
... A novel polymer coating, based on electropolymerization of poly(3,4-ethylenedioxythiophene) on gold wire is introduced for headspace-solid phase microextraction (HS-SPME) of OCPs and applied for the analysis of these compounds in different water samples by coupling to GC-micro-ECD and GC-MS. The proposed fiber shows good porosity, temperature resistance (up to 300 °C), life time (more than 50 time ...
DOI:
10.1039/c1ay05142c

27. Robust TiO₂/BDD heterojunction photoanodes for determination of chemical oxygen demand in wastewaters

Author:
Han, Yanhe; Qiu, Jingxia; Miao, Yuqing; Han, Jisheng; Zhang, Shanqing; Zhang, Haimin; Zhao, Huijun
Source:
Analytical methods 2011 v.3 no.9 pp. 2003-2009
ISSN:
1759-9679
Subject:
analytical methods; chemical oxygen demand; detection limit; electrochemistry; electrodes; monitoring; nanoparticles; organic compounds; oxidation; photocatalysis; pollutants; titanium dioxide; wastewater
Abstract:
... A TiO₂/BDD heterojunction photoanode, utilizing the inherent properties of nanostructured titanium dioxide (TiO₂) and boron-doped diamond (BDD), was prepared and used to determine chemical oxygen demand (COD) in wastewaters. The TiO₂ nanoparticles were dip-coated on a BDD substrate and subject to calcination processes. A uniform, continuous and robust mixed-phase (anatase and rutile) TiO₂/BDD hete ...
DOI:
10.1039/c1ay05193h

28. Simple method for simultaneous detection of uric acid, xanthine and hypoxanthine in fish samples using a glassy carbon electrode modified with as commercially received multiwalled carbon nanotubes

Author:
Kumar, Annamalai Senthil; Shanmugam, Ranganathan
Source:
Analytical methods 2011 v.3 no.9 pp. 2088-2094
ISSN:
1759-9679
Subject:
analytical methods; calibration; carbon nanotubes; detection limit; electrochemistry; electron transfer; fish; glassy carbon electrode; hypoxanthine; sensors (equipment); uric acid; xanthine
Abstract:
... A simple electrochemical sensor was designed, based on a glassy carbon electrode modified with “as commercially received” multiwalled carbon nanotubes (GCE/MWCNT), for the simultaneous detection of three purine bases: uric acid (UA), xanthine (X) and hypoxanthine (Hx) by differential pulse voltammetry (DPV). Comparison amongst various carbon nanotubes viz.; as commercially received-MWCNT, function ...
DOI:
10.1039/c1ay05065f

29. Solid phase preconcentration and determination of mercury and uranyl ions using an itaconic acid functionalized adsorptive membrane

Author:
Kalyan, Y.; Das, Sadananda; Pandey, A. K.; Naidu, G. R. K.; Sharma, P. K.; Reddy, A. V. R.
Source:
Analytical methods 2011 v.3 no.9 pp. 2017-2024
ISSN:
1759-9679
Subject:
EDTA (chelating agent); Fourier transform infrared spectroscopy; X-ray fluorescence spectroscopy; acetophenones; acrylamides; atomic absorption spectrometry; cadmium; cations; cold; copper; crosslinking; desorption; energy-dispersive X-ray analysis; europium; gravimetry; ion transport; iron; lead; mercury; microgels; mining; pH; photochemical reactions; polymerization; scanning electron microscopy; sodium carbonate; soil sampling; sorbents; uranium; uranyl ions; vapors
Abstract:
... A potential sorbent for preconcentration and quantification of mercury and uranyl ions was developed by grafting cross-linked micro-gel in pores of the host polyethersulfone membrane. The micro-gel was formed by in situ photo-polymerization of itaconic acid (ICA) along with co-monomer acrylamide (AM) and crosslinker pentaerythritoltetraacrylate (PETA) in pores of the host membrane. The photo-polym ...
DOI:
10.1039/c1ay05125c

30. Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy

Author:
Henriques, Cristina; Moreira, Thiago Luiz B.; Maia-Brigagão, Claudia; Henriques-Pons, Andréa; Carvalho, Técia Maria U.; de Souza, Wanderley
Source:
Analytical methods 2011 v.3 no.9 pp. 2148-2155
ISSN:
1759-9679
Subject:
Protozoa; biochemical pathways; color; detection limit; drug therapy; drugs; epimastigotes; flow cytometry; inhibitory concentration 50; metronidazole; mitochondria; oligomycin; screening; tetrazolium; trophozoites
Abstract:
... This study demonstrates the first standardization of the MTS/PMS tetrazolium-based method in T. cruzi and G. duodenalis, and its application for chemotherapy studies and drug screening. We improved the detection limit and compared the MTT, XTT/PMS and MTS/PMS methods, in T. cruzi epimastigotes, demonstrating that MTS/PMS is more reproducible, sensitive, faster and easier to manipulate than XTT/PMS ...
DOI:
10.1039/c1ay05219e

