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- Ferreira, R.R.; Meinhardt, L.W.; Azevedo, R.A.
- Annals of applied biology 2006 v.149 no.1 pp. 77
- Sorghum bicolor; grain sorghum; seeds; amino acid metabolism; lysine; threonine; isozymes; molecular weight; aspartate kinase; alcohol oxidoreductases; enzyme activity; free amino acids
- ... Aspartate kinase (AK, EC 220.127.116.11) and homoserine dehydrogenase (HSDH, EC 18.104.22.168) have been partially purified and characterised from immature sorghum seeds. Two peaks of AK activity were eluted by anion-exchange chromatography [diethylaminoethyl (DEAE)-Sephacel] with 183 and 262 mM KCl, and both activities were inhibited by lysine. Similarly, two peaks of HSDH activity were eluted with 145 and 18 ...