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1. Droplet-based PCR in a 3D-printed microfluidic chip for miRNA-21 detection

Author:
Jiao, Zhiqiang; Zhao, Lei; Tang, Chu; Shi, Hongyan; Wang, Fu; Hu, Bo
Source:
Analytical methods 2019 v.11 no.26 pp. 3286-3293
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; droplets; organ-on-a-chip; polymerase chain reaction; temperature;     Show all 6 Subjects
Abstract:
... Recently, 3D printing technology has been widely applied in microfluidics due to its capacity of three-dimensional construction, rapid and automatic fabrication, and less time consumption. However, a droplet-based PCR in 3D-printed microfluidic chips is rarely reported. In this study, a 3D-printed microfluidic chip was designed and fabricated for droplet-based PCR detection of miRNA-21. In this ch ...
DOI:
10.1039/c9ay01108k

2. Chemical analysis using 3D printed glass microfluidics

Author:
Gal-Or, Eran; Gershoni, Yaniv; Scotti, Gianmario; Nilsson, Sofia M. E.; Saarinen, Jukka; Jokinen, Ville; Strachan, Clare J.; Boije af Gennäs, Gustav; Yli-Kauhaluoma, Jari; Kotiaho, Tapio
Source:
Analytical methods 2019 v.11 no.13 pp. 1802-1810
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; chemical analysis; drugs; glass; linezolid; manufacturing; mass spectrometry; metals; polymers; production technology; spectral analysis;     Show all 11 Subjects
Abstract:
... Additive manufacturing (3D printing) is a disruptive technology that is changing production systems globally. In addition, microfluidic devices are increasingly being used for chemical analysis and continuous production of chemicals. Printing of materials such as polymers and metals is already a reality, but additive manufacturing of glass for microfluidic systems has received minor attention. We ...
DOI:
10.1039/c8ay01934g

3. A hybrid adhesive bonding of PMMA and PCB with an application on microchip electrophoresis

Author:
Chang, Yongjia; You, Hui
Source:
Analytical methods 2019 v.11 no.9 pp. 1229-1236
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; electronic circuits; electronic equipment; electrophoresis; ions; microchip technology; polymethylmethacrylate; protocols; standard deviation;     Show all 9 Subjects
Abstract:
... In the development of microfluidics, integration of different materials is becoming critical, especially when the integration of fluidic and electronic components is needed. In this work, a hybrid adhesive bonding method combining adhesive and thermal bonding is proposed. It uses a pressure sensitive adhesive (PSA) tape for integration of a polymethyl methacrylate (PMMA) film and printed circuit b ...
DOI:
10.1039/c8ay02642d

4. A simple procedure to produce FDM-based 3D-printed microfluidic devices with an integrated PMMA optical window

Author:
Bressan, Lucas P.; Adamo, Cristina B.; Quero, Reverson F.; de Jesus, Dosil P.; da Silva, José A. F.
Source:
Analytical methods 2019 v.11 no.8 pp. 1014-1020
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; adhesives; analytical methods; chemical structure; glass; models; nitric oxide; nitrites; polylactic acid; polymethylmethacrylate;     Show all 10 Subjects
Abstract:
... Fused deposition modeling (FDM) is the lowest cost 3D-printing technology and has found application in microfluidics in the past few years. However, it lacks resolution and the ability to print transparent windows for optical detection. For this reason, we present a simple protocol to prepare 3D-printed microfluidic channels around 170 μm width and transparent windows using a 0.2 mm nozzle, poly(m ...
DOI:
10.1039/c8ay02092b

5. Compact, inexpensive refractive index detection in femtoliter volumes using commercial optical pickup technology

Author:
Dunn, Robert C.
Source:
Analytical methods 2019 v.11 no.17 pp. 2303-2310
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; electrophoresis; interferometry; ions; lithium; point-of-care systems; proteins; refractive index; silica; sodium; sucrose; wavelengths;     Show all 12 Subjects
Abstract:
... Refractive index (RI) sensing in microfluidics has the advantage of universal detection, capable of sensing all species from simple monoatomic ions to complex proteins without external labels or additional contrast agents. Various forms of interferometry have been developed for RI sensing in microfluidics. In particular, backscatter interferometry (BSI) is easily implemented and well-suited for mi ...
DOI:
10.1039/c9ay00369j