31. The HyperSep SCX micro-cartridge for on-line flow injection inductively coupled plasma atomic emission spectrometric determination of trace elements in biological and environmental samples

Author:
Anthemidis, Aristidis N.; Giakisikli, Georgia; Zachariadis, George A.
Source:
Analytical methods 2011 v.3 no.9 pp. 2108-2114
ISSN:
1759-9679
Subject:
acidity; atomic absorption spectrometry; automation; cadmium; cation exchange resins; chromium; cobalt; copper; detection limit; hydrochloric acid; ions; iron; lead; manganese; nickel; pH; radio waves; reference standards; silver; sorption; zinc
Abstract:
... This work covers an investigation into the potential of developing an automated on-line column preconcentration system using the readily available micro-cartridge HyperSep SCX for fast simultaneous determination of trace Ag(i), Cd(ii), Co(ii), Cr(iii), Cu(ii), Fe(iii), Mn(ii), Ni(ii), Pb(ii) and Zn(ii) ions by inductively coupled plasma atomic emission spectrometry. The proposed method was based o ...
DOI:
10.1039/c1ay05350g

32. Three-phase hollow fiber microextraction based on carrier-mediated transport combined with HPLC-UV for the analysis of dexamethasone sodium phosphate in biological samples

Author:
Ebrahimzadeh, Homeira; Yamini, Yadollah; Ara, Katayoun Mahdavi; Kamarei, Fahimeh
Source:
Analytical methods 2011 v.3 no.9 pp. 2095-2101
ISSN:
1759-9679
Subject:
detection limit; dexamethasone; high performance liquid chromatography; humans; microextraction; milk; pH; sodium phosphate; urine
Abstract:
... A three-phase hollow fiber microextraction technique based on carrier mediated transport combined with high performance liquid chromatography-ultra violet detection (HPLC-UV) was applied in the extraction and determination of dexamethasone sodium phosphate in biological samples. Dexamethasone sodium phosphate (DSP) was extracted from 7.5 mL of acidic solution (source phase) of pH 3 into an organic ...
DOI:
10.1039/c1ay05207a

33. Ultra-performance liquid chromatographic assay coupled with two-dimensional separation for spectrometric determination of urinary S-phenylmercapturic acid

Author:
Lee, Bee Lan; Ong, Her Yam; Chia, Sin Eng; Ong, Choon Nam
Source:
Analytical methods 2011 v.3 no.9 pp. 2025-2031
ISSN:
1759-9679
Subject:
benzene; creatinine; detection limit; diodes; geometry; hydrochloric acid; liquid chromatography; males; metabolites; methylene chloride; monitoring; petroleum; solvents; urine
Abstract:
... A gradient ultra-performance liquid chromatographic (UPLC) assay coupled with the two dimensional separation and diode array detection method was developed to determine S-phenylmercapturic acid (SPMA), a specific urinary metabolite of benzene. Gradient separation was performed on two C₁₈ core–shell columns (2.6 and 1.7 μm, 50 × 2.1 mm i.d.) maintained at 40 and 20 °C. Among various solvents evalua ...
DOI:
10.1039/c1ay05089c

34. Use of online rapid sampling microdialysis electrochemical biosensor for bowel anastomosis monitoring in swine model

Author:
Córcoles, Emma P.; Deeba, Samer; Hanna, George B.; Paraskeva, Paraskevas; Boutelle, Martyn G.; Darzi, Ara
Source:
Analytical methods 2011 v.3 no.9 pp. 2010-2016
ISSN:
1759-9679
Subject:
animal models; batch systems; biosensors; colon; electrodes; flow injection analysis; glucose; ischemia; lactic acid; microdialysis; monitoring; morbidity; mortality; peroxidase; sulfur oxides; surgery
Abstract:
... Bowel anastomosis ischemia carries a significant rise in morbidity and mortality after bowel surgery. Clinical measures of bowel ischemia are often non-specific and only become evident at a late stage. There is currently no method to continuously monitor, in real time, metabolic impairment at the anastomosis site. Our online rapid sampling microdialysis biosensor system has proved its efficacy in ...
DOI:
10.1039/c1ay05306j