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You searched for: Journal Animal biotechnology Remove constraint Journal: Animal biotechnology Publication Year 2015 Remove constraint Publication Year: 2015 Source 2015 v.26 no.2 Remove constraint Source: 2015 v.26 no.2 Subject complementary DNA Remove constraint Subject: complementary DNA Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. MAC-T Cells as a Tool to Evaluate Lentiviral Vector Construction Targeting Recombinant Protein Expression in Milk

Author:
Monzani, Paulo S.; Guemra, Samuel; Adona, Paulo R.; Ohashi, Otavio M.; Meirelles, Flávio V.; Wheeler, Matthew B.
Source:
Animal biotechnology 2015 v.26 no.2 pp. 136-142
ISSN:
1532-2378
Subject:
Western blotting; beta-casein; bovine serum albumin; cattle; complementary DNA; culture media; cultured cells; epithelial cells; gene expression; granulocyte colony-stimulating factor; humans; interleukin-2; mammary glands; milk; polymerase chain reaction; polystyrenes; prolactin; protein synthesis; recombinant proteins; somatotropin; transgenes; transgenic animals
Abstract:
... Prior to generating transgenic animals for bioreactors, it is important to evaluate the vector constructed to avoid poor protein expression. Mammary epithelial cells cultured in vitro have been proposed as a model to reproduce the biology of the mammary gland. In the present work, three lentiviral vectors were constructed for the human growth hormone (GH), interleukin 2 (IL2), and granulocyte colo ...
DOI:
10.1080/10495398.2014.941468

2. Molecular Cloning, Sequence, and Phylogenetic Analysis of T Helper 1 Cytokines of Pashmina Goats

Author:
Shebannavar, Sunil; Rasool, Thaha
Source:
Animal biotechnology 2015 v.26 no.2 pp. 120-129
ISSN:
1532-2378
Subject:
CD4-positive T-lymphocytes; amino acid sequences; amino acids; buffaloes; camels; cats; cattle; complementary DNA; dogs; goats; horses; humans; immune response; interferon-gamma; interleukin-12; interleukin-2; molecular cloning; nucleotide sequences; phylogeny; sequence alignment; sheep; swine
Abstract:
... Cytokines play an important role in regulation of immune responses either in health or disease. In the present study, the cDNAs encoding mature Interleukin (IL)-2, interferon gamma (IFN-γ), and IL-12 p35 and p40 of Pashmina goat were cloned and sequenced. The amino acid sequence was deduced from nucleotide sequence and compared with those available in GeneBank. Mature forms of goat IL-2, IFN-γ, IL ...
DOI:
10.1080/10495398.2013.877022