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You searched for: Journal Animal reproduction science Remove constraint Journal: Animal reproduction science Publication Year 2009 Remove constraint Publication Year: 2009 Source 2009 v.113 no.1-4 Remove constraint Source: 2009 v.113 no.1-4 Subject spermatozoa Remove constraint Subject: spermatozoa
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1. Direct contact between boar spermatozoa and porcine oviductal epithelial cell (OEC) cultures is needed for optimal sperm survival in vitro

Author:
Yeste, M.; Lloyd, R.E.; Badia, E.; Briz, M.; Bonet, S.; Holt, W.V.
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 263-278
ISSN:
0378-4320
Subject:
boars; in vitro fertilization; methodology; spermatozoa; cell culture; culture media; oviducts; cell lines; epithelial cells; optimization; cultured cells; male fertility; viability; sperm motility; acrosome; mitochondria; binding capacity; capacitation; coculture
Abstract:
... Oviductal epithelial cell (OEC) co-culture prolongs sperm viability and motility in vitro in a number of species including humans and horses. This study has sought to determine the effects of homologous OEC co-culture on boar sperm function. To determine whether the effects on spermatozoa were specifically caused by co-culture with or by OEC secretions, or by both factors together, a number of co- ...
DOI:
10.1016/j.anireprosci.2008.08.018
PubMed:
18824315

2. Factors affecting the efficiency of producing porcine embryos expressing enhanced green fluorescence protein by ICSI-mediated gene transfer method

Author:
Wu, Yi; Liu, Cheng-Jun; Wan, Peng-Cheng; Hao, Ze-Dong; Zeng, Shen-Ming
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 156-166
ISSN:
0378-4320
Subject:
swine; transgenic animals; genetic engineering; gene transfer; spermatozoa; injection; oocytes; green fluorescent protein; gene expression; optimization; embryogenesis; blastocyst; binding capacity; culture media; bovine serum albumin; deoxyribonuclease I; temperature; electrical treatment; intracytoplasmic sperm injection
Abstract:
... This study aims to investigate factors that affect the efficiency of blastocyst development and enhanced green fluorescence protein (EGFP) expression in porcine embryos following intracytoplasmic sperm injection (ICSI)-mediated DNA transfer. Frozen-thawed dead spermatozoa were exposed to different concentrations (0.01μg/mL, 0.05μg/mL or 0.1μg/mL) of EGFP DNA solution, and then microinjected into i ...
DOI:
10.1016/j.anireprosci.2008.08.014
PubMed:
18804336

3. Factors influencing the “sortability” of stallion spermatozoa into X- and Y-chromosome bearing populations

Author:
Clulow, J.R.; Evans, G.; Morris, L.H.A.; Maxwell, W.M.C.
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 220-228
ISSN:
0378-4320
Subject:
stallions; assisted reproductive technologies; germplasm conservation; spermatozoa; semen; cryopreservation; sex preselection; X chromosome; Y chromosome; sorting; sperm motility; viability; cell death; freezing quality; freeze-thaw cycles
Abstract:
... Intrinsic differences between stallions exist for semen traits such as motility, morphology fertility and the ability of spermatozoa to survive cryopreservation processes. Ejaculates from 11 stallions were used to test the differences between stallions when selecting X- and Y-chromosome bearing spermatozoa using a modified flow cytometer. Data on orientation and viability of spermatozoa were colle ...
DOI:
10.1016/j.anireprosci.2008.08.019
PubMed:
19022595

4. Identification and sequencing of remnant messenger RNAs found in domestic swine (Sus scrofa) fresh ejaculated spermatozoa

Author:
Yang, C.C.; Lin, Y.S.; Hsu, C.C.; Wu, S.C.; Lin, E.C.; Cheng, W.T.K.
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 143-155
ISSN:
0378-4320
Subject:
spermatozoa; spermiogenesis; animal genetics; molecular genetics; transcription (genetics); transcriptional activation; gene expression; gene expression regulation; swine; complementary DNA; messenger RNA; ejaculation; cDNA libraries; expressed sequence tags; transcriptome; transcriptomics; genome; genomics
Abstract:
... The existence of specific messenger RNA remnants contained within freshly ejaculated spermatozoa was described in several species. Those investigations, using high-throughput techniques to screen the population of transcripts in ejaculated spermatozoa, were limited to the probes which mostly derived from nucleic acids of testicular tissues of either human or mice. The objective of this study was t ...
DOI:
10.1016/j.anireprosci.2008.08.012
PubMed:
18786788

