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- Shipulin, German A., et al. Show all 7 Authors
- Analytical methods 2016 v.8 no.29 pp. 5826-5831
- analytical methods; dyes; fluorescence; hydrophobicity; melting; quantitative polymerase chain reaction
- ... For the first time we used a CuAAC click reaction for the synthesis of cyanine labeled qPCR probes. We performed the comparison of dyes and quenchers (Cy5 vs. disulfo–Cy5 and BHQ2 vs. BHQ3) in several TaqMan probes with different stability of the secondary structure. Based on the studies of thermal quenching of dyes and the increase of fluorescence during probe melting and in the course of qPCR, w ...