6. Integrating 3D cell culture of PC12 cells with microchip-based electrochemical detection

Author:
Mehl, Benjamin T.; Martin, R. Scott
Source:
Analytical methods 2019 v.11 no.8 pp. 1064-1072
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; dopamine; gold; platinum; polystyrenes; steel; cell culture; chemical species; electrophoresis; models; electrodes; automation; electrochemistry; norepinephrine; pumps; hydrogen; carbon fibers; microchip technology; neurotransmitters;     Show all 19 Subjects
Abstract:
... Developing in vitro cell culture models that accurately mimic in vivo processes in a manner that also enables near real-time analysis of neurotransmitters is an important research area. New technologies being developed such as 3D scaffolds for cell culture and 3D printed microfluidics provide an opportunity for such advancements. In this work, PC12 cells were used as a model system and they were i ...
DOI:
10.1039/c8ay02672f

7. Understanding partial saturation in paper microfluidics enables alternative device architectures

Author:
Buser, Joshua R.; Byrnes, Samantha A.; Anderson, Caitlin E.; Howell, Arielle J.; Kauffman, Peter C.; Bishop, Joshua D.; Wheeler, Maxwell H.; Kumar, Sujatha; Yager, Paul
Source:
Analytical methods 2019 v.11 no.3 pp. 336-345
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; DNA; analytical methods; automation; blood; chemical treatment; equipment; immunoassays; nontarget organisms; nose; paper; point-of-care testing; porous media; urine;     Show all 14 Subjects
Abstract:
... Paper-microfluidic devices are becoming more common due to their ability to automate diagnostic assays at low cost. Increasingly, paper-microfluidic devices automate more complex, multi-step assays, with the aim of bringing these assays out of the laboratory to the point-of-care. However, some common assay procedures have resisted easy automation by paper-microfluidic devices. Sample preparation p ...
DOI:
10.1039/c8ay01977k

8. Microfluidics in coagulation monitoring devices: a mini review

Author:
Harris, Leanne F.; Killard, Anthony J.
Source:
Analytical methods 2018 v.10 no.30 pp. 3714-3719
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; coagulation; decision making; hemorrhage; hemostasis; monitoring; mortality; patient care;     Show all 9 Subjects
Abstract:
... Diagnosis and monitoring of disorders of coagulation and disturbances in haemostasis has been around for many years. Thrombotic and bleeding disorders are amongst the leading causes of mortality in the developed world, thus driving research in this area. The simplicity with which miniaturised devices operate and their application to the study of coagulation and haemostasis offer potential advantag ...
DOI:
10.1039/c8ay01230j

9. A review of centrifugal microfluidics in environmental monitoring

Author:
Maguire, I.; O'Kennedy, R.; Ducrée, J.; Regan, F.
Source:
Analytical methods 2018 v.10 no.13 pp. 1497-1515
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; automation; cost effectiveness; electrochemistry; environmental monitoring; manufacturing; mixing;     Show all 8 Subjects
Abstract:
... There is currently a gap in the use of centrifugal microfluidics in the field of environmental sensing. This review provides theoretical perspectives that have been demonstrated for applications in other domains and recommends adaptations for environmental sensing. Current approaches of centrifugal systems reported are primarily in the area of biosensing and electrochemical detection. In the case ...
DOI:
10.1039/c8ay00361k

10. Microfluidic hydrodynamic trapping for single cell analysis: mechanisms, methods and applications

Author:
Narayanamurthy, Vigneswaran; Nagarajan, Sairam; Firus Khan, Al'aina Yuhainis; Samsuri, Fahmi; Sridhar, T. M.
Source:
Analytical methods 2017 v.9 no.25 pp. 3751-3772
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; acoustics; analytical methods; hydrodynamics; magnetism; markets; trapping;     Show all 7 Subjects
Abstract:
... The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells or particles are trapped in a particular region for single cell analysis (SCA) usually without any application of external force fields such as optical, electrical, magnetic or acoustic. There is a need to explore the insights of SCA in the cell's natural state and developme ...
DOI:
10.1039/c7ay00656j