5. Influence of IGF-I on buffalo (Bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro

Author:
Selvaraju, S.; Reddy, I.J.; Nandi, S.; Rao, S.B.N.; Ravindra, J.P.
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 60-70
ISSN:
0378-4320
Subject:
buffaloes; males; assisted reproductive technologies; germplasm conservation; spermatozoa; cryopreservation; culture media; insulin-like growth factor I; male fertility; freeze-thaw cycles; in vitro culture; cell physiology; thawing; plasma membrane; energy metabolism; fructose; nutrient utilization; lipid peroxidation; sperm motility; acrosome; seminal plasma
Abstract:
... The objective of the present experiment was to examine the influence of mean physiological concentration of insulin-like growth factor-I (IGF-I) on frozen-thawed Surti buffalo (Bubalus bubalis) spermatozoa functional parameters, i.e., motility, plasmalemma integrity, acrosomal integrity, functional membrane integrity, lipid peroxidation and fructose uptake in vitro. Frozen-thawed semen samples (n ...
DOI:
10.1016/j.anireprosci.2008.08.011
PubMed:
18804927

6. Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi

Author:
Ding, Shuyan; Ge, Jiachun; Hao, Chen; Zhang, Mingsheng; Yan, Weihui; Xu, Zhiqiang; Pan, Jianlin; Chen, Songlin; Tian, Yongsheng; Huang, Yahong
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 229-235
ISSN:
0378-4320
Subject:
fertilization (reproduction); fish; assisted reproductive technologies; germplasm conservation; spermatozoa; semen; cryopreservation; methodology; semen extenders; cryoprotectants; dimethyl sulfoxide; chemical concentration; temperature; optimization; freezing quality; long term effects; male fertility; freeze-thaw cycles; sperm motility; egg hatchability; China
Abstract:
... In order to develop cryopreservation techniques for long-term preserving the sperm of Mandarin fish Siniperca chuatsi, we examined the effects of various extender and cryopreservation on post-thaw motility. We found the optimal freezing procedures for the Mandarin fish sperm is diluting the semen in D-15 extender, chilling it to 4°C, adding ME₂SO to a final concentration of 10% (v/v), then transfe ...
DOI:
10.1016/j.anireprosci.2008.08.003
PubMed:
18805658

7. Preparation and ultrastructure of spermatozoa from Green Poison Frogs, Dendrobates auratus, following hormonal induced spermiation (Amphibia, Anura, Dendrobatidae)

Author:
Lipke, Christian; Meinecke-Tillmann, Sabine; Meyer, Wilfried; Meinecke, Burkhard
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 177-186
ISSN:
0378-4320
Subject:
frogs; Anura; endangered species; animal reproduction; spermatozoa; acrosome; cell nucleus; flagellum; ultrastructure; in vivo studies; hormonal regulation; spermiation; human chorionic gonadotropin; histology; differential staining; transmission electron microscopy; scanning electron microscopy; phylogeny; Panama
Abstract:
... Few ultrastructural studies have been performed on members of the Dendrobatidae, although such investigations can be useful for the understanding of reproductive patterns, as a diagnostic method for males in breeding programs for endangered amphibians and for phylogenetic analysis. The sperm ultrastructure of the Green Poison Frog, Dendrobates auratus, from Panama is described following induced sp ...
DOI:
10.1016/j.anireprosci.2008.06.005
PubMed:
18657373

8. Seminal traits, suitability for semen preservation and fertility in the native Portuguese horse breeds Puro Sangue Lusitano and Sorraia: Implications for stallion classification and assisted reproduction

Author:
Gamboa, Sandra; Machado-Faria, Manuel; Ramalho-Santos, João
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 102-113
ISSN:
0378-4320
Subject:
stallions; horse breeds; spermatozoa; male fertility; artificial insemination; germplasm conservation; cooling; germplasm evaluation; germplasm screening; sperm motility; pH; breed differences; animal characteristics; Portugal
Abstract:
... The Puro Sangue Lusitano (PSL) is the major national breed of horse in Portugal, but no studies exist on its seminal characteristics, or on the possibility of conserving semen for future use. The aim of this study was to evaluate semen parameters, fertility and the aptness to semen preservation in Lusitano Stallions. In order to compare characteristics defined by a single or by multiple semen coll ...
DOI:
10.1016/j.anireprosci.2008.06.004
PubMed:
18632230

9. Thermoresistance sperm tests are not predictive of potential fertility for cryopreserved bull semen

Author:
Vianna, F.P.; Papa, F.O.; Zahn, F.S.; Melo, C.M.; Dell'Aqua, J.A.
Source:
Animal reproduction science 2009 v.113 no.1-4 pp. 279-282
ISSN:
0378-4320
Subject:
bulls; artificial insemination; germplasm conservation; spermatozoa; semen; cryopreservation; male fertility; prediction; freeze-thaw cycles; thawing; testing; cold tolerance; freezing quality; sperm motility; validity; accuracy; germplasm evaluation; germplasm screening
Abstract:
... Different studies demonstrate positive correlations between seminal variables determined in the laboratory and subsequent fertility after artificial insemination. It is clear, however, that there is still a deficiency in predicting in vivo fertility results of semen samples. The present study intended to verify the efficiency of rapid and slow thermoresistance tests in predicting fertility of froz ...
DOI:
10.1016/j.anireprosci.2008.06.009
PubMed:
18707830