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You searched for: Journal name Annals of applied biology Remove constraint Journal name: Annals of applied biology Subject term Western blotting Remove constraint Subject term: Western blotting
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1. Comparison of ELISA and immunoblotting techniques for the detection of cherry mottle leaf virus

Author:
James, D.; Mukerji, S.
Source:
Annals of applied biology 1996 v.129 no.1 pp. 13-23
ISSN:
0003-4746
Subject:
plant viruses; Prunus avium; polyclonal antibodies; Prunus tomentosa; monoclonal antibodies; enzyme-linked immunosorbent assay; Western blotting; detection
DOI:
10.1111/j.1744-7348.1996.tb05727.x

2. Fertile transgenic Lotus corniculatus resistant to the non‐selective herbicide phosphinothricin

Author:
R. Nikolić; S. Zdravković‐Korać; S. Ninković; M. Dragićević; J. Miljuš‐Đukić; B. Banović; B. Bohanec; J. Savić; N. Mitić
Source:
Annals of applied biology 2013 v.163 no.3 pp. 475-493
ISSN:
0003-4746
Subject:
Agrobacterium radiobacter; Lotus corniculatus; Streptomyces hygroscopicus; Western blotting; beta-glucuronidase; cotyledons; explants; flow cytometry; genes; genomics; glufosinate; glutamate dehydrogenase; glutamate-ammonia ligase; greenhouses; herbicide resistance; naphthaleneacetic acid; phosphinothricin acetyltransferase; progeny; seed set; shoots; tillering; weed control; weeds
Abstract:
... Resistance to the non‐selective herbicide dl‐phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co‐cultivation of cotyledons with Agrobacterium tumefaciens AGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding β‐glucur ...
DOI:
10.1111/aab.12071

3. Identification and characterisation of watermelon green mottle mosaic virus as a new cucurbit‐infecting tobamovirus

Author:
Ying‐Huey Cheng; Chih‐Hung Huang; Chung‐Jan Chang; Fuh‐Jyh Jan
Source:
Annals of applied biology 2019 v.174 no.1 pp. 31-39
ISSN:
0003-4746
Subject:
Cucumber green mottle mosaic virus; Western blotting; amino acid sequences; antiserum; coat proteins; cucumbers; electron microscopy; enzyme-linked immunosorbent assay; genes; genomics; leaves; nucleotide sequences; oligodeoxyribonucleotides; rapid methods; reverse transcriptase polymerase chain reaction; seedlings; sequence analysis; virion; viruses; watermelons; Taiwan
Abstract:
... A new virus tentatively named watermelon green mottle mosaic virus (WGMMV) was isolated from watermelon leaves showing mottle, mosaic and leaf crinkling symptoms in Taiwan. Electron microscopy identified tobamovirus‐like, rod‐shaped particles measuring about 18 nm × 300 nm in the crude saps. The enzyme‐linked immunosorbent assay (ELISA) tests of symptomatic watermelon leaves using an antiserum aga ...
DOI:
10.1111/aab.12467

4. Identification of Dictyothrips betae as the vector of Polygonum ring spot virus

Author:
Ciuffo, M.; Mautino, G.C.; Bosco, L.; Turina, M.; Tavella, L.
Source:
Annals of applied biology 2010 v.157 no.2 pp. 299-307
ISSN:
0003-4746
Subject:
Fallopia convolvulus; Frankliniella occidentalis; Polygonum; Thrips tabaci; Western blotting; adults; cytochrome-c oxidase; insects; larvae; leaves; phylogeny; reverse transcriptase polymerase chain reaction; ribosomal DNA; viruses
Abstract:
... Dictyothrips betae (Thysanoptera: Thripidae) is the predominant thrips species on Polygonum convolvulus and Polygonum dumetorum plants infected with a recently described tospovirus species, Polygonum ring spot virus (PolRSV). Laboratory transmission experiments (leaf disk assays) with adults collected directly in the field demonstrated the competence of this thrips to transmit PolRSV, although onl ...
DOI:
10.1111/j.1744-7348.2010.00428.x