11. Use of 3D printing and modular microfluidics to integrate cell culture, injections and electrochemical analysis

Author:
Munshi, Akash S.; Chen, Chengpeng; Townsend, Alexandra D.; Martin, R. Scott
Source:
Analytical methods 2018 v.10 no.27 pp. 3364-3374
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; adenosine triphosphate; analytical methods; cell culture; electrochemistry; electrodes; endothelial cells; gold; half life; nitric oxide; polystyrenes; vasodilation;     Show all 12 Subjects
Abstract:
... Fabrication of microchip-based devices using 3D printing technology offers a unique platform to create separate modules that can be put together when desired for analysis. A 3D printed module approach offers various advantages such as file sharing and the ability to easily replace, customize, and modify the individual modules. Here, we describe the use of a modular approach to electrochemically de ...
DOI:
10.1039/c8ay00829a

12. Label-free mesenchymal stem cell enrichment from bone marrow samples by inertial microfluidics

Author:
Lee, Lap Man; Rosano, Jenna M.; Wang, Yi; Klarmann, George J.; Garson, Charles J.; Prabhakarpandian, Balabhaskar; Pant, Kapil; Alvarez, Luis M.; Lai, Eva
Source:
Analytical methods 2018 v.10 no.7 pp. 713-721
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; bone marrow; bone marrow cells; cartilage; mesenchymal stromal cells; mice; orthopedics; stem cells; therapeutics; viability;     Show all 11 Subjects
Abstract:
... Isolation of pure populations of mesenchymal stem cells from bone marrow aspirate is a critical need in regenerative medicine such as orthopedic and cartilage reconstruction with important clinical and therapeutic implications. Currently available stem cell isolation systems mainly rely on intrusive immuno-labeling techniques. Mesenchymal stem cells in bone marrow samples are typically larger than ...
DOI:
10.1039/c7ay02500a

13. 3D-printed microfluidic devices: fabrication, advantages and limitations—a mini review

Author:
Chen, Chengpeng; Mehl, Benjamin T.; Munshi, Akash S.; Townsend, Alexandra D.; Spence, Dana M.; Martin, R. Scott
Source:
Analytical methods 2016 v.8 no.31 pp. 6005-6012
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; equipment design; printers;     Show all 4 Subjects
Abstract:
... A mini-review with 79 references. In this review, the most recent trends in 3D-printed microfluidic devices are discussed. In addition, a focus is given to the fabrication aspects of these devices, with the ESI containing detailed instructions for designing a variety of structures including: a microfluidic channel, threads to accommodate commercial fluidic fittings, a flow splitter; a well plate, ...
DOI:
10.1039/c6ay01671e

14. Droplet microfluidics in thermoplastics: device fabrication, droplet generation, and content manipulation using integrated electric and magnetic fields

Author:
Sahore, Vishal; Doonan, Steven R.; Bailey, Ryan C.
Source:
Analytical methods 2018 v.10 no.35 pp. 4264-4274
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; beta-galactosidase; chemical analysis; droplets; enzyme substrates; geometry; hydrophobicity; magnetic fields; olefin; polymethylmethacrylate; potassium channels; silicon; thermoplastics; washing; wastes;     Show all 15 Subjects
Abstract:
... We have developed droplet microfluidic devices in thermoplastics and demonstrated the integration of key functional components that not only facilitate droplet generation, but also include electric field-assisted reagent injection, droplet splitting, and magnetic field-assisted bead extraction. We manufactured devices in poly(methyl methacrylate) and cyclic olefin polymer using a hot-embossing pro ...
DOI:
10.1039/c8ay01474d

15. Automated 4-sample protein immunoassays using 3D-printed microfluidics

Author:
Kadimisetty, Karteek; Spak, Andrew P.; Bhalerao, Ketki S.; Sharafeldin, Mohamed; Mosa, Islam M.; Lee, Norman H.; Rusling, James F.
Source:
Analytical methods 2018 v.10 no.32 pp. 4000-4006
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; Internet; analytical methods; automation; blood serum; carbon nanotubes; detection limit; diagnostic techniques; enzyme-linked immunosorbent assay; graphene; liquids; nanoparticles; prostate-specific antigen; proteins; rapid methods;     Show all 15 Subjects
Abstract:
... Low cost, miniaturized assay platforms that work with small sample volumes, high sensitivity and rapid detection will have high value in future biomolecular diagnostics. Herein we report an automated, 3D printed electrochemiluminescent (ECL) immunoarray integrated with a nanostructured pyrolytic graphite sheet (PGS) microwell chip configured to detect 2 proteins simultaneously from complex liquid ...
DOI:
10.1039/c8ay01271g

16. Development of immunomagnetic droplet-based digital immuno-PCR for the quantification of prostate specific antigen

Author:
Zhou, Wenhan
Source:
Analytical methods 2018 v.10 no.29 pp. 3690-3695
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; DNA; analytical methods; antigen-antibody reactions; antigens; blood serum; droplets; humans; immunoassays; magnetic fields; models; polymerase chain reaction; prostate-specific antigen; proteins; washing;     Show all 15 Subjects
Abstract:
... Immuno-PCR (IPCR) is an immunoassay that employs DNA as the signal generator and utilizes both the versatility of antigen–antibody interactions and the exponential amplification power of PCR. Droplet-based digital PCR (ddPCR) is the third and latest generation of PCR technology, and the integration of ddPCR into IPCR will hopefully enable the absolute quantification and highly sensitive detection ...
DOI:
10.1039/c8ay00921j

17. Methods to coalesce fluorinated Pickering emulsions

Author:
Pan, Ming; Lyu, Fengjiao; Tang, Sindy K. Y.
Source:
Analytical methods 2017 v.9 no.31 pp. 4622-4629
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; additives; analytical methods; centrifugation; droplets; electric field; emulsions; energy; nanoparticles; polyethylene glycol; surfactants; surveys;     Show all 12 Subjects
Abstract:
... This work surveys methods to coalesce water-in-fluorocarbon emulsions stabilized by amphiphilic nanoparticles (NPs) for applications in droplet microfluidics. Most droplet assays to date have relied on one type of surfactant consisting of perfluorinated polyether and polyethylene glycol (PFPE–PEG) to stabilize the drops against coalescence. Nonetheless, this surfactant is known to have several lim ...
DOI:
10.1039/c7ay01289f

18. Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

Author:
Go, David B.; Atashbar, Massood Z.; Ramshani, Zeinab; Chang, Hsueh-Chia
Source:
Analytical methods 2017 v.9 no.28 pp. 4112-4134
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; atomization; cell adhesion; crystals; liquids; mixing; sounds;     Show all 8 Subjects
Abstract:
... Surface acoustic waves (SAWs) are electro-mechanical waves that form on the surface of piezoelectric crystals. Because they are easy to construct and operate, SAW devices have proven to be versatile and powerful platforms for either direct chemical sensing or for upstream microfluidic processing and sample preparation. This review summarizes recent advances in the development of SAW devices for ch ...
DOI:
10.1039/c7ay00690j

19. Correction: Simplifying microfluidic separation devices towards field-detection of blood parasites

Author:
Holm, S. H.; Beech, J. P.; Barrett, M. P.; Tegenfeldt, J. O.
Source:
Analytical methods 2016 v.8 no.28 pp. 5726
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; analytical methods; hemoparasites; microbial detection; separation;     Show all 5 Subjects
Abstract:
... Correction for ‘Simplifying microfluidic separation devices towards field-detection of blood parasites’ by S. H. Holm et al., Anal. Methods, 2016, 8, 3291–3300. ...
DOI:
10.1039/c6ay90099b

20. Paper-based microfluidics for experimental design: screening masking agents for simultaneous determination of Mn(ii) and Co(ii)

Author:
Meredith, Nathan A.; Volckens, John; Henry, Charles S.
Source:
Analytical methods 2017 v.9 no.3 pp. 534-540
ISSN:
1759-9679
Subject:
microfluidic technology,   etc   ; borates; cobalt; colorimetry; experimental design; iron; ligands; manganese; models; moieties; nickel; pH; paper; phosphates; resorcinol; screening;     Show all 16 Subjects
Abstract:
... This work describes an efficient, generalizable approach towards the identification (and mitigation) of interferences for colorimetric detection using microfluidic paper-based analytical devices. Colorimetric detection is commonly used in conjunction with microfluidic paper-based analytical devices; however, many colorimetric reagents lack selectivity. The selectivity problem is particularly true ...
DOI:
10.1039/c6ay02798a