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- Helmer, Dorothea; Brahm, Kevin; Helmer, Christian; Wack, Julia Susanne; Brenner-Weiss, Gerald; Schmitz, Katja
- Analytical methods 2016 v.8 no.20 pp. 4142-4152
- N-substituted glycines, etc ; analytical methods; automation; color; exposure duration; fluorescence; fluorescent dyes; humans; image analysis; interleukin-8; ligands; microscopy; photobleaching; proteins; screening; tracer techniques; Show all 16 Subjects
- ... The split-synthesis approach offers a quick and easy method for producing a large diversity of substances for the discovery of novel protein ligands. Screening of the resulting one-bead-one-compound (OBOC) libraries with fluorescently labelled proteins is not trivial, however, since the resin beads can display significant autofluorescence. Here we present a simple two-channel microscopy image-base ...