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- Author:
- ,; ,; ,; ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- hybrids; homogenization; allozymes; loci; transgenes; transgenic plants; gene flow; genotype; introgression; species diversity; genetic variation; Beta vulgaris subsp. maritima; wild relatives; risk assessment process; beets
- Abstract:
- ... Premise of the study: Agricultural practices routinely create opportunities for crops to hybridize with wild relatives, leading to crop gene introgression into wild genomes. Conservationists typically worry this introgression could lead to genetic homogenization of wild populations, over and above the central concern of transgene escape. Alternatively, viewing introgression as analogous to species ...
- DOI:
- 10.3732/apps.1500114
- PubMed:
- 27011898
- PubMed Central:
- PMC4795919
-
http://dx.doi.org/10.3732%2Fapps.1500114
- Author:
- ,; ,; ,; ,; ,; ,; ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- Baccharis; alleles; conservation areas; essential oils; forests; genetic markers; genetic variation; genomic libraries; heterozygosity; indigenous species; loci; microsatellite repeats; population genetics
- Abstract:
- ... Premise of the study: Baccharis dracunculifolia (Asteraceae) is a native plant of the Atlantic Forest that is used for the production of essential oil. Microsatellite markers were developed for this species to investigate the genetic diversity of three natural populations. Methods and Results: Seventeen out of 27 microsatellite loci identified in a genomic library used for the characterization of ...
- DOI:
- 10.3732/apps.1500093
- PubMed:
- 27011894
- PubMed Central:
- PMC4795915
-
http://dx.doi.org/10.3732%2Fapps.1500093
- Author:
- ,; ,; ,; ,; ,; ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- Odontites; alleles; evolution; gene flow; genetic markers; loci; microsatellite repeats; ploidy
- Abstract:
- ... Premise of the study: Microsatellite primers were developed for the first time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent of gene flow between different ploidy levels.Methods and Results: Fourteen polymorphic and reproducible loci ...
- DOI:
- 10.3732/apps.1500111
- PubMed:
- 27011897
- PubMed Central:
- PMC4795918
-
http://dx.doi.org/10.3732%2Fapps.1500111
- Author:
- ,; ,; ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- Ficus tikoua; alleles; genetic markers; heterozygosity; loci; microsatellite repeats; shrubs
- Abstract:
- ... Premise of the study: Polymorphic microsatellite markers were developed to facilitate studies on the fine-scale population genetic structure of Ficus tikoua (Moraceae), a prostrate shrub known to have highly restricted gene flow.Methods and Results: Microsatellite primers were developed using the biotin-streptavidin capture method and scanned for polymorphism within 76 individuals sampled from thr ...
- DOI:
- 10.3732/apps.1500099
- PubMed:
- 27011895
- PubMed Central:
- PMC4795916
-
http://dx.doi.org/10.3732%2Fapps.1500099
- Author:
- ,; ,; ,; ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- Penstemon; alleles; genetic markers; genetic variation; habitats; heterozygosity; high-throughput nucleotide sequencing; loci; microsatellite repeats
- Abstract:
- ... Premise of the study: Penstemon scariosus var. albifluvis (Plantaginaceae) has been proposed to be federally listed as threatened due to its unique, geologically oil-rich habitat. Developing simple sequence repeat (SSR) markers to study its genetic diversity would be most useful. Methods and Results: Using genomic reduction in combination with next-generation sequencing, we identified SSR motifs w ...
- DOI:
- 10.3732/apps.1500105
- PubMed:
- 27011896
- PubMed Central:
- PMC4795917
-
http://dx.doi.org/10.3732%2Fapps.1500105
- Author:
- ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- Marantaceae; alleles; gene flow; genetic markers; genetic variation; high-throughput nucleotide sequencing; loci; mating systems; microsatellite repeats; screening; Africa
- Abstract:
- ... Premise of the study: Microsatellite markers were developed for the species Haumania danckelmaniana (Marantaceae) from central tropical Africa. Methods and Results: Microsatellite isolation was performed simultaneously on three different species of Marantaceae through a procedure that combines multiplex microsatellite enrichment and next-generation sequencing. From 80 primers selected for initial ...
- DOI:
- 10.3732/apps.1500116
- PubMed:
- 27011899
- PubMed Central:
- PMC4795920
-
http://dx.doi.org/10.3732%2Fapps.1500116
- Author:
- ,; ,; ,
- Source:
- Applications in plant sciences 2016 v.4 no.3
- ISSN:
- 2168-0450
- Subject:
- Clavicipitaceae; Elymus canadensis; alleles; endophytes; fungi; genomics; genotype; single nucleotide polymorphism; translation (genetics); tubulin
- Abstract:
- ... Premise of the study: Primers were designed to produce short amplicons containing single-nucleotide polymorphisms (SNPs) in β-tubulin (tubB) and translation elongation factor 1-α (tefA) in Epichloë canadensis (Clavicipitaceae), an endophytic fungus of Elymus canadensis (Poaceae).Methods and Results: Primers to amplify regions of tubB and tefA containing suspected SNPs were designed and tested on i ...
- DOI:
- 10.3732/apps.1500078
- PubMed:
- 27011893
- PubMed Central:
- PMC4795914
-
http://dx.doi.org/10.3732%2Fapps.1